LOCUS       DASOUD010000001        82346 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.001, whole genome shotgun sequence.
ACCESSION   DASOUD010000001 DASOUD010000000
VERSION     DASOUD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.001"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(97..2007)
                     /gene="ltrA"
                     /locus_tag="U4A29_000001"
     CDS             complement(97..2007)
                     /gene="ltrA"
                     /locus_tag="U4A29_000001"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO0679158.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(2791..3996)
                     /locus_tag="U4A29_000002"
     CDS             complement(2791..3996)
                     /locus_tag="U4A29_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO0679159.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(4019..4172)
                     /locus_tag="U4A29_000003"
                     /pseudo
     CDS             complement(4019..4172)
                     /locus_tag="U4A29_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(4175..5560)
                     /locus_tag="U4A29_000004"
     CDS             complement(4175..5560)
                     /locus_tag="U4A29_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO0679160.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(5589..5975)
                     /locus_tag="U4A29_000005"
     CDS             complement(5589..5975)
                     /locus_tag="U4A29_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO0679161.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(5991..6305)
                     /locus_tag="U4A29_000006"
     CDS             complement(5991..6305)
                     /locus_tag="U4A29_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO0679162.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(6658..7137)
                     /gene="rlmH"
                     /locus_tag="U4A29_000007"
     CDS             complement(6658..7137)
                     /gene="rlmH"
                     /locus_tag="U4A29_000007"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO0679163.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            7565..8854
                     /locus_tag="U4A29_000008"
     CDS             7565..8854
                     /locus_tag="U4A29_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO0679164.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(8893..9498)
                     /locus_tag="U4A29_000009"
     CDS             complement(8893..9498)
                     /locus_tag="U4A29_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEO0679165.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(9703..10167)
                     /locus_tag="U4A29_000010"
     CDS             complement(9703..10167)
                     /locus_tag="U4A29_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO0679166.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(10189..10509)
                     /locus_tag="U4A29_000011"
     CDS             complement(10189..10509)
                     /locus_tag="U4A29_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO0679167.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(10653..11732)
                     /gene="pepA"
                     /locus_tag="U4A29_000012"
     CDS             complement(10653..11732)
                     /gene="pepA"
                     /locus_tag="U4A29_000012"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO0679168.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11932..12282
                     /locus_tag="U4A29_000013"
     CDS             11932..12282
                     /locus_tag="U4A29_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO0679169.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            12505..13452
                     /locus_tag="U4A29_000014"
     CDS             12505..13452
                     /locus_tag="U4A29_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO0679170.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(13509..14927)
                     /gene="pepV"
                     /locus_tag="U4A29_000015"
     CDS             complement(13509..14927)
                     /gene="pepV"
                     /locus_tag="U4A29_000015"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO0679171.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            15076..15921
                     /locus_tag="U4A29_000016"
     CDS             15076..15921
                     /locus_tag="U4A29_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO0679172.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15925..16365
                     /locus_tag="U4A29_000017"
     CDS             15925..16365
                     /locus_tag="U4A29_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEO0679173.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            16415..16609
                     /locus_tag="U4A29_000018"
     CDS             16415..16609
                     /locus_tag="U4A29_000018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679174.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            16745..17431
                     /locus_tag="U4A29_000019"
     CDS             16745..17431
                     /locus_tag="U4A29_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO0679175.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(17839..18528)
                     /locus_tag="U4A29_000020"
     CDS             complement(17839..18528)
                     /locus_tag="U4A29_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679176.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(18503..20632)
                     /locus_tag="U4A29_000021"
     CDS             complement(18503..20632)
                     /locus_tag="U4A29_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEO0679177.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(20680..22017)
                     /locus_tag="U4A29_000022"
     CDS             complement(20680..22017)
                     /locus_tag="U4A29_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEO0679178.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(22269..22628)
                     /gene="rplT"
                     /locus_tag="U4A29_000023"
     CDS             complement(22269..22628)
                     /gene="rplT"
                     /locus_tag="U4A29_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO0679179.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(22681..22881)
                     /gene="rpmI"
                     /locus_tag="U4A29_000024"
     CDS             complement(22681..22881)
                     /gene="rpmI"
                     /locus_tag="U4A29_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO0679180.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(22953..23477)
                     /gene="infC"
                     /locus_tag="U4A29_000025"
     CDS             complement(22953..23477)
                     /gene="infC"
                     /locus_tag="U4A29_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO0679181.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(23521..23646)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            23797..24429
                     /locus_tag="U4A29_000026"
     CDS             23797..24429
                     /locus_tag="U4A29_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEO0679182.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(24431..25336)
                     /locus_tag="U4A29_000027"
     CDS             complement(24431..25336)
                     /locus_tag="U4A29_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO0679183.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25421..26080)
                     /locus_tag="U4A29_000028"
     CDS             complement(25421..26080)
                     /locus_tag="U4A29_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO0679184.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            26396..27340
                     /gene="mvk"
                     /locus_tag="U4A29_000029"
     CDS             26396..27340
                     /gene="mvk"
                     /locus_tag="U4A29_000029"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO0679185.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            27333..28310
                     /gene="mvaD"
                     /locus_tag="U4A29_000030"
     CDS             27333..28310
                     /gene="mvaD"
                     /locus_tag="U4A29_000030"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO0679186.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            28323..29408
                     /locus_tag="U4A29_000031"
     CDS             28323..29408
                     /locus_tag="U4A29_000031"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO0679187.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            29405..30469
                     /locus_tag="U4A29_000032"
     CDS             29405..30469
                     /locus_tag="U4A29_000032"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO0679188.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(30718..31521)
                     /locus_tag="U4A29_000033"
     CDS             complement(30718..31521)
                     /locus_tag="U4A29_000033"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEO0679189.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(31541..32383)
                     /gene="kduI"
                     /locus_tag="U4A29_000034"
     CDS             complement(31541..32383)
                     /gene="kduI"
                     /locus_tag="U4A29_000034"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEO0679190.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(32399..33406)
                     /locus_tag="U4A29_000035"
     CDS             complement(32399..33406)
                     /locus_tag="U4A29_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO0679191.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(33413..34057)
                     /locus_tag="U4A29_000036"
     CDS             complement(33413..34057)
                     /locus_tag="U4A29_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO0679192.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(34157..34915)
                     /locus_tag="U4A29_000037"
     CDS             complement(34157..34915)
                     /locus_tag="U4A29_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEO0679193.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(35042..36193)
                     /gene="fucO"
                     /locus_tag="U4A29_000038"
     CDS             complement(35042..36193)
                     /gene="fucO"
                     /locus_tag="U4A29_000038"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEO0679194.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(36275..36586)
                     /gene="rhaM"
                     /locus_tag="U4A29_000039"
     CDS             complement(36275..36586)
                     /gene="rhaM"
                     /locus_tag="U4A29_000039"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEO0679195.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(36588..37433)
                     /gene="rhaD"
                     /locus_tag="U4A29_000040"
     CDS             complement(36588..37433)
                     /gene="rhaD"
                     /locus_tag="U4A29_000040"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEO0679196.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(37447..38703)
                     /gene="rhaA"
                     /locus_tag="U4A29_000041"
     CDS             complement(37447..38703)
                     /gene="rhaA"
                     /locus_tag="U4A29_000041"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEO0679197.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(38700..40160)
                     /gene="rhaB"
                     /locus_tag="U4A29_000042"
     CDS             complement(38700..40160)
                     /gene="rhaB"
                     /locus_tag="U4A29_000042"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEO0679198.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            40313..41182
                     /locus_tag="U4A29_000043"
     CDS             40313..41182
                     /locus_tag="U4A29_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEO0679199.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(41308..42207)
                     /locus_tag="U4A29_000044"
     CDS             complement(41308..42207)
                     /locus_tag="U4A29_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0679200.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(42372..43196)
                     /locus_tag="U4A29_000045"
     CDS             complement(42372..43196)
                     /locus_tag="U4A29_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEO0679201.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(43207..44502)
                     /locus_tag="U4A29_000046"
     CDS             complement(43207..44502)
                     /locus_tag="U4A29_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEO0679202.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(44518..44997)
                     /locus_tag="U4A29_000047"
     CDS             complement(44518..44997)
                     /locus_tag="U4A29_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEO0679203.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(45003..46031)
                     /locus_tag="U4A29_000048"
     CDS             complement(45003..46031)
                     /locus_tag="U4A29_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEO0679204.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(46048..47154)
                     /locus_tag="U4A29_000049"
     CDS             complement(46048..47154)
                     /locus_tag="U4A29_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEO0679205.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(47383..49980)
                     /gene="adhE"
                     /locus_tag="U4A29_000050"
                     /gene_synonym="adhC"
     CDS             complement(47383..49980)
                     /gene="adhE"
                     /locus_tag="U4A29_000050"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO0679206.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(50285..50665)
                     /gene="yajC"
                     /locus_tag="U4A29_000051"
     CDS             complement(50285..50665)
                     /gene="yajC"
                     /locus_tag="U4A29_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO0679207.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(50762..51907)
                     /gene="tgt"
                     /locus_tag="U4A29_000052"
     CDS             complement(50762..51907)
                     /gene="tgt"
                     /locus_tag="U4A29_000052"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO0679208.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            52124..52822
                     /locus_tag="U4A29_000053"
     CDS             52124..52822
                     /locus_tag="U4A29_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO0679209.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(52990..55629)
                     /locus_tag="U4A29_000054"
     CDS             complement(52990..55629)
                     /locus_tag="U4A29_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEO0679210.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(56248..57279)
                     /gene="queA"
                     /locus_tag="U4A29_000055"
     CDS             complement(56248..57279)
                     /gene="queA"
                     /locus_tag="U4A29_000055"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO0679211.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            57472..58647
                     /locus_tag="U4A29_000056"
     CDS             57472..58647
                     /locus_tag="U4A29_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO0679212.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            58651..59292
                     /locus_tag="U4A29_000057"
     CDS             58651..59292
                     /locus_tag="U4A29_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0679213.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            59289..60206
                     /locus_tag="U4A29_000058"
     CDS             59289..60206
                     /locus_tag="U4A29_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO0679214.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            60203..60874
                     /locus_tag="U4A29_000059"
     CDS             60203..60874
                     /locus_tag="U4A29_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0679215.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(61415..61903)
                     /locus_tag="U4A29_000060"
     CDS             complement(61415..61903)
                     /locus_tag="U4A29_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEO0679216.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(62104..62370)
                     /locus_tag="U4A29_000061"
     CDS             complement(62104..62370)
                     /locus_tag="U4A29_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEO0679217.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(62370..62633)
                     /locus_tag="U4A29_000062"
     CDS             complement(62370..62633)
                     /locus_tag="U4A29_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEO0679218.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            63109..63294
                     /locus_tag="U4A29_000063"
     CDS             63109..63294
                     /locus_tag="U4A29_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO0679219.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(63436..65259)
                     /locus_tag="U4A29_000064"
     CDS             complement(63436..65259)
                     /locus_tag="U4A29_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO0679220.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(65857..68562)
                     /locus_tag="U4A29_000065"
     CDS             complement(65857..68562)
                     /locus_tag="U4A29_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO0679221.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(68868..69815)
                     /locus_tag="U4A29_000066"
     CDS             complement(68868..69815)
                     /locus_tag="U4A29_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEO0679222.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            70005..70274
                     /gene="rpsN"
                     /locus_tag="U4A29_000067"
     CDS             70005..70274
                     /gene="rpsN"
                     /locus_tag="U4A29_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO0679223.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(70450..70812)
                     /locus_tag="U4A29_000068"
     CDS             complement(70450..70812)
                     /locus_tag="U4A29_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEO0679224.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(70823..71944)
                     /locus_tag="U4A29_000069"
     CDS             complement(70823..71944)
                     /locus_tag="U4A29_000069"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO0679225.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(71960..72310)
                     /locus_tag="U4A29_000070"
     CDS             complement(71960..72310)
                     /locus_tag="U4A29_000070"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO0679226.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(72440..73951)
                     /locus_tag="U4A29_000071"
     CDS             complement(72440..73951)
                     /locus_tag="U4A29_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO0679227.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(74273..75625)
                     /locus_tag="U4A29_000072"
     CDS             complement(74273..75625)
                     /locus_tag="U4A29_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO0679228.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(75778..76854)
                     /locus_tag="U4A29_000073"
     CDS             complement(75778..76854)
                     /locus_tag="U4A29_000073"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO0679229.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            77050..78795
                     /locus_tag="U4A29_000074"
     CDS             77050..78795
                     /locus_tag="U4A29_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEO0679230.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(79075..79512)
                     /locus_tag="U4A29_000075"
     CDS             complement(79075..79512)
                     /locus_tag="U4A29_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEO0679231.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(79496..80869)
                     /locus_tag="U4A29_000076"
     CDS             complement(79496..80869)
                     /locus_tag="U4A29_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679232.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(81191..81679)
                     /locus_tag="U4A29_000077"
     CDS             complement(81191..81679)
                     /locus_tag="U4A29_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0679233.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(81701..82303)
                     /locus_tag="U4A29_000078"
     CDS             complement(81701..82303)
                     /locus_tag="U4A29_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO0679234.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        23443 a        17598 c        15024 g        26281 t
ORIGIN      
        1 atctcaaact tttcccccca ttcacacgga gcgtgcgact ttcaccgcac tccgcgttcc
       61 atcgtgttta tattgaaaag tatttatgta gtagacctat atcaagttta attcttgatt
      121 gccagctaat actctatatt tatttagtct tatgagctgg actttattta gcttcaatag
      181 agctatatat ttttgtattg tttcttctgt tttagcgtgt attagacggt gtaccgcctc
      241 ttttaccaaa ataagatttt tataatcgtc atttccacca gctccagttg gtacaatatg
      301 atgacaatgt atttcattag cttgttgaaa ttcttccaga gttatagcac attttccatg
      361 ttgagcagaa aatagcgaaa ttcgattgtc tgcgtattct attgtgcgat tttccaaaga
      421 taatttcatt aactggtaca tcagtaattt atcaacactt agattggtgt ggataaattt
      481 tcttccctct agagtgtagc aattcgctaa atacaccttt gccattggtt ttctgtgttg
      541 gatatatcca acagggaaaa taggttcgcc ctcgccagca acataccgaa gcattttaga
      601 tttaccatat cttgttgcct cctttgtcgt aagttgtctg cctgtttttc tcaatctgcc
      661 ccttttgttt gtacctaatc tgttagtgaa gatagccatt acagctctat tcagtttcat
      721 acaatccaga tttacattcg ttgcaatctg ataataattt tgcataccta ataccattga
      781 attataaagt cttgtttctt ctaattccgt tttccctttt gagggagaag caattttctt
      841 tgcctgtgat accagcttgt ctttttggtt tttgaattgt ttatcactta tatgtgattt
      901 aacaactaac ttctttcttc tcggtatcat tttaatcttg aaaccaagaa agtcggagta
      961 tctttttcta acatttacaa ttcttgtttt ttcttgtgat acttccaatt ttaatcgttg
     1021 ttcaatccat tgagtgattg ctattttggc tttttcagca ctttccttgt agcggcagaa
     1081 aactctaaaa tcatctgcat aacgaaccat atacatttcc tttaactttg ttttcttcat
     1141 ttctttgtaa gcgttgcttt tacatgggct accgctttta ttgatatgaa ccacatagtt
     1201 ttttgtaaca ggattttcct gccattggct ttctacccaa tggtctaact cattaagaac
     1261 aatgtttgct agtagtgggg atattattcc cccttgcgga gttcctttct ctggatatgt
     1321 tattgtaccg ttagtcattt tgattggagc ttttaatatc ctttttaaaa tgaatatcaa
     1381 gtgcttgtcc cgtattccca tagcccatat ctgttttatc agcttggaat ggttcacgtt
     1441 gtcaaagaaa cccttaatat caaattcaat cacataatgt agctggctga tttgtaggcg
     1501 gttataggtt gcttttattg cattttcaac tgacctatta ggtctaaaac catagctgtt
     1561 ttcactaaat ttagcttcgc agattggttc taatacttgt tttatacatt gttgtataag
     1621 cctgtcccac atacaaggaa tacctagagg tctggtttta ccatttggtt ttggtatttc
     1681 ttttcgcctc actggcttag gtcgataccc atgtggactg cctgcaacaa tgtatcttac
     1741 ttttttgaca actgtttctg ctggcaattt gccaatatct ttaattattg taccgtctgt
     1801 tcctgctgtt ttactgcctg catttgcttt gatatttcga tatgccagta atatattttc
     1861 tctcgatagg ataatatcca taagatgagt gaaaatatct ccattcttac ttttggcata
     1921 taaatcatca aaggtgtttt gcatttcgta atattcagcg tgtcttaaat cttctacaca
     1981 caatgtttca tttttatttt taggcataag gcttcactcc tttacttgaa attgagtgag
     2041 cttttttatc ttactcgtaa tccttattga aacaacataa tacttttcta atatatacac
     2101 gacttgaggg cattcctcca ttctcattac aagaatttct taggttcgcc ctctgctttt
     2161 cagcattagt tcaactgctt attgttcttc gtcagtttat tctcttgatg agaacccaat
     2221 acctttcctc gttccgataa tcttatctat acatatatat ccttaggtca ctagctataa
     2281 gcctgctggc ttgataccgc ctgtaacgtt atatggcatt tcataaccta tattcttact
     2341 ttgccacacc agctacgatt tctcgtacct ttgcatttct acaaagttca aatttagacc
     2401 cgtacattcg ctgtttcgtc agttccttat agaacattct aaccatagat attttataga
     2461 cccccgacct gttggatacg ctacccacct ctggatagtt ttcgatagaa gatatatgtt
     2521 catcaactat tacggtatct ttcagccgtc tttaccgagc ttcaaacatt ctgagtttcc
     2581 tcatacaatg cttgtcggag gctcagggca ggcgtttcag agcgttactt catcattcca
     2641 cctgtcagat tatcagttct cctaataacc aaactttttc gttcttttat tagtgcctta
     2701 gcttttcact aaggaacgag tcgcacaaag gttttgtcca aaattcataa aaatgttctc
     2761 ctctctatat tcaatgaatt tgtatttgag ttattttttt gttgttatca cgtcctgttc
     2821 ttttactgac tgttgcttca aaatctgctt gtgtcggtct gtcagtttcg catggtcgag
     2881 aatgtctttt acaacctgcg tctggttgat ttcatcaagt ttaatcgcaa cctttaaggt
     2941 cggggcaact tgatgagata gccagttcag cgtcctttgg aaggagtaag gctctggttt
     3001 tgtggttagt tttaatcgtt cacgattgtt cccaataaac caagcccatt cttcattcag
     3061 tttccaatca gaacgaggtt tggaatcgtc tttatctaca aaacggatat accgattgat
     3121 aattttaaag gcggtatgct ctggattgtc atagacgagt aaatcacgga ctgcataata
     3181 ggcacgctca tttttcaatc gaatctcaaa acggtttttt acttctgcgt cttcaatggg
     3241 aatatcattt ttcttgtact gctcgtagtc cttttcatag atacagaaat aaacttcact
     3301 ttgtaatgaa ccgatataga gggtgtttcc catacattcc ttttcctctt tgcgtaccag
     3361 ttcgccactg cgatagcttt taaaactgcg gaagacggag atacattctt cctgttggca
     3421 cttttcagtg agtacaggga tatttaaaat ccctgtctta tcgttaatgg caaggtcaag
     3481 gcgtttcatc acaccgccag ccaccaaaac gtccataaag aactcatacc agcttctttg
     3541 ttgtgccaga agatagcttt caaattgtct gcacccacga cctttcaatt ccaccagaac
     3601 tcctttgtcc agttcatggg agcaaaggac gaatatgtcg cctaaagcat aatgctctga
     3661 ataagaatag aaaccatagt cctcatgaag aaaataggac agtttcagtt gtaagatgtt
     3721 ttcgaccacc tgctgtacgt ctgttgtcgg aaagcgaatt cttacataat caaacagcat
     3781 ttcaagggga gcgtcgggat tgaagcgttc cagagcttcc caaagggact gctgtaaatc
     3841 ctctgatggc ttgacttttc ctgtttcaat atcgcttaga tactgccttg taataccagt
     3901 cgcaacagct aaacggtttt gagatagtcc ataagccaag cgtttttctt ttaaatgctg
     3961 taaccaagtt tgttcattca gtaaaaatcc ctccaatcaa aaaggcgtat gtcaactttt
     4021 aaagcccatt tgacatacgc tgaaattttg taaatccctt gtaaccaaag gattttctaa
     4081 tgtttttttg actgtttcct gtcgatttgt acccccctgt tagataccga ggggttaagt
     4141 gctggcgtgg ctattgccac accagccagc aagatcagtc cacacctgcg acttccgctt
     4201 cgcacgtcgc ctgcgtggac tgtctgctgt tggataactt tttaatttcc tccaagaaat
     4261 catatccttt tggtacaagg ggagtataaa actctgatat gacacttgtt cctacatcaa
     4321 catagccacg acctttgatt cgctttaaga agaaatcctt ttgtacgtca ctgccaaaca
     4381 tcatgccata gcccatttca gacatacgac ctaaagccac tctgaaatta aactgatcac
     4441 ggattccgtc gcctaaatat tttgcgtctg gacgttgaca agccagtatt agaaagaagc
     4501 cagcttgacg acctaacatg acaatctgtt tcagcttatt cataactgcg gtgttttctt
     4561 ttgttcccag catttccatg aaagcgacgt attcatcaaa gattaagaag tgtgccggga
     4621 gacctaagta agcataattt ttgccagtct tatagttctt catctgcttc atttcctcac
     4681 tacgtttcat catttcttca tagaatgttt caatgcaaga aagcaagtct tcttttctat
     4741 agtagacatt tgccatcaca gaacctaagt ccgcaagatc agcatttttc gggtcaagaa
     4801 tatacagttt tgaatctgta tgaagcaagg cttcaatcag tgtcagtata aagtaagttt
     4861 taccgccacc tgtaccacca gcaatcaaca tatgagggag cttatcatat tcccaccata
     4921 cgtttttcat taagcgaagt ttaccatctt tagcttctac ttcatcaata gaaatacgac
     4981 tggctatggt gtcatagagc aaagtatatt ccacatagga atcctttaac tctttatccg
     5041 tcagctcaca gtacaagcca ctctctaatt tcttttccaa gtgtaagagt tggtcttgat
     5101 attttcccag cgtgatttcc acccgtatct gtatcaagcc atttttaagt cgataataca
     5161 ttttagggaa gtaggttatc ttttcctttg tacgaccagc actatcttta aagaaaccct
     5221 ctgttttgac ctgttcagat tcataccact tgttttcaag tatcatcttt gccagttttt
     5281 gacggtggta aagttgttta accgtatcat agcgaacccg tttgaataca aacgctacca
     5341 gcaagcagat aagaattgcg acactgaaac tgataattaa atagggaatg tcaatcttat
     5401 ctgcttgtga taggttaaaa tcctgccagt tgatctgctg gattgtcttc acatgaaaca
     5461 gtccgacaac cagcaggaaa acaggcagga gtgacgctat cgtaaaatga aagactaaat
     5521 ctttaccaga tgggcgaatc cttttaccac gctgtttcat gcgaaaaagt ctcctttcta
     5581 cctagcgact atttgtcttg tgtcggttct ttctttgctt gtggttgagc tttgaatgaa
     5641 ctagaatcct ttgtcagcac aatatcgtct gccttgatat accagtcaac atctgctcct
     5701 tgataggtgg cagtagcaac ggtgtccgca atgggattga taagttccac ccgtgcgtta
     5761 taatcaaact ctttcaaagg cacgctggca ggaatactta cttgaatcat gcgtccttgt
     5821 cctttggatt ttaagtcata ggtacgttcc ttgatttcat ctgaaaccga cccgtcttca
     5881 ttttggattc tcacttcacg acgtagagca gagaatttca attctccaaa agtcgtgtct
     5941 ttatctaata caatgccatt tgctaatctc atcatttttc ctctctttct ttattctttt
     6001 atcatgtcgt cagcatgtaa aaggtaattt gtaaaaccac gagtgccgat tttgtagccc
     6061 tctgcggtaa tacgtggatt gactaacttc acacgttcct caaagccgaa atgtttttcg
     6121 ccagcttcag caggaagcac caccacaata tcatctgctc tttgaacatc agaatagaga
     6181 ttatagcttc ttgataagac agttagccgt ccgttgattc ttcgctgaac gactttatcc
     6241 tcgccagcaa attctaaatt gccgaatgtt ttttccatgt tgggaatcac aaatttaagt
     6301 tccatatttt tacctatcct ttctttttta ttggctgaat gaatgtttga tggtcttaaa
     6361 gagtggggaa cgaccttttg attcttgatt ttttgttttc ataagttcac ttcctttcaa
     6421 aatcgggtaa aaaaatagac acctcatttt ttgaagtgtc tacctattaa atattcaaat
     6481 tttattggaa gtatctttat atcttcactt ttcaaggata aatcgtcgta tcaaagctca
     6541 ttcataagta gtaaattagt agtaaattga gtggttttga ccttgataaa gtgtgataag
     6601 tccagttttt atgcggataa ctagattttt atgctatttt tctcttaaaa aatatcatta
     6661 tttgtggtat ggagcccccg cgttgatccg gaaggcacgg tagatttgct cgacgaggat
     6721 caggcgcatc aattgatgtg gatacgtcat tttgccaaaa gagatctgag cattgcttct
     6781 tttcatgacg gcttcgctga gtcctaaaga accgccgata acaaaaacaa ggtggctttt
     6841 tccttggata cctaattggt cgatttgttt agcaaaagat tcgcttgtgg gatttttccc
     6901 ttcaattgct aagccataga cgtattcttg ttctttgatt ttcgctaaga tccgttctcc
     6961 ttctttttct tttacttgtc tcatttgcgc ttcgcttaaa ttctccgggg ctttttcgtc
     7021 aggaacttcg atcaattcga ttttcgcata ggcattcagc cgtttgacat actcattgat
     7081 accttggatc aagtattttt cttttaattt tcctactgaa ataattttga tattcatgtt
     7141 ttctctcctt tgatcttcac gctcactttg tttattataa tcaatttttt cgtctatatg
     7201 tttattttga ctatttttgt taaaaagatt tccccaacag ttatgcacaa catccacagg
     7261 tttatccaca gaaaacagcg ttgttttacc ttatttcatc acattcatta ggcaaacata
     7321 cgttttataa gaatttatcc actttataca caggctgtgg atatgtttta tcccttattt
     7381 ttccaatcct atccgcgtag ttttacacaa tatctgtgga aaaagaaaat caaatatttt
     7441 catctgaaaa caatgatttt tcctaaagtt tttcataaaa agttcaaagt ctcaaagtag
     7501 actaaagtca acaaataaga ctatactaac agtagataaa tgaatgtaat tggaggagga
     7561 acacatggat agaaaaaatg tgacaccaaa gatgaaaaaa aataaaaata atagcctctg
     7621 gcgtaagttg ggtctggggc ttgttggagg gatcgttgga ggactactga cagctggcat
     7681 tttttatgca gcgatgggca ctggcaacaa tagttctact gctacaagcg gcaatcagaa
     7741 ttctgccggt gaaacagttg tggaaaatgt caaagtcaat gtagattctg atatcaccaa
     7801 agcggtagat aaagttcaag acgctgtcgt ttctgttatc aacttacaaa gtcaaaacca
     7861 atcaagcggt tttggcggac tattcgggca gcaagaagaa agtagcagct cagatgatag
     7921 taatttggaa gcttatagtg aaggaagcgg cgtgatctac aaaaaagacg ggaatactgc
     7981 ttatgtagtc acgaataatc acgtagtaga tggccagcaa ggtcttgaag tcttgatgaa
     8041 agacggtacg aaggtaaaag ctgaattagt tggaacagat gcttattcag atttagcggt
     8101 gttgaagatc aattcagaca aagtcgaaac agttgcctct tttggtgact caagtgctct
     8161 gaaggttggt gagcctgcga ttgcgattgg ttccccacta ggatctgaat atgcaaattc
     8221 tgtcacttca ggaatcattt cttccttgaa ccgacaagtg acaagtacga atgaatcgaa
     8281 cgaaacagtc aatatcaatg cgatccaaac agatgcagca atcaaccctg gaaattctgg
     8341 tggtccttta gttaatatcg aaggacaagt tatcggtatc aactcaagta agatcgcaag
     8401 tacttctgaa tctacttcta atgtaagcgt agaaggaatg ggatttgcta ttccaagtaa
     8461 cgatgtagtg aatatcatca atcaactgga aaaagacgga aaagtgacac gtccagctct
     8521 aggtatcaca atggtcgact tatctgccgt ttctactcag caacaagaac aaatcttgaa
     8581 gattccttca tctgtaacta acggtgtcat cgtgacaaat gtccaaacag ctacaccggc
     8641 tgaaaaagct ggattgaaac aatacgacgt cattactaag atcgatgaca aagaagtttc
     8701 ttctggtgta gaacttcaat ctgctttgta taagaaaaaa gtgggagatt ctgtcaaaat
     8761 cacttactat gaaggctcga aagaaaaaac aactactgtt gaattgacga tcgatcaatc
     8821 ggcattaaaa caaaatcaaa atagtgaaaa ttaataaaaa agaggttgtg acaaagactt
     8881 gtcacaacct ctttattttg gaaatgcttc tccgactacg gcatcctcag gcaatactaa
     8941 gattcctttt tttgctggag catttggcaa atgcaattca cgagcggaac agatcattcc
     9001 atagctatct acccctcgca aagttcctgg ccagatcatc attccatctg gcatcatagc
     9061 tcctggtttt gcaacgacaa ctttttgtcc agcttcgata ttgggtgcac cgcaaacgat
     9121 ttgcaatact gtaccattgt ctacttctgt ctcagtaatc gataaatggt cagaatcagg
     9181 atgtgggacg catgttttta cgtacccgac aacgatcttc ggttcatgat cagcttcaag
     9241 ctgttcttcg aatcctgctt ctttgatcaa tcgattcaat ttcgctaatt cctcatctgt
     9301 taagcttact tggccgttcc cttcgattgt tagatgatcg gagatatgga aaaagttcca
     9361 agcaacaact tgaccttgtt catctttgat ccgagcaaca ttccatttgc gttcaacagt
     9421 gttttctgtt cctttatcgt cggctataat aaccatcaag gtgtctccga cgtgtgcttt
     9481 gttataagca aaaatcatgt tctttcctcc attatccttt gctgagattt atcttaacaa
     9541 aaaattgcat tcactgcatg aaattttttt catgctttat ttccgaagaa gcctcttctg
     9601 gaacaccatt tattcgtaac aagggactgt gacaagactt ttgtcacagt ccctttactg
     9661 ttttagttaa ttgattaaaa aggtcgtaca agttccactt gcttaattac ttggctgttc
     9721 ttcttcgtca gaaacgatca agacatcaca aggtgaatga cgtatcacgt agctggctac
     9781 acttcctgtc ataaagcgtt cgactgcatt tagacctgac tgcccaacca tgattagatc
     9841 gatctcatat ttttttggca atttgactgc catggattca cgaacagaac catatgcaat
     9901 aattccttct atatttgtgt aatcaacaga tttcccatat tctttacatt cttcgatcat
     9961 ttccttggct gtttcagttt gctgatcgac tagattttga tcaataggtg catagcccat
    10021 aaagttataa aattgctgat cgattacgtt tgctacatat actttcccat tattacggcg
    10081 agccacttct accgcttttt tgaaagcagc aagtgcttgt tcactaccgt cgatacctac
    10141 taaaatattc ttatattctt gattcatctt gacccctcct aacagatgtc agccttttgt
    10201 tacactttct aggaacgatt cgatttcctg ttttgtttta cgatctttat tcactagacg
    10261 acctaattct tttccgtctt ggatcacgac aaaacttggg ataccaaaaa tgttccattc
    10321 agcggctaca tctatgtact tgtcacggtc tacttcaata aattgccaat cagaaaagtc
    10381 attttcaatt tcaggcattt gcggtttgat aaaacggcag tctccgcacc agccggcagt
    10441 aaagaaaaat acactctttc cagtttctac gtagcctgct aattcttcta aactatttgg
    10501 gataatcatt ctttgtcctc tactttctgt ttatgtattt ttcaattaaa ttatacacct
    10561 ttctttcgaa ctaacaaaca ttgatcctct ttttataaaa aaaatctggc aaaaagcaat
    10621 cttttgccag attttatagt ttcactatcg cattattttc cgtatacgat cgtatttact
    10681 gttgatttat ccaaaccttt cagtgtttga atcaacattt ctcgtgcagc ttcaaaatcg
    10741 cgaatactga acatcgtttg atgcgtatgg atataacgtc ccactacgcc aatcactgta
    10801 cttgggattc cttcgttttg tgtatgagca gcacctgcat ctgtaccgcc ttttgaaaca
    10861 aagtattgat aagggatatt gtttgtttca gcgatatcca gcaagtactc gcgcaaacga
    10921 ggaagcatga tcaaacctgg atcttggata cgcatcaatg taccttctcc taaatgtccg
    10981 aatgtgccat ttttcgtcac tgtatcatct gctgcagagc agtctactgc aaagaacaga
    11041 tctggcttga atttattgac agaagcttta gaaccacgca agcctacttc ttcctggaca
    11101 ttcgcgcctg caatcaatgt atgacctaat gtttcatttt gcaacgcttc taaagcttct
    11161 aataccattg tacagccata gcggttgtcc catgatttac tgataatatt ctttccgttg
    11221 gctgttttga tcgtttcagc gtaaggaacg attgtgtctc ctggaaggac accgtaactc
    11281 attgcctctt ctcttgattc aaacccagca tcgaacaaaa tatcggttac ttctactgat
    11341 ttttgtccgc ttgttcctcg caataaatgt gggggaacgg aagaagaaat acatggataa
    11401 ttcttaccag tagaggtttt aagtgtaaaa cgctgagcag aaacaacgta aggattccag
    11461 ccgcctagag gtacgacttt aaaaagcccg ttttcttgga tttgtgtcag catgaatccg
    11521 acttcatcca tatgtgctgc gaccattaca cggggtgcat ccgcttcttc atgatggcgc
    11581 agaccaaaaa tgccgcccaa accatctaac tgtaactcat ctaccagcgg ttccatatga
    11641 ctttccatat aagcgcgaac atcttgttca aaaccgcttg ttccttgtaa ttctgttaac
    11701 tctttgattc gttgaaatgt tttttcttcc atatgctatt cgtctaaatt tcagactaaa
    11761 ctcctctcag acttatattg atctgcggat aaaatatgat tttttctgca gaccgatcgt
    11821 atttattata gcttattttt tctggtagcg gttatttatt tcaaagaaaa tatgagagtg
    11881 tggtaaaata agcacaagga gtaacaagtg agaagaagga ggtatgtcca catgtatgaa
    11941 gaaagcgaac taagctattt taaaggcggg ttagcagttg gaatcggtct aggattattg
    12001 agcggggtag cttcaacgat ttggtatcat cgcaaaaaag tattgtcagc agaccaagta
    12061 cttgaaaata tcaagtcatc ttttctaaaa gaaggtgcaa tcgaaggttc ttggatctct
    12121 tttgaaaagg aacctattag gaaattcgcc gttcggtcga gtgcttatcg gggaggtctt
    12181 tctcgaatag aagatggcga aactgtttat tatgaatttt tagcagatgc tcatacagga
    12241 accgtattag aaatttcaag aaaaaaaggt gcaacagatt aatacgtcaa aaaagtgaga
    12301 gaacgaatac gcacccacta ttcaaaagag aggtatcatg tccttctttt gtagtgggtg
    12361 gttttttaga tagagctttc caaacatata ttccagtatt ttctttttaa ataaatgata
    12421 tttagcggga aaaactagca tttcctttga ttatgaggta tttttaattt agcttttgtt
    12481 ttctatagtt aaggagagtg aaaaatggaa caacaagaaa aaattgctgt tctgattcct
    12541 tgttacaatg aggaagcaac aatttcaaca gtcattgcag actttaaacg agaattacca
    12601 gaagcagata tttatgtcta tgataataat tcgaccgatc gaacatacga attggccgtt
    12661 caaggtggag caatcgtaaa aaaagaacct cgtcaaggaa aaggaaatgt gatccgccaa
    12721 atgttctttg atatcgatgc cgactattat ttaatggtag atggcgatga tacttatcct
    12781 gcagaagctg tacatggttt gctcgagaaa ttacgttctg gtgaagcaga tatggtcatt
    12841 ggtgatcgcc tatcaaatgg aacctacttt gatgagaata agagaccttt ccacgatttt
    12901 ggaaataacc ttgttagaaa tacaattact agaatgtata aaacaaaaat tcttgatgtc
    12961 atgaccggct atcgaggatt caatcgtatc tttgtcaaaa gttttcctat tatgagttct
    13021 ggttttcaaa tcgaaacgga attgacgatc catgctttag ataaaaaatt caagttcgtt
    13081 gaaatcccaa tcgattatcg tgatcgtcct gaaggcagcg aatcaaaatt gaatacattc
    13141 tctgacggat tcaaagtgat catgatgatt attaaaatgt ttaaagatta taagcctttg
    13201 cttttctttg ggatttggac catcttcttc tttttatttg gattggtcac ggggattcct
    13261 gtgattcgcg aatatatgct gacaagcttt attacacgca tcccttctgc gattttaagt
    13321 actggattga tgactttagc acttctttca ttagtaacag ggttgatttt ggatacagtc
    13381 gtcacgaacg ctaagaaaga atatgaattg aatctatatc atgtttatca tgaataccac
    13441 agtagaaagt aaaaaaagga tcaagactgc aaaaataatt tgcaattctt gatcctcttt
    13501 ttaattaatt atttcgccaa acgatagatg gcatctgcat agattgctgt tgctctgaac
    13561 aagtcatcaa gactcataaa ttcgtttgct tgatgcattg tatcagtata tcctgggaac
    13621 atcgctccat aagcaacgcc acgtttcaac aaacgtccgt aagttccgcc accgatgatt
    13681 tgttcgtagc ctttttctcc agtgtggtct tcatagactt gcaataatgt agccaccaat
    13741 ggatcatcca ttggtacgta atgtggttcc attacgcgag cacctcgagt aactgttgcg
    13801 ccttcttgcc ccactgtttg ttcgatgtca ctttgcaatc cgtctactgt tacacctttt
    13861 ggaaaacgga aattcatatt gatgaagttc gcttcctctg taccattttc ttcaaaagca
    13921 aatagtccag cattcattgt caatttgccc attttttcat cttcataagc gactcctagt
    13981 ttttctccat aaaaatcttc atggacataa gcagctgcta catggataaa ttgtttagca
    14041 gatcctagga attcatagtt atctaagaac aatgccaaga aagaacctgc attgatacca
    14101 gattgtgggc ttgcaccatg tgcgccttta ccaactaatt cgatttttac atacgtattg
    14161 tccgcttcaa tcgttccgct tactggattt gcttcgatga attggtaaaa tgcttcttcc
    14221 atcgcgatag ctgcatctgc acttggtact tgaagtgctg cagttgctgt tcctggcacc
    14281 atattttcgc gaagtccgga aacaaaagat ttcaagacat aatctcctgc attgtcgcct
    14341 ttgaagcgta aagctaaaga aacatttcct ttttcaccat tgatgattgg gaattcagca
    14401 tctggtgaga aaccaaaatc tggttcttct tcatgttcga aataataagc catatcgccc
    14461 cagccgcttt cttcatcgct gccgactacg aagcgaactt tttttgatag tttcaagtca
    14521 agttctttaa tgatcttcat cgcatagtaa gctgccatac ttggaccttt gtcatcgctt
    14581 gatccgcgtg caaagatctt tccatccttg attactggtt cataaggatc tgtgtcccag
    14641 ccatctcctg caggaacgac atccatgtgt ccaaatatcc ctagcgtttc gtctccttcg
    14701 ccaagatcaa tatgccctgc atagttatcg acatttttta ctttaaatcc atcacgttcg
    14761 ccataagcta acatatgttt caatgcgtcg cgaggtccag gtccaaatgg tgcatctggt
    14821 gttacttttg agtcatcgcg ctcgctgttg acacgtaaaa gatttttcaa gtcttcaagc
    14881 aagtcttctt tacgtgcttc tacttctttt tgccaatcaa ttgtcataaa gttaatcctc
    14941 cacttattta agtcttcgtg ttcatgatac cacaaattac agaaaaaaat agttcaaaaa
    15001 atctgacaat tccttttctt atctgttttt ctctaaaaaa aatatacgct ataatgagta
    15061 aaaggaggtt tttagatgct gataaacgaa caactagtga aaagagtcat cacacctcgt
    15121 ccaaaaattt cacataaagg aacatttgga cgtatcgttt tgattggcgg caatgaacaa
    15181 tatggcggag caatcattat gagtgcttta gctgccgtac acagcggagc gggtctcacg
    15241 actgtagtaa ctgatgaaaa aaatcggagt gctcttcaca gtcatctgcc agaagcaatg
    15301 attgtagttt ggacggatac tgaccagttt ctttccgtaa taaagcaagc agatgtactc
    15361 ttgattggtc ccggattggg tacttcttct aagagtttgg aacgtttcaa acaagtacta
    15421 gaaaaccaat caaaagaaca atggctggtg attgatggct ctgctttaag tttacttgct
    15481 gctaatcatc ttttgctacc ctatcccgaa caaactgtac ttacacctca tcaaatggag
    15541 tggcaacggt tgagttctct accgatacaa gaacaacggg ataaagaaaa caaaaagaag
    15601 caacaggaaa tcggtgctgt gctcgttttg aaaagtcatg agacaaaaat ttatggtcac
    15661 cagcacttct acaccaatcc tttaggttct gctgctatgg caacgggcgg catgggtgac
    15721 actttagctg ggatgatcac aggattttta gcccagtttc ctcaaaatga ccaaacgatt
    15781 gcagcagcta tttatcttca tagtttcatt ggagaccagc tggcaaaaga acaatatatt
    15841 gttttgccga cacaaatcag cgaagcactt ccgaaatgga tgaattattt ttgtaaaaaa
    15901 tcaatggaaa acgaggcatg agtcatggag aaatttacag gttatgtgcg agaacctttt
    15961 tattatgaaa cagatcaaat ggggattatt catcacagca attatatccg ctggtttgaa
    16021 gaggctcggg tagccttatt ggtacatcta gggtttccct ataaaaaaat cgaagaatct
    16081 gggatcatca tccccgttct ggaagtaaat tgcagctata aagaaatgat ccattacgga
    16141 gaaaaagtga gcatccatcc aactatccaa aagtatacgg ggacacggtt ggattttagc
    16201 tacgaaattg tgggaactga agacaaaaaa ttaaaaacta ctggcagtag taaacactgt
    16261 tttttacttt ctgattcgca acgattagtg aagctgagca aagtcaatcc ggagttagat
    16321 cagttattcc aagcgtacgc ttctttggat gatgtaaatt cttaataaag accgtctaag
    16381 catttttctt gtattgtgtc gagtagttcg tataatgaaa agaaaaaagc aggtgatccc
    16441 catgtatgtc gttaaagtaa tgcacggtta catcgataaa actggctgtc gcactcggga
    16501 aaaaaactta gacaatctgc tcattttcaa agacaaaaaa gaatccgaag cttttgctaa
    16561 aagaatcggc ggtcgtgtca aaccgatcca agaggtacga ccagattgat tcaatatttt
    16621 tcttttcgag gctctaatag gcatgcctat cagggctttt tttgcagact gtgtttttat
    16681 gcccacacga tttgtaagcg atttatgtta taatagatta tatctgcata gaaaggaagg
    16741 ttaaatggtt atcaataatt tgcaacagca agcttatgaa tctatacgta aacaaatcat
    16801 ttattccgag ttagcacctg gaagtaaaat ctccgataaa gtgttagaag aacaactagc
    16861 tatcgggcgt acaccgattc gagaagcact gatccaactg cgcaatcaag agcttgtaaa
    16921 aaccattcca cagtccggca cttatgtttc aaaaatcgat atccgatctg cttcattagc
    16981 ccgttatacg cgggaaaagt tggaaaaacc gatcctgcaa gaatgcagtg cgaaaatgac
    17041 caagcaagat caagctaaac tagaaaaaat actagaacaa accgatcagg cagtaatcgc
    17101 tcaaaataaa aaagcttttt ttgatttgga tactgatttt caccgaactt gttatgaaat
    17161 tgctggaaaa cgagaaattt gggattggct agaaagttat agtacgcact tgaaccgttt
    17221 cagatggtta cgtttgacga tttcagaatt agactggggt cgagtattag atgaacatca
    17281 gacaatgctt caatcaatga ttgatcataa ctttgatgaa gtcggatttc tttgcacgat
    17341 gcatttacat atgattatcg aggagcaaga atacgtgatt cataattacc ccgattattt
    17401 tgaagatatt caggacagac ccatcaaata agaggttttg gaatcagcgt tttgacctga
    17461 gtattagaga aagaaacggc aaaatagttc ctcatatttt gccgtttttt ttgagctaat
    17521 accgagggtc agctgattgc acaccgttta ttgagaggtt gtgaaaaagc tgccctgcat
    17581 caagtaataa gaacagccaa gtaaaaatag cttcttatgt ttttaacttg gctggactta
    17641 ttaccgcaga tgcaagcttt tgaacaccgt tttccaagag gtcgtgagcc tgccgttcat
    17701 ctccgagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgttga atattttgac
    17761 ttgtgaccga ggagttgaca ggagaacacc gtttattcgg aaaaaaagga ctgtgacacg
    17821 acttttgtca cagtctcttt atttgtaatc taaatcatac tccgtacatc ttttttctat
    17881 tttttttatt tcttctaaga tcccttggct acgaatttca ttatatggcc aaagtaagac
    17941 tgcctctaat acataaggcg aaaaatcgct ttgattcatc tcttttgaaa aactcatcgc
    18001 tgctatctcg ctcaatgcac gaatgtttga ttgatgtgta taaaaaggca accgccacag
    18061 cacaattttt tcgctttgcg tataaattcc cttaacagaa gcttcgatgt ggtgaaatag
    18121 ttcatgtcca ataatcaaag cttgcgctgt ttctttagag atcgaagcta gtcctgaaca
    18181 ttttaatttt tctaacggct ccgtcatcaa tcgaatccct tcatcttctg cataagttgc
    18241 aaaagtgatt cgttcgttta tttgctctga ttcacaggaa acgattggaa cagccatttg
    18301 ttcacaaatt tctcctacat cggcaaaagg gtactttttt ttgatgctac atgccgtttc
    18361 gataccacaa tcgattgatt gttgtataag aattttctgc ttggacaaag gaatcttttt
    18421 ccaaaataaa tctcgccgaa actggtagaa tccccaaatt tcttcgggga tctgttgtaa
    18481 ttcgtttagt aactcttcta acttatgttt gtttgatatt tccggcaacg acgatcactt
    18541 cttcctccgg ttccatagta gatacttcca gatccatcaa aagctgtagt tgttccagat
    18601 ccgtcgctgt aaaatcagag actcttgctc ctaagcagag ataaaattct ggtcgggcaa
    18661 tcaattcagt ttgataaact gccatatctt cccaaagttg ttcgacagct gtcatataat
    18721 tggctgcagt tgttttcata catgaagcat gtccacgttg gaccttgata aatccttttt
    18781 gatcgatgat ccgaagactt ccagcatttt ttgaacgatt ctgttcgccg catacataaa
    18841 agaaaggtgt attttccaat aagcaaactt catttttatt taatcgcata tcctctgcag
    18901 caagctctag tgcttcttct gttgtgatct ccttggttag atcggttgct ttcacttccg
    18961 tagaaccagt agcgatagcc gttacttttg aagtctgtgg atcgatttct acgtggactt
    19021 cgatcgattc tggtgtagcg cctgattcaa tcgctttatt catcgcttca tttttcaacg
    19081 atcgtatatc ttctttactt ggcgaaggga tgatacgttc tacaacatct cggaccattg
    19141 caagtgctac accaatagaa gaaatcactt cggcgttttc aggaatcgaa tatttcacac
    19201 ccattttatt actaaagtaa gtaatcaatg aggcagcgcc gccccctaca cctacaagag
    19261 aaatctgatc tttctccaag tgatacttgt ctgccaattc taaaatcacc ggttcaatct
    19321 tagcatagga tttttccatg atctgttctg cgatgtcttc aaccgtcgta tgacaataat
    19381 cagctaatgc ttggatggct tttcgtgcag atgggacatt tccataagaa aaatgttctt
    19441 cttgcactaa tccaaggaca ttggctgcac atgtatttgt aatagtcact tcttctccgt
    19501 cttccatcac taccttgacg taatcagcag ggtctccttc tttaggagag aaaaattcaa
    19561 ccttaggacc tttgattttt tctgtctccg taaatactgc ataatctaga ccggcaatat
    19621 gtgcagaacg tggaccgaca tcgatgattc ctgactgatt agcccgtacc atcgaaccgc
    19681 ctgccacacc tagaacccga acatctaatg aagagatata tgttgggtgg ccacctacaa
    19741 ttgaataatc gattgctgga cgtccatttt taatgacccc gatattggtt gttgttccgc
    19801 ctacttcaaa ataaacacca ttagacgctc tcaagtacat caaagagccc atgactgatg
    19861 ctgctggacc ggaaagcatg gttaaaacag gtcgtttttt catttcattg atttccatta
    19921 ctccgccgtc tccgcgcata atcatcaaag aaacattgac ccctgcttct cgtacggaat
    19981 cttctgtaga ggttgccgta tctagcattt ttggtaaaat agatgcattg attgctgctg
    20041 tccgagttct tcttgtcaat ccatataatt ttgtgatgtc agaagccatc gttgtaggga
    20101 tcgattttac cgatgctgct tcataaacaa ctcgttctgg ttccccatta tctactccaa
    20161 atgccataga agaaaccaag acctccgctc tttctctttc taaagaagaa atcgtttctg
    20221 aaacacggtc tacatttaag tgtttgacag gtaagaatgc attgacaatc tctatttttt
    20281 tcttattccc taaatcgata tcgttcaatc tagtttgtcg ttttgctaaa aaaccttcta
    20341 atccaccttt tgccatcccg attacgccga cttttgctac gtctccttca atcagtgcat
    20401 tagtcgcttg tgtggtagaa tgtgcaacaa acacaacatc ttctggactg atattatttt
    20461 ccagcaaaca attttgaaaa gactgaacga cacctgcggc tactccacgg acatcatcgt
    20521 gggttgtttt cactgaagat tttccgatga tttcgtgcgt cgcattatcg attgcgactg
    20581 ctttcgtatg tgtaccgcca acatcaattc ccattcgcac aaaacgtttc attttctttt
    20641 cctccattca tctaatcgtt tttatcgacg ttttgtttac taggcgttcc cgtacaataa
    20701 gaacgtgatc aggcataaga tcgctccgat gatccaacca gttgggatgg atttcttcat
    20761 atattctcga gaagttactt ttgtataacc taatccccaa gcaacccaag attgtgtaat
    20821 atctaaatgc tgaggagcaa tcgttgtgat tgcaaataac gggaacaaaa aggctggcga
    20881 catcgttgga ttaacagcta atacgacagc taaaatagct gagcctgaac cgactagatt
    20941 cattggaccg cggaaaaatc ctagtggtgt caatatagca aatacgattg ccaagactaa
    21001 tggtgattgc ggtatccaat ctccaaaaat agctgaaaaa taaggagaag catacgcagc
    21061 tgaattattg aacatcgcta aggtcatcaa gaaaccaacc attggcgcaa catctactgc
    21121 gccatctgta aataatttcg caaactgacg acagatttct ttataacctc cgcgcaattt
    21181 tccagtaaac agcaatgccc agatacctgc tagaataaaa cctaagatga ttggaagatc
    21241 taaaagaacg acccctaaaa ctggtaataa tacggctaac catgagatca ttggtgcgtc
    21301 gccaccttca gctggtacgt ttgctgccat tgcatagcgt ttctttttgt tcattgatat
    21361 attagcgacc gttagtacca caactaagct tacaatcatc ccaatcatac caatttggaa
    21421 atagtcatta taagtatagc tttcagccgc tggattgaaa ccagcataaa tcgtttgata
    21481 ttgtttgaaa ttgacgatat tagcaaagat tccagccata atcgagccca taaatgagaa
    21541 cagtgcaacc ggtactgaaa tacctaatga catcaagatt ggcaacacaa taactgcaat
    21601 agaaatcact ggtccgattc ctgtcatcga catgaatata acagcggtta caatacatag
    21661 taagctcatc gtgattcttg gcttgtcccc tccaagttca actacctttc gaatcagggt
    21721 agctgcgatt ccgctatcta ctagaacacg cccgaaaaag gcaccaaaga agacatttac
    21781 caagatcgat ttggcgtact cagctggccc cgtcgtatag atatgcgtca acacatcaat
    21841 tactccttgc ccttccatcg ccgggtttgg tgaaaacgtg tttccgacta gcgctaatcc
    21901 catccataca gtagccatga tggcaaagcc gaccatcaag ttgtagcctt ttacacagta
    21961 ccagaccatg aaaaagaatg agataactaa caaaatacca ataataatat cactcatgtt
    22021 attcctccgt ccattttgtt tttctgataa gcaaactaat atatcagtca aactcattct
    22081 aacttatgta gttagcgctt tcattaaagg gtgaccataa atgttcagtt ttcaattaaa
    22141 aaaccttaat tatgctattt tagacttata cgttattgtc ctgtttcatt tgatggagga
    22201 ttgtaaataa aaaaagagga aagaacataa cttttcgtta tcttctttcc tctttataat
    22261 tgtttagctt atttagccaa agcttctttt gcttggtcag ccaatgcagt aaatgctgct
    22321 gcatcgttaa cagccaagtc agctaacatt ttacggttga tgtcgatttc agcaactttc
    22381 aaaccgtgca ttaattttga gtagctcaag ccattcatac gagctgctgc gttgatacga
    22441 gcaatccaca atttacggaa gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg
    22501 tatgaattca ttacttgttc ttttgctgtt ttaaataaag tgtgttttga accgtagtaa
    22561 ccttttgcta attttagcac tcttttacga cgtttacgag ttactgttcc acctttaaca
    22621 cgtgccatgt ataattcctc ctaataaaaa taaatcttca gattctgtta tttgtacttc
    22681 ttatttcatt tttgctagtt gttggcgaat acgtttgaag tcgccactag aaaccatgct
    22741 tgctttacgc aattgacggc gttgtttttt tgttttgccg tggaaacggt gacttgtaaa
    22801 cgcgcggaag cgtttcaatc cgcctttacc agtacgtttg aaacgttttg ctgatccgcg
    22861 gtgtgttttt tgttttggca tgactatatt tcctcctcaa aatctttcgt tatccgacta
    22921 tcggctgtcc aacagccagt tagtcattca aattacttac tgtcgttttt cggtgccagc
    22981 gtcaagaaca tgctgcgtcc gtccattttc gctttttgtt ccactgttgc aatatccgca
    23041 gtttcttcag ctaagcgatt caagactttt tgaccaattt ctttatgggt aatggcacgg
    23101 cctttgaaac ggatagaagc tttcacttta tcgccctttt ccaagaactt acgtgcatta
    23161 cgaagttttg tattgaagtc attcaagtcg atcgtaggac ttaagcgaac ttcttttaca
    23221 ttgatcactt tttgtttttt acgcgcttcg cggtctttct tttgttgttc gaaacggtat
    23281 ttaccgtagt ccatgattcg cgcaactggt ggtttcgcac caggggcaac taacacaaga
    23341 tccaagtttg cttgttctgc aatattcaat gcttctgctt ttgtcttaac tcctaattgc
    23401 tcaccatctg aaccgatcaa tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata
    23461 tcctttgcta tggtcattca cctccaaata ttatgagaga aagaaaagct taccgaataa
    23521 acggcaataa aaaaacgagt tctcttacag aacccgcgcg tacttcacct aatagaacca
    23581 gtgaaaacta tctagcccag tgacattatc aactaaggcg agaagcgggt gcttctgctt
    23641 ttatttctca accatcatag tatagcaaaa actaatcttt ttgtcaatgt ttttcataca
    23701 gtttgtcttc cttttctgaa aatgattaga ttagcagact ttctagcagt gattcccgct
    23761 tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga acctattcca
    23821 ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga gatcttctct
    23881 ggtcttctga tcaacggggg tgaccaaagc tatctcgatg ccaaaaatat ggtattaaga
    23941 gagagtcata tccaaaaact tacgatgcaa aaaacagttt tacgtcattt tgaatgcagt
    24001 aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg cagtttccac
    24061 caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag ttatcttcga
    24121 gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac aaacttgaaa
    24181 gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt cacttggaaa
    24241 aacctttttt tgtctgacaa tcagttgacc ggttcgaact ggtttcgaac ttctctgaag
    24301 ggattagact ttacgactaa tttcttcaca gcaatcgctt tatctcaaga ttctttggcg
    24361 ggactaatcg tcaaccaaga gcaagcactt gtgatcgccc aagcccttgg tttgcagatc
    24421 aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata gataagacca
    24481 aactgatgat caaggcgatt ccccatccga aaacggaatg ggtaaagggt aactggagat
    24541 tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac cagaccgtca
    24601 aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg ccagagagct
    24661 ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct tcgatcagcg
    24721 catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc aatgccttga
    24781 tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc aaaccgatct
    24841 ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat ttcagattaa
    24901 gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa
    24961 acaatgtacg aaacaataaa ctgtatacac tcatgtctgg cattcgttcc aaaagcgatc
    25021 gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt
    25081 ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc tggtgcggaa
    25141 catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat
    25201 ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct tttaagtttt
    25261 taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct tgattaaaat
    25321 agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa gaaaaaagtc
    25381 gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggct actttggttt
    25441 ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt gcttgaggat
    25501 cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct ttgtattctt
    25561 cataccattg atcgtaacgt ctgttcaatt ctttgtaata atcgtataga cttggatcaa
    25621 aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctccaacatc gtatcaaaag
    25681 atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag ggaagttctt
    25741 ccatcatgtt ttccagcagt tcatcataca cacgaacttc tgtttctgtt gctcttccta
    25801 gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc aatacattat
    25861 tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa taaatttgca
    25921 gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca ttatcatcaa
    25981 tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt aaacttgatt
    26041 ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag ctcacttcat
    26101 cactaaatat tgtgtttaac tccaaccttt ttaaatatac ccccaatatc tagtagtgtc
    26161 aattgtattt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg
    26221 ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag taaaataaag
    26281 gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt
    26341 ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt agattatggc
    26401 aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc acgcggttgt
    26461 ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg cattccttga
    26521 agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa atttagccga
    26581 agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa aacagcatga
    26641 catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac gtggaatggg
    26701 atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact tagcatttcc
    26761 tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg cccacggtaa
    26821 tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt ttacaaaagg
    26881 gcatcctttc gactactttt ctttgaacat cgatgctttt ttgattgtcg ctgatacagg
    26941 aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg aaacccagcc
    27001 tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag caaaacaagc
    27061 aatcattgaa aattcaccag aaacgttggc acagacaatg gatgaatcac aatcacttct
    27121 ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag caaaagatac
    27181 cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga ttgcattagc
    27241 acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg gtgctgctga
    27301 aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca cgcgcatata
    27361 ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc cttccaatga
    27421 acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc atcttttctg
    27481 atagctatat ggtagatgaa ttttatctag atggcacctt gcaagacgaa aaagcaacaa
    27541 aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca ctaaaagctt
    27601 cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct gccagcgggc
    27661 tagctgcttt agcaggcgct tgcaatactg ctcttaagct tggattagat gatctctctc
    27721 tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc ggtggtttcg
    27781 tcgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc ccttccgatt
    27841 ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt
    27901 ccagcagaaa tgggatgcgt cggacagtcg aaacatccag tttttatcaa ggctggttag
    27961 attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa gatttccaac
    28021 ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact ttagctgccc
    28081 agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg gtgcgacaat
    28141 tgagaaagca aggcattcca tgctacttta cgatggatgc tggccctaat gtcaaagttt
    28201 tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta tttagcaaag
    28261 aacaagtaat tactgcacat gctggtccag gaattgcgat tattgaataa agggagattt
    28321 ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat gcagttgttg
    28381 aaacaggcca tccagcagtt atcgctgcag tcgatcaatt cgtgacagtc actgtagaat
    28441 ctgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct gtacgttgga
    28501 caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat tatatccttg
    28561 ctgctattcg cttgactgaa aagtatgcac aagaaaaaaa cattctttta tccttttatg
    28621 atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga ttgggttcta
    28681 gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg ttgaatttat
    28741 ctcagttgga gattttcaag attgcagcac tagccaattt agcagttcaa gataatggtt
    28801 cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca accttcgacc
    28861 atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt gccctggatt
    28921 ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta ttgattggtt
    28981 ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt tcgagagaag
    29041 ataaaatggt ggcttatcag cttttcttaa aaaatagtac agaatgtgtc aacgaaatga
    29101 tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga aaaaaccgac
    29161 aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca
    29221 aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt gcaggtgggg
    29281 gcgattgcgg aatcgtaatt gttgaccaga aatctggcat tcttccttta atgagtgcat
    29341 gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa
    29401 accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg ataaacaaaa
    29461 aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag cagtttctga
    29521 tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt atatcaatgc
    29581 gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag
    29641 agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag tcccttcttt
    29701 agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca ttgccaacat
    29761 cggtgcaggg acttctgtcg aaagagccaa agaagctatc cgattgtttc acgcagatgc
    29821 cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag accgtgattt
    29881 taccaattgg aaagtactta ttcaagaaac tcaaacagcc attgacgtcc ccctcatcgt
    29941 aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg ctttaggagt
    30001 tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg
    30061 tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag tctcttcatt
    30121 gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag gtatccgcaa
    30181 tgcctatgac atatttaaag ccctttgtct cggcgcaaat gccgtgggta cttcaggaac
    30241 cgtactgacg catctgatga atcacggcgt agaagaaact attatactga tgaagcaatg
    30301 gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg cggccctaca
    30361 ccaacaatct ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa gagggatcga
    30421 cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa gaacactttt
    30481 gtctcacgct ctaaacacgt acaaacggcg gaagtaaaag caactccttc ggaaataagc
    30541 tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt cttggagtta
    30601 aatgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct gtccttcggt
    30661 attcgttcaa tccgctgatt tcacaacctc tccactaaac agtgtactaa agctctttta
    30721 aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat ggccgttaac
    30781 gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga ttggctcgcc
    30841 ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac gttcaccgtt
    30901 ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg gtccaattcc
    30961 attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca gcatttttaa
    31021 tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac tcatcatgga
    31081 gcagatattg atgattttac cgccgccttt ttcgatcata tccggaataa ctgcttttga
    31141 aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg ccgcatccat
    31201 ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat caactgaacc
    31261 tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca
    31321 gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt catcaactga
    31381 ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag agcgtgcaat
    31441 ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat ctaaacgaaa
    31501 catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat tgatccattt
    31561 gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag atgaatgagt
    31621 aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg gcttgttcat
    31681 tgtctacaac taaatgtttt gtttcatctg gtttgcccat catgtggaac acacgtgtat
    31741 cttcattggc ataatcaaag tagacataag cttccatacg acgttcatgt gtatgacaag
    31801 gcatcgtatt ccaagcgctt cctggttcta aaatcgtata acccatttgt aattgacagc
    31861 tttcgcatac atttggatgg atgtattggt agattttacg ttcattcagt gtcaggcctt
    31921 caccagtctc cattggtttt acttggtcga tactgatttt tacatttgga tatttatgat
    31981 gagctggaac agaactaata tagaatttag ctgggttttc agcatcttga gaagaaaata
    32041 caacatgtcg tgtttctttt ccgatatagt atccgtcttg ttttttcatt tcttcttttt
    32101 gcccatcgat ctcgatgaat cctggtccac cgatattgat cacacctagt tcacgacgct
    32161 ctaagaagta atcaacacct agttctttgt ctaatgtgat ttctaattct ttttcagtag
    32221 gtgtcactcc accaaagatc attcgatcgt tgtgcgtata agtcaaactg attttccctg
    32281 gttcaaatac cttttctact aaaaattctt ttctcaattg ttccgttgaa taatgacgga
    32341 tatcttctgg actgtgtgta taacgtgttt ccatttcttg cattttcttc tactccttct
    32401 atcgaatgat ttttccagag ccgcatgcta aaaattgtgc gacttcttct tgactaaatt
    32461 ggttgcagtc cccatggatc gtatgcttca aggcagaagc tgccgtagca aaatcaatga
    32521 tttcttgcgg agacatattg gataaaatcc catggagcac gccacctgca aaagcatctc
    32581 cgccacctac tcgatccacg attggggtga ttctatggac ttgggattcc acatattcat
    32641 ggttcatcca caatgtcccc ttcaattcat tttcacttgc tgaatgaacg gtacgcttgg
    32701 tcgaataaaa cacctgaata ttcgggaact tttcctgcat tttttgataa taataaatta
    32761 gaggttgttc ttctgcttct ggcgctttct cgatacctaa caaataaaga gcatccattt
    32821 ttccagctga gcagatatct actaaaggta aaagctgatg gattacttcg ctggcttctt
    32881 gctgtgtcca taattttcca cgatagttga tatcgaagct gatcaggcag ccagcttttt
    32941 ttgcagcttc tatcacctgt ttcgtcattt tttgccattt tttagacaaa gctggggtga
    33001 ttcctgaaac atgaaatata tctattcctt ggaataactc ctctagcgac cattccaaat
    33061 gatccatttc agcaaaactt gatccggcac gatcatagat cactgaggcg gcgcgttcgc
    33121 cactaccagt ttccatataa taagtaccta gtcttgatcc accagataat acctgtttcg
    33181 tatctactcc ataacattgc aagtgctttt tgactgcttt tcccaatgcg ttatcaggta
    33241 cttttgtagc aaaaatcgct tcatgtccgt aattagctaa agaaattgct acgtttgctt
    33301 cccctccgcc ataatgggca gcgaatcgtt cacttgaatc gatacgggtt ccctgctctg
    33361 tggacaatcg caacatgatt tctccgagtg tcactacttt tcccataagc agctacctct
    33421 tgatttcttt catttttgct gcatactgtt gcgcgacttc cgttactttg tcaaaatctc
    33481 ctgtagctgc gggtgctaaa aggttcccac ctactccgac tgttacgaca ccagcatcga
    33541 accactcttt catattgtcc agactcacac cgccagttgg catgatattg acttgaggca
    33601 tcggcgcctt gaaggcagaa atgatacttg gtccataagc gcttccagga aatagtttga
    33661 cgatatctac cccgcttttc agcgctgttt tgatctcggt aatagtcata catcctggta
    33721 agtaaggaat ttgatacaga ttgcacatct ctgccgtttc ttgatcaaag gaagggctaa
    33781 caatatactc tgcgccagct aagatagcca agcgagctgt gacagcgtct aatactgtgc
    33841 ctgcgccaat cactatttct ggttggtctt tgtagaatga caaaagttct ttgattactt
    33901 gatctgcttg agggactgta aaagtcaact caataccagt tagaccacct ttgatgattg
    33961 cgtggcaagc attcaaagct tcttctttgc tttttccgcg taccaccgca atcactccgg
    34021 cgtttttcaa tcgagataaa atatcaattt ttttcatctt ctccacctat ttcctattta
    34081 gaaattttat tttataactt ggaaatctat acccatatta tacggcagaa aagaaaaatt
    34141 gcaaacgctt ttttcattat ttttgaattc tttgctcgat ttcgcttttt gtttgcttga
    34201 gggcactgat tatttgttct tttgtctcat ctgttaaacg atatttcggc atacttacac
    34261 taaatgcacc aatgatttgt tcgtttttca cgattgaggc tcctatacaa aaaatatcac
    34321 gttcgatttc ttcatcgtca aatgcgatgt gggtttcttt taccgttttg atttcctttt
    34381 tcaaccgtaa tgcattggtg atcgtatatt ctgtgtaagg gatcaacgga tgcttttcaa
    34441 gataatcagt gtactgctct tcactgaatt ctgctaatac ggctttgccc atcgcagaat
    34501 tgtataatgg tcgggtgatt ccgatttttg aagacatgcg gatcgtttga tgttttggat
    34561 ctagtttgtt cacgtacaag atctcattgt tgtccaatac ccctaagtgg atagtctcat
    34621 cgatcttttg ctgcagctgt tctaaaaacg gtaaagtcaa ctctaccaaa tcgatttgtt
    34681 caatgttttt attcgcgtag cgaataagct ttgcacctaa acgatagttt ttctctgaat
    34741 ttttatttac gtagccgatc atcaacaaag tatctagtat tttcaagact gtagaagagg
    34801 tcattcccac accctttgcg atcgtctgca aagagacatc ttggttttcc gctaaatagt
    34861 ctagtatttt agaggctttg atcaatacag ttccataagg ctttttattt tccatataat
    34921 attctcctat ttctttataa gaaaaattat agcggaaata ccatcaattt aaaagagata
    34981 tccagatata aactaccatt tctctttaca ggcaatttaa tgttgaagat ccgcttcgtt
    35041 tttacatcaa actttgatat aaagcaatga tttcttctac tgtcgtttct cttggatttc
    35101 ctggtgtgca tacatctcgc aaagcatctt ctgcaattgc tggaatatct gcttctttta
    35161 cacctaattc agaaatggtt tctgggattc caaccgcttt gctcaaccga tcgatctcac
    35221 cacaagccgc ttctcttaca tcttctaaag acatttcagc tgctccttta atccctagaa
    35281 caagagcgat ctcacggtac ttttctccag tatattcttt gttatatttc atgacagttg
    35341 gcaaaagtgc tgcacacgct acgccatgag gaatgttgta ccatgcactt aacgggtgag
    35401 ccatcccgtg taccagtcct aaaccgacat ttgagaagcc cattcccgca atatactgtc
    35461 ctaatgccat tttttctcgc ccattttgat cgccatctac agaagcttcc aaagaatggc
    35521 cgatgatttc gattgctttt aaatgcagca tatcagtcat ttcccaagcg ccttttgtga
    35581 taaacccttc aatagcatgc gtcatagcat ccatacctgt tgatgcagcc aattttttcg
    35641 gcattcccat catcatatcg ctgtcgatga acgcgacgat tgggatatca tgaggatcaa
    35701 cacaaacaaa ttttcggtga tttacttcat ctgtgatgac gtagttgatc gttacttctg
    35761 ctgctgttcc agaagtcgtt ggtatagcta atataggtaa acatggattt ttcgtatcag
    35821 caacaccttc taaactaatg acgtctgaaa actctggatt cgtcaaaata atcccaatgg
    35881 cttttgccgt atcgattggc gaacctccac cgattgcgat caaacagtct gcctctgcct
    35941 ctttaacaaa agccactcca tcttttacat tttggatcgt tggattaggt acgatcccgt
    36001 cgtataaagc ataagcgatt gctgctttat ctagaagttc tgttactttt gttgctatac
    36061 cgtgctcgat caatcctttg tcagtaatga cagctgcttt gccgaatcct cgctgctgaa
    36121 attcagggac gatcgactca atcgctcctt ttccaaaata cgatgtttca tttaaaatca
    36181 ttcgattact cattttctat tcctcctaat ttgtattatt tttcacaata aaaacgaaag
    36241 aaccccaagc aaccggttgc ccagttgctt gttgctacag ttcaaagact ggcatcaatt
    36301 cccaactgac tggagaggca tctggatttg tctccatgat atcttccatg tacttccacc
    36361 atttttgatt gatagatgta tttgccattt tttcccataa cttttcatct tcgatcacta
    36421 agtaaccaaa taacaaactt gtactcggat caagatagat cgaatagtcg atgaccccgt
    36481 gttcacgaag tgctgtacgc atttctggcc aaagttcatc atgtcttttt ttgtattctt
    36541 cttgtttttc tgggtaaact ttcatgcaaa aagctttttt caccatgtca gacacctgct
    36601 ttcgtatgaa cttcctccaa ataacctgct tttggagtga caccaaacgc atctgctaaa
    36661 tgccacaggt tttcgtccgt gattgtttgc aagattggtc cttgcgcttt gacgtacgta
    36721 tagacttctg ctgccttttc tgctgtctcg atcaaaccaa agacttcatc catgtctttt
    36781 cctgacccgt agattccatg ttgtggccat aaaaccaatc gagtttcttt cattttttca
    36841 gctgttgctt ccccaatctc atttgttccc ggaaccatcc acggaatgat ggaaattcct
    36901 tctggaaaaa caactaagca ttccgtacac atttgccata gcgtacgact aaaggaacgt
    36961 tcgtctaatt catgcgtaaa actcatcgct aatagatgac tagcatggca atgcataacg
    37021 atccgattat ctgggtctac ttctaaacgg gcgatatgac tcatgaaatg acttggcaac
    37081 tcgcttgtag gaaggcattc gttttccagc ccccacaaaa gttccagtgt tttgccatct
    37141 tcagcaactc gaacgattcc aagattctcg gcaggttcgc tcgctacatt tttaaaatat
    37201 cttcctgaac cagttacaat aaaatagcat cctgctaaac ggctggcatc aaaaatcatc
    37261 gagatgcggc ggatcacttt ttcaggatcc aagaacggtg tgatctcttc ttcttttagt
    37321 aaataagaaa tattcccgcc atttctttca tcccatccca agcgataaag attcgacgtc
    37381 gtttcgatca tttctttcac ataaggcgct tgtaaaatat ctattttttt caatatagcc
    37441 acactcctat tttcttaatt ccaacacgtc tttttgatat tcctggactt cttccagcca
    37501 atccaaacca actggaactt gatttttgta acagaaataa ttccatacat cggcatacgg
    37561 gaaatctttc aattcttccg tataagccat tcttgaagta aagtcaaaag ccagctccat
    37621 ttctttcaat cgctcaactg gttccagcat agctttcagc agtgcttttt gtgtatttct
    37681 cgtaccgatg acccaagcag cgatacggtt gatcgttgca tcaaagaaat ccagaccgat
    37741 atttgttttt tccaacaatt cgttacggac taattcttta gcgatttcct gcaattcatc
    37801 atccatcaaa accacatggt ctgaatccca acgcactgga cgggaaacat gcaacatgat
    37861 tcctttgctg aataaagcta atgaagataa tttattggaa atcacttctg ttggatgaaa
    37921 atgtccagca tctagacaaa caagtttgtc acgagtcaac ccgtaaccca tataaaattc
    37981 atgggaaccg actgtataag cttcagcgcc taaaccaaat agtttgcttt ctactgcatc
    38041 ttgtacatac cgttcatcta actcttctgt aaaaatttca tcgagtgatt ccattaaacg
    38101 ttcccgtggg gtaaaacgat caatcggatt atctttcatc ccatcaggta cccaaaagtt
    38161 attgatactt gtttgcccag tttctttccc aaagtactcc gcgattttgc ggcttctttt
    38221 cccgtgttcg atccagtatt cacgaacttc tttatctgga tgagccaatg taaagccgtc
    38281 tttcatcatt ggatgcgaaa agaaagtcgg attgaagtca agaccgattt cttctttttt
    38341 tgcccactct acccattttt caaaatgttt cggctcaatt tcatttagat cgacagattc
    38401 ttctgtatcc aaatagatag catgaaggtt caatttgtgt ttgcccggta tcaacgaata
    38461 agccttctcc agatcttgtc taagctcttc aggtgttcgt gcggctcctg gaaaattccc
    38521 tgtcgccatg attccaccgg tcaattcgcc atccggtgtc aagaaacctt tgacatcatc
    38581 tccttgccag caatgcatag aaattttgat gtcctgtatc ttttctagta cagcatctgt
    38641 atctacaccg atagttgcat atttttcttt tgcttgttcg taatttcttt caatcgtgtt
    38701 catacgatta ctcctttgat tttattcacg taccattcca tcgtgttttg cttattttca
    38761 tctgggtaaa aagtttcaca tggaaacgac tgtttgatta catttcttgc ttgttctata
    38821 ttcgaaaact gacctgtact gagcatttgc atggcaacat tcccgattgc tgtggcttca
    38881 ctaggtcctg cttctacaat gattccggca taatctgctg tcaattgatt cagtagacga
    38941 ttattcgatc cgccccctac gatgtgcagc ttttccaatt tcgttccagt taagtgttct
    39001 actttgatca gctcttgtgc gtaacaaaga gcaagattat catacacaca acgagcgata
    39061 gctcccgatg agacagggac tggctgctct gtttcaaaac agtattgctg aatctcttct
    39121 atcatatttg ggggattcaa aaatcgaggg tcattgattt ccatcacttg ctgcagcggt
    39181 ttttcttttg cagcaagctc tgccaattcc gcataactgt actcgtactc ttgcattcgg
    39241 gcaacttcct ggatcaacca catccccatg atgtttttca aaaaacgcac cgtgttatgg
    39301 gcaccccatt cattcgtata attttcagaa aaagcattgc gagaaacatt ggcgatcttt
    39361 gtttcaatcc ctaaaagcga ccatgtccca ctgctgatat aaccccaacg ttctccttga
    39421 cctggcgttc ctaatacagc tgacgccgta tcatgacttg ctactgtgat aaaggtcaca
    39481 tctggcaaat catattttga aaattgttct ttttgcagat ctcctagtat cgttcccggc
    39541 tctgtcaatg gtggaaataa ttccttcgag acaccgattt tttcaagaag ttctatttcc
    39601 cattcttttg tccctgcatt cagcagctgc atagtagatg cattagtttt ctccgtcacc
    39661 atgttaccag taaagacata ccctaaataa tcaggtatca gtaacaattt atgtgctttt
    39721 gccaaatttt ctttttcttc agcaaacaat tggaacaagg tattgaaagg ctggatctga
    39781 atccctgtct gctcatacaa cttatctaga gaatagattt cagaaaactg ttctactgct
    39841 ccattggtac gcttgtcccg ataagcaatc ggatcattta tccgatcccc ttgttcatca
    39901 atcagacagt aatcgactgc ccatgtatcg atccccgcaa aaaggcgatc aatccctgtc
    39961 tgctttaatt tttcaaatcc aatcaaaatc tctttgataa gataatcgat cttccagcga
    40021 tcgtgaccgt ctttgttccg aaagccgttt ttgaaccggt gtacttcttc caagatcaaa
    40081 gaaccttttg catccctcgt acttttcatt agtcgtccgc ttgatgcacc gatatcgatg
    40141 gcaacaaatg ttggattcat ttctccacct cttttacttt attgttgatt agcactttat
    40201 taataaagcg ttatcattta ttgacaatgc tatcataata cagaatctta tattttgctt
    40261 cacactattt tggtatataa ttgcattatc ttgacaagtt gggagtgttt acatggatta
    40321 ctgggaaaaa gccaaccact catggtcgga agactcaaaa agagtgatct tgacgcctac
    40381 acaaagaagc aggaatttat ttttttatat ccaagaaatc ggttacttca aagcaccaat
    40441 cccttactat actgaacgag cacatttacc ttcttacttg ataaagttta ctttgtctgg
    40501 caaaggttcg cttacatata atgataatca gcaacagcta aaagctggag acttattttt
    40561 tattgactgc caagaatatc agcgttatca aacgatcagc gaagaaccat gggaaatgga
    40621 ttggatccat ttgaatggtc agaacgtcca atcattttat caggagttca caaaagacgg
    40681 ccataacacc ttccattcaa caggagatcc atcagaaaac aagatccacc atttgatcac
    40741 agaattgatc cacatgcaac aaggcacgaa tgcccgtacc gatttccagt cctctgtagc
    40801 tatccacgag ttgctgaatg aactgatttt gcaaaagtat cagctagatt tcgaagaaga
    40861 ggatattccc gcctacgttt acaaactgaa gcaatactta gacgaacatt tcaaagaaac
    40921 gattacactt gatcaattag aacaacaatt cttattgaat aaatatcagc tgagcaaaga
    40981 tttttccaaa tttatcggtt ctccaccaat cgattatttg atcagcaaaa aactttctta
    41041 cgctaaagac cttcttcggt atacagaatt tacggttcga atgatttccg aagaaatagg
    41101 gattgagaat tttgcttact ttagccgcct gttcaaagca aaaacaggac tatcgccgag
    41161 agactttcgt aaattcagct gattcatata atatgtacgt cattttttca aaatattttg
    41221 aaatatatag ataaaaataa aataaccgca gtttctgctt ctatgtaaac aacagcagct
    41281 actgcggtta ttttttagtg atcattctca ttgttcatct tcaaaatgtt cataatgaac
    41341 caactgtttc tgtgtatcac gtttcaaaaa ttctttaggc gttatgccga actctctctt
    41401 gaatgctttc agaaaatgcg gataagaaga aaaaccactt ttactatata cagcactaga
    41461 gctgcggtat tctcgcaaca agtgcttggc atagatcagc tttttcttca ttacatactg
    41521 atgaaacgta aatccagtgt actttttaaa ttctcgtgtg atatggtatt tgctgacaaa
    41581 aaactgcttt tccatttcct ctaaagaaag cggatgagat aaattttttg agatatattg
    41641 taacatttgt tccacacgct cattaggaac tacagcattc tgttcttgcg cactctcttc
    41701 tttttgtaca acttgattga gaaaaatcaa gtattctacc agagcttgtt catattttat
    41761 gtcttcaccg taaatttttt tatcaggagg atggtcgata aatgataatt tttgcttcaa
    41821 tacttcctgt tctatttgca atacacgact cctcttctct cccattggtt gaaaacaagc
    41881 cgatagatta gaccatggcg tggaacgact ttctagaaaa gaaggggtga caaagatata
    41941 gacacgctcg aatacatctg aagattggcg gacgatacga tgcaaatcat tggaatggat
    42001 gagcagcaca cttcctgctt tgatcgtaaa ttgtcgtccg ttcaaataaa aagtcgcttc
    42061 tccctttaat gtagcatgga tctcatagaa atcatgatga tgatagatcg ttttattttg
    42121 gctgacgaca tctttgacat gatagatttc atactgatca gaaaccatcg tattgacttg
    42181 ttcaccgctg attttctttt ggagcatgtg ccgcacctcc ttttgttcac ttatgcacaa
    42241 ttttattata ccacgttttt tgtaatcaaa aagagctgct tttatatact cttataaaaa
    42301 agaagagacg tggcatcttc tagccccgcc ccctccattc taaatattag ttaaccaacg
    42361 tcttctttac attattcaag aagacttctt ccataacgaa tcccatcatc ttttgtttct
    42421 tcaagaacca catacaggta cggacgattc ttcatgatga tttctttgat ttttgtgtac
    42481 tcgatcacac cgtctcccaa atttaccggc acgatttttt cctgttcgac acgaaaatct
    42541 ttcaaatgaa ccgcacatat cttttcacca aaacgttcaa aagcttcttc taccaattgg
    42601 acttgttctt gataatcact acttgtgatc aaattggtcg gatctaaaat gattcctaga
    42661 tagtccgaat ctactgcttg gatcagctgc tctacccttg ctaaagaata caccggatga
    42721 ttcaaacctg gttctatccc caccatcatt tggtattttt ctccagcttt taccaagcga
    42781 cggataacgg acacagcttc agcaaatgct tcatctgtga aattttcttc cgtatattgg
    42841 atttccggca aaacacatcc agtttccgaa gcaaccatcg acgcaccaaa ggaagaagca
    42901 tagcgaagat actgctcgaa ctttgtcagc aactcttgtc gtatcgttaa atctggatga
    42961 atcatattga tatagcaact taaaattcct acggtcactt cttgcttttc taaaactcgt
    43021 ttgacataat ttcccatccc agaattgatc tcatttgcgc cagaaggtat ttcaggaaac
    43081 gaaagaccta aggcgagctg cagtgtatga atcccttgtt cttttgtttt ttctgcaaga
    43141 tcttctatcg tttggacttc ggaaagatca tgtcctctaa ttgtaatgtt caacataact
    43201 attctcctaa ctcagcatat tgggtagcca catgacgatt tgcgggaagt aagtaatcaa
    43261 aaacaagacg actaagataa ccccataaaa aggaagaagc ggtttcagca ttgcctctgc
    43321 ttttacgcct cctacacttg caccgacata tagtcctgta cctactggcg gcgtgatgga
    43381 gcctacacac agattcatga ttgaaaacaa cccgtaatgt acaggatcaa cccctacact
    43441 tgtgacgatt ggtaaaaata ttggtgtaaa aatcaagata gcaggcgcca catccataaa
    43501 catacccacc agtaataaca caagattgac taataacaga ataataaatt tattatccgt
    43561 tacgcccata atcaacgaac taattgcctg aggaataccg gtaaacgcca tggcgaaaga
    43621 catcatggaa gaagtagcca ataagaacat gatcgtccct gacattgcta ctgattgaaa
    43681 caacatatta ggtatatctt ttagttttac cgttttatag taaaacatag caatcaacag
    43741 ggagtaaatg acagctatcg ctgaagcttc tactgctgta aatatccccg tcaagatacc
    43801 accgataatg atgacaatca atagaaggct aggaatcgcc tcttttatta tttttccagc
    43861 atgagccatc tgtccttttt cgacaacagg gtatttattc tttttagcaa cgatgaatgc
    43921 tactagcatg attcctagcg cccacaaagc gcctacgaga taacctccca taaacaaagc
    43981 agagatcgaa gcaccaccag caacaagtga atacatgata aaagcagtac ttggaggaat
    44041 gaccatacct gtcggagctg acgcgatatt gaccgctgtc gcaaacttcc ggtcatatcc
    44101 ttcttccact tgctgaggga tcaacactcc gccaatcgcg gtagaagcgg caatggccga
    44161 gctagagatg gaaccaaata aagcattccc taacacattc gtatgagcca aagcccccgg
    44221 cacgcgcccg acgaacaaca tcgcaaaatc cactagtttc ttagctatcc ctccattatt
    44281 catgataatg cccgagagaa cgaaaaacgg aatagcgatc agcgaaaaac tattgatact
    44341 tcccaccatt ttttgagctg aactaaaagc agaaacatca aacggtataa ctaacaataa
    44401 agtcatcatc gatgagaatg caatacttat agcaatcggc atccctaata caagcaacac
    44461 aacaaataca aaaaataaca ctaatccggc ttctattgcc attttattcc tccattttta
    44521 tattttttct tgaattgctg gcttgttgct taatgtatcg attagattga tcaggctata
    44581 gataatgatg agtacccccg atacaggtac tgacagataa acatatccca taggaatact
    44641 taaagaaggc gagatctgcc ccattgtcaa tgtgacagca ttccatccgc catacaccat
    44701 gatcatcgca gcaaatacca gaaataaact ttgaacaaca acttctaaaa ttttactttt
    44761 tttgccagtt aatttttcac ttaaaaccgt tacggctaaa tgtgcttttt gtcccacaac
    44821 atacgcggaa gacaacatgg caaaccagac aagaccgaat cgaacaagct cttccgtgac
    44881 cgtatttgga tttcctaaaa ccgttctgga aaatacttgc cataaaacaa cagcgaccat
    44941 cccaacaagc aaaacagctc ccaggatctc taataccttg tttaaaatca ttttcatcac
    45001 agctacgctc cttgctcttg gatcttttga taaaggtctt tgtaggtagg atcgtttttg
    45061 aatatatcat gaagtggggc aactgcctct cgaaacggtg tattatctac atcattgaat
    45121 tggactccat gttcttcttg tgctaaagct ttttcttctt caattgcttt tgcaaaagct
    45181 tcaatttcaa aaaccgttga ttctttcgct gcttcttcaa tggctttttc ctgctcatca
    45241 gttagctgtt ctttgatggc atcattcatg atgatgatat ctggcacacg tgtatgctca
    45301 tcataggaat agtatttagc cacaccacca tgtccagctg tatacaagac aaattcattg
    45361 ttttctgatc cgttgatcaa attggattga agagacgtgt acacttcatc acttcccata
    45421 ggtacgggcg aaccgcctaa aagctctacc attttgatag cggtttcgct ttgcatcaca
    45481 cggatcttct ttcccttcaa atcttccggc gtatggatcg gaccgtctac catataaaag
    45541 ctgcgctgac cagaatcata ataggtcaat ccggtaaatc ctaaattttt ggtcgattgg
    45601 tagattggct ccattatttc ttcattatcc atcaccttaa agaaatgttc ttcatcattg
    45661 aatagataag gaactgaaaa aatcgaataa tctttagaga aactttccaa cgctgaggca
    45721 cttacttttg tgaaatctac tgctcctgac tgtgtcaatt cgatcagttc acgttctcca
    45781 cccagttgtc catctgggaa atactggatc tgtacggaac catctgtttt ttcttttact
    45841 aaacgcccaa attcattcat tgcatcaatt actggctggc tgttgcttgc ataagcaaag
    45901 cgcaaggtaa ctatctcatt tacatctgca tctgcgacag ttgccacact agaattattt
    45961 ccgcagccgc ttagacctag cagacctagg ctaagcgaac ttgctgcaaa cagcagtgat
    46021 gtttttttca tatgggtttc ctttctttta acaataagag acgagcaatt ctgtcaaaca
    46081 aagcaccgtt aacgactgtc cataaggcat tgctgtgatt ccgatattcc gataaaaatc
    46141 taaactgtcc cccattcctg tcccgattga aacgttttga acttctcctt tttcatcgat
    46201 ttgttcaagc aatccttgga tagcacgata agcgacttct ttatattctt gtggtaaata
    46261 acgtttatgg accgctttca aaataccgta agcaaatcct gcagttgcag aagattctac
    46321 gtaagaagaa ggatcatcca acaacgtatg ccataaaccg ctttcagact gataagtttt
    46381 taatgcacgc acttgtgctt caagtgtttc gatcaataat ttgcgcaatg catcttcttt
    46441 cgataattct aagatttcaa tgatctctgg aatagcaatc gtcaaccagc agtttccacg
    46501 agcccaaaat gcttcggcat agttatgatt tccttcaaac gtccagccgt gataccacaa
    46561 tcctgttcgt ttatccatca agtatttgat atggatcata aactggtaac gagcttcttc
    46621 taaatattct gggcgattca acagtttccc gatttttgcc aaaggaagca ctgtcatcat
    46681 caatgtatcg tcccacaact gattcttatt ttccggtcca tatgtggcat gctgtaaccc
    46741 gtcttcattt gtccgaggca tgtcattcat gacccattct gcccattgtt ctaaataagg
    46801 caaatagcgt gaatctttcg tgtcttcata caagtaagcc atagtcaata gcggtgccat
    46861 tgtattgacg tttttgggcg gagcgccttc ttccatacga tcctcaaacc attcattgac
    46921 cacttgataa gcgcgaggat tttttgtcat ctggtaattt ttataaatac catatagacc
    46981 gacaccttgt ggccaattcc acactgtcca gcttttgtca tctactttca gtccatcaaa
    47041 gtccaacaaa aattcaccat tatcgtcttt gatcgttgtc aggttttcaa cgagcaggtc
    47101 aattttttct ttcactaatt ctttaggtac aaaaatctct tctttaataa tcatctttac
    47161 gccacctttt taaacgcttt cattttatat ccttaatata gcagtaatac cgtccactat
    47221 aaatatcgct ttttgaacaa aaactgtcta aaattgacct aaatgttaaa taacaaaagt
    47281 aactttcagt tacttggaaa ttaaaaaaag aacttggaga aacaacgttt tccaagttct
    47341 ttttcatcta ttttcttatc ttttttctac gctttctttt gcttatccgt tgtaagcatc
    47401 aatgatgatc tgtttcaatt cagagatcaa tggttctttc ggattagctg tcgtacattg
    47461 gtcttcgtaa gctaattcag ccatacggtc tacggttgta tctagtgttt cttgtgtgac
    47521 tccttgagct tttagactca tgttgatgcc gacagatttt cctaattcat aaacagccgt
    47581 tgctaaagct tcgactaatt cctctgttgt ttcgcctttc agacctaaga attttgcgat
    47641 atctgcgtaa tctgtatctg cacggaaata gtcatattta gggaacatcg catgtttttg
    47701 tggatctttt gcattatagc ggataatatg cggtaaaaga atcgcatttg tccgtccatg
    47761 agggatcccg tattccccgc cgattttatg tgcgactgag tgacaaatac ctaggaatgc
    47821 gttagcaaac gccataccag ccattgtaga agcgttgtgc attttttcac gtgattccat
    47881 atcaggtgtt tttactgatt tttctaaata gtcaaagact aacttgattg cttgtaggct
    47941 caatccacgt gtataatctg aagccatgac tgaaacatag gattcgattg catgtgttaa
    48001 cacatccatc ccggtatctg ctgtgacaga tgctggaact gacatgacga attgaggatc
    48061 gacgattgcg acatctggcg tcaatgcata atctgctaac ggatatttca catgtgtttc
    48121 actgtcagtg atgaccgcaa atggcgttac ttcagaacca gtccctgatg ttgtcgggat
    48181 acatacgaat tgtgtttttt ctggtttgtc gatcttatat gtccgtttac ggatatccaa
    48241 gaatttttgt tttgcaccaa agaaagatgt gtctgggtgt tcatagaaca tccacattcc
    48301 cttagctgcg tccattgcag aaccgcctcc aagagcgatt actgtatctg gcttgaagtc
    48361 tgccatcagt ttcgttcctg cgtatacggt atttgtcgat gggttcggtt caacatctga
    48421 gaagacttcg atcttcacat catttttacg tcgtgccaat acttcacgaa ctgtatcgca
    48481 gtaaccgaat tggaccatac cagggtcaca aacgatcatg acacgttcaa cgttttccat
    48541 tttctctaag tacaatagag agttcttttc aaagaagatt tttggcggta atttgaacca
    48601 ttgcatgtta tttctccgtt tcgctacagt tttgacattg attaagttta cagcagagac
    48661 gttacgagaa acagagttct taccataaga accgcatccc aaagtcaatg acggaatcat
    48721 ttcgttatag atatcaccga tgccgccttc tgcagatggt gtattcacta aaatacgaca
    48781 agctttcatt cgaagaccga atttgacatg caactcttcg ttttctgaat ggataactgc
    48841 tgtatgtcct aatcctccaa gattcaacat accttcacac aaatcaaacg catggtccgt
    48901 gttgttagct ttgatcatgg caagaactgg agaaagtttt tctcttgata gcggataatc
    48961 tgggcctacg ccttccaatt cagcaatcag cattttcgtt ccttttggta cactgattcc
    49021 tgccaatttt gcgatttcca tcgcagaatg tccaacgatt gatggattga ccgcatattt
    49081 tccttcgttc atcactgcat cttctaattt ttgcaattca tctggtttta cgatataaac
    49141 ttggtgtgct tggaattctg cttttactgc tgcatagatt tctttatcca cgatcacagc
    49201 ttgttcagaa gcacaaatca ttccattatc aaatgttttc gaaacgatca aatcgttcac
    49261 tgcacgttta atttttgctg ttttttcaat ataagctggt acgttacctg gacctacacc
    49321 taaagctggt tttcctgtag agtaagcaga cttgaccatt ccagctccac ctgtcgctaa
    49381 gacgatcgcg atacctggat ggttcattag catagaagtc gcttcgatcg aaggatgttc
    49441 gatccattgg atacaatttt caggagctcc ggcagcgata gcagcatcac gtaccactcg
    49501 tgctgcttct gcagaagatt tttgggcact tggatggaaa gcaaaaacga ttggatttct
    49561 tgttttcaac gcgatcattg atttgaaaat cgttgtggaa gtcggattcg ttgttggagt
    49621 tactccgcaa accacaccga ctggttcagc aatttcaatc aacccttttt ggacatcttc
    49681 attgatcaca ccaactgttt tatcatgttt gatattatgc caaatatatt cggacgcata
    49741 catatttttg attgctttat cttcgtaaac accgcggcct gtttcctcta cagccatctt
    49801 cgctaatggc atatgttgat ccaaagctgc cattgccatt tcatgcacga tatggtctac
    49861 tttttcttga tcaaagcttt ccatttcttt caatgctgca tttgcttttt ctgccaaatc
    49921 atcaatcatt gcttgtacgt caattgtctt cgttttttct ttttctatta ctttagccat
    49981 cattatcctc ctaatgggtc atggttattt gttaaataat tcacatgtat atcatacaat
    50041 atttattttt ctatgtaaac acttttttgt gatttttttc acagttattt ttaaatataa
    50101 aattgcttta tttacaggtg tttgtataga aaaatatata aagttattta ttattattta
    50161 tatgacacac ccgattattc aatattgagc aatatatccc atgctcttat ctctttcttt
    50221 tttgaataaa aaatgaggta ggacatcttt ctgccctacc tcgtctttaa ataagctttg
    50281 atacttattt ttcttccttc tcttctgtct cttccagcgt ttctgttgtt tcagttttta
    50341 cttcaactgt ctcagctggt tcttctgttg tttcaacagt agtcgttgca tcatttgtta
    50401 cgacagtccc tggtttgacc gttttgattg ctgcacgatc gtactcaagg aagatccctt
    50461 cgcagtctaa aacaactgtc cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc
    50521 cgccgatcgt tacgactgaa tcacctggtt tcatgctatc taacaagttt tgacgttctt
    50581 gctgttgttt cttttgcgaa cgtgacataa aaaaccacat tgctaatagc gcgataaaca
    50641 tcacaagcat tggtaatgtt cccatttttt tcacctcact aattctgtat ctacaaaaaa
    50701 cactttagca gaaaaatgct gataacacta gttttttact taatttttaa aagattttcc
    50761 tttagaagct ttttgcgttt tctttattga acccgtattc ttcaaaaaag gcttctctaa
    50821 attcaagcaa attatcatcc atgattgctt gacgtacttg tttcattaga ttcaacaaga
    50881 agtataaatt gtggtaagaa gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat
    50941 ggcggatata ggcacgtgta tagtttttac atgtataaca atcacatttc tcatcaattg
    51001 gacggaaatc atgggcatat ttcgcatttt tcactactag acggccttga gaagtcatac
    51061 atgtgccatt tcgagcgatt cgagtaggca agacacagtc aaacatgtct actccacgga
    51121 taacaccatc gatcaatgaa tcagcagcac cgacacccat caagtaacgt ggtttgtttt
    51181 cggggatcaa cggtgtagtg aattctaata cacgattcat ttctgattta ggttccccga
    51241 cagataaccc gccgattgag taaccaggga aatccatccc gactagatct ttcgcgcttt
    51301 gacgacgaag atcttcaaac cctgctcctt ggatgattcc aaataatcct tgacggtctg
    51361 gattagcatg agctttcaat cctcgttctg cccatcgaga cgtacgttct actgattttt
    51421 tcacgtagtc gtaactttca tcgaatggtg gacattcgtc aaaactcatc atgatatcag
    51481 agcccaattt attttggatg ttgattgctt tttctggtga taaaaacata cgcgaaccgt
    51541 tcaagtgatt tttgaaatgc acgccttctt cttcgatatt ccgcatatca cttaatgaaa
    51601 aaacttgaaa gccgcctgaa tctgttaaaa tcggttgatc ccagttcata aatttatgca
    51661 gtccgccagc ttcttctact aagtcttcac cagggcgtag ccataaatga tatgtattac
    51721 ttaaaataac gccagcaccc atttctttca actcttctgg ggacattgtt tttactgtag
    51781 ctaatgttcc tactggcatg aacattggtg tggggaaagt tccgtgtggc gtgatcaatt
    51841 cccctaaacg cgcgccagta tgtttttctt tcttgattaa acgatagcga atggcaggtt
    51901 gcgtcattca tttccctact ttctatataa aatatcttga caactcccac catcataatc
    51961 gtttcaagtg ctttttgcaa gttccctcgg cttccttcta ctttatttaa tattcttttt
    52021 ttatcctttt tttatgaata acaaaaaaaa gaaaaacatt atgacagaaa tattttttga
    52081 tacatggtat attggtgtta ttaaagaaaa ggagggaaaa atcatgcaat cttctgcaga
    52141 attaaaacgc caagcaaaag agagtctaaa aggacgttgg ggacaagctg ttttactaaa
    52201 tctaatccct acattgattg gtattgctac tattcttctt attatgattc ctattatctt
    52261 gtttgccacc accatatatg atggatcagc gagcgtctca gaagcgacaa cttcaggagg
    52321 cggtagttca ggaggcggtg gcggaattat ttccagcatc attagcgctt tgttcatgag
    52381 cggtatttct tggacttatc ttgatatttt acgtggacaa aaagatcaga tcgaaccatt
    52441 caaagatgca tttcgtggat tcaaaggttt gtttatcggc ggagtgatca gcttagctgt
    52501 gctgatctcg atctttacaa cattatgggg tttgctatta gttattcctg gaatcattaa
    52561 aagctattct tattctcaaa gctattttat ttactacgat atcgtccaag aaacaggaca
    52621 aacacctaaa gtactggata cgatcactgc aagtcgaaaa ttgatggatg gttataaagg
    52681 acaacttttc tggttagatc tttcatttat cggctggcat atcttagcat tagcaactgc
    52741 tggtattggt tatctatggc tcagcccata catttctgca acaaaagcag cattttatga
    52801 acaattgcca aaagatattt aaaagagagt gtgacttgtg accgaggggg tggcaggaga
    52861 acaccgttta tcgagaggca gtaaaagaaa cattcagccc attagcaaaa aggaggtgtg
    52921 acaacattca tgtcacacct cctttttgta tagttccagt aaaaagaaaa tcaacttaga
    52981 aacagttaat cattcctctt cttctgcttc cggtaatcga tggaacttga tcagttcatt
    53041 caccgcgatt ggtacgatag ataaaatccc taccaagaac cagtgattca aactgatgtg
    53101 tactaggccg aatagttctt gcgtaaatgg cagcaaagca acagccgttg taatagctaa
    53161 tgccagtaac gccatttcta ccaaagcttt attggtcgct aaattgactt tgaagatcga
    53221 ttggctgctg cgtacattga acacatgcag aatcgatgaa taagctaaga tcaagaatgc
    53281 aacagtttga ccgacatgct gacttgcgtc tacccatggg ctgatagtat cgacataact
    53341 acctaaataa taaccaaaca aggttacgat ggtgaagacc cctgcattta tggcaatttt
    53401 ttgcaataaa cctcgagcaa agatcccttc atttttcgga atgggtggct gatccatgat
    53461 gccgtgttcc gctggctctc ttcctaaagc aaatcctgga agtccatcag caactacatt
    53521 gacaaacagt agttgcacag ctgtaaatgg tgctccccat cctaacagca tcgcaatcaa
    53581 aacaataaag atctccgaaa tattacagct tagcaaaaag ttgatagctt tacgaatatt
    53641 ctgataaacc gaacgtcctt gactgacagc gtctacgatc gtcgcaaagt tgtcatccgt
    53701 caaaatcata tctgcggcac tttgcgccac atctgttcct gtgatcccca tggcacaccc
    53761 aacgttactt gctttcaacg ctggtgcatc attcacacca tccccagtca tcgctacgac
    53821 tgcacccgta cgttgccaag atttgacgat ccggatcttg tcttctggcg ttacccgagc
    53881 atagacagag agagctttga cacgcgcatc taattcttcg tctgataatt gatgcagttc
    53941 tgatcctgaa agtgcttctg acggatcatt caagatacct aattctttcg ctatggcact
    54001 ggcagtcacc acatgatctc cagtgatcat gactgttttg atgccagcct ttttcgctcg
    54061 cttgattgct cctttacttt ctggacgtgg tggatcgatc attccgatca gaccaatcaa
    54121 acgtaagtct ttttccagtg cttcagaaga aatatcttgc ggttcttcgt cataagtagc
    54181 ataaccaaca gcaatcactc tcagtgcccg tttaccaaag cgatcattaa cgatcgccgc
    54241 ttgttctaca tcgcctgaac taaagcgcgg agcaagaaca tcgaatgctc ctttggtgat
    54301 tgagatgtat ttttcgccca tttggtgaac tgttgtcatc atttttcgtt cagaatcaaa
    54361 aggaagctcg tttactcgtg gatatttttc ttctaattct gcctttgtat gataattctc
    54421 ttcaacagcc cttacgatgg ctacttctgt tggattacct ttgataacaa ggtcgtcatc
    54481 ttcttgctcg accgtcacat ctgtacatag tgcagccatt ttcaatattt ccatcgcgct
    54541 gttcgtcatt gaatcttcga tattcgttac ttcatcttct ttgctccata cccggcggac
    54601 gcgcatttca ttttgtgtca gcgttcctgt tttgtctgag cagataacat tagcggttcc
    54661 tagcgtttca actgctggca actgtcggat gatcgcatgt ttcctcgcca tcttctgcac
    54721 accgtatgca agtgtcaacg taacaataac agtcaaggtt tccggtacag ctgcaacagc
    54781 caaggaaatc gaagtcatga acatctcaag gagtggttct ccttgcagtt caccaatcac
    54841 aaatactaaa gccgcagccg ctaaagcgat catactgatt cgtttgccta gctgattcaa
    54901 tcggacctgt aaaggtgttt tttgcatagt attttcgttc aaaagatccg cgatcttacc
    54961 catttctgta gccatgccaa tggcagtgac gatcgctttc ccacgacctg cagcaaccgt
    55021 acatccttta aataccatat taagttggtc acctaatgac tcatcttctt cagcaataaa
    55081 tgctgcatct ttttcaactg cttcactttc tccagtcaac gccgattctt ctacacgtaa
    55141 ttgtgatgct tcgatcaaac gggcatcggc agaaatagca tcaccggact ctaaaacaag
    55201 aatatcaccc actaccagtt gctctgactc aacagtggtc actttgccgt ctcgtatgac
    55261 agttgccgtt tgtttattca tatcttgcaa ggattccagt gctttttctg cgtttccttc
    55321 ttggacaatc gccaataccg agttgatcac aacgatagcg atgatcagca gtccttcaaa
    55381 taaatcccca tgttctgtag caaatgcggt ataaaaggaa atcaccgctg ctacaagaag
    55441 gatgatcgtt gtaaaatcgg acaagcttcg aaagaatttt ttgatcatcg attctttcgg
    55501 cgcctcatcg aatttattca atccctgctt ttttcgccgt tcatccactt cttgtgaaga
    55561 caacccagta tctggatctg cttctgttac cttctttact tcttctattg tctgcttgta
    55621 ttcttccatc ctattcctcc tttaaaatct tcgtacaaaa aaaggtactt attttactat
    55681 aacatccttt ttttatttac ttcaaataaa gggcttatcg attatttgtt aagaaagcgt
    55741 aaaatttaaa gtgcttatat ttgcaaacgc taccaaagga tgctattata tagatgtaag
    55801 gaaggaggat accatcaaat agacagaaga atttctcgat agaacagtgt aagcaaacaa
    55861 agttgttaga agtacaggtt tcacccagac atgcgtgtag agtacaaacc tataaatagt
    55921 gaatgcgtat ggatgtttgg atcacgaagt atccgaaaca agaagtaatg cgatacgtaa
    55981 caattgaata acgtaaacgt ctgaaaattg aataggaaca agagatgttt caaatgttga
    56041 agatgtaacg aaaacaaaat ttttgtttaa catgaagaaa ttcaacaccg tgctttgcat
    56101 gtatgatgga aagttacaat catttctgtt tatttgaatc cttccgaggt acaagaaagc
    56161 cgagcttatc gagagctcgg ctttttcttt gtcataaaat ctttgtaaag cttttttgtt
    56221 attcaaaact agcggctttt cataacctta tttcacgaac atcgcatctc caaaactaaa
    56281 gaaacgatag cgctcttcaa tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc
    56341 aaaggcacta actaacatga ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc
    56401 ttctaccact ttgaattcat agccaggcgt aatgaaaata tcagtccatc cgctgtctgc
    56461 tttgatttct ccattaaatt tcgttccaat cgtttctaat gttcgaattg aagttgttcc
    56521 gacagccacg attttcccgc catttttacg tacttggttc aatcgctcag ccgcttcttc
    56581 cgtcaaacgg tagaattcac tatgcatatg gtgttcttca attttatcta cgctgacagg
    56641 tcggaatgta cctaacccta catgtagtgt cagatagact aattcaacac cctttgcttc
    56701 gatctcatca agtaattctt tcgtaaaatg caatccagct gttggcgctg cagcagaacc
    56761 attttctttc gcatagacag tttgatagcg atctgggtct tccagacgtt ctttgatata
    56821 tggaggcaat ggcatttctc ccaatgattc aagaatctct agaaaaattc cttcatactc
    56881 aaagtcaatg atccgtccgc cgtgttccaa ttcttctttc actactgctt gcaatctgcc
    56941 atcaccaaaa gagattcttg tcccaacttt ggcacgtttc gcaggtttga tcaatgtctc
    57001 ccacgtatct ccttgtgtat tcgttagaag aaggacttct aaatgcgcac cagtatctgg
    57061 tttttctcca tataaacgag caggcagaac tcgtgtatta ttcataacta gcgcatcacc
    57121 aggctgtaac tcttcaatga tttcatggaa atgtttatct tccatctctc cagttttccg
    57181 atccaaaaca agtaaacgag aatgatcgcg gtcttttaat ggtgtttgtg cgattaattc
    57241 ttccggcaag tcaaaatcaa aatcttctgt tgttaacata tcgtcactca ctttctatca
    57301 tgtgcttcat cattctatca tgtttttcgt tctgttggca aaatcagcac tttttcactt
    57361 ctattttttg ttttcctaaa taaaaaaact accctatttg tattttttca ttgttttagg
    57421 catactagta atagatagtt cttcattgaa aatagaaaaa gaggtggcac catgattgaa
    57481 tttcaaaatg tgtcaaaaat ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt
    57541 tcatttgaaa agggtgagtt catttgtttt atcggtacga gcggaagcgg aaaaacgacg
    57601 acgatgcgga tgatcaaccg aatgactgat ccaacacaag ggaaaatttt gattgatgga
    57661 aaatccatcc aagaaattga tccagtggaa ttgcgacgtc agatcggcta tgttatccaa
    57721 agtattggat tgatgcctca catgacgatc cgtgaaaata tcactcttgt ccaaaaattg
    57781 ttgaaagtca gtcaagaaga acggaataaa acagctgaaa aaatgattga tcttgtggaa
    57841 ttgccccgtg agatgttaga tcgctaccca catgaattat ctggtggtca gcagcagcgt
    57901 attggagtcg ttcgtgccct tgcagcgaat caagatatca tcttgatgga cgagccattc
    57961 ggtgcgcttg atccaatcac tcgtgattcc ttgcaagacc ttgtcaaaga tttacaagag
    58021 cgtttgggaa aaacgatcgt atttgtaacc catgacatgg atgaagcgat taaactagca
    58081 agccgtatcg ccatcatgag tgaaggccgt gtgatccaat ttgatacacc agaaaatatt
    58141 ttacgtcatc ctgcaaacga tttcgtagaa gagttgattg gagaagatcg cctactacaa
    58201 gccaagccaa atgcgacaag agtcggcgaa gttatgctaa atacagcaat cacgattaca
    58261 ccggaaaaat cattacaaga agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg
    58321 ttagttaccg ataattcgaa tgtattaaaa ggatttatcg atgtagaaac gttgaataaa
    58381 aaacgaggaa aagtatccag tgtcggcgac attttgaata aacatgtttt ttacgtaaaa
    58441 gaatccgcct atcttcgtga taccttgcaa cgcattttaa aacgcgggct gaaatacgtg
    58501 ccggtcgttg atgaacagaa caaagtcgta ggtatcctga ctcgtgcatc attagtggat
    58561 atcgtctacg atgtgatttg gggagaagat cccgcaacta ctgatgttgc agagtcagct
    58621 actcctgaag aatctaagga ggtctgacct atgcagcagt tttttcatca gcacggttca
    58681 gaaattctct caaaaggttt tgaacatctt tatatttcag gacttgcttt gatcattggt
    58741 atcatcattg ctgtaccgct tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt
    58801 gtgattggct taaccagtgc cttgcagaca atcccttctc ttgctttatt ggcattgatg
    58861 atcccgctat ttggcgtagg taaattacct gcgattatcg cactctttat ttattcatta
    58921 ttgccgattt tacgtaatac gtacattggg atgaaaaatg tcgattggaa ttatcgggat
    58981 gtggcaaaag gcatggggat gactgagttc caatccattt tttccgttga attgcccatt
    59041 gctatgccaa cgatcatggc tggtattcgt ttagctgccg tttacgtgat tgcatgggct
    59101 acacttgcct cttatatcgg cgctggtggt ttaggcgatt tgatttttag tggactgaat
    59161 aattaccaac cagatctcat tttggcaggt acgatccctg taacaatttt agcactattg
    59221 gctgattggc ttctaggtct cttggaacac cggctgacac cgatcgcact tagagaggag
    59281 aatgaagaat gaaacgtata ctaaaaatga ccatggttct tttctctctt cttatacttg
    59341 cgagttgttc cttccctggt cttgccagca atacagatga agacaccatt tcgatcacag
    59401 gtggaatcac ttcagaagca cagatcttag caagtctagt tgccggaatg gttgaacatt
    59461 atacagataa aaataccgca atcatcaaca atctggctac tacaactatc aaccatcaag
    59521 cgatgatgaa cggagatgct tcaatttcag cggctcgcta cacaggaaca gatttaacga
    59581 ctacattgaa ccttcctcct gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg
    59641 aatttgagaa aagatacggt caaacgtggt tcccgtccta tggttttgaa aatacgtatg
    59701 tctttcttgt tcgaaaagac accgcacaaa aataccatct atctaaagtc agcgatctga
    59761 aaaatgtagc ggatgaatta gtagctggtg tagatacttc ttggatcaat cgtaaaggag
    59821 acggttatga cggcttccaa gaaacttatg gcttctcctt caattcgata ttacctatgc
    59881 agattggact tgtttatgac gcagtcgaag caggaaaaat ggatattgtt cttggctact
    59941 ctacggatgg acggatcgcc agttatgatc tagtcatgtt ggaagatgat cgtcaattct
    60001 tcccaccata tgatgctgct ccagttgtag acaacaagat attagctgat acgcctcatt
    60061 taaaagaagc cttaaataag ttaggtcata ccatcagtac ggaaaaaatg caggaattga
    60121 actacgaagc agataataat ctagtggaac catcagtagt agcacaacgt ttcttacaag
    60181 aaaaccacta ttttgaaggg aagtgaagca attggataaa atgaatgtct ttcaacaatt
    60241 tttctattat ttccaggaaa atggctccta tatctttgca caatttatcc gtcacttcct
    60301 gatttctatc tatggtgtcc tctttgcagc agttgtcggg attccggttg ggatcatgat
    60361 ttctaggaga cgtaaattag caaactgggt gattcgtttg gcgaatatca tccagacgat
    60421 cccttctcta gccatgatct ccattttaat cattggatta ggcctgggag tcaatgtcgt
    60481 gattgttact gtatttttgt attccctctt accgatcatc aagaatacgt ataccgggat
    60541 gatccaagta gacaaaaaca tccttgatgt cggcaaaggc atggggatga ctgctagaca
    60601 acgactattc atggtagagt taccactctc tgtttctgtt atcatggcag gcataagaaa
    60661 cgcgttagtt gttgccatcg ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct
    60721 tggagatatc attatccgag gaaccaatgc aacagagggc acatcgatta ttttagcagg
    60781 tgcattgcca actgcattaa tggcgatcat cacggactgg cttttaggta ttttggaaag
    60841 acggctagat ccagccagcc gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct
    60901 gcgaccattt gttggtcgca aaggcttttt tagttctgtc atttttaagg caataatcca
    60961 ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg
    61021 tatactccaa cttcatactg ccaacgggtt catatctgca tccaccgact aagttttttt
    61081 cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc
    61141 acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct
    61201 ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct
    61261 gcatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg
    61321 aacttgccat cctcatcctt ggctaaaaac atatctagcc attccagctt ctcgccatgg
    61381 gaggtttgcg gttcctaaaa ccggtacacg attcttatgg actgcatccg tcgcaactag
    61441 acttggcgat acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat
    61501 attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt
    61561 tgatactgct attcatggcc gaaagcgcca cgactttaac ttcttccgtt tgagtgacat
    61621 tcatgttcgg caatgtcgct ttggataccc gctctgctaa aggaacgaca ctggtattgt
    61681 atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag
    61741 acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa
    61801 cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca
    61861 acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt
    61921 ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag
    61981 atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa
    62041 aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc
    62101 acttcactca taatgtgtcc aggcagaata aatttctaca actttatttt cctcatcaac
    62161 tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg
    62221 tctcagcttt tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac
    62281 ttcttcaaat tgtttcttta aattcgtttg cttgattttt cttaaatcca ctttcgcaga
    62341 gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata
    62401 ttcgtaaagc tactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc
    62461 tcaagaaaaa gggtttcttg aatcgctctc cagtcttcca gcccaatcaa tacagcattg
    62521 tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat
    62581 aactgataaa aatttttacg agcagtgcta ggattcatcg ttgtattagc cattgtctct
    62641 cctccttttt ctgatataat tatagcgtac ttgattaagt acgtcaaaaa aatacaataa
    62701 agcacttaaa ttcagcaaac ttttcctctt ttttacatag agaaaaagac ccctttttag
    62761 ggaggatctt tgtccacgct cacttcttac tcctttacga ctagcggtag ccaaacgtaa
    62821 agtaattggt aacaacgtat catctttagc aaaggtcgaa tccaaagcct gtgagtctat
    62881 gcaacaacac caatacacga aggttcctcg ttgcagtgtg acttgattga tatcttgaat
    62941 aacctgagga tacgcacgaa agatttgtaa cggttattca aaactgattt tggccgtcaa
    63001 gtcttctact gcctcaaaaa tgattgcctc tttcgtctcc tgtcattcat gttcactatc
    63061 gatttgatac ttgcttagac cgttgggttt gtgaatcacc aattaaaaat ggcactgagt
    63121 tatggtaaca aaaaatggtc acttgacgag gctgagattc ctacttatga agacacatgg
    63181 cacttaagag tcgaggtaaa ggaagaagct gaaaatatag gaggctgtac attatttggc
    63241 aaaggattcg gaaattatga tcaagacatc aaaatcaaaa tgttttacag ctaaatagga
    63301 agaataataa catttcatac gctgttccca tctttaatgg cttatagagg gcacctttag
    63361 ctgagaataa gacaaaacag accctctagc ttcaattaag agctagaggg tctgtttttg
    63421 attttaaaac ttggtttatt tcttcaaatg gtagctatag gtgctgacaa cgatattttc
    63481 acgccaagac aatcgcaatc ttaaccgcaa acttgtttgg ttatggagaa tattctgcca
    63541 gcgtctgttt ggtacaaatt gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc
    63601 atttttactt acgatatcta cataacggat aattggattt tcaatcgagc gataagaaga
    63661 gtggacgatt gaaaaacgga catctgggaa atgcttttta aattctgctt cgatttcttt
    63721 ttctttttcg acatcttcgt ctagcgaaac atgcatcgca acgacataat caccgatcga
    63781 acgtgcgtaa ttcaacgcac cgatatttac ccgtgtcaca ttcccgacta atacaagaac
    63841 ggtattcccg tcataatcat gtagttttgc atcattttct aaacgaagct gttctgctac
    63901 ttttttataa tgatcatgga tcttataaaa aatgaacata acgattggca taatgatgaa
    63961 gaaaggccaa atgtccccta accgatagac aaacaaaatc gcaatgattg catatgagat
    64021 gaacgcaccc gtaatattgg caatcgattt gcttaaccat ctttttcctt cttttttcca
    64081 tttgacgacc atccctgttt gagataaagc aaatggaatg aacacgccga tagagtataa
    64141 cggaatcaag cgttctgttg agccatggaa aataaacagc aatacgatcg aacctagcgc
    64201 tagtgtgata atcccattgg aatagcctag tcgatcccca cgatcttgat acatgtgagg
    64261 catgaattta tcttttgcca gattgtatgc aagaactgga aaagcagaaa aaccggtgtt
    64321 agcagcaact gctaaaatca gcgctgtagc aaattgaagg acgtaataca aaataccatg
    64381 tccaaagaca gctttaccaa tttgagacaa aacagtgact tctttttcag gaacgatccc
    64441 ataccaatag ttgataaatg tgatccccac gaagaagaaa cctaagatca atgccatcat
    64501 cgtcaatgta gcagcggcat tttttgctct tggcttctta aagaatggta cagcattact
    64561 aatcgcctcc accccagtta aagaggaaga accagaagaa aatgctcgta aaatcaaagc
    64621 gatagaaaca cccggcacag ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc
    64681 tgtcacgata ttatataaac caacaacgat cagtaacgag atcacaagga taaatgtgta
    64741 caccggaaat aataggaacg atgcagattc cctcagccca cgaagattca gcatcatcag
    64801 tagcaaaacg attgtcacag aaatagcgac ttgatgaccg tagagtgctg gcaccgctga
    64861 tgtgatcgct tcagcacctg ctgagacaga tactgctact gtcaacatgt agtcgatcaa
    64921 taaagagcca ccggaaatca agccagcatt ctttccaaga ttctcactgc tgacgacata
    64981 tgctcccccg ccatgaggat aagcatggat gatttgacga tatgatagcg ttaatgaaat
    65041 caataaaata ataacaaaag cggcaatggg tagcgaatac cagatggctg cagcagacaa
    65101 agcgactaaa acgacaacga tttgttccgt accatatgca atagatgata acgcatcaga
    65161 cgacaataat gccaaagcag caaatcgggt taatttgtgc tcatcatttt cagcagattt
    65221 taaaggctta cctactaaca acctttttaa gtaatccaat ttctaaactc ctttacacac
    65281 aattttactt caagttttat agtcgaaaat ttatagtaaa caagatgtta tagtacgctc
    65341 actgacctta aaagtcaata aactttaaga aaataattgg aaaaaaacag aaaaatatca
    65401 agcgcttttt caaacgcttt aatacagcaa aaatctaaca tttttttatg ggctttttct
    65461 ttgaattcta taataaaagg atagaacttg acttagaaaa tcaagttcta tcctttttga
    65521 tatcttaact tttggaagag agatgtaagg ctgctttcta cccatcattc ttactactcg
    65581 aagcatagcg cagttgtcac aacctcttga taaacggtgt ttaaaagctt gcatctgcgg
    65641 taataagctc aaccaaatta aaaacataag aagctgtttt tatttggtcg ttcttattac
    65701 ttgatgcaga gcagcttttt tacaacctct tggtacacgg tgttcaatca gctgaccctc
    65761 ggtattagct caaaaaaacg gcaaaatatg aggaactatt tttccatttt tttctctaat
    65821 actcaggtca aaacactgat ttcacaacct ctttttttaa tgaaaaatat ggctctcatg
    65881 cgccgcttct ttgaatttcg tcattttttc ttcgatgaac ggatagattg ccgttcctac
    65941 gattccaaag acaacaaaca aaccgatcat gatccagatt gctttcgtag catttggcca
    66001 ataaattcct cctgcagatt cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg
    66061 attgatcatt tggaagaatt tccctgagac ttggatcgga tagttcccgc ctccgccaga
    66121 gatcgacagt actaagatga taatagcgat ccctttaccg acattatcga ataatgcagc
    66181 caagacatag acgatcatca tgaacgctaa ggcgatcaag acagcaaatg acacgctgta
    66241 gaccggattt tggacatcta ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct
    66301 ttgcgcaatc gccattgtca aaaatgtcag catacgagca acgaattgtt ctcgtttcgt
    66361 aaaattgttt cggtctttct ttcctaaata gtattctgtc gtagcaacac ttgaaagaag
    66421 aacagcgccg acccacagac atagtgcagt ataaaacggg gtgctagctg aaccgttatt
    66481 tgcgattgga taaagcgcat tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc
    66541 ttttgttgcg tctagtttca ataatttgat gacttcgcct agatcgactt cttgttcgcc
    66601 ttttcggatc gcgtctgctg ctttttggat accagctttg atatttggcc agtcattgtt
    66661 gatcaagtcc gcagcagcgt tcacagctgt ttcgacttct ggcatttttt catttactgt
    66721 tgtcaatgta ccggtgattt cattttttag tgctggccaa tcattttgga taaaatcagc
    66781 ggccaatcct aatttttgct gaatgatcgg taattcattg ttgtataggt ctgccccttc
    66841 attgatgcca ttgacgatgg tatccatatt tccattcagc attgtattgg catcatggat
    66901 ttcttgtcca attgcaggca tttctttttg atatttttct aaaattgcca ctgcattcgt
    66961 tacagttgct ttcgttgagc tgagaagcga atcaaagtcg atctggtccg ctttgctcag
    67021 tgcgccttgt gcagtcccaa tcgtatcgat gacttggtct agtagagaag tgacagtgga
    67081 actgatttga cctgtattga taccagcaat gatgccattg atattattaa caacggcatc
    67141 aatttgatct agcaattcat caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa
    67201 agcagatagg cggttttgca gattgcttag caagggtttt aattgattta gctgatcaat
    67261 aattggctgt aatttcgtat ttccagcact tgtttgcaat ttagtcagga actcgaccag
    67321 tttatcgatt gcattttgtt gttgagatat tccgtcaata aagccgttca atacattgct
    67381 gatgtttttc ttttcatcat cagtcagctt accatcttca atagctgttc ttacagtatc
    67441 tattaacgaa cctgtcgttg ttgccacttg tccaattgac ttcaatgtaa catctacact
    67501 gtttgtgatg cttggtatcg catctttcag ctgagaggca cctgattttg tttggtttgc
    67561 tagatcactt gcctgatttt ctaaattttt cacatctggc aagatatctt gtacttgttg
    67621 gatgatcgtc aagccttgtt ttgcttcatc gattccttgg ttcattgtct cttggatcga
    67681 agcaaaatcg ccgtcgatct ctgctagttg cttaccggca ttttggatct ctgggatttt
    67741 ttcttgcaaa tcaaggatga tttttgcttg atctttcaac tcaggcattt tgtcattcaa
    67801 tgcaacgact ttttcaccca tctcatctac tttagggatg taatccacga attcatttgc
    67861 tttattcagt ttttccttga tttcaggtag ttgactttgt aattcgagga cttgttttgt
    67921 gtagccgtcg atcgtgtcca aattttcatc tgttgataaa atcatatctt taactttatt
    67981 gatacttact aggttcgtat cgatgtcgta acctatctca ttgaatactt tcaataaagt
    68041 cgaactggct gttttgataa attcatttgt gatttgcgat tggatcgaac ttgctccttt
    68101 atcggtgatt ttcggtgcaa tcgcattgat tttttcattt accgtatatt cgatttttgg
    68161 tttcttgata tcaccactag taaaactcag cagatcttct gagaaatcct ttggcaagta
    68221 aatacctgcg taatactttc cggaacggac tccgtcttct aattgttctt ttgaatcaac
    68281 gaattgccat cctaattgtt tatttttatg aagcgattcg atgacttgct ccccaatggc
    68341 cacttcttta ccttgaaact ctgctggctt gtctgcactg taaacagcaa ttggcagctc
    68401 tccagtattt ccatatggat cccataatgc tttgatgtta aaccacgcat atagcgaagg
    68461 gatgatcatg atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca
    68521 gtcaagcaaa tataatcgaa gcgtattttt gattgactgc atagttctcc cccttcttct
    68581 tttttcagct gaattacttt accatgtatt tcatactttt ttcaatcggc ttttttcaaa
    68641 aaagtcgaat ttttggacat atcacggcaa ttgtctcatt gtaatctctt acatattgag
    68701 aaataattaa ggattacagc ctttacttta tcatgaaata aaagggaaag ataattttat
    68761 gccaaagttt ttatctatta tacgtatacg caaaaaagct aaaacatttt ttgtctttgc
    68821 ccctacaaaa atgtttcagc ttttttcgtt ttttattcct gtatttctta tttttcgaat
    68881 gacaggatga ttttcccagc aggaggatgt ccttcaacta gctgatgtgc ttggatgaca
    68941 tgcttaagtg aagcagacag attcattgaa ataaaaacat gaaaggctcc tttttggtag
    69001 gctttgatta tgccagcaaa ggcttctgca tctagatatt cctttcttgg aacaaacgat
    69061 tcataaaagc cttccttttt ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca
    69121 tacctgccgc ctggtttcat gatctggatg ccggtttctc ctttgatact tcctttcgtc
    69181 gcatcgatta caaggtctgc ctgatcagca aattgcagac ctggatttgt tttatcataa
    69241 gcagcaaatg ctgaagctcc taattttttt accttttcct catttttact ggatgcactg
    69301 gttaagattc ggattccctt ttcatggaga agctggatca gacttgaccc gaccgcacct
    69361 gatgctccta gtatcattac agtatctgtc ggctggattt ccgtcaaatg gttgattaga
    69421 ttataagccg taattcctgg cgtgaccatt ccggctgctt cttcccaact cattttatct
    69481 ggaatttttg ctacttttgc tgatggcaac acgacttctt ctccgtaagt cccgccgact
    69541 gcatggacag ctactcgatc acctacatga acagttgtga catctgaacc aatctctgtc
    69601 acaataccag cgccatcatt acctggtaca taagggaatt tcaatgttcg tatctctttc
    69661 atttctccta aacgcaaggc aacatcataa ggattcacac tgaatgcttt tatttctaca
    69721 cgaacatgtc cagaaccgac ttctctggga tgagcatcga tttcttcaaa aacatcttgt
    69781 gcttctccgt aaccgttaat tgcaaatctt cgcatggtca ccatctccct ttttctttaa
    69841 gtttacgttt atgtctttat tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc
    69901 aaaacaaaat agttgtcttt tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa
    69961 gattacgatt tacgctaata aaaaataatt ggaggaattt gttgatggct aaaaaatcaa
    70021 agattgccaa agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct ttacgccagg
    70081 aattaaaagc gcaaaaagat tacgaaggct tgcgtaaact tcctttagat gctcgtccag
    70141 aacgattgaa aaatcgtgat ttaatcgatg gacgccctcg cggatacatg cgtaaattcg
    70201 ggatgtcccg tataaaattc cgtgaattag cacataaagg tttgattcca ggagtaaaaa
    70261 aagccagctg gtaatccaag tctggctgta atgtaaagga ggagggcgca tggaccactc
    70321 cttggatcat caaaaaagat acgttcgctt cttctagcag aaatgctggt actagaagca
    70381 gacgtatctt tttttacgca cagctggtcc ttttttatat gtaaaggctg catggttact
    70441 tcgctatatt tatttagtaa caggagtcag tgcttcgata cccacactaa ttttcaatgc
    70501 tcggtcaact tctgccataa tgggtcgatc caaatggcag accttttctt tcaatcttga
    70561 tttatcgatc gttctgatct gctccaacag gataacggag tctctttcta ttcctgttcc
    70621 agtagctttg atcccaatgt gagtcggaag cttaggttta gccatttttg ccgttatggc
    70681 cgcaacaatg atcgtcgggc taaaatggtt acctagatta ttttgaataa ccaaaactgg
    70741 gcgtgttccg ccttgctccg atcccacgac tggagaaaga tcagcaaaat aaatatctcc
    70801 tctttttacc ataggatcct ccttattctt gatattctct tggtatccgc tgtcctaatc
    70861 cgcaagccac ttcatagtgg atcgtaccta attgatctgc tatatcctgc atagtgattt
    70921 ctttgtctgc atttttgccg ataagcgtca cttttgttcc gacaggaaat tcttgaggca
    70981 atcggatcat cagttgatcc atacatacac gcccaatcgt ttcacagtag ttcccttcta
    71041 ccaatagaga aaaaccctgc atctttcgtg gccagccatc tgcataaccg atcggtatcg
    71101 ttccgatcca ctctgcttca ggagtgatat atgtttctcc atagccgatt ccttcacctg
    71161 ctgggagttt tttgacttga atcaattcag aaaccagctc aagtgctggc tgcaatggat
    71221 aaacttccgg tagtgcgtgc ccagagggat taagtccgta cattgctact ccataacgaa
    71281 tcatattgcc gattgtttca tcatgccata aagccgttgc actattgctg acatgtacat
    71341 atcgaggaag ttcgggaagt tttttcagca cttctataaa gcgttctttt tgcagattcc
    71401 aatatgtatc atctgcctga tctgctgtag aaaaatgggt aaataaacct tcccattcta
    71461 atacacgact ttctttgatg aatgcagctg cctcttttac ttcttcaggt gtacaaaatc
    71521 cgatacgacc catcccagtg tctgctttta gatgaatcga aagaggcgtc tctagcttac
    71581 ttttctctaa aacagcagct gctgcttgca accattcccg tgtacctgca gttactgata
    71641 aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac catattcaag ataaggatgg
    71701 gctgagtaaa tccagcttct ctcaactcga ctccttcatc taaatttgaa acacaaaagc
    71761 ccgaagctcc acctgcaact gctgcctcag ctgtttcaat agctccatga ccgtatccgt
    71821 tcgcttttac tactgcaaac aattcttttc cttgcggaag tcttgcaact tcatttgcta
    71881 cattttcagt tatagctttt ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa
    71941 ccatcgtttt tccttctttc tagtcttcca aaataatttg tgcaaaagct gtttcatttg
    72001 tatgagtaat actgacccat acattgcctt catgaggtga acgcgtcaca acaggctgac
    72061 cattttcatt ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc ccagttcccc
    72121 aagctttaga gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct acttggcgat
    72181 gaaaaggcaa ggactggaat agtttcattt catcagaggt caagattcgt gcaatgaatt
    72241 ttggtttttc ttcgatcagc cgagtgatcc taggaagttc gactgcatcg atcccaattc
    72301 cttttatcat atgcttttct cttttcaata aatttagaac actctcattc taccaaatta
    72361 taaccatttt gccacaaata agtccacaaa taacaaaaat gaaagtttct cttaaaaaag
    72421 agaaactttc attttatgtt taatcgttat ttgaacggat tacaaatccg cgttttttat
    72481 cattgtgacg tttaccgcca tctttttggc tgttattatt tttgctataa ccgccttttg
    72541 atttgtttcc acgataatta ccgccgtcac gacgattacg gttgtttccg ccaccacgtt
    72601 tgccattttt attgtatcct tttttctgca ttggcaatgg gcgttctggt gtgatcttca
    72661 ctggcactgc atcagctgga tcttttgctg tcgtttttag caaaagtgca actaaatctt
    72721 gtgcagaata ttcttctaat aatttatctg ctgtttgcag gtatttatct agaccatttt
    72781 ctgcaagttt tgtttcgatt tgttcaactg cagcacctaa ttgacctttg aatgcttctt
    72841 tttcggttgg tggacgtaaa gtcgtcatcc gttttttcgt caagttttca atcacatgca
    72901 agtagtccat ttcatttggt gtaacgaaag tcacagacat accgcctttt ccggcacggc
    72961 ctgtacgtcc gatacggtga acgtagcttt ctggatcttg agggatatca tagttgtaca
    73021 catgagtgac acctgaaata tctaatccac gtgcagcaac gtctgtcgct acaaggatat
    73081 ctaaatgacc gctcttaaat gaacgcaata cgctcattcg tttttgttga gacaaatcgc
    73141 catggattcc ttctgcacgg taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac
    73201 ggcgttttgt acgtccgaag acgattgtta attcaggtgt ttgaacatca aacaagcgag
    73261 tcatgatatc gaatttttcg tattcttttg cgcggacata gtattgatcg atcaagtcag
    73321 ctgtcatttc tttcgctttg atcttcacat gatgtggatt tttcatgaat ttaacgccaa
    73381 tattcttgat agctggcggc atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa
    73441 cttgtgaaat gattttttcg atatcttcca agaagcccat attcaacatt tcatctgctt
    73501 catccaaaac taatgtttga acggttccaa gtttcaatgt gtggcggtta atgtggtcaa
    73561 gcatacgtcc tggtgtaccc acaacgatat gtggacgatc tttcaagcca cggatctgac
    73621 ggccaatatc tgctccgccg taaacggctt gtacacgtac ttttttgtcg cgccctaaac
    73681 gatataattc ttcttgtgtt tggatagcta attcacgtgt tggtgcgata actaaacctt
    73741 gtaattcgtg acgatctgga tcgatttttt ctaacattgg taatccaaat gcagctgttt
    73801 tccctgtacc tgtctgtgct tgtccaataa cgtcttttcc agctaatgct agtggaatcg
    73861 tttctgcttg gataggcgtt gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt
    73921 ctggtgataa ttctagttct ttaaatttca aatgaatcct cctatatggt tgttaactga
    73981 ggatgtagct tttttgctac cccgtcgatt tgttcggtca aagtgtattg tttctttttg
    74041 acagtggcca cgcacgtttt tatccattct tagcccctgt ggcaccaccg aaaaatcact
    74101 ttgcgaagtt taggcattag aaaagccaaa cgggtcgcgt tcagctttat cattattatg
    74161 acatctggct tcaacaagtt gtttctatgg caaaaccagt ccaatttctc ttcatctttc
    74221 ggcaataggt tattctaaca tagtttttcg ttttctgcaa gtttacagat ttttatttca
    74281 tttgctgaag ttgttctacc acttcaaata agcccatgct gttgctgcct tttagcataa
    74341 tactatctgt tgcttcaaga ttttgtttga tagcatcaat caatttattc ttttcttttt
    74401 caaaatagaa cacaggtagt tgggggtatt tttggttcaa ttcttctttt agtgcatgca
    74461 tttgttcccc ataaaggaaa atcatggcga atttctcagg atcgatatgt tcactcattt
    74521 gcgcatgcat atttaaagac tctttcccta attccagcat atctgctaac acagcgattc
    74581 tgcgtccttt tgttggcaga tttttaaaag tatctaatac cagtcccatg gctgttggat
    74641 ttgcattgta gacatcgctc aaaatagctg caccatttcc tgccgttagc cattcggttc
    74701 tgttttgagt gatagaaaca tgggcgagcc cttggcgtat ctcaggtatg gacagtccaa
    74761 agaagcgccc gatcgtatac gcaatcaaag cattcgttac attataactt ccaggcagcg
    74821 ggatcgtaaa tgtttctcct tctactacaa aatcggtttg tgcttttcct tttgctgtca
    74881 cttccgcatg aacatcgcca tttccaatac cgaatgttgt gactgtttgt actaatgttg
    74941 ccgttaaagg tgtcagcaac ggttcatctg cgggcacaac gagtaatccg tctgatacaa
    75001 gtccgtcagt gatttccatt ttggctttag cgattccttc tcgcgaccca agattttcta
    75061 tgtgtgcttc accgatcatt gtgattgctg cagcttttgg ttgagccata agtgaaagtt
    75121 ccgtgatttc attggcgtga tccatgccca tttccaaaac gatcttttct gtatcttggg
    75181 gcatgtgcaa aatcgtgtaa ggaagaccaa tattattgtt gtaatttcct tgcgtcttat
    75241 atgttttgta tttttcagac aagacagacg cggttagatc tttcgtggtg gttttaccat
    75301 tgcttcctgt aattgcaacg acttctgcgc ctactttttc caagtaatag gttgccaatt
    75361 gctgcatggc tttcaaagga tctgctacag gaataactgg aaaatttttt ggagcttgtt
    75421 caacaggcaa gctccaaaat gaagcagatg ctccgtttgt gatcgcttgc tggataaatt
    75481 catgtccatc tcgcgtacca gcaagaggaa cgaataaact tccttcggtt atcttccggc
    75541 tgtcaaattc gacattggta atctttgtca gtgcagagca ttcgctgccc acggcttgtg
    75601 caatttcttg tattgttaga ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg
    75661 tttggcctaa acggcgggtt cgcatgactc tcacttccaa gtaaatttct ctattcgtat
    75721 ttagaagcta gaatgaacgc ggttacgcaa tcactccagc ctcttcttta tttctcttta
    75781 ttcatttttt tcaaaaaaag attgacgctg atggtatcga ttcattccta actggatcag
    75841 ttcttcaatc aaatcaccgt atttcaagcc catattttcc cataagagtg ggtacatact
    75901 gaactccgta aatcctggca tagagtttaa ttcattcagg aataattcat ttttatttgt
    75961 caaaaagaaa tcgcaccggc tcaatccgct tccacctaac atcgtgtaag ctaacttcgc
    76021 gtactcttgc gctttttgat aaacttcttc tggcacttcg gctggaatct gcatttcgat
    76081 tttattattg atatattttg cttcataatc atagaatgct acgtctttta cgacttcacc
    76141 aggcaaagtc gtccgaacgt cttcatttcc taatacagca acttcgattt cgcgcgcttc
    76201 aattccttgt tcaacgattg ctcgagaatc atactgatag gctgttgcta aagcattttg
    76261 cagctcttct cggttttctg cttttgtaat gccgacacta gaacccatat tcgccggttt
    76321 gacaaacatc ggataaagca aagaaccttc acattgatca aatacttttt taggattttc
    76381 tttccattga ttcttaagta ctggtacata aggaacttgc ggcacaccag cagcttgtaa
    76441 aatatacttg gtcatgattt tatccatggc acatgcactg gtcaatacgc ctgcgccgac
    76501 ataaggcata ttcaatgtct ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc
    76561 atgtaaaact ggaaaaacga tggctccttc ttctttgatt tcgcccggat tgatcacttt
    76621 tcctgtaaag ccttcctctg tctgtccact tgggtcccat gaaagatgca agacatcttt
    76681 gctggcaggt ttttctgtta gtaatggacc tttgacccat tgtccttctt ttgtaataaa
    76741 tacgagttga acttggtaat aattataata aatggcattt aaaactgaaa atgcggaaag
    76801 aatggacact tcatgctctg cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt
    76861 gtcctcctgt attctaaatc gttcagtgat ttactctcat attttagcac aaaacaaaaa
    76921 taaacgaata acttatttat aaaaaaacga gccagcttct aaaagaacga gcgtcattta
    76981 ttttttgtca gctagattat ttgtcccgtt ttttataaaa agttatgata aagaaaagga
    77041 gggatgatga tggcgcaaac aaatccacgc agccgcttgg cagaaatcat tcgagtgttc
    77101 atcaattaca atgtagtgcc aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa
    77161 gcctttgaag aactaggccc caccttcatc aagataggac agatgctttc tgttcgagaa
    77221 gatttgcttt cttctgcctt cactcaaaca ttcaaaacat tacaagatag tgtacctagc
    77281 gacacatttt caacggtcaa aaagacgatc gaaacagaac tttctctttc attatcagat
    77341 atattcgatg atttttcaaa atcgcctttt gcctctgctt ctatggggca ggctcatcgt
    77401 gccaagctga agaacggtga cgcggtagtc gttaagatcc aacaccctaa catcgccgaa
    77461 gagatccgac ttgatcttca actatttgaa cgagcgatcc ctttgatcaa atacattcct
    77521 gaaactagcg tagttgatct aaaaggggta ctccaagaag taaaacgttc cttgatcaat
    77581 gaaatggact tcttaaaaga aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc
    77641 tggaaagaga tccgcagccc caagatttat gacgcttttt gttcgaaaaa agtgatcgtg
    77701 atggaagaaa tgtctggtaa aaacttgaat catttaatga acgccgaaaa caaaactgaa
    77761 acttttatca ctggaattca aaacaagcag ctgaaacaag aagttgcaaa gcttttagtt
    77821 gagaacttta tgaaacaggt gtttgatgat ggttttttcc atgctgatcc tcatccaggg
    77881 aatctgctat tccatgtact aacaaaagaa gaacagacac aagctagccg aaaaactgaa
    77941 acagtccatg aaaaagagtt cggttccttt gcttttcgag cctccacttc tgcagaagac
    78001 cctgttgcgc catataccat caactacatc gattttggta tgatggggca tctttctgca
    78061 ggacttaggc aaaaactcac acaagccgtt ttagcgctgt atacgaaaga tgcttatcga
    78121 atcgaaaaag cggtccttcg actttgtcag caagaaggct cgtttgatga aagtcggttc
    78181 caccaagaac tgacaagttt tcttgaacag tattatgatt ctcctattga tgaaatcaac
    78241 ttgcaagaag tattcacaca tgtcgtgacg atttgtcatc aaaataatct gcagttcgat
    78301 cgtgatatca cgcttttgct gaaagcattt gggacattag aaggggtgat ccgcgtcctc
    78361 gatcctgaag tttccctcat ggaagtcgct tcgccttttg ctcaacatta ttttctcact
    78421 catctggatg tagaagatac attgaaacaa agcggattag acttattgga agggatgaag
    78481 gctgcgccta agatccctca acagctccac catctattag aaatgtggac ttcgggacaa
    78541 ggaaaagtca atttggaatt gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg
    78601 atcaatcggc ttgttttcgg tatgatttta gctgcattga tcgtagggtc ctccctgctt
    78661 gtccaagcag cacctgtaga aaacgcagag gtcgtttcct tattagggat atttacgtat
    78721 gctattgccg cattcgtcat tatttttttg gcaatcgatg cattgatcca aatgtataag
    78781 aagaggaaaa agtagaaaaa ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg
    78841 gttttacata aattgtttct caaatttttg tgaaaattcg gcttattttt cgaagaggct
    78901 acttctgaaa caccgagaat aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa
    78961 caatcaaaaa gggaacatgg aacacattta tcctgtttcc ctgttccctt tttgatgtat
    79021 tcgagcataa ataaaatacc ttcgttacct actatcttct gtacttggta aaccttagtc
    79081 aattgccttt tttaacgctt gacgaaaaga catatgatct ttctccgtaa agggttttgg
    79141 accctttgtc cttatgccag cttttcttaa ctttccacta tgataacgtt gttctagaag
    79201 cgtgccgata ttcgcttcgt caaattcttt tccagagtga tggaacactc gcacaccctt
    79261 cggcaataca agtgaagcta aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac
    79321 cataccgacg attttataat ccgcactatc agctccgcgg tctacgtaga cgatttggac
    79381 atgattagcg tattctttgg tcgtataatg gtccacactg gtcacgataa ccacgtcaag
    79441 accataggat tctcctaatt ttatgacatc ttcttttact ggagaaccat ctccatcaat
    79501 gaatagcctc attctttttc gccttcttcc ataaatgata aacagttact aatccgctca
    79561 gtaaaagacc aacattgatc cattttgcca agataagcag ttgtacactt tctatcggca
    79621 ctgattcatg gttcagcgtg atcaataata gatggattgg ctctcccatt tcttttgcta
    79681 aaacaaacaa aagcgtcatc aataaaaact ggtggatcac actcccttta ttttgagtag
    79741 tatacttgac aacgatccgg cgatccattg gaatagctgt atcagtttga taatattcct
    79801 tatttagcca gctgcctgtt actacgtgcc agaaactaag cagaaagaca cctatcgcaa
    79861 aaccatttgg aatcaagagg accaataaag aagaactcag tccaagcaat tgcatagcca
    79921 atggagacca tgttaaacgt ttcaaaatcc atggtccgac tatttttgct gcaatcaagc
    79981 ctgctgcata aggcaaatac ataaaaatcg aaagtttttc taatccgtac agtatgccaa
    80041 cataaaaact gctaaataaa aaaaggaaat tccctagcat tccatttaga aaagtaaaca
    80101 gatttagcca gcttggcaac ttccattcct tcttctggtg tcctaaaaca aacacgaaca
    80161 agatgaacag cagcaagaaa ccataaatag caagatcaaa aagcctttca tcgaataaca
    80221 atcgggcatt tctcaaaaga aacaataagc caaagaatat caaaaataaa acaagctctc
    80281 gcgtagctac cagatttctt ttgatgtctt tgaaatccag ttcgtaacgc ggataatgac
    80341 taaccgtatg gtaggccatg acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct
    80401 gagtcggtgc aggcaactca atcgcttgat acaggagaaa tagccagata gctgcaaata
    80461 agtacttttt cccatccata ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg
    80521 tattagcagg tacaagccat gctgcactca gccctagcaa aacacttgaa aaataaaata
    80581 aaggaaaata aaatacgcct aatattccaa gcaatgaacc agcaccacca atcagcagag
    80641 cgatcatcag caacgtgtaa ctatctagac caaatcgtag actgttgact aaaaatagtc
    80701 cagtcatccg aaaggcataa aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt
    80761 ctgttagtcc caaaaataaa agataaggaa taaaaataac cgtgttactt aagataaagg
    80821 gcgttgtttg tgtaaaatta cgcccaaatc gtttgagttt ttcgaacatg acaatggctc
    80881 cttaaacagt cttataacta atctgctctt cgattttagc attaaagggg aaataaacca
    80941 attttacaga atatattctt gtaaaaaaaa atacagaaag aagcataagc ttctttctgc
    81001 atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag tcttgcccga
    81061 cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc tccaaaatca
    81121 attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta ctttaacaca
    81181 gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga aatccttgac
    81241 cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa acctcgattg
    81301 cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa
    81361 gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact ttatttagca
    81421 aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg taaccgacag
    81481 tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct ttttgaatct
    81541 gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca atgatcggca
    81601 caaaaacttc cggccatact tcttgtatga ctgccatcag ctcatccagt tcattttctg
    81661 ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga ccttgcgctt
    81721 gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt ttcaagaaaa
    81781 tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact agacagatca
    81841 agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta atatctttcg
    81901 tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa ataagctgga
    81961 caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca atcactaggc
    82021 caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc gctactgagg
    82081 agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga acaaatatca
    82141 tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa
    82201 tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca ataatgggca
    82261 tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt ccccacatct
    82321 tttttcattt attaaaactg cctcct
//
LOCUS       DASOUD010000002        77815 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.002, whole genome shotgun sequence.
ACCESSION   DASOUD010000002 DASOUD010000000
VERSION     DASOUD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.002"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            72..1136
                     /locus_tag="U4A29_000079"
     CDS             72..1136
                     /locus_tag="U4A29_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO0679235.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1382..1807
                     /locus_tag="U4A29_000080"
     CDS             1382..1807
                     /locus_tag="U4A29_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO0679236.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1800..2249
                     /gene="nusB"
                     /locus_tag="U4A29_000081"
     CDS             1800..2249
                     /gene="nusB"
                     /locus_tag="U4A29_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO0679237.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2346..3200
                     /locus_tag="U4A29_000082"
     CDS             2346..3200
                     /locus_tag="U4A29_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO0679238.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3201..4538
                     /locus_tag="U4A29_000083"
     CDS             3201..4538
                     /locus_tag="U4A29_000083"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO0679239.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4543..4767
                     /locus_tag="U4A29_000084"
     CDS             4543..4767
                     /locus_tag="U4A29_000084"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO0679240.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4769..5659
                     /locus_tag="U4A29_000085"
     CDS             4769..5659
                     /locus_tag="U4A29_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO0679241.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5668..6486
                     /locus_tag="U4A29_000086"
     CDS             5668..6486
                     /locus_tag="U4A29_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEO0679242.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6593..7042
                     /locus_tag="U4A29_000087"
     CDS             6593..7042
                     /locus_tag="U4A29_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO0679243.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7060..8742
                     /gene="recN"
                     /locus_tag="U4A29_000088"
     CDS             7060..8742
                     /gene="recN"
                     /locus_tag="U4A29_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO0679244.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8797..8913)
                     /locus_tag="U4A29_000089"
     CDS             complement(8797..8913)
                     /locus_tag="U4A29_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO0679245.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(8986..9942)
                     /locus_tag="U4A29_000090"
     CDS             complement(8986..9942)
                     /locus_tag="U4A29_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO0679246.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10113..10481
                     /locus_tag="U4A29_000091"
     CDS             10113..10481
                     /locus_tag="U4A29_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEO0679247.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10651..11082
                     /gene="mraZ"
                     /locus_tag="U4A29_000092"
     CDS             10651..11082
                     /gene="mraZ"
                     /locus_tag="U4A29_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEO0679248.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11103..12062
                     /gene="rsmH"
                     /locus_tag="U4A29_000093"
     CDS             11103..12062
                     /gene="rsmH"
                     /locus_tag="U4A29_000093"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO0679249.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12067..12474
                     /gene="ftsL"
                     /locus_tag="U4A29_000094"
     CDS             12067..12474
                     /gene="ftsL"
                     /locus_tag="U4A29_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO0679250.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRIRKVK"
     gene            12475..14667
                     /locus_tag="U4A29_000095"
     CDS             12475..14667
                     /locus_tag="U4A29_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO0679251.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14693..15655
                     /locus_tag="U4A29_000096"
     CDS             14693..15655
                     /locus_tag="U4A29_000096"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO0679252.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15669..17030
                     /locus_tag="U4A29_000097"
     CDS             15669..17030
                     /locus_tag="U4A29_000097"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO0679253.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17056..18144
                     /gene="murG"
                     /locus_tag="U4A29_000098"
     CDS             17056..18144
                     /gene="murG"
                     /locus_tag="U4A29_000098"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEO0679254.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18159..19379
                     /locus_tag="U4A29_000099"
     CDS             18159..19379
                     /locus_tag="U4A29_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEO0679255.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19535..20860
                     /gene="ftsA"
                     /locus_tag="U4A29_000100"
     CDS             19535..20860
                     /gene="ftsA"
                     /locus_tag="U4A29_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO0679256.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20883..22124
                     /gene="ftsZ"
                     /locus_tag="U4A29_000101"
     CDS             20883..22124
                     /gene="ftsZ"
                     /locus_tag="U4A29_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO0679257.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22141..22818
                     /locus_tag="U4A29_000102"
     CDS             22141..22818
                     /locus_tag="U4A29_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO0679258.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22832..23431
                     /locus_tag="U4A29_000103"
     CDS             22832..23431
                     /locus_tag="U4A29_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO0679259.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23446..23733
                     /locus_tag="U4A29_000104"
     CDS             23446..23733
                     /locus_tag="U4A29_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO0679260.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23782..24564
                     /locus_tag="U4A29_000105"
     CDS             23782..24564
                     /locus_tag="U4A29_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO0679261.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24585..25292
                     /locus_tag="U4A29_000106"
     CDS             24585..25292
                     /locus_tag="U4A29_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO0679262.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25554..28352
                     /gene="ileS"
                     /locus_tag="U4A29_000107"
     CDS             25554..28352
                     /gene="ileS"
                     /locus_tag="U4A29_000107"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO0679263.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28401..29927)
                     /locus_tag="U4A29_000108"
     CDS             complement(28401..29927)
                     /locus_tag="U4A29_000108"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEO0679264.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29942..30589)
                     /locus_tag="U4A29_000109"
     CDS             complement(29942..30589)
                     /locus_tag="U4A29_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO0679265.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30773..31102)
                     /locus_tag="U4A29_000110"
     CDS             complement(30773..31102)
                     /locus_tag="U4A29_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEO0679266.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31279..32007
                     /gene="sfsA"
                     /locus_tag="U4A29_000111"
     CDS             31279..32007
                     /gene="sfsA"
                     /locus_tag="U4A29_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEO0679267.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32023..33036
                     /locus_tag="U4A29_000112"
     CDS             32023..33036
                     /locus_tag="U4A29_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO0679268.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33036..34313
                     /locus_tag="U4A29_000113"
     CDS             33036..34313
                     /locus_tag="U4A29_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO0679269.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34376..37078
                     /locus_tag="U4A29_000114"
     CDS             34376..37078
                     /locus_tag="U4A29_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEO0679270.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37230..37547
                     /locus_tag="U4A29_000115"
     CDS             37230..37547
                     /locus_tag="U4A29_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0679271.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37577..37897
                     /locus_tag="U4A29_000116"
     CDS             37577..37897
                     /locus_tag="U4A29_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO0679272.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38005..39465
                     /locus_tag="U4A29_000117"
     CDS             38005..39465
                     /locus_tag="U4A29_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0679273.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39533..39754
                     /locus_tag="U4A29_000118"
     CDS             39533..39754
                     /locus_tag="U4A29_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679274.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39785..39967
                     /locus_tag="U4A29_000119"
     CDS             39785..39967
                     /locus_tag="U4A29_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEO0679275.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39967..40380
                     /locus_tag="U4A29_000120"
     CDS             39967..40380
                     /locus_tag="U4A29_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEO0679276.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40503..41684
                     /locus_tag="U4A29_000121"
     CDS             40503..41684
                     /locus_tag="U4A29_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0679277.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(41755..41919)
                     /locus_tag="U4A29_000122"
     CDS             complement(41755..41919)
                     /locus_tag="U4A29_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679278.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            42073..42146
                     /locus_tag="U4A29_000123"
     tRNA            42073..42146
                     /locus_tag="U4A29_000123"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42107..42109,aa:Arg,seq:cct)
     gene            complement(42215..43354)
                     /locus_tag="U4A29_000124"
     CDS             complement(42215..43354)
                     /locus_tag="U4A29_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO0679279.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(43653..44288)
                     /locus_tag="U4A29_000125"
     CDS             complement(43653..44288)
                     /locus_tag="U4A29_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679280.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(44401..45036)
                     /locus_tag="U4A29_000126"
     CDS             complement(44401..45036)
                     /locus_tag="U4A29_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEO0679281.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(45070..45531)
                     /locus_tag="U4A29_000127"
     CDS             complement(45070..45531)
                     /locus_tag="U4A29_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEO0679282.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(45661..46092)
                     /locus_tag="U4A29_000128"
     CDS             complement(45661..46092)
                     /locus_tag="U4A29_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO0679283.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(46110..46430)
                     /locus_tag="U4A29_000129"
     CDS             complement(46110..46430)
                     /locus_tag="U4A29_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0679284.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            46729..47505
                     /locus_tag="U4A29_000130"
     CDS             46729..47505
                     /locus_tag="U4A29_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEO0679285.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            47520..47723
                     /locus_tag="U4A29_000131"
     CDS             47520..47723
                     /locus_tag="U4A29_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0679286.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            47739..48077
                     /locus_tag="U4A29_000132"
     CDS             47739..48077
                     /locus_tag="U4A29_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEO0679287.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            48064..48243
                     /locus_tag="U4A29_000133"
     CDS             48064..48243
                     /locus_tag="U4A29_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679288.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(48286..48756)
                     /locus_tag="U4A29_000134"
     CDS             complement(48286..48756)
                     /locus_tag="U4A29_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679289.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            48843..49541
                     /locus_tag="U4A29_000135"
     CDS             48843..49541
                     /locus_tag="U4A29_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEO0679290.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            49719..50060
                     /locus_tag="U4A29_000136"
     CDS             49719..50060
                     /locus_tag="U4A29_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679291.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            50053..50724
                     /locus_tag="U4A29_000137"
     CDS             50053..50724
                     /locus_tag="U4A29_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEO0679292.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            50730..51416
                     /locus_tag="U4A29_000138"
     CDS             50730..51416
                     /locus_tag="U4A29_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679293.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            51419..52168
                     /locus_tag="U4A29_000139"
     CDS             51419..52168
                     /locus_tag="U4A29_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0679294.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            52180..52449
                     /locus_tag="U4A29_000140"
     CDS             52180..52449
                     /locus_tag="U4A29_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679295.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            52454..52618
                     /locus_tag="U4A29_000141"
     CDS             52454..52618
                     /locus_tag="U4A29_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679296.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            52611..52913
                     /locus_tag="U4A29_000142"
     CDS             52611..52913
                     /locus_tag="U4A29_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679297.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            52910..53071
                     /locus_tag="U4A29_000143"
     CDS             52910..53071
                     /locus_tag="U4A29_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEO0679298.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            53068..53373
                     /locus_tag="U4A29_000144"
     CDS             53068..53373
                     /locus_tag="U4A29_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679299.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            53373..53729
                     /locus_tag="U4A29_000145"
     CDS             53373..53729
                     /locus_tag="U4A29_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEO0679300.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            53716..53934
                     /locus_tag="U4A29_000146"
     CDS             53716..53934
                     /locus_tag="U4A29_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679301.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            53931..54350
                     /locus_tag="U4A29_000147"
     CDS             53931..54350
                     /locus_tag="U4A29_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEO0679302.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            54347..54904
                     /locus_tag="U4A29_000148"
     CDS             54347..54904
                     /locus_tag="U4A29_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEO0679303.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            54901..55197
                     /locus_tag="U4A29_000149"
     CDS             54901..55197
                     /locus_tag="U4A29_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679304.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            55274..55687
                     /locus_tag="U4A29_000150"
     CDS             55274..55687
                     /locus_tag="U4A29_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEO0679305.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            55996..56148
                     /locus_tag="U4A29_000151"
     CDS             55996..56148
                     /locus_tag="U4A29_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679306.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(56145..56420)
                     /locus_tag="U4A29_000152"
     CDS             complement(56145..56420)
                     /locus_tag="U4A29_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679307.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            56874..57080
                     /locus_tag="U4A29_000153"
     CDS             56874..57080
                     /locus_tag="U4A29_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679308.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            57276..57443
                     /locus_tag="U4A29_000154"
     CDS             57276..57443
                     /locus_tag="U4A29_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO0679309.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            57469..57813
                     /locus_tag="U4A29_000155"
     CDS             57469..57813
                     /locus_tag="U4A29_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO0679310.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            57818..58099
                     /locus_tag="U4A29_000156"
     CDS             57818..58099
                     /locus_tag="U4A29_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679311.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            58202..58516
                     /locus_tag="U4A29_000157"
     CDS             58202..58516
                     /locus_tag="U4A29_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEO0679312.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            58494..60188
                     /locus_tag="U4A29_000158"
     CDS             58494..60188
                     /locus_tag="U4A29_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO0679313.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            60208..61386
                     /locus_tag="U4A29_000159"
     CDS             60208..61386
                     /locus_tag="U4A29_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO0679314.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            61349..62035
                     /locus_tag="U4A29_000160"
     CDS             61349..62035
                     /locus_tag="U4A29_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEO0679315.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            62035..63195
                     /locus_tag="U4A29_000161"
     CDS             62035..63195
                     /locus_tag="U4A29_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO0679316.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            63205..64080
                     /locus_tag="U4A29_000162"
     CDS             63205..64080
                     /locus_tag="U4A29_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679317.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            64077..64388
                     /locus_tag="U4A29_000163"
     CDS             64077..64388
                     /locus_tag="U4A29_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679318.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            64378..64731
                     /locus_tag="U4A29_000164"
     CDS             64378..64731
                     /locus_tag="U4A29_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEO0679319.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            64721..65122
                     /locus_tag="U4A29_000165"
     CDS             64721..65122
                     /locus_tag="U4A29_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679320.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            65115..65519
                     /locus_tag="U4A29_000166"
     CDS             65115..65519
                     /locus_tag="U4A29_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679321.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            65531..66139
                     /locus_tag="U4A29_000167"
     CDS             65531..66139
                     /locus_tag="U4A29_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO0679322.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            66159..66521
                     /locus_tag="U4A29_000168"
     CDS             66159..66521
                     /locus_tag="U4A29_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679323.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            66723..70154
                     /locus_tag="U4A29_000169"
     CDS             66723..70154
                     /locus_tag="U4A29_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO0679324.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            70205..70942
                     /locus_tag="U4A29_000170"
     CDS             70205..70942
                     /locus_tag="U4A29_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO0679325.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            70952..73243
                     /locus_tag="U4A29_000171"
     CDS             70952..73243
                     /locus_tag="U4A29_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO0679326.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            73267..75393
                     /locus_tag="U4A29_000172"
     CDS             73267..75393
                     /locus_tag="U4A29_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679327.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            75410..75559
                     /locus_tag="U4A29_000173"
     CDS             75410..75559
                     /locus_tag="U4A29_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679328.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            75556..76002
                     /locus_tag="U4A29_000174"
     CDS             75556..76002
                     /locus_tag="U4A29_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679329.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            76004..76141
                     /locus_tag="U4A29_000175"
     CDS             76004..76141
                     /locus_tag="U4A29_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEO0679330.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            76179..76472
                     /locus_tag="U4A29_000176"
     CDS             76179..76472
                     /locus_tag="U4A29_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679331.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            76469..76693
                     /locus_tag="U4A29_000177"
     CDS             76469..76693
                     /locus_tag="U4A29_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO0679332.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            76690..77715
                     /locus_tag="U4A29_000178"
     CDS             76690..77715
                     /locus_tag="U4A29_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO0679333.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        26586 a        12643 c        16281 g        22305 t
ORIGIN      
        1 tttaactgtc gtgtacgaag aaaagtttgc tataatggaa aaaagcgtgc aaagaaagag
       61 ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa aaaaatgcaa gaagaaaatt
      121 tggattcatt tttggttacc agtccgtaca atcttcgtta cttaacgaac tttacaggta
      181 cgacaggatt agctgttatt actttggaaa aagcattttt tattactgat tttcgttata
      241 ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat taaaaatgtc ggacctattt
      301 ttgatgaggt agcaaactta gtacgcaaag aagaactgca ctcattagct tttgaggaaa
      361 caacagtctc atttttagaa tattcggttt tagaagaaat catcgatgcg gaattagtac
      421 ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga tgaagaagaa atcgctatta
      481 tcgaaaaagc atgtcatatt gcggatatgg catatgatca catcctgaaa atgatccaac
      541 cgggcatgac tgagattgaa gtcgcaaatc aactagactt ttatatgcgt tctcttggcg
      601 cttcaggtgt ttcttttgat acgatcgttg ctagcggatt gcgttcggct atgccgcatg
      661 gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat cacattggac tttggttgct
      721 attacgaagg gtacgtttcc gatatgacac gtacattcgc tattggtgat cctggggaaa
      781 aattaaaaga aatttatcaa atagtcttag atgctcaatt agcagttatt gatgctgcaa
      841 aaccaggaat gactggtgtc caattagatg cagttgctag agattatatt tcaaaacatg
      901 gttacgggga agcatttgga cattctacag gacatgggat cggtttagag atccacgaag
      961 gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc aggaaatgtg atcactgatg
     1021 aaccagggat ctacttacca ggaatcggtg gagtacgtat tgaggatgac ttgttgatca
     1081 cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga attgatcatc ttatagtttc
     1141 atcaatagaa gaagtaagcg taaaaatcag tgttcacaga ctttgttata gaaaatcact
     1201 cgatttaatg atacactata gaagaacttt taagctgtaa gagataataa agttgggtgt
     1261 tttctccaat tcatagcagt ttgcagctga tcggcggcag ttttcttacc acgtgatcac
     1321 tatgccgcat catagagaat tcggatgtat ctcgtgctta ggcaaaggga ggaataaata
     1381 aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa gatttaggag agatcgtgat
     1441 cgctcctgaa gtcatcgaag taattatcgg gattgcagct tctaaagtag aaggcgtata
     1501 cggaatgcgc ggaacttttg ccagcaatgt cactgaatta ttaggacgtg cagctcacgg
     1561 aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa gtagatcttt actgttatct
     1621 agaatatggt gtttcagttc ctaaagtagc aatggaaatg caggaacggg tcagccagca
     1681 agtccttttc atgacggata tcgatttatc tgaagtcaat atccatgtag tagctgttgt
     1741 tcctgaaaaa ttagaacaac caacaatcga agaacttttt gaagacgaag aggaagagaa
     1801 tgaataaaga attatcaaga cacgagattc gggaaatggc attgcaggct ttatttccat
     1861 tagacttcaa tgcagaccta acaaaagaag atgcgatttt caacgcaatc gaattagacc
     1921 accgcgacat gatcaacgaa gatgaatcag agtttgttcc tgtttatctt gatacgctag
     1981 ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt gattgaaaaa catttaaaaa
     2041 acaattggca catcaatcgg atctcaaaaa tggatttggt gatcttgcgt atcgcgattt
     2101 ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc tttaaatgaa gcaatcgagt
     2161 tggcaaaaac atttagtgat gaccgttcac gtaaattcgt caacggtgtt ttatccaatg
     2221 tattgaaaga attagaagct ggggcgtaag tcctagcttt tttctatgaa tcatcataaa
     2281 cctgttataa atcgggaaaa ttctatggta gaatgaaaac acaaaaaata tctgggagtg
     2341 gttagatggc agaattgatt aacggaaaag aattagctga aaaaatgcag gcagagattg
     2401 ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc cggattagtc gttttattgg
     2461 taggggaaaa tccagccagc caaatctatg taagaaataa agaacgtgta gcaaaagaaa
     2521 tcggaatcca ttcattggta gagcgttatc ctgagacgat ttctgaagac gaactacttg
     2581 cagaaattga aaaatacaat caagatccta gttttcatgg tatattggtc caattaccat
     2641 tgccaaagca tatagatgaa gaaaagatcc tgttagctat ccgccctgaa aaagacgtag
     2701 acggttttca tccgatgaat ttaggcagac tccttgcggg taaaccggac aagattcctt
     2761 gtactccata cggcatcatg aaaatgtttg aagcttattc tatcgatccg gcgggtaaac
     2821 gagcagtagt tatcggacga agcaatatcg tcggtaaacc gatggctcag ctactcttga
     2881 tggcagatgc aactgttact gttgctcatt ctaaaaccga aaatctggct gaactggcac
     2941 aagaagcaga tattttagtc gtagcaatag gacgtggaca ttttgtcaca aaagaattcg
     3001 taaaaccagg tgctgtagtg atagatgtag ggatgaatcg tgatgctaac ggtaagctga
     3061 tcggtgatgt gaaatttgat gaagtggagc ctattgcagg ctggatcact cctgtgccaa
     3121 agggtgtagg gccaatgact atcacgatgc tgatgtatca aaccgtaaaa tctgccgaag
     3181 cttttggagc aggtgaatag atggcacaag aatatttaac tgtcactaca ttaacgaagt
     3241 atttgaaaag aaaatttgat gccgatcctt atttagaacg cgtatattta acaggcgaaa
     3301 tatcgaattt ccgtttacgt gccaatgccc atcaatattt cagcttaaaa gatgaccatg
     3361 caaaaatatc tgctattatg ttcaaaggag cattccaaaa attacgattc cagccaaaag
     3421 aaggaatgaa agtccttgtc gttgggcgta tttcattata tgaagcggga ggttcttatc
     3481 aaatctatgt agagcacatg gagcctgatg gaataggtgc tttatatcaa gaattggaag
     3541 aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg gcctaaaaaa atgctgccac
     3601 gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg agcggttatc cgcgatatca
     3661 tcacgacggt gaaaagaaga tatccaattg tccaattggt tttgttccca acagttgtac
     3721 aaggaaacca agcagcggac aatgttgtca ggaatatcca gagagtagaa gagatgggca
     3781 atttcgatac aatgatcatc ggtcgtgggg gtggttcgat tgaggattta tggccgttta
     3841 atgaagagcg ggttgctcgg gcgatttatc aagctaatac gccggtgatt tcatctgtag
     3901 gacatgaaac agataccacg atcgctgatt tagtagcaga tgttcgggct gccactccaa
     3961 cagcagctgc agaattagct gttcctgttt tgtcagaaga gttgatgcgc attcaagaac
     4021 ggcaagctag attgcagcag gcgtttgtac gccagatcca gcgcaaacaa gaacggttcg
     4081 agcgtgctcg acgttcctat gtatttcggc agcctgaaag attgtatgaa ggtcagacca
     4141 tcaaattaga ccagttgaaa cagcgattat atcaagctac acagcaaatt tatcatgcga
     4201 aagaaaaaca aacgatcgca ttaagccacc aattggaaca agtggctccg ctttatcgag
     4261 ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg cttagaagag aaaatccaac
     4321 aatatatgaa acaacagcag cagcgttttg ctcaagctgt tcaatctctt gatttgttga
     4381 gtccattgaa aatcatggga agaggctaca gctatacgac gttaaatgat caagtagtaa
     4441 aaagtgtcac cgagattcat caaggggatt tgctgacgat ccattatcaa gatggaatcg
     4501 ttaaaggaca agtacaagag atagacaaag gagaataaaa atatggaaaa tccaactttt
     4561 gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt tagaacaagg agacgttcca
     4621 ttagaatcag ctctagattc gtttaaacga ggaatggaat taagcaaaca ttgccaagat
     4681 acattagcta aagcagaaga gaccttgacg aaaatgatga cagaatcaaa tgaagaagtt
     4741 gattttgacg gaaacgagga aagctgagat gagattaact gacttttcag ctattcatct
     4801 acctgctgta gaaaaagaaa tcttgtcttt tttagatgag catacaacgg atagaggact
     4861 ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa agaatacgtc ccttattact
     4921 tttaacggcc gttgcttcct ttgatgagcc tattgatgtt cccgtctatc aagtagcagc
     4981 tgctttagaa atggtgcata cgtattcctt gatccacgat gatttgcctg cgatggacaa
     5041 tgatgatctt cgtagaggaa aacctaccaa tcataaagta ttcggcgaag cattagctat
     5101 attggccgga gatggtttgc ttacaggagc attccagcta atcagtatgg cacacttagg
     5161 taattctcct aagcttctgc tgcttcagca attagctgtt tgtgcgggat cacaaggtat
     5221 ggttgctggg caagctgcag atatcgaggg cgaatcgaaa aagctttctt tagaagaatt
     5281 agcatttatt catgaaagaa agacagggca tttgattcgt tatgccttgc tggctggtgg
     5341 catattagcg aagcaacctg aagaaatctt attgctactt caacgattag ccgaacattt
     5401 aggtttagct tttcagataa gagatgatct actcgatgtg attggtacaa cgaaaacatt
     5461 agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat acttacccgc gtttattagg
     5521 cctagaaaag acacgtgagg cgttggagat tgagctgtta tccgccaaca agatcataga
     5581 caaattagaa gaggacgttt tttcttttga tggcgggctg atgcgccaga tgatcaagca
     5641 atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac gcgtagatgt attagcggtc
     5701 aaacaaggat tatttgaaac aagagaacaa gccaaacgtt cagtgatggc tggtttgatt
     5761 tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa aaattccagt gagtagtgaa
     5821 ttaagagtca aaggtaaaaa acttccttat gtttctcgag ggggattaaa attagaaaaa
     5881 gcactcaaag catttgatct atctgttgaa ggaaaaacat tgttagatat tggtgcatcc
     5941 actggcggat ttactgatgc agctttgcaa aatggcgcaa aaatgagtta tgctttagac
     6001 gttggctaca atcagctagc ttggaaaata agacaagatc ctagagtcgt cgtaatggaa
     6061 agagtgaatt ttcgttatgc aaaacccgaa gactttacct taggaattcc agaagttgct
     6121 gttatcgatg tttcttttat ttcactaaaa ctgatgctgc ctccattaca tgctatttta
     6181 aaagaagagg gagaagtgat tgctttaatc aaaccccaat tcgaagcagg cagagaagct
     6241 gtagggaaaa acggaattgt tcgtgaccct cagacccatc aaaaagtttt agaagacatt
     6301 ttgtcatttg cggcacaagg tggatatgat gtgctggaat taagttattc tccgatcacg
     6361 gggggagaag gaaatatcga gttcttggct cacttgagga aagtacccga aagcggaaca
     6421 atcaattcag ctattaatat ggcagaagtg gtttcaaatg cacacgaaca gtttgaccat
     6481 aaataaattg taactgtgaa aatcaagtga gaagtatgcg gattcttctt tattatacca
     6541 aggaaataag ctatcattga ttaagaagaa tatgctaagg agcgaagacg taatgagaaa
     6601 aaaagatcgg catcgtttga ttactcgatt gttgagtgag caagatatcc gaaagcagga
     6661 agaatttgtt gaaatcctga aaagcaaagg aatatctgtt actcaagcta cgatttccag
     6721 agatataaag gaactgaaac tgatcaaggt accggcttca gatggcggct accgttatag
     6781 cataccagct gaaacaaacg aagatgtaaa tactaaattg gaaaaactat taaaagacgc
     6841 tttcgtggca gttgaccaaa tggaaaaatt tgttattttg aaaacattgc cgggaaatgc
     6901 ttcggcagca gcaaatttga ttgataaacg ttacaagaaa gaattatttt cgattatcaa
     6961 tgatgacgat aatgttttga tgattacacg aactgaagac caggcagttt tattgaagaa
     7021 agacttcctg cattaccttt aagttgagga gcgaatgtga tgctacaaga aatcagtatt
     7081 actaactttg cgattattcc ggaattgcgg ctttcttttc acgaaggaat gaccgcgctg
     7141 accggagaga ctggtgcggg taaatccatt attatcgatg ctttaggtct gttagcagga
     7201 ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa aatgtgtgct tgaagggttg
     7261 tttgaactac caaaacaaga aggattctct gaactgatgg ttgaattagg gatcgaaaca
     7321 gatgaggaca atctgattgt tcgacgggat atgtccttaa ctggaaaaaa tgtttgtcga
     7381 gtgaatggtc atattatcac attagctaat ctgagaagaa tcggcagtta tttagtagat
     7441 atccaaggac aaaacgaaca tcaggaactg ctccagcctg aatcacatct tgctttattg
     7501 gatcgttttg gagatgtagc tttccaacag aagaaaaaga gttatcagca agaatatatc
     7561 tcttatcgtg agctggaaaa aagggtacga aaagtgcagc aaaatgaaaa atcctatgtt
     7621 cagagaatag atatgctcca tttccagcaa gaagaaatag ctgctgctga tttgcaagtc
     7681 ggagaagaag agaaattaaa agaagaaaga gaaaaactta gcaattatca aaaaattgct
     7741 gatggattag ctgcaggtta cggagcactg acgaataatg aacaaaacag tgtagatggt
     7801 gttggcttgg ccgtttcaga actgcaaggg atcgcccatc tagatgtgga gtatgaagca
     7861 atttatgaaa atatccaaag tgcttactat ttgctgcaag atgcaatcgg cgatatgagt
     7921 cgtcagattg atttattgga gttagatgag agtcgtttag aagaagttac gcaacgttta
     7981 gaattaatcc gacagttgaa acgtaaatac ggcgaatcca ttgagtcaat attggcttac
     8041 tatgacgaaa tcaccgaaga gctagcttct tctgattttt cagaaggtca attggacaaa
     8101 atgaaagagg aactagagca aaaagagctt ctacttcagc aacaagccgc agatcttcat
     8161 gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca ttctgcatga attgaaaagt
     8221 ctgtatatgg aaaacactga gtttgaggta cgtttcttaa aagaagaaaa tagacaattg
     8281 aatcatgatg gatttgacca aatcgaattt tatattacga ccaatccagg agagccactg
     8341 aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc ggatgttgct tgctttgaaa
     8401 actatttttt cttctgagca aggtgtgaca agtattattt ttgatgaagt ggatacaggt
     8461 gtgagcggaa gagttgcaca ggccatcgca gataaaattt ctcaaatatc gaaatactct
     8521 caagttttgt gcatcactca tctgcctcaa gttgctgcag tcgccgacta tcaatattat
     8581 attgtgaaag cagtgattga tggacgtacg caaacttctg tttcagagtt gaaaacaaaa
     8641 gaaagagaag aagaaattgc acgtatgctg gctggaagtg agattacaaa actcacactc
     8701 gaacatgcaa aagaactatt gaaactcgca aaaaggacct gagccacctc gcagtggttc
     8761 aggtcctttt cagcagctga tagctatatt ttatttttac atgacactca ttacagcagt
     8821 caatacaaca gatccaatcg ttaaaatcag cattgtccaa atgacaaatt ttgttacttt
     8881 agcaaaagta cttgtttgtt ttttttcacg cattgttttc aacctcgtca ttaattagtt
     8941 gattcaagtg tacaatcttt tttccatttc cacaagaggg cttttttata aaaacttccc
     9001 tttttttaaa taataggtag ttacgataca aataaataat gttaatagaa ctagccatat
     9061 aaaggcatca tccctttcag caataggtaa cttaatgttc attccataga tactgccgac
     9121 gattgtcggt atagttaaaa taatcgttaa agaagttaaa attttcataa tattattcag
     9181 attattcgat acgattgctg aaaaagtctg attcatttgt tctactagtt ttaattgaat
     9241 gcgagaagta gtcattgcct gctctgtttc caccaataca tcacgtaaat gatttttcaa
     9301 agcatgattt tcttggaaaa gtttattttg cgctagttct ttcaatgcat ctaaattagc
     9361 agaagtcgct tctctgaaat aaacgaggct tttttggata tccatgattt gataaagttg
     9421 tttgttttca gtggatactt gaagtttggc ttccaaagta tcgcattgag attgaagcgt
     9481 agctaaataa ctgttgtaag agagtgtgac ctgccaaatg aattgaaaga agatattgag
     9541 attaggcgaa atatcacttt ttggaaaggt ctggctaaag actttcttta aaaacagtgg
     9601 ttcatgattc gtaattgtga taagcttctt tttgggcgtc aaaataatag agagcgggta
     9661 tgtatccact tgaaaataac cgctaggact tgttttagca aaaggatatt gtaacaataa
     9721 taacgctggc tttgtcaact ttgattgatt gaattcttct gtacgggaat tttctgcatc
     9781 gtccaatatg gaagtgatgt aatctgacgg aatttcaaat tgatgtgtca gctgatgaat
     9841 ctcttcttga ttaggacgtt ctacacaata ccagatcgtt tggtcagttt cctttccggt
     9901 attacgcagt ttctcattat ctaatgtaaa atatttgatc atgattttcc tcctgttgcc
     9961 tgtttctttt attataacgg atagggaatt ggaatacatg gaatgtgttt gtaagatata
    10021 gaatataact gcataaattt aaataaaatg aaacagttgt gtaaagtata tacagtatac
    10081 ttgaagcaac gataaaaaag gagtttacgt tcatgggcac attcacacca cttatcttgt
    10141 tttggtatat ttacccggta atcgttttgt ttggttgcca atttcttgta aaattatttt
    10201 cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc gattcctttt ttgtggggcg
    10261 gtatgcatgc attgtctaga aatacaggga caatttctat cttaccgttt ttctttattt
    10321 ctgttttgct aatgggaatt tgtattgctg ttttccaagc atattattat gaagaaatta
    10381 tttatccgcg ttatttcaag atgacttggc gtttggtttt tctgttgaca atcgtcttgt
    10441 acgctgtctt gatcattgtg aacatacttt cttacctatg aaaaaaatag aaaagctgta
    10501 aaaagtcttt taaaaggctt tttgcagctt gatttttaaa aaaatgttta tgaataaaaa
    10561 aagttgttcc cggatttccc agaaaaggtg gtagaaagtg ggggattgtg gtagactaat
    10621 tttagttagt ggaggaatgg ggggctttca atgttgatgg gtgaattcca acataatatt
    10681 gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg aagaacttgg cgaaaagttt
    10741 gttctgacac gcggattgga tggctgtctt ttcggctatc cgatgagcga atgggaaaat
    10801 ctggaagcta aactgaacga aatgccgttg gctaaaaaag acgcacgtac ttttgtgcga
    10861 ttcttctatt cggctgccac tgaatgcgaa cttgataaac aaggacgaat caatataccc
    10921 agcacgttaa gaaattatgc agctttgaca aaaggttgcg tagtcatcgg tgtatctaac
    10981 cgaattgaaa tttgggatga gacacgatgg caagattttt ctgcagcagc agaagagaac
    11041 tttgacgaaa ttgctgaaag tatgattgat ttcggactat aagaatagga agaggctgac
    11101 taatgactga aacatttcaa cactatacag tcatgttgaa agaaacggta gatggattga
    11161 acatcaaacc ggatggcgtt tatgtagact gtacactcgg cggtgccgga catagtgaat
    11221 atttattaag ccaactaagt tctaaagggc atctgtatgc gtttgaccaa gatcaaaaag
    11281 caatcgatca cgccaagctg cgtttagctt cttatgtcga acaaggtatg gtcacattta
    11341 tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga gcatgtagat aaggtagacg
    11401 gcattttgta tgatctaggg gtttcctctc ctcagctaga tgaagcagaa cgtggattta
    11461 gttatcatca agatgcaccg ttggatatgc gcatggatca gtcagctcct ttatccgctt
    11521 atcacgtaat taatgaatat tcctaccatg aattagtaaa gattttcttc cgctatggcg
    11581 aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg agtacgaaaa acaaaaccaa
    11641 tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt catcccagct cccgcaagaa
    11701 gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt gcggattgca gtaaatgacg
    11761 aactcggtgc cgaagaggcc tctttagaac aggcaatccg gttattgaaa gtcaatggaa
    11821 gaatcagcgt aatcacattc cattcattgg aagacagaat tgtcaaaagt atgtttaaag
    11881 aatatagtac ggtacaagat ctaccaccgg gacttcctgt catacctgac gaattccaac
    11941 cagaactgaa agtcattact cgtaaaccaa tccttccttc agaggaagag ctaacagaaa
    12001 acaatcgatc acgcagtgca aagttacgta ttgcagaaaa aataagaatg aaagaggagt
    12061 agaatgatgg cagaattaaa gaaaatgcaa gattacgaat atgatttacc tgaattggtg
    12121 gatcagccgg aacaacaaat gcctgattct tcttcgaatc aagaaatcat tcttccacaa
    12181 tcaccggcaa aacgattaaa gcagatttct gctttggaaa aaattgctgt ggcaagcgtg
    12241 gcgattgcta ttgttgcttt atgtgtttta acagttatgc tgcgcacaaa tatcagcgga
    12301 gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg ataaaaagca agaaaaaacg
    12361 agtttagaac aagaaaaaaa cgaactttct cgtactgaac ggattaaaca aatcgcagaa
    12421 gaaaaaggat tgtctattaa tgacgacaat ttaaggataa ggaaagtgaa gtaaatgagt
    12481 cttaaaaata aaattcggcg gtttatggaa aagaaaaatc taaatccgat gaacaatcgc
    12541 aaaaaagtag gtatcatttt atttgctacg agtattggat tgttcttttt atttgccgtc
    12601 cgtttctcgt atatcgttat tggaggtcat gttgctggta cctcattagc tgaaaaaaca
    12661 aaacagttgt accaaggaag tgaagtagta aaagcaaaaa gaggaacgat ttatgatcgt
    12721 aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat actctcaaaa
    12781 acatatacct ctggggataa aaaactgtat gttgaagaaa agaattttga taaaatagca
    12841 gaaatcctgc ataagaatct ttctatagac aaaaaagatg cactgaatat tttagaggac
    12901 ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct atgggaaaaa tatctcccaa
    12961 gaaaccaagc aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg tctttatttt
    13021 gtcgaccatc aggctcggat gtatccaaat ggcgtctttt cttctcattt tattggatac
    13081 gcagttccag acaaagatga gaatggctta gttggtaaac taggtttgga gtctgcttac
    13141 aatgatatct tgagcggtaa agatggtaag attatttatc aaaaagataa ttttcaaaat
    13201 cctctgccag gtacagtggc agaagaagaa aaagctgtcg acggacaaga tatctacacg
    13261 actttagaca gcagactaca aagttattta gaaacactga tggatcaagt aaatgaagaa
    13321 tatcagccag aagaattgac agcagtcctg atgaaagcaa aaaccggaga aatcttggca
    13381 atgggacaac gacctacttt caatcctgaa acaatggaag gattgactgg aaaagatgct
    13441 atctggcgta atttcttggt acaagatagt tatgaaccag gatcaaccat gaaagttttc
    13501 actactgcag ctgctatcga agaaggagaa ttcaacgaga atgaaacctt ccaatctgga
    13561 aaaatccaag tagaagatgc tacgatcaat gaccatgact ttggtgaaaa aggtgtgctg
    13621 accatgcgtc aagctctttc atggtcaagt aacgtcggga tggtcatact ggaacagcga
    13681 ttaggcggac gatggtataa ctatttacaa aaactgggat ttggacaaag tactcattct
    13741 ggattagatg atgaagtaaa cggtgcttta cctacattaa atattgtcga ccgagcaatg
    13801 agtgcttatg gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc tttcacttct
    13861 attgcgaata acggtacaat gattcaacct cgatatatca gcaaagtggt ggatccccaa
    13921 actggagaag agcgaacaac tcaaacagaa gttctagggc aacctttttc aaaagaaaca
    13981 acagaaaaag tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta cggtagtgct
    14041 tatggtgtat atagtgtacc tggatacaat gtatcggcaa aaaccggaac agcccaaatc
    14101 gcttctgata cagggggata tcaaacagga gatacagcct acctgtattc gattgtggag
    14161 atggttcctt ctgaagatcc agactatgtc ttgtatctaa caatgaaaca ccctaagaca
    14221 tatgatcgaa tggcattggc taaaatcgca aatccattga tgaaacgggc aatggatttt
    14281 aaagaaactg aagaagattc agatacagaa acaaaaacag aaaaagtatc cgtagccgat
    14341 tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc aaaaaagtgg actgcaacct
    14401 gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta ctgccaacgg tgatcagttg
    14461 atctctggag aaaaactgat cctatatact ggtggtgata agctgatgcc tgacgtaaca
    14521 ggatggtcaa aagcagatat catgaagctg gggaaaatcc tcggtattga agttagtttt
    14581 gatggagatg gttattgtgt caaacaagaa cttgctccat atgaaaaaat cactgaagac
    14641 aaacttagtt ttactttaga agaataagaa gagaaatagg agagaaaaga gcatggaatg
    14701 gacacaagca ttgattccca ttgtcagcag ttgtgcaatg acaattgccg caatgccgct
    14761 atttattggt tattttcaaa tgaaaaagca aggtcaggcg attcgtgagg agggaccaaa
    14821 gtggcacaat agcaaagccg gaactcctac aatgggcggc ttagtgtttc tgatcggctc
    14881 tattttgaca ggaatttggg ttggagcttg gcaaaaacag ctaactccta ctcttttcat
    14941 cttgcttttt gtattagcgt tatacggagt aatcggcttt cttgatgatt ttataaaaat
    15001 attcaagaaa agaaacatgg gactgaactc gaagcagaaa ttgattggac agattctagg
    15061 gggaatcatc ttttatcttg tttaccgttc tgaaggttat ccaggtgtat tgaatttctt
    15121 cggactagat ctacctttag gaatcattta tggtattttc gctctcttct ggcttgttgg
    15181 attttcaaat gcagtaaatc tgacagatgg catcgatgga ttagttgctg gcttaggaag
    15241 tatctctttt gcagcttacg gtctaattgc ttggcatcag catcagtatg atgtattaat
    15301 catttgctta agtgttttag gaggtttact gggtttcttc gtctacaaca gaaaacctgc
    15361 caagattttt atgggagatg tcggttcatt agcgttgggc ggacttttag cggcaatttc
    15421 tataatgcta aaccaagaat ggacattgct tttgattggt ttgatctatg ttatggaaac
    15481 agcaagtgtg atgcttcaag ttacttcctt taagcttaca ggaaaacgaa tttttaaaat
    15541 gtctccaatt catcatcact ttgaaatgtg cggatggtcg gagtggaaga ttgatacagt
    15601 tttttggttg atcagcattg tgacttcgtt gatcacatta tggatcgttt ggtaggagat
    15661 gacgggaaat gaaaaaaata tcaacatatg aaaataagaa agtgcttgtc ttaggactcg
    15721 caaaaagcgg agtaagtgcg gccaaattat tacatgaatt gggcgcactt gtcaccgtta
    15781 atgatggaaa accttttgac gaaaatccgg aagcacaaga gctgctttct ttagggataa
    15841 aagtaataac aggtagtcat ccaatcgaat tgttggatga agaattttca ttgatggtca
    15901 aaaatccagg gattccctac agtcatcctt tagtagcaaa agcccaagaa atgggtattc
    15961 cggtaattac ggaagttgag ctagcttacg aagtagcgga atgcccaatt attgggatta
    16021 caggaacgaa cggaaaaaca acgacaacaa cgatgacagg actactactg aatgcaggag
    16081 cagatcaagg aattgcacgt ttagcgggta atattgggta tccagcaagt ggtgtagcgc
    16141 aagaagcaaa atcagaagat aaaattgtga tggaattgtc tagcttccaa ctcatgggaa
    16201 tcacagattt ccgccctcat atagcggtga ttaccaatat ctatgaagca catattgatt
    16261 atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa ccttcaaaaa aacatgacag
    16321 aaaaagatta tctgatcctt aactggaatc agtcagaact acaagaatta gcgcaaagaa
    16381 caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt ggaagacggt gtgtatgctg
    16441 acgattactc aatatactat aaaaaagaaa aaataatgga aatatctgaa cttggcgtac
    16501 caggtaagca taatgtagaa aatgcactag ctgctatttc tgtagcaaaa ttgtatggta
    16561 tatcaaatga agctatacga gaaacgttgc attttttcca tggcgtacca catcgtacgc
    16621 aatatgtagg agaaatccaa ggcagaaaat tttataatga ttcaaaagca accaatattt
    16681 tagcgactaa aatggctcta agtggatttg aaacgtctaa ggtcgtgtta ttggctggcg
    16741 gattagacag aggaaataca tttgatgaat taattccttc attaaaagga atcaaagcaa
    16801 tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc tggaaaaaaa gcaggcatcg
    16861 agacaatagt aacagccgat tctgtagaac aagcagttcc tttagcatta gaaaacagta
    16921 cagatggtga tgtagtttta ttatcaccag cgaatgccag ctgggatcaa tacccaaatt
    16981 ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg attaaaatag aaaaagtaga
    17041 agcgagcgat aaaaaatgaa aattttggtt acaggcggag gaacaggggg acatatttat
    17101 ccagctcttg cctttgtcaa ttatgtaaag actcaagaac cgaatgctga attcatgtat
    17161 gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg atacaggaat gcctttccat
    17221 accttggaga ttcaaggatt taaacgaaaa atttccatgc ataacatcaa gacggtccga
    17281 ttgtttctta agagtatccg tcaagcaaaa aaaatcctca gtgattttca accagatatc
    17341 gttatcggta ctggaggata tgtttccgga gcggtagtgt acgctgcagc caaaatgggt
    17401 attccaacag ttatccatga acaaaacagt gtaccaggtg ttaccaataa atttttaagc
    17461 cgatatgtgg atcggatcgc tctttcattt aaagatgcgg cttccttttt tcctgaaaac
    17521 aaagcagttc ttgtaggtaa tcctcgagca caagaagtag cagatactaa aaaatcagat
    17581 attttgaaaa catttgattt ggatccagaa aagaaaacag tcttgatttt tggcggtagt
    17641 caaggagcac ttaaaatcaa tcaagcagta tcagtttttt taagtgaata tattccttct
    17701 tcttaccaag ttttgtatgc ttcaggagat agatattatg aagagatcct aaataaaata
    17761 aatcctgaaa tacaaaatat tagtgtccag ccttatatca aaaatatggc agaggtcatg
    17821 gcttccagtg atctgctggt ggggcgtgct ggtgcaactt cgatcgcaga attcacagct
    17881 ctggggttac cggcagtgtt ggttcccagc ccatatgtta cgaatgatca tcagactaaa
    17941 aatgcaatga gtcttgttca cgcaggagca gcaaaaatga ttgcagataa tgaactaaca
    18001 ggtgaatcac taagtcagac agtcaacgaa atcatgggtg atgaagaatt gcaaaagcaa
    18061 atgtgccgag cttctaaaga acaaggaatc cctgatgctt caaaacgtct ttatgactta
    18121 gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt gagtaagaaa aaagaaccaa
    18181 aaaaattgtc tcatccgtct tctgatgaaa acaatttgac tccttggcaa aaggctaacc
    18241 gaaaatatct ggcagaacaa gagatgaaaa accaagagcc aaaaaaagaa gacataacat
    18301 tagatacacc cctttcagat tctgaagaag aaaaaacggt tcaagaggaa cagcaagaag
    18361 aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat caagcaaaag aaaaacggtg
    18421 gaccatataa cggttctttt ttgaaccggc tgcccaattt aaaaagtcaa cggcacaaag
    18481 tactatttcg tcggttagct ttgatcatca ctgtattggg tataccgctg atttttttga
    18541 tctattatgt ttctccttat agcaaattac aagcagttgc agtatcaggc aataagacag
    18601 tcaacagcca agaaatcatc tctgatacaa aattgtcatt aggagaaaat gtttggggac
    18661 agtattttca tcgatctact tatatcgatc gccttaaaaa agcacagcca agaatcgaaa
    18721 cagcaaatat acatttcaaa gggatgaatg aattcgattt ggatgtaaca gaatataaag
    18781 agattgcttt gatcgcaaaa aacaatcaat actacccagt gattgaaaat ggaacagtat
    18841 tagatgaaaa agtagcaaat cctacaaaaa atctccctat cttggaagag ttcaaagact
    18901 ctgcaaaaat caaagaatta acaaagcaat acaatcagtt atcatcagaa ttacaaaaag
    18961 caatttctga aatcaagtac acgccaagag caagcaataa aaatctgatc caattgaata
    19021 tgaatgacgg caatcaagtt attgtaaata tcaataatct tgcgaaccaa atgaaatatt
    19081 attcgcaagt agccaaagat atggatgaaa aaggtgtgat cgacatggaa gttggtatct
    19141 tctcgcatcc ttacggtagc actcaagaaa cggaagaatc gggccaatcg gaagaaacaa
    19201 ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag tgaagaggca gaaacgcaaa
    19261 aagcacaaac acctggatcg tctagcacaa acggatcatc tgattcaaat gattcaagtg
    19321 aaacaagtca aatagaagat aatccaagta caactccgtc aagtttaaat gaaaattgat
    19381 aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc agtaatttat ggtagaattg
    19441 gaagcagtga atatatctat taataagtat attttaaaac ggacgctagt tgttcgcttg
    19501 tatatggaat agtaagtagg agggaatccc attcatggca aaaacaggaa tgtatgtagg
    19561 ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct gaatatatcg acagccaaat
    19621 gaatattatt ggagtaggaa acgcaaaatc agaagggatt aaccgaggca ttatcgttga
    19681 tatcgataaa acggtccaag cgatacaacg agcagtacga caagcagaag aaaaagcagg
    19741 tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac atgcttgaag tagaaaattg
    19801 ccagggaatg attgcagtta acggagattc aaaggaaatc accgatgaag atgtcagaaa
    19861 tgtggcttca gctgcccttg ttcgttcaat acctcctgaa cgtcaaatcg tgtcaatatt
    19921 gccacaagat tttactgtcg atgggttcga gggaatcaaa gatcctcgtg gaatgatcgg
    19981 cgtaagatta gaaatgtacg gtctgttgtt tacaggacct aaaacgatca tccataacat
    20041 acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa atggtgatta ctcctttagc
    20101 gctgactgaa tcaattcttt ctgacggaga aaaagatttt ggtacgatcg tcattgatat
    20161 gggtggcggc caaacaacaa cggctgttat gcatgataag caattgaagt ttaccaatct
    20221 tgaccaagaa ggtggcgaat ttgtcactaa agatatctct attgtattaa atacgtcatt
    20281 taacaatgca gaagcgttga aaataaacta cggggatgca tacccagaac ggacatcacc
    20341 agatgaagaa tttccagtag atgtgattgg tcagtcagaa ccagtgaaag tcgatgaacg
    20401 ctacttgtca gaaattattt cagctcgaat ggaacagatt ttcaacaaag caaaagatgc
    20461 attggaccaa atcgaagcat tagagttgcc aggtggcgtc gtattgacag gcggagctgc
    20521 tagtcttcct ggtgttgtgg acttggcaca agaaatcttc ggcgtaaatg taaaattgta
    20581 tgttccaaat cacatggggc tgagaaaccc ggtatttaca aatgtaataa gtatcgtgga
    20641 ttattcagca aacttgagtg aagtgtatca attggcaaaa attgctgtta cgggtgaaac
    20701 ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca aacacatatg agagctatga
    20761 agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa gagtccggag aaaatgtaaa
    20821 aaataaaatc aagggtttct ttacaaatat ttttgattaa ttcatcctgg gaggaataag
    20881 atatggaatt ctcaattgat aataatatca atgacggcgc agtaatcaaa gtcatcggag
    20941 tcggtggtgg cggtggaaat gctgtaaacc gaatgattga agaaaacgtt aaaggtgttg
    21001 aattcattac agccaacaca gacgtgcaag cattaaaaaa ttcaaaagct gaaacagtta
    21061 tccaattagg ccccaaatat actcgtggtt taggtgctgg ttctcagcca gaagttggac
    21121 aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc tttagacggt gcagatatga
    21181 ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg tgctgcacca atcgttgcag
    21241 gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt gacacgtcca ttcacttttg
    21301 aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc tcgtttgaaa gaaaatgtgg
    21361 atacattatt aattatctca aataaccgtt tattagaagt tgtagataaa aagacaccga
    21421 tgcttgaagc tttccgtgaa gcagataatg ttttacgaca aggtgtacaa ggtatctctg
    21481 atttgatcac agcgccaggt tacgttaacc ttgactttgc tgacgtgaaa acagtgatgg
    21541 aaaaccaagg tacagcattg atggggatcg gtgtggctag tggagaagat cgcgtagtag
    21601 aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac atctattgat ggagctgaac
    21661 aagtacttct taacatcaca ggcggtttgg atatgacatt gttcgaagca caagatgctt
    21721 cagatatcgt agcaaatgca gcaactggcg atgtaaatat cattctaggt acatctatca
    21781 atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc aacaggtatc gatgaatcaa
    21841 aaaaggaacg caagtctagc cgaccagcta gacaagcaca aatgcaatca cctgctcaaa
    21901 agactgtatt agatatggat caagctaaac caacttcttc agaagaagaa aacagttttg
    21961 gcgattggga tatcagacgt gaacaaaatg ttcgtcctag agttgatgat tccaattttg
    22021 acaatatcga aaaaaaagaa tttgatacat tcaaccgcga agaaacaaaa tctcatggcg
    22081 atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa ataaaggaga ggaagagcac
    22141 atgattgctg acaacttgca gaaaatcaag caagaaattc aggattcgtg tgctcttgtt
    22201 catcgtgatc cgaaagaagt tacattagta gctgtaacaa aatctgttga atccgctcaa
    22261 gctgaagaat tggttagagc tggcgttatg aattgtgcag aaaaccgagt agataaatta
    22321 ttagaaaaga aacaagattt atttgatttt cctgaaataa agtggcattt aattggtaat
    22381 ttacagcgca gaaaggtaaa attgattgta aatgaaattg attactttca tgcgttggac
    22441 agtttaagat tagcagaaga aatccagaag cggttagaga aagaattatc ctgttttatt
    22501 gaagtaaatg tttctggtga ggaatcaaaa catggaattt caccagatga gcttttaccg
    22561 tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg ttgggttgat gactatggcg
    22621 cctaaagaag cttctgatga gcaaatccat aaagtgtttg gtacgctgaa agaattgcgt
    22681 gatcttgttc aagcaaaagg ttatgcccat gcaccatgta gtgaattaag tatggggatg
    22741 agtcaagact ttcaaattgc gattgaagaa ggtgcaacat ttatccgtgt aggaacagcg
    22801 ctattcaaag acgaataaag gaggaacaaa aatgccgctt ttaaacagag gagcaattgc
    22861 taattttttc ggattagctg atgaggaaga gtacgaatac caagattatc ctgaaacaac
    22921 aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag aatcaatcaa ctcattctgt
    22981 atctgctgcg aatactcatg aaaaacaaaa aacggcgact tcacgtcctg taacgcatga
    23041 aagtattcag ccggctgaaa agaaaaaagt aattgaacta catcaatcac ctacaaaaac
    23101 tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa cctaaagcaa atcttgctgg
    23161 aaaaatcact gtaatggaac caagagctta ttctgaagct atggcgattg ctaaacgaat
    23221 cattgctgga gaagcaacct tagtgaattt tcatttagta gaagaaaaac aagcgagaag
    23281 aatcgttgat tttctaacag gaacagttta tgcactagat ggagatatca aacgtgttgg
    23341 agatgagatt tttctttgta caccgactgg gatggagatt gacagttcaa ccgctcaatc
    23401 ttttgcagat gcgaatttgt ttgatctgta agaggaggaa ctacgattta tactattgca
    23461 ctattacttc atttacttca acgggcagcc tatttctata gcattatctt gatggtatat
    23521 gcattgctct cttggtttcc aggcgggtat caatcagggc taggacgatt attagctaag
    23581 atctgcgaac cgtatatcag tttgttcgat cgtctgcctt tgaggattgg acccttggat
    23641 ttcacaattt taggggcaat cctgatcttg aacttagccg tacaaggttt agcacagatc
    23701 gttatatgga taactgtcca tttagtttat tgattgaagc aacaaaatct ttattctcag
    23761 aatagatagg aggggaaaat tttggatgca aatgtatacc agcactttcg ggcagatgaa
    23821 cgtacattta tcgattcggt aggcgattgg attgaacagg ttcaaggaca atatgcaccc
    23881 tatctgaccg aatttcttga cccaagacaa tcctatattt tagaaacact gattcgacaa
    23941 agcagtgatt tgcgtttcat gttttatggc ggatatgaac aggcagaaag aaaaagatgt
    24001 ttgattttcc ccgattatta cgaacctgta gaggaagatt ttgaaacagc attgtatgaa
    24061 atccattatc ctagcaaatt tgccagccta agtcatggga aaatccttgg aactttagtg
    24121 ggaacgggga tcaaacgctc ttactttggc gatatcatct cggacggtga tcgctggcaa
    24181 gtatttattg caaatgaggt ggatcatttt gttgctgcac aagtaacaaa gattggaaag
    24241 gttgctgtac gcttggaacc aatcaaatac acagaaatga ttatgccgaa agatagctgg
    24301 acacaagaaa gaggaacagt tagttccatg cgactagaca gtgtgatctc tgagatttat
    24361 aatatttcca gacagcggtc caagcagctg atcgaagctg gtaaagtaaa agtgaactgg
    24421 acagaaaata caagacctga ttttgtactt gaactattag atattatttc agtcagaggt
    24481 tatggcagag tccagatcca agaacttgag gggaagacga aaaaagaaaa gtacagggta
    24541 ctgttcggtg tattacgtaa ataaaataaa aaggtggttt actcatggct ttaactccat
    24601 tagatatcca aaataagaca ttcccgacaa aaatgcgggg atataatcaa gacgaagtag
    24661 atgatttctt agatttagtt gttcgtgact atgaggaatt gactcaaaga aatcgagaat
    24721 tagaaaaagc agtcaaacat tctgaagaaa aacttgagta cttcaatgaa ttaaaagatg
    24781 ctttgaacca atcaatcatc gtagctcaag atacggctga taaagtaaaa acaagtgcaa
    24841 gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa agcagatgag ttagttgcga
    24901 acgctgaaaa acgtgcacat caattaacaa ctgatgcaga agaaaaagca agaaaaatct
    24961 tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga aactgaagat cttaagaaaa
    25021 aaacacgagt attccatcaa cgtatcagct tgatgcttga gtctcaatta gaacaagtga
    25081 aaagtccgga atgggatgaa attttacaac cattctccag ctacgtgact gacagccatg
    25141 aagtgatcaa agaagtttta gcaaaacagc ttgacaatga aaatgatact gatgtaaact
    25201 cagacacagg agtcatagaa gcacctatga cggcatttga tacacaggct attgatatct
    25261 cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa agcactttgt tattcttaaa
    25321 gcgagccgat gagagtggaa gttcggtaag acactgcatg gtgaaagatc ctctacttga
    25381 gacctgcttt tccgaaatta gagtaagaag cagcggctga cctcgttatt ggtcacagag
    25441 agtttgcact agatagatgc aactaaattt gggtggtacc acggcatgtt cgtcccttgc
    25501 ggctgaacat gcttttttgt gtccggaaaa caaataaagg agtgtatggt tgaatgaaaa
    25561 tgaaagaaac cttgcaatta ggaaaaacaa aattccctat gcgtggtaat ttgccaaacc
    25621 gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat ctaccaaaaa agacaagagt
    25681 tgaacgaagg aaaacctagc tttgttcttc atgatggacc tccatatgcc aatggtaata
    25741 tccatttagg acattcattg aataaaatca gtaaagacat tatcattcgt tcaaaatcaa
    25801 tgtctggcta tcgtgcacca tatgttccag gatgggatac acatggattg ccgattgaac
    25861 aagtattggc taataaaggc gtgaaacgta aagaaatgtc catggcagaa tacttggaaa
    25921 aatgtcgtga atatgcactt tcccaagtag ataagcaacg agcagatttc aaacgcctag
    25981 gagttgcagg agattgggaa catccttatg ttacattaga tccttcttat gaagctgcac
    26041 aagtacgcgt atttggtaaa atggctgaaa aaggctatat ttacaaagga ttgaaaccaa
    26101 tttactggtc tccgtcaagt gagtcttctt tagcggaagc tgaaattgaa tataaagatg
    26161 taaaatctcc atctattttt gtagctttca atgtcaaaga cggcaaagat attcttgatg
    26221 aagatacagc ttttgtcatt tggacgacaa caccatggac attgccagca aaccaaggta
    26281 ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc cgatggccgt aagtttgtcg
    26341 tagcgaaaga tttgctagaa acagtccaat cagaaatcgg ttgggaaaat gtaacagttc
    26401 taaaagaatt tgctggacaa gaaatggaat atatgactgc aagtcatcca ttctatgaac
    26461 gtgaatcact tgtgatttta ggcgatcatg ttactttaga cgctggtact ggattagtcc
    26521 atactgctcc aggacatggg gaagatgact atatcgctgg tagcaaatat aaactatctg
    26581 tagtgtcacc tgtagactca aaaggattgt ttacagaaga agcacctgga tttgaaggta
    26641 tcttttatga caaagcaaat cctatgatca ctgatctatt aaaagaaaaa ggagctttgc
    26701 tgaagctaga tttctttact catagttatc ctcacgattg gcggacgaaa aagcctgtga
    26761 tctatcgggc aactccacaa tggtttgcat ctatcgataa attccgtcag aatattttgg
    26821 atgaagtaga aaaagtagag tgggttatcc catggggtaa aacacgtttg tataatatga
    26881 tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc atggggagta ccacttccga
    26941 ttttctatgc tgaaaatgga gaagcaatca tcacaccaga aacaatcgaa catgtggcaa
    27001 aattatttgc tgaacatgga tcaattatct ggtttgaaaa agaagcgaaa gaacttcttc
    27061 ctgaaggatt tacccaccca ggttctccta atggcgaatt tacaaaagaa aaagatatca
    27121 tggatgtctg gtttgattct ggttcttctc atgaagctgt tttacgtcag cgaccagaat
    27181 tatcattccc agcagatatg tatttagaag gatcagacca atatcgtggc tggttcaact
    27241 caagtatcac gactagtgtg gctatcaatg gtgtagcacc atacaaatca gttatttctc
    27301 aaggatttgc tttagatggt gaaggccgta agatgagtaa atctttagga aacatcattg
    27361 caccagaaaa agtgatcaaa caatttggtg cagatatcct tcgcttatgg gtatctagtg
    27421 tagattatga agcagatgtt cgtgtttcta tggatatctt aagtcaagtg gcagaagtat
    27481 atcgtaaaat cagaaatacg atgcgtttct tattagcgaa tacagaagat ttcgatccta
    27541 aaaaagatac tgtttcgtac aatgatttac gttctgtaga caaatatatg accgttcgat
    27601 tgaaccaagt aatcaaagaa atccgagaag aagggtacga caaatataac ttcatgcata
    27661 tttaccgtac agttatgaac ttcttgacgg ttgatctatc gtcattctac ctggattttg
    27721 ctaaagatgt agtttatatt gaagcagaag atgattatca acgtcgttgc atgcaaactg
    27781 ttttctatca aacagctgta gctttgacaa aattattgac tcctatcatt ccgcatacag
    27841 cagaagaaat ctggtcattc ctgaaagaag aagaggagta cgttcagtta agcgagtttc
    27901 ctggttatga agagtttgca aaccaagaag aattgatgga tacctggact gctttcatgg
    27961 acttccgtga taatgtctta aaagcattgg aagaagcaag aaacagcaaa ttgattggta
    28021 aatctttgga agcaaaacta actgtttatc caaaacaaca agttcgtgaa atgttaaaag
    28081 ctttggatgc agatattgca caattattga tcgtttcacc agactacttt gaagttatgg
    28141 cagcagatga acaagtgcca gataacgcta tggtatttga tgatgttgct attacagtgg
    28201 aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt tcgtaaagat gtaggagtag
    28261 atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat cgttgaagac ttctatcctg
    28321 aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa agggggtgtg acatgttctt
    28381 tgtcacaccc ccttttgtta tcaatcgttt aattttccac gttcacgata ccagttatct
    28441 ggttgccaga tccattcgaa tccgtctttt tcaagtagtt cgaaagctgc tttaggtccc
    28501 attgatccag ctttgtagtt gggaaaatcc ggttgtgttt ggtcccaagc ttttcgaatg
    28561 acatccacga tatgccaaga ttgagcgact tcttcccaat gagagaagtt cgttccatca
    28621 ccattcaacg catctaatag taatttttcg taagcttctg ggctattttc aaccatttct
    28681 gcactatttc taaattccaa tttgattggt tccgtttcaa atccttgccc tacttctttc
    28741 ccattcaagg ataaagaaaa tccttcagtt ggctgaatat agatcgttag aacattaggt
    28801 ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt tgaatacatt aacaggaact
    28861 tgtttgaata cgatgttgat ccgagtccct ttttcagtca aacgtttgcc tgtacgtaca
    28921 tagaaaggaa cacctgacca acggaagtta tcgatagtga atttgcctgc gacgaatgtt
    28981 tcagtctctg attgagcatc cactttgtct tcttcgcgat agcccttgaa ttgtacacca
    29041 tccaattcac cttgagcata ttgcccgcga acgaagtttt ctaacacttc ttcttcgtta
    29101 taaatgcgga tagctttcaa tgctttgatc ttttctgtac ggatttcttt ttcggaaaat
    29161 gctactggcg gttccattgc aagtaatgct acaacctgta aaatatgatt ttgcaccatg
    29221 tctttaagtg cgccactatg atcgtagtag cccccgcgat cttctacacc gaggttttcg
    29281 ccaaaagtaa tttggatatt gtcgatatag cgattgttcc attgagcctc aaaaatatta
    29341 ttggcaaaac ggatagctga gatgttttgg atcatttctt ttcctaaata atgatcaatc
    29401 ctgaaaatat cttgttctgg gaatacttct ctgatttgct ggttcaattc aaaagcagac
    29461 tggtaatcag aaccgaacgg tttttctatg atcaaacgat tgaagccatt ttttgtcata
    29521 atattttgtg atttcaaatg agaaacgatc gttccaaaaa attgaggtgc catagccaaa
    29581 taatagacac ggttaccgtt taattgatag tgtccatcta atcggtcaga caattctttt
    29641 aatgtctgat aatgcgttga atcatttaca tcatgggatt gatagtaaaa atggcttgca
    29701 aaggtagttg cttcttcatc tgaaggattc aatgcacgga ttgtttcttt gacaacttca
    29761 cggtagtgct catcgctcca gggacggcga gcggttccga ttacagcaaa atgctcgcct
    29821 aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca atttacgctg ggctaggtca
    29881 cctgtaccgc cgaatatcgt aaacaataca tttttttcat tagacatgtg gatacactcc
    29941 tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa gctgacgggg attttctttc
    30001 tatcattttg gatagtgatc ggtccttcat aagctgcgat gtcaacgatc tcatattctt
    30061 cgttgatatt caggtcaata gtgactaagt aatccaacaa ttctttttca tcaagcactc
    30121 gtgcgatacg cacttttgta ccgacaggat aggactccaa tgtttgtcgg cgttcttcat
    30181 ggacaacttc ctctttttta ggaatcgatc ctccatgagg acaatgttca ggatgattca
    30241 aatagttttc taaatgatct gcaagatgtt ccgaagtcac atgttccaat acttcagcat
    30301 catcatggac ttcattccag ctatagtcta aatgttcaac taaaaaaact tcccataagc
    30361 gatgtttgcg aatcagactg ctagcttttt gcagtccgct tgcagtcaat tgtacacctt
    30421 gataagggga atgttctaca agctcttctt tcacaagttt agaaatcatt tcactgacag
    30481 atgcagctga gacatctaga ctagatacga tttgtttatt gttgactttc gttgtatctc
    30541 cacctagttc taaaatgatt ttcaaataat cttctcgatt tggtgtcata ttcgatcatc
    30601 ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa aaaacgttaa ttgactattt
    30661 ttctaaataa aaaacaaagg atttttagac gtttttacaa caaaaaacga aagatgatag
    30721 agaagactca ttctttcgtt tataaaggat atatatatac attgggaggg atctattttt
    30781 cagataacaa cttatatgaa gtgtctgcag attcgtcata aagagtttgt tcgggataag
    30841 aaaagataag ataaaaacca tactgatcac gtacgactaa acgatattca tctatttcta
    30901 ccacagatgt gccaagagac tgaccgttga tcaaaaacgt gtaatcagat gtaatctgta
    30961 attgttgttt ttgctggcta tcactaaaga accatgtacc ttgatacttt gagagtcctt
    31021 ctgacttttc cgcttgtttt ttctttttca agtaaataaa acttgagaga acggtaatga
    31081 gtgaaccaag aaaatagatc atttttttta tcaccaaatc actcctttat gagaaaataa
    31141 tcgcatagac atattctttc ttctttattt cattgtaccg aataaaagct aaaatttctc
    31201 taagactttc gactggtttt tttactgttt ttctacaaat tatgatagga tacatagtag
    31261 gaagaaaggt gacataattt gaagtatcag caagtggaaa tagctttttt tattgaccgc
    31321 ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag aaataattat tgcgcatgtc
    31381 aaaaatacag gtagaggaaa agaggtcctg ataccaggcg ctgaagtggc tattgtattt
    31441 gctcctggac ctaaacggaa aaccgcctat gatctgattg ctgtaaaaaa gaagggtgac
    31501 tggtttaata tcgatagtca attgcctaat cggctggcaa ttgatggtat tttagacggc
    31561 acgatccttt taccaggatt aactggtcca tttacggtat acaagcgtga agtaacttat
    31621 cttcactcca aatttgatat ttatttagag acggtggtcg gacaaaaagc atttgttgaa
    31681 gtcaaaggta tgacacttga aaataaagcg attggtgctt ttcctgatgc gcccacactc
    31741 agaggattga aacatatagg agaattaacg tatgccgcac aagatggtta tgccgcttat
    31801 gtcttgttta ttgctcagtt cgaacatctt catctggcaa cgatccatga agaaatgcaa
    31861 ccagcactag ctgatatggt tcgacatgcg cagcaaagcg gagttcaaat cctcgcctat
    31921 aattgtcaag tatcggcaga tgaggtaact gtcaagcagg ccattccatt tgatgtagat
    31981 atcccttttg aagatcctat acaatagaaa gaaggtaaaa taatgatcaa cttaggtatt
    32041 atcggaacaa attggattac tcatcaattt gtccaagcag ctttaagcac aaaaaaatat
    32101 gaattgacag cggtatattc tagacatttg gaaaaagcac aggaatttgg ttctcattat
    32161 gaaggggata tcgaattcgc aacagatttg aagacttttt tcaatattac tcatatggac
    32221 accatttata ttgcttctcc gaatagtctt cactttagtc aagctaagca agcaattttg
    32281 gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga cgccaaaaga aatggatgaa
    32341 atcatccatt tggcaaatga acacaatgtg tatttttttg aagcagcaag aaatatccat
    32401 gaatcaggtt ttaaaaagat tgccgattac ttaccattaa aagacgagat aatcggtgca
    32461 aatttcagct atatgaaata ttcttcgcgc tatgaccaag tattagatgg agaagaaccg
    32521 aatatcttct cgactcattt ctcaggtggt gcattgatgg atttaggtgt ttaccttgta
    32581 tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc attatttccc aagaaagatt
    32641 tctactggtg tagatggtct tggatggggg atcttacgtt atgagaattt tgatgtatcg
    32701 atccagccag gtaaaatcgg tgattcttat ttaccgtcag agatttattt tgactcagga
    32761 acgctagtga tgaatggagt caatgcaatt gaacaagcac aatttcatga tcgcgcgcat
    32821 aaagaaatca ttgatttggc ggtaacagct gcagaaaatc caatggcaga tgaagcagag
    32881 gattttgcca atgtcatggc tcatccgaaa gatccagctt ggggattgtt gtatgaagag
    32941 tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg atttaagaaa aaatggcggc
    33001 atcaaattcg atgcagataa tgaggaagat ttttaatgaa cgaatttgta caagtatttc
    33061 ccgaaaattg gcagaaaaaa tatgaggata aagggtttat ctcaccttct cttattcaac
    33121 gagcagtttt tcagccacta agtaataaac aaagtatagt agcggtttct cctacaggtt
    33181 caggtaaaac actggcttat ctttggcctc tactactaaa cgtggaacca ggtgaagcaa
    33241 gtgcgttggt catttttgct tcttctcaag aactagctat tcaagtagca gatgttgcac
    33301 gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt ggtgggcgga gccaacgtca
    33361 aacgtcagat tgagggtttg aaggaaaaac cagaaatctt gattggtaca ccaggaagaa
    33421 tattggaatt gatgaaagca aaaaaaataa aagcacatca agtgaaaaca atggtatttg
    33481 atgaagcaga ccagctattt gacgctggga acagccaaat cattgatcaa attcttcatc
    33541 aagctccaac agagtatcag ctagcttttt tttcagcgac agcagatcgt tcccttgaaa
    33601 gcattgaaaa aatcactggg aaaacgtttg agacaattga cgtgacagca gaggacgatt
    33661 ctcgaaaagg actgagacat tactttttac gtgtaccgat acgaaagaaa gatgactact
    33721 tgagacgtct cacgcatata gaagattttc atggtctgat tttctttaat caactgactg
    33781 aattaggcgt aatggaagaa aaattgctgt atagaggtgt accggtagga agtctggcct
    33841 ctgatcagaa caagctactc cgaaaagcag cgctagaaca attcaaaaag aaaatggtca
    33901 gagcactttt gaccacagat gtggcggcta gaggtttaga tattacaggt cttccttatg
    33961 tagtgaatgc agaagttccg ttaagtgaag aagcttattt gcatcgttcg ggaagaactg
    34021 gtcgaatggg taatgaagga atagtaatta cactcgtaca agataataat ctaagagatt
    34081 taaaacggct tttacgtcca tcaaatataa cacttgaaga gattttttta tacggaggta
    34141 agctgcaaac tgagcagcca accaaagaag atgcagctcc tgcagtaaat aaaaagtatg
    34201 cgaaaacttt ttctggaaag gagcagaacc cagaatataa caaagaaaaa ataagaaaaa
    34261 aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc aaggcgcaaa taagcgcctt
    34321 tttatgctat gattatgaaa acgggaacaa aattggttgc cagaggaggg gttatgtgaa
    34381 agaacgtcaa gaagagatct tgcagctatt gacagaaaac caacaaggat tgacagcaag
    34441 tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca agccgatatt taagtgaact
    34501 atacaaggcc catcatattg taaaaaccgc tggccgacca gtcgtttact ctttgccaac
    34561 ggaaaagtca aaatctgatg aagtgcatgt cgactcttct acgcaagtga cttttgaaac
    34621 actcgttgga gaaaatgatt ctttaaaagt cagcatacag caggcaaaag ctgcgattct
    34681 ttatccaccg cgggggttgc atacgattat ttttggagaa actggtacag ggaagtcgat
    34741 gtttgctgaa tgtatgtatc attttgcaat cgattcagag atgctttctg cagatgcgcc
    34801 atttgtttct ttcaattgtg cggattatgc tcaaaatccg caactgctct ttggacacat
    34861 ctttgggatc aaaaaagggg cttacacagg agcagctcaa gatagtcccg gtctaatagc
    34921 caaagcagac ggcggtatat tgtttttaga tgagatccac cgtttaccgc cggaagggca
    34981 ggaaatgctg tttactttca ttgataaagg tatttaccgt ccgcttggag aaagtgcaca
    35041 agtacatgaa gcatctgttc agatcattgg ggcgacgact gaatcttcag aaagtttctt
    35101 atctacgttc aatcgacgga ttcctatggc aatcaccttg ccgaatcttg cttcacgttc
    35161 actggatgag cgttatgaaa tcatctctct ttttatcaaa caagaagcga atcgtttgaa
    35221 tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc atgctttatg atgcggaagg
    35281 aaatatcggt caagtgaaac gagacttgaa actagtctgt gcaaaatctt ttttacatta
    35341 ccgtactcat catgagaaaa agctaatcat tcgaaaagaa gaactttccc ttcaagtaca
    35401 aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc gaccgattca tggacagcaa
    35461 aagtcagtat ttgacatttg aaccaggatt tgctgatgtt gtctggtcac aagatccaga
    35521 acgaaacatg caggtctata acgatattga ggaaaaagtc acttctcttt ccgaaaccgg
    35581 tgtggagaac attgatctag agaccctcat ttcaaaagat gtggatgctt atttccagac
    35641 atatgtcaaa gaattgacta aaagttcgat tcccaaagat cttttgccag atgaaatctg
    35701 gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg ttagaccgta tttataatga
    35761 aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca ttggatcgtg tgagagaagg
    35821 tcatatgatc gtccacccag atttgaataa cgtacggaaa aattttaaga gggaattcca
    35881 ggtagcaatc gatctttcaa cgatcattga agaagaagaa caagttgaga ttccttttga
    35941 tgaaatcggc tttattagta tgtttctttc tatcaatcta ggagaaagtg agcctgttaa
    36001 agaaaacaaa gtagatgttc tcatattgat gcacggcaga cagactgctt caagcatgtt
    36061 ggagatggca caagaactat taggaacaaa agtagggaaa gcttttaata tgccactggt
    36121 tatggaagtt caaacaatgt atgaagaagt gttacaatat gtcaaaaact atcaagagca
    36181 tttgacgaat ggcctactct tgttaacaga tatgggatca ttaaacacct tcagtgattt
    36241 gatttatgaa gagactggca ttcggacgaa agctatcacg atgacaagta ccatgattgt
    36301 gatagaagcg atacggatgg caagtgttgg aagaagctta gaagatattt atcaaaatat
    36361 ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa gcggaaaagt ctgaaacttt
    36421 acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt gaaggagtag ctgccaaatt
    36481 atatcagcgt attatgccag tgattaatca aaataaggta gaactgatcc agatgcagtt
    36541 tttagaacgt gaatcattca aaaaacacat cgatgcctta ttggaagaat atgagatcaa
    36601 agcaattgcg ggaacagttg atgttgatta tcaaaatatc ccattttttt cagcctatga
    36661 cgtttttgat gatgaaaaac taaatatttt aaagcgtata gctgatgatg agatcccgac
    36721 agctaaaatt gtcgattcac ttgctggatc attaagcgat gtcttttcta ttgatgaact
    36781 gatccgttca ttgcaaaaga cagttcagca gatccagacc gatttgtcga ttatcgtgga
    36841 acctggagta gatactggaa tcgttatcca tttagccttc atgttagacg gattgatcaa
    36901 acaagaaagc catcggcaat ttgagaaact gtctattttc tcaaaaattt atcgtttgga
    36961 aatggatatc gttcgggcaa acttgatggc actagaacga aaatatcagg taaaaattcc
    37021 agaagatgat atcgcatttc ttacacaaat gtttcttgaa aataaaataa aaaaatgaac
    37081 acactaatca tactgtttac acactaaaca tctgtgtgcg cactataaaa aagagctaaa
    37141 aagcatgaaa acgctatttc agctcttttt tttattggca tgcttcttgc gttatataaa
    37201 agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa aacaatcatg ttagtttgtt
    37261 cagcagggat gagtacgagt ttattggtca caaaaatgca aaaagcagca gaagaaaaag
    37321 ggatggaagc cgatattttt gcggtctctg cctcagatgc agataataat ttagaatcta
    37381 agaatgtcga tgtattatta ttaggtcctc aagtacgttt catgaaagct caatttgaac
    37441 aaaaacttgc gcctaaaggt attccattag atgtcatcaa tatgcaagac tatggcatga
    37501 tgaacggaga aaaagttctt gcacaagcag aaaatctaat gaaataaatc aagtatgcag
    37561 ttgggggtat aacaacgtgg aagatcaaca aaatctagaa gccatcatgg ggttaatcat
    37621 gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt gcggctgcta aaatcggtga
    37681 ttttgagtta gcggatagga aaatcgctga tgcagaagaa tcacttatac aagcgcatca
    37741 tgcacaaaca gacatgttga cacaagaagc acaaggaaat catatgcaag tgacattatt
    37801 gactgttcac agtcaagatc acttaatgac atctattgcg tttactgatt tagcaaaaga
    37861 aatcatcgaa ctttatcgtc gcgtatacag cgaataaata caacttcttt tttgaacgga
    37921 agaagaaaaa tttttgaacg gattttttat tttgttataa ggagaactct ctttatataa
    37981 aaaacttata ggaggaagta aaaaatgaac ggactatcaa gttggatgga gaaatacatc
    38041 ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag ttgctttgcg tgatgctttt
    38101 atcggcacac tgcccgcaac aatggcggga tcagtcgctg taatgatcaa tgcaattatt
    38161 cgtgatttac cgcaacagtt taacagtacc tatgctgaca gtttagcagc agacaaagga
    38221 atctttgccg tattgaatgt catcatcgga atcaatggtt ttgtctggaa cggtacacta
    38281 gcaatcgccg gattgatctt tgccttttct tgggggtata atcttgcccg agcttatgga
    38341 gtaaatgatc tagctggggg gatcgttggt ttagctacct tgattcaagg tatttccttt
    38401 ggtccagcat tatcagcagc attagatgtg aatgttcctt caaatgtcgc agatgcaatc
    38461 aatagttctg agattggtgc aacgattgca gatggcacac tgtcagttgg attatggggt
    38521 tggctaaaac tagatcattt gaatggaaac gcttatttta cagttatgat catgggagct
    38581 atttcagtca ttattttttg taaattgatg cttgccaaca ttacgatcaa aatgcctgat
    38641 tcagtaccac cagcagtatc taaagcattt gcagccattt taccagcgac gatcgcttta
    38701 tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag ctgatgggca actgatcatt
    38761 gatttggtgc aaaaatatat tgcagaacca ttcctaggat tatctcaagg gttgggagca
    38821 gtattgatcg tcacagtatt tgttcaagta ttctggttct ttggtattca tggaccaaat
    38881 gtattggcac cagtgctaga gggaatctgg ggacaagcac aactaatcaa tattgatatt
    38941 ttccaaaaag gttataacgg aaaaactggt acaccagccg tgttagatgc aatcgaagat
    39001 ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt ttgcatggtt tggcggatca
    39061 ggcggaacga tcgtcttact gatagctatc ttgattttct ctaaacgtgc agattatcta
    39121 acagtagcta aattatccat tgggcctggt atcttcaata tcaacgaacc agtgatgttt
    39181 ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat tcttgattgc tccagtagta
    39241 gcaacagcaa ttggatggac agctacttat cttggcctag tcgctccggt atcgcagcaa
    39301 gtcacatggg tagtaccgcc gttcttattg tcattcttag caacaggagc ggattggcgg
    39361 gcaccgatcg taacattagt atgtatggtc gtgactttct tgatttgggc accatttgtc
    39421 atctctgcaa acaaaatgga ccctgccgca ggcgaacaac aataagatga acgtgaggag
    39481 cgggacgctt taagtttcgt tcctcataat gaattttcga ggaggaactg aaatgattac
    39541 aattgccagt gcaatgcaac aagatgaaat caaagaattg ttagaaggat acaatgaaga
    39601 aggaatgact tttacattta aagaaaaaca aggaatcaag ctgttctttg aaacaagtgc
    39661 agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta atcaaagcac agccttgggg
    39721 aacagtcctt tattttcaat caacagctgt ttagaaaatg gtttaaaaag ataggatggt
    39781 tgagatgata aaaaacggtt tatttttatg tagtattggt tctttgatcg tgttgtatag
    39841 tttgaatcca cattcgcaaa ggtatgattt gtatagtatg gtcacaggtc tttttctaac
    39901 aggattagga atctatttct tcatgaaggg gaaaaaaaga gaagaagcaa aggagaaaaa
    39961 taactaatgg aaatcacgaa aaaattaaat attccagcat cgttttttta cgataaagtt
    40021 atggactctg tccttttcga tgtacgtaaa gcgaccggca aatcagtaac tagaaaacag
    40081 ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata accgggctcg cataaaaatc
    40141 gaagaagtca ttgaaaacca gtcttataag tttcgtacat cgacaacgaa gaatgaattc
    40201 gtcgttcatt atcaaatcaa accattggac gattcgacct gtgaggttca ttacacagaa
    40261 aaaatggagt cttatggcat gcttcaaaag ttgaatgata tgcttttagg aacaatcttg
    40321 atgtatttta aacggagacg tttcaaaaaa atgctagata tgatcgaagc aacgtattaa
    40381 atcaatgaaa catggtacta tttagttatt gaaaaatgaa gtaagctaga aggaattttt
    40441 cataggaaga tagatctttc ctgcgaatcc tcactagctt ttttgtagga aggggcagca
    40501 aaatgacaaa caaagtaaga gagctaggtg tatcttcgat tgaggaaatg tttgatttag
    40561 ccacttatgc atttaatggg gcagatacac ctgaaaggcg agaacgtttt cgtacacttg
    40621 cggagaattc ttggaattac ggattttttg atgaggagga tcggctaaca agtcaagtca
    40681 tggcgacccc ttttccagtg aatttttacg gacaagagta cctaatggcc ggcattggct
    40741 atgttgcttc ttatccagaa gcaaggggac aaggcgggat caatcagatc atggaaaaga
    40801 tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta tctggcgcca ttctcctatc
    40861 ccttctatcg gcgctatggc tatgaacaat catttgataa aatcagttat caactaaaca
    40921 gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat aaagcgtatg gattttgaag
    40981 atgcaaaagc tgcaatcaga agaatttatg aacagatgcc ggaaaaccaa cgagctggtc
    41041 tgaagcgcga agactggtgg tatacgtata aattcaagca aaaaggcaat taccagtttg
    41101 ctttgcattt tgatgataat gaggaagcaa cgggatatat cgtttatcag cttgcaacgt
    41161 cttcctttat tattgcggag tggggatttt tggatcatga tgctttttgc agccttgttc
    41221 gcttcgtaag ttctcacaat ggtgcttttg aaacatttga atattcatgc ggctacggcg
    41281 gtaatgatca aaattatcta ttagaaaatc catttgcttc tgtaatgatc acaccttata
    41341 tgatggcgag gatcgtagat atttctttgt ttttggaaaa ttatccattt aaaaagagac
    41401 aagctgcatt tgccctacaa atagaagaag atcattatgc gaaatggaat gaaggaatcg
    41461 tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa agtagaacaa acaaaactcc
    41521 caatcgttcg aggaagtatc caacatatca cgcagctatt attagggtat cgtaaagtgg
    41581 aagagttggt attccaagaa agaatacagg tgacaaaaga atatgaagaa acgttaagct
    41641 caatcttgcc acagcaacag ccaattctta atgactattt ttgataaaaa acgggtgatg
    41701 acaatatttt tgtcaccacc cttttgtttt tcatttggca tttgaaggaa aatgttaaag
    41761 taaatcctct tttttcgtaa atttgaattt tttgttttct tttgttttag ggttttcttt
    41821 tttgtggtct tggattgctt ctttcgtaat cttttcccga ttttttttag atgaatctaa
    41881 gcgttctgcg tatacttttt cttttggctt atcattcatt ggaaaggacc tccctacatt
    41941 atttcattct acctctagtt tatcatctgt taaggactag tggaaacgtt ttgcctaact
    42001 tataagctga tttttttagc agataagctt gcaagtaaaa aacaaacaag ctacaattga
    42061 tttattgaaa ttgttcctgt agctcagtag gatagagcaa tcgcctccta agcgataggt
    42121 cgctggttca attccagtca ggaacgtata aataacccct gtatcctttg cggtacaggg
    42181 gtttgttttg tatttgtgcc aaaaatgtgc caaactagtt atcattttta tataaactat
    42241 cgaacacagt attgattttt tctttatcct gatcttcaag ctctcgaaca atatgaagat
    42301 aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat
    42361 ttactccttg ataaagtaaa atagatgcat gtgtatgcct tagtccatgc aaagtaaatt
    42421 gcttttcaat ccctaattct tttaattttt tgcgcaaata tttgctgacg gcgtttgatg
    42481 aaactaatcc attttttaag ttgaaaaaaa caaaattatg agggtttttg atttcaaaat
    42541 tctcgaataa ttctttttga ttgattttaa acttttttaa gagatcaatc gtatgtttat
    42601 caatggaaat tttcctattt gaagtttcgt tttttgtttt tccccattca tttaatttat
    42661 aattccatgt tttattaata gtgatgattt gatcttcgaa atcgatatct tcccatgtta
    42721 atcctagaag ttcagcaaat cgcattccag tggcaccagc aactaacacg agcataggag
    42781 aagagtactg cgcagaaagg ttttcttcta cgagtttcat taaagattta aattgatcat
    42841 aatctaaata tttatcttct tttggcttca atgaatcttt tcctttgatt actgcttttc
    42901 tagttggatc aaacggaatt aatccttctt ctacggcatc tttcaacgac gctctaatat
    42961 ggttattaaa cttaataacg gtagatttta catggtcttt agcatactta tttaaaaagc
    43021 gttgatatcc cactctatct aaatcagaaa tcaataccgc tggcatatat ttctttatgt
    43081 tcattaacgt atcttcgtat tttctataag tgataggaga tactgttcct tctttataaa
    43141 gttgcatcca atcctcaaag taatctgata ataatagatt tttgcgatcc cctttaaggc
    43201 ctttgttcaa gttatattct aattcgttgg cggcatcttt tgcttctcct tttgtcttaa
    43261 atcctgattt tcttatcttc gtgtattttc cgtcatcttt tttgtatgaa atttcgtatt
    43321 gccaactatt tccacgtttg actaatcttg ccataattga atcagctctc tttctctgat
    43381 acaataggaa ctataaagaa gcccattgtt tgggttttat tattttttgc acatcctttt
    43441 tgcttgccgg cgcaaggatg tgctatttgt cgttttaatg gtataattgg atgtgtatta
    43501 gatcatccaa tcagaggtgt ttattatgat taaagtgatt tttaaaagaa taactgaact
    43561 tccagagttt agattatttg ttgattattg gtatgtctgg ttttttattg ttgcattgct
    43621 gataggtcta gatgtcatat tagaaagaaa acttaagtaa gagttgagtt aataaattga
    43681 attcctaaat gagatatttt gtagcccata ttttgtttgc cgacatcacc taatactatg
    43741 gagctaatcg ttttttctaa cagaccaaaa tcagataatc tatttaagct accatctatt
    43801 agctcttgtg aatagccaga catgctgata tttccagtga aattttcgtt tccacgactc
    43861 attaagcatg ctaacaattg aatctcaatc gatgttaatt gattcagtac aagaagataa
    43921 tactcctcgt tatcccatga agcgttgtta attcgaagta aacaattctt ataaagattt
    43981 ttatacatat ctattttatt ttgtgccttt gatttttcta cctcgtcatt tatcccttcg
    44041 aatattccaa tgagttggtc cttgttctct aagttgaaaa catcttctgg aatttgatcg
    44101 cgaatgtttt ctaatgattc gtttaaattt ttgtagaatt tctcgatgcg tttgaaacgc
    44161 ttctcgtttt tagacccaaa ataaagtgac tgcacaacgc caccaatata aggaattgct
    44221 ccaataccgc cttcaactat taattcaaga tattcttttt tggttagttt ttcattgcta
    44281 ttatccatag ttacctccta ttttgtcatt tcctttacaa gcattgtgac tcctgatggc
    44341 actcttttgt ataggtttta ttattgtgca caatctaact cttagtgggg cgtggttcat
    44401 ttatttttta cttactttac tattaatcat atcccattga gcataggatt tttctatgtt
    44461 cgatcctctt gaactaattt ctcctgtaaa agttacttta tcgccctttg taaaggtatc
    44521 agcattgttt aagtccttcg caaaaattac atatgaaact ttaggtgttg cagaaatatc
    44581 attccaactt ttatcatcgt actctttgtc tgctataatg gctattctag tagtcattga
    44641 ttcgataaca gttccttcaa aagtatatgt tttaccacta atatatttag taaaagctgt
    44701 attttgctca gtagctggag taatagaata ataactatct aagaaagcac cccagtcatg
    44761 attatttgtc tctaaaaatt cttcaaattg agttaggctt ttattgctgc tgttagatgt
    44821 agtagtttga gtatcagatg atgagctttc ctgtttgtta ttgtcatttg cctcgtttga
    44881 tgaggaaagc gagaaaccta cggtagtaaa aataatcccg ataataagga gaatcatagg
    44941 tgttttctta tttgtttttt tgataaatct tacaattaac atgattaaac ctataataaa
    45001 taaaacaata ccagtaaaac caaatatacc gtccattaat agatcctctt tctcttttga
    45061 tattgaatat tataaaccaa gcaattcttt tttcttaaaa tcgaattctt cttgattaat
    45121 aatcccttca tctaacaact ctttatactt tttaatttca tctgcacttg aaatttgtgt
    45181 ttctttggaa ttattatcta atatagattg ttgcttctct atatattttt ttaaattttc
    45241 catttcattc caatattttt ttgtaaacat aacggtgttt tcatctttcg tggccgctaa
    45301 tatacctccg cgactttcgt ttccgcctag caaggtaaat tgtatataac cattactcat
    45361 acctggtttt tttaattgta cggctgatat atttttaaag gggatagttt tttctccttt
    45421 aataccttga ttaactaaat ttataaatcc ttttctaata attgaaatag aatcatcagt
    45481 tactttaatc aaggtttttc ctggagattt tattaaaatc tctttttcca ttttttcttc
    45541 ctcatttctg tgatatgatt ttttttgtag aagctcatat atgaggaaag agcccgtgtt
    45601 gcagcacggg cttttttaat gttttgctga aatcggtttt tttaataatt cttgatgtat
    45661 tcatacatat tgccttgcgt aagaatattt tttcttaaaa tagcattggc agacagcata
    45721 acaagtgtat ctgagcttat tctgaataga ataatactcc ataaattttt cgagattaaa
    45781 ctgagattca tcagtcagtt cgttctcaat atagatattt aataaaatta gaatagctat
    45841 tttatctgct tctgtttcaa attttgagtg aaaagttgta gaagtatcgt acaaaactga
    45901 aaattcaaaa tgagaggcgc tgaaatgtgc aagttcatga gataaatgaa aggcttctgc
    45961 agtttcaccg tatagatttt cattcaaaaa aatgattctg ggttttggat agtagaaacc
    46021 aggctctttc atctccatat aaactacttt caaattgtat tcgctcaaca tttctttcaa
    46081 tttcaaatac atacaaacca tcactccaac tattcatttt cctctaaagc tttagcaatt
    46141 gcaatcgctt tacgcattgt ctccttagat atttcttttc catcaaaaga aaaaacagta
    46201 tcgtcttctg ataaatccac atgtttaggg gtttctcttt cttctctacc tagaaggtag
    46261 tctacagaga catcgaaata gtcagcaatc cgacttaatt catctgaatt aggagtatta
    46321 tttttccact ttgctagata accgttagaa tatccaaaat taatttctaa ctgccgtata
    46381 gatatctttt tcctcttcgc caagtctttt attatttcat aggtattcat cgataaaaca
    46441 acctttctaa atgtttacaa aaaaagttta gaaaaatacg cagaaataat ttgactaatt
    46501 ctgagtaatg agctatacta tatcttgtaa acagatttaa caactaaaaa gacaacaaaa
    46561 aacactattg atatgtaaat gcagaccgcc aagaaagctt taaaatcaat gtttttatgt
    46621 cttatttaat tatgctctta gtatagagta ttactcagat actgtcaatt gaatttagaa
    46681 aaaagttgtt aaatttgttt acgaatataa aagaaaggaa tgagaaatat gaacacaccg
    46741 caaattttta atttcgaaca aaacgaagtt cggacaattt tagtaaatga tgaaccatat
    46801 tttgtaggca aagacgttgc gagtgttttg ggttattcaa acactaaaga tgctttgtcg
    46861 cggcatgtag atttggaaga taagatgggg tcgcgaatca cgacctcagg tcaatcaaga
    46921 gagatgacaa tcatcaacga atctggtttg tacagtttaa tcttaaaatc aaaacttccc
    46981 tctgccaaaa aatttaaacg ttgggtaaca agtgaagtgt tgccagcaat tagaaaacat
    47041 ggaggttatc taactccaga aaaagtagaa gaagctttgc ttaatccaga tacaatcatt
    47101 caattagcaa ctcaactaaa agaagaaaga actggaagat taatcgcaga acaaaagatt
    47161 gcagagtacg aacctaaaat ctcctattta gatagcatat tatcttctac agattcagta
    47221 acaattagtc agattgcagc agattatggg atgtctccac aacagatgaa taaattactt
    47281 cataaactag gtattcagaa aaaagtcggt aaccaatggt tattgtgcaa aaaacacatg
    47341 aaccaaggat acacaaaatc tcatacaact gagatcccga aagccgatgg tggcactaaa
    47401 attgtaatga ataccaaatg gacacagaaa gggcgtctat ttatctacga attactaaaa
    47461 aaagaaggat attaccctca aatggattta gaggaaattg gttagaaagg agttttagta
    47521 tgactgacat tgcagaaatc actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa
    47581 gttcaaagtt cttaacttac accatgcttg ctgaaagatt tggaattagc aaaagctact
    47641 tatctttaat tttaaacggt aaaaagactt ctgcagaagc aaacagaatt atagattcga
    47701 ttatcactat gtacgaattg taaagggagg aacagctaat gcaatatcta gaagcaaaaa
    47761 tcccaattcc agaaggctat gtaattatct cccaagtgga ttatgaggag ttaaaaaaag
    47821 ctgatgatac tggtagatgg atgacgttgc cagaagtgct agaacggatt aacagaaaat
    47881 atgattggtt tacttctaga gttttaaaga acccaagata tagaaatatt atcgatatcg
    47941 agaaaaacaa aaatggattt gtctattatc cagttgaagg aagagacaca tatctatttt
    48001 taagaagtaa aacacttgaa tttttagaaa caaatttttc ggaaatctta aggaggcaag
    48061 cggatgggaa aatttaatag agcgctagtg ttcagcgcac cgctaatcat ctacgcttta
    48121 ggactttggg gaagcaggca agcgttgata ggaacgattg tttacatggt ttggattttt
    48181 atggggcttg atgaagctga agctgagtac agagcgaaaa agccaaccga gggggctgac
    48241 taaaaatgaa tagaaaagag aaactagaat gtatattact attactcagt ttaattcttt
    48301 cactaatttc tctattggga agtttgtatt tttgatatca acaattttgt ggatttgggt
    48361 tctatttaga gtaaatatca tttttgtttg gtgattaagg agtttattaa aattaatttt
    48421 ccctacttca aatgcaaggt aacaagatat agcactttta ggaggaatat ttagcggtac
    48481 agcttcagag tatattctat tatcatcgac tgatcctcct ttagcaatta atttcttaaa
    48541 tggagtagct gaccatctgc ggtttaagtt agcggatgaa agttctaaat taactaacat
    48601 agctggttct gttgaatagt tagaaatgat tacttttgta taaatcatat ctgcagcgaa
    48661 gtaagaagca ttaagttcaa cttctagttg agggcgcatt ccttttattt ttatccctgt
    48721 taaaaacgta ctcaatataa aacttattat tgacatccat tgaaaaattg ttaagtgaaa
    48781 aaaattcaaa ataaacacca ccagttttta actaaattat accaaaaagg agagaagaaa
    48841 taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc ttacaagtgg
    48901 cagaaagctt cgagaaaaaa catcaacatg ttttaagaga tattgatgca ctaaaaaaag
    48961 atgtgtccaa ttttggacag atgtttgtag aaggtaatga accagattca tatggcagaa
    49021 atcgacgagt tttcttcatt agtagagatg gatttttctt gctggctatg gggtttacag
    49081 gaaagaaagc tatctacttc aaacaaaaat acattgaagc attcaacgaa atggaagatg
    49141 ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat gatgatctat caattagaag
    49201 aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga attagcggac
    49261 aacaagagtt tgaaattaag caaaaaggaa atatgaaagt tatgggagtt ctagggggaa
    49321 aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat
    49381 ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg
    49441 ctgtttcatt tattgaaatg tggttgccag aaactgcgat ccgtatggaa attgatcaac
    49501 tgaacagaca acagagactt tttggtgatg acaatgaata gagctgaagc gctaagaata
    49561 gggacggcaa ttgctaattg ctggtggaaa tactataaac caatcatcct aagccaacaa
    49621 catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg gcaagcaaag
    49681 agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa ctttccactc
    49741 tagatcaata tttgattgat cctgattggg gcaagccgaa aattgaggaa acaagtggtc
    49801 gaaaaatcag acgaaatctt ttgacgaatg aagaactagc ttgggatcaa gatgatttag
    49861 gcaaccatgt aactatttgg gatcatgttt atcttatcca tctatcgaag cattcgaata
    49921 aacctgaata tatttacgtc atcgaagatg gcttgattga tgcgctagaa gagtacgaca
    49981 gagataactt gattgatatc tcttattacg gaccaggtaa gaaatacatt gctgaaatgg
    50041 aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt
    50101 aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg ggctcacgcg
    50161 tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtacaaacac
    50221 tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa accttttctt
    50281 atggatgaaa ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca cacggaaacc
    50341 gaattatttc ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa tgcaatgcaa
    50401 atcaataact cattgaagcg atgctttgtg aaagcattgg ctctacacgg actgggatta
    50461 tatgtatttc aaggggaaga tattccaaca ccacctagaa tcgatacaaa gaaattaaac
    50521 atgctagaga cgattctaga agctttcaat gagcagatgg gtaaagatat gaccaaaacc
    50581 ttaatcgaat atgttaatga gcagacagat aaattagggc tcttagctga taacgttgaa
    50641 actattgaac agttaagcta tgagcaatgt gccttgatgg agcgagcaat agcagctaag
    50701 agaaaagaat tagataagaa gtgatatgag tgtttaaacc attaatcgat tcatattcag
    50761 cggttctgaa aaagttcaaa ggaaaagaca taagcgcaac catcaatgag gaagtgaaca
    50821 ttgatcgact aaagacgatg tatgacggct acgatggtga tcgagtcatt gaaattcgtt
    50881 ttattgatcc tagacgtttc accgtacagc aacgaaactt catctatgcg ctgataggcg
    50941 atatttttat cgatacaggc atgccaacgg acttctggaa ggaattcttc tactttcgtt
    51001 ttgaaggtgt cacagggcgc aaaataagcc tgaaagacga atcgaataca actgtgagtg
    51061 atgccaatgt cttagcaaat atcatcttag atttcatctt tgaacatcat attcctttca
    51121 aagaaggcta tgagatttta cctgcgaatc aagaatatta cttctacaaa tgcatcacaa
    51181 aaagagtctg ctgcatctgt ggcaaaacag gagctgacat cgatcacttt gacaaagcgc
    51241 taggaagacg aaagcgcaaa gaagttgatc atgcagagta cacatttgca gcactctgca
    51301 gaatccatca cacagagaag cacaaaatag gtgtgattaa tttcaaaaat aaatatcaaa
    51361 tcaaagggat caaattaaac cagaaaacaa tcaaaaagtt aaatattgga gggtaaaaat
    51421 gacagaacat cgaagttatt atgcgattat accagccaac gtaaggtacg acaaaagact
    51481 taaaccaaat actaagttgt tatacggaga gataacggcc ttgtgtaatg aaagaggctt
    51541 ttgttgggca ggcaatgagt actttgcaga tttatatggt gtgaataaag agaccatatc
    51601 gcgatgggta agtgatttga ttaagtttgg atacttgaat cgggaaatca tttacaaaga
    51661 gggtaccaat caaataatca ataggtacct acgaattaat caatacccta ttgacgaaaa
    51721 acgcaatacc cctattgacg aaaaagtcaa agataataat acatctatta ataatacatt
    51781 taataataca aaagaatata taagagagtt accgccttcg aaaaaatcga aggctaagcc
    51841 cgtccgtcat aaatacggag agtataaaaa tgttcttttg tcagatgagc agatggagaa
    51901 actcaaaaca gaattcccta atgattacca agagcgaatc gaacgactgt cagagtattg
    51961 tgaatcatct ggtaagactt ataaaaacta tttggcaact attcgaagtt gggcaaggaa
    52021 agaaaaaagt gaacctaaga acgcaagcag tggatacaag cgcacaggaa gacgagagaa
    52081 gcttcctgaa tgggcaatcg accaagaagc ctatctcaag aaaaaagcgc tagaacgagc
    52141 taatagacaa tcaaaagcac cattctaaga ggtggaaaaa tgaagatcga ttatctagaa
    52201 ctaattaatg aaatagcgaa ttataaaaag ggcgaggaat tagacgtcct gagagacgta
    52261 tatgatcaac tcgaagaagc tggaattgaa ggaattaaga atgatcgttc gagttggagt
    52321 aagctcagat actatttcgc actttatatc gatacaacac aattaagaaa tttagcatat
    52381 accaaattac tatttgttga ttgtgttaaa ggcttgcaaa aacatcttaa tgaacttgag
    52441 caggtgtaat cagatggacc taaagacatt tacagcacag attgaactaa tgcatcaaga
    52501 agctttaaga caaagtgtat cgtacgaaga caagtggctc aacacgttcc atggcggacg
    52561 tgagagcgca cttgatcaag tactcaaatt actgaaagga gaatgtcggg atggataaga
    52621 aagcggcaat gcagcgaatt atcgaattga cttattcaga agattggcaa aatgacaaag
    52681 aagctgcttc agaagtgatg aggcttggaa gagcgatgtg ggcagacaag agcaacaagc
    52741 caagaccacg aaaaatcgca atttggcacg gtgacaaact tctagtgata gggacagctg
    52801 aacagttagc aagtctcaca ggcttgcacg agaaaatcgt gaggaaaaga gcaaggtgtg
    52861 gctacacaga cgctaagaag agaacgttta gatacgtgga ggaatcatca tgacaacaga
    52921 agaagtgatt caaatgcgta ttcgaagcct tcagcgtgag attgacgatc tggaacgaac
    52981 aaaggcagtg atggtcaatg aaacggcgag aaaggcaatc gatttgcaca tagagaattt
    53041 aagaagggaa atccatcgat tggaggaatg agcgtggata agaaagcagc aatgaaaaga
    53101 attgctgaat taaccaagtc agaatcttgg caagaagaca aagaaatagt tgcagaagtc
    53161 caaaagctcg gtaaatcaat gtggactgaa aagcccaaac ggagaacgcc gagaaaaatt
    53221 gcaatctggc atgatgaccg aattttagta acaggtactg ctgaacagtt atctgaaatt
    53281 actggattaa gcaaaaacat tatctgggat agagctagga gcttatggat tgattcaaaa
    53341 ggacgacagt ttaggtatgt ggaggagaga taatggatct cattacacaa tacagtgaca
    53401 tcatcctcaa gaaaatcatg atgaagattc agaaagataa aaaatcaaaa gaacgagctg
    53461 aattagttaa gttagaaatg gctgaaacag gagcaggagt gcgaagtagc aggcattgga
    53521 aagcagcagc aaacattgaa ttttattaca acgaaattca aaaagggttc gatcagatgc
    53581 gtgagctgga tcggcaaaca aattggagca agaaacttca tcaagatcgt ttcaaatttg
    53641 tagagaagta taaagaaata ttagaagagt atttgaggag gacagcaaat gataaaaaaa
    53701 ctcgttcaat tcagcatgga tttatatgat atcgaatcag gagcaacact atctgtggaa
    53761 tcggaccatc taatcataaa ttttggtgga aagcgccaga ttattttgtg ggtagttgat
    53821 gatgtactgt ttccagaaat tgttcatgat ttcgaagaat caaaagcggt tgagtttgaa
    53881 atagtgaaaa aagtaatgga attgattgaa aaatacgagg aggacagcga atgataccga
    53941 agtttagagc gtgggataaa cgaaagaacg taatgagaga tgtagccgtc ttgcatttta
    54001 ctaaaaacgg caaaacaaac tttattgaat attggataaa tcctaccgaa ttgaaatcat
    54061 atcacgtgcg aaacatcgac ctcatgcaat ccacaggaat gaaagataag aatggtgtgg
    54121 atatatttga aggggatata gtattagtca gcgtgcgaaa tggcttcgat tacttagata
    54181 ataaagtctg tattgtcaaa aattcaatag attattccgg attagtttgt gccactgttg
    54241 atgaagactt agagtatcaa atttttaaca cagagctgtt tgaagaatac acgtacgaag
    54301 tcaccggaaa tatatacgag aatagcgagt tattggagga acagcgatga ataaacagga
    54361 agcaatcaaa aagttagaaa gtattaaagc gataggaaat gatgcaatag ctgcttgcta
    54421 taacgagagt ataaattcag gtattacgtt aatgaaaaaa atagacgaac cgcagaaacc
    54481 agttattcca caactcgtgg ccggttggct tgagaaatct acggaccctt ttacaaaagc
    54541 tgaaaaaata gcgtatttaa tcaaatctaa agatggtgat tcatattatt tctgtgattg
    54601 gtttgtacga gatggcatag tgacgcaaga gcaaggagaa gaattactcg cttgggcaac
    54661 aagacaatca tatgaaacac tattgagcct atacaacggc tacgaggttg agaaagagcc
    54721 gttatgggca ataaagaatg ccgatggaaa ctatcttact aaatgtgctt tatggggaaa
    54781 agatggagta aattatagtt ttgaatgcaa tccatctcat cgattgcttt tcactgataa
    54841 agcaacagcg gatgctgcag cattgttggt gaatggaaca gtggaagagg tggtagaaag
    54901 atgaaactaa aagacggatt ttacgctagc agtcacggta tcggcggttt aatgctagat
    54961 atgccgacaa agaaccctaa aacacgcaag aaatcaaaat tcaaagtcgg tgacatggtc
    55021 cgctgtgaag cagaagagtt cgtttatccg ttcagaggat acgttaaaaa gatactgtca
    55081 aactcagcaa tcattcgtat tgaaaataca atgaagtgtg atcgttcgac tgcaaaacac
    55141 aagcattatt tagctgtagc gagattagtt gacatggaag taatcaagag caaataaaaa
    55201 aagccggatc gctccgactg attcaataaa tccaacacat ttattatatc acataaagga
    55261 gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa gatgtaatgc
    55321 gagagatgtg ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct ctttgataga
    55381 tattaagtcg ccgattataa cggatatgcc gaaggcaccg aagcatggca ataaggcaga
    55441 agacgcgatt attcagatga tggatataga aacggagaga gatgcgattc tagcggcttt
    55501 gatggctctt agtctgatta gtcgtcagat actctactac agcttctgtg tgccagatag
    55561 cttctcaaac tacagaatta gccgtgaagt gggttattca gaaagaagta tacaacggat
    55621 gaagtcggaa gctctaatag agtttgcaga agcatataaa cacggaaaaa taattgctta
    55681 taaataattt ggcggttttt tggcggaatg atggcggttt ttagacattt accagtgtta
    55741 ttatggtagt gtcgaaagat taggaaacag gatcgacaaa ataaacgaag ggaggcaatc
    55801 tccctcatcg ttttaaactt cttcttagat agacagcaac taattatgaa gaaaaggatg
    55861 tgaattcaac tcctcctaaa ttcttcatcg gttgctgttt attttgatta gacagcagca
    55921 ctcacaaaac taaacatgtt cttgtcgtct ttcaactgct gctgtctatt aatttatgta
    55981 ttggagggaa gaaaaatgaa attatcagta gaaggcactc cagaagaaat acaagaattg
    56041 ctccaagcta ttggtagtag caaggagcaa aaggtaagtg tttctaattc agatattgac
    56101 ggtatttgca attgtttaaa cagagaaaaa atatctaatc cttactaaga gaaaatatgt
    56161 tcgttaatga atttccaatt ttcctctgta gctctacctt gataattttt ttgcagttca
    56221 cagatgaata aggaatcgtt attatcaagt actgtattta atttttcaca catttgattt
    56281 ggattcaaat tagttttaac taaccaaaaa gatttttgta gctttatata tgccccaaat
    56341 tcttttatta tttcaaatac tttatcatac ttttgccctg ggttattaag atcataacta
    56401 atcatgtagg gtttatacat gttgtcttgc acatccttgt tttatatttc agcggaccac
    56461 tcgctgataa ataaaattat acgcttagta tttattttca caatattaat ttatcgtaat
    56521 aacttttgag taaatagaac aaaaaacctg cactagtttc cgctagtaca ggcagtgact
    56581 atatcggttg ataatctagc atatattaaa aataattgca agaaaagaaa tttatagtgg
    56641 tttataccaa attatcaaag caatgttatt ttgttgctgt ctattgtttt ttaattattc
    56701 acacgataac taaaggtggg tgaagagaaa tgattccatt aataatttca atttttgcgc
    56761 tctgtcttaa tgtctatatg attggattta aaaatgggca aaataaaaaa tagtagcagc
    56821 caagaataat tttatagtgt cactgtggcg gaaagggtag acgctaagca tgtgtgctag
    56881 gtcaatgctt cggcaaccat gcaaggttcg attccttgcc agcgacatta aatgcctatg
    56941 acggttacga ctaccgaaaa aagatcgtta agaagctata cggtgctacg tacggcaatg
    57001 tagtaagtgt gctatctgta caccaccaag cttcggtcac tgtggcggaa gtagaagacg
    57061 cagcggtaaa tggcgagtag cctcgtgaga gcctggtaag ttctcgtgag tggtgcaatc
    57121 ccactccagc gactttaagc aaccgaggca tcggcggttt aaaaatatag gggtgcgcaa
    57181 tttcgtacgc gttttgtgca tcgtgcaagt tactattaca tattagatca ctctttgagt
    57241 ggtcttttta tttttgcaca aaggaggaaa caacaatgta tagaccacaa tacttagaac
    57301 agaagtatga agtaatcact gttcataaag gtaatggcga aaaagtatat gagtatagaa
    57361 gaccaataaa gagcgataca tataaacgaa aggaaaacaa tgaagttatt ccattgtatg
    57421 gcaaaagaat agctaagcat taaataagat tgcgaaagga gacggaacat gaccgaggaa
    57481 ttctatagat ggctattaca gttgacaaga gaagatcgtt tggttaagtt ctatcagtct
    57541 cctaaatggc gcaggcttag agagaaagcg atgaaacgag atcactatga atgccaagag
    57601 tgtagaagac taggtaagta tcatagagta gagaacgttc atcatataaa ggaagtcaag
    57661 gatagacctg acttagcttt agatttagat aatcttattt gtttatgtgt tgaacatcat
    57721 aatgaagttc atggcagata tcttacagca ttagataaac aagagaagaa gatagaaagc
    57781 tttgctaact tcgatgcaag tgaaaggtgg taagtgcatg atcatcaatg ataatggcag
    57841 agagtatgat acagaaaaga ttgaagagta ttcatcttat actcagggat taattaaacg
    57901 tttgatatac gttcgctatg taggtattag ggatctgtta tcagataact gttgtagtaa
    57961 atacaaagtg aatcaagtaa gagaagcgtt gaataaagat aataacgtcg aaagaataaa
    58021 aaatgttttt ggatatagta ttgaagagat taattattac attgacttcg ctgaagcttt
    58081 cattccgatg gtgagataac ccccccttaa aataaatcgc aaattttttg ggggtgatga
    58141 aacggagggg gctgtcagga aaagagattt tttcgaactt tatcatgaaa ggagggctaa
    58201 aatgtttaaa aacgaattgt ctcaaaatcg ctacagagaa aaattacgcc gctctttaat
    58261 aagccaattg gaaagtcaga aaacaaatat tgagccattc ttagataatg ttgatcgtta
    58321 tatcagttta tgggaaacgg cgatatcact ggaagaagat atatccgaga acggcattag
    58381 actggagaat ggtaaaaaga atgaatcagt agcgttgctt gtttctgtca acaaacaaat
    58441 gggattgatg ttggataaac ttgccattac tcctgaattg gtaggtgaag caaatgaatc
    58501 aattcctgag ttataagcat attgaaaatt ggttcaaagc tatagaagaa ggcactatca
    58561 aggtatgcaa agagcaatta ttgctaaaaa attatctaga agaaagagtc tttactagag
    58621 aagatattta cttcgataag cagatggtag aggattcaat caatatacca gcacaatact
    58681 ttccattcga attaattccg tgggaaaaat ttctacaatg ttttatttat ggtgttcgat
    58741 ggaaaaaaga taaaacacta gtgttcaata gatatctttc attaatggga cgtggtaatg
    58801 gtaaaactgg ttttgcttct tggaacaact tctttctact aaccgctaaa cacggtatta
    58861 aaaattatga tattgatatc tatgccaata atgaaagcca agcaaagact agttttgatg
    58921 atgtatttaa agtaattaaa gatcatcctg atttagataa aaaagtattt aaagctacga
    58981 aggaagttat tcaaaatatc gctacaaaca gcaaacttcg ttataacacg gcaaatgcta
    59041 gaacaaaaga tgggaagcga ccaggtgcaa accgctttga tgaaattcac gaaaatgaag
    59101 attattcaat gataaatgtg gctacttctg gtggtggtaa aattcgagat tatagagaat
    59161 tttatgatac aactaatggt catgttcgtg gtggtccgct tgatgacatt atagaagaat
    59221 caaaaatgat tctttctgga gaacttggaa ttgacaagga tggagcagaa ttttctagtt
    59281 tgtttccatt tatttgtcgc ttggataacg ataatgaagt tgatgatccc gacatgtggg
    59341 aaaaagcttg tccaactatt aattacaatg cagatttaaa acggaaaatg tttcaagaat
    59401 actctcaaat gcaacgtaat gctggtttaa gacttacgtt catgaccaaa cgaatgaaca
    59461 gacctatgga agatacacga tttgctgttg cttcatatga tgatgttctg catacgaaag
    59521 aaaaagaatt tcctgaaaaa atggatgaag tgataggaac agtcgatttt gctgatagac
    59581 gagattttgc cagcgttggg ttgctaggaa aatacgataa agatgtttat tttacacaac
    59641 atacttttat ccacgaatca gcccttcgat tacaaaacat caaacgagag gttatagata
    59701 tttctataga tcaaggaaaa tcacagatcg ttcatggaaa aaatatagaa gctgattata
    59761 ttgtaggttg gtttcttgaa atgagtaata aatattatat taaaaaaatc gctatggata
    59821 tgtaccgtgc aaaaatattg aagcccgctt tagaagaagc aggttttact gtggaaattg
    59881 ttcgaagcgg atctgttaca catggtatgt taaaagatct ggttgatgac ctttttatta
    59941 atcaacgttt attttttggt gacgatgcga ttatgcgttg gtattgcatg aatgtatatg
    60001 aagagcatat ttctaatgga aatatacgct atgaaaaaat agaacctgaa actagaaaaa
    60061 cggatggctt tttttcattc cttcatggtt tgaatttttt agatgatatt tatgattctg
    60121 ctcctgtaac agtcacaaat agctcagtag aaaatacagg aactggattt actcctctag
    60181 tattctaact tgaaaggagg tgagaaagtg gggatttttc aaaaggcggt aggatacttc
    60241 acaaaaaaag caacggttcc tttagaagaa tacttttgta aattgcaagt tgattttgtg
    60301 tatcgaaaat ttgcgattga aacttgtatt gatttgattg caaatgcgat gagtaaagcg
    60361 gaattcaagt catatgaaga tggaaaaaat aaaaagaatg atctttacta taggctgaat
    60421 gtagctccta ataagaaaaa taatgcaaca gaatttagaa aaaaactgat caggagatta
    60481 atattctaca atgaagtatt gatcgtttct ccgtctaata attctagcga aatatttatt
    60541 gcggatagtt gggatgtcac agaatatgca ttgaaagatg atgtgttttc tcaagtgcaa
    60601 attaacaaca tagtccttga tagagaattt ctagaaagtg atgttatcta tataaaatac
    60661 gcagatcaac aaattaggca actagtcgat gcgtattatc aagcgtatgg gaaactcatt
    60721 tctagtgcca tgaatgttta caagcgctct aacgctcgta gatacgtact gaaagggaat
    60781 ttattccgac cgcaagacaa tacaacacaa gatcaaatca ataaaatgat gacatcacaa
    60841 tttaaggctt ttatggaagc tgataatgca ggtgcggtat ttcaattaca aaatgagtac
    60901 acattagaag atttcagcgg aaacttccaa agcaattcaa gagatataaa aaacttaata
    60961 gacgacatct ttgagatgac agcagcagcg tttcacgttc cgaaaaacct actaaaggga
    61021 gacatgagtg ggttatcgga tcaagtggac gcttttttaa tgttcgaaat cataccgatt
    61081 gctgaactta ttcaggatgc gtttaacgct agtctctatg aagtagaaga atacttgtca
    61141 gggaattttg tacgtgtgga tacaactatg atcaagatta ctagcttcaa agatttggtt
    61201 gacgctattg atgtaggcat tagaaatgga gtatttacaa tcaacgaagg aagagaacgc
    61261 gttggaaatg atcgctctga taaggcgatg gcagatgaaa tatttataac taaaaacaat
    61321 caacaagtat cgaaaggagg tgaggcgaat gacgacaatg aaaacatttc tagcagtaaa
    61381 gaatgaaggc gcagtaccgc aaatttttat tcagggattt attggttcta gttggttctt
    61441 tgaagggaat actgacaagg gaatcaaaaa tattttggat agtctaggtg atcaagaaga
    61501 aattgaagta gtaattaatt caaacggtgg agacgtattt caagggattg ctattgggaa
    61561 cttacttaag tcaaataaag caaaagttaa cgttgtgatt aacggcttag ccgctagtgc
    61621 tgcttcaatt atcgcaatgg ctggcgatac tataaaaatc tacaacaatg cacaattgat
    61681 gattcaccgc gcttccacat acggagaagg aaatgtcgat gacttccgta cgattgctga
    61741 ccaactggaa tcaattgata aatcggtaaa ggcttcatat aaaacacgat tcaatggcac
    61801 agatgaagca ttgcaagaac ttcttgaaaa agaatcgttt atggatgcag aaacagcttt
    61861 gagttatgga ttggtcgatg aaattatcga tgcagaaaat agctcaggta ctgaagctaa
    61921 aaaagaacaa agcgttgaag aaattttgaa tgacgttaaa gaaaaaagag cagaaaaaat
    61981 tgctgcattt acagcagcat taaataaaac atttggacaa ggagatgtaa aataatgaca
    62041 gttaaaaatt taaaaggtgt aacagctgca agcgaccaat tgatgaaagc ttttaaagat
    62101 ggtaacgaag aatcttttag cgcagctatg gtaagcttat ctaaggaaat tcaggataaa
    62161 attttagaag aagcaacagc aaaaaatcaa gatcaattag tattaatgaa ccgtggtcag
    62221 cgtgtattaa ctacacaaga aacaaaattc tataacgaag tagtgaaaaa cgaaggtttt
    62281 gcaggggtcg aagaattagt gccagctact gtatttgaac gtgtatttga agatttagaa
    62341 caatctcatc cactattgca aaaaattact tttgttaaca caactggtgt aacagaatgg
    62401 attgtgtcac gtggagtcaa tccagcatgg tggggtaaac tttgcgaagc tgttaaaaaa
    62461 gttttagata atggctttga tgtaattaac atgaagcagt tcaagctatc aggttatatt
    62521 cctgtatgta aggcaatgct tgatttaggt ccagtatggt tagatcgtta tgtccgtact
    62581 gttttagtag aatcattgag aattgcatta gaacaagcaa ttgttgatgg tactggtaaa
    62641 gatatgccag tcggaatgat gcgtgacatg agcaaacaaa ctagcggaga atatgctgaa
    62701 aaaaaagcag aacctattac agctttagat gctgtaacta tgggcggttt gatggcgcga
    62761 ctatcaaaat tcaatatcga aggtgtgaat gatccgattt atcgtaatgt gaatccttct
    62821 gatgtggtcc taattgtgaa tccaactgat tactggtcta aagtattccc agctaagact
    62881 gtactaactg ctaatggaga atacgtacaa gtattgccag taccagtttc agatttgcag
    62941 tcaacggctg tgccagaagg aaaagcagtt attggggtag cctcagatta cttcatgggt
    63001 gtaggatcta cactaaaaat tgaagcttca gatgaatacc attttgttga agacgaacgc
    63061 atttatctag ctaaacaata tgcaaacggt caacctaaac gtaacgatag tttcattgta
    63121 ttagatatta gcgctttggg aactactact acaactacaa aaccaacaac cacaacaact
    63181 acaacacaag cgtaggtgat cagaatgaag tatattcttt gtcagccggc aatcaatcgg
    63241 tttaaatggg agcttgaagt ttgtttaact aatctgaaga aactaggaat caaagatatc
    63301 gtattgcttt tcagcagaca cgatgatcag attcctattt tttttgagaa ggaatatggt
    63361 gttgaagttc atgtgtacga tgatctgcgg gacgacaaag agtatattcc ttcgattaaa
    63421 ccatatttat ggtggaaata tttagaagaa gatcattcgc gtgaggacga ccgatatttc
    63481 tatatcgatt cggatgtcat tttcaataaa agaattaatt tgcgcaaatt gccttctaaa
    63541 gatgatgttt ggtattgtag cgactgctgt agttatctaa gtcttgatta tattagaagc
    63601 tgtgaaaacg gagaaaatat tctaaaagat atggcaaaca ttgtaaatgt tacagtagaa
    63661 tctttggaaa ctataaacac taattcagga ggcgcacagt gggttattaa ccgtcctaaa
    63721 gcgaattatt ggaaaaaggt ttatctggat tctaatcggc tatatcgcta ccttagaggg
    63781 caaaaaacaa atatacaaat ctggacagcc gagatgtggg cacagctttg gaacatgatg
    63841 tatttcaata ttggtcctaa agttcacgag gaattagact tttgttttgc tactgatcca
    63901 atagaaaaag ttaaagaagt aaaaatctta cacaatgctg gagtaacaac aaacgatgaa
    63961 gatttatttt tcaaagggag atacgtgact tctacgcctt ttgatgaaga tttatcattt
    64021 gtaaacaaga aaaaatgctc ttacgcatat gttaaagcaa ttaaggcggt ggttagatga
    64081 cgcctgaaca agtgactgaa gaattgctaa tagctgtgaa ggataatatt tacgttacct
    64141 ggaacgaaga agatgagtca attaaaaaga tgatagctaa aaatgctgtt tatcttcaaa
    64201 gtaaagtgag tacaacactt tctttttctc ctgaaagctt agaatacgga ttgctaatcg
    64261 aaagatgtag atacgactgg aatcgtgctt tagatgagtt tgaacaaaat ttcgctagtg
    64321 agttattagg tttcattcaa cattatgcgc tacaagaata tattgcaggt gatgtgaatg
    64381 gcgaataatc gtagactcga agaaacattc aacgatggtt ggttaaagat tttgacgcaa
    64441 actaccaaaa gaaatgaact aggaaaaaag attggtgtag aagatacaga aatcacttct
    64501 ttaaaattta gaaatctttc catgagagat agtgatataa cagctatgga tgcgatggga
    64561 tcgaaattaa ctaagaaagt aaagactcca tttcatccaa tcgccaagaa atttaataaa
    64621 gatcaatatt ttatcgtaat cgatagtatg cgttacaacg ttatctatgc cgattacgat
    64681 aatttttata tctattttta tcttgaaagt gtgggtgaat atggtgattg ataattctaa
    64741 agaaaaagaa cgtttaaata agcaaatttc tgctatcaaa acttccttag aagagcattt
    64801 tggcctcaaa ctctttcaag actccgtagg cgaggatgag ctacctgatg attttaatta
    64861 cttcattctc gaaacaggag aaatagaaat gatcactgag ccaaaatata gcgtgggtca
    64921 aaatctatat ctaactttct attcagaaaa tagagaagat ttaacaggag actcactaga
    64981 tattatttca ttgattcaaa atcgttcgat tcgttttcag agaatggatc ccaatcattt
    65041 aaaactagag aaccaagatc gctatatcga tcaattggta tttacgttta gacgattatt
    65101 gaagagtgat tgtcatggct aaaaatagtt gggagctaaa aataaatgga catgatgaac
    65161 ttcttgtgcg gatggaacgc tattcaagcg agagcgaacg actgattaac gaagcattga
    65221 aatcaaaagg ttcggctatt gcagtggata ggattacaga aaaaattcct gtttctgaag
    65281 cagatttaag aagagggcac caacacgcaa aaaatagtcg tccacttaag actcaataca
    65341 ttaatttggg tttcatcatt agacctacaa gaaaatttga gtatttaaaa tatcctgatt
    65401 tggggatagg tacttctaaa agaaatcagc cggacgaatt tatgagaaga ggattaggtc
    65461 ttgcacttga tccaattaca gaacttctga ttcgtcaatt cgataaatta aataaatagg
    65521 gggaacaaca atggctaaaa caaaaactgt agtaacaacg ttcgataacg taagtatcaa
    65581 acgaattgct tttaatttta agaacgcagg aaatgcaatc gcaacgaatt gtaatggaca
    65641 attagatggc gaaacagaaa tgcaaacggt ggttaaaaaa tgtggagcga cagaagtaaa
    65701 atcaaaatct aaaccaatca atatgacggt aacaattact gcacatgtac cgatggaagt
    65761 ttatcgacgt ttcaatgggt tgaaacaaga tggacgtatt aaaccaggca tttactctta
    65821 cggtcctgat tccgtaggcg aagatttctc acttgctgca gagatcgtgg atgacttcga
    65881 agaaaataga aagttagttg gtatgttagc atgcacttcg aatacaggat taacattctc
    65941 tattgaaaat ggtgcggatg aagtagctgc gttagaacta gaaacaaaag ttatgcaaga
    66001 tgaatttggt aaattttatc atgaagcaat tgttgcagaa cttgaagaag acttaacaga
    66061 tcaatggatg acgaatctat ctgctgatgt gattaaaaaa acttcagttg tgacaactac
    66121 ggccactcaa tcacagtaaa aaaaacggag gtagcgaaat gaacgaagat tactcaaaaa
    66181 ttgaactaaa cgatggaaca attttgaatt tagaacctaa actgaatatc aagaaattat
    66241 tgatgatcaa tagagatttt aacacagacg agtttgcaaa aatgactgtg ggaaaaggat
    66301 ccatggatat ttctgttatt caaggtgcaa aggctgtgta tattgcttac cgccaagcga
    66361 acatgactga ttatatttca ttcgatgaat ttatcgataa atgggatttt gatatggcta
    66421 ctgccagcta tatttatcaa ttgatgatgt tcaaacaagc acgcgatgct tatcaaaaag
    66481 aatttgaaaa agcaaataag gaaaaaaagc ttcaaaagta aaaattccaa agctcttagt
    66541 tgaaacgtgg gtcgatgtct attcgatgtt gaccgacgtt ttttctatgc cttcagattt
    66601 ggttttaagc gatatctgtt tagatgacat tttgcaaatg gcttacaaca agagtgctta
    66661 tgaaggatgg aaaaactatg caataaacca atcccagaaa aactaaagaa aggaggtaaa
    66721 aaatggctaa aaagagaaca gaagcagaag taactttcat agctaacgat gacggattga
    66781 aatctacgtt aaaagaaatc agtgctgaat taactaaaaa tagagcagaa ttaaaactag
    66841 aacaagctca attacaacag actggttctg aatcagacaa gttaggaagt aaattatctt
    66901 ctttagaaaa gcagtatgaa ttacaaagtc aaaaagttga agtaactagc caacgtttag
    66961 ccaatgcgaa aaaatattat ggagaaaatt ccaccgaagt tcagaaactt gaaagagaac
    67021 tgattaatca acaaacagcg caacaacgtt tgtcaaacga aattgataaa acgagtaatg
    67081 cactagctca agcaaaaggc gaaatacaga cgtacgagtc tacaatgcaa cagttggata
    67141 gcgaacaaaa aaatgttcaa gctagtgctt ctctgattga atccgaatac aaaaaatggc
    67201 aagcaactgc tggtcaatca gcttctgaag ccgagaaatt agcgaaagcc caagaatatg
    67261 tttctcaaca atctgaaaat gcggagaaaa cgatagatat cctaagacga cagttagaag
    67321 ctacacagtc tgaatttggc gctacatcca cagaagcaat gcagatggaa gcgaagctta
    67381 atgatgctga acgtgaattt gaagagttag gacaagctgc taaaaatgta gatacaacta
    67441 acttggacga tatcggaagc aaaatagata tgaataattt aatggaagct tctgacgttt
    67501 taagcgacat tggcgataag cttacagaat tagggaaaca agcagtggac tctgctaaca
    67561 gtgtaggtag ttcccagagt aagatacaag ctaattttgg tttgtctaaa caagaggctg
    67621 aagaattaac gaatgtagcc agagacattt attataaagg ttttggagaa tcgttagatc
    67681 agtccacaga tgcattgatt ttggtaaagc gtaatttagg agatttaaat aatcaagatt
    67741 tacaaaatat cacggaacaa gctatggtcc tagaaaacac catgggcgct gatatggatg
    67801 aaacgttacg tggtgtaaat ggcttaatgg tcaatttcgg cttgagtgct caagatgcaa
    67861 tggatttaat ggtttcgggt actcaaaacg gtttagataa aacgcacgaa ttaggcgaca
    67921 atatggcaga atatagccaa ttatggagtc aaatgggata ttcagctgat gaaacgttcg
    67981 gaatgcttca aaatggttta gatgcgggtg cttataacct tgataaagtc aatgacttag
    68041 ttaaggaaat gggaatatcg ttaacagatg gtcgatttga gcaaaacatg gatatgttta
    68101 gtgaaagtac tagaaaagct tttgaagagt ggaaaaatgg cggaggaaca caaaaagacg
    68161 ttattaattc catgattcaa gattttagca atatggatgg tcaatacgac caattaaata
    68221 aagcttcgac aatttggtct gcgcttggcg aagataatgc gatgaaagtt gtccaatctt
    68281 taactgatgt taaccataca tttgatgatg ttagtggatc tgcacaaaaa atgaatgaag
    68341 attctactac tccgttgcaa gagttgaacg ggaaaatagc tgaattaaag gattcattag
    68401 ctcctatagg caacacaatc atagatgcac tcgaaccagt aattgatttt ctaggaaaga
    68461 tggctgatgc gtttaataat cttccacaac cagtacagga tttcattgtt gctataggtg
    68521 gtcttacagc agcatttggc atactgttac cggcaatact agccgtgtca tatttatttg
    68581 gtccgatgat gcttataatt ggaggaatta tagctgttat agcaggcgtt attgtggcaa
    68641 ttaagaactg gggtgcaatt actgactggt ttagtggttt atggaaaaaa tttactgatt
    68701 ggttgggtga tacttgggaa agtataaaag acggagcctc atcagtttgg gatggggtta
    68761 aagaaacctg gtctggattt gtagattggg ttcaagatat ttggcaagga gtttctgatt
    68821 ggtttggaga gctatggagc ggattagttg aaggagcttc caacatctgg caaggagtcc
    68881 aagaaacttg gcaaacattc gttgattggg tttcaaatat ttggaacgga gtcaaagaag
    68941 tatggtcgat tatttgggca gacattgtag gaattgttca aataccatgg acattaataa
    69001 cgtcattgat tcaagctggt attaatatta tcgtgggtat ttttgatgta gctggacagt
    69061 tattgggcgc agcttggcaa gctgtttgga cacctatttc tgatttcctt aaaaatactt
    69121 gggatactat gacacaatgg ataagtatcg cttggaatgg gattgtaact acattccata
    69181 ctatatttga tccagtagtg gcatggtgga atggtatatg gacagctatt agtactacgg
    69241 cttcaaatat ttggaattca attagtgcaa cagcttctag tatttggaac agtatcaaga
    69301 atacaatcac tagcttggta caagcagctg ctacagtaat tcaaaatatt tggtcaactg
    69361 tatctagttg gttaggtgga atttggaatt caatcagctc tacagcatca aatatctgga
    69421 atagtgtgac tagtagtata agcaatgcta taaacgcagc taaaagtgcc attcaaagtg
    69481 tttggaatag tatatcttcg tggattagcg gaatttggaa cggtatcaaa aatactgctt
    69541 tgaatctttg gaatggaatt acaagcacta ttagctctaa agtaaacgat ggaaaaaatg
    69601 caatttcaag cggttggtcc aatctaacag gtattgtttc cgacatattc aataatgtta
    69661 aaagtacaat tgctaacata tgggaaggca tcaaaaagac tgttagcgct ccaattgatt
    69721 ggattagaga caaaatcagt ggcatttttg ataatttgaa tatttctata ccacatattc
    69781 cgttaccgga atttatcatg gaaggcagct ttaacccgct aaaaggtcaa atcccccatt
    69841 tgcgtgtaaa atggcatgct aaaggaggta tctttactaa accaacttta ctaggtggaa
    69901 tgaacggtgt cggtgaagca ggacctgaag cagttttacc tttgaaaaga tctgttttgc
    69961 aagaaattgg tgatcgtatc ttgagtagca catcagtttc atctagggca caaacgattc
    70021 aacctgtgaa taactacgaa ttcaatttca caattgatgg taacgcagat gaggttacta
    70081 tgaagcaaac aactcaacaa atcattgata gcattacaaa aattcaaaat gataatgctt
    70141 cggcatggcg ttaaacagga gagtatttct cctgtttttt tagtattaaa aaggatgtga
    70201 aaaaatgact gattgtatac attctataat cgatggattt cctgattatt tgcataaatt
    70261 ggctttagct gaaagaccaa ccataccttc tccaaaaaga cagagagttg aaacttctgt
    70321 tttaggtagg ttaggtggct tagtacaaga ttactcgttt gaagatatgt cgtttacatt
    70381 gcactataac tatttagagg atgtggaaga ccatcaagcg ttcaagcaat cgttttatat
    70441 catgcgtcat tggttaaact atgcaaagaa attagaattc tctgacgatc ccaacgtcta
    70501 ttacgttatc cagactatcg atattgggga tgcagaaaac gatattgttg aatggggaga
    70561 gttcgatgta aatattactg cgaaaccatt cgcaagagtt caagaagatg tacctataac
    70621 cgtagataaa ccacagtcat ttaacttgct gaataatagt ttagaagaaa gttttccaaa
    70681 gattatcatc actccttcag ctacttcatg ccagttcatc ttaaatgatt atgtgtttag
    70741 tttcgaaggc ttagtagcag gaactgatgt agtcattgat agtgatttga tgctttgcta
    70801 cgaagagcaa tcggacggag atattttaga tcggtccaac aaaatgaaga ccatgcaata
    70861 tccgacattg caagtggata tcaatcattt taattgcact ggtttgagca aaatacaaat
    70921 ttatcgtaac gggttaaggt aggtgaaata gatgatcgat aatttaataa ctatttacga
    70981 taaaaatgac gcgaataatt tagctgaaca tttatatgat acgcaaggtt taggcgcttt
    71041 gtcagactgg ttaacagcta ctgttagcaa taaactaaac ggagccgaga tatttcaggg
    71101 tacttattca ataaacggaa ctaatgcaga tttgattgta gacggacgta ttattcagtg
    71161 ttatgtagat gaaaatcgag caaaacagcg tctacggatt tattatgcaa aaacttctgt
    71221 aataggcaat acgatagaag taaaagctga acctattttc aatgatataa gaaaatcggt
    71281 gttgaataaa tatgacagcg gaacagaaaa gatcactgct actcaggcat ggcaaaacgc
    71341 aaaagtttta gcgaaaccag ctatcccttc gcagttttct ttctcgtcat tagtagatac
    71401 gcttgctaat gtgaagatag aaaaggcgaa ttttttagaa ttctttggtg gaaaagaggg
    71461 atctattcta gatcgatttc atggtgagtt tctaaaagat aataacacat tacgtcatga
    71521 aaaaagtcta ggtacggatc ataaaatcaa agcgatttat actaaaaact taactggtct
    71581 tgacttagag attgatgctc aaagtgtttt agttggagtt tatccattca ttagcagctc
    71641 ttcagaagga gaagacgaga tcactctacc agaagaagtt attttcacgg attacgtgga
    71701 tgattatcct gctggatatg tttcttttgt tgattttaaa gacaaagcga ctgatgtagc
    71761 cacattaagg gaagctgcta aagactggtt gaaaacaaac atagataaac aaaaaccaca
    71821 agtgagtggt tcgattgaat tagtaccatt gaggcatcaa agaggctatg aaaaatttgt
    71881 tgatctagaa aaagtttcga tgggtgacgg agtagatgtg tatcatccac agttaaaagt
    71941 gaatatgtca gcgagaattg tggaatatac gtttaatgtt ctaaccaatt catacgataa
    72001 attagttgta ggaaacgtca aaacaaactt cttagaaaac acagagaata atgtcagcaa
    72061 tttgattaat gatgccattg atcaattgaa aaacggtggc gaaatcagtg atttactcaa
    72121 tgatattgta gatcatcaaa ctgatatgat tactggtcaa aatggtggtt atgttttatt
    72181 agatcctaaa gaagcgccta gtcgtatttt gattatggac acaccagata agaataccgc
    72241 aaggaatgtt ttacaaatca acaatgctgg tattggtttt tctaaaactg gcattaatgg
    72301 aacatatgaa acggcatgga cgttagatgg cggattcaat gcctcgttta ttacggctgg
    72361 tgaaatagta ggaattacta ttagaggtac tacattaatt agtgatggtg ctgattatag
    72421 aacaagtatt gctaatggca aaatgacttg gtactcaaaa aaagttaaca aagatattat
    72481 ggagctagaa gcacgtgatt atgtaagtgc tgatgccggt attgtatcat acaccatgaa
    72541 aactggtggt ggtttcatga ttagaaatcc acagggtaac ttggttttta gtacgtggga
    72601 taatggtaat aacagaccgt ttctatcttt tggtgcgccc aatttcaggt atagcaatgc
    72661 tagttatgta acttctggcg acggtagttc tttaagcatt aatggtagtg cgggtaactc
    72721 atgggaattt aaggtagctg gtaggactat gaaatttact agtgatggta tgctaacgtt
    72781 accaggttgt ttttttggtt catgggaaga tgggaaactt gctaggtttg aacaatcaac
    72841 ggtacaagta tataaagatt ttactgttag aggtactaaa aactcaactg taccaacaga
    72901 acattatgga caacgactat tgaacgctta tgaaactcca gaatattatt tcgctgatta
    72961 tggggaagcc gttacaggtg acaatggtaa agttcgtgtt gatattgacc ccatgtttgc
    73021 tgaaacagta aatctaagtc ggtatatgac acatgtgaca cctacagaac tagttttgtg
    73081 tgctgttact catgaagata ttgaccattt catcattgaa actagtaagc caaacgtatt
    73141 agttagatgg aatttagtgg cacaccgtct agggtatgaa gatattagat taaaagagga
    73201 tacagcatat gatagcacag tgcttgacca aaaacgtttt taaaacgaag acaaggaggt
    73261 atataaatgg ctagcagttt atataatttg gctttagatt tcagcaaaga attaaactac
    73321 accaaagcta ttatggctcg tcaaggtgat aaagggatta cggtgacggt taaaccgttt
    73381 ctaaatggct tgcagatgga tacgagtggc ggaacattta ctttaaaagg aacaacacca
    73441 tctaaccgtt acgtagataa tgttgcaact agtgtaacta gtgaagaagt cacgttttct
    73501 cttgatggca catttatgag tgaagcagga tattataaac actgctacgt agaatataga
    73561 aaagacaatc aaattttaac aacgcaagat atcatttttt tctcactagg agtgtctgac
    73621 atttcgcaag gccaagccga tgaatatgtt tcgcaattag aagagttgat tcgaaagtac
    73681 aacgaaactt ttgatgcttt tatggctgaa atcaaaggta gagtggatag cttaaatcaa
    73741 cagattactg atttaactgg tcaagctaaa acgctacaag acaagttaga tgctctgaaa
    73801 gaagaaattt ctaagttagg taacttacaa gtgatgtaca gtaacagcat cgacttcggg
    73861 gactatgatt attcggagaa cccgaattta atgccatata ttacagaacc ttgggttgga
    73921 cctttgttag gtaatggaca tacagttaaa gatagtgtta aaagggttat aactcatact
    73981 aaaactagaa cagctaattc cggcgacata ttatcgcttg gcttaggtat tccgtgtaca
    74041 gcggaagcta ataataggta tcttataacc actttacgac catctacgac atacacatta
    74101 agcgttacta tgagtgtcgg ttcagattgg actggagaga ccaatactat aggggttagg
    74161 cttagatatt taaatgaaca aggtggtata gaactgccca ttaatgcatt aataccagca
    74221 aatgtagaac gcgataaaat ggtcactcat acatttactg gtataacaaa agataatgta
    74281 actagcataa caaactgtta tgttgaaata ttctcactta atagtgaata taaaggaacg
    74341 gttagtgtca gctatgacgt taagttaaaa gcacactacc ctaacttact agatggccct
    74401 tactggttag gtaaagttcc tttaggcgag aatattgctg accctacagt tgtctttcca
    74461 cacaaaacta gtgaatatat ggtttatggt agacgcaata cagaaaacta tatcgctgac
    74521 caaacatata ccatatcaat gaaagcaact aaactaacag ttcaatcgtt tgccgtgtat
    74581 attgctgctg gccgcgttaa agtaggagac atgaagccta ctgaggggtt agctaataca
    74641 tgggagttaa cttttactgt aactaagcaa catatagata gtggggtaac taattatctg
    74701 gaaatatacc aatacccgtc agctacaaaa ggagctgttc aaattgagtg gctcaaacta
    74761 gaaaaaggca atactcgaac cccgaatatt agtgagtata aatatcgtgg tactggtatg
    74821 cgtgattcaa acaatccaaa agattatgtt tgggatctag caccagaata tgtcgaagat
    74881 aatttggcca cagatattaa aatttctgaa attactggta aagcaaacaa ttataccgat
    74941 gggaaagtat cggagattaa ttcgcagttg actgcttcaa ttaatgaagt agacaccaca
    75001 gctaaggatg ctcaaacaaa agcgaatgct aatgcgactg ctatagatga attagacaat
    75061 aagatcgatg aacgcattaa tgatacagct actaccacat taacagttac aaacgggaat
    75121 accggatcag caaagcttta tcgtgaagga aaaacagttt ctatatattt tgtggcttta
    75181 aacggaaaaa gcagtggtgg aaatgattca acgatactaa caattccaga aggctatcgg
    75241 ccaccaatta gttttgagca actggttggc tcgatagacc gttctacttt gaacagtgct
    75301 cagttatcta ttggtgcaga tggagccatt aaatggcgaa gaaactcaag ttatggatcg
    75361 gattatacct ttgcaattac ttacacgatt tagaaaagcg tgaatcgata tgaaggcagc
    75421 atatagacca attgaacctt acggattcga gcaaatcatt gtgaatgatg aagaacattt
    75481 accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga aattcaatta
    75541 ctgggaggga aataaatgaa aaacatttgg aaatatggac gtactggcgg agagtacgca
    75601 ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc accgcttgaa
    75661 ggaattcgtg ctgatggcga accgttaaca atcgctgatc aaatgtttga tcctaaattg
    75721 aatcaatgga ttattttagc gaacgcacta gatcacaacg atttaaacaa tctcaaagca
    75781 atgtatgagt cgttagaaaa tgagaacggc gatttaaaac agatcaatgc caaactcatg
    75841 ctaagcgatg tagcaattaa acaggaaaat actgcattga aagaaaaagc ggatagttta
    75901 gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga catttcagaa
    75961 attaaagagc aactaaatcc agcttcaaag ggaggtgagt agtatgttta gttttagcga
    76021 tgtgaaaatg atgtatgatt ggggctgttt cactaacgaa caagtaatgg ttttcgttcc
    76081 gttgtgcatt actgaagaaa aagcagataa aatcattagc aaagaagaga gcgcatctta
    76141 attgatgtgc tttttatttt gattcaagga gttgtcacat gattaattta ggggaatggg
    76201 gagcgatagc aggatcaata accgctatcg tttctttgat tttattagta ataaaaccaa
    76261 ttactgcatc tttctcgaag attactgaga ctctttcaaa agtaagtcac aatttagatt
    76321 tgctgactaa agatttagaa tcgagcaaat cagatcgatt gatgattcat gaagaactaa
    76381 agaaacacga tgaaagatta gatacacatg cagaaaaatt ggtagaacac acgcaacaaa
    76441 ttaaaacttt atttagggag agaagaaaat gaataataaa acgttcgaag tactaaaatg
    76501 gttcgcactg gtaattattc ccgcactagc tactttcgtg gggttagttg gtaaagcgct
    76561 caattggcag tacacagata tctgtgttgt catcattact ggttttggcg cgtttttagg
    76621 gagtgtgttg ggtgtatcaa atcgaaccta caaaatgttc tcggctgaaa gcgaagaagg
    76681 aggaaacaaa tgaaaaagaa aattactatt actgcgatga gcctgttaac ggctcttttt
    76741 ttattaccca ttaatacgtt tgcttatact attaatgacg agtataattt agcgccgaat
    76801 caaggagact ccagattagc aattcctaac aaaattattt tgcacgaaac tggaatagat
    76861 gcaccagcaa gaaacgtagc cgccaacatg aaaaataatt ataacggaag caattcttat
    76921 actacagatg ttattggtga cggtgggatt gtttaccgtg tgggtgagca aggatatgtt
    76981 tcgtggggag ctggtaacgc taatccttat gcgcctgtac agattgaatt acagcgcaca
    77041 tatgataaag cattgtttga aaaaaactat cgagcttata ttgaatatac aagagatagt
    77101 gcaaaaaaat atggaattcc attgactctt gatcaaggaa cttctttatt tacaaaagga
    77161 atcatttctc atttgtgggt gacaaattat gtttggggga accacacaga tccatatggt
    77221 tacttatcgc aaatgggagt tagcaaagaa aagcttgctt atgatttagc tcatggattt
    77281 accgatgaaa atccaacaac ttctgaaaac aagcctgtca ttgatccaac acgagctggt
    77341 gcagctaatc ctacgctgac agatggaaca aatcacgccc acattgatca gtttggggaa
    77401 atcgaaaacg caaacttgca tgtggctgga tggcacattg ctaactataa atacgagtat
    77461 attttcatta tggactacaa tactgggaaa gaattagctc gagtaagagc tgatggaatt
    77521 tatagaccag atgtaaacca agcttataat acttctggaa atgttggtta tcatgtatct
    77581 ttcaatatgc gtaattttcc tagtaagaaa gtctatgtaa tgatgcgggc aacgaatgat
    77641 ccagagggaa acactaaagg cggtgcgcaa gatttccatg acaaacgttg gtatttaaat
    77701 attcctaaac gataaaaata gctcctcgtt gaggagcagt acataactat attgacaact
    77761 ataaaaatta ttcgataaaa tagtgatgtt atcgcatatc ttcactatca cccat
//
LOCUS       DASOUD010000003        72758 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.003, whole genome shotgun sequence.
ACCESSION   DASOUD010000003 DASOUD010000000
VERSION     DASOUD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.003"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(193..1560)
                     /gene="nhaC"
                     /locus_tag="U4A29_000179"
     CDS             complement(193..1560)
                     /gene="nhaC"
                     /locus_tag="U4A29_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEO0679334.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1670..3091)
                     /locus_tag="U4A29_000180"
     CDS             complement(1670..3091)
                     /locus_tag="U4A29_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0679335.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3334..5211)
                     /gene="tdc"
                     /locus_tag="U4A29_000181"
     CDS             complement(3334..5211)
                     /gene="tdc"
                     /locus_tag="U4A29_000181"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEO0679336.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5502..6758)
                     /locus_tag="U4A29_000182"
     CDS             complement(5502..6758)
                     /locus_tag="U4A29_000182"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO0679337.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6870..7075)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7338..8639
                     /locus_tag="U4A29_000183"
     CDS             7338..8639
                     /locus_tag="U4A29_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO0679338.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8708..9307)
                     /locus_tag="U4A29_000184"
     CDS             complement(8708..9307)
                     /locus_tag="U4A29_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO0679339.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9561..10442
                     /locus_tag="U4A29_000185"
     CDS             9561..10442
                     /locus_tag="U4A29_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0679340.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10455..12065
                     /locus_tag="U4A29_000186"
     CDS             10455..12065
                     /locus_tag="U4A29_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEO0679341.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12142..12801)
                     /locus_tag="U4A29_000187"
     CDS             complement(12142..12801)
                     /locus_tag="U4A29_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO0679342.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13084..13749)
                     /locus_tag="U4A29_000188"
     CDS             complement(13084..13749)
                     /locus_tag="U4A29_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEO0679343.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13896..14501)
                     /gene="dhaL"
                     /locus_tag="U4A29_000189"
     CDS             complement(13896..14501)
                     /gene="dhaL"
                     /locus_tag="U4A29_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO0679344.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14529..15539)
                     /gene="dhaK"
                     /locus_tag="U4A29_000190"
     CDS             complement(14529..15539)
                     /gene="dhaK"
                     /locus_tag="U4A29_000190"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO0679345.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(15514..15960)
                     /gene="dhaM"
                     /locus_tag="U4A29_000191"
     CDS             complement(15514..15960)
                     /gene="dhaM"
                     /locus_tag="U4A29_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO0679346.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            16063..16383
                     /locus_tag="U4A29_000192"
     CDS             16063..16383
                     /locus_tag="U4A29_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEO0679347.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16495..17364)
                     /locus_tag="U4A29_000193"
     CDS             complement(16495..17364)
                     /locus_tag="U4A29_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO0679348.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17460..18763)
                     /locus_tag="U4A29_000194"
                     /pseudo
     CDS             complement(17460..18763)
                     /locus_tag="U4A29_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(18760..19269)
                     /locus_tag="U4A29_000195"
     CDS             complement(18760..19269)
                     /locus_tag="U4A29_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679349.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19444..21579)
                     /locus_tag="U4A29_000196"
     CDS             complement(19444..21579)
                     /locus_tag="U4A29_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO0679350.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21662..23317)
                     /locus_tag="U4A29_000197"
     CDS             complement(21662..23317)
                     /locus_tag="U4A29_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO0679351.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23319..23633)
                     /locus_tag="U4A29_000198"
     CDS             complement(23319..23633)
                     /locus_tag="U4A29_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO0679352.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23803..24063
                     /locus_tag="U4A29_000199"
     CDS             23803..24063
                     /locus_tag="U4A29_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEO0679353.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            24077..24391
                     /locus_tag="U4A29_000200"
     CDS             24077..24391
                     /locus_tag="U4A29_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEO0679354.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(25121..25675)
                     /locus_tag="U4A29_000201"
     CDS             complement(25121..25675)
                     /locus_tag="U4A29_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEO0679355.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(25801..27729)
                     /locus_tag="U4A29_000202"
     CDS             complement(25801..27729)
                     /locus_tag="U4A29_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO0679356.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(28035..28751)
                     /locus_tag="U4A29_000203"
     CDS             complement(28035..28751)
                     /locus_tag="U4A29_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO0679357.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(28752..30575)
                     /gene="glpO"
                     /locus_tag="U4A29_000204"
     CDS             complement(28752..30575)
                     /gene="glpO"
                     /locus_tag="U4A29_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO0679358.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(30589..32085)
                     /gene="glpK"
                     /locus_tag="U4A29_000205"
     CDS             complement(30589..32085)
                     /gene="glpK"
                     /locus_tag="U4A29_000205"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO0679359.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(32221..33681)
                     /locus_tag="U4A29_000206"
     CDS             complement(32221..33681)
                     /locus_tag="U4A29_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0679360.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(33671..34966)
                     /locus_tag="U4A29_000207"
     CDS             complement(33671..34966)
                     /locus_tag="U4A29_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO0679361.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(35298..35975)
                     /locus_tag="U4A29_000208"
     CDS             complement(35298..35975)
                     /locus_tag="U4A29_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEO0679362.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(36005..37096)
                     /locus_tag="U4A29_000209"
     CDS             complement(36005..37096)
                     /locus_tag="U4A29_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO0679363.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(37197..37631)
                     /locus_tag="U4A29_000210"
     CDS             complement(37197..37631)
                     /locus_tag="U4A29_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO0679364.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(38215..39672)
                     /gene="ascB"
                     /locus_tag="U4A29_000211"
     CDS             complement(38215..39672)
                     /gene="ascB"
                     /locus_tag="U4A29_000211"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO0679365.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(39669..41564)
                     /locus_tag="U4A29_000212"
     CDS             complement(39669..41564)
                     /locus_tag="U4A29_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO0679366.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(41622..43397)
                     /locus_tag="U4A29_000213"
     CDS             complement(41622..43397)
                     /locus_tag="U4A29_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEO0679367.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(43447..44943)
                     /locus_tag="U4A29_000214"
     CDS             complement(43447..44943)
                     /locus_tag="U4A29_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO0679368.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(45137..46759)
                     /locus_tag="U4A29_000215"
     CDS             complement(45137..46759)
                     /locus_tag="U4A29_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO0679369.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(47208..48521)
                     /locus_tag="U4A29_000216"
     CDS             complement(47208..48521)
                     /locus_tag="U4A29_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO0679370.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(48766..48990)
                     /locus_tag="U4A29_000217"
     CDS             complement(48766..48990)
                     /locus_tag="U4A29_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679371.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(49010..49432)
                     /locus_tag="U4A29_000218"
     CDS             complement(49010..49432)
                     /locus_tag="U4A29_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEO0679372.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(49575..50285)
                     /locus_tag="U4A29_000219"
     CDS             complement(49575..50285)
                     /locus_tag="U4A29_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679373.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(50303..50800)
                     /locus_tag="U4A29_000220"
     CDS             complement(50303..50800)
                     /locus_tag="U4A29_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0679374.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(50837..51289)
                     /locus_tag="U4A29_000221"
     CDS             complement(50837..51289)
                     /locus_tag="U4A29_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO0679375.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(51424..52029)
                     /locus_tag="U4A29_000222"
     CDS             complement(51424..52029)
                     /locus_tag="U4A29_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO0679376.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(52479..53099)
                     /locus_tag="U4A29_000223"
     CDS             complement(52479..53099)
                     /locus_tag="U4A29_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO0679377.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(53101..53970)
                     /locus_tag="U4A29_000224"
     CDS             complement(53101..53970)
                     /locus_tag="U4A29_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEO0679378.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(53993..54400)
                     /locus_tag="U4A29_000225"
     CDS             complement(53993..54400)
                     /locus_tag="U4A29_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEO0679379.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(54419..55708)
                     /locus_tag="U4A29_000226"
     CDS             complement(54419..55708)
                     /locus_tag="U4A29_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0679380.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(55735..56037)
                     /locus_tag="U4A29_000227"
     CDS             complement(55735..56037)
                     /locus_tag="U4A29_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0679381.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(56062..58155)
                     /locus_tag="U4A29_000228"
     CDS             complement(56062..58155)
                     /locus_tag="U4A29_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO0679382.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(58644..59408)
                     /locus_tag="U4A29_000229"
     CDS             complement(58644..59408)
                     /locus_tag="U4A29_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEO0679383.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(59519..60388)
                     /gene="mdcB"
                     /locus_tag="U4A29_000230"
     CDS             complement(59519..60388)
                     /gene="mdcB"
                     /locus_tag="U4A29_000230"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEO0679384.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(60413..61489)
                     /locus_tag="U4A29_000231"
     CDS             complement(60413..61489)
                     /locus_tag="U4A29_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO0679385.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(61513..62106)
                     /locus_tag="U4A29_000232"
     CDS             complement(61513..62106)
                     /locus_tag="U4A29_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEO0679386.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(62110..63786)
                     /gene="mdcD"
                     /locus_tag="U4A29_000233"
     CDS             complement(62110..63786)
                     /gene="mdcD"
                     /locus_tag="U4A29_000233"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEO0679387.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPLDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(63776..64075)
                     /locus_tag="U4A29_000234"
     CDS             complement(63776..64075)
                     /locus_tag="U4A29_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEO0679388.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(64177..65823)
                     /gene="mdcA"
                     /locus_tag="U4A29_000235"
     CDS             complement(64177..65823)
                     /gene="mdcA"
                     /locus_tag="U4A29_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEO0679389.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(65830..66741)
                     /locus_tag="U4A29_000236"
     CDS             complement(65830..66741)
                     /locus_tag="U4A29_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO0679390.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            66861..67718
                     /locus_tag="U4A29_000237"
     CDS             66861..67718
                     /locus_tag="U4A29_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO0679391.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(67905..68267)
                     /locus_tag="U4A29_000238"
     CDS             complement(67905..68267)
                     /locus_tag="U4A29_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO0679392.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            68613..70052
                     /locus_tag="U4A29_000239"
     CDS             68613..70052
                     /locus_tag="U4A29_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0679393.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(70059..72599)
                     /locus_tag="U4A29_000240"
     CDS             complement(70059..72599)
                     /locus_tag="U4A29_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEO0679394.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQSLQQT
                     ILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPVGQQL
                     IGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
BASE COUNT        20671 a        15437 c        12541 g        24109 t
ORIGIN      
        1 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
       61 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
      121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
      181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
      241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
      301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
      361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
      421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
      481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
      541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
      601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
      661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
      721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
      781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
      841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
      901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
      961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
     1021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
     1081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
     1141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
     1201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
     1261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
     1321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
     1381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
     1441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
     1501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
     1561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
     1621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt tatttttctt
     1681 ctaaactttt ttctacctca atcccccaag aatctttctt gaagtgattg atgatcaatg
     1741 gaataacgaa catgaccact gtgatagctg ctactaataa tgcatcactc aatgtcgctg
     1801 tacctaatgt agcaaatacg acaacgatga tagagaagat cagcatgcag ctgactaccc
     1861 aagctaatcc gtttccgttt ttaccaattc ggtaaggacg ttcgaattct ggatgtttct
     1921 tacgcatttt aagcaatgaa acagcaatca aaatgtacac gatacaataa ataacagttg
     1981 tagcatttgt aagtgtcaag aatacagcac ttacgtcatc cattacacca taaagcaaag
     2041 caaataatga aataacgatt gattgtgtta gaacgacatt tctagatacg ccgtatttgt
     2101 tttctttatg gaatccgaat tttggtggaa ggaatccttc acgagctact tggatgattg
     2161 tttttgatgg tccagtaacc caagctgata attgtaaaag cacaccaata aataccatga
     2221 agctgaagat atttccgatc acttctggta agcctaggat ttgacagtca atcaagatag
     2281 gctgcgtgat atttgctaat tccatttttc catctggtac gacatttgct acaagcatcg
     2341 cattgatgac attcaataaa actaatccga tcaaagcgat aaatacgcct tttgtataat
     2401 tttttgttgc gtctttcaaa cgaggcatgt aaacagaact catttcgatc ccaacaaaaa
     2461 taaatgtgat accagctaag tatttcaatg aatcaatatg ttccaagtca gggaaagctt
     2521 ttgaccatga aaagtcacct agatagccgt ttgagactag tccgactttg aaggctgcca
     2581 aaacacctaa aacgaacatg atgactactg ggatatatac ccctagccat acaccgatgt
     2641 tcccgccgac ttttgccata tcgaatttca agttcaagat cgtgatgatc cagtagataa
     2701 ctaaaataca acctaaaacg aaccagtgat tattgccaag ttctacatta ccaaatgtgt
     2761 ttcctaacaa tggtcctaaa gtagaagcga ccattaccat acctgggaac atttgcaccc
     2821 ataatagcca tgcaacgaca aatccccatt tgctgcccag tgctgttttt acccataact
     2881 gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc ggaaaccatt gatatcggac
     2941 cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta
     3001 acgtaggaat actccgaacc gttgcacaca gtgccattgt cagaccgata aaggtcccaa
     3061 gacccatttt acttacttca gatttactca ccgttgacac tctccttgat tttttggata
     3121 aaataacggt gtctttgaag tagtttcagc aataaacgaa acaattcagc gaatatttga
     3181 ggggattctt tgagtctatt gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg
     3241 atcgaagagc ttaggtctcc ccttgctctt cagatccaaa catgcgtcag aaacagttag
     3301 tattcaagct gttttctaac atgattacct tgcttatttt gcttcgcttg ccaataattg
     3361 gtctgcgtag attttttcca atttttcttg aagagctgct ttgatttttt cagcatattc
     3421 ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg ctggcacgca atacagttac
     3481 ttttccggca cggttccatt cttcatctga gaatcctagt tggttcacaa attgtaatgg
     3541 gctgttgcca tagtctggga tagcaaagtc agtatgtgaa gttaagaact cattaccgta
     3601 aatgcttcct ttcacgtatg aagaataatc atatacgtca tggttcaatt tattcattgc
     3661 aactaagtca tcgttgcctt tttctttgaa tacatagtct accatattga aatctggata
     3721 agttaatgga tgaacttcga tttctttgtc tcctacttta aatgacaagt cattcaagaa
     3781 gttgtagaaa cgatgtgcac cttcgattga tgcacccatt aatttaccat atcctgtgac
     3841 attcaaaggt aagacatggt gtgctgccca aacgctggct gctgttgccc ctgcttttga
     3901 accttccaag atataagccc caagtaatgc tgggatatct gcgccttttt caaatacata
     3961 tgtcgcaaag taagagataa cgtctctcat gcggatatct ttgatgacga taccaccggc
     4021 agaatatgga acatagccca ttttatgtgg gtcaatcgtt acagattctg cttcttcgat
     4081 tgctttatat gcactgtgta cttcttctaa gatataatct ttgttttctg taaagacatt
     4141 gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa
     4201 gaaaatcgca cggccgtatc cgccataagc agcatctacg tgtaagtaga agtaaatacc
     4261 gtctttttct aatacgcgac gcaatgcaac gattttatcg ataccgtcga ttgctccttc
     4321 ttctgttgat cctacaacac cgactacacc taggattggt gttttttcag ctgctagacc
     4381 acgaacgatt ttttcaagtt cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg
     4441 aatcacttgg tctaaaccga taccgatgat gtcggcagct tttagccatg aatagtgttt
     4501 agtttgtgga accaaccatt ttcctaattt ttctaaatgt tttccgctac gtgctgagtg
     4561 agctttgatt tcgtcgattt tttctgggac gctgtctaac agattcatga tttcttctgt
     4621 tgacaagttc attaattccc aatcgctttt tccagcaact agttcaggtg tcacttcttt
     4681 cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa agtccttcta agtttgctaa
     4741 agaaccatca gcaacgatgt ggccccagcc atctttatag ctcattaatt ttgcaaattc
     4801 cattcctact tcttcttcca tttggcttgt tgcaggagaa gattcatagg caacgttatt
     4861 accattccat agcatagcaa aattgtacgc tagtaaagat ggcatcaatg tttcagagtt
     4921 catatgaccc cagtaacgtc cggcattatg ccaaggaacg gaatgggtac gcatccgcgc
     4981 tgaaatttct gatagaacat cctttgtttt attcacagtg tgttcaaaac tagcactgct
     5041 tttttcttct ggcgtaataa taggcatgtc ttgaggcatg tagttttgac gccagcctaa
     5101 atgttcatcc accaattcat tcaacaaagc tttatagatt tggccgttct cagctttgtc
     5161 cccaatgaat agggcattta ggtttagatc tttcgacaat gattcactca tagtgaaacc
     5221 ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta cacctgttct tctattctgt
     5281 aaactaagtt ttggcctgat ttttaatcat attttaatat gtatcaatta tccgaattgt
     5341 tcacactttt gaaataatta aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa
     5401 agattgtatt aacgacaacc agcgttctat tttaggatgg gtttcaaaat tggaatgttg
     5461 taatagtatt tccctttttc aaggtaaaaa cttactaaat tctaatcaat tactttggcc
     5521 aaaaagtaat ttttcttacc ttttcgaata accacaaact taccatcgaa ttcatcggat
     5581 ggatttatga caaaatttaa atcggtaacg cggtcaccgt taatacttat agctccgttt
     5641 gaaacatctt cacgtgcttg acgtttagat ggttcaattt ttgtggaaac aagcagttcc
     5701 acgatatttt caggtgtgct tgaaatttca acgttcggca ttttaccgaa tccttgcgcg
     5761 atttcttccg catttagttc tttaatattt ccagagaaca atgcttgcgt tattttttgc
     5821 gcttctttca agtcttcttc actatgcaca aatcttgtaa cttcttctgc caaacggcgt
     5881 tgtgcttcac gtttctcagg ttctgtctct acttttttcg ctaagtcttc gatttcttct
     5941 tgagacaaga aagtaaagaa tttcaagtat ttcactacat cgcgatcatc ttgattcaac
     6001 cagaattggt agaactcaaa tggtgaagtt ttcttcggat caagccaaac ggcaccgcct
     6061 gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt taaaccgaac
     6121 gcttctactt ctggtccttc taattttcgg attaggtcta atccagctgt gatatttccc
     6181 cattggtctg ctccaccgat ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag
     6241 tcgatagatt gcagaatttg gtaagtaaac tctgtaaatg agattcctac ttccaaacgg
     6301 ctggcaacga tatcttttgc taacatcgtg ttgatgttga agtttttacc gtagtctctc
     6361 aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt
     6421 gcatctttac caaacaattt tctcatttga ttggaaagtg cgtccacgtt atgttgcact
     6481 tgttccatcg tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct
     6541 gttccgccgc caatcagaat atatggatga tggcctgcca attggaatct tttcatcatc
     6601 ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac gccgcaataa
     6661 agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta
     6721 atggcgtcgc gccaagttaa ttcatcaata atattcattt tactgacctc cgtgtagttt
     6781 ttttatgaaa gcaggaacct gctagcatgt gtaattaatt atttcttcag gataggatag
     6841 gaaataaaaa aagtcccttg gcatataagc caagggacga aatctcgcgg taccacccaa
     6901 tttgtatgtc tagatatcca acatacctct cgaacattta acgctgtcgc gtggcaatca
     6961 ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg tactggtttg cagcgaccac
     7021 cagctttctg aaacattggc tttaacaact acttcgtttc gatcttcgtc ttgttattat
     7081 taatatgtgt ctatcataga gcgcaacttt ttctttgtca acagaaaaag gatatttttt
     7141 ttaaattcct cattttttct tttttgaatc cgctctaatt ccgaacattt tattttcaat
     7201 tattgttaaa agaaataact ttttctgtat tttttctgca atttatcgag aagtcacttt
     7261 actttgcctc ttattctttc ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa
     7321 agcaagggac gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc acttctgctt
     7381 taactatcgt tattttaatt ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag
     7441 aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca gatgataagc
     7501 aacaactagt tactcaagca aaaaatctgg ctcaaagcta cgattatgac caagcgattg
     7561 cgttattgga aaaagatagt aagaacggat ctactgaaac acaacagctt cttcaaacac
     7621 taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc tactcagatc tctcatgtct
     7681 ttttccacag cttgatcgtg gacccgcaaa aagccttcca ctctcaacag gcacaaggat
     7741 acaaagatta tatggtgact gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa
     7801 atgattttgt tttagttaat ttaaatgatt tgatccaaaa agacgaacaa ggaaatcttt
     7861 cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca caagatgatg
     7921 tcagctatta tgaatatatg gataattccg gttttcctag ccgattggtc gtatcaaata
     7981 acaatgaaat caaaaatgtc tataaagaga aagataaaga agaaatcggt gactttgaca
     8041 tggttcctct gatcgatgcc tttgtcaaga aacatcctga tttttcttat cgaggcgcaa
     8101 aaggtacctt ggccctgaca ggttacaacg gagttttagg ttatcgaaca tcaaaatctc
     8161 aatatggaga caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa gtggcggaac
     8221 agttgaagaa agacggctgg agctttgctt cccatacatg gggtcacttg aatatgactc
     8281 aagcttcact agaagatgta aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta
     8341 tcctaggaaa aacaaatatt ctgatttatc catttggtgc agacatcagc gactggcagc
     8401 cttattcagc agaaaatcaa aaatttgctt acttgaagag tcaaggtttc gatattttct
     8461 gtaatgtcga tgcttctaca ccagcttggg gacaattagg tacggatttc tatcgaaatg
     8521 ctcggatcaa tatcgatggc attcgttttc aatctgattt agacggaaaa aatcctattc
     8581 ttgatccatt catcaatgtc catgatgtct atgacaaaga agcacgcgaa tctgcttgat
     8641 tttacgtcat ttatgaacca aaaaagccga actgacattt tatcgtcagt ttcggctttt
     8701 ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc cattctgctg
     8761 tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc attgcttgaa
     8821 aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct ttccgccctc
     8881 gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc atcgcgtcag
     8941 aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa aattgtttgt
     9001 tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt tcttcaaatg
     9061 gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata aatgcaaaaa
     9121 atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc gctttactta
     9181 cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg tctgtgttca
     9241 tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc ttttcaaaac
     9301 cgttcatttg tagagcctcc cgtaatttta aacttttata atatatatag ttcaaattta
     9361 ttctgaggtt tattataact atctattgag aaagtatcaa gctagcaatt ccttgtttat
     9421 gaattcattg acagaaattt ataaaaagaa tattatgtac taaaataata aaaaaagttt
     9481 tataaaaagg tataaaacct ttttattaaa ctatttaatc ttgtttagta tatattttta
     9541 gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg aaaaagagct
     9601 ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt gaagtcgttg
     9661 gatttatcgg accaaatggt tctggaaaat ccacgacgat tcggacactt ttagggatta
     9721 taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa gatagtttat
     9781 ctatccatga acgtctttct tatgttccag gcgatatctc tttgtgggga aatttgactg
     9841 gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag
     9901 atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag
     9961 gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat ctctatttat
    10021 ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa gaagaagttg
    10081 aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc agtgaagtcg
    10141 agcgactagc ggataaagtc gtgatcatac gcgaaggaac agtcgtagaa gaaggaacac
    10201 tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa gcagagaata
    10261 gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat
    10321 ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga
    10381 aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat tatgaaggat
    10441 gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt tctcaaaacc
    10501 ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct ttttctggtg
    10561 gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga ttatacgaaa
    10621 ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg aatgctgctg
    10681 agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg
    10741 ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga
    10801 ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg
    10861 aaagtattct gatcaatttg cttttagcaa tatcgatcag ctgtacgatg atttttttcc
    10921 aagcagattc gatcactgca gaaggtgctt ggctttttgg gctatctgta ggtagtatgg
    10981 ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt tcagccagtt
    11041 cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca ataacagatg
    11101 tagctgattc ctccctttcg atgtggaatc ctttaggctg gtcttatttg acctatccat
    11161 ttacaactaa caactggatt cctttgcttt atttgttttt gttcagtcta atcttgataa
    11221 tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc cccgaacgtg
    11281 aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta ttctatctaa
    11341 ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg gcatacggct
    11401 ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa cagatgttta
    11461 tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggtc gtcttgattt
    11521 gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa gaaacccacg
    11581 gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat tggaccaata
    11641 tgttactagc tattctattt gctatattgg caatcctgat tagttctggt gcactagggc
    11701 tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg tttcttaaag
    11761 ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc tttctattcg
    11821 gatggcttcc tcgaatgggt aaacttgctt atatctactt agcttactcc tttgcactca
    11881 attactttgg cggaattttg gatttaccag aatggttttc caaaacagct gtccctagct
    11941 gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg ttatcatgta
    12001 tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac ttactagaag
    12061 gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca aaaatccaac
    12121 atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa tgatccagac
    12181 ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt ttctagataa
    12241 agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg gcaggaaaaa
    12301 gtgatccttc cctgtcgctt tagctaagtc aagcagatag acagcgactc gttcttctac
    12361 tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct caacgttgat
    12421 tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga aatctttttg
    12481 tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact catttggaac
    12541 gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta aaagctgttc
    12601 ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga tcactaattg
    12661 aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat gtttgacaag
    12721 tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa caaatgaaac
    12781 acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc taataaagaa
    12841 acataacttc ccttattcca actgataatt ttcttttatc atagcataaa cgtcaagcga
    12901 accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt tccttctttt
    12961 gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg aataatcaaa
    13021 aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt
    13081 tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt cttgtaaggt
    13141 ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata gtttacccat
    13201 ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg tatcggacaa
    13261 atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata
    13321 atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact ggttatacgc
    13381 tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat agatttggta
    13441 ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag attcgcttcg
    13501 gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt cgattttagg
    13561 tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa tctctttcca
    13621 tttttgccag tgttctttat attctttctt gatttgttca atcttatctg ttggatggct
    13681 ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat ctgattttcc
    13741 aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat tttgcgatat
    13801 ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca gccaaacaaa
    13861 aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat cacatctaaa
    13921 agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc gattgcacgc
    13981 tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg ttcttttgcc
    14041 tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac tggcagccaa
    14101 acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat acccgctgtt
    14161 ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac ctcttttgtc
    14221 gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc accgactttc
    14281 gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg atgatcggct
    14341 tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg gtctccatcg
    14401 ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc ctctgcaaat
    14461 tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc cttttatctt
    14521 gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc
    14581 tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt cataaaatta
    14641 ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa aacatcattc
    14701 atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag tacacctgct
    14761 tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt tgccagtact
    14821 ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc aacaccatac
    14881 tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac agttgctgct
    14941 cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag ttctttcaac
    15001 tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac aaggacagta
    15061 cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat atcatcatct
    15121 acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc catatcgaag
    15181 ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc actatccgct
    15241 tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc accaagcacc
    15301 gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg tccgcttcct
    15361 ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac acgggaagat
    15421 tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat ttcttcgaca
    15481 atattgcttg gatcattcat gatttttttc aatttattcc catcctttct ttccggcatt
    15541 ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga tggctgctgc
    15601 aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct gctcttcttc
    15661 caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa
    15721 ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg ggtctgatcc
    15781 gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt tttgcatctg
    15841 ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa gcaaaatact
    15901 ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt tcgctttcat
    15961 taatgaacaa ttcaagtata taataaattt ctaattttgg gaagatatgt gtagtgcttc
    16021 ttgaagtttg ttatacttta attaaaagag aggagtgatc gaatgattac aacaacaacg
    16081 aacagtgtag agggaaaagc cgtagtagct tataaaggga tcgtttttgg tgaggtcatt
    16141 actgggatcc atgcatttaa agacttggag gcggggttgc gaaatatctt tggcggccgc
    16201 tcaaaaagct atgaaaacga actaatgggt gcaagagaag aagcacttgc cgaaatggcc
    16261 gaacgtgcaa aagcattagg agctgatgcg atcattggcg taaaaatgga ctatgaagta
    16321 ttaggcagcg acaatggcat gctgatggtc acatgtagtg gaacagctgt ctccacgaga
    16381 taagtagggg acatctgttt taataactaa agagagatgg gacatgtagt ggcactcaag
    16441 aattagattt ttcagtctaa cttcgaggta ctctaccttc ccatcctctc ttttttattt
    16501 ctttttgcgg atgattccgt aagctgctgc gactaacaca cccgcccctg ctaaggaata
    16561 actgacagaa gctgtttttt catttgtttt tggcaaagtc tgacttgttg atttggatgg
    16621 ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag gtcactgatt tttctcctgc
    16681 cgaatgcaaa atatctttta ttcgttggaa actagctgct aagtcagctt tttttgcttc
    16741 aaaatcatta gcaactgttt gtgcttcgct gatctttccg tggattgctt gactggccgc
    16801 tgtgatatcc gcttgagttt gacttagctt tccagaagct gtttgagcaa cctgatttaa
    16861 ttgctgattg aattcttttg cttgttggat cttcccgtca agatctgttt ggacagcttc
    16921 cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat ttcccacttt
    16981 atttttaaga tcagctaaat cttgatcgat tgctgcttgg tcttctttca atttgttttt
    17041 tgcagcttct aatgcttctt cgagttcttt tcttttattt tccaagtctt gcaaatgatc
    17101 gatttggtca gaagaggtag aactatttat tgatgtatct tctgaagatg aaatcgtttc
    17161 tcctgaatta tctgtcggta cattggaagt catttctgtt tccgaggcac cgttttcagc
    17221 accagaagag cttatagttg ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa
    17281 agctgtctgt cccaataaga aaatactgga aacagcaatt ggccagatta acggcttcat
    17341 tctttttttc atcacttttc ccatccttac tactttttaa ctaatttctt attataagca
    17401 aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt
    17461 tatttttttg tacgttccgg gcttttccat gtcccgcttc cataacctaa aatcgttttg
    17521 actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa caaaagcata
    17581 tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc gatcatctac aatcagcgaa
    17641 gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta
    17701 aaagtaatat agatctgttc gtagtttcct tgatatacat aatgcccgat taatccagca
    17761 aaaagaacta cggaaataca aaagaatatt gaccagacaa tactcaatgt ttgatcaatg
    17821 aagatgatcg ttcttccaat atgtttaaaa ggatggagca tcactttttt gaaattcgtc
    17881 agccagactt ctgttccacc ttttgcccaa cgttttcttt gtcgatatag catcttcaac
    17941 gtcacaggta cttccatgaa aaagataatc tcaggagcaa aaacagataa ccagtcattt
    18001 aactgatgat cccaagcaat actgatgtct tctgtcgctc gatcttgtct gaatccgccc
    18061 acatcgatca aggcttcttt tcgatacatc gtatttgctc cactgtacgc ataaagacca
    18121 ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt
    18181 tgagattttg caatcaattt ggtacgattt tggacatcca tatttgcagt gaccgcagca
    18241 atatgtcttg caccagggcg aatgaaataa ttcacatacc taatcagtgc atctggctcc
    18301 ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc aagtccgata
    18361 ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa ccacacgaag attagcgtat
    18421 tttttcatca gtctagctaa tatttctggt gtttggtccg ttgaaccatc gtctgtcact
    18481 aatacctcat agttgtgata attcaatttc gtcatcaaat actcgattgt atcttcgatc
    18541 acaatttctt cattatgggc tggcaccatg atcgtgatga acggttcgac actcaaggga
    18601 acatctaccc attctttttg cttatgtttg tataaaaaca cataacacca gacaccaata
    18661 aaccatacaa atccgcctaa aatcggataa gaaacgagaa aaaatacata atagacaaac
    18721 aagtatctaa gaataaatta cctgtatggg tgattgagat catttcagtt ccaccccatt
    18781 ttctttatac aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg agacagaata
    18841 aaaacggacc gtttctctct cttcttttgt tccaaaacgt tccgtataca cttcttcaag
    18901 taattcttgt ctttttttca atttttcatc ttcatattca aaagatttat ttactttttt
    18961 gtatagattt cgattgttcc acctagtcaa tacaaatgag gtaatcagga aactgaaaaa
    19021 aatcaataca aaaaagccga ttaagaaata cagcattttc ttttctgata aaaaagcgta
    19081 caaggaaaaa tccttggctc tttctggtct cgttaatggg aaaaagaccc atataaaagg
    19141 aagaacaacg atcatccatc ctaaaagagc cactaacgtt tgccaaatct ttaaccagta
    19201 attacctttt gaaaaatact tatctttcac caaaattgat gcaacttctt ctcttgttac
    19261 ttttttcata tcctcacctc acgttttttt gttacgacgt aggagtttac catacatttc
    19321 atttcttttc acataaagtg ttcctaaatt tttcctaaga aaaagaacaa aacatttctc
    19381 atcagcaaat gaaaaatatt ttgttctttt tatcagttgt tctttttatt ttttatctct
    19441 taattatgtt gctctttcca atacgacaat gcttttcatt cctctagata tcgccgtgta
    19501 catttgtctg cgtcttcttt gctcatctcc atcgttttca aatggatata agattacatg
    19561 atcaaattcc agtccttttg ctagactcaa aggaatgatt tggcagtttt ctaatccata
    19621 tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc tcttctgttt gggtgataac
    19681 tgccgctttt tctcctttta gagaaaccaa aatctcagct agtattttca acaattcttc
    19741 agaggatcga aaagagcggt attcgggttc ttttccttgc ggtcggatcg atacgatgtc
    19801 gatcttctct tgatccacag cataggtttt gaaaagctct gtgattgctc cgctagaacg
    19861 gtaactattt ctaagatcat accgatggat cggcagatgg tagtcgtcga aacaacgcat
    19921 aatatcagca aaagtagtgg aagaattaaa gattgcctga ttctcgtcac ctactaaagt
    19981 aaagtgagtt tttggaaata attcgatcaa tagacctaat tgtgccaggg tataatcttg
    20041 tacttcatca atcaatagat agcgaaatgc tggtacattt accttttcaa caagtaaatg
    20101 acgaatagct aatataatga ctgcttcatc aacagtacgt gtgggttgcc aagcataagc
    20161 tgagccatat cgtttttcat aaattctttc aaacaactga tgctcatcca cccatgccat
    20221 ttcttcgatt tgtctggtta tttttcgata ttttttttgt aaaagtttcc ttgcataagc
    20281 tacaatactt tgttccgaat catcagaaat caatttaccg aataattcca actgcctatc
    20341 ttctgtcaac gagagtacct gattctgcac agcaggactt tttgcttgtt tcaaaagatg
    20401 attcttccat tcgctcatca gtgccttttt cgttgcttga agcctatcga tcaatttagg
    20461 atgagcagga actttctcat atattttttc gatcgttttt ttagaaagga ctgtttttcc
    20521 atttttagta agtccacgga taaaattcgg atgatctaaa aacaaaggat ctgattgttt
    20581 tagattatcg ataaactttt tgctacgcaa acgttctgtc tgctccgata cattttctcc
    20641 cgtgatacgt ttgaaataag cctcttcacc ttctatttcc tctgctgaaa gctgtgaaac
    20701 cagctgtatg atggttagat tcagtggatt tctttcacct aatgatggta ggacattcga
    20761 aatatagtcg ataaaccgat gattaggtga tagaatcagc aagtcatcgg atgtcatcgt
    20821 atctcgatat aaatagagca gatacgcaat acgctgcatg atagcagagg ttttaccact
    20881 tcctgctacg ccatttacca agatattttg acttttcaca tcccgaatga ttgtgttttg
    20941 ttccgattgg atcgtagcag tgatagcctt catttcattg gtctcatctt ggcctaagac
    21001 ttctaataat acgtcatctt gaatagctac agacgtatca aaaaacttga ccaaacgatc
    21061 tctttccaaa agaaattgtc gtctttcttt aataaccgta tcgattttat tttgacgaac
    21121 cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt tcggcaattg gtgaacgcca
    21181 atcataaaca agattttcat ctgcatcatc tgtgaaattt gctgtaccga tataaaagga
    21241 ttcttttggc tcattatcca gaaagtctac aacgactttt ccaaagtagg gacttttcaa
    21301 taaacgttcg atctttgcta gctgattttc tgcgtttttc tgtttgatat tcatctgatc
    21361 gatttctcta tttttcattt caatcatcga aaaagtatcc aggttatcta agtaactgtc
    21421 aaaattcaat cgaagctcgc cagccatttc tttcaacgac tggtttcctt ctatttttgc
    21481 cgtatccatc gattcccgca gttcgttttt tttatcaatt agcttttgat acacgttctt
    21541 cagatatgct tcttctaact gtttttgtgt gttgaccata cacattctcc tcttttttta
    21601 aagtggtgga accgattagt ttacctcttt taagagtcga atgcaagctt tttaacgagt
    21661 atcatttgtt aggatatatc agttcttcag gtcggacagt ttgatataaa gaaaatgcac
    21721 catcaaggtt ttttacgtgg tagccagact gcttcaagat tctttccgct agatagctac
    21781 gtaatccgct atgacagctg acgatatatt cttgttggct gtctaattca tttaatcggt
    21841 cccgaagttc attcaaaggg atatgtttgg cttccttgaa tttgcccttt tccaattctt
    21901 ctgctgttcg cacatctaat aaaatttttc cttttgctag ttctgtcggc aattcatgcc
    21961 attgtacggt ttcactaagc ccttctgcaa tgttcattgc tgcatatcct agcatgttta
    22021 ctggatcttt ggctgagcca aatggcggtg catacgtcaa ttctaattcc ggtaaatcga
    22081 agatcgtcaa atgccctttg attgctgttg ctaaaatatc gatccgttta tccacacctt
    22141 ttgccccaac accttgtgca ccataaatct cgccagtctc tggatggaag atcaacttca
    22201 gtaaaatatc tgttgcttct ggataatagc ttgcatgatc ttttcctgat acatgaatga
    22261 cttcaaaaga cagttcgttt tctttggctg ttctttcact taaaccggtg gatgcggcag
    22321 tcattccaaa cgttcgtata attgctgttc caataccgcc ttgatttctt cgagaaattc
    22381 cggaaatcgt gtccgcaact tgtctacctt gacgattggc tggtgaagca agagaaataa
    22441 gtgcatcttt tccagtgatt tgctgtttta caacgatagc atcccccacc gcataaatat
    22501 caggaatatt cgtttgataa cgttcgtcta ctaggatacc cccgcgtatg cctaattcga
    22561 ttccagcggt cactgctagc tggttatcag gacgaacacc aatggataaa ataactaaat
    22621 cagcagggat ggtacgccca tcatccagtc gcaatcgttt cccgtggtct tcaaatccag
    22681 cgacagcatg agaagtaatc acttctactc cttgatgaga caattctttt tctataaaag
    22741 ctgccatttc ttcatccagt gttggaagaa tatgtggtgc ttgttcgatc accatgactt
    22801 ctaaacctct tctttttagg ttttctgcca tttccaatcc gatgaatcct gcaccaacga
    22861 tcacggcacg ctttggctgt ttttctaatg catgcatcac tttatcgata tctggtacat
    22921 ttctaataga aaaaacattg tcggcttctg ttatccctgg aatcgatgga acaactggtt
    22981 ttgcaccagg agataaaacc aatgcatcgt actgttcaat gtgtttttga ccctcatgta
    23041 cgacttctac cgttttgttt tcgggaaaga tggctgtcac ttcatgattt ggacgcacat
    23101 ctaaacaaaa acgagcggat aatgattctg gcgtttggac aagaagattt tcccgttcac
    23161 tgatttctcc cgaaagataa taaggcaagc cgcaatttgc aaaagaaaca tacggtcctt
    23221 tttcaaaaac aacgatttct gcatcttcca ttaatcggcg aagccgtgta gcagcagaca
    23281 ttccacctgc tacacctccg acaataacga ttttcatctt atttccctcc tactgttcga
    23341 tcaaaccatt gattcatgcc accacgtaca ttcactacat cataaccaga tttctttaat
    23401 tctttagctg cttgtttact cctcatccct gattgacaaa tcacatagac ggtcttttct
    23461 ttgtctccat catatctgtt tatactttgt aatggaacat ttttagctcc ttttatatgc
    23521 ccgccccggt attctgcagg tgttctaaca tcaagtaacg ctggattttc caaaagcttt
    23581 tcctttaatt ctgggactgt tatcgatttt attgattgaa atagcaaatt cattttctgc
    23641 acctcattta catttttata aatacctcat ggggtatatt aaacaggagc tttattaata
    23701 tgtcaaccag aatgactctt catttttttc actacttttt atatttgcat aaaataccga
    23761 taaggggtat tatctaatta taaaaataga ggagaatgtg ttatggagat tgataataaa
    23821 aatatcctga accgtttgaa acgtacagag ggacaaattc gtggtatcca aaaaatgatc
    23881 gaagacgaaa aagaatgtat ggatgtcatc actcaattaa gcgctgttcg ttctagtatt
    23941 gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa attgctttga gaaacctgat
    24001 agtaatccag aagaacaagc ggctaaacta gaacaagcga tcaagatgat tattaaaaag
    24061 taaaggaatg atttcaatga aagtcgtttt ccatattgat gaattagaaa aatggtcaga
    24121 aacagggaaa aacgtaaaaa acctgataaa agcttctcca gaaacgacga ttgtcgtatc
    24181 tgtaaatggc atcgcgatta ccggttatct agactctgcc aatgcagaat tcttagattt
    24241 gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt gctaatcata tttcagaaag
    24301 ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc cttgatttag ttgaactgca
    24361 atcccaaggt tatgcttata tcaagcctta gtcagtagag gttgcgacag aagtgatcag
    24421 cttcaagaaa tttcggctta tttttcgaag aagctacttc tggaaaaccg tgaatcaaag
    24481 gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc caaacaaaaa tagcttctca
    24541 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
    24601 agaggttgtg aaatcagcgg tttgacctaa gtattagaga aaaaaacggg aaaatagctc
    24661 ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttaag
    24721 gaagagattg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg tgaaaatgga
    24781 tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt
    24841 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat
    24901 agctgctcat attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt
    24961 ttaccaagag gttgtgagcc taccatttaa ctccgaatca caagaaataa tttcaaaaac
    25021 aacttctcct gtttttggtt tggttggctt attatcgaga agtagctact gggagtctat
    25081 ttatcaaaaa aaggaatgcg acataggtca cacccctttt ttattttctt tgaaacatct
    25141 ttcggtactc gacagcatac aagacaaaag attgtaaaag accgtaagtg atgacggcaa
    25201 aaatgactct cacccacagc gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa
    25261 catagatgac agcagtcgct actttgccat accacttcgc tccacttaat ttgatatttt
    25321 tagatagccc gattaatcca gctacaaaca taaagccttc ttttatcaag aatagaccaa
    25381 acaacagcca aagccacggt cgataccatg ccatagacaa aatcagaaaa agttgtgtca
    25441 atttatctgc aaacggatca agcaccttcc ctaagtctgt tacctgatta tatctccgag
    25501 caatgacccc gtctagataa tcagacaaac ctgagacaac aatgaccgct cctgctatat
    25561 aaaatgactg tctcagcaca aaataaagat aaatcggcag caaaaaaata cggaatatcg
    25621 ataaaagatt cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat ttcattttat
    25681 cgccctacct cctcagcact cttttctttt tattttagct tacacgggga ataaaaccaa
    25741 tgatttcact gtttttattt ttctttacag ccactgatat tatgagctaa tttgagataa
    25801 tcacttgctg ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca tttcttttac
    25861 tagtacttta tgagcttttt ttgatcctcg ctctaggtca cgctgccgtt tacgtgtttt
    25921 ataatcgata aacagactgt ctaaatcgta acctgtcggg tacagatctt ctgctgaacg
    25981 caccagacga acacgctgac gaggcacgtg gattattttt ttgtctaaat atacatcaat
    26041 cgtatcttca cctatatctt cataaactaa cgcttctttt tggtattcat tgacaaatat
    26101 ccgatcacct tttgcaaaga gctggcgttc ttttttattc tccttcctat ttgtaactgc
    26161 tgtcttagga agaaattctt ttttctgtgt ccgataggct ttttccgcca agtaacgctc
    26221 tgcttgatgg atcaatttca tcgacatatc cattttatga gcaatccaca aagcttggct
    26281 ttctcctgtt tctccaaccc gcaactgata tttaggacga agtgcttccc gatcgaaagc
    26341 catagcagcc ggcacaaaat cttcatgatc tccggcgaat ttcttgattt ctccataatg
    26401 ggtcgtggca atgatcaatg ccccttgctc atacatagat tccatgattg cgatcgctag
    26461 tgcagcccct tcatttggct ctgttccact gccaatctca tctaaaagta ccaacgtatt
    26521 cctttttatc tttttcaaaa tagcagcgat gttttgcata tgaccagaaa atgtactcaa
    26581 cgcattttct aaattttggt ggtcaccgac atcgacgaaa atctcatcaa agacagcaag
    26641 ctctgtccct tcttttgccg gaacttgcat accgaacatc gccatcaaag tcaataaacc
    26701 gactgtcttc aacacgactg ttttgcctcc ggcatttgct ccggtgataa tcaagcctcg
    26761 atagtcttta ccgatttcaa catccaatgg taccgcatgc tcaggtaaaa atggatgtct
    26821 gccttgcttg ataatgatat gttccgattt attgattcta ggtgtgcttc cattgatttc
    26881 tcggctaaat tttgcccgag caaagatgat atctaacacc gtgatcgttt cgatcaataa
    26941 atcgatcact tcttcgtttt ctgcgatagc accagtcaat gccgccagga cttgatattc
    27001 ttcactgatt tcttcagctt ttaacaactg atagtgttca ttcaatttag aaatcacagt
    27061 tggttcaata aaaaccgtcg ttcttttatt tgattcatcg ataatcgttc catctatttt
    27121 atttttatag ctggctttga taggaatcgt atagtattcg cctttttgaa caatcattgc
    27181 ttcttggatc atttctttgt ttttcggatg acgcaagaat ttcagtaatt ttgactggat
    27241 ttctttttcg attagttgta attgtttgcg tactttacgc aaattacgtg acgcgtcgtc
    27301 actgacttta ttatttttta ttttctggtc aatcaactct tctacattta tcaaatcagg
    27361 aagatgtttg ctataagcaa acaacaaggg tgtctggtat tgattcttat caaaaaactt
    27421 tgtgatcatc cgactgcttc taagaaaatc cgcatattcg atcaaatcgg ttggctgcag
    27481 aatcaaccct tttttgactt gttctgtcag tgcatcgatc ctcggcaacc ccataaatgg
    27541 aacatgctga ctgctttcta agatcaagcg tgcttctctt gtttcttctt gacgatccaa
    27601 gacagtttgt aggtttgtcg agaccgtcat atcgctgatt cgttttttgc tatagtttcc
    27661 aattgctctt gcttggattt cctttttgat ccgatcaaat tgtaattgac taatcatttc
    27721 gttgtccata ttctctcctt ttcttgaaga gatacctgtc ccaaaataaa taagggccta
    27781 gagataaata aaaggataga acacagctta aaaaagtcat gtcctatcct tattagaata
    27841 caaagatatt gttttcctga caggtaccgt ttcataagca caccaaataa actttgttta
    27901 ttggctattc gacttactga ttagatacca gctacactca caaaacatac cccattcttc
    27961 taggtcttat ttattttcca cttaactata gcaagacaca ttttggttgt caaactttag
    28021 tatgttttat agatttacac cattaatgaa tagacaccta ccgcgataat tgctccgatc
    28081 attggacctg cgattggtac ccatgaatat gcccaatctg aagttccttt attagctatt
    28141 ggcaataccg catgtgctaa tcgaggacca aagtcccgag cagggttgat tgcatatcct
    28201 gttggccctc ctaatgaaag accgatggct aagatcaatc cacctacagc gaatacgttc
    28261 gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc caagaactaa tacaaatgta
    28321 ccaattaatt ctgtgatcac atttgctgga taattccgta tagcaggacc tgttgcaaac
    28381 gttcctaaaa tcgcgctttc atctttcgtt gcattccaat gtggcaaata agaaagccaa
    28441 acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa tgatgaaagg aacgacaaga
    28501 ttccaactaa ttgcccctgt catcgccata gctagagaga ctgcaggatt caaatgagct
    28561 gggcccatga atccagaaat atagacagcc attgttacag cgagtcccca accaaatgcg
    28621 atgacaatcc agccagaagc ctgtgcttta cttttgttta aattcacagc cgcacacaca
    28681 ccatctccaa gcaaaactag gatcagtgtc ccaataaact ctcccatgat ttgagtcata
    28741 tcactgttca tttatttctc cccctttagt tcccgcagat ctgattctgc aatcaatgct
    28801 tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc attcaaaata tccagcaatt
    28861 gcatcgatca caggttgctt gatctcatca agctgatctc gttcaaacaa cagatgattt
    28921 gtacgacgaa tgagataatc gcctggggcc aaaaccattt cttcttctaa accataacgt
    28981 aaccgagctg actcagcgag actcaaacca ggataaggtg ccatttcttt tgctaattca
    29041 aagatacgtc gggcattagt cccgtaaaaa tcagcgatat aagtagcatc ttcttcctct
    29101 aaaccggctg ctactccttc ttttgctaat tctgtcactg tttcttctaa tttagttgga
    29161 tcgaagtttc cacctgaaat ctgatatttt ttagagtcga tttctttcgt ctctattccg
    29221 tattcttctt ttaataattg gcgtatcaat cgcaaagctc cttctgccat cttacggtaa
    29281 tcggtgattt tcccaccaga taaagtcagc aaaccatctg gttctctttc taaagaacta
    29341 cctctggaaa tcgtagaagg tgctttttca tcacggctgt tttccaaatg gttcaacacg
    29401 tcttctactt cagcacgaga aactttgttt tccttatatt cacttactgt atcgacaact
    29461 ttattgaagc tcttgtctga aatcgatcca ttatctccac cattgtaatc agaaccagaa
    29521 ttaccaatca acagtggacg aagtcctgcc cagcttgctt cgatatcatt caatgtgata
    29581 tttgcttctg gatagcgatg attgatcaca tccaatagat aatctacatc ttcttgtgtg
    29641 actttcggat ccgtaaaatc tccttggtaa tccgtatctg tcgtaccaaa gtaagtcttg
    29701 ttttctcttg gaatagcaaa aaccatccgc ccatcttgct ttcctgtatc gaagtatgtg
    29761 ggttgcggta caggcagttt tttcgcatct acaactaaat ggatcccttt agttggacgc
    29821 attttaggag agactggacg cgtaaaattc aagtttcgta ctttatctac ccaaggacca
    29881 ctcgtattga tcactaattt tgctttgatt tcgatcactt catctgtcag caggtcacga
    29941 gctttgacgc caacgatttg atcgccatca tataaaaagc caaccgcttt cattttactt
    30001 actagataag ccccatcttc ggcagccttt ttgatattat caatcactaa acgggcatca
    30061 ttgttgcgga aatctagata gacacccgcg ccttttagcc cttctttttt caaaaacggt
    30121 tctctttcca atacttcttc tggtgtcagc gtataattct catatttggt tcctgtcaca
    30181 tttgccagtt tgtcataaag gtccattgct actttgaccg agaacatatt gaaggttgtt
    30241 gctccttcgc cctcatagat tggcaaaagc attggatctg gttttgggat gtgtggggca
    30301 atactttgta cgactgcacg ttcaccaact gtgtcagcta ctacttccac atcaaatgtc
    30361 ttcagataac gaataccgcc atgaacaagt ttggtcgagc gagaggaagt cccttctgca
    30421 aaatcctgca tttcgatcaa tcctgttttg attcctgatg ctgctgactg gactgccaca
    30481 ccggcaccag tgattccgcc accgatgatc aacagatcca attcttcttg ctgcattttt
    30541 tgaatgctat cttgtcttgt cttgtttgaa aacatatgga tttcctcctt attcaaatgc
    30601 ttgggtagct tttactgctt ttttccaacc gttgtaaagt ttttctcggc gctcttcctc
    30661 catttgaact tcaaatagct tgccagcttc ataaaattcg cggatctcgt ctgtatcttt
    30721 ccaataaccg acagctaatc ctgctaaaaa tgctgcccct agcgccgttg tttccaagtt
    30781 ttctgctcgt tggatcggta cattcaagat atctgtttgg aattgcatca gatattcatt
    30841 gtttgctgca ccgccatcta ctttcaatac aggaatcttg attccagtgt catcttgcat
    30901 cgtatcgatg atatctctca cttgataagc aatcgactgt aaagtagctt taatgatatc
    30961 ttcttttgtc gtaccacgtg tcaagccaaa catagcccct cgtgctgcct gatcccaata
    31021 aggagcacct agcccaacga atgcaggaac aacataaacc tcatcttcgc ttgtagagcg
    31081 tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt tgaagtccat ctctcagcca
    31141 ttgaatcgaa gaaccagcca caaaaatact tccttctaaa gcatagtaaa cctttccatt
    31201 tatgccgtaa ccaattgtcg tcaataaatt atttttggat aattgcggtt tttcaccggt
    31261 attcatgaca ataaatgaac ctgtaccata agtattcttg accatgcctg gttcgaatgc
    31321 catttgtccg aataatgcgg cttgctggtc tcctgccatc cctgaaattg gtgcttcgct
    31381 tccataaaaa tgataacctt gcgtataacc ataaacttct gagttagaag tgacttttgg
    31441 taacatcact cgaggaatat ttagtaaatc taaaatctcc tgatcccaat ctagctgatg
    31501 gatattgaac aacatcgtcc gactggcatt cgagtaatca gtaacatgtg cttgtccatc
    31561 ggtcagtttc catactaacc aagaatcgat cgttccaaac atcaattcgc ctttttcagc
    31621 tcgttcttgc gcgccttcta catgatcaag aatccagcgt atcttcgttg ctgagaaata
    31681 ggcatcgatc actaaccctg ttttttcgtg gaaaaattct tgatagcctt cattttttaa
    31741 gttatctgca attccagctg actgtctgga ttgccaaaca actgcatgat agattggacg
    31801 tcctgtatct ttttcccata ttactgttgt ttcacgctgg ttcgttatgc caatcccagc
    31861 aatctgattt ggtttgatcc ctgattcgat gaacgctccg gcaataactg actgtacaga
    31921 attccagatt tcatttgcat catgttccac ccaactttct tgcgggaaat attgcgtgaa
    31981 ctctttttga gaactgccga tgtgtcttcc tttcttgtca aagataattg ctcgtgagct
    32041 agttgtacct tggtcaatcg ccatgatata cgttgattct gtcattgtga ttccctccat
    32101 tatatgcaaa cgctttcttt aactactctt atcatactac tgttttaaaa aaagaaactg
    32161 tcaaaatttt attctttttt tctttttttg acagtttttt attatgaagc tattttttaa
    32221 tcaattcttt tcttttttca gcgtctctat tcttcttttc aatcgaataa tgtcgtaagg
    32281 tgacgcaaat tctgacagat aatattcaca ctgttttttg tttctctggc aaaatgtgat
    32341 caccaaatca tattctttac cttcttcata acttccaatc tcaataccgc cgattcccct
    32401 cagttctctg atcagatatt gttgaaaagc aatacgataa atcggcagac tttctaaatc
    32461 gattccaatc actactggtt ttgcaatata aaaatcgatc atcaaaagaa cattggcata
    32521 ctggatcatc aggtaactaa gctcactatc ttcagctctt ttcctcggca attgctgcac
    32581 cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa tgcccgagca ttttctgctg
    32641 ctgttccaaa agccgatcac gctcatagac ttcgatcttt ccaacaaaga aaaaccattc
    32701 attgttgatc tgggaaattt ggtagcctac tgccttttct tctttgattt tcaaacgacg
    32761 aggacgataa tgccataaca tcgtttcccg aatgtaagta tctaaaacag cggttggtaa
    32821 ctttttgctc cgtgtcaaat catactgata aaatatttct tcgtctacta tcgaaaaaga
    32881 aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc agttctgctg ctgttcgact
    32941 aagatgcatc acgatgattg gatcgattgc ttgataaagc cggtccgact gatagagtaa
    33001 tttcttttct ttcaaagttg cgtacgtact tttttcattc aatagtcttt ttttcgtaat
    33061 cccgagccag caagcaatct gtgcttctgc tgatgccgta aacgttgttt ctaatacacg
    33121 gtccaatcct agaatgaacg gtcttttttc tggtgtattt tgcaaaaaaa gcggtcgctg
    33181 atcttccggg atatattgga atagttcata ataaaaatat cgtatctgta gctcttctcc
    33241 gtataattga ccgtttttga tttgtaatcc aaattcctct aagagctggt tcaattcttt
    33301 gatctttcga aaaacagaag actcactgat attaaacgct gtcgctaatc gaacgatgga
    33361 tacttgctga tgttctaata aatagacgag catcttaaac ttcaagcttt cctgcaataa
    33421 aaacaataga attttttcca aacttacatc ttcgtccaac acaagcagcg cctgatactc
    33481 atttcgctgt acttctactt ttttttccat cattcggcca atcaattcaa tatcttccaa
    33541 atattgatca aacgaggctt ttgacagttg gacatgctct ctcaaaccat tgatagaggc
    33601 tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc tgtaactgct cttttctttc
    33661 taataatgct tcaatcttca taggcagtat tcgccacctt tgaccacacc atatctggca
    33721 cacggtattc tggctggaaa aatagctcat gttcttctaa ctgttcaaac gtccatcctt
    33781 cttctactgc aattgtcagt tggtcaagaa ggtggaacat ctcttttttg ctttggagct
    33841 gtgctccaag aatctttttt tcagaatcaa atagaaattt gagtttaact ttgctttgat
    33901 ttattgctgt aagcttgtag tctacgtatc ctgtttcaat tttttgggga taaaaaatgc
    33961 tctcttcttc tgtcaaacct acgcttccta agaaatagcc aaaccactga ttgccgactg
    34021 gacgataggt tccctgatcc tttgtctgtg agccactaat tgtcttgctg acatttcgtg
    34081 ctgtacggac agcgttattt actagagaaa catacatttt ttcatttgtt ggacgaaaag
    34141 ttacttggat cgcatcgcca atcgcataaa cgtctttttg agacgtttgc aagtagtcat
    34201 ctacccagat cgttccatca tcattcaatg ttaattgttc ttcccacatc tgattgtcgg
    34261 ggcgagaatg attagccagc aaaacaagat catagttctc ctttcttttt tccgtctcta
    34321 caatagcggt ttcattttcg gtcaaagaca caacttgttc ttctaaaaaa agtgaaagcc
    34381 cttgattttt catttccttt actaacggtt ctgtcatttc aggatctaga taacgaaaaa
    34441 gaattgactt tcggctttcg taaagatgga tttgtttttt tcttgatgcc aacccttcag
    34501 ctatttcgat tcctgcttgt cctgcaccaa taattgcaat tttctttgca ttcatgatct
    34561 tttgctgaag agccgttacg ttttgtatct ttttaactgg gtgaataagc gttgattctg
    34621 cagaaatatt tcgaaacttt tgatttgaac cagtcgctag aatcaaacga tcaaaatcca
    34681 atttttttcc tcctgctaaa acgacttttt tttctttcaa tcctattact gtcttttctg
    34741 tgtaaacatg gattcgatag cggtcagtca gttcttcttt cgtgatccaa gaaagttgtt
    34801 tttcactggt aacctttcct tttaatatca aattgaaccc gctgggaatc catcctaact
    34861 tcgattgctt ttcaatcaaa tatatttcac ttgttggcat caattggcga agataaatag
    34921 ccgcatgaat ccctgcaaac gacccgccga caattacaat gttcatctgc attcctcttt
    34981 cttataaggt gttctactgc taacttctca atcagaaaac acagtattca taactcgctt
    35041 tcaaccttat gatacatagc gatgcagcag aatcaaccct ctccatcata ccgtgttcta
    35101 ggtctgcttc tcaataatgt actagtggac ctaattgaag agtatcattt ctgaagagat
    35161 gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc ttttacgtac ctctgtccaa
    35221 ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg tgtaatgtaa tagttagaat
    35281 agacacatca tttattttta attctttaat gcttcaacaa ctgcttcgcc taattgatga
    35341 gcagattgag gattttgccc tgtaagaagc cgcccatcaa tttgaaccac ttctgaaaaa
    35401 gctgctccag actgatagtt tgctcctttt tctttcaaag catcttctgc taagaatggt
    35461 acttcattcg ttgtaccatt tgcttcctct tcttcattac tgaacccagt cactgtacgt
    35521 ccagctaaaa tagattttcc agactcatct gtaactggca acaatcctac aacaccgtga
    35581 caaacagctg agaccagccc ttcatttcga tagatttgtg aagcgacttt cccaagatct
    35641 attgcttctg gaaaatccca cattgttccg tggccaccag cataataaac cgctgcataa
    35701 tcatttgcat ccacatcatc aggacgtaag gtatttccca aggcccgatt tctgaaatct
    35761 tcatcttcat aataggccca atcgatttca tccatattca aaacactttg cggttctaaa
    35821 ggtacatatc cgcctttagg actgacaaaa tctgcatcga tcccattttt tactaataca
    35881 tcataaaaat gagtcagttc acttaaccac aaacctgtag gacgctcgat cttttcgtac
    35941 ttttctacat ttgttaatac gatcaatact ttcattttcc catcctcttt ctataaaaat
    36001 tgcgttattg ataacgtgct ttcaagaaat tatataatgg gcttggcaaa taaagctctt
    36061 tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc acggttttcc aataatttct
    36121 ctgcccagtc aggatcgacg atcatttgct gtccaattgc tgcaagttct gcatatttca
    36181 atacctgctc tacatcttcg cgattgcgga tcccaccgac accaactaaa gggatctttc
    36241 cttgaatcgt gttatggacc acttcaagaa tcgatttatc gttgtattta tcagaacgag
    36301 ctattcgatc atacatattt aatgaaacat gcagataatc tagttttgac tcacgtaatc
    36361 gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg tgtttcatat tcttctggtg
    36421 agaaacgata ccctacgatg aatggttttt tagcatattg ttcaaccgct gagaaaaccg
    36481 ttgttaataa ttcttcaata aaacgataac gattttccaa gctaccgccc cattgatcct
    36541 ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa atacgtattt gctccatgca
    36601 actcgattcc atcaaatcct gattcaatcg ctctacgtgt ggctttgcca aatgcttgga
    36661 ttgtttcaac gatttcttct tcactcattg ctcgtggtgt ttccgcatca ggacgttttg
    36721 ctgcaactgc acttgcagat acaggctgtt ctccagataa agttgctcga cttgtcattc
    36781 gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc tccttggatt gctttcgcta
    36841 gctcacttaa tcgaggaatc atttcatcat gagcgatgct gagttcacct ggccagcctt
    36901 ttccgatatc ttggacattt gcagcaccag taataacagc accaagacct ttagaacgtt
    36961 gtttataata agtgatctca tcttcggtga tcactccatt gaaaaagctg agttgtgtcg
    37021 tcataggcga cagcatcaat cggttcttca attgaactcc tgaaggtaaa gtaattgatt
    37081 gatcaaattg tgacattgtc attcctccca taattcgctc tgattaacct atcgagaatc
    37141 atctatcatt aaaccactcg attagttgta catgcaacta atttgcttaa aaaattttat
    37201 tttttttgta acttatctgt tgcatcattc attgtctggg ctaattcttt acttgcttca
    37261 tttcccaaaa ctgctttgta tgactgatga agttcttccc agctaccatc gacttcctta
    37321 gcaaattggc gtccttgttc tgtaagataa actaatgaca agcgaccttc gctaactgtt
    37381 ttgacgtatc ctttatattc taatttttca acaaaacgag caatcgttga tggggcggaa
    37441 tagagcattt gtgcaatatc tttttgtggt attccatctt tttcttttaa tatcaataaa
    37501 ataaaaccat aagttggtgc cagacctgtc cttctaaacg cttcatcagc taatttattc
    37561 aacatgcgat ctagcttttt cacgctaaag aaaagacatt catctaacgc tttttcaatt
    37621 ggcttttcca tttcatcccc tccattggcg tacatatcat agtatgattt ttattttttg
    37681 tcaaaaaaca cggaataatg tccgcctcct ctgtctagat ttgaaaaaaa gaagtagcca
    37741 tcttcaaaga atttcggctt gttttcgaca aaactccttc tggaacaccg tgaatcagag
    37801 gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat tttgtgaaaa tggctttcca
    37861 catttttgca taaatttggc ttaataccga aaaactggct tttgaacacc gtttatatca
    37921 gaggttgtga aaaagccgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc
    37981 tcatattttg tcgttttttg agctaatgcc gatggtcagc tgattgtaca ccgtttattg
    38041 agaggtcgtg agcctgccgt tgaactccga gtcacaagga gcaatttcaa aaaacagctt
    38101 ctcatgtttt atgactatgg tgacttgtga tcgaggagtt ggcaggagaa tactgtttat
    38161 tcgtaaagaa ggggctgtga caagactttt gtcacagtcc cttatttcct tttattatag
    38221 gatttcgcca tttgattgga tgacttgtct gtaccaatca aatgattttt tctttaaccg
    38281 tttctttgtg ccattccctt cgtcatcttg atcaacatag atatatccat aacgttttga
    38341 catttgtgaa gtagaagcgc tgacgatatc aatgcagccc cacgacgtat aaccaaataa
    38401 atctactcca tctttgatag cttccttcat ctgctcaata tgctgtttca aatagtcaat
    38461 gcggtaatcg tcttgaacgg ttccatcttc ttctactttg tctatagctc cgagtccgtt
    38521 ttcagctatg aacagtggtt tttgatacct atcatataat tgattcaaag caattctcaa
    38581 acccttagga tcaatctgcc agccccattc acttgatgga agatacggat ttttaattcc
    38641 tccagttatt aagtttcctt ggaccccttc ccaattttct ggatgagcac tgactgcact
    38701 agacatgtag tagctaaaag aaacaaaatc aacagggtag gctgctaaga tttcttcatc
    38761 acctacttct atttttaagt tgacaccttt ttcttcaaac attcgttgcg tatacgctgg
    38821 ataatatcct cttgcttgaa catcactaaa gaacaaagaa gttctttgct gctcaattgt
    38881 tttttgaata tcatccggat cacaagaata cggaacaaga agctgatagc tgatcataca
    38941 accgacttgt gaacctttga taatttcatg acaagctttt gtagctaaag cacttgctat
    39001 aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt tttcttcaat
    39061 tactcctacg ctaacaaaag ggtgatgttt tacacagttg atctcattga aagtcatcca
    39121 atattttact ttatttttat agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg
    39181 aataagcttt cgattaggcc atccaccata ttctgtaacc aaatataacg gcatttcata
    39241 atgggaaaga gaaacgactg gctcaatccc atattttaga cattcgtcaa acacttgatc
    39301 ataaaaaaat aatcccgctt cattgggtgt ttcttcatca ccatgaggaa aaattcttgt
    39361 ccatgcgata gacatccgat agcatttaaa acccatttca gcaaataaac ggatgtcttc
    39421 tttaaagtga tgatagaaat catttccgtg tcttttagga taataatact tgctgttggg
    39481 atcctgcgcc gcttttactt gctgatgcgt catcatatgc cattgatctg tccatttttc
    39541 tttaggtaaa tcgctgtcga atgtatacac atcagacaca gataatcctt tttcatctac
    39601 atcccatgca ccttcacatt gattggccgc tgtcgctccg ccccataaaa atccttctgg
    39661 aaattttttc atatcgatcc ccctaaactt gctagttttc ttgtttgatt atttttctta
    39721 tgactagtaa tgatattttt gaatctacta ctatttgtaa caataaccat tgtggtcgga
    39781 tcatatccct ctttgattaa ttcttgcaaa tctacttcca tcaataaatc gcctttacgt
    39841 actttttttc catcagatac tgcactttga aagtatttgc catttaaatt cactgtatca
    39901 ataccgatat ggatcagtat ctctgctcct tcttcactga ctaccccaat tgcatgtttc
    39961 gttggaaaaa ctgttgaaac ttccccgttt acaggactgt agatttttcc gtccgttgga
    40021 atgatcgcaa acccttctcc taaagatcca ctagcaaatg ttggatcgtt tacttcagat
    40081 aaatcaatta tttcacctgc tgcaggagta cttaacacta tatcctttga ggattcttct
    40141 tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac aggaactgat catcgctaag
    40201 aaataactag caactagcca aacaaatcct tcaccaatat aaatcgggaa agacaaaata
    40261 cttcttgtag caactgccaa agcaccggct cctgcggcac cagctactcc accaaatata
    40321 gctccacata tacaagcaat gtaaaatggt cgtttatatt taagagtcac accataaaca
    40381 gcaggttcag tgattccaaa taatgctgtg ataaatgcag ataaagataa actttttact
    40441 ttcttatttt tcgttttcaa aaatacccca agtactgctc ctgcttgcgc agctacggca
    40501 gtcatacatg ctggacctag cgtatcacga ccgtagaaag aaagattgtt ggtcattact
    40561 ggtacaagtc cccaatgtaa cccaaaaatc actaacactt gccaaaatcc gccgataaca
    40621 gcaccagata aaacagggct aaagttataa acagctaaat atccagctgc aatcagctga
    40681 cttgcataat ctgcaattgg acctacaact aataaagtaa ttgggaccat cacgatgata
    40741 caaatgaatg gtgtcaataa atttcgaata ctttcgtgca agcttttgcg aatcttaggc
    40801 tcaatataag aaagaaccca tacagctaaa attgctggaa taaccgtact tgtatacctt
    40861 gctagaatca cgggcatccc tagaaatcgc aaggttatgc tatcactata tgcactgaca
    40921 atcgtaggat aaacgagagc tgctgcaata accatagcga tgaaacgatc tgtattgaac
    40981 ttttttgcag cagtatatgc caaaaatata ggcaagaaat agaaaaatgc atcagctgct
    41041 gcattcaaaa tgatataagt tcctgattca gtagttaata aattcaaact cgtacataag
    41101 gcgagtactc ctttgatcaa accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca
    41161 gcggcgatca tgtctataaa agagtttaaa atgccttttt tctcagttcc tctctttcca
    41221 ctagatgcat catcttctag tttcatttgt ttggataata cgtcatacac ttcatttact
    41281 gcatttccta ctacgacttg aaacattcct ccagcttcga ctacagtgat cacaccttct
    41341 agttgctcta ccttctcctt atctgccttc ttattatctt ttagggtaaa gcgtaacctt
    41401 gttgcacaat ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg gacaatatct
    41461 ttcgctaatt gtgaatagtc catttttact tcccccttgt taattaatgc cgtagcaatc
    41521 cttcttacaa gataagtata gttcttagaa aacggtttct ccattcaatt agtacccctt
    41581 ataatagcga acgaaactgt acaatagtgt gtatggttta cttagccgag caaatcactc
    41641 actgcttgaa agaaaacaag tatatcgttt ctcttatagt caaaggagac aaaaaaaaca
    41701 cgcttgatct tcctttgctg atgtataagt ggtatctcaa gctgatactc tgtgattttt
    41761 gtttctactt ggcttttgat aagacagcac aacaaagtct cggcatttaa cggaagcata
    41821 actaggtcag aactaggaga gattagttgc tgaatggaat ctatcttttt atggattttt
    41881 atttgatttt cttctttgta gtaatgatca agcagttttg ttttcttttt ttcttcaact
    41941 atggtcagct tttcacgcaa aatctgataa atctcccaag attcaaactc atctagaaca
    42001 ctaggtagat aaatcaaatc atcccactca aaaataagct cttgctcagt agtcaaaatt
    42061 gcatcatatt ccgttatttt gtttgattga tttttgaaaa gatacactgg ttctatccgg
    42121 cattctttga tccacatgtt catactttgt tgaatctgtt tttttagtgt attcaaaata
    42181 ctaattggct ggttactaac aatcagtatc tccccttgtt ttcgaaaagg attcaacgct
    42241 tcggcaagat acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt
    42301 attgggaaga aacgccgaat taaataaatt tctaaaggat aacgggagat gatcttttgt
    42361 acaaaatgat tcatggtatt atgtccggca tccatcctcg ttttcatttg tttgatatgg
    42421 gttagcaaaa attctgtttc atggaacaat aacgaaacag gaagatctaa aaaacgaata
    42481 atcgtttgct caaatgctct tagattcttc tctatttctt tgtttttttc atcattaatc
    42541 atcaaataat ttaactctaa tagtcgattt tcaattgctt gtttttcttg attggaaaaa
    42601 gaatagggta gttctgatga taactttttt gtgagggtct ctaccatcgg aataaataat
    42661 gtatacctgt ttgttttttc taaaagttct tctcttcgaa gaatggtcaa aacgagatac
    42721 cgagaaaatc gtatgaagtc ctctcctgaa acaatataat tatatgatat aagaacctct
    42781 tttaatgtct tttggacagt cggcatgata ttggaaaaaa ttgtactgat ttgttgaggc
    42841 aaccttgcgt gttggatcac cgtttctgtt ccaacaagag ataagaaaat tctttttgaa
    42901 ttttcttcag caatgatttt tagtcctttt gtcgacgaaa cttccagttc taatttagga
    42961 ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg atacggctgt ttttgaaaga
    43021 aagaaaatgt ctgctatctt ttgcatactg acataccctt tttgaaacaa taagtacgtc
    43081 acaatagttg aagctcggaa atccatgctg gaatagattt tatgtttcgt cacctcttgt
    43141 atgatttgct gtttagtttc agattcaatt gattctaaaa aataacctga acgtttattt
    43201 gactggataa tgattttctt ctcgtagata gcaagcatat ttttgatttc agtttggatc
    43261 gttttcttgt tggtacctaa agcttcagct aattcttcgc ttgttatcca atgattctta
    43321 tttcttaata ctacaagaat ttggagttgt cgtttattta agtccatttg cttacacctc
    43381 ctctttgtta taaacataca ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt
    43441 taaaaatcat ttgaagaaag atctatattc aaggttttta ccattagact ggataatttg
    43501 tttataatat tcaaaactat cttttttcaa tctcctaaag cttccgctcc catcattttg
    43561 tctatccaca taaataaagc catatcgctt cctcatttcc cctgttcctg ccgatacgat
    43621 atcgattggt ccccaccata agtaaccaat gatttggcaa ccatctttta ttgcctcagc
    43681 aatttcctga agatgtattt ttagatactc acaacgtttt ggatcgtgga ttcgattcaa
    43741 ttcatctggt gcttcatcta gtccaatgcc attttcaact atgaataatg gtaattcata
    43801 acggtctgtc aattcattca tgacataacg aaaccctaga ggatcaattg gccaactcca
    43861 aggttcggga gatttttcct tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc
    43921 tacgcgcatt tctgcttctt ttttatagac agcgctacga taataactaa atgcaatata
    43981 atctgccgtg ttagcttgca agcactgttc gtctccatct aacattactg gtgcacagtc
    44041 ttcttccttc catgtcctgt aaatataagc tggataatgc cctcggcaca tgacatcgga
    44101 gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat acattctctg gatcacagtc
    44161 atatgcataa gtcgctaatg cagaaaagct acacataata cccatcttag catttggatc
    44221 gattttcttt aataattgag tagcacgggc gttcgctaga aacatatgat gacaggcttg
    44281 ataaatcact ttttttgtta gtgtagcaat gggttcaagt ttatctctag cagggaatgt
    44341 aaccaatcca gcagctgcat aaggcatacg aaaagcatta ttgatctcat taaaggtcat
    44401 ccaatatttc actttccctt ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa
    44461 gtcgattaat tttcgatttt gccacccccc atactctcct accaatgcta agggggtttc
    44521 ataatgacta agagtaatta ctggctcgat atcatatcga tttaatgtat tgattagtct
    44581 gtcataaaat acgagaccag cctcatttgg cttctgttcg tctcctcttg gaaatattcg
    44641 tgcccatgag atgcttgttc gaaaagcttt taaacctaac tccttcagta aacgaatatc
    44701 ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa agataatgga gtgattcgtc
    44761 taaagcaatc tcccaaatct ttttttcttc attccactga atactaggat gctggttcat
    44821 gatcccgata agcgtatctg tcacattagg taatttgccg tcttccaacc atgcaccttc
    44881 acattgatta gcagctactg ctccgcccca taaaaaattt tctggaaata tttcctgatc
    44941 cataactatc tctcctcgct atcaacaatt tcttacttga agtatagctc gattcatcaa
    45001 aacaagatat tctaactatt tcggcagaag agaagaaaaa aattgaatgc tactcttcat
    45061 ttttaatatt tatatataac aaatacataa gacgatcccc gaaagaaaac actcgttaac
    45121 catctaagca aacgatttat tttacttcca acacaaatgc ttcataaggt cgtaaatcaa
    45181 ctttgccgtc tggtaactgt ttcagaggat aatttgacac aataaccctt aaagcttcac
    45241 gtggaacagt gaacgtctgc ttttcactgg acatattaca aataacgagc cattgctggt
    45301 cttcatatat gcgagaatag gcaaagacat gctcaggcgt cttttccact aattcatagt
    45361 ctccccagac gatgatgtca tgttttctac gcaaatcgat caatttcttg tacatataaa
    45421 aaacagaatc ttgatctgct aacgcttctt ctgcattgat ttctttataa tttggattga
    45481 ctcgaagcca tggaattcca gtagtaaacc cagcatgttc acgggcgttc cattgcatag
    45541 gcgtcctagc attgtctctt cctttcgcat tgattgattc caagatttcc tcttttgtaa
    45601 atccagaaga aatcctttcg ttatacatat tgattgtctc aatatctttt gcttccgcga
    45661 tatcatcgat tgggcaattc gtcatgccta actcttctcc ttgatagata taaggggtgc
    45721 ctttcatcat atggagcaag atagcaaaca tcttggcact ttcttttcga tacacatcgt
    45781 catttcccca acgagaaaca attcgcggaa gatcgtgatt attccaaaat aaactattcc
    45841 atccttcatc acctaaagat gtttgccatt tggataatac ttttttcagt tcagagatac
    45901 tcaagggttt caaatcccat ttgtctttgc cttcttgctg atctaatcct atgtgttcga
    45961 attggaagac catggataat tcatttcttt taggattgga ataaagttta gcgatttcgg
    46021 tagttgctcc ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa gttgcttgat
    46081 tcatttcctg aagatactca tgcagtttag gtccattact tgtgatttct ttatccggtt
    46141 ctttgccaat catatcgatc acgtccatac gaaagccccc aataccttta tcgatccaga
    46201 aattcatcat ttgataaatc tcttggcgaa cctgtttatt tttccaattc aaatcaggtt
    46261 gacgcttgct atacaagtgc agaaaatatt gccccgtttt ttcatccaac tgccaagcag
    46321 aaccactgaa tattgagcgc aatctattag gctcttttcc attgaccgga tcacgccaaa
    46381 tataatagtc ccgataagga ttttctttat ttttacgggc ctcaatgaac cacggatgtt
    46441 catcagaagt atgattcacc accagatcca tgatgatacg gatatttctt ttctttgctt
    46501 cttcaatcag ttcttccatt tcttccatcg taccgaattc tgccatgatt gcttgataat
    46561 tactgatatc atacccattg tcatcattcg gagactcgta tactggagaa agccaaattg
    46621 catcaatacc taaatacgcc agataatcca acctggaaat aatcccccgc aaatctccaa
    46681 taccatcttg atttgaatct tgaaaacttc ttggatagat ttgataaaca actgaatggt
    46741 gccaccattt ttgttccatg attttcctcc tataaatgga tgtttctatt ttttgcttaa
    46801 tcatttattt tagtctctct cactaaaaat tgtaacgctt tcaaatagct attgcacctt
    46861 gttatcggta acagaataaa acaaaaaaac agctgaggaa attccctcaa ctgttttttg
    46921 aaaggattga tttatgcagg gaagaatgct gatttcacaa cttctttata aacggtgttc
    46981 actcagctgt cccttggtat tagctcaaaa aacggcaaaa tatgaggaac tattttttcg
    47041 tttttttctc taatactcag gccagaacac tgatttcaca acctctttat aaacggtgtt
    47101 cactcagctg tccctcggta ttagctcaaa aaacggcaaa atataagaaa ctattttttc
    47161 gtttttttct ctaatactca ggccagaacg ctgatttcac aacctcttta tttcacattc
    47221 aatatccaca tgaatcccac aaatacaaag aacagaccat acatgattgg agatacttct
    47281 tttcctcttt tagctgctaa catggtaatt ggatagaaga taaaacctaa tgcaatccca
    47341 tctgaaatac tataagtcaa aggcatgccg atcaagatca agaatgaagg gatcgcaatc
    47401 tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc taccataatc
    47461 aatgctggtg cagttacttg tgaagtaaca actgatagta atggagaaaa gaacaagcct
    47521 aacaagaaga aaatccctgt agtaatagct gtgatacctg aacgtcctcc aacggcgata
    47581 ccagctgatg attctacaaa tgcaccaaca ggagaagtac caaataagga acccgcaagc
    47641 atagctgttg aatcagaagc taaagctttc cctacacgag gcatcttgtt gtctttcatg
    47701 aaacctgctt ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa agtcacaagc
    47761 aagaatgtca gcactacgac ccacatttga agagaattga tgtcccctac atgttttaat
    47821 gcaactaaaa atgttggttt caaacttggt gctgcggcaa caatttcaga aggcattgga
    47881 atcaaacctg tgaccagacc taaaatcgtt gtagctgcca tcccgatgaa gatgccacct
    47941 ggtacacgcc gaacaagcaa gatagcagtt acgactaatc caaaaatcgt caaccaagta
    48001 gaaccaacgt tgagtgatcc taatcctact aatgtagatt cattcgaaac gatgatccca
    48061 ccttcgctca gaccaaggaa agcgataaat agaccgatac cgccagaaat cgcatatttc
    48121 aaatctgctg ggatcgcatc aatgatcatt tcccgcagct tgaagattgt gatcaaaata
    48181 aaaatcaaag aagcaacgaa tacgccggct aatgctgttt cccatggaat ccccattcct
    48241 aaacaaacag aataagcaaa aaaagcattg atcccaagag caggtgccgt tccaattgga
    48301 taacgggcta atacccccat taaaacacag ccgatcgcac tggctaatgc agtcgcagta
    48361 aacactgctc cttcatccat tccagaagca cctaatacag ttggatttac aaataagata
    48421 taagccattg agataaatgt tgtaaatcct gccagtacct cacgttttac cgtggtgttc
    48481 aatttttcta tttcgaagta ggaaagtatt ttttccttca tcttccatcc ccctaaatcc
    48541 gaacattata aataaagtcc gttaaaattt cgatttatta ataaaacaac gtgttttatc
    48601 atatactaaa acaaaacata agtaaacaaa aaattcggat tttaagcaaa atccgaacgt
    48661 tccatctgat taaatccctt tatctcaaag ttcatgaaca ttcactttgg attacaagaa
    48721 tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat agtgtgattt
    48781 gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga caggattcag
    48841 attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg tcttcttttt
    48901 tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa
    48961 ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt caatcagacg
    49021 actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg aaagtcaaga
    49081 tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata gtttcaaatt
    49141 catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa aacttctgca
    49201 caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt acaatactct
    49261 taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt tctacaaaga
    49321 atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt tgtatcatca
    49381 tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc attatatcac
    49441 gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat agtttctagg
    49501 attgctttcc tccacaaact ttttttacaa aaaaaaaaac ggcaatgaat gccgctcttc
    49561 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
    49621 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
    49681 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
    49741 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
    49801 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
    49861 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
    49921 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
    49981 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
    50041 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
    50101 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
    50161 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
    50221 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
    50281 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
    50341 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
    50401 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
    50461 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
    50521 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
    50581 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
    50641 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
    50701 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
    50761 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
    50821 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
    50881 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
    50941 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
    51001 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
    51061 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
    51121 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
    51181 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
    51241 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
    51301 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
    51361 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
    51421 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
    51481 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
    51541 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
    51601 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
    51661 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
    51721 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
    51781 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
    51841 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
    51901 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
    51961 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
    52021 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
    52081 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
    52141 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
    52201 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
    52261 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
    52321 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
    52381 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
    52441 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
    52501 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
    52561 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
    52621 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
    52681 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
    52741 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
    52801 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
    52861 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
    52921 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
    52981 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
    53041 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
    53101 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
    53161 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
    53221 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
    53281 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
    53341 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
    53401 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
    53461 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
    53521 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
    53581 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
    53641 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
    53701 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
    53761 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
    53821 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
    53881 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
    53941 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
    54001 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
    54061 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
    54121 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
    54181 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
    54241 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
    54301 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
    54361 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
    54421 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
    54481 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
    54541 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
    54601 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
    54661 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
    54721 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
    54781 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
    54841 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
    54901 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
    54961 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
    55021 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
    55081 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
    55141 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
    55201 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
    55261 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
    55321 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
    55381 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
    55441 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
    55501 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
    55561 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
    55621 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
    55681 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
    55741 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
    55801 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
    55861 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
    55921 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
    55981 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
    56041 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
    56101 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
    56161 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
    56221 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
    56281 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
    56341 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
    56401 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
    56461 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
    56521 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
    56581 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
    56641 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
    56701 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
    56761 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
    56821 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
    56881 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
    56941 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
    57001 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
    57061 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
    57121 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
    57181 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
    57241 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
    57301 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
    57361 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
    57421 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
    57481 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
    57541 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
    57601 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
    57661 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
    57721 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
    57781 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
    57841 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
    57901 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
    57961 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
    58021 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
    58081 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
    58141 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
    58201 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
    58261 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
    58321 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
    58381 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
    58441 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
    58501 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
    58561 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
    58621 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
    58681 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
    58741 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
    58801 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
    58861 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
    58921 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
    58981 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
    59041 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
    59101 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
    59161 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
    59221 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
    59281 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
    59341 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
    59401 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
    59461 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
    59521 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
    59581 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
    59641 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
    59701 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
    59761 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
    59821 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
    59881 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
    59941 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
    60001 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
    60061 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
    60121 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
    60181 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
    60241 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
    60301 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
    60361 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
    60421 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
    60481 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
    60541 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
    60601 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
    60661 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
    60721 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
    60781 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
    60841 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
    60901 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
    60961 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
    61021 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
    61081 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
    61141 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
    61201 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
    61261 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
    61321 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
    61381 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
    61441 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
    61501 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
    61561 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
    61621 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
    61681 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
    61741 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
    61801 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
    61861 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
    61921 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
    61981 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
    62041 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
    62101 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
    62161 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
    62221 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
    62281 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
    62341 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
    62401 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
    62461 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
    62521 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
    62581 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
    62641 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
    62701 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
    62761 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
    62821 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
    62881 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
    62941 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
    63001 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
    63061 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
    63121 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
    63181 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
    63241 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
    63301 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
    63361 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
    63421 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
    63481 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
    63541 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
    63601 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
    63661 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgagtggat caatcaattc
    63721 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
    63781 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
    63841 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
    63901 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
    63961 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
    64021 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
    64081 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
    64141 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
    64201 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
    64261 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
    64321 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
    64381 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
    64441 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
    64501 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
    64561 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
    64621 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
    64681 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
    64741 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
    64801 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
    64861 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
    64921 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
    64981 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
    65041 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
    65101 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
    65161 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
    65221 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
    65281 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
    65341 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
    65401 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
    65461 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
    65521 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
    65581 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
    65641 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
    65701 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
    65761 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
    65821 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
    65881 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
    65941 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
    66001 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
    66061 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
    66121 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
    66181 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
    66241 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
    66301 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
    66361 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
    66421 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
    66481 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
    66541 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
    66601 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
    66661 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
    66721 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
    66781 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
    66841 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
    66901 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
    66961 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
    67021 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
    67081 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
    67141 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
    67201 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
    67261 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
    67321 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
    67381 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
    67441 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
    67501 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
    67561 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
    67621 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
    67681 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
    67741 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
    67801 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    67861 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
    67921 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
    67981 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
    68041 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
    68101 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
    68161 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
    68221 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
    68281 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
    68341 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
    68401 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
    68461 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
    68521 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
    68581 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
    68641 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
    68701 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
    68761 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
    68821 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
    68881 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
    68941 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
    69001 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
    69061 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
    69121 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
    69181 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
    69241 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
    69301 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
    69361 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
    69421 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
    69481 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
    69541 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
    69601 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
    69661 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
    69721 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
    69781 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
    69841 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
    69901 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
    69961 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
    70021 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
    70081 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
    70141 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
    70201 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
    70261 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
    70321 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
    70381 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
    70441 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
    70501 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaacgataa agtgattgtc
    70561 gttaccatat tgacccataa aatctgtact ggggacaaag gtagcgggcg attcagcata
    70621 agtgcccaaa tcaagatcaa tccttgagct aatgcagttg gcaagaagaa attgatggtt
    70681 ttttgcaaat tatccaaaat ccgccggccc tcttttacgg cttttgcaat cgtatggaaa
    70741 ttatcatctc ctaaaaccat atcagctgcc tgtttgctta cttcactacc tttgattccc
    70801 atagcaattc ctacatcgga tcttttcaaa gcaggtgcat catttacgcc gtctccggtc
    70861 attccgacga tttctccatt tttctgcaaa gctttaacaa ttcgcagttt atgttctggt
    70921 gtcgttcgag caaagacatc gactattggc acttgctgaa tcaactcttc atcagacata
    70981 agatccaaat cgatcccttc taaaactttg gcagtatgtt ttaagccaat ttgttccgca
    71041 atcgcttttg ctgtgtcttt gtgatcccct gtaatcattt tcactgagat ccctgcttgt
    71101 tggcattcct ttacagcttt gactgcactt tcttttggcg gatcgatcaa tccggcgatc
    71161 cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt cctgtttcga atctactttt
    71221 ttataagcaa atcctaaaac acgctgacct tttctcgcaa attctgcagc tgtctgactc
    71281 catgcttctt gttctgcaga agaaagagtt gatttagcaa ataatacttc tggtgcacct
    71341 ttgacgtaaa tcaccgctcc tttttcctcc gcatgcatcg ttgccatata tttgtattca
    71401 gaactgaacg gaatctttgc ttctattttt ttcaaaggta gattggctgt ttctgtgtgt
    71461 cgaatcagcg caacttctgt cggattgcca cttaaatttt ctgttttctg ttcttctttt
    71521 gtttttaatt cctgacagtt tttcataatg gacaacacgc aggcttcttc ttttgcagct
    71581 acatccacaa ctgtcatttc attttttgtc aatgttcctg tcttatctga acagatcact
    71641 gtcatagaac ctaatgtttc taccgaagga agtcctttga tgatcgcctt ttcttttgcc
    71701 atttcatgta ctccaacgga tagaatcatg gtcaaaacag ctggcaatcc ttcgggtacc
    71761 attgacacca ccaaagcaat cgtagccgaa agaaggatat gccaatccat accatatcga
    71821 aatgtcgtaa aaataactaa gaataggctc aaacaaagaa tgccttgaaa aatttgtttg
    71881 tttaatcggt tgattttttt aatcagaggc gtcgtttgtt gttcgaccga ttgcaatgct
    71941 tgattgattt taccgatttc tgtcgtgttt cctgtttcta caacgacacc gactgcagaa
    72001 ccagattgta caagcgttcc tgaaaaagcc atatttttct ggtcgccgat cgattcttct
    72061 tctgtcaaaa cggcatgact tttttcagat gcttctgatt ctcccgtcaa aatcgattct
    72121 tccactaata aatcatgtgc ttcgattaat cggatatctg ctggaacgac atctccggca
    72181 cgtaagacaa tgacatctcc ttttaccagt tctttcgcag ggattttttc ttgcatcccg
    72241 tttgatagta ctatcgcttc ttgtcccatc agctgcttca aaccattcaa tgattcttcc
    72301 gcttttcttt cttgccagta actaacgagt ccattgacga tcactacgaa taaaataata
    72361 cttccttcaa tatattcact agaagcaaat ttcaaaaaag aggcaatgat caacacgatc
    72421 atcagcagat cagtaaaatg ctttgctatt tttttccatc gttttgtctg ctgccttgct
    72481 tttatttcat tatatccttg atcagtcagg cgtttctgtc gttcttcttg cgacaagcct
    72541 tctgttgatg tttgatagtt tttcaataca tcttctaccg tcaatctgta ccagttcatc
    72601 ctatcccctc ctgtttaatt gatactgtta ttatacgcag ggaagttttt tcaggcgtat
    72661 cgattatttc aggggggtct gaaataattc gttttgcaaa atgtatctgg gcctaactga
    72721 tatttctatt gcttattctt ttatatacaa aatttaag
//
LOCUS       DASOUD010000004        62812 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.004, whole genome shotgun sequence.
ACCESSION   DASOUD010000004 DASOUD010000000
VERSION     DASOUD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.004"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(160..444)
                     /locus_tag="U4A29_000241"
     CDS             complement(160..444)
                     /locus_tag="U4A29_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679395.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="U4A29_000242"
     CDS             complement(462..1352)
                     /locus_tag="U4A29_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEO0679396.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="U4A29_000243"
     CDS             complement(1372..1590)
                     /locus_tag="U4A29_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO0679397.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="U4A29_000244"
     CDS             complement(1560..3260)
                     /locus_tag="U4A29_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEO0679398.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="U4A29_000245"
     CDS             3527..4300
                     /locus_tag="U4A29_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679399.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="U4A29_000246"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="U4A29_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO0679400.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="U4A29_000247"
     CDS             complement(5124..6095)
                     /locus_tag="U4A29_000247"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO0679401.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="U4A29_000248"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="U4A29_000248"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO0679402.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="U4A29_000249"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="U4A29_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO0679403.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="U4A29_000250"
     CDS             complement(9050..9874)
                     /locus_tag="U4A29_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO0679404.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="U4A29_000251"
     CDS             complement(9847..10527)
                     /locus_tag="U4A29_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO0679405.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="U4A29_000252"
     CDS             complement(10550..11488)
                     /locus_tag="U4A29_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO0679406.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12596)
                     /locus_tag="U4A29_000253"
     CDS             complement(11733..12596)
                     /locus_tag="U4A29_000253"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO0679407.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="U4A29_000254"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="U4A29_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO0679408.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..16258
                     /gene="proC"
                     /locus_tag="U4A29_000255"
     CDS             15452..16258
                     /gene="proC"
                     /locus_tag="U4A29_000255"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO0679409.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16337..17893)
                     /gene="rny"
                     /locus_tag="U4A29_000256"
     CDS             complement(16337..17893)
                     /gene="rny"
                     /locus_tag="U4A29_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO0679410.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18304..19353)
                     /gene="recA"
                     /locus_tag="U4A29_000257"
     CDS             complement(18304..19353)
                     /gene="recA"
                     /locus_tag="U4A29_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO0679411.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19462..20703)
                     /locus_tag="U4A29_000258"
     CDS             complement(19462..20703)
                     /locus_tag="U4A29_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO0679412.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="U4A29_000259"
     CDS             complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="U4A29_000259"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO0679413.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21730..22653)
                     /locus_tag="U4A29_000260"
     CDS             complement(21730..22653)
                     /locus_tag="U4A29_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0679414.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="U4A29_000261"
     CDS             complement(23392..24678)
                     /locus_tag="U4A29_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO0679415.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="U4A29_000262"
     CDS             complement(24671..25939)
                     /locus_tag="U4A29_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO0679416.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="U4A29_000263"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="U4A29_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO0679417.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="U4A29_000264"
     CDS             complement(26711..27157)
                     /locus_tag="U4A29_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0679418.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDCLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27250..27495)
                     /locus_tag="U4A29_000265"
     CDS             complement(27250..27495)
                     /locus_tag="U4A29_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO0679419.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27610..28188)
                     /locus_tag="U4A29_000266"
     CDS             complement(27610..28188)
                     /locus_tag="U4A29_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO0679420.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28265..28795)
                     /locus_tag="U4A29_000267"
     CDS             complement(28265..28795)
                     /locus_tag="U4A29_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO0679421.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="U4A29_000268"
     CDS             complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="U4A29_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO0679422.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29652..30047)
                     /locus_tag="U4A29_000269"
     CDS             complement(29652..30047)
                     /locus_tag="U4A29_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO0679423.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30044..31456)
                     /locus_tag="U4A29_000270"
     CDS             complement(30044..31456)
                     /locus_tag="U4A29_000270"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO0679424.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="U4A29_000271"
     CDS             complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="U4A29_000271"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO0679425.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(32075..32314)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(32311..33771)
                     /locus_tag="U4A29_000272"
     CDS             complement(32311..33771)
                     /locus_tag="U4A29_000272"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO0679426.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33941..35098)
                     /locus_tag="U4A29_000273"
     CDS             complement(33941..35098)
                     /locus_tag="U4A29_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEO0679427.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35132..36502)
                     /gene="radA"
                     /locus_tag="U4A29_000274"
     CDS             complement(35132..36502)
                     /gene="radA"
                     /locus_tag="U4A29_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO0679428.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36565..37092)
                     /locus_tag="U4A29_000275"
     CDS             complement(36565..37092)
                     /locus_tag="U4A29_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO0679429.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37322..38128
                     /gene="proB"
                     /locus_tag="U4A29_000276"
     CDS             37322..38128
                     /gene="proB"
                     /locus_tag="U4A29_000276"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO0679430.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38125..39372
                     /locus_tag="U4A29_000277"
     CDS             38125..39372
                     /locus_tag="U4A29_000277"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO0679431.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39448..39792
                     /locus_tag="U4A29_000278"
     CDS             39448..39792
                     /locus_tag="U4A29_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679432.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39909..40235)
                     /locus_tag="U4A29_000279"
     CDS             complement(39909..40235)
                     /locus_tag="U4A29_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679433.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40608..41809)
                     /locus_tag="U4A29_000280"
                     /pseudo
     CDS             complement(40608..41809)
                     /locus_tag="U4A29_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41926..42441
                     /locus_tag="U4A29_000281"
     CDS             41926..42441
                     /locus_tag="U4A29_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO0679434.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42640..43086)
                     /locus_tag="U4A29_000282"
     CDS             complement(42640..43086)
                     /locus_tag="U4A29_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEO0679435.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43133..44611)
                     /gene="msr(C)"
                     /locus_tag="U4A29_000283"
     CDS             complement(43133..44611)
                     /gene="msr(C)"
                     /locus_tag="U4A29_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEO0679436.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(44965..45594)
                     /locus_tag="U4A29_000284"
     CDS             complement(44965..45594)
                     /locus_tag="U4A29_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO0679437.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45578..46042)
                     /locus_tag="U4A29_000285"
     CDS             complement(45578..46042)
                     /locus_tag="U4A29_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO0679438.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46529..47797)
                     /locus_tag="U4A29_000286"
     CDS             complement(46529..47797)
                     /locus_tag="U4A29_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO0679439.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48028..48765)
                     /locus_tag="U4A29_000287"
     CDS             complement(48028..48765)
                     /locus_tag="U4A29_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO0679440.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48832..49401)
                     /locus_tag="U4A29_000288"
     CDS             complement(48832..49401)
                     /locus_tag="U4A29_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO0679441.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49529..50401
                     /locus_tag="U4A29_000289"
     CDS             49529..50401
                     /locus_tag="U4A29_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO0679442.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50743..51615
                     /locus_tag="U4A29_000290"
     CDS             50743..51615
                     /locus_tag="U4A29_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO0679443.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51612..53570
                     /locus_tag="U4A29_000291"
     CDS             51612..53570
                     /locus_tag="U4A29_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO0679444.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53821..54672)
                     /locus_tag="U4A29_000292"
     CDS             complement(53821..54672)
                     /locus_tag="U4A29_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO0679445.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54795..55262
                     /locus_tag="U4A29_000293"
     CDS             54795..55262
                     /locus_tag="U4A29_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEO0679446.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55525..56007)
                     /locus_tag="U4A29_000294"
     CDS             complement(55525..56007)
                     /locus_tag="U4A29_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEO0679447.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56107..56970)
                     /locus_tag="U4A29_000295"
     CDS             complement(56107..56970)
                     /locus_tag="U4A29_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO0679448.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITKTQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57568..58416
                     /locus_tag="U4A29_000296"
     CDS             57568..58416
                     /locus_tag="U4A29_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679449.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58587..58928)
                     /locus_tag="U4A29_000297"
     CDS             complement(58587..58928)
                     /locus_tag="U4A29_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HEO0679450.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59014..59721
                     /locus_tag="U4A29_000298"
     CDS             59014..59721
                     /locus_tag="U4A29_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO0679451.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59708..60031
                     /locus_tag="U4A29_000299"
     CDS             59708..60031
                     /locus_tag="U4A29_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO0679452.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60120..61157)
                     /locus_tag="U4A29_000300"
     CDS             complement(60120..61157)
                     /locus_tag="U4A29_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO0679453.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61604..62587)
                     /locus_tag="U4A29_000301"
     CDS             complement(61604..62587)
                     /locus_tag="U4A29_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEO0679454.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT        17940 a        13388 c        11154 g        20330 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatttat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    16261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    16321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    16381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    16441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    16501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    16561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    16621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    16681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    16741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    16801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    16861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    16921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    16981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    17041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    17101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    17161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    17221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    17281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    17341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    17401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    17461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    17521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    17581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    17641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    17701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    17761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    17821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    17881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    17941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    18001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    18061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    18121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    18181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    18241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    18301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    18361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    18421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    18481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    18541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    18601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    18661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    18721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    18781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    18841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    18901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    18961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    19021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    19081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    19141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    19201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    19261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    19321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    19381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    19441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    19501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    19561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    19621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    19681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    19741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    19801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    19861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    19921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    19981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    20041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    20101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    20161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    20221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    20281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    20341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    20401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    20461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    20521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    20581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    20641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    20701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    20761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    20821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    20881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    20941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    21001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    21061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    21121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    21181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    21241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    21301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    21361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    21421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    21481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    21541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    21601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    21661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    21721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    21781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    21841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    21901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    21961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    22021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    22081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    22141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    22201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    22261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    22321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    22381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    22441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    22501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    22561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    22621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    22681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    22741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    22801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    22861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    22921 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    22981 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
    23041 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
    23101 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
    23161 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
    23221 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
    23281 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
    23341 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaacaatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    27241 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    27301 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    27361 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    27421 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    27481 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    27541 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    27601 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    27661 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    27721 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    27781 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    27841 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    27901 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    27961 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    28021 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    28081 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    28141 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    28201 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    28261 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    28321 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    28381 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    28441 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    28501 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    28561 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    28621 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    28681 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    28741 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    28801 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    28861 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    28921 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    28981 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    29041 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    29101 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    29161 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    29221 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    29281 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    29341 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    29401 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    29461 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    29521 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    29581 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    29641 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    29701 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    29761 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    29821 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    29881 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    29941 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    30001 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    30061 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    30121 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    30181 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    30241 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    30301 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    30361 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    30421 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    30481 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    30541 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    30601 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    30661 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    30721 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    30781 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    30841 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    30901 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    30961 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    31021 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    31081 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    31141 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    31201 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    31261 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    31321 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    31381 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    31441 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    31501 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    31561 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    31621 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    31681 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    31741 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    31801 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    31861 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    31921 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    31981 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    32041 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    32101 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    32161 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    32221 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    32281 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    32341 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    32401 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    32461 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    32521 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    32581 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    32641 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    32701 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    32761 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    32821 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    32881 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    32941 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    33001 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    33061 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    33121 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    33181 attataagtt ggcataccat cgcgtttttg tatgacaaag tcgccaccga tattatcgga
    33241 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    33301 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    33361 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    33421 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    33481 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    33541 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    33601 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    33661 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    33721 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    33781 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    33841 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    33901 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    33961 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    34021 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    34081 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    34141 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    34201 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    34261 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    34321 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    34381 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    34441 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    34501 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    34561 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    34621 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    34681 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    34741 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    34801 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    34861 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    34921 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    34981 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    35041 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    35101 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    35161 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    35221 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    35281 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    35341 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    35401 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    35461 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    35521 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    35581 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    35641 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    35701 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    35761 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    35821 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    35881 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    35941 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    36001 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    36061 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    36121 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    36181 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    36241 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    36301 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    36361 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    36421 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    36481 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    36541 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    36601 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    36661 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    36721 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    36781 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    36841 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    36901 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    36961 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    37021 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    37081 tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc
    37141 tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta
    37201 attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt
    37261 actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca
    37321 catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt
    37381 gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc
    37441 tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg
    37501 tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc
    37561 ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca
    37621 acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa
    37681 tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa
    37741 cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct
    37801 gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga
    37861 cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga
    37921 taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg
    37981 cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat
    38041 tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg
    38101 tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa
    38161 gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa
    38221 atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta
    38281 gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct
    38341 gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt
    38401 ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg
    38461 ccactaggcg tgaccggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct
    38521 agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc
    38581 tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct
    38641 tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg
    38701 acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa
    38761 gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat
    38821 aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca
    38881 tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg
    38941 ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg
    39001 ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta
    39061 gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat
    39121 cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt
    39181 ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac
    39241 ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt
    39301 ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa
    39361 atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag
    39421 gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac
    39481 cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa
    39541 ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc
    39601 gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta
    39661 gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg
    39721 attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg
    39781 actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact
    39841 gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg
    39901 gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg
    39961 tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt
    40021 tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca
    40081 gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct
    40141 ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt
    40201 gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt
    40261 aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg
    40321 gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa
    40381 ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc
    40441 cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaaaagct
    40501 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct
    40561 gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta ttcttcttca
    40621 tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag aacaatggca
    40681 cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc tgcaataaat
    40741 ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc aacgtttcgt
    40801 tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat tccttggaaa
    40861 aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc agctcctaat
    40921 aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata agtcgtaaac
    40981 ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac accccagcca
    41041 aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg cgcatacgca
    41101 agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga acgaagttta
    41161 tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg tttctcagtt
    41221 tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa
    41281 aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc aacagccatt
    41341 ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc aactaaaata
    41401 ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc ccagccggca
    41461 cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc gaatacaacg
    41521 atgataaaca atcctgtcat caatgttttt tcaaaccgat ccgactagat aagaaacctg
    41581 taaatagcat caccacacca gtgactaaca tgtaactagt aaataacagg gtcgtttcag
    41641 ctggcgtagc gtttaactgt gcagcaatcg tttttaagat tgggtctact agacccactc
    41701 ccataaaagc gatcatacag gtagctgcta caactagcgc tgaacttttc ccctttgttt
    41761 cttcatttgt ttgttcttgt atatctattt gattctccaa tatttccatc cctttctctc
    41821 tattagcatc atacatgatt tattttacat gtaaatatgc acatgtaaaa cgtggaaact
    41881 ttactgtgat ataataaaga aaataagacg agaggtagga tttttatgaa tactttagcc
    41941 tatatattat tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt gaagcagctg
    42001 atcacaattt tttgggaagc ccatcacagt cagatctata caacgcttgc caagttagac
    42061 cagcagggtt atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa gatctaccac
    42121 ttgactgatg aaggacgtgc tcttgtagag gaatggatca aagaggaaac tcccgctcct
    42181 acgcaaaaag atgaatttct agcaaaaatc tatgcttttt cgacgctcga taaaacaaca
    42241 gctactggac tgctattcac tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa
    42301 aaaaaattga atgacctctc gctagacaag aaacaagaat ttggacgtta tgttgtgatc
    42361 cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa
    42421 atgcagcagt tttttgagta agggataaac agaacctttc tagaaatatc tattgaagag
    42481 caaattatct tcaataaaag agcaatacag ttggaaaaga aaaagcgatc cgaacgacag
    42541 gccaaaacct atcgttcgga tcactatttt tctagaactg cattcaagag actatgacaa
    42601 aaagcatgcc aaggcctttt ttcaaataac agtgttccat cattcattac tttgaattgc
    42661 ttttagcaca cttcttgaaa gcgggccaac gatgatcaac tgcaacggta aagcggcaat
    42721 cacattcatt ttccaagcat tcatataagc agagaataat tgatcaggta ttccttgttc
    42781 catgatcaca ccgaataaag acatacatgt caccattcct aaaaccatca agcatgaaat
    42841 agttaagatc atttggacct tgctttctgg atttactggt agtttaaagg caattttttt
    42901 cgccagcaca ccgatgatga agacatccaa aatgaaagcc accacgaatc caggcactag
    42961 accaatcaac aaacttgata aatgaagatc ttgatgcagc cataaattat acgcgctcat
    43021 ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt attccttctt ttttatttgt
    43081 aggcaatctg taccactcct ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat
    43141 ttctcgtaag tacttttttt attttctgcc cttttatttc ataaacttca tcagccgtac
    43201 gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt tcccggatat atttgcatta
    43261 gcttctctaa agcttcgatc gttgccatat caataaaatt agtcggttca tctaacagca
    43321 acacattact tggcttagta aaaagcaaag caatcgtcag acgggtcgct tctcccccac
    43381 ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac
    43441 tacggacaag cgattctgaa aactctgttt gtctcatgac aaaacgaatg atggattctt
    43501 cagatgtcat ttgataaccc ttttgctgat atacttgaaa gcttactttc ggagagagaa
    43561 ggattccttc tccttgtttg cggatatgtt ctaataaggt tgtcttacct gaaccatttt
    43621 caccgacaat cgcgattttt ttacctaaag aaaactgaaa atcaccattt accagtaatg
    43681 ttctcccact tctttccaat tggacatttt gtcccatgat gggatagcgg ctgtggagtt
    43741 caagagattt tggtactgga aaacggattt gtttcatctg ctgcggcttg gttgtttctc
    43801 ctattttttg gagtcttctc tctaatgttt tcgcattctt ttggatggct ttttgtacac
    43861 tgtctttttg tttagaggaa gacaaccgat ccggtctgat ttgttgcttt ttctttttac
    43921 tggacacttt ttccaacttt tccgcttgtt ttttcttttc ttggatcgat ttttccaatc
    43981 gttttttctc tttcatgatc gactctgctt ctcgtagctg agtctttttc tccaattcct
    44041 tttgagtgag ataggcacta taattccccg aaaactctcg gatttctccg tcttttactt
    44101 cccagatttt cgatgctagc tcatctagaa aatagcgatc atgactaaca aaaaccagcg
    44161 taccataata ataacgaagc tcttcaatca gtcgatcaat actttttcta tctagatggg
    44221 ttgttggctc gtctagaagc atcccttgtt cataagaaga aagaagacgt atcaactcga
    44281 tctttcgttc ttcccctccg ctccatttcc gctctgaagt ttcttccagt cttaactgga
    44341 aatgactggc taattccaaa tttaggtcct ttgcttcagc gagataggtt aattgaggca
    44401 ggtagttgaa ggtgatctct gtttggattt tcccatgatc gggaaaaagt tcaccggcta
    44461 ttagtttgag caaagtcgat ttaccgacac cattttctcc tatgatccct atacgatctt
    44521 gctgatagac acgaagagaa tcaatagata gttctagctg gttgccaaag ctgacttgta
    44581 gatttgttat atttactgct aaattttcca tatgttttac actcctattt catgtaaaac
    44641 aacaattatc gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat
    44701 cttttcctac tctgaacgaa ataactatga ctaaatcacc aaatctgtca tagctttcac
    44761 ctattctact gtcgttcgaa aaaatggaca tactccatcc atgaaattgt tgtttataca
    44821 tgtcttttga atttatttag ttgttattca aaagttcgaa acgtaatttc atcgatgcag
    44881 tcatttttcc gacctccttt tttattaggt taccacacac gattcaaaaa aagcaatagc
    44941 tactgaatag taactgaaaa ccttttattc gaacgtagtg atcttttctg gtgctaaacg
    45001 aacagactcg taaccttctt ctaccgcttt tcctattgat aaaataataa ctggaacata
    45061 acggtcttta tccaaaccaa atgcctctgc caactgatct gcttcgaatc caccaattgg
    45121 atttgtttca tagccatgat cacgcgcaac taacatgaat tgcatagccg ctaagctaga
    45181 atcgactttg actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat
    45241 cgcagctaat tgttgatctc tgacttcttt tggcatcttc cctgattcgt aagcttggtt
    45301 atagatttct tcgccatatt cgtaacattg catatcgcca aaaatcatga ccattgcaga
    45361 agaagtatca ttttgtctcg tattgaaacg gatcaaagga cgcaagattt cttttgcttc
    45421 ctcgctttca gcgaccacaa agcgccatgg ttgcatattg acagaagaag gtgcagtcgt
    45481 cgcttctttg atcatttcca gcatttcttc gtgtgggatt ttgtatgttt catcatatac
    45541 gcgaacggat cttctaccag tcataattgt tgaaaaatca tttttttgtt ccattattat
    45601 taacctcttt cgtttctgtt gttatattgc aaagttgggt attcaattta tctaaaagtg
    45661 ttagaaattg tttcatttct acatcgctga aagccgggga tagcacggaa cctgtatctt
    45721 tatggtccat ttcacaatgc tcgattttct tctgggcttt ttctgttagc gctacataca
    45781 cttcccggtt gttctgagga tttctctgac gcgaaacata gccatttgct tccaatacct
    45841 tcagatgacg agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta
    45901 gcaatggatt ttcttctttc agtctcatca tgatctcgta tctagtaaga cttataccga
    45961 tattttcttc aaagatctgg acgatttttt gatcggctaa tttgatttga tataaaaggt
    46021 gactaatctc ttctaaagac ataatttcct cctttctaat aattgactcg tcaacgattg
    46081 acttgtcaat aatagaataa aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa
    46141 aacgctctca atagaaaagt atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat
    46201 gataaagaaa gcacaacgct aaaaccaaga ttattgattc ttatcaaact atagaagata
    46261 gctcttctgc agtagaaaat tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga
    46321 atcccgtcga atattagaaa ttggatcagt atgcttaaac agtaataaaa aagttatgac
    46381 aagatgttgt cacaaccttt tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat
    46441 aagttcaacc aaacaaaaaa catgaaaatc tgtttttgtt tggctgttct tactacttga
    46501 tgcagggcag ctttttcact acctcttttt aataaccata aaatcctatt ccttcatatc
    46561 tccagcccat cggtgttagc caatctcttt ccttagcact caaggtatag tgatgggtac
    46621 cagttttttc atttggatta aatagtcgat aaattggctt ggaaccacca gaataaaaac
    46681 ttagaccttc ataacgccaa ccgatttttg ttaaagagtt gatctcagat tgtgccattg
    46741 tgtaatgatg gtctccactg taaggattgt ataaacggta gacaggttgt ccgctgtttg
    46801 gtgcttgcca agcaatccct tcatattccc agcctacagt tggcagataa tttttttctc
    46861 cggcattcaa tgtatataaa tgttctcctg tatgacggtt atacagacga tacatcggag
    46921 aagttgaagt tggtgctgta tcatactgtg tcagattata ggtttcgatt acattgttca
    46981 atgtagaacc ataattaggc gcagtggcat aacgacctgt tagccaagcc gtagcatctt
    47041 tgtaagagtt cgtattgctt ttccatgcac cggaatagta gtaaacgcct ggctgaaaag
    47101 aagtcgtctt caataccgtt acgttatctt gcaatgattc tttgtacgaa ggatattttc
    47161 taaaagcggc attcaccgta atccattgcc cattcacata ttccgaagta ggcatcgtaa
    47221 cagattggcc attatagctt cctttaatcc caaatagatt gtaattaggg gcagaagcca
    47281 aagtactgtt cccccagcca ctttcaacga tggcttgagc aatcatgact gatgcatata
    47341 agtcattggc acttgccaaa ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt
    47401 gagaattagc agatgagcga gcataaaaag catagccagt atagttttcg aattcagaca
    47461 aatctgcttg atgggtatgt gtcaatgttt gcccatctgt caattggttc gtgatttctt
    47521 ctgcattctc tggattgttc tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt
    47581 ctgttgtttc agttgttggt tcttcggttg aaattgtatt ttctgagaca gtagctgact
    47641 gcaaagtact ctctgtatcg ttttcaattc ctgtttctga agaactcgat gtctgagttg
    47701 cgacagtttc tgtcgattct tcagctagca ctgttgtgtt ggcaactgct agatttccta
    47761 aaagaataac gctaaataat aaattctttg ctttcatctt ttttctcctt tgaattatga
    47821 aacttttcct aaataacagt gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa
    47881 ataacatttg tttctttttg gtaaatttgc tttttctaca taaaaagacg taaatgaaga
    47941 atattcttct ccattcacgc cttaaattga tggtcgtttg aataactatt taacaagtaa
    48001 ttctctttgg atattatccg ttatggatta tacttcctgt gtcgtcggac ggataatcat
    48061 gttactgaca gacatccggt ctggcgtttc tatcgcaaat gctacagcag aagctatatc
    48121 ttcggaggtc aatccccatt ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc
    48181 gctgatggtt tgatataatt cagtttgaac agctcctgga gagatgatcg tagatttgat
    48241 gttattttgc cgttgctctt gacgtaatcc ttccatgatt gcacggacag caaattttgt
    48301 tccgcaataa acggcggaat caggataaac aacatgtccc gccacagaat cagttgaaat
    48361 gatatgccca gatttttgtt tttccatgat tggaagtacg gcggaaattc catttaacac
    48421 acccatgatg ttgatatcaa gcatattttg ccattcatca cggtgacctt caactaatgg
    48481 agcggttggc atgattcctg cattattgta caatacatcg attcgaccga acttttccat
    48541 cgctaaatcg ataactgctt ggacttgtgc aaaatctctt acatctgctg tttgaatata
    48601 tagctcagct tctggtaaag attcttttat tgcttttagg cgatcttctc ggcgtgcagc
    48661 tataactagt ttagcccctt tttctgctaa tagcttcgtt gttgcttcac cgattccgct
    48721 tgaagcgccc atgatgacaa tcactttttc tgataaatca ttcattctat tctcctcctg
    48781 ttttgaaaaa tctcttgctt atactcacta tctcttattt catcttactg ttcaaataat
    48841 ccgttcgaca ggtttgacga ttttggcagg gattcctgca acgatggtat tgtctgggac
    48901 atctttcgta acagttgcat tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg
    48961 cagaatagtt gctcctgctc cgatccaagc atttttcttg atgtggattg gctttactga
    49021 caagcctctt cgttcttttg gatcggttag atggttcacc gtgatgaggt tgacacgagg
    49081 accaatcaat actgaatctt ctaaacagat gccacctaag tcaacgaaca tcacttgacg
    49141 attgataaag acatttttcc ctatactaat atgtttgccg aaatctgtat aaaaaggcag
    49201 cagaacagtc accgtatcat ctactttgtc actaatgata ttttctagcc gtttgcggat
    49261 atctgctggt tcatggtacc cagtattcag cgctgctaaa ggctcttcgt tttcagcttg
    49321 gatctgatgg atcacttcaa agagcggatc cgttactcga atctctttat tgacgatttg
    49381 tttttcgatg aggcttgtca ttttgctccc tccttttatc cttactttat aacggacggc
    49441 ttatcatgtc taatacttat aaaacatact taactattcc ttttaactat acttagtggt
    49501 atgctttttt aaaaacagga gggaaaaaat ggatattcgt atactgacgt attttttagc
    49561 agtagctaga gaaaaaaata tcagccgagc agcaaaatct ctgcatatca cgcaacctac
    49621 tctctcaaaa caactgaaag atttagaaga agaacttggt gtgacacttt tcactcgggg
    49681 ctcccgagag atacagttga cggattctgg tcgatatctt tattcaaaag gaaaagaaat
    49741 cttgtcgttg gtagaaaaaa cggcgaacaa tttaacaaat gacgcaatca tctctggaga
    49801 aatctacatt ggcggaggcg aaactaaagc aatctctttc atttctgata gccttcattc
    49861 gttgatcaag gcgcatcctg atatccagtt tcatttatat agcgggaatg cagatgatat
    49921 catagacaaa ctggatagcg gattacttga ttttggactc gtaatcgaac cgacaaacaa
    49981 acaaaaatac cagtcatttc gactgccagc ccaagatcgt tggggacttt tgatacatcg
    50041 aaatcatcct ttagcaaagc aaaccgtcat ccatccgcag gaactgaaaa ctgttccttt
    50101 gatcatttcc cggcaaacgt cagtggacag tcaactagct gaatggctag gtttcagcat
    50161 agatcagtta tcgatcgtcg gaacatataa cttattgtac aatgcttctt tgatggtcga
    50221 taacgggact tttggtgcat tagctctaga tgggatcatc aatacttatg ggacggaaaa
    50281 aatattcatc ccactcgatc cgccattaca agcttcactg aatctcattt ggaaaaaaga
    50341 ccagcctctt tctcctgctg ctcgtaaatt tttagatgtc ctaacggaga aatacagtta
    50401 agtagagttt ttttccttcg ctttcagctt tttaacaacg aataagataa gcaaaagatc
    50461 caacgattaa ataacataag tttcttaatt tatttcttcc ttttatttct gataattcca
    50521 gtaaattact ataattataa aaaaagaatt gacaaaacat aactgtctat ataaaattga
    50581 ttacgttaac aataattcgt ttcatgtgac tagatcatac taggcatgaa aaatctctga
    50641 ggaacatggt ttccctagga ggtttttcgt gtctattttt gtttggccgc aatcaactgg
    50701 tctagcaaac gtgaaaacca aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc
    50761 tttaatcaaa atgcagtatt ggtagatgac ggtggacagg aaaaagtagc tattggaaaa
    50821 gggattggat ttgacagaaa aagaaatgat ttgatttttg atcgtgatgt cgaacggata
    50881 tttgtcatgg aaccagaagg tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa
    50941 cgctttttat ttgcagcaga aacgatcatt gatcatgcgg aaactgtttt gatggaaaag
    51001 ttgaatgaac atgtattgat cgctttgacc gaccacattg ctttttctgc ggagaacatt
    51061 aataacggta tcgtcatccg caacaaactt ttaagagaaa tcgaagtgct ttacagtgat
    51121 gaattcagta ttgctcaatg ggcagtagag tatctgacaa aagagcttgg gattccatat
    51181 acttatgacg aagcaggata tatcgcaatc catattcaca gcggacgaag cggtcggaac
    51241 aataaccatc gcagtatccg tgaagtgacg attgtatctg acatcatcca tttagtggaa
    51301 tcagaattgg aaacagatat ccattcagaa ccattttcac tgaattattc aagactggtg
    51361 aatcatttac gactgcttct acaacgaacc catgcgcaac aatatgctgt tttagacacg
    51421 gaaatcgtag aaatggtcaa acgaaaatac ccagaaagct ataagatttc taaaaaaatc
    51481 cgtgtgttgc tgaccaaaga ataccagctc gcgatcacga aagaagaact gggttacttg
    51541 gccatccata tcgagcgttt aagaagcgca atcgtacaaa caaatgttaa caatcatgag
    51601 gaggaaaaaa attgaaagca tatctacaac gaatgggacg ttcccttcaa cttccagttg
    51661 cggtcttacc tgctgctgct ttactagtag ggatcggaaa ctggtgggca tcttatagta
    51721 acgacattgt cgctcatttt ttacaagctg gcggtaacgc agttttaggt caattacctt
    51781 tgctgtttgc cgttgggtta gcactaggga tgtcagtgga caaatcaggt gctgctgcct
    51841 tggcaggttt ggtcgcattt gaagttccag taaatgtttt aaaaactgat tcagtagcta
    51901 ctttattgaa tatcaaacca gaatcagttg atccatcttt tgctcagatc agtaacgtat
    51961 ttattggtat tattgccgga ttgattgcgg cagctcttta taatcgtttc cacgaagcaa
    52021 aattaccgat ggctttatct ttctttagcg gcaagcgtct agtgccaatc ttggctgcct
    52081 ttgtcatgtt gctgatcagt gcagtgctac tgctcgtatg gcctccggtt tactctgctt
    52141 tagtttcttt tgctaaattc attttgagtt taggctgggg tggtgcaggt ctttatggct
    52201 tcttcaaccg tttactgatc cctacaggat tgcatcaagc aatgaactcc gtgttctggt
    52261 ttgacgtagc aggaatcaat gatattggta aatttttggc ttctgaagga acaaaaggcg
    52321 taacaggaat gtatcaagct ggatttttcc caatcatgat gttcgggctt ccagcaggag
    52381 catatgctat ttacagaaat gcacgtccag aaaacaaagc aaaaacagct tccttgatgc
    52441 tagcagcagc ttttgcttca ttctttactg gtgtgacaga accacttgaa ttttcattta
    52501 tgttcgttgc ttggccatta tatgtcttgc atgcaatttt cactgggatt tctttagcag
    52561 tgagtgcatt cttccattgg acagcaggat ttgcctttag tgccggattt gtcgattact
    52621 tcctatcttt gaaaaatccg attgcgaatc agccattgat gttgatcgtc caaggattag
    52681 taacagcagt gatttactac tttggcttta actttgctat caaaaaattc catctgatga
    52741 ctcctggccg cgaggaagca gatctttcag atgacgatac tgcaacgact aattcagaca
    52801 ataaatatgc ggcacaagca agcaaaatct acgctgcatt aggcggagcc gacaatgtaa
    52861 cttcgatcga taactgtact acacgccttc gtttgcaagt caaagataca ggcctgatcg
    52921 atcaaaataa aatcaaagcc actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg
    52981 cgcaagtcat cgtaggtaca gaagtacaat tcgttgcaga tgagatggca aaactacatg
    53041 gtggtgctgc agcacgacct gcacaaacaa acaccgtggt taaaacagaa acatttgccg
    53101 gtgaaacgat cgaacaagat atttacgcta tcgcaaatgg gaaactgatt ccaatcactg
    53161 aagtttcaga tgatgtattc gcggaaaaaa tgatgggtga cggctatgct gtattaccag
    53221 aaaatggaga aatcttctct ccaatcgctg gtacgatcac aaacattttc ccaacgaaac
    53281 atgctgtcgg aatccaaaca gatgctggta tcgaagtctt gcttcatatg ggcatcaaca
    53341 ctgttgactt aaaaggcgaa ccattcactt tatatgtaga agaaggtcaa aaagtcgcaa
    53401 gaggtcaatt gattgcttta gttgatcttg ccgctattca atctgcaggc aaaaacacgg
    53461 atatggtcgt tgtattcaca aatggcgaca aagtccaaag cttagaaatc gaacccgcac
    53521 gtgatgtaaa agcaaatgat aaaatcggct ctgtttccaa caaagcctga gccatcgtct
    53581 tccccagact tttttatcga taaacaaaca ggagctgaga caaaaatctt gtctcagctc
    53641 ctttttccga ataaaaacgg cgttctcctg ccaactccgc ggtaataagt ccagctaaac
    53701 gaaacatatg aagagctatt ttttatttga ctcttcttat tacttgatgc agaacagctt
    53761 tttcacaaaa tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca
    53821 ttattcctga tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa
    53881 tactttttct aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat
    53941 cgctaaaaac atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa
    54001 cgctgcatca cttgcttcaa taatttctaa ctctttacct gtcgcacgct ctaatgcctt
    54061 ggctaattct tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact
    54121 ttctactagt acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt
    54181 tccatttcct gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa
    54241 ttcattctct acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat
    54301 cattttttca gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc
    54361 aaaacttgta taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt
    54421 atgttcggct tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt
    54481 caacgcttct ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc
    54541 ttctttcagt gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg
    54601 aaccatcttt tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac
    54661 taaataatgc atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca
    54721 tttataaatt acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga
    54781 aaggatgaca actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct
    54841 atattgaaat gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg
    54901 aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct
    54961 ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac
    55021 tagatgtcta tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa
    55081 atccaaattg cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc
    55141 tgcagaaaaa ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa
    55201 tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat
    55261 aattctctct actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt
    55321 ctccggggga caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg
    55381 ctgaagtcat gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg
    55441 aaaaatgcaa aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc
    55501 gctactttca cgacctctat attatcatgg tttattgact accggtatcc agatttctcc
    55561 catatatttc ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc
    55621 gtaatcttct acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa
    55681 ttcttcaggc gattctgcta aagcgcttac aacaagatac gttccttttg ggaatgggat
    55741 gattcgcgta gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt
    55801 cccgttatag ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc
    55861 atctattgtt ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc
    55921 gacaaaatcc gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg
    55981 tttttcttcg atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc
    56041 ttacataaaa ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt
    56101 ttttaactat ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc
    56161 ctctccccat tttgtaaaag agatctcggg agcttcgact aacccataat ctgaagaagg
    56221 aagccattct gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt
    56281 attaggaaaa atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg
    56341 attttctttt gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg
    56401 gtctccatca aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt
    56461 atgcatctct tccctttgag tcttcgtgat cgtctgcgct aacttttgta tttcttgatt
    56521 gactccttca aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt
    56581 cttttcgata cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg
    56641 tggaaaagat ttttgtatgc cgtatctact gatttctgaa ggcagatatc ctgaccaatc
    56701 acgaaaagct cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt
    56761 cactcttgat ccatctagaa gatcgcgatt agccaaagcc aatctacggt ttttgatata
    56821 gtccattagc gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat
    56881 tcctgtattt tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta
    56941 atcgatcaat tggttcagat ctttaagcat atttatcttc ctccttgatt gcctttttat
    57001 catattatca aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga
    57061 aaatgttgta tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat
    57121 tatctgccta tctatgtttt acctttagaa aaataaattt tggggaagac agattctaag
    57181 taaacaaagc ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta
    57241 caaagcaaat tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata
    57301 catcatacct cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt
    57361 tgtcagataa ataaaaaaca aaaaaagttc catctttaaa acacgctatt cttttacttt
    57421 ttctcacatt ctctaagaaa agaaaaggag aatgaatttt tgacaattcg agcaaaagtg
    57481 attatcaaat atgtaaaatc agctactttt ttcttaaacg tttctttata ctaaatataa
    57541 ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt tcctgaaaac
    57601 gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt gaactataca
    57661 agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt gatcacttgg
    57721 gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa
    57781 cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc caactactca
    57841 ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg ggagctttta
    57901 acagagattc ttatagaaca tccgcacttt atcgaacgct tcaaagttaa gatgccattt
    57961 catgcaataa attattcaga taaattaaca actatcatcc ataaatacga tcatattatc
    58021 aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa
    58081 gaagcagttg aatcgcttat tcagcaaaat gcctatcttc ccgcattcga attgatcaat
    58141 gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg aacccttctt
    58201 ctgatagagg actgttatta tttatggaaa gaattattag cagaagcacc tcatgcagaa
    58261 aaaaggcaaa tgttctcttg gttcgtctgc caagtcgatc atactgacgc ctcttattca
    58321 aaacgctact ctataaaaat cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa
    58381 aagaaaaaaa tagataaaaa actaaagaaa cggtaaagaa agacgatttc aatgaaaaat
    58441 agcaagtaaa cgataacaac catcctaaat ccacaagaaa tacagcagac cttgctttaa
    58501 aaacacgaat catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca atattagtca
    58561 tttgactatt aggtacgcgt tttgttttat aagtcatgac aaatttcctt cgttgctgga
    58621 tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt
    58681 tccgctgcat ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac
    58741 cctaacccta gtgcagcaat cattgttgcg cctaatgcag gtccttcctc cacacttaaa
    58801 caatagaccg ttgcatcgaa aatatcagct tggatttgta gccatgcaag atttttcgcg
    58861 cctcctccca ccgaaacaat acgtgtaaat ctgcgacccg ctagttcttt acgtttcaat
    58921 cccaccattg acaaatgctc attaaaaatt tagaatattc acaagtttaa cgacaattat
    58981 gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact
    59041 gcgatcaaag acacgttacc taccgttttc ggttatatcg gtattggact tgcatttggt
    59101 atcgttggga aagctgccgg atttcatcca ctagtcgtca cgttgatgtc cctgctggtc
    59161 tatgctggtt ctgcccaatt tatcacagtc agcatgcttg ctagtcacag cccattgctt
    59221 tccatcgttt tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt
    59281 gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca
    59341 gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt
    59401 cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt
    59461 ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc
    59521 gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa
    59581 cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt
    59641 atcccaagca acttagtgat tgtagttgta acactgattg ggtgcggatt gggggtgtgg
    59701 atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac
    59761 ttggctttct agggtcctac catttgtctt actgaaaaaa ttcgatttac cgcaaccgtt
    59821 gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct
    59881 atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt
    59941 tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggcaggaat
    60001 tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta tggcaaatta
    60061 ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg tattcttgct
    60121 tatccgttga acggaccact aacaacgact tttcctaaca tatgtcctgt ttctacgtct
    60181 ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt gctcaatgta
    60241 ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt
    60301 tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa gctgatagat
    60361 tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt tcccacatgt
    60421 ccaaatggtc ggacaaggtg cttgatttga tcaaagtagc gggtaatatc gaataataca
    60481 gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa gtcgtgatga
    60541 taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt cggacttgca
    60601 gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga accgacgccc
    60661 ccggatccgt tgatcactaa gatttttttc ccgcgattgg cattttcttc tggagttaga
    60721 ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa ggctgcagct
    60781 tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac tagttggtat
    60841 tcttgattgc ttcctgctct tgttgttgta cctgcgtaaa aaacgcgatc gccgacagaa
    60901 aatttttgca cttcgtcacc tacagcaaca actttcccga ctccatcaaa acctaagata
    60961 cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg attaacagat
    61021 actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg ttctttttca
    61081 tcaataaagc tttttggatc atcgatcggc aacccttcat aaaaaccgac taccttcatt
    61141 aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc atccatacag
    61201 tgttcatttc aatatatata gtatgctatt tgttccacct tttttattca tcataattat
    61261 tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac
    61321 ttttgtcaca acctcttata aacggtgttc tcctaccaac tcttcggtca caagtcaaaa
    61381 tattcatcaa acatgagaag ctgttttttg aaattgctcc ttgtgactca gagttaaacg
    61441 gcaggctcac aacctctgat ataaacggtg ttcaatcaac tgaccctcgg tattagctca
    61501 aaaaacgaca aaatatgaag agctattttt gtttggctgt tcttattact tgatacagga
    61561 cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt tcactgaaaa
    61621 gcgttggatg ttctttttca aaggtctcta agatctctcg tacttcatca gtagatttcg
    61681 tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata tagattttaa
    61741 agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga tcttgcagat
    61801 ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc gggtcttttt
    61861 caaatgcata aggattcttg aaaatccctc ggccaatcat gatgccatca acgccatatt
    61921 tctcggccaa ttcttctcct gtctggcgat cggggatatc tccattgatc gtgattagtg
    61981 tttgtggagc gatttggtca cgtaaagcaa tgattttagg aatgacttcc caatgtgcat
    62041 ctactttact catctcttta cgcgtacgca aatggacaga aagattggct atatcttgat
    62101 gaagtaggtg actgatccat tcttccatct cagacatttc cgtatagccg atacgcgtct
    62161 tgacgctgac aggcaaaccg ccagctttag ctgcttcaat caattgtgcc gctacttctg
    62221 gacgaaggat caagccactg ccttttccac gttctgctac attagggact gggcagccca
    62281 tgttgatatc gattccttgg aagcccattt ctgccatagc aatgctcatt tctctaaaaa
    62341 attctggctt gtctccccaa atatgggcaa ccatcggctg ttcatcttct gtaaatgtta
    62401 atcttcctcg tacactgtct tttccatctg gatgacagaa actgtctgag tttgtaaact
    62461 cagtaaagaa gacatccggt gctgctgcat gctttacgac atggcgaaat acaacatctg
    62521 ttacatcttc cattggtgct aaaacaaaaa aaggctttgg cagctctttc caaaaattag
    62581 tagtcattta tttgttactc tccattctat caagcagtga agtcgcaatc tttctcaaaa
    62641 acattgtcat tatactagga aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt
    62701 ctgattgagc aaaaaaaaga ctgtgacaaa aatcttatca cagtccattt tccggaataa
    62761 atggtgttca aaagcttgca tttgcggtaa taagtccaac caaacgaaaa at
//
LOCUS       DASOUD010000005        57844 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.005, whole genome shotgun sequence.
ACCESSION   DASOUD010000005 DASOUD010000000
VERSION     DASOUD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.005"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="U4A29_000302"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="U4A29_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO0679455.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="U4A29_000303"
     CDS             853..1041
                     /locus_tag="U4A29_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO0679456.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="U4A29_000304"
     CDS             1054..1611
                     /locus_tag="U4A29_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO0679457.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="U4A29_000305"
     CDS             1643..1885
                     /locus_tag="U4A29_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO0679458.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2226..3440
                     /locus_tag="U4A29_000306"
     CDS             2226..3440
                     /locus_tag="U4A29_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO0679459.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3471..4898
                     /locus_tag="U4A29_000307"
     CDS             3471..4898
                     /locus_tag="U4A29_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0679460.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4921..5847
                     /gene="glsA"
                     /locus_tag="U4A29_000308"
     CDS             4921..5847
                     /gene="glsA"
                     /locus_tag="U4A29_000308"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEO0679461.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6055..7515
                     /locus_tag="U4A29_000309"
     CDS             6055..7515
                     /locus_tag="U4A29_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679462.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7736..9499
                     /locus_tag="U4A29_000310"
     CDS             7736..9499
                     /locus_tag="U4A29_000310"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEO0679463.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9583..10041
                     /locus_tag="U4A29_000311"
     CDS             9583..10041
                     /locus_tag="U4A29_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEO0679464.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10150..10833
                     /locus_tag="U4A29_000312"
     CDS             10150..10833
                     /locus_tag="U4A29_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO0679465.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10980..11594
                     /locus_tag="U4A29_000313"
     CDS             10980..11594
                     /locus_tag="U4A29_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEO0679466.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11610..11816
                     /locus_tag="U4A29_000314"
     CDS             11610..11816
                     /locus_tag="U4A29_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679467.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12355..12912
                     /locus_tag="U4A29_000315"
                     /pseudo
     CDS             12355..12912
                     /locus_tag="U4A29_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            12970..15009
                     /locus_tag="U4A29_000316"
     CDS             12970..15009
                     /locus_tag="U4A29_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEO0679468.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(15118..16269)
                     /locus_tag="U4A29_000317"
     CDS             complement(15118..16269)
                     /locus_tag="U4A29_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO0679469.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(16423..16953)
                     /locus_tag="U4A29_000318"
     CDS             complement(16423..16953)
                     /locus_tag="U4A29_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO0679470.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(17052..17402)
                     /locus_tag="U4A29_000319"
     CDS             complement(17052..17402)
                     /locus_tag="U4A29_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEO0679471.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(17559..19088)
                     /gene="ahpF"
                     /locus_tag="U4A29_000320"
     CDS             complement(17559..19088)
                     /gene="ahpF"
                     /locus_tag="U4A29_000320"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO0679472.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(19098..19661)
                     /gene="ahpC"
                     /locus_tag="U4A29_000321"
     CDS             complement(19098..19661)
                     /gene="ahpC"
                     /locus_tag="U4A29_000321"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO0679473.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(19805..20467)
                     /locus_tag="U4A29_000322"
     CDS             complement(19805..20467)
                     /locus_tag="U4A29_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO0679474.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            20651..22297
                     /locus_tag="U4A29_000323"
     CDS             20651..22297
                     /locus_tag="U4A29_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEO0679475.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            22288..23370
                     /gene="uxuA"
                     /locus_tag="U4A29_000324"
     CDS             22288..23370
                     /gene="uxuA"
                     /locus_tag="U4A29_000324"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO0679476.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDIGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(23478..25031)
                     /locus_tag="U4A29_000325"
                     /pseudo
     CDS             complement(23478..25031)
                     /locus_tag="U4A29_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25179..26320
                     /locus_tag="U4A29_000326"
                     /pseudo
     CDS             25179..26320
                     /locus_tag="U4A29_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(26446..27525)
                     /locus_tag="U4A29_000327"
     CDS             complement(26446..27525)
                     /locus_tag="U4A29_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO0679477.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(27628..28068)
                     /locus_tag="U4A29_000328"
     CDS             complement(27628..28068)
                     /locus_tag="U4A29_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO0679478.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(28125..28535)
                     /locus_tag="U4A29_000329"
     CDS             complement(28125..28535)
                     /locus_tag="U4A29_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEO0679479.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(28715..29149)
                     /locus_tag="U4A29_000330"
     CDS             complement(28715..29149)
                     /locus_tag="U4A29_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO0679480.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(29249..30097)
                     /locus_tag="U4A29_000331"
     CDS             complement(29249..30097)
                     /locus_tag="U4A29_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO0679481.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            30486..32252
                     /locus_tag="U4A29_000332"
     CDS             30486..32252
                     /locus_tag="U4A29_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEO0679482.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            32354..33613
                     /locus_tag="U4A29_000333"
     CDS             32354..33613
                     /locus_tag="U4A29_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO0679483.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            33760..35061
                     /locus_tag="U4A29_000334"
     CDS             33760..35061
                     /locus_tag="U4A29_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0679484.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            35064..35915
                     /locus_tag="U4A29_000335"
     CDS             35064..35915
                     /locus_tag="U4A29_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0679485.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            35928..36743
                     /locus_tag="U4A29_000336"
     CDS             35928..36743
                     /locus_tag="U4A29_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEO0679486.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            36894..38060
                     /locus_tag="U4A29_000337"
     CDS             36894..38060
                     /locus_tag="U4A29_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO0679487.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            38290..38937
                     /locus_tag="U4A29_000338"
     CDS             38290..38937
                     /locus_tag="U4A29_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO0679488.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            39021..39965
                     /locus_tag="U4A29_000339"
     CDS             39021..39965
                     /locus_tag="U4A29_000339"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO0679489.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            40100..41551
                     /locus_tag="U4A29_000340"
     CDS             40100..41551
                     /locus_tag="U4A29_000340"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO0679490.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            41572..42408
                     /locus_tag="U4A29_000341"
     CDS             41572..42408
                     /locus_tag="U4A29_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO0679491.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            42408..43067
                     /locus_tag="U4A29_000342"
     CDS             42408..43067
                     /locus_tag="U4A29_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO0679492.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            43193..43882
                     /locus_tag="U4A29_000343"
     CDS             43193..43882
                     /locus_tag="U4A29_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679493.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            43888..44844
                     /locus_tag="U4A29_000344"
     CDS             43888..44844
                     /locus_tag="U4A29_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEO0679494.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            44888..45400
                     /locus_tag="U4A29_000345"
     CDS             44888..45400
                     /locus_tag="U4A29_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0679495.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(45656..46204)
                     /locus_tag="U4A29_000346"
     CDS             complement(45656..46204)
                     /locus_tag="U4A29_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO0679496.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(46293..47474)
                     /locus_tag="U4A29_000347"
     CDS             complement(46293..47474)
                     /locus_tag="U4A29_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO0679497.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(47710..49218)
                     /locus_tag="U4A29_000348"
     CDS             complement(47710..49218)
                     /locus_tag="U4A29_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEO0679498.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            49377..49568
                     /locus_tag="U4A29_000349"
     CDS             49377..49568
                     /locus_tag="U4A29_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679499.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            49771..50688
                     /locus_tag="U4A29_000350"
     CDS             49771..50688
                     /locus_tag="U4A29_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEO0679500.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            50681..51754
                     /locus_tag="U4A29_000351"
     CDS             50681..51754
                     /locus_tag="U4A29_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679501.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            51902..52420
                     /locus_tag="U4A29_000352"
     CDS             51902..52420
                     /locus_tag="U4A29_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679502.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            52401..52793
                     /locus_tag="U4A29_000353"
     CDS             52401..52793
                     /locus_tag="U4A29_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO0679503.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            52869..53804
                     /locus_tag="U4A29_000354"
     CDS             52869..53804
                     /locus_tag="U4A29_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEO0679504.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            53937..54716
                     /locus_tag="U4A29_000355"
     CDS             53937..54716
                     /locus_tag="U4A29_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO0679505.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            54709..55026
                     /locus_tag="U4A29_000356"
     CDS             54709..55026
                     /locus_tag="U4A29_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO0679506.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            55270..56880
                     /locus_tag="U4A29_000357"
     CDS             55270..56880
                     /locus_tag="U4A29_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO0679507.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            57037..>57844
                     /locus_tag="U4A29_000358"
     CDS             57037..>57844
                     /locus_tag="U4A29_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO0679508.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTH"
BASE COUNT        19240 a        10214 c        11296 g        17094 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctttatt ccttatgaaa aaatatcttt aaaaataaaa aaagacgaaa ataaacggtt
     2041 ttctataaaa agaagaccgt ttattttttg tctttatttg tgtgaaaaac gaaaaagagc
     2101 acaacacatc aagcttttat taattgtctg tcttttataa atgcaagtat taattcaaaa
     2161 aaacagaaga aaaaaacaac tacaccttct tttatactcg taatcgtaga aaggaggcga
     2221 aaaaaatgaa tacattacct atcaatacag gagatacagc attcatgatt ttatgtacag
     2281 caatggtttg cctgatgact cctggtctag ctttttttta cggcggatta gcacggaaga
     2341 ataatatact aacgatcatg tctcaatctt ttctctccgt tgggataaca acgattatgt
     2401 gggtatttgg tggattcgga ttggcctttg gacgggatat cggcggtgtt attggaaacc
     2461 cggcagattt ctttttgatg agacatgtta caagcttccc caacatgttt catggtgcaa
     2521 cgattccatt tttaatgttc tttctttacc aactgatgtt tatcgtgatt accgttcctt
     2581 tgatgacagg agcttttgca gggagattga atttaaaagg ctatattata cttgttattt
     2641 tttggaatct gctgatttat ttccctgttt gccactggat atggggtggc ggatttttgg
     2701 atcagatggg atttagagat ttcgctggtg gagcagtcat ccacacgaca gctggattcg
     2761 gctctttagc atgtatcctc acacttggtc aaagaaagat tcctttagga gaaagaagtc
     2821 agcatagcag tcttatggca gctgctatcg gtacaggact gctttggttt ggctggttcg
     2881 gttttaattc cggtggtgca cttcgagcag atgaacaagc agtcaatgca tttggttcaa
     2941 cattcatcgc tcttgcattt gcgatgatta cttggctgat cattgcaaaa gtaaaaggaa
     3001 atggctttga ttttgtcgac gtactaacag gatccgttgc tggattggca gccattacgc
     3061 cttgtgccgg ttatgtagaa gcaaaaagtg ccatgctgat tgggatcatt gcgggtatcg
     3121 tctgtcatgc tgctgttgat tttcgtaaaa agaaacaatg ggatgatgca cttgatgtgt
     3181 ggggtgttca tggcatgggc ggattcacag gtacgattct gatagggatc tttgcatcta
     3241 acagccattt actaacgaat ccaagcagct ggtacttctt agggattcaa gtgattggtg
     3301 tcatcattac tgccgtgtat gcctatgtgc tgacaacagt gattttaaaa tcggcgatgc
     3361 actttacaac gatcactact acaaaagaag aacaagaaga aggattggac tttttcttct
     3421 ttggcaacaa actcaattga aacaaattca aatagaaaag aggaagattc atgcaacaaa
     3481 gcaaaacgaa aattcctgca aaaaaattaa cgttctttgg ctttttggct atgaccatct
     3541 caatggtcgt ttcattatat gaatatccca cattcgcgac ctcaggcttc agcttagtat
     3601 ttttcttgtt attaggcggt ttgctatggt ttatccctgt ggcattgtgc gcagcagaga
     3661 tggcaacggt gaaaggatgg gaaaaaggcg gtgtctatac ttgggtcagt cgcacattgg
     3721 gaaaacgatt cggttttgcc gcaatctttt tccaatggtt tgagataaca gttggctact
     3781 tgaccatgct gtatttctta actggtgcat tatcctatgc aacgggtatt ggtgctatac
     3841 aaaacaataa atatctgaaa ttggcaatcc tattgattat tttctgggct attttactct
     3901 cacagttaaa aggaaccaaa tacacttcat tgattgctcg gatcggtttt gtagctggta
     3961 tcttgttgcc agcattagtc ttgtttggct tagggatcca ttatgtcgct tcaggcgctc
     4021 cactacaaac gatactgtct atgaaaacat tgattcctga tttttcgaaa ttgcctacgc
     4081 tggttgtttt tgtttccttt attttagcat atatgggcgt agaaacatct gccagtcatg
     4141 caaatgaaat ggaaaaccct aaaaaggatt atccattagc aatgtttgta ttggttattc
     4201 tggcaatcat tttagatact tttggcggat tgaccgtcgc tactacagta cctcaacatg
     4261 gtttgtctct taatacaggg gtgattcaat cgttagaatt cctattaaga cacattgatc
     4321 caaatcttgc atggattgcg aaaattcttg cgattcttgt ttgtttaggt gtcatcggag
     4381 aaattgcttc ttgggtaacc tctccttcaa aagcacttca cgtagcagca gaagaaggat
     4441 tacttcctga atattttgca aaagaaaaca cacacggtgt tcctgctcat ttgatgatcg
     4501 caaatgggat cgttgcaaca atctgggcag ccgtctttac attaagcggc ggggggaata
     4561 atctttcatt tcttgcagcg atgtcattga ctgttgtaat ctacctgatg ttgtacttta
     4621 tgtttttcgc gggctatttg aagctggttt ttaaagatga cagtcttgaa agagcctatc
     4681 aggttccagg cggaaaagtt gtaaaagtaa tcgttgcttg tttaggtctt ttgacttcac
     4741 tttttgcttt ttgtatctca tttgttccac ctgctagtat cgcagctgga cagcatggga
     4801 tttatgaaac gattctgatt gtttcttatc ttatcgtctt agttattcca tttacgatct
     4861 acgcatgccg tcataaattc cagcatgcag aaaaataaaa aatgaaagct ggttattcaa
     4921 atggaagtaa aagaattaga acaattgatt ggaaaaaatt tccactatta tcaaacagga
     4981 caagtggcgg attacattcc agcattagct gaagtggatc ccaaacaatt aggtatggcg
     5041 atttatgatt tggatacaaa acaactgatt gagggaggag acgccgatgt gcgctttgcc
     5101 atcgaaagca tgtcaaaagt tcccgtattg ttattagcaa tcaaagacaa cggaatagaa
     5161 aaagtctttc aaacaatcaa cacggaacca accggttttg ccttcaattc tatcatgaat
     5221 atggaaatca atcatcgcaa acatccaatg aatccttttg ttaatgcagg agcgattgca
     5281 acaacctcgt tgattgccgg taaagatgca gatgaaaaat ttgagcgaat cttgtcattt
     5341 atgaaagaga tctgtgatga tcctaatatc acattgaatg aagaaatcta tcattctgaa
     5401 tcaagaacag gtgatatcaa tcgctctctt gcttactata tgaaaggtaa tcaaatgatc
     5461 gaaggagacg taccggagat actcgatgct tatttcagac aatgctccct caatgtcacc
     5521 gctacaggca tagcaaaatt agctgctgtg ctggcaaata aaggaattgc tccttggaat
     5581 ggcaaacaaa tcattactga agaaagtgct acaatcgtca aatcaattat gacaactgct
     5641 ggtttgtatg atgaatcagg agagttttcc gtccatgttg gtgtacctgc aaaaagcggt
     5701 gtaggcggcg ggttgatggc agctgtgcca aatcgttacg gcataggtgt cttcagcccg
     5761 gcactagatc catttggtaa cagcgcagct ggtatccaat tgttgaaaga tgtagtcaaa
     5821 gaactagacg ctgacatctt tgaataacca ttcttgaaag aggctggggt aaaagtccaa
     5881 acggctcaaa gctacctaca atgaggctga aattgtgagc ttgtttgact tttgtcccag
     5941 tttctttttt tattggtttt tgaaagggct tttctaaata aaaaggaaac aaaaatacga
     6001 tttacagata tgattaaata aaatcataag aaaaactgga ggcgtgtatc aggaatgctg
     6061 gaaaattaca tcgagcgaaa tatatttaga aaagtgtatc tgtgtgaaca attatttgaa
     6121 ttccaagaaa tagacatcga acaaacagct atttctctaa gggtgactac accaacgatc
     6181 cttcacgatc tagaaagtct agccgagtgt ctagaatatt gtataaaaga acaagtaaga
     6241 gagaagcata agtataaact cgtattcaaa catggtattg ctctttctga gctcacacag
     6301 tttctgtacg gtcaatctta ttttttaaag tttttaagtt attattttaa tggtatattc
     6361 acgagtacag aactggctga tcttgaattt atttctttga gcaaagtata tacaatcaaa
     6421 aaaatcgtat tggatttttt taaagaaaat gattatttga aaaataaaga aatcattatc
     6481 ccagaatttg actcgcggaa catcttgcta gctttagtac gttatgtcaa ttgggaaggc
     6541 tatgaaaata aaaatccgta tgtagaagaa gcttgtaacg agttgattga ttatgtagag
     6601 actcattttt ttaaaagaag atattcggaa gatgaaaagt cctttatcat acgaggaata
     6661 gagatcgcaa tcggaagaaa ggcatttccc cttggactga aagaagaaga taagaaaagc
     6721 gcagaaacga agccgttatt tcaattagtc tgtcaaggat tggaagagca aaaaggaatt
     6781 ctagacttac aagaagaaga tgtctattat attttttctt tgttcaacag tagaaattat
     6841 accaatgaga acatggagct gttaagaaaa gatttcgaag ttgtttataa aaattttgtt
     6901 ctcaatgacc tatcactgaa tgaattgatt ttacagatta ttagtatcgc tgaaatcgac
     6961 gatgcagaaa attttatttt caagcaatcc tttttacaat ttgtccgtac gttgtgggca
     7021 gatggacaag tatttttacc ggaaaggatc tatcttctat cagaaaaaga acaacttctg
     7081 tacggaaaaa ttgtagaaat attggaagag tggaaaaaga aaaatcaaat ccaactaaga
     7141 tggaatgaaa atttcattcg aaaattcgtc aaaagtcttt ctttgatcaa cagtgaacaa
     7201 gcggggagac aggaaataga aatttttgtt gtttcagaaa gttcagtcaa acaatttttt
     7261 tatcgaaccc aatttcattt gtatgcggaa gaaggcatcg ctgaaataaa tcctatttta
     7321 tttcgttcat tagaagaatt gcctgatgaa tgtctatgtg cgaaaaaaag agtggttttg
     7381 tgcgatgctg ctagctatca aaatggatta gaaactgaaa agacaaagat ctatccaatc
     7441 tcattaaaag gtgcaaacat ccatttgtat catattgctc aggaacttca cctattaaaa
     7501 aaacatattt cttgatgaga ccttaattag gagaccttct acaaagcttt ttaattgtct
     7561 gtagaaggtt tttatttgag tcctgattcg tgcgaagatc tacttatata acaagctttc
     7621 tctgaaattt ttttaaaatg ctattgactt agagtcaact ctaagtagta gaatagataa
     7681 agtgtaacaa atctattttc attaaaagaa gatagtacgg aggagagaat gtattatgag
     7741 tacagtatta catgtattgg atgcaatcaa tagccccgca gatgtcaaaa aattatctgt
     7801 tgaggaaatg aaacagttgg cagaagagat gcgccaaaaa atcttgcaaa aggacagcga
     7861 tgtaggtggg cacgttggtc cgaatctagg tgtagtagaa ttaacgattg cgtttcatta
     7921 tgtatttgat tcaccaaagg ataaaatcgt ttgggatgtt tcccatcagt cttatggtca
     7981 taagctttta actggtcgaa aaaatggttt tgaaaaagga cattaccgtg aagtgagcgg
     8041 atattccaac caacatgaaa gtaaacatga ctattttaca gtggggcaca catctacatc
     8101 tatcagctta gcaacaggtt tagctaaagc gagagatctg aagaaagaat caggaaatgt
     8161 ggtagcattt atcggtgacg gctccctaag tggtggctta gcttttgaag gattgaacaa
     8221 tggtggtgtt ttacaatcca atctaatcgt attagtaaac gataatgaga tgtctattga
     8281 tgagaatcat ggcagtttat ataaaaactt agctgagctt cgtcaaacca acggtaaaag
     8341 cagcaataac ctatttaaag caatgggatt tgattatcgt tacgtggata atggaaacga
     8401 tctggaaacg gtgatccgtt tatttgaaga agtaaaagat attgatcatc cgattgtact
     8461 tcacgtccat acggaaaaag gtcgcggtta tcagccggca atcgataata aaatgaaata
     8521 tcattggcat gtaccatttg atctgtccac cggggaggca aagcaagtaa cttctggaga
     8581 aacatttaat atgatcatca tggattattt ggataagaaa atgcaaacaa gtacagctcc
     8641 tattgccgca gtcaatgcag cgataccagg cgcctttgga ctgaaaacat tccaagaaaa
     8701 acatccagat cattattttg acgtggggat cgctgaacag cactctatca ctttcgcttc
     8761 tgctttggct tctcagggag tacgtccagt cgtcttccaa tcaagtactt tcttgcaacg
     8821 ggcatatgat caattatcac atgatttagc gatcaacgag aatccagtga ttattttagt
     8881 cggtggcgga acgatctcag ctagtgaccg aacacaccaa ggaacatttg acattcctta
     8941 cttatcaagt atcccgaaca tcaaatatct tgctccagca acaaaagagg atatgatctc
     9001 tatgctagat tgggcgttcg aacaaacagc gcagcctgta gcaatccgaa ttcctacaca
     9061 tggtgtagaa catggtacag ctatcgcaaa tgattatagt attccagcct atcaaacagt
     9121 aaataatgga ggaaaagtag caattttagc tttaggcggt tttcttgaat taggaaaaga
     9181 aacagtaaaa gcttttagaa aaaaaggaat cgaagcaacg ttgatcaatc caggaacgat
     9241 cacagaaaat gacgtagaaa cattagataa attaaaagaa acacacgaat tagtcatcac
     9301 attagaagat ggtagtttgt caggaggatt tggcgaaaat atcagtcgtt attatggtga
     9361 ttcttcaatg aacgtattaa attttggtgc agagaaagaa ttcacggata gcgttcctgt
     9421 agggcagctt tatgaacgtt atcatttaac accaaaacaa atcgttgaag atagtctgcg
     9481 tgtcttgaat attcaataat tcattatgct caccatccag ataattgttt atactagtat
     9541 ctggatggtt ttttcattat aaaaagcaaa ggggtggatg ttatgtatca gcaagtagaa
     9601 gaatatgtca aaaagttcca agaaaaatat cctagttatc aatcagaaaa attaggtttc
     9661 ttttcatttg gggatacaga aaaaatggca gacgagttga gcgaactagt tgtaaaaaag
     9721 ataaagactg gtacatcaag tggttacgat ctgtataaac aagagaatga gcctttacca
     9781 aaagttggtc aattagacgt ggtcctgaat ggaaaaaatg aaccagtttg tatcatcaaa
     9841 aatataaatg tgaccatctt gccttttagt gaagtcacga aagagcatgc ttggaaagaa
     9901 ggagaagggg atcggacatt agcttattgg cggaaagtcc atgctgagtt ttttaaacat
     9961 gcttataaag aagcgttgaa catagaattt acagaagaga agctggtcgt atacgaagaa
    10021 tttgaagtaa ttttctttta gtaagaaagg tctgctgaaa gaaatagctc attattgtct
    10081 cctgaataga aaagggttac atttttatat gaagctgtat ttttatataa cattactgga
    10141 ggagatattg tggatacact ggaacaaatc aaaggcggag tcgtcgtttc ttgtcaagct
    10201 ttggaaaacg agccgctaca cagttctttt attatgggga gaatggcttt ggctgcaaaa
    10261 gaaggcggag cagttggtat tcgagcaaat acgacagaag atatcaatga aatcaagcga
    10321 atcgttgatt taccggtaat agggattatc aaaagagatt acgatgggtc agaagtgttt
    10381 attactgcga cagaaaaaga agtggaagaa cttttggaga cgcaagcaga aatcattgca
    10441 ctggatgcaa cttctcgtaa acgtccgaca gaaaaaacaa ctgctgaact tgtccaaatg
    10501 attcacgaac acggtcggtt agctatggca gatatttcga cttttgagga agcgcttcaa
    10561 gcgcaagaag atggttttga tcttttgtct acgactttag caggctatac agaatattca
    10621 cgaaagacgc agacacctga ttttgattta ttgaatcaaa tcatagaaca agtcgatatc
    10681 ccagtaatta tggaaggaca tactgactct cctgaacaag tagagaaagc atatgaatta
    10741 ggtgcttttt cagtcgtagt tggatctatt atcacccgtc cgcaattgat cactaaaaga
    10801 tatgtcgaag ccgcaagtaa agcaaataac taaaaaacac gcacggcaca gattcttgac
    10861 aaaaaagaaa aattgataga tagtttaaag cagattctaa gaagatgaaa aaagcattac
    10921 ttttcatttt gtcttcaaga tctgcttttt aacgaatttt atagaaagaa ggcgaaacca
    10981 tgtttgaacg gagtccttca gaattgacag aacgtgaaaa ttacaagttg ctgattggtt
    11041 cagtcatccc tagaccagta gcagtagtaa cgactaaatc agcaaaagat atagtgaata
    11101 tcgcaccctt cagttacttc aatatcgtca gttcgaatcg acccatctta tccttggcga
    11161 tccagagaaa acaagatgca gtaaaagaca cagcaaagaa tattcttgag acaagagaag
    11221 ctgttatcca tatccttgat gaggacaacg tagaagaagt aaataaaaca gcagccaatc
    11281 tgcctaatga ggaaagcgaa ctgtacctga ctgacttgac tttaactgat tcagtaatca
    11341 tttcagtacc agcattgaca gaagcaaaag ttcgttttga aacatcctta tatgaacatg
    11401 tcgagatacg gaataaaaat aaagtaacag ctgatttatt tttgctagaa gtacagaaat
    11461 atcatttaaa tgaaacagtc tatgatgata agacaggaag gatcgatcca agaaaattga
    11521 atgctgtaag ccgtttggca ggaaatgatt atgcagcaat aggagaaatt ttcacgattg
    11581 cacgtcccga ataaaaagga gcactagtta tgttaaaaat aagctataaa ccatcgacag
    11641 attcaaaaga aatgaaaaaa gaatatgaaa cagtgaatga ttttctgcaa gggcagtact
    11701 tagaagttcc gccattgcaa gaccattttg tcgttactac tgttacttta gatggtaagg
    11761 aaatagaaat gccagatcaa acgattagcg gattgtttaa ttactttaac aaataaatat
    11821 ttattccgaa aagtgaaaaa acatataaaa aaacacataa aactaaaaaa ttatcaaaga
    11881 atcctggtgc aactgaaaga atttgcaacg ggattctttc aacttatttg ttattttttt
    11941 ctaaaaaata ttaaagattg ttaaatttca ctatttcatt tcaaattcca ttgcctaatc
    12001 attaggaaaa gtctatcatg aactcgtaaa tagaatatag taataacctt gttaggtgag
    12061 gctcctatac aaacataggc tgttgcccag aaacgtcgaa agtcgccaat gggtaaaaca
    12121 gaaactgttg gattaaggct ttttccaata gctaagaatg ttttctttac gttgtatagt
    12181 gctaaaactc ggacgatgag atgatgctag gtcagaattc ttgttcgaaa aggtttctga
    12241 ccttttcttt atatttttgt gactgactta tttgatgaga caaactcgct cctcacctga
    12301 caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa gatcgattgg tggaggtcgc
    12361 tcggattcac ttctggttgc acaatacaac cagaaaacac aacaagcaaa actgatctct
    12421 atcatgagag attcatatgt cgatattcca ggttacggaa tgaataagat aaatgcagct
    12481 tactcttatg ggggagttga tttattaaat caaacattaa aggaaaattt caaatttgaa
    12541 gctccctatt atgcaagtat cacatttcaa gattttatcg attgcgttaa tgaactgttt
    12601 cctggtggag tcaagatcga tgcagaaaaa tctctagatt tagatggcgt atatataaac
    12661 aaaggagagc aagtgatgga tggtaatact ttactccagt atgctcgatt tcgggaagac
    12721 aaagaaggag acttcgggag gatcagaagg cagcagcaag tgatcaaagc tatctctcag
    12781 cagctgaaag acgtaacttc aatattcaag ctacccaaag cggttgggaa attacttggt
    12841 agtatccaaa cgaaccttcc agaaagtgtg cttctggatt gtggtatgga tttcctcaaa
    12901 gacatatgtt agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag
    12961 gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc
    13021 ggcgcagtga tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa
    13081 gaaacccaag cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac
    13141 aagggagatt ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca
    13201 ttatctgaaa aagagatctt agataaatac cagaatatat acggtgctgc tgatgtcaaa
    13261 ggacttcaga tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca
    13321 tataaagcaa agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca
    13381 ttagacagaa atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa
    13441 atggaaggaa atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata
    13501 gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca
    13561 agtaaacttg gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc
    13621 gacttaacag aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac
    13681 tttgtcccat tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt
    13741 caagaagtag acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac
    13801 gtgggagata tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa
    13861 atcggtcgct caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtggg
    13921 aaattaagca ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt
    13981 caaaatggca aagatatcaa attgacaatc gatgcgaagg cacaaaaaat agcttttgac
    14041 agtctaggag gaaaagctgc atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt
    14101 gcgcttgcta gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa
    14161 gattacaaag cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt
    14221 tatgctcctg gatctacgtt taaaatgatt acagcagcaa tcggtctcga caacggcact
    14281 atcgatccaa atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg
    14341 ggatctttta aaacaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct
    14401 ttgatctatt ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa
    14461 tttcgtacag gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg
    14521 aatccagcac aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact
    14581 ggatatggac agggcgaact tctaattact cctatccagc aagcagcaat gtattctgtt
    14641 tttaccaaca atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat
    14701 aagaagaatg tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt
    14761 gtgcaagatg taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg
    14821 aaaactggta cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc
    14881 ttgtttgctt tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa
    14941 gaagatgatg attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat
    15001 tatcaataaa aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc
    15061 cttttttcaa ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca
    15121 cacttcataa actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa
    15181 taaaatcacg ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc
    15241 aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa
    15301 tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt
    15361 caaaatcgct acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa
    15421 atcaacttct aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc
    15481 ctctacattg tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa
    15541 aaaagcacca atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca
    15601 aataactagc gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag
    15661 taggaaaggc gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc
    15721 aatcaaaaca agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct
    15781 gaaacttaga actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga
    15841 tccttctttt gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga
    15901 agttgccgca agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt
    15961 gcctgtcaac cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc
    16021 aggtcggaaa tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa
    16081 taagatcaca ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa
    16141 tccagcttgg cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa
    16201 tctccgcgag atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat
    16261 aaattccatt ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat
    16321 tattgaaaaa ataggaaaat atactcacaa aaaaaccaac aatccaaaag atttttttgg
    16381 attgttggtt tttatacggt ctattttaat aactaaaaat gattaacgta tagtcatttt
    16441 tcttattctg atgaaattga taatctcctg gatgcccatg aaaatcaaaa taaagccgaa
    16501 tatttggaat agaagcatca aacttttgaa tggattgaac aataagacga atccgccaat
    16561 caacaacaga atactactat aaacactcca acttttagct tttgtattca tgctgaagaa
    16621 caactggaac aagctgttga tgataacagc taatccaaga agaatcggca ggatagaaac
    16681 gattgcaggt gcaaaagcat agacgatcag agctaagatc aggaaaaaaa gaccactgaa
    16741 cagaccgata ctccaagaac ctgttttcct tttaaaacgg taagcctcaa aaagattcaa
    16801 caacccaagc aaaacaagat aggcggtgat cagataaccg ataaaatgaa aaacagcaga
    16861 agggtttagc acaatcgcaa tcccagaaat aatataaaca gcagctctta ataaggcgta
    16921 tttttgaata ttttgtttaa tcgtctcaaa catataagaa aacctccttt gacttatttt
    16981 aacgtgaact agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat
    17041 ctctggaaaa atcaacttct gaggatcttt tccatttttt tgccttttgc tagctcatcg
    17101 atcattttat ctaaataacg aatttctttc atcaagttat cctcgatgtc ttctactcgt
    17161 acaccacaaa caacacctgt aatcaaggaa cgtgaaggat tggggacagg ggcttccgca
    17221 aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt ctagttctgt ttgggaataa
    17281 cccgtcagcc aacaaatgat ttcatctact tcctctttta tacgatcttt tctctccgct
    17341 tttgctacat acataggata aacgcttgca aaacttgtcg taaaaatacg attttgcgcc
    17401 ataatcagtc acctttcttt ttttcaaatt atttactact tatagtatat aagaaaaaga
    17461 gaagaaaaag cgattattca aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc
    17521 cttttttccg ctctctgact ttttacaatc ttcatagctc aagtgcgcat cagataatcg
    17581 aatgctccta aagctgcagt agctcctgaa cccattgaaa caatgatttg tttgtatgga
    17641 ctatctgtac aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg
    17701 gtcactattt catttcttgg attcatttcg atcgttctat ctagccaatc cgtattaggg
    17761 agtaagccga ctagaatgaa tactcctgaa acagctagtg tatgcatctc catcgtacgg
    17821 cgatcgatat aggagagaga ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca
    17881 tgtttgataa cagtgacatt cgcaagagag actagtttat cctgtaagat ttgatctgct
    17941 tttaattctg gtaagaattc tagaacatat acatgttttg ctaacccagc caaatcgatt
    18001 gctgcttcaa caccagaatt accaccaccg ataacagcaa tctcttttcc tttaaataaa
    18061 gggccgtcac agtgtgggca ataagcaatt cctttatttt taaactcttt ttcgcctggt
    18121 acattgatag aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat
    18181 ataccgttag ctaagccaat attaataaaa tgttgctctt tatttaagga aacggcctgt
    18241 tgattcatca taatgtcgat agggtaacta cgcacatgac tttctacttg ctgcatcaat
    18301 tttggacctt ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt ttcaacgact
    18361 tgtccgccga aagaatcagc gacaagtcct gtacggattc ctttacgtgc tgcatagata
    18421 gctgcactac tagctgctgg tccaccgcca atcactaata catcataagg ttctttttca
    18481 aacaactctt cttcaatcaa agggccagtg attttttcaa caatttgttc gatcgtcatg
    18541 cgtccgctac taaattcttc tccatttaaa aacacagttg gcactgccat gattttttta
    18601 tcatcgattt ctttttggaa catcccacct tcaatcatcg tatgagtaat atttggattt
    18661 aatacagcta atatattcaa tgcctgtaca acgtcggggc aattatgaca agtcaagcta
    18721 acataactgt caaaatgcaa tgtttgtgtt atttttttga ttttaagact caaagattca
    18781 ctgattttcg gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga ggaaaattca
    18841 tgaccgaggg ggataccagc aaaaacgatt ccactttctt tgccaactgg attgatagaa
    18901 aaactaggtg tgcgcatcga atctcttttt tcccaagaga ttttaggaga catttctgct
    18961 atttccttcg taaattcagc tagtttttga ctattttcac tattatccaa actaacagaa
    19021 aaaacaatag gagattcaat cagtgctaaa tattgttcga gattttgttt agtttctttg
    19081 tctaacatat ccaccaccta aatctttcca acaagatcaa aacttggttt caatgtttcg
    19141 ccactttctt tccattttgc cggacaaact tcacctgggt gggtacgtac atattgagca
    19201 gcgcgaatct tatcaatcag tgcactagcg tcacgaccga ttccatctgc attgatttcc
    19261 atagcttgaa cgattccgtc tggatcaata ataaatgttc ctcgctgagc taatccttgc
    19321 tcttcatcca aaacatcaaa aatacgagaa atctgatgag acggatcacc gatcatcacg
    19381 tattcgattt taccgatagc atctgatgta tcatgccatg ctttgtgagt aaagtgggta
    19441 tcggtagagc aagaaaagac ttcaacacct aaagatttca atgtactgta ttgttcttgt
    19501 aagtcttcta attcagtagg acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata
    19561 ctccagttcc ccataagatc tttttcactg acttctaaaa attctccttg atgataagca
    19621 tgtgcattaa attcttcgat ttttttccca atcaatgaca tagtaaaacc tccaataata
    19681 attatttatt tataattatt gtaatttagt tggagttgca aagtcaatgg atattttgtt
    19741 atttaataaa gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa
    19801 aatgctactg ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca
    19861 tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag
    19921 ccttgagtaa atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca
    19981 ctcgtaacca gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt
    20041 cttttcttcc tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag
    20101 cgcgttcgtc atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta
    20161 ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt
    20221 ctcggacaaa gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac
    20281 tttgaggtat cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg
    20341 gagtccggcc gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt
    20401 ctaagtcagc atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc
    20461 ttttcatctt tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga
    20521 aaaacaatct ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg
    20581 acatagcgat ttttattgca taaaattcac ttgaatacaa aaactaatat gttagtttag
    20641 gaggggaaaa atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa
    20701 agaagtagaa atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc
    20761 tgtctggatc catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca
    20821 acgtttagcg gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga
    20881 agaaataatt gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc
    20941 aagcagtgga gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca
    21001 tccagattgt tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca
    21061 attagttact tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata
    21121 tataggaccg attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag
    21181 tattcttgct gtgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt
    21241 ctctacagat aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc
    21301 aaaagaatgg caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga
    21361 agaaattgtt gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc
    21421 agttagcaaa atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa
    21481 aggaaataat attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga
    21541 tcattttccc aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc
    21601 aacggtagat aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc
    21661 tttagctatt atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc
    21721 agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc
    21781 tccaggtatc attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa
    21841 tccaaatatt cctaatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat
    21901 tcgttatggt gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact
    21961 tgtattcata ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca
    22021 aggaaaacca ttcatcccga gtccagaccc actattgaca gaccttcagc ataagctttc
    22081 cgatattcaa ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt
    22141 gtcaaataaa gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga
    22201 agaaatgttc atcaagatgg tagcaggacc aaatgccgta cgaaaaacat tacaggatta
    22261 cttacaagaa catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag
    22321 gaagaaaaga ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc
    22381 acattgatgg atataccagt aggagaggta tggcctaggg agaggatcaa agaactaaaa
    22441 gaagaaattg agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat
    22501 gccattaaaa ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc
    22561 cgaaacttgg cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac
    22621 tgggtgaaat ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata
    22681 tctgatgata ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat
    22741 gaatttggat tgccgggatg ggaacctgag cgcttagctc ctatcaaatc cttgcttgaa
    22801 gcctatgcat cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca
    22861 atcatcccaa catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca
    22921 tactcgattt ttggattagc tcgaatcgta aagaatcgag aagatttaga ttggttgtgt
    22981 cgcatagtag acagccctta taacggaatc actctttgta cagggagtat tgcagaagat
    23041 ccagaaaatg atgtatatgg gattttagca gaattttgcc aaagagatcg aatcccattt
    23101 gcacatgttc gaaatattaa atttttagct aatcgagatt tctatgaggc accacataaa
    23161 tccgaatacg gttcattaga catgtaccgc atactaaaaa caatgtatga catcggattt
    23221 aacggataca tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga
    23281 tacggcttat atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc
    23341 gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat
    23401 aagaaggatg ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt
    23461 tatgtatctt atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt
    23521 gcttcttctt ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct
    23581 tctggtgtga tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc
    23641 aaagggcagt cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc
    23701 gattcatttg aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca
    23761 cgcgtatagt tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt
    23821 actaggtctt caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt
    23881 tgattttgtt ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaat
    23941 acgctatgtt cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta
    24001 agaaatccat tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt
    24061 tcaggtataa acaaatcaaa tacagtacaa agggactcca ccatttctct tgaataaaga
    24121 acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat
    24181 tcgattttcg tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa
    24241 attggcagat taggtcgcaa aatatctaca gagagatcaa taaaaaagtc aatatattca
    24301 tactctgtga attctttttg tacgtctttc acagaagttg caggaaaatt ctctttacat
    24361 atttttaata gaaaatataa tttatctatc ataccgctat ttagtcggtc aagtacttct
    24421 tttttaaaaa aagtattcat cataaaagcc actacttgcg gatcttcatc ataaaattct
    24481 gtagcagtac ttgaaaagat gttccaataa atatctaatg ctgcatattg aatttgtacc
    24541 tcttttccag aaaaagactt tctgccatta ttagaaaagg agatagacaa accgtactat
    24601 gctaaaaatg cattaatctt tttcattttc gtatacaaat aagaggcact atagtttgtt
    24661 tgctgactga aactgataat cgaatttgtc tgacagctga acaaagaagt caagatctga
    24721 taactgactg attcgttgca gtatcttcca aatagacgat aatagcagta agccggatca
    24781 attccttcaa aattcaactt taaaaaaggt ttttgcttat caatatgcag ttctgttaga
    24841 ttgagacgct caaagtcttg ttctaattct ttgatgtata gtaaaaaatt tgattcagac
    24901 atatggaaat gatctgctaa atattgtgta gtaataaaga aacgatgttc agataggaaa
    24961 agaaaaatat ctaattttgt attgcttttt ttcttcaaga taaacgtttt aatcgcttct
    25021 tgagtttgca tgaaagaggc tcctttttac ttaaattgtt aaaacttgaa aatattactg
    25081 tttttttcat aaatttagac aaaaaaacag taaacatata ggtagaatct ctactatctt
    25141 atttcttgca tacaaaaaaa tcaaggagtg agagagttat gagatattca accatcttaa
    25201 tatcaacatt tgcagcctta agcttatcgc ttactataaa tcagacagtc cacacagagg
    25261 agacagaaac agccaaatgg acagaaacca agtgggccaa cgtcaatcga attacctttc
    25321 aggatgaatt ttcatccttc aattcaggag tcgcaaaatt aagaaaagga caaacagcga
    25381 ccatcaaagc aaaaattgac tattcgggag acttagcacc tatactacaa tcagctgtta
    25441 catttgagaa tgttgatccc actttgtcga ttggctatga cgagaacact ttgactatcc
    25501 aacacaagca agcgatattt accttcaccg ttacattaaa tcaggacttg ttaaaaccag
    25561 cgctttttac tgtcaaggta tcagatggac taccgaacga tcatcattta acgccgtata
    25621 gtcagcggca gacaattgag caagattcag ccatcgactc aggaggagat cttgttgaag
    25681 aaccaacaga taaaccagaa aatgaaaata agccagaagt accgcctaca gaaaatccag
    25741 acggagaaca gaaaccggaa atagaacctg gagaagaacc cgatacagaa actcaaccag
    25801 agccagacaa taaatcaaaa cctgaaataa cccctggaga aaaaccagat gtaactccag
    25861 aaccagatac ggattctgga aaccaaacag taccggaaac aaatccagac acagataatg
    25921 aaactgaaaa tccagaaaaa ccagaggtag atccagaaga aaaaccagat gtgactccag
    25981 aaccagatac ggacgcaaga gaccaaggaa taccagaaaa aataaacaaa aaaacaattc
    26041 aagaggatgg aaaaaaagag agtaaaaaat caaatctagc aattctaaaa ataaatgaag
    26101 agcagctgaa taaaaaatca cgaatatttg atagcgccca atcagctgaa accttaaaaa
    26161 gtagcaagga tacaaccttt gctgtcctga aacgaaaaat aaacagctac caaaatccgg
    26221 agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta tagcaacaat
    26281 gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata atgtctgtga
    26341 acgcattttt caaataaaaa gaaatatcca gaaaatgacc ccaaaatacc agattgaaat
    26401 ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag ggatatctgt
    26461 ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa agaaggtaaa
    26521 gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa caagtaagac
    26581 cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg gattcagcac
    26641 ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa catctcggat
    26701 gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa cacctgctac
    26761 tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat gaaagccaaa
    26821 tacagccaag gcgattgtag tgagcaacgt atttaaaaga gcgacaacaa attgagcttc
    26881 taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat cttgaaagaa
    26941 ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt tcttttcaat
    27001 cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt ttcctatatc
    27061 ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa aatcgtttcg
    27121 ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct gatgctcgta
    27181 aaaattaata acggactgga tcatgtcaaa tgtttgatga ataagcatcg ggatatactt
    27241 tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag ctaaaacacc
    27301 tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca aataagtaaa
    27361 gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa caaaaacgac
    27421 aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct gataaacggt
    27481 caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat gatggaatat
    27541 gaaaataaga aagagcaaaa agaaaaaccg ctaaatgacg ttatcttatg agttggtttt
    27601 cctcataaaa tcgttcatct agcagtttca gccctttttc tggtcttgat gaaaattatc
    27661 tagcattttt cgggtcagat gttgcagtgt atccaattct tcctgttcaa gtggaagata
    27721 agacttgatc ttttgtggaa taccttctgc ttgtttttgc aacgcagcac cttgagcagt
    27781 cagcgtaaca tttacgattc tttcatcacc cgtttcacgc tgacgattaa tataattttt
    27841 ttctgctaac ttttttagta agggagtcag cgtaccagaa tccagacaca aatcatttcc
    27901 tagctctttc actgaagtct ttttcttttc ccataaaact aacatcacaa gatactgggt
    27961 ataggtaaga ttcagcggct tgaggagggg agtgtagtgt ctaatgattt ctttagaggc
    28021 ggcatataaa ggaaaacata cttgattaac taaatataga tcttccattt ttccacctcc
    28081 gctgttttac tatagtgtat catagcggat ttacacaaaa aagtctattc actaactgct
    28141 ttcagagtaa cttctatatt tccagctgta gcttttgaat acggacacac tttatgcgct
    28201 gcctctaata attcttgcgt ttcttccaga gaaagacctt cgatagatcc ttcaatctct
    28261 acacctaaga caaagctcgt ttcactttct gaataaaggg aaacttgtgc actgacagta
    28321 gaagccccag agatattttt ttgctgtttg atcaattcta atgcactgtt aaaacatgca
    28381 ctgaaaccag ctgcaaacaa ttgttcaggg ttggttgcat tttcgacgcg tttaccagga
    28441 gctgcaatat tcaattcgaa tgtacgatcg ggattatgca cttctccaga gcgtccgcct
    28501 gtgttgatca ttttcgtaga atacattttt ttcataaagt taccaccttt aattatattt
    28561 tatacaattg aattttacac aatataattg tggaaagcaa agaaatggat tggcataaga
    28621 taaataagag aaagcacatg aaacagacgc gctttctcga atagcaaaca tatacaaaaa
    28681 atatcagaga caaaaaataa tctcatcata aacattacat atatttctca aaaagttgtt
    28741 ttctttcttc aaaaattact tcacgatgga cctgatgaag catccataaa taaccaaaag
    28801 gatctctgaa cattgagtta gtcacaccaa agtccatcat ttctgtcatt tcttgaatct
    28861 ctacacagcc atttcgtaat gctttattat gtgtgtcctc gatatcagag acgatgatat
    28921 tgaaccagat cgtatttgga tgttcagcag taggagcagt caaaccaaga gacgtgtttt
    28981 catccagcat atggaatctc gtaccataca aggtaaaaat gacttcatta ttgcctctag
    29041 ggtaatctgt tgcctcaatc agttgtattt catcaaagat tgtttgatac agttctagtg
    29101 ctttcaaact atcatttaat acaaaatcga tttccgttcc tttatgcata aagatctcct
    29161 ctccttttca gtcgaacagt tactagattc ttttattata ccagctaaag aaaacgcatt
    29221 caaaaaaata gcggatttac ttaccaactc attcgattgg atgataagta aatccgcgtc
    29281 ccattacttc tgctacgctg ctgacaatga caaaggcact tggatcgatt tcttctatca
    29341 atggtttcat tcgggaaaat tcatgatcag tcacaacgat caataaaacc tcttttgact
    29401 ttcgattata cccgcctttt gcctctagca gggtaaggcc acgtccaatc tcagcactta
    29461 agcgaaggcg gatgtcttcc aaataatgtt cactcatgac cataacagat ttctttcgat
    29521 tgatacctgt ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat
    29581 aaagaaactc ttctattcca aaaataaaca gattaagaga aacaacgaat gcgtctgtaa
    29641 aaaataagcc aatcgaagta ttcaagtgga aatatttttt aaaaatgagg ggagggatcg
    29701 tcgtaccgcc gctagaagag ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg
    29761 cacttccaaa aatgacagac aacaaccgat ctgaagtaat cattgtttct ggaactattg
    29821 caatgattaa aggaaaaacc aagcttccga aaagtacttt aaaaaagggt tttttaccta
    29881 aaaatagaag agctagtacc aacataacga tattcaacac taaaataact atagctcggt
    29941 caaagcctaa agcagcttcc agtaatattc ctagaccgct gacacctcca gcagcaatat
    30001 gatgaggccc taaaaacata ttgatagcga ctcctaatat gaagagggca aaaataatct
    30061 taatgtatgt ccatataaat tgtctcgttt ctttcattta ttcacttcct taattcatta
    30121 ataatactct tatcttatta taacttactt ttcaccttaa gttaacttta attaataaaa
    30181 aaattaggct agtctaaatt attatgtttc attcttctag aatatacttc tacgccaaaa
    30241 actgttaaaa aacttttact ttagaaaatt ggagaaattg atcgcttaag ataaattcag
    30301 gacactaaat agtacaggaa gtagactaaa acttttaaaa gttttaagtc tatctatttg
    30361 gcaagtttaa aaaataaaaa ttcggtatca aaaaactgtt accgataaca tttttgaaag
    30421 cgttgtcttt tgcttctcta tcacttatag tgatgacgtc atattaaaac acaaggagga
    30481 aatcaatgga tacagctgca atttatcatc gaccggaaag tgaatatgca tttctatata
    30541 cgaatacaag atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg
    30601 tgctatgtgg agatccatat accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa
    30661 tgaaaaaatg tctaagtacc aatgttcatg attattggga aattgaagtg acgagcgaaa
    30721 caagtcgctt acagtatgcg ttccatgttg ttggggaaga taacacggac tgtttttacg
    30781 gtgatcaagg aatttttcct tatcgagaaa atgtgattac tgaaccaaat ttttattttc
    30841 gaatacctta ttttcatcaa atcgatcgat tcactattcc agaatgggta aaagaaacag
    30901 tatggtatca aatttttccc gaaagatttg caaacgggga caaagcaaat gaccccgaac
    30961 acactttacc ttggggaagt aaagatccag accgagaaga ctttttcggg ggagatttgc
    31021 aaggtgtgat ggatcatttg gattacttga cggaccttgg catcaatggc atctattttt
    31081 gtcccatatt taaagccaca tccaatcata aatacgatac gattgattat tatgaggtgg
    31141 atcccgcttt tggcgataaa aaacttttaa aaaacttgat tgatgaagca cataaaagag
    31201 ggatacgtat catgttcgat gctgtattca atcatatggg tgtccattca ccacagtggc
    31261 aagacgttct caaaaacgga gaaaaatcga tctataaaga ctggttccac atccgttttt
    31321 tcccagtaga ttcttatcag atgactgact taccagaaac tgctgaaaat atcccatatg
    31381 atacttttgc ttttactcca ttcatgccga aactaaatac agcaaatcca gaagtgcaaa
    31441 aatatttgct ggatatcgca acttattgga taaaagaatt cgatattgat ggatggcgtc
    31501 ttgatgttgc taatgaagtc gatcatcatt tttggaaaaa attcagagaa gcagttacag
    31561 aaatcaaacc agacatctat atccttggag agatttggca ttcctctcaa gcttggctgc
    31621 aaggggatga attccatgca gtgatgaatt atgcatttac agattcgatt aaagattatt
    31681 ttgcaaaaaa gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc
    31741 tttacagaga tcaggtgaac gaaggaacat tcaacttatt agattctcat gatactgcca
    31801 ggatcttgac cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt
    31861 ttcttcaaaa agggtctcct tgtatctatt acgggacaga aatagggatg actggtgaag
    31921 atgacccgga ttgccgcaaa tgtatgatat gggagaagga ggaacaagat ctagagctat
    31981 tcggttttat aaaagaactt gtttctctaa ggaaacagtt gtctaagatt atttctgaag
    32041 gttctactca gtggctaata gtaaatgatc gtgaagataa gctctatttc acacggaaac
    32101 tagagggaca gatcatctat gtttacttta atcagtcaaa agaaccttgg gtagtagaac
    32161 aggaaaatga ggtgattctt agtcaaaatt gtcagttgtt agaagacggg aaagcagaga
    32221 tacgacaata cggatttttg atcatgtgtt gaaaactgtt accggtaaca acaagaaagc
    32281 gtttgcaaat gaagatttgt gcccattata ataagggtgt aacagcacgt tgaatacaaa
    32341 ggaggaaatg aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt taatctcttt
    32401 aggaatctta ggcggattag cggcttgtgg caatggtaac tcttcagcag aaggggaagc
    32461 cgatgaagga aaaacgttaa cagtttccgt tgattctgga tataaagatt atataaacga
    32521 aataaaaaac actttcgaaa aagaaaacga tgtgaaaatc aagctagtag aaaaggacat
    32581 gttcgatcag ttggaatcat tagcgttaga tgggcctgct ggaaagggac ctgacgttat
    32641 gatggcagca tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa
    32701 actgggaaat gaagcagctt acgatgaaac agacaaagcc caagtgactt atgatggaaa
    32761 aatctacgga gaacctgcag taatcgagac ccttgttttg tactataaca aagatttagt
    32821 cgatacagca ccatcaacgt ttaaagattt ggagaatcta tccaaagaca gtcgttttgc
    32881 ttttgagtca gaagcaggaa aaaatacagg atttttagct aaatggactg acttttatta
    32941 ttcctatggt ttaattgcag gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc
    33001 ttcagatatc ggtttgaata atgcaggtgc tgtagaagga atctcttatg caacagattg
    33061 gttcaaaaat gtttggccaa agggaatgca agacataaaa agtgcaggag acttcgccag
    33121 ccagcaattc atgtccaata aaactgcagc aatcattgac ggtccatggc aagcgcaaac
    33181 atacaaagaa aacaatatca attatggtgt agccaaaatt cctactctaa ataatggaca
    33241 gccttatcaa ccgtttggcg gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa
    33301 agacctctct caaaaatggc ttgattatgt aacgaatcag gaaaatcaag aaaaattctt
    33361 tgaaatggta aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa
    33421 cgatgaatta acaacagctg tgattgaaca atatgaaaca gctcaagcga tgccaaacat
    33481 cccagaaatg ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg cagcatcagg
    33541 ttcaaaaaca ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg aagccataga
    33601 acaaaaatac tgattaaaat gataaccgtc catttttata aaaaatggaa caggtgcttc
    33661 agataaaagt ggcaagagcg agatggaaaa ttatcttgct cttgttgcca ttatcttaaa
    33721 agcagtttgc ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga taaaacaagc
    33781 aatatcaacg aaaaaaaaat cacgctttgt tcacttattc cagggatggg gcagtttgta
    33841 aatggccaag cttttaaagg tatcgttttt ttcgccatat tcgtcctatt cattggtcag
    33901 atgcttctgg gcggatttca tgcattggta ggattggtta ctttgggaag tataccaatg
    33961 gaagatcatt cattatttat tttgattgaa ggcactttgc aattcatcat tacaatcatt
    34021 ttcttgattt tttacggttt gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa
    34081 gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac ataatatagg tgatgaaggt
    34141 tttccttatc tattgacact tcccgcttat cttctcatga tttttacgat cattttccca
    34201 gtcctagtta cattgttcac tgcatttact aattatgact tcaatcatat tccaccagcc
    34261 agtttaattg attgggtagg ggctaaaaac ttttttagta tcttcttttt aagctcatat
    34321 cgtaatactt tttttgcagt cttttcgtgg accgtgattt ggactttatg tgcaacaact
    34381 ttgcaaatca cccttggtat tctcacagcg gttgttgctc accagaaatt tattaaattc
    34441 aaacgtattt tcggtatcat tttcttatta ccatgggcgg tcccagcttt tattactatc
    34501 atgagttttt cgaatatatt caacgatagc gtaggtgcca tcaatactca agtgattcct
    34561 ttcataaacc agcttccttt tgtagatatc agtgcaatct cttggaaaac ggacccgaac
    34621 tggacgaaag tagcaattat cttagtgcag ggctggttag gatttcccta tatctatgtg
    34681 atggttacag gaatcttaca atcgatttca gaagacttat atgaagcagc aaaaatcgac
    34741 ggagcaaatg catggcagcg cttttcaaac atcacgcttc ctgctgtctt cctagtggca
    34801 gcgccaacat ttattacaca gtatacgggg aatttcaata atttctccat gatctatctg
    34861 tttaacaatg gcggtcccgg aagcgtaggc ggaggagctg gttctactga tattcttatt
    34921 tcctggatct ataaacttac gactggaact tctccgcagt attctatggc ttcagcaatc
    34981 acgttgatta tctccgtcat cgttatctgt gtgtcattgc tggtatttaa aaagacaaat
    35041 gcgttcaata tgggggatta acaatgaaaa aatttcattc gagtgctcga ttccaaaaac
    35101 gactttctca cttttttact tacttttttc ttttaatact gtcagtcatt atcatctatc
    35161 cgcttttgat cacagcaagt actgccttca agtcaggaaa tattgcagct tttagtttag
    35221 atctttcaag tgactggact ttaggaaact ttactcggct attcagtgaa acactttacg
    35281 ggacttggta taaaaataca ttgatcattt ctgtcctaac aatgatcgta caagtgactg
    35341 tagtaacttt ggcgggctat acttatagtc gctatcgttt tatcggccgc aaatcaagtt
    35401 tgatgttctt tttagtgatt cagatggtac cgactatggc agcattgact gccttttatg
    35461 tcatggcttt cttattagaa gcgctcgata agtactggtt cttgacaatg atctatatag
    35521 gcggtggaat tccaatgaat acttggctga tgaaaggata ctttgacact gtaccaatag
    35581 atttagatga atctgctaaa cttgatgggg cagggcattt caggattttt ttccagattg
    35641 tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt atgggacctt
    35701 ttggggattt catgttagct aagttcctgc ttcgaacacc tgaaaatcag actgttgctg
    35761 tcggcttaca aacatttatt tctgatgtga caaaccaaag agtctctctt tttgctgctg
    35821 gggcaatttt gattgcacta ccgatttgtc tattattctt ctttttacaa aagaactttg
    35881 ttaccggcct gacatctggc ggaacaaaag gataggagca gagtatcatg caaacatttc
    35941 cgttaaacta tttttccgca ttacgtacgc caactcaagt gtttgcggga cgcaaactat
    36001 tatcatggcc taaatttttc ttgatatttg tatttttagt ttccttgatg gtcatgcctg
    36061 tgactctttt ttatgccaat cagatccaag cgattccatt agaacaattt ctatccgttc
    36121 attcgctgat cgatgaacaa ggaacccaaa aattttctga acttgaatta tctgaaactg
    36181 ggttacaagc atcgcaacaa acgatagctg tgacccctga aattttggta ggtgtgtctt
    36241 tatctgagaa acaacaatcc gatcatggaa cttttattga ttttgagaag gaacaatggg
    36301 tgatccaaca aaaggataaa tctggtatac gccgctatac gatgaattat agcccatctt
    36361 tccaaccaga ttctgtacgt acgcccgaag atttccaacg atttttagaa agagaatttt
    36421 atgccagtaa tcgtccaaca atcattttat cttatagttt aagtcttggt cttgtcctat
    36481 ttgtcatgac gagtctcatt ttattcgggg cttccttttt cttgtggatg acaagaaaaa
    36541 gccaactttc atccattcat acattcaaag agagtgcaaa tttgatgctg aatgttatgg
    36601 gaataggttc catggcagca gcagttgtag ggtttatcca ctttgacttt atcttgatgc
    36661 taagtgtcca aacaacgata actgtcttgc tgcttttatg ggtttttgtc aaaacaagat
    36721 tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa
    36781 aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt
    36841 cttttctttt ttagtattaa cgtaataggg tagttttttt aaggaggaaa atcatgttag
    36901 agaaaacttt atacaatcaa ttattttcac gttctttctc gctccctgtc gaagtaaatt
    36961 attgggacgg tacaacgaaa caatacggag atacggacac tcctccccaa atcaaaatca
    37021 atatccatga agaaatccca atgaaagaga ttacaagtaa cgcttctttg gcactaggag
    37081 aagcctatat ggatcatcga atcgagatcg agggaagtat ccaagcattg atcaacgatg
    37141 cttatcagca agcaaacagc tttttacgag gaaaagatta tctaaaatgg ctgccaaaga
    37201 aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca tgcgcattat gacttaggta
    37261 acgactttta taaactttgg ctagatccaa ctttgactta ttcttgtgct tatttcaata
    37321 cacctgaaga tacgttggaa caagcacaaa tcaataaagt ccatcatatc ttagacaaac
    37381 tctttatcaa agaaggcgaa acgttgcttg atatcggttg tggctgggga acattgatgt
    37441 tcactgctac caaagagtat aatgtcaagg caactgggat cacactaagt gaagaacaat
    37501 acgatttgat taccaaaaga ataaaagaag aacatttaga agataaatgc cgtgtattat
    37561 taatggatta ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt
    37621 tcgaacatgt aggttctgaa aatcttgaag gttatttcaa agtggtaaaa gatttattga
    37681 aaccaaaagg aacagctttg atccatggaa tcagccgaca acaaggcggt gcgacaaatg
    37741 cttggatcaa taaatatatt ttccctggcg gttatgtccc tggtgttact gaattagttg
    37801 ataatattac aaaaaacgat ttgcaactga ttgatttgga aagcttacgt cgtgactacc
    37861 aattgacatt agaacattgg acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag
    37921 aaatgggtga ttccttctat cgaatgtggg atctatattt acaagcttgt gcagcttcat
    37981 ttgaatcaag taatatcgat gtgattcaat atttgcttgt tcaacctgga aacaatgaca
    38041 tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt aaagactatt taaaaagaag
    38101 ctaagtttta tgatgctcca gaagtgaact tttttgtcta acttctagga ttgtctctaa
    38161 agcttagttt ctttttcttt atattttcat aataaaagtt tggttcttta atttttaaac
    38221 atgacagaaa attttttctt cgttatactt gtaatagaat actattttat aacaaagggg
    38281 atcgaatcaa tgaaaaaaac tgttatttat actagtttat ttgcagcttt gtttttagct
    38341 gcttgtcaat caaataccaa tcaaggcaca tctgggaata cgtcttccag tagcgaacag
    38401 cgctcttcac aggtatcgga ctcctcaaca aaagcaagca catcttcgtc tagccagaca
    38461 aaaagttcta gtgaagcaag tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc
    38521 gaattgttcc aagataaata cccagatact gcaattacta gtttggaatt agatagtgat
    38581 tggggatctt acttctacaa gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga
    38641 attgatgctc aaaatgaaaa aatggagaca gagaatccag aaactctaga tcgagatgaa
    38701 caaaatggtc aaaagaaaaa agaagatggt ctggatgttg ataaattgat cacgattgag
    38761 gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta cggcaacaga ttgggatttg
    38821 gataaagatt taggtactac ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca
    38881 tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa
    38941 tttcttcgga aaacaagagt tgatttttct tttagtcatg atacactaga caaaataact
    39001 tgtttgaagg agcgatttac ttgagtttag aaacgacctt tgccaatcat atctttgcca
    39061 atcctttgat gaatgcatct ggtgtccact gcatgactac ccaggaatta gatgaactgg
    39121 ctcattcaga agcaggagca tttatcacta agagctgtac gatcaatgaa cgaaaaggaa
    39181 atcccgaacc acgctatttt gatgttcctt tagggagtat caattctatg ggacttccaa
    39241 acttaggttt ttcttattat ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc
    39301 aaaatcaacc gttatttttt tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc
    39361 ttgagaaaat agaaaaaagt gggtttaatg ggatcactga attaaattta tcttgtccca
    39421 atgtgcctgg aaaaccacaa ctagcttacg attttgaggc cacttatgaa acactgaaag
    39481 aagtattcag catcttttct aagccgctag gaatcaaatt gcctccttat tttgattttg
    39541 ctcattttga tcagatggct gatatcttga atcagtttcc attgacctat gtcaatgcta
    39601 tcaacagtgt gggcaatggt ttgtatatcg atacagaaca agaagcagta gtcatcaaac
    39661 caaaagaagg gttcggcggc attggcggag agtatatcaa gccgactgct ttggcgaatg
    39721 ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat cattggtaca ggtgggattc
    39781 ggacaggaca agatgcattt gaacatctgc tatgtggcgc aagcatgtta cagattggta
    39841 ccgagcttca taaagaaggg ccggaaattt tttctagaat cataaaagaa ttaacgcaga
    39901 ttatgtcaga aaaaggatat acgtcaattg acgaatttaa aggcaaatta cgcaccattt
    39961 catagcaaag agacgaaggt catgattgat cttcgtcttt ttttataaaa aacactcgtc
    40021 tgttcattcc tgtgcaagcg tactcattgt atgttagacg aacttgtggg aaaataacag
    40081 aggcaaaaag gaggataata tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa
    40141 agaatttagt tttcaagaac tagttacgga tatacagaaa aaaataaaga agcaaaatca
    40201 tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt aattcccacg agctattgat
    40261 tccccagtca gaaaaagggc cattggctgg tatcccattt cctttaaaaa tgctcggtca
    40321 agaaaaaaag ggatggctag ctacttcagg atctcgttta tttgaaacac accgagcttc
    40381 tcatacatct aattatgtgc ggcaagcaga agcgataggt ctagttcctt ttggacaaac
    40441 caatgcgcca gagtttggtt tcaaaaatat tacagaccca gtcatctatg gcccagcaag
    40501 aaacccttgg aaccttgaat gtacaccggg cggatcaagt ggaggagctg ctgctgcagt
    40561 ttcatcagga atcgttcctt tagctggtgc tagtgatggc ggtggttcta ttagaatacc
    40621 cgcttcgttt tgcggattga ttgggctgaa accttcgcga gggacgatgc cagttggtcc
    40681 atatgcttgg agaggatggc aaggagctgc tattgatttc ggcttgacag tttctatgag
    40741 agacacggag gctttatttt atgggatgag aagtatccat tccggtgcgc catatcaagt
    40801 atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt
    40861 tgattcaccg atccactcac agattagtga agaggcagta cttgctgtaa aaaaagctgt
    40921 agatttcctt ggaaaagaag ggcatgattt gacagagatt ccttacccac tagatggtcg
    40981 taaactgatc acgtcttatt atcagatgaa tggcgcagaa acagcagcaa tgatccgctc
    41041 gattgaaaca ggcatccagc gagctgtcag aaaagaagaa cttgaaccca tttcttggac
    41101 tttgttccaa tatggtcaga agctctctgc cgctacttac atcgattctc tgcatgtatg
    41161 ggatgaagca gcaattgtca tggaaaacct acatcaagaa tacgatctat tcctttcacc
    41221 aacgactgca tttaccgccc ctaaaatcag cgaagatttg caaagtgatg cgataagagc
    41281 acagatggaa cacatcagtg attgttctga agtagaaggt ttagacgttg tttcttccat
    41341 gtttgaggag agcttgaaga tcacacctta tacacagttg gctaatctga ctggacaacc
    41401 agcaatcagc ttgcctactc atttgacaaa agaaaacatg cctctaggaa tccaatttat
    41461 ggctgccaga ggaagagaag atttgctatt tcaaatcggc tatctatttg aagcaaatca
    41521 tcagttccat ttaccaaatc catggaaata gcagaaacaa ggagaagaag aatgaagaaa
    41581 aaaagattgt ttgctctaat aactagtgcc ttttttattt taagcgcttg cggtacagct
    41641 gccgctaatc aggaagataa cagctggagt aatgtagaaa aaaagaaaga attagtagta
    41701 gcgacttctg gcacactgtt tcccgcatct tattataatg atcaaaatga attagttggt
    41761 tatgatgtag atttggcaaa agaggtggct aaacggttaa atgtcaaaat ctcctttaaa
    41821 gaatacaacg tggatggaca aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg
    41881 aacgattttg gtatcacaaa agaaagagaa aaaaaattct ccctttcaga gactttcaaa
    41941 tattcatttg acagtatgat cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa
    42001 gatcttaaag ggaaaaaagc agctggcgaa cccaatacta gctatatgcg tttagcggaa
    42061 aaacacggag ctaaattagt cacatatgac aatgccacga atgatcagta tttaagcgat
    42121 gtagcaaatg gtcgaacaga tgttattcta aatgattatt atctgcagaa aatggccgta
    42181 tctgctttac cagatatccc tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa
    42241 agtggttttt tattgaaaga aggaaatgat gctctgaaaa agaaaatcga tggcgtatta
    42301 aaagagatga aagaagatgg gacgacaaag aaaatatctg aaaaatattt tcaaactgat
    42361 gtttcagtca aaccgaaaga aacgatggct gagtcggtga gtgagtgatg tactttcctc
    42421 taattgattt ttccctcatg tggacttcac ttcctttcgt actgcaaggc ttggtataca
    42481 cattagggat tggcttcgtg tcgtttcttt taggaaatct ggtagggttg cttttgacag
    42541 ttttaggtct tttggattgg ctgcctctga atgtatttat tcgtttttat ttatcgttct
    42601 ttagaggcat tcccgcacta gtcctgctgt ttctgttata ttttgggtta ccttatcaat
    42661 taagtgcctt aacggcttca gtcatctgct ttacgattac cagcagtgca tttattggtg
    42721 aaatctatcg aggatcacta gcaggcgttt cttcaggtca gtgggacgct ggtttagctt
    42781 ctggtttttc tttctttggg gtattgaaat atatcgtttt gccgcaagct tttcgaatct
    42841 cagtaccggc actcagtaac gtagctatgg atctagtgaa aggtacttcg ttagcagcaa
    42901 caatcacagt gccagaaata tttcaaaaag caaagatcat aggcggtcgg acattcgatt
    42961 atttttctct ctatatttta gttgcactga tttattgggt catttgtatc gggatcgaaa
    43021 aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc aatctgaaaa atagaaatgg
    43081 tgctagcgtg gctggcacca tttctatttt ttgtttcttg tatattctgc ctagtatttt
    43141 aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac
    43201 cccgctaacc atcgaaatag aatcctcgtt ataccaatat tgcatcgagc aaggcggctt
    43261 ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt gtagatacgc ttagctgctt
    43321 gacaaaattt gatggaagcc gagaatggcg atgctatgag ctgaaagtga caaaagcaga
    43381 ttttcgctct acagccaaac tgtcatttgt tggacattat aattattttg ttttaccaag
    43441 taaactatat gaagaagtaa aaactgaaat ccctacagga ataggtgttt tagtgtatcg
    43501 tccgtacacg gttgaagagg agatgcctgc ctctggtaca tttctgattg cgaaaaaagc
    43561 agcaaagaaa gagcttgctg taccggaaga agctttgaca aaccgcttca tggcttcttt
    43621 attcagggaa gtaagaaaag caaagcaaat ggagcgaggc gtaaaatatt ttccaacaga
    43681 tcaattgtac aaagaattga aaagacgttc agaaaatcaa gatccatttt ctaacgaacg
    43741 atattatgaa cggtttttag aggaaatagc tagtcaggca atcaatgagt tgcaagaaga
    43801 agttatcgcg cttcagcatg attatgaata tttgaggcaa caacagcaga tccgtcggtt
    43861 gcctacagag ccattggagt gaaaaatatg tactatcctt atttaagagg aaaacaattt
    43921 gacctattag cattaaaaga agcactgtcg cgaggattgc tctccaataa aatccaacca
    43981 gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa
    44041 aagcaccatc caatcgctgt gatccaaaat ccccaagtag gacaattcaa attattcgat
    44101 cagcatgtgc acagttggac agtcaaagaa aatagctctg tcgtccctgc acagatctta
    44161 acgccagaaa atctcagtga agtactggat tcacctccag cgtttcttgt tttcgatgga
    44221 cagcactacc caaaagatac agaggtgtgg aaacagttgg caggagttga ttcgaaattt
    44281 ttgataccag atactagtcg ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt
    44341 gactcttttc aaacgagaaa gcatgttgaa agttatgcag ataaaacaga tgactttttt
    44401 agtgatgact atcttttttt ccacgcagac ggatatattg gattttctga tttcacgatc
    44461 gaaggtagcc gttatttcga taaagggttc cctagccgag cgcttgctat ccatctaacg
    44521 tatattgatg cttacggcaa tatacgcgtg aaacattttg tctctgacag taacgactct
    44581 gctaaagacc aagcattgaa gtttcttgaa gcaggagata aaatgaaaaa atggatcatg
    44641 cgtcaccatc atcaactctt gattacttca ggaatgattg aattattggc tctttaccag
    44701 caacaaaaat tccctggact gggagtgttg aaaaagtggt cacttatgca tcatttggaa
    44761 cttattagtc acctgctaga tcatccaaaa gattggctta aatattattc aaaagttcag
    44821 gaactatatg aagtgatcca atgattagat aatgtaaatg aaaaaggaat agaaaagagg
    44881 aaactggatg tatattcggc aaacaaggaa agaagaagta gaagaagtgg tagaaatcat
    44941 taatgccgca gcggatttca ttgcttcgca aggctctcct caatggcaaa acgggcagaa
    45001 accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa gaaagctatg tattaatagt
    45061 ggaagatcaa attgctggaa cagctgcatt agtctcaagc gtggatcccg tttacacaaa
    45121 aatagatggt agttggaaag aatcaactgg tccttattta tcgattcatc gtgtcgcttt
    45181 agcagaaagt actcgaggaa aaaaactagc aaaaccgttc ttagaacatt tgttgacgat
    45241 tgcagccagc aaaaatatcc aagatatccg tatcgatact tatccagcta atgaaccgat
    45301 ggaaaagacc atctattctg ctggattcac ttatcaaggg atgattgaat ttccttttga
    45361 acacggcgaa agaaaagcat atcaaataat catcaattga ctataaaatc atctgagcaa
    45421 agataaagac attaacacta ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca
    45481 gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa atgcttctca aatttttgtg
    45541 gaattttagc gtattttacg aaatagctgc tttctgaaac tcccttcatc gagaggtcgt
    45601 gacatagctt ttgtcacgac ctctcggctt atcctacgag tattattcga tacatttata
    45661 tcaataatcc tttttttatt ttatctgcag cttcttggaa cgctccatcg tttgacacga
    45721 ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc taaatcttct acataaaccg
    45781 ccagtctggc tgaaatctgt tcagatgaat ctccacgttt tttcaggcgc gattgcaatg
    45841 tttctttaga gatcgtaata aagataggac ggactaccgt cccgaaatac tcatataaat
    45901 gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc tactgtcagt ttctttttaa
    45961 tttctgcttt actggaacca tatttatgtt gagcatattg gtcccactct gcaaactcat
    46021 ttcccttgat catctgattg aatgtattgt cagaaacaaa ataataatcc tcttgatctt
    46081 tttccccagg tcttggtgat cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa
    46141 tagcttctgc gagtgaagtt tttccagaac cagaaggacc gataaataca tagcaataat
    46201 tcattgtcat ttctcctttg atgattgaac aagtgaaaaa gctttggaaa atatttccaa
    46261 agcttttaaa agtaactttg cttttttgta tattatacca gcaagccagt cacaatagca
    46321 cttaaaaagc tgactaaagt cgctgttagc aaaattttca atccgtgttt tgcgacaaga
    46381 tttccttttt cttcatttaa tcccttcatc gcaccagaaa tgatcccgat agaagagaag
    46441 ttggcaaatg aaacgaggaa gacagatata attgctgttg tacgctcagt aaaatggaaa
    46501 ttaccatttg ctaattctgt catagcaaca aattcatttg taaccagttt agtcgccatg
    46561 atactaccgg catccactgc ttctttccat gggattccgc tgatgaaggc aagaggggca
    46621 aagatgtaac ctaggatctc ttggaaagtg atgccgaata tcccgtggaa gatagcattg
    46681 atcatggcaa tcaaagcaac aaaaccgata agcatagcag caaccgtgat cgccacatgg
    46741 aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg tctgttcttt gttttcttca
    46801 atgatcagct catcttcttt ttcttctaat acatagggat ttacaattga agccagaata
    46861 taaccgccaa ataagttcaa aacaagggca gtgatcacat atttagaatc gatcagtgcc
    46921 atataagatc cgacgatcga catagaaact gtagacatag ccgatacact catggtgaac
    46981 aaacgtttct cagataggaa gggtaattcc ttttttatgg aaataaaaac ttccgattgt
    47041 cctaaaatag cagaagctac tccattataa gattccaatt tgcccatccc gttgatttta
    47101 cttaatccta gaccaacagc tttcataaat aatggaagaa tcttgatata gcgtagaata
    47161 ccgataatag cagaaataaa tacaattggc atcaatacgg aaagaaagaa aggtgcactt
    47221 cctttatttg ctacaccggc aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga
    47281 tgatcaaaca tttttgccaa tacagaaaca accgctgtac caaaagttgt acgtaataat
    47341 acgaaaccaa gaacaagctg taaaacgaac atcacaatta gtggtttcaa tttgattgct
    47401 tttttgttac tgctgatcaa aaaagcaagc cctaatgtga caaataaacc aataatacct
    47461 aataaataac tcaaataatc actcctattc caaattccgt tttattttac ctgttttgca
    47521 aataaaatac taatctttag tgaaattttc attacttttt tagagtgaaa cgcattcatt
    47581 ttccattaga aaataaagcg aacaaaataa aaggtttaaa aagtgtaacg tcttagtaaa
    47641 gtctataaaa aaacgaaact atcgattaag ctaatagttt cgttttcaac atttataagt
    47701 aaactttttc tattggtaat atagattttc ggaagggtag acaggtgcgc aggtaatatc
    47761 gattcctagt tttttcaatg tttgttcatc atttttattg accatcacag tagagtgggc
    47821 ttgtgctccc tcaagttcag ctagtttatc ataagcaact tgagcgattg gattcgttac
    47881 tgcactgatt gataaagcga tcaagacttc attgatgctt agtgcatcca aagaggaatg
    47941 aagtgtcttt gatttcaatc cttgtattgt ttcaagaact aatggtgaca ataaaggaat
    48001 gtcatctgcg atatgggcta aatgttttac tgcatttaag ataactgccg ctgacgcatc
    48061 catcaatgaa cttgtacgcc cggtaatgat gacttcatct ggtaattcga ttgcaatcac
    48121 agcagaagga tcttttgtag gactttgcgc ttgaatctta tctgcatagt catgggcagg
    48181 gactaccacg tttcggtctt caggacgtaa accgacttct tccataatca gtttgattcg
    48241 attaactgct tcttcgtcga tcaatccttt tttgaagtcg cattctgttt ggaaataacg
    48301 acggatgatt tcttgtttag aggcttcttg gacaacggta tcatccacga tcccaaatcc
    48361 tacacgattc acacccatgt cagtaggaga ttgataaaca gattctttac cagtaatttt
    48421 ttcaataata cgtttgatta caggaaatgt ctcaacatcc cgattatagt tcacagctac
    48481 tttattgtag gcatcaaagt ggaaggaatc gatcatattg acatctttta gatcgactgt
    48541 ggctgcttca taagcaatgt tcagcggatg tttcaacgga acattccata ctgggaaagt
    48601 ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt tcatgataca gttggttcaa
    48661 ggaagtagcg agctttccac ttcctgggcc aggagctgtc actacaacga ttggtttagt
    48721 tgtttcaata tactcatttt ttccaaaacc atcttctgag acgatttttt ctacgttgac
    48781 tggatagtct tcgatttctg agtgtttata gaccttgatg tttcgtcttt ctagtttatt
    48841 gataaagact ttcgtggcag gctgtccact atagcgagtg ataacgacac tgttgattaa
    48901 taagccataa ccacgtaatt catcgattaa ccgcaagata tccatatcat aagtaatccc
    48961 ataatcacca cggatcttat tacgttcaat atctccagca tagacgcaaa tcagtatttc
    49021 agcttgatct ttcaattttt gcagcaattt gattttcgca tcttcttcaa atcctggaag
    49081 tacacgtttc gcatgcttgt cgtcaactag cttcccacca aattctaaat ataatttgtc
    49141 atagtggttc actcgttcca aaatatacgc tgactgctct tcaatatatt tttctgaatc
    49201 aaaaccaatt tttttcacta ggttccctcc ttaagtacta aagcattttt tcattttttt
    49261 gaggattcgt caactggaag agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc
    49321 aacttgtcta gatagttatt cattggtatg atagtttttg ataaggaagg tgctacatga
    49381 aaaaaagaca gaaaaagaaa aacgcatata aacactatat ccgttcgatt tttaccggtt
    49441 atgaaagaat gcttgaagat cccgaattgg aacaattgac gtttacttat ctaaacgaag
    49501 aaacgcagct aactcgtgat gagcatcagc gcattcattt cacaacaaga gatctgccta
    49561 gtaaataaaa aataagataa aggtcatcgg attcgattct gtcctatcag atggaaagac
    49621 tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag cctgttttat ggagcattgc
    49681 ccgctctttt ttataacaat tttttacaga aaatggtact attgaagtat taataagtga
    49741 aaaacagatg agtaagagga ggcaagtagt atgaaagagc cagatacaaa tgcaaattat
    49801 gagccagacg aactagagga gacgaatgcg gatcaggata caggacaaga atcgtcttct
    49861 ttttacgata tcaaagaaaa agcaagcatg aatctttccc aattattaga agacttacag
    49921 gcttttgaaa aagctgtccg cgaagaagat atccctgaga tctaccgttt atacaatggc
    49981 cgattgaatg aagaattaaa aagaagctca aatgaaaatc acgagatcga tcaattgctg
    50041 atgcgtaaga tccatgatcg ttttttacag gaatttcctt ttatggaaca agtagaaaac
    50101 atctcgccaa cgatgagcta ttacaaaatc ggcacttatt accatgatcg gcccacggtc
    50161 ggtattgatg caagtctgcc agaaattttt gttttgccta agatcgatga agagtgggag
    50221 aaatattccc aaccggagtc aacggactat gacaaaaaaa tcaatgagct agacgcaaag
    50281 gtgatcactg ctcagacaga aattgaacgt ttagatgaac aaataaaaga aatcaatcga
    50341 caaatgactg atttgaacga caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa
    50401 attcaagaat tagagaagaa gaagcaagta ctatctaatg aaaaattagg ctggcttcct
    50461 tatattgaga cgcctgaaac aatccagcag caaaaagaag cattgaagca agaagcaaga
    50521 gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag acgctatttc
    50581 ggtagcaaag agggattcgg tcaagcaatc catgaattct tgatgaatta tcttggcaat
    50641 gagaatccta ataatcaatc taatgaagga ggttccgagt atgaatgaac acgatgaagc
    50701 agaatggaca aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca cttcattgga
    50761 agaacaaaca agtaatcctc tggatgacac agaagaaatc cctatgctag aggaaacaaa
    50821 aatacacgaa aaagaaaaag taactccgac gaatacgaat actgcagaaa acacccatgc
    50881 ttacgaaata actgaaaatc ccgggtattt agaaggaaaa gagcaagatc atcaagatat
    50941 cgtcagtttg attgaaacag cggaagcaga attagcggat ttacgtatga gaaaagattt
    51001 gatcgaatca gattttccac aattattaga atcatatgat cgttttatat tgaatgaaga
    51061 accaatctcc acgatcggaa agacaagcct gatcgaatac ttcacgtatg aattactgaa
    51121 accgttgaat gatattggtc tggaacggtc aaattcagct tatgacacat ggaaaacaag
    51181 acatttttcg atcaattata cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt
    51241 accgaccatc gatcaacgat accaagtgga atcgatgagt ctcctaaacg tatcaccaga
    51301 gacaatggaa atcaatgtcc aagatgaccg ggtgctttct ttgatccgac actggtcagt
    51361 ggatcgagta ttctctgcag ggcagatcac gatattcaat cacaaattga atcaattatt
    51421 agcccatgca agaacacttg gttttaccgt taatcagaca ttactagaca atacgaagcc
    51481 acttcgtttg aatcttcaga gcgaatttga attgacgaat gaagtattgg atgatatttt
    51541 tatcgtggca atgaaaaatc catcctacga tatggaaaag atcaacgaaa ctacttatca
    51601 agtacgatta gataaaggac agtctttaac gataacaaca aacgaaaaag atcaaacagt
    51661 cttgtcgatc tcatctggag actacccacg atctgtcatt gatttcttta tcaattacga
    51721 attcttggtc ccattaatgg tgagaaagtc ctgattggat aagttggatt accagatgtc
    51781 attcagataa ttctgttcca aaggacttgc ggcagttatc cagaataatt gtcacaagtc
    51841 ttttctgtat gtactaaatg gacgtgaata acttgctgat gaaggaggga tttcattagg
    51901 aatgtccaca aataaaatag cacagcttag cttattgtct gctgcttgtg tagcaggtag
    51961 gatcgctttt caatttattc cgaatttcca gccaatgact gctatttttt tatttatagt
    52021 actttactta agcttaaaag atgcgttaat tgtcatgagt ctttctattg cgatcagcag
    52081 tttttatttt ggtgttggac catgggtcat cggccaatgg atcagctata ctgttgttct
    52141 gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg
    52201 agtctgtttt tttctggcgg gcatggttta cggaataggt atgaccgttt ttgatacgct
    52261 gttatatcgt ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat
    52321 gcacagtata ggcaatgttg tgttctttct tgtttttctg ccagtcgtca aacgttttta
    52381 caatcattca ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca atgagtgtca
    52441 gtatactcat tttgacaagt tgcagtacaa cctatacaca atcggcttcc aaccaaaagg
    52501 aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt caaaactttt
    52561 cggaaaaaga agtgttcttc aaaaaaggcg atgatttact gagtattttg aaacgaaatt
    52621 ttgaagtcaa agaagataac ggcttcatca cttcgcttga agggcactca caggatgaga
    52681 aaaatcaact ctattggatg tttactgtgg acgggaaaag tgctactact ggtgccaaag
    52741 agatcaaatt gaatgatgga caatcggtca ttttcaattt gtctaaacta taagtttcat
    52801 aaaaaactgt taaagggcga atattgccat aattcagtga gttcagttga tgaaagaagg
    52861 aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct gctctagggg
    52921 ctaatattct agttgctatc agcaaatttg tcggctatgc aatcagtggc agtgccgcca
    52981 tgctaaacga aagcatccac agtatcgttg attgcagcaa tcagattttg ctgttattcg
    53041 gtgataaaca agccaataaa ggacagagcg agttgcatca atttggtgaa ggacgagcaa
    53101 aatacttttt cagtacaatc gtcgcaatga tgcttttctt cggcggtggg gcacttgggg
    53161 tgatggaagc ttttgaaaag ttgacgcatc catcccatga agtgggtaat ccgatcattg
    53221 ttatagcaat actgttattt ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga
    53281 aagaaatcaa agaattgaat actgaaaaac tttccacgat gcgctttttg cgcgaaagtc
    53341 gacacagtga aatactgatt atttttacgg aagacttgtg tgctgtaatc ggtttggtct
    53401 tggctttagc aggaacaata ttgacttatg caaccgggat tgctgcattt gatgcgatca
    53461 gcggattgct gatcggtttc ttgctgatgg gagccgccat tttcctagca aaagaatttt
    53521 atagtctgat catcggtgaa agcgtgacag caagcgattt atcaaaaatc aagttagctt
    53581 ttgaacgtcc tgagatcagt cggctgatcg atataaaaac agttcattta agtccaacgg
    53641 agattttagt tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa gcttactcgg
    53701 tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag atgtatatat
    53761 acattgaaac agataaatat gacccaaatt attctcgtaa ataaaacgtt tattcatgaa
    53821 ttgaagcaaa aatgagaaaa atataatctt gtttttaaat aaaacggcta caatttagcg
    53881 aatttatgtt acaatgagtc cgctgtttat ttgttgtaat taaaggagat attttaatga
    53941 acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc agtgatgcca ctttgtataa
    54001 gctatatacc agtaggatta gcttgcggag tattgttgca aaaagttgga tttgatcctc
    54061 tcttatcagg actgttatcg attctggtct tttccggtgg cgcgcagttt ttagttgcat
    54121 ctatgcttac gacgcaagca tcatttgcaa cgacattatt gatggttttc tttttagaat
    54181 tacgctatac attattagga gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt
    54241 ttttggctgt cttttctcaa tcactgaatg atgaaaacta tgcggtgaac tacttaaagt
    54301 tttcaactga cccttcctgg aataaaagaa aggctttata tgtcaattgg ttctcaatga
    54361 ctgcatgggc cggtagcaat atgatcggta attttttcgg ctcggtgatt cgagtagatg
    54421 ctgaccttgt ccattttgct ttgacggcca tgttcatttt catgtttatc atgcagatga
    54481 aaagcgcact actgatcttt acaggtttat tctcaggcgt attaggggtc gtattcatgg
    54541 tattattcca aaatactttc ggattgattg cagcaacagt cattgcttca tttgccggat
    54601 ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa actaagagac cgcggtaaag
    54661 atgtcaatga gccgctcttc aacttcccgg aacaggaggc caatcaccat gactaaacta
    54721 tatttgctac ttttagttgc ttgtctgttc atcgtggcat atatcccgcg cttgttcccg
    54781 atgctttatt ttactcatcg aaaagtgcca tcctggttca gtgactggat gaaatatgta
    54841 ccggtggcat tatttgcggc gttagctttt aaagatgttt tcattactca tgaacatctg
    54901 gatattgctt ggaatatcaa aatagctgca atgattctag tagcaggcgt ggcttacaaa
    54961 acacgttcga tggcgttgtc agttttgaca ggacttgcgt ctgtcttttt gctgtctatg
    55021 ctttaaactg ttttatttta ttaaaaacag agattgcgac ataagttgtt caaactgatc
    55081 tatccgaact gcatttgcaa cagtcaattc tggatgcaag tttattcgta gggataaaaa
    55141 agattagtgt tggcttatga tgcaagctct tttttatttt aggaattaga gagggatttg
    55201 tccttctatg attttttgct ataataggga agatgtttat tttatgaaag aatagttgag
    55261 ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt ggctaacctt tgctaatctt
    55321 ctatgtaaag ttttaggggt agtctatttg attccctggc taagtatgat ggggaacaat
    55381 caagatggta tgcttgctac gacattgtat aatgttggct atcttcctta tggattgttc
    55441 ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa aaaaagtagc tgtagccaca
    55501 aaagaaggag ataacgctgc ttgtcggctg atttttagaa gtacgatcaa tatcatgttt
    55561 gtcatcggta tcgcctctgc tgcactgatg tatttatttg ctcctttact agccggaatc
    55621 agtccgattg ctaacgttaa taatgggata ctagctatca gaagtttatg cccatctttg
    55681 attgctatcc caattcttag tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg
    55741 ccttatggaa cttctttgat tatcgaacag gctgtgcgag tgatcgttat cttggctggt
    55801 acttattact tgcgcgtatt gactgacgga accatattag aagctgtatt gatcagtaca
    55861 gtcgcttctt tcttcggtgg gttagctgcg attatccata tgtatatcgt cggacgcaga
    55921 aaagacttct tcgaacttaa agattttttg atttccagcc gttatttcga aagagaaaac
    55981 cgatctgcct ctgtctctat cattcgggaa acactaccgt ttatttttgt cggctcagtc
    56041 attactatcg ttcaaatgat cgaccaagta acgatgaaac cactgcttca ctttttccgt
    56101 ccagagatcg cagatcagca actggaattc ttattcagcc gtgcttctgt caatccaaat
    56161 aaattgacat tgatcctgat ctccatggta ggaacagtag ctataagcag cttgccaata
    56221 ttaagcacga tgaagaaaaa agatcggata caaatcgaaa aaacagtagg agatagtttt
    56281 tcgattgcgt tattgattct tcttccttca ttgactggaa tgtctttatt agctggacca
    56341 ctatacacat tgttttttgg ctatgatcct gaaagtgtcg gatatttcca aatggctttg
    56401 ctagcttctt tgtttttctc tttattcacg atcctatcta cgatgcttca atctttgaat
    56461 caccatcgtt ttgcgatccg attaactgta gaagcaatta tcctgaaagt catcttccaa
    56521 gtaatcggat taggattagt tggcggatac gggatgagct tatcaaatgg cttagcattc
    56581 ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag aataccgaat agcaccttta
    56641 gcaaagatca gcaatttttt cttaaaaact ttccgctcca ccctgattat gcttgcagta
    56701 tgttttgccg ttttcttttt gctgaatcag cgattgtcaa tggaatcaaa aagctatgca
    56761 atgatttatt gtgtggctgt aggaagtatt ggcggcttag tatttttatt ttctcaattc
    56821 ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc atagcagaag caaagaatag
    56881 gttaactaat cgttttttgc tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc
    56941 gtttttctat tttccaattt tagtttcata tctaatctta cttatgatat agttataagg
    57001 tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga aacttgcagt agttacagat
    57061 agttcggcat atctgccgga acgtatcaag aaccatcccg acttattcgt tattccaatc
    57121 cctttgatta tggatgggaa aatttataac gaaggaatcg atatcgaagc ggatgaatat
    57181 tatggacttt taaaaaacag taaagatttt cctactactt cacaacctgt cgtaggggaa
    57241 gttttgggtt tatatgagga attgaaagag aaagggtatg acacgatttt aagtatccat
    57301 ctttcttcag ggatttctgg ctttgtcaac acattatttg cgatgaaaga tgacatcagc
    57361 ggagtaacgg tcattcctta tgactctaag atcactagta tgccgatggg acacatggta
    57421 gaagctgcct tagacttaaa tgatgaaggt cagtcccttg atgaaatact tgcacatatc
    57481 gatcgaatcc gagataatac ctatgcttat ctgattgtag atgatttgaa caatttagta
    57541 cgaggaggac gtttgacaaa tggtgcagcg ctgattggca gcttattaaa aatcaaaccg
    57601 atcctgacat tttctgaagg gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa
    57661 gcatttgctc gagctgaaga aattattggt agaagaaata acgaaataca accgccagtc
    57721 aaactttacg tgatacatgc aaataattta gaagtagcga ttgcggagaa agagaaattg
    57781 cagaaaaaat atccaaatgc agaaatcgaa attggtcatt tcggaccagt cattggtaca
    57841 catt
//
LOCUS       DASOUD010000006        53916 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.006, whole genome shotgun sequence.
ACCESSION   DASOUD010000006 DASOUD010000000
VERSION     DASOUD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.006"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            273..389
                     /locus_tag="U4A29_000359"
     CDS             273..389
                     /locus_tag="U4A29_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO0679509.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            1317..1655
                     /locus_tag="U4A29_000360"
     CDS             1317..1655
                     /locus_tag="U4A29_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679510.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            1749..1979
                     /locus_tag="U4A29_000361"
     CDS             1749..1979
                     /locus_tag="U4A29_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679511.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            2379..2750
                     /locus_tag="U4A29_000362"
     CDS             2379..2750
                     /locus_tag="U4A29_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEO0679512.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(3024..3716)
                     /locus_tag="U4A29_000363"
     CDS             complement(3024..3716)
                     /locus_tag="U4A29_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO0679513.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(3719..4504)
                     /locus_tag="U4A29_000364"
     CDS             complement(3719..4504)
                     /locus_tag="U4A29_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO0679514.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            4741..6141
                     /gene="lacG"
                     /locus_tag="U4A29_000365"
     CDS             4741..6141
                     /gene="lacG"
                     /locus_tag="U4A29_000365"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEO0679515.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6166..6483
                     /locus_tag="U4A29_000366"
     CDS             6166..6483
                     /locus_tag="U4A29_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO0679516.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            6496..8169
                     /locus_tag="U4A29_000367"
     CDS             6496..8169
                     /locus_tag="U4A29_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEO0679517.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            8183..9814
                     /locus_tag="U4A29_000368"
     CDS             8183..9814
                     /locus_tag="U4A29_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEO0679518.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(10305..11870)
                     /gene="guaA"
                     /locus_tag="U4A29_000369"
     CDS             complement(10305..11870)
                     /gene="guaA"
                     /locus_tag="U4A29_000369"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO0679519.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(11929..12852)
                     /locus_tag="U4A29_000370"
     CDS             complement(11929..12852)
                     /locus_tag="U4A29_000370"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO0679520.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13048..13653
                     /locus_tag="U4A29_000371"
     CDS             13048..13653
                     /locus_tag="U4A29_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO0679521.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            13961..14977
                     /locus_tag="U4A29_000372"
     CDS             13961..14977
                     /locus_tag="U4A29_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO0679522.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            15118..16419
                     /locus_tag="U4A29_000373"
     CDS             15118..16419
                     /locus_tag="U4A29_000373"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEO0679523.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            16465..17127
                     /gene="deoC"
                     /locus_tag="U4A29_000374"
     CDS             16465..17127
                     /gene="deoC"
                     /locus_tag="U4A29_000374"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO0679524.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            17148..17546
                     /locus_tag="U4A29_000375"
     CDS             17148..17546
                     /locus_tag="U4A29_000375"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO0679525.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            17612..18694
                     /locus_tag="U4A29_000376"
     CDS             17612..18694
                     /locus_tag="U4A29_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO0679526.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            18906..20468
                     /locus_tag="U4A29_000377"
     CDS             18906..20468
                     /locus_tag="U4A29_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0679527.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            20461..21594
                     /locus_tag="U4A29_000378"
     CDS             20461..21594
                     /locus_tag="U4A29_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0679528.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            21594..22550
                     /locus_tag="U4A29_000379"
     CDS             21594..22550
                     /locus_tag="U4A29_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0679529.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            22649..23815
                     /gene="deoB"
                     /locus_tag="U4A29_000380"
     CDS             22649..23815
                     /gene="deoB"
                     /locus_tag="U4A29_000380"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO0679530.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            23839..24657
                     /locus_tag="U4A29_000381"
     CDS             23839..24657
                     /locus_tag="U4A29_000381"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO0679531.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            24674..25381
                     /gene="deoD"
                     /locus_tag="U4A29_000382"
     CDS             24674..25381
                     /gene="deoD"
                     /locus_tag="U4A29_000382"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO0679532.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(25679..26365)
                     /gene="gpmA"
                     /locus_tag="U4A29_000383"
     CDS             complement(25679..26365)
                     /gene="gpmA"
                     /locus_tag="U4A29_000383"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEO0679533.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(26537..27118)
                     /locus_tag="U4A29_000384"
     CDS             complement(26537..27118)
                     /locus_tag="U4A29_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0679534.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(27251..27934)
                     /gene="rpiA"
                     /locus_tag="U4A29_000385"
     CDS             complement(27251..27934)
                     /gene="rpiA"
                     /locus_tag="U4A29_000385"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO0679535.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            28253..28666
                     /gene="rpsL"
                     /locus_tag="U4A29_000386"
     CDS             28253..28666
                     /gene="rpsL"
                     /locus_tag="U4A29_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO0679536.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            28750..29220
                     /gene="rpsG"
                     /locus_tag="U4A29_000387"
     CDS             28750..29220
                     /gene="rpsG"
                     /locus_tag="U4A29_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO0679537.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            29314..31401
                     /gene="fusA"
                     /locus_tag="U4A29_000388"
     CDS             29314..31401
                     /gene="fusA"
                     /locus_tag="U4A29_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO0679538.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            31573..32760
                     /gene="tuf"
                     /locus_tag="U4A29_000389"
     CDS             31573..32760
                     /gene="tuf"
                     /locus_tag="U4A29_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO0679539.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            33166..33474
                     /gene="rpsJ"
                     /locus_tag="U4A29_000390"
     CDS             33166..33474
                     /gene="rpsJ"
                     /locus_tag="U4A29_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO0679540.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            33505..34134
                     /gene="rplC"
                     /locus_tag="U4A29_000391"
     CDS             33505..34134
                     /gene="rplC"
                     /locus_tag="U4A29_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO0679541.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            34162..34785
                     /gene="rplD"
                     /locus_tag="U4A29_000392"
     CDS             34162..34785
                     /gene="rplD"
                     /locus_tag="U4A29_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO0679542.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            34785..35075
                     /gene="rplW"
                     /locus_tag="U4A29_000393"
     CDS             34785..35075
                     /gene="rplW"
                     /locus_tag="U4A29_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO0679543.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            35114..35947
                     /gene="rplB"
                     /locus_tag="U4A29_000394"
     CDS             35114..35947
                     /gene="rplB"
                     /locus_tag="U4A29_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO0679544.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            35990..36268
                     /gene="rpsS"
                     /locus_tag="U4A29_000395"
     CDS             35990..36268
                     /gene="rpsS"
                     /locus_tag="U4A29_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO0679545.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            36291..36638
                     /gene="rplV"
                     /locus_tag="U4A29_000396"
     CDS             36291..36638
                     /gene="rplV"
                     /locus_tag="U4A29_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO0679546.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            36652..37308
                     /gene="rpsC"
                     /locus_tag="U4A29_000397"
     CDS             36652..37308
                     /gene="rpsC"
                     /locus_tag="U4A29_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO0679547.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            37311..37745
                     /gene="rplP"
                     /locus_tag="U4A29_000398"
     CDS             37311..37745
                     /gene="rplP"
                     /locus_tag="U4A29_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO0679548.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            37735..37923
                     /gene="rpmC"
                     /locus_tag="U4A29_000399"
     CDS             37735..37923
                     /gene="rpmC"
                     /locus_tag="U4A29_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO0679549.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            37948..38214
                     /gene="rpsQ"
                     /locus_tag="U4A29_000400"
     CDS             37948..38214
                     /gene="rpsQ"
                     /locus_tag="U4A29_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO0679550.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            38272..38640
                     /gene="rplN"
                     /locus_tag="U4A29_000401"
     CDS             38272..38640
                     /gene="rplN"
                     /locus_tag="U4A29_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO0679551.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            38677..38985
                     /gene="rplX"
                     /locus_tag="U4A29_000402"
     CDS             38677..38985
                     /gene="rplX"
                     /locus_tag="U4A29_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO0679552.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            39013..39552
                     /gene="rplE"
                     /locus_tag="U4A29_000403"
     CDS             39013..39552
                     /gene="rplE"
                     /locus_tag="U4A29_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO0679553.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            39571..39756
                     /locus_tag="U4A29_000404"
     CDS             39571..39756
                     /locus_tag="U4A29_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO0679554.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            39792..40190
                     /gene="rpsH"
                     /locus_tag="U4A29_000405"
     CDS             39792..40190
                     /gene="rpsH"
                     /locus_tag="U4A29_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO0679555.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            40222..40758
                     /gene="rplF"
                     /locus_tag="U4A29_000406"
     CDS             40222..40758
                     /gene="rplF"
                     /locus_tag="U4A29_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO0679556.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            40919..41275
                     /gene="rplR"
                     /locus_tag="U4A29_000407"
     CDS             40919..41275
                     /gene="rplR"
                     /locus_tag="U4A29_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO0679557.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            41296..41796
                     /gene="rpsE"
                     /locus_tag="U4A29_000408"
     CDS             41296..41796
                     /gene="rpsE"
                     /locus_tag="U4A29_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO0679558.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            41811..41990
                     /gene="rpmD"
                     /locus_tag="U4A29_000409"
     CDS             41811..41990
                     /gene="rpmD"
                     /locus_tag="U4A29_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO0679559.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            42034..42474
                     /gene="rplO"
                     /locus_tag="U4A29_000410"
     CDS             42034..42474
                     /gene="rplO"
                     /locus_tag="U4A29_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO0679560.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            42474..43769
                     /gene="secY"
                     /locus_tag="U4A29_000411"
     CDS             42474..43769
                     /gene="secY"
                     /locus_tag="U4A29_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO0679561.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            43831..44478
                     /locus_tag="U4A29_000412"
     CDS             43831..44478
                     /locus_tag="U4A29_000412"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO0679562.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            44669..44887
                     /gene="infA"
                     /locus_tag="U4A29_000413"
     CDS             44669..44887
                     /gene="infA"
                     /locus_tag="U4A29_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO0679563.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            44920..45036
                     /gene="rpmJ"
                     /locus_tag="U4A29_000414"
     CDS             44920..45036
                     /gene="rpmJ"
                     /locus_tag="U4A29_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO0679564.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            45054..45419
                     /gene="rpsM"
                     /locus_tag="U4A29_000415"
     CDS             45054..45419
                     /gene="rpsM"
                     /locus_tag="U4A29_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO0679565.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            45447..45836
                     /gene="rpsK"
                     /locus_tag="U4A29_000416"
     CDS             45447..45836
                     /gene="rpsK"
                     /locus_tag="U4A29_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO0679566.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            45916..46854
                     /locus_tag="U4A29_000417"
     CDS             45916..46854
                     /locus_tag="U4A29_000417"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO0679567.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            46882..47262
                     /gene="rplQ"
                     /locus_tag="U4A29_000418"
     CDS             46882..47262
                     /gene="rplQ"
                     /locus_tag="U4A29_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO0679568.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            47534..48373
                     /locus_tag="U4A29_000419"
     CDS             47534..48373
                     /locus_tag="U4A29_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO0679569.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            48349..49218
                     /locus_tag="U4A29_000420"
     CDS             48349..49218
                     /locus_tag="U4A29_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO0679570.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            49208..50005
                     /locus_tag="U4A29_000421"
     CDS             49208..50005
                     /locus_tag="U4A29_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO0679571.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            50208..50954
                     /gene="truA"
                     /locus_tag="U4A29_000422"
     CDS             50208..50954
                     /gene="truA"
                     /locus_tag="U4A29_000422"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO0679572.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(51287..>53916)
                     /locus_tag="U4A29_000423"
     CDS             complement(51287..>53916)
                     /locus_tag="U4A29_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO0679573.1"
                     /translation="YAAASFLIPFLILALVYLSIGIYPGSSRSILASDAFSQFSNFHA
                     SFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFPNQMMPDALYVLTLL
                     KVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIMWLDAFVYLPLVILG
                     IHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLTNWSEYKKTIVPYG
                     ITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDLIMKNMIGVYDTTKY
                     GSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASFYLVPLNLFWHGMHA
                     PNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAALFAVAWGIKPEGSY
                     TYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSAEALVNTNSMIHGI
                     LDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSANDSINYGYSGISLF
                     SSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNISKNESTFSKYGFR
                     QAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLINTLSGLNEQYFTF
                     YTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQAYLSLFPTNFAQLE
                     SSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGTKEISFMEPKVVGLD
                     VVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDKGWKAKIDGKPVKV
                     SNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFILYVWYLDRNRPFYGT
                     IDPAIEEQPRNTHRRRRRK"
BASE COUNT        17194 a         9783 c        11654 g        15285 t
ORIGIN      
        1 gtgttctatc gaacacacag tacgttcaat aggtaaacta gctataacaa tagatattaa
       61 agttaacgga caagagggcc ttttttaggt agctaaaacg taataatgaa aatttatttc
      121 cagagatagg agtcaatcag ctagtctgat cgactgtttt aacaagttga ctttcatttt
      181 aacttttgat accatttaaa acacaaatcg taattgtttc gatttgtgtt ttttagcatg
      241 actacaatca aagaagaaag tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat
      301 accgccgctt gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag
      361 atgtgaaacg gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg
      421 aaaaacgttt ttcggctatt ttcaataatc ggtttttgtg taaggtttcc gaaagaactg
      481 ctgttgattt tccattttta acatatgata aattggtaga atctttcttt tcaaattgat
      541 accattccct aattccagtt atatagttaa ctacagaacc acaaagcacg tacgtttata
      601 aaagtattat ctgtagttat ttttcaattc cctttcaacc tcttattttg accagtagta
      661 ttgatcactt taccttttaa cacctttgtt cgtttttgaa acatttttag tcaagaatgg
      721 aaacgtaact acataatcaa aggttttgcg aaatatttca tgaaatattt catgtagaag
      781 cgagcaatac ggttgcaaat aaccattatc gtttaataag cagttacgaa agctcccaat
      841 atctctaacg ttcagctcac tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc
      901 tttctatttg tcgaaaaagt taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat
      961 aaaaaagcag ttactattgc tagaataaca tcaaggaata ataagttttg gttttcagat
     1021 aatagtcgat aagttgtgta ttggaatttt agatacagta ataaagtatt tttcatgaaa
     1081 ttttgtgaga atcactatta caagtatctt taataaaatt aatggttaag gaggaactta
     1141 aagtagaatg taaatgaata gttgtagtca gtcgattgtg atactagact caaagttgca
     1201 ggaataagtt tttcaatttg tgattggaag ataaaaaatg aaaaagaagg gctttttttt
     1261 atactttgtt gattgtagtg tttattttcc ctctgtttag ccaagtaaca ttttctatga
     1321 ctcaaccagt ttcaagtgag gaacatgata acctttttcg tgctacagaa gagtttaaga
     1381 aatatcagga aaatattgtt ccggagactc cattagctag tgagccaatt actgatattg
     1441 atggaaacca aataggatgg tatatacagt acgaagttat ggtggtaaat aatgactcaa
     1501 tagtaaaaaa tacagtaggc ttagagctaa caccgattga aaaggtgagt tcattgttaa
     1561 cgtatattta tactgatgaa agtgacgaga ctgagatcat tttaacggat tactcaaaaa
     1621 ttgctactga taaaaagtat atattgtgga tgtagacact agtgaaatac tttatgatat
     1681 agaaattgat gaaaatttac tagatgtagc acaagaaatc gatcatcaaa agcaggaagc
     1741 tattcgatat gctggtagaa aagggaaaag aaaatattta ttacgtgaat gtagcaaaag
     1801 ttagagaaga tgaaaatgag tggaaagaat tcaagagtcg ctactcaatt aactcgactc
     1861 cgacatttac agtttacaga gaaggatcga tagaaaaaac tgttttttgg actaaagaaa
     1921 gtggaatgtc tttagctgaa gtagaagagt ttttggatta tgttagtatg caacagtaag
     1981 ggagttctta ttatgaaaaa ttttgacaca tattatgtta ttgttttttc gatatctttg
     2041 ctgttaatat tactaacttt aatgcagtat aaagagtatc cagagtattt tattttaact
     2101 tttgtatatt tagtaagtag ctacacactt ctatataaat tcagaagttc ttctagagtt
     2161 tctaaagttt gtttcttaat tagcttattg attctataat atttttgatt tatataggaa
     2221 attaaaaata ttatagaatc aacactcaga gaacgggttg gtttttaata gatttaataa
     2281 gtactgataa tgaaatttct cctggttggg aaaacgaagt tggttaccct gtagaagaat
     2341 tttttcaaga ttactttgcg gagtatgccg ataggataat gagagattac gtaacagata
     2401 aaactcaaga agtcatcaat caatcccagt cattaaatga attatctgat aaggcgaaag
     2461 aagaagcagt tgccattatt tcttcaggga ttagtgacta tattgatatg agtggtgaaa
     2521 tcaattatgg cgataaagac aaaattgtac gcatgataaa agcttttatc aaaacttttg
     2581 atgtgaaggc agatcttaag tttaatgata cttacctagt aggaaaatta attaatatcc
     2641 ttgggacaga gaaagatacg gaaacgttta tttcctcttt gtctatgttg tttacagata
     2701 agcatttttc aactcagctt ggtggagagg aactagtgga aatcttttaa ccattacgcc
     2761 cagcattaat aaaagtatat caaaaaagta ctcctgatga tttttaagat tggtttgaga
     2821 aataatgtga aaacactatt tttaatgtta attatagata gagatgaacg cgtcatatgg
     2881 ttaaaagaat gaaaaagtca aagatacact gctatatttc actaaggata tagcagtgta
     2941 tctttcatgt cgtttcactg gcagtttttt ataaaaggag gtgtgcattt cagttgcttt
     3001 tctttggtag ttagttgaat cgtttaagaa ttaacggatg tggcagacag gtgctctgtc
     3061 caaaaaccag taaaatcttt gatggttcca tctttattta gctgtgcctg ttcaaatact
     3121 gttttgtttt taccgaatgg atcaattgca ataattgttc cgatccccgc acgtttggca
     3181 gcaagtaatc ctgagtaagc atcttcgata acaagacaat cagccggagc taagctcagt
     3241 ttgtctgccg ctttaagaaa aatatccggt tgcggtttcc cagggaaaga gccgtcgtca
     3301 tagacaatct tgtcccagtc aaaccagcgg gctaaatcga aaatatcaaa ataaaaagca
     3361 acattctctt tgacagtagc cgtggcaatc gttaatggaa tctgttggtc agtcagctgg
     3421 tcaagagtcg cttcaagccc ttttgtcaac tgaagctcgt tttcattttg caagcaaagc
     3481 atacgataat agctttcttt ttcttctgac aatacggcga tctcagaggc ggtcaaatgc
     3541 tctgaaatga aatgtttcaa aatctccgta ttcgtccgac catgaatatt ttttaaaata
     3601 tcctcgtctg ataaaccatc ttttgtatga tcatgtatca tatgcagcca agcagcttca
     3661 tgcagatgag agtccaaaaa cattgttccg ttgaaatcaa atataatccc tttcatagtt
     3721 acgctcctat agtggtattt tcggttgaat cagcaatttg cagatcgaca tattgtgcat
     3781 agtgctcttg aacctcccgc gagatctggc gatccgtaat gagcagatcg aaatcatgca
     3841 aattataaaa cacataaaaa tcatctcgat tgaatttttt attatcaacg agtagatatt
     3901 tcttacggga attatttaga gcgatctgct gtgcttctcc ttcttcggta ctgtaagtag
     3961 agatcgcttc gttgtgaacg ccatttgaac tgataaatgc ttttgtaaac tttagtcttc
     4021 ttaaattgtc gttggtgatc ggtccgacaa atgcgccggt atttttccgg aaatcaccgc
     4081 caatcagtag aatttctggt ccatctgctt gagcaagaat atcaaaaact ggataattat
     4141 ttgtaatgat ccgaattttc ttgttcagta aatgcgtcgc caatagttcg attgttgtac
     4201 ctggtcctaa gaaaatcgtt tctccctcgt gaatcaagga ggctgctgtg gcagcaattt
     4261 cttttttttc ttgaatctgc agagtggctt tttcagtgtg agaaagttct tgatctaaag
     4321 tgtaggaaag actttgagcc cctccatgga cacgcagcag cttgcccgat ttttctaatt
     4381 catctaaatc tctgcgaacg gtcatatcag aaacatctaa ttgattgatg atttcattaa
     4441 cggtaaggat tcctttttta ttgaccattt ctacgatagt aagcaatctt tcttttttca
     4501 acattgtctt cacctctaat ttaaggataa tctatcatat aaacggacaa cagtcaatct
     4561 aattcgaaca aaagtaaaca tttagggtgg tttttatgtt tgttacaata gattttattg
     4621 ctgttttctg gtttttatga ttatttgtgt ttgtttttgt tgacaaatgt tttggatgcg
     4681 tttacaatgc aatcagttag aaagagcgaa aactgattat agatttgagg agagatgtac
     4741 atgcttaaat taccagaaga ttttattttt ggcggagcca cagcagccta tcaggttgaa
     4801 ggagccacta aggaaggtgg caaaggcgct gttgcttggg atgacttttt agaggaacaa
     4861 gggcgattca gtcccgatcc ggcaagtgat ttttatcatc aatatgcaaa ggacattgaa
     4921 ttgtgtgaac gttttggtgt aaatggtcta agattatcca ttgcttggag ccgcattttt
     4981 cctgatggag ctgggaaacc aaatccagaa ggaatcgctt tttatcatcg tgtgttcgaa
     5041 gaatgtaaga aaagaaacgt aacgccgttt gtgacgttgc atcattttga tacccctaaa
     5101 cgtttgtttg accatggaga ttttttgaat cgagaaacaa tagaagcatt tgtttcctat
     5161 gccatttttt gttttcatga attcaaagaa gtcaaggtgt ggagtacctt taatgaaatc
     5221 tatccagttg caaccaatca atatttgtta ggtgttttcc caccaggaat caaatatgat
     5281 tttacaaaga tcgtcgcctg tcttcataat atgatggttg ctcatgcccg tgtagtgaac
     5341 tatttcaaag aaaacgaact tccgggtgaa attggtgtag ttcactcatt agagacgaaa
     5401 tatgcggcaa cagacgcgcc ggaagataag catgctgcct ttttagacga tgcattgtcg
     5461 atccgtttct tattagatgc tacctattta ggctattatt caactgaaac cttgactgct
     5521 cttgatgaga tatgtgaagc aaatcaggcc agctatcatt ttcctgaaga agatttcgtt
     5581 gaattgaaaa aagcatccac gcgtaatgac tacttaggga tcaatcacta ccaatgccat
     5641 tttgtcaaag cttacgatgg agaaaatgca atccatcata acggtacggg tgaaaaaggg
     5701 acctctgttt ataaagtgaa gggaatcgga gaacgtattt ataaagaagg aattcctcga
     5761 actgattggg actggctgat ttatccagaa ggtctatatg acttgttgct gcgaattaaa
     5821 agcgattacc cacattacaa caaaatctat atcactgaga acgggatggg gtataaagat
     5881 cagtttgaag atggcattat tatggatcag ccgagaatcg actatttgag agtctatttg
     5941 gaatccttaa gcaaggcgat aacagccggg gtaaatgtga aggggtattt tttgtggtcg
     6001 cttatggatc ttttctcatg gacaaatggg tataacaaaa ggtatggctt gttttatgtt
     6061 gattttgaaa cccagaagcg ttacccaaaa gaatcagctt actggtacaa gttggtcagt
     6121 gaaaccaaaa cgattattta ataagtgata atggaggaag aaaagatgaa taaagaggaa
     6181 ttacaaatgc ttggttttga gatcgttgca tattcaggcg acgctcgcag tacattattg
     6241 aaattattga aagaagtaag acaaggaaac tttgaacatg ttgattcggc gttgaaagaa
     6301 gccgacgaaa atttaaccct tgcacataat tcgcaaacaa aaattttagc ggaagaagca
     6361 tctgggaaag agatggagat gggattcatt tttatccatg ggcaggatca tttaatgacc
     6421 actttattgc tgagagatct gattcaagac tttattgtac tgtacagaca gaatcaagga
     6481 taggagaaga acgctatgga tggaatcatt aaacaaatcg aaaaagggaa accatttttt
     6541 gaaaaaatct cacgtaatag ttatttagga gccgttcgtg acggattttt aacagcgatg
     6601 ccagctattt tattttcaag cattttcatt ttgattgctg cggttcctga gatttttggt
     6661 taccaatggc ctgaatctgt ctccacatgg ctatggaaag tctataatta ttcgatgggt
     6721 gttgtagggt tactcgtttc agcaaccact gctcgctgct tggctgattc gatgaatcgt
     6781 cgcttaccga aaaacaaagt aatcaacacc acctctgtga tgctggcctc tgtggtaagt
     6841 ttcctgttgt taagcgtctc tccgattgaa ggtggatttt caacggaata tatggggaca
     6901 aagggactac tggcttcctt tgtcgctgca tttttaacag ttaatttata taagttttgt
     6961 gtgctgaaag atattaccat taaaatgcct aaagaagttc ctggaacgat ttcgcaaacc
     7021 ttccgagata tttttccttt ctctttttca gtatttgcgg ccgttttaat tgatttaggc
     7081 attcgacaaa tcttccaaat ctcatttgca gaagcaatca ttacggtgct gcagccgcta
     7141 tttacagcag ctgacgggta cttaggaatc gctattatct ggggagcgat ggcgctgttt
     7201 tggtttgtcg gtgttcatgg accgtcgatc gtggagccgg caattgcggc aattatctat
     7261 gccaatgtgg aaaccaatct gcaattgttc aattccggtg aacaagcttc taaagtactg
     7321 acggtgggtt tagggaattt tgtaggaact atgggaggaa ctggggcaac attggttgtt
     7381 ccttttatct tcctactctt tgctaagtct aagcagttga aggcagttgg gaaagcttcc
     7441 tttattccag taagtttcgc tgtcaacgaa ccattgctat ttgcggcacc catgattttg
     7501 aacccttatt tttttattcc atttttgtta acacctgttg taaacgtttg cctgttcaag
     7561 ttttttgtcg atgtattggg catgaacagc tttatgtacg ttttgccttg ggcaactcca
     7621 gcaccgatcg gtttggttct aggaacgggc atgggtatct tagcttttgt tctagcagcg
     7681 gttctaatcg tcgttgactt cctgatctat ctaccatttt gcaaagcata cgatgctgtc
     7741 ttagtagaga aagaagcaca aaaagcagag gtgatcgcaa ccgaggaagg tgcttctgcc
     7801 gatgtacgtg aggaagtacc tgtggcagcg agtcaagtta tcagcgatgc aaataaagaa
     7861 atcaatgtgt tggtactttg tgctggcgca gggacaagtg cgatgttagc taatgcttta
     7921 aaaaaaggtg catctgaatc gaaagcaccg atcagtgcaa tggcgggtgc ttatggttct
     7981 cattacgaaa tgctgcagga ttttgatatg gtggtcttag caccgcaagt caattcttac
     8041 tatgatgata tcaaagaaga caccgatcgc ttaggaatca agctggttgc gacaaaagga
     8101 gcagaatata tcggattaac gagagatcct gaaaaagctg ttcagtttat tctgaaccaa
     8161 tttcaataaa ggaggagcca taatgaaaat gggaatttgg ttgcgtaaag ctgttttatt
     8221 tgcagctgtg ttaggggctt tgggggctgt ttcaaaaact gttgaagcat ctgccagcga
     8281 atttattcgc ggagcagata tttcgatcct gtcagatatg gaaaaatctg gtgcaaaata
     8341 ttatgaagac gatatttcaa aagatgcctt agagatttta aaaaataatg gtgtcaacta
     8401 tgttcgactg cgtttgtggc aggatcctta taatagtaat ggagaaagtt atggagcagg
     8461 gacaaatgat ctggaaacga cgattgacct tgcaaaacga gcaaaaaatc tgggacttaa
     8521 ggtgctgctg gattttcact acagtgattt ttgggttgat cctgggaaac agaatcttcc
     8581 taaagcatgg caaggtctga cctttgaaga aatgaatacg gcattgtacg actatacaaa
     8641 gaacgttctg tcggaaatga agcagctgga cgtttatcct gatatggttc agatcggcaa
     8701 tgaactgaat agcggaatgc tctggcctta tggcaagtct tggggagaag gcgggggaga
     8761 atttgatcga ttggctgctt ttttgaagtc tggtatccaa gctgttcgag acactcaacc
     8821 taaaacaact ccagtaatgc tccacttagc tgatggtggc gatacaggag catttacttg
     8881 gtggtttgat gaaatcacga gtcgaggtgt gtcatttgat ttaattggag tgtcgtatta
     8941 tccgtattgg cacggttcaa tgaatgaact tcaaaccaat atgaatgcga tcagccaacg
     9001 gtatggtaaa gaagtgatcg tagttgaaac agcttatggg catacaacag cgaatgccga
     9061 tactatgcca aatgcttttg gtgaagccga agcagctgcg gggggatatg aaccaacacc
     9121 gacaggacaa gcagagtatt tgctggatct tgccgatcgg attcaggcag taccgaataa
     9181 tcgcggggcg ggcttcttct attgggaacc tctttggtac aacggaaatg tgagttgggc
     9241 aacacaagca ggtatgaatt atctcagtgt tcaatccact atgggaaacg aatgggacaa
     9301 ccaagcgatg tttgattttg ctggaaacgc cttgccaagc ttgcgtgcgt ttaaacaagc
     9361 aggggcgcaa ataaatctag ttaaaaatgc aagtttcgaa agcgatggac caacgaccag
     9421 tccttccaat tggcaaacct ggtttcaaca aggaaccgct aagggtacga tcaaaacgga
     9481 agcgggcact ttttttgggg actacaaact aactttttgg caggatcaag catacgaggc
     9541 atcagcttat caaaatgtta gtggcataag caaaggaact tacacattga gtgcctatgt
     9601 aatgtctggg ggagagtttg atactaatca gttatatatc aaaaattatg gtggagcgga
     9661 actaaatact gcggttgtta aaacaagtga atggacaaaa attacaatca aagacattcc
     9721 aattacaaca ggtgtttgtg aaattggggt ttatagcaag gcttcaggaa atgcttggac
     9781 gaacgttgat tttgtttcat tggtgaaaca atagactgcg gatcgtttcg attcaagatc
     9841 ggaggttccc ttcagccaca tcgttagcgt agaaggcagg aatcgaacct gcacctcgcc
     9901 tttgccaaaa ataaccagaa acgatttcca gtcattttta ccaaaacgag taaagcacca
     9961 gacttcatct acactttgta ttatgctaaa gagagttcaa cgatttcgta agaagcaatg
    10021 aaaaaattgc aaaaagttgt gcagataaca taaagtttgt ttaagaaagg gtaaccttag
    10081 aattccgtcg tgaatatgat ttgagcactt agatggatag aaaaaagttc tcggaaaaca
    10141 gttcgttttt cgagaacttc tttttatgat aagaatgaaa caaattaact tatattatag
    10201 tctcttttgg tagggaacca tcttattttt aatgaaaatc aagcatttta gctattgaag
    10261 aatgttgatt tatagggatt ccccctccat attatgttgg ctctttattc ccactccact
    10321 gttgctggtg gtttagaagt gatgtcatac acgatacggt tgacgtgggc tacttcgttt
    10381 acgatacgta cagagatttt ttgcaagact tcccatggga tacgggcaaa gtcagcggtc
    10441 attccgtcga ttgaagttac agcacggata ccgactgtgt agtcgtaagt acgtccgtca
    10501 cccataacac cgacagagcg gatccctgga agaacagtga aatattgcca gatatcacgg
    10561 tcaagtccag cagctgcgat ttcttcacgc aagatagcat cagattcgcg tacgatttct
    10621 agcttgtctt cagtgatttc gccaagaaca cgaataccaa gaccaggacc agggaatggc
    10681 tgacgccaaa cgattgaatc aggcatgcct aactgagtcc ctaattcacg gacttcgtct
    10741 ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg cagtccacct
    10801 acattatggt gagatttgat cgtctgtgct gtttctgtac cagattcgat aacgtcagta
    10861 tacaacgttc cttgtgctag gaaagagacg ccttcttcac cagcaagttt tgttgcttcg
    10921 tcatcaaaaa catatacgaa ttcattccca atgattttac gtttttgttc tgggtcagat
    10981 acacctttca acttgtctaa gaagcgatct cttgcattga ctttgatgat gttcaagccg
    11041 aattttccgc ctagactttc cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg
    11101 tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca
    11161 ctagaatcaa cgccacctga aagaccaagc aagacttttt tgtcaccaac ttgttcacgg
    11221 attttttcta cttgcatatc gataaagtta tccattgtcc agttaccttc acagtgacaa
    11281 acatcaaagg caaagtggcg cagcaattcg attccgtatt cggaatgacg tacttctgca
    11341 tggaattgga taccataaaa attacgtgtt gtgtcttgga tagaagcaat cggacaatct
    11401 ttgcttgttc cgactgtttc aaagccttca ggaactttag ttaccaagtc accatggctc
    11461 atccatactg tttgttgttt tggtgtttct ttaaacaaga cagcttgatc gcttgttact
    11521 tctaacaagg ctttcccata ttcgcggttt ttagcaggct ctacggaacc gccaagtttg
    11581 tacgtgatca attgcatacc atagcaaatc cctaatacag gaatacctaa attgaagatc
    11641 tcaggatcga tcccaaatgc attctcatca tatacactgt ttggtccgcc agaaagaatg
    11701 attccctttg gattcatcgc ttttacttct tctgcagtaa tgcggtggct caataattcg
    11761 gaaaagactc caagatcacg aatacgtctt gtgatcaatt ggttatactg actaccgtag
    11821 tctaatacga taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa atttattacc
    11881 cctatccctt taaattttat ctcaaaatag tcaaactatt ttaaaatgct aatattttcg
    11941 catgaaaatc gaatcgatga tatgattttc agttttatgg agaattatat cggctcttcc
    12001 tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc acatttttcc atacatcttt
    12061 tgccatttta aaggcttctt cacgatctcc gatagcaaaa cgataataat aattctctgg
    12121 attttggaaa gccgtatcca gtaacgcacc aaaacgatcc aaataccatt tttcgatcag
    12181 cacgggatcg gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt agatttgctg
    12241 attcgcaggt aattgaagcg tattgatccc ctcaacgatc aagatatcag gctgctggat
    12301 caattcgtac ttgtctggaa tgatatcata cacatcatgt gagtaaacag gtacttttat
    12361 ctcttcctga ccagatttta cacggttcaa gaaatcgatc agcatctcca tatcataact
    12421 ttcaggaaat cctttacggt ccatgattcc acgctctttt aatatacgat ttggataaag
    12481 aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt
    12541 ctgcagcagt ctggctgttg tactttttcc aacggctaca cttccggcga tcccgataat
    12601 aaaaggcggt acttctacgt atttatgaag aaataaacct ttactcaaga acaaagattc
    12661 aaattctttc atataaagat aaatcagatg agcaagaggt acatagatat ctcgaacatc
    12721 ctgcatggaa atgcggtcat tcaaactttt gatattatct agttcatctt gtgtcaaagg
    12781 tgcttgccca ttacgataaa attctcgcca ttcttctctt gccacacggt agtagttcat
    12841 tggatcattc atattagcct cctgcgttct cccatcaatg gaactatttt aacacaaaat
    12901 aaacgcaaac cccaccactt aagagagatt gccgatcttt ttgtataatc tttataatag
    12961 aaagaaacgg agtcttattc ttgacttgcg cacggataca taaaaggata gactgttgtc
    13021 atgaaagaaa tgagaaggat ggaagacatg acaaatcatt actattctga acgcccagat
    13081 acggcgcacg attttgaaca atgggctttt gagttaaaag gcagaacctt tcaatttgta
    13141 acagattctg gtgtattttc acgtgaaaca gtggattatg gttcacgtgt cctgattgat
    13201 gctttcgatt gggaagagtt acctgatgaa ggaaagatct tagatgtggg atgcgggtat
    13261 ggaccgattg ggttagcttt ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt
    13321 aatgaacgag cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt
    13381 gatatccatc aatcgaatat ctatgaagca gttcatgaag aaacctatgc agctatcgta
    13441 agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac aggcgcatat
    13501 cctcggttga agaagggcgg tacactgaca attgtgatcc agaagaaaca aggagcgccg
    13561 agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag aaatcgtcac aaaagataaa
    13621 ggatattata tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa
    13681 agagtggaac tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt tgagaagttt
    13741 ttaaaaacaa agaactattt tttcttcaaa aagaaatagt aggaacagag tcgttgtttt
    13801 ttacgataaa ataaataaga tacagaactt gtatagaagg ctgccaagtc ggtagataaa
    13861 ggaggttttt atcaaaaaac atactttcct tttttctcta ttcttttctt gggggttttg
    13921 gtacactgat agggaattta gatagaaggg agccaatagg atgaagctga ccatcagaga
    13981 tatcgcagaa atggcaggcg tatcagtaac aactgtctca cagattttga ataacaaagg
    14041 aagcagattt agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta
    14101 taagcccgat tatttcgctt caaatgtgat caatcgacat tcaaagacca tcggaatgat
    14161 cgtccctgat gtcactgatt tcttcttctc caaactgatc gaaggtgtag agagctattt
    14221 gaatcctttg ggctatgtga tcatgttatg caattctcgt catagccaag aaaatgagat
    14281 caagtattta caagagcttt cccaccgctc agtggacgga atcttgctgg ccacaccaaa
    14341 tattttacct gagaaatatg cattagacag cggtttttat caaaaaatgc caatcatttt
    14401 aatcgatcgc ggattgaatc gtcgggataa cggacggctt atcgtaaagg aatatgaggg
    14461 cgcctatcaa gctgtttcgc tgtttattga aaatggccat acgaagatcg gcatgctgaa
    14521 ggaaaccact ggatactatc aattagaaga acgtttcaac ggatatcgtc atgcgctgaa
    14581 agatcatggc ctgcctttta atgggaaatt tgttgaacat ggagagttga ctgtccaagg
    14641 tggatatact gcatcaaaaa aattactcaa acataaagat atcacggcta ttttttgcgg
    14701 gaatgatgcc atggcgatcg gatgctatca ggcgatcgat gaattgggca aaaaaatccc
    14761 agaagatatc tcagtcatag gattcgatgg attaaagctg tcagaataca tgattccgca
    14821 attgacgaca gtgcagcagc caagttttga tattggattt tatgctgcac gtttcctgat
    14881 cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat
    14941 tattcgtgag agcgtaaaag tattgacaaa gggctaactc ggcggtatat tgcttatgta
    15001 agcatttaca aaggctagtt agtgcttttt ttatttttag taaaccagtt tactaaagtg
    15061 gtttaccttt cctaaaaata atgtataata ccgaatgaag acaaggagga tcaggcaatg
    15121 cgaatggtgg atctgattga aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc
    15181 gagtacatcg tcacgaacta tacgaatgga aagattccag attatcaagt cagtgcatta
    15241 ttgatggcaa ttttttatca agacatgaca aatgaagaaa tcacgaactt aacattggcg
    15301 attgctaatt ctggtgatgt catcgatttg agttctcttg aaggaataaa ggtagataag
    15361 cattcgacag gtggcgttgg tgacacgacc acattgattt tagcccccct agtagctagt
    15421 gtcggagtga cagtggctaa aatgtctgga cgcggtctag gttacacagg tggtacacta
    15481 gacaagctag aagcaattcc cggatttcag atcgagcttt cggatgaaga atttgtacgc
    15541 attgttaatg aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc accggcggac
    15601 aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg attctcttcc gctgattgcc
    15661 agttcgatta tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt ggatgtgacc
    15721 actggagacg gtgctttcat gaagaacatc gaagatgcaa gacgtctggc aaagaccatg
    15781 acgagcattg ggaaactggc taaccgagaa acagtggcgg ttatctcaga tatgtcagaa
    15841 cctcttggcg aagctatcgg aaatagtcta gaagttgtcg aggcaatcga aacattacaa
    15901 ggaaatggtc cagaagatct agtagaaatg tgctacgcct taggaagcca aatggttgtt
    15961 cttgctggca aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa
    16021 tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa atcaaggggg agacccagcg
    16081 atcgtggaac atccagaacg aattttgact gcacgctaca caatggagct tcctgctaaa
    16141 caaagcggag ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc aatgatgcta
    16201 ggtgctgggc gtaaaaccaa agaagaagat attgatcacg ctgttggatt gaaactgcat
    16261 aaaaaaattg gtgacactgt gacaaaaggt gagtcactat tgacgatcta tagtaacgac
    16321 gaagaaatca cttctgtcat tgaattatta tataaaaata ttgaaattgg tgaatcggca
    16381 atgaagccaa cgttgattca tgacatcatc acagaataaa ggaaaaaagc ggactttacc
    16441 gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca tactattttg
    16501 aaagcagacg catctaaaga agctgtgatg caaattatcg aagaagcaaa aaaataccat
    16561 ttttattctg tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca attacaaggt
    16621 gagccagtag ccgtatgtac agtaatcggg tttcctctag gagcaaacac acccgagaca
    16681 aaagcatttg aaacaacaga tgccatcaat aacggtgcag atgaagtcga tatggtcatc
    16741 aatatcggtg ctttgaaatc aaaggatgat caacaagtcc aaaaagatat cgaagcagtt
    16801 gttgaagcag caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc gttattagat
    16861 agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa
    16921 acatcaactg gtttctcaac aggcggagca aaggtagagg atgtccgttt gatgcgtgaa
    16981 actgtaggac cagaaatggg tgtcaaagct tccggcggta tccataacga agaagaagca
    17041 atggcaatga tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt agcaatcgta
    17101 tctggttcaa ctggtgaagg ctattaattt tttcaaagga gaagctcatg gcagaactca
    17161 aacaagaatg gatcaacaca gctattgaag cgttagataa agcctatgtt ccttattccc
    17221 attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca
    17281 ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag
    17341 tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc cccgatccta
    17401 tctctccttg tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct gatatgccgg
    17461 ttacgctagt aggagacaat ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt
    17521 atgcatttac tgaaaaagat ctataactca tgttcattca gtcagctgac tgttgaaaaa
    17581 taaaacaatt attttaggag gctttacaga aatgaaaaaa gcaaaattat ttggtttagg
    17641 tgctgtcgca ctagcagcag ggttattttt aggtgcatgc ggcaacaacg gctctactga
    17701 ttcatccggt ggcaaaagct catcagacac aacaactgca gcattgatca cagacacagg
    17761 tggtgtagac gaccgttcat tcaaccagtc agcttgggaa ggactagaaa aatgggggaa
    17821 agatcaagga ctttcacgag gaaatgatgg cttccaatat ttccaatctt caaatgaatc
    17881 agattacatc ccaaatattg accaagcatt gaatgctgga ttcaaaacga tttttggtat
    17941 tggttataaa ctaaaaccag cgatccaaga acaagcaaca aacaatacag gtacgaactt
    18001 tgttattatc gatgatgtca tcgatggatt agataacgtt gtatctgcaa ctttcaaaga
    18061 caacgaagct tcttatcttg caggtgttgc agcagcttat acgactgaaa caaacgtagt
    18121 cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc
    18181 cggtgttgat gcaggtgcga aagaattagg aaaagaaatc aaagtattga atcaatatgc
    18241 tggtgacttc tctgctccag ataaaggacg ttcaattgct caaggtatgt atgcacaaaa
    18301 cgcagatatc atcttccatg cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa
    18361 atcattgaac gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc
    18421 agacgaaggc gaatacacat taaacggcga aaagaaaaac ttcacattga catctacact
    18481 gaaagcagtt ggaacagtag tagaagattt ggcacaaaaa tcagcggacg gtaaattccc
    18541 tggcggcgaa cacactgttt atggattgaa agaagacggt gttggtctaa cagaaggaca
    18601 actttctgac gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga
    18661 cgtaaaagtt ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca
    18721 catgtttgac aaggctatgg tttgattaga acaagaattg ttcatttatc caagctgcct
    18781 tgtgacagtt tggataaggg aacaactccc tgaagtaatg tttgtttcaa aagaagaacg
    18841 ctcactaagc gtgaatctta gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa
    18901 aaaaggtggt tcaagaaaat attgtcatag aaatgcagaa tattaccaaa gaatttggaa
    18961 cattcaaggc aaatgacaac atcaatttac aagtaaaggc aggagaaatt catgcgttgc
    19021 ttggagaaaa cggtgctggc aaatctacat tgatgaacgt gctttccgga ttattagagc
    19081 cgacatcagg gaaaattttg atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag
    19141 ccaaccaatt agggattggg atggtccatc agcactttat gcttgttgat gcctttactg
    19201 taacagaaaa catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa
    19261 aagcacgaaa agagatccaa aaagtttctg aacagtatgg attgtcagtc aacccagatg
    19321 cttacgttcg tgatatttca gttggaatgg aacaacgggt agaaatttta aaaacacttt
    19381 accgaggagc agatgtactg atttttgatg agccgacagc tgtattgacc cctcaagaaa
    19441 ttgatgaatt aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg atcattttga
    19501 ttacgcataa gttagatgaa atcaaagcag tagctgaccg ctgtacagtt atccgccgtg
    19561 gaaaaggaat cggtacagtc aacgttaaag acgtaacctc acaacaatta gccgatatga
    19621 tggttggaag aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg
    19681 ttttgtctat tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact
    19741 tgaacttaga ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc
    19801 agtcggagtt gatccaagct ttgactggtt tgcgaaaggc agaaagcgga catatcaagc
    19861 taaaagggga agacatcact aataaaaaac cccgaaaaat cactgaacat ggtgtaggac
    19921 acgtgccaga agaccgtcat aaatacgggt tagtcctaga tatgacatta tctgaaaata
    19981 tcgcactaca aacctatcat caaaaacctt acagtaaaaa cggcatgctg aattattcag
    20041 taatgaatga acatgccaga gaattgatcg aagaatatga tgttcgaaca acgaatgaac
    20101 ttgttcccgc aaaagcttta tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag
    20161 tcgaccgaga tcctgatctg ttgattgttg caaatccaac tcgtggactg gatgtaggtg
    20221 cgatcgaatt tattcataaa cgtctgatcg aacaaaggga caaatacaaa gcagtgttac
    20281 tgatcagttt cgaattagaa gaaattttaa atgtttcgga tcgtattgct gttatccatg
    20341 aaggagaaat tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa ttaggtctat
    20401 tgatggcggg ctatacctta gaagaagcac gaaaagagtt agaaaagggg gctggcgcac
    20461 atgaatgatc gcagcgaaaa attccgtaat ttattagtgc ctgtactttc agttgtttta
    20521 ggattgatct taggcgcgat attgatggct gcttttggtt ttcagcccat ccaaggttat
    20581 tcttctatgc tagatgcagc ccttggttcg caaagaggga taggtgaaac gttaaggcaa
    20641 gcaacgccgt tgatttttac agcattaggt ttttctgtag ctaactcagc tgggtttttc
    20701 aatattgggc tttctggaca agcactttgc gggtggatcg tcagtgtctg ggtggcactt
    20761 gcgtttccag atctgccaaa agttgttttg cttccgttat gcgtgatctt aggtgcctta
    20821 gctggtgcga tggcagcggc tgttccaggt ttacttcgag cattttttgg aacaagtgaa
    20881 gtgatcgtaa caatcatgat gaactatatc ttattatacg taagcacatt tgctttacat
    20941 gaagtgatgc ctgaaagcta tcgctcaagt ttggactctt ccaatatgat cagtcaaaat
    21001 gcctcactgc ggttaccatg gttaacacaa gcttttggcg gctctcgcgt aaactcagga
    21061 ttatttttag caatcatcgc gcttattttc gtttggctgc tgatgaagaa aacgacttta
    21121 ggatttgaaa tccgttctgt tggattgaat ccttttgctt ctgagtatgc aggaatgagt
    21181 agtaaacgta cgatcgtttt gtcgatggtc atttctggcg ctttggcagg gcttggcggt
    21241 gtggttgaag gattaggaac gtatcaaaac ttctttgttc aaacgacttc gctatctatt
    21301 ggatttgacg gaatggctgt ttccttgctt ggttctggat cagcaatcgg tattctttta
    21361 tcagcgatct tgttcagtat tttgaagatc ggtggacttg gcatgcagac aggagcaggt
    21421 gttccgtttg agatcgtcaa tgtatcgatt gctttgatta tcttctttgt cgggatcaac
    21481 ttcctgattc gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt
    21541 gcgacaatag aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact ataatgtcga
    21601 ctatcgaatt aattgccttg atccttactt ctacactggt ttattcaaca ccgctaattc
    21661 taacgtcact aggcggaact ttttctgaac gaagcgggat cgtaaacgta gggctagagg
    21721 gaatcatggt catgggtgct tttagttcca tcgttttcaa cttgacgatg agtccatcac
    21781 ttggttctat gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt atcctatttt
    21841 ctctgattca cgcagtagca acagtcagtt tgcgtgctga ccatattatc agcgggacag
    21901 taattaatct gatggcacct gctttaggtg ttttcttgat caaagcatgg tatggtaaag
    21961 gtcaaactga taacatcacg cagaatctgg ggtatttttc tttcccagga ttagcagata
    22021 ttccggtcat tggaccaatt ttctttaaaa atacattctt acctgcttat tgtgctattt
    22081 tgattgctgt cctagcttgg gtgatcatct tcaagacgaa attcggcttg cgcctccgtt
    22141 cagttggtga aaatcctcaa gctgccgaca cattaggtat caatgtatat gctatgcgtt
    22201 attcaggcgt actgatttct ggcttgttag gcggtatggg cggtgctgtt tttgctcagt
    22261 cgatttccgg taacttttca gctggtacga tcgttggaca agggttcatc tccatggctg
    22321 ctatgatctt tggtaagtgg aatcctttag gggcaatggg tgcctcattg ttctttggtt
    22381 ttgctcaaag cttgagtatc attggcgcac aattaccagt tatttcttcg attccgccag
    22441 tattgctgca aattgctcca tatttgctga caatcattgt gctggttgtt ttcttaggga
    22501 aagcttctgg tccaaaagca aacgggaaga actatatcaa atcaaaataa tcttttagcg
    22561 aataaaaagg gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa
    22621 taaaaataaa aagaaagaag gaataatcat gtttaaacgc gtacatctga tcgtcatgga
    22681 ttcagtagga attggagaag caccagatgc cgataagttc ggtgatgttg gttctgatac
    22741 attaggacac attgcgaaag aagcaggatt gaacgttcca catttagagc agcttggttt
    22801 aggaacgatc cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac
    22861 gaaattagaa gaagtttctg tagggaaaga tacgatgaca ggccactggg aaatcatggg
    22921 attgaatatc aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa
    22981 acaaatcgaa gaattttctg gccgtaaagt cgtatgtaac aaaccttata gcggaacagc
    23041 tgttattgac gattacggtg aacaccaaat gaaaacaggc gatttaattg tatatacttc
    23101 tgccgatcct gtccttcaga tcgcggcaca tgaagaaatc attcctttag aagaattata
    23161 tcggatttgc caattcgttc gtgatataac aaaagatgat ccgtatatga ttggccgaat
    23221 cattgcacgt ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca atcgccatga
    23281 ttatgcgttg aatccattcg gacacacagt ccttgattct ttgaaagaag caggaaagga
    23341 cgtgattgct gttggaaaga tcaacgatat tttcaacgga caaggaatca ctgaatctgt
    23401 ccgcacgaaa agcaatatgg atggcgttga ccagctgctg aaagttatga aaaacgattt
    23461 ttcaggaatc agctttacca atttagtaga ttttgatgca ttatacggtc atcgtcggga
    23521 tgtagtcggc tatgcgcatg cgattgaaga atttgactta cgaattccag aattgcttga
    23581 agcgatggaa gaagacgact tgctaatgat tacagccgat cacggaaacg atccgacgtt
    23641 tactggtacc gatcatacgc gagaatatgt accgttattg gtttacagta aaaaaatgaa
    23701 agaacaaggg gcattgcctc aaggatatta ttcagatatt tcagcaacga ttgctgagaa
    23761 ctttagtgtt tcttcgactg aaaatggcga aagcttcttg aaattattaa aataatcttt
    23821 ttaaatagga gaaagcaaat gaccaagtta agcgagatgt tacaagaaac gacccagttt
    23881 atccttgata aaggagtaaa agaaattgat tttggtttga ttctaggttc aggattaggt
    23941 gaattagcag atgaaatcga agatgctgta atgatttctt atgatgagat cccgttcttt
    24001 cctgtttcga cagtagttgg gcatgcagga aagctagttt atgggtctct ggcaggcaag
    24061 aaagtgttag ccatgcaagg gagattccac tactatgaag gtcattccat gcaaacagtg
    24121 acctatccag tacgtgtgat ggccggacta ggggcccatt cagtcattgt gactaatgca
    24181 tgtggtggtg tcaacgaatc atttgtacca ggagatttga tgctgattac cgatcatatc
    24241 aattttactg gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg ccctagattt
    24301 ccagatatga gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa
    24361 aaactaggtt tggatcttaa agaaggcgta tatatgggct attctggacc aacttatgaa
    24421 acgcctgcgg agatccgaat gtctcggaca ataggagccg atgcagtagg gatgtcgact
    24481 gttccagaag tcatcgtagc agctcacagc ggattaaaag tgttaggaat ctcttgtatt
    24541 accaacttag cagcaggtat gcaagcgaat ctgagccatg aagaagttgt agaaacaact
    24601 cagagagtca aacaatcttt taaagcattg gttaaagaaa cattgacatt attgtaatag
    24661 gaggaacaat aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg ccgataagat
    24721 tttacttccg ggagatcctt tgcgagctaa atatattgcg gagacttttt tggaagatcc
    24781 agtttgttac aatcaggtac gcggtatgtt aggctttacc ggcaaatata aaggagaacg
    24841 tatttctgtt caaggaacag gaatgggaat gccatcagcc accatctatg cccatgaatt
    24901 gatccagtcg tatggtgtaa agaaattgat tcgagtaggt acatgcggtg cattgtcaaa
    24961 agacgtccat gttcgtgatt tagtactagc acaaggtgca gcgaccagtt cttcgatgat
    25021 cgaaaaaaat ttccaagcct ttcattttcc tccaatcagt gatttcaatc tattactgaa
    25081 agcatatgag atagctaaag aaaagggcta tacggttcat gtaggaaatg ttctttcgga
    25141 agattctttc tacaaagatg atttgacaga gacgttccaa ttagctgaac ttggcgtttt
    25201 aggagtggaa atggaagcag cggctcttta ttacttaggc gcgaaatacc atgtgcagac
    25261 attaagtttg atgaccgtta gcgaccattt gatcaccggt gaagaaacaa cagctgcaga
    25321 acgtcaatct acgttcaatg aaatgatcga agtaggattg gaaacagcga tagcagaata
    25381 agaaggaaat gaaaaagctg ccctgcatta agtaataaga acatccaagc aaaaatagct
    25441 cctcatgttt ttgacttggc tggacttatt attgcaaatg taagcttttg aacaccgttt
    25501 accaagaggt tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca
    25561 gcttttcatg tttgatgaat attgtgtctt gtgaccgaga agttggcagg agaaaaccgt
    25621 ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc ccttttttat agtgtttttt
    25681 ataggtagta ttttttagca actgttaagt catcatttaa ttcataaaca agtggttgac
    25741 ctgttggaat ttcaagatcc atgatgtctt cgtcagagat accttcgata tgtttagcta
    25801 gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt tttgttgtct agtaatgctg
    25861 gagcaatttc atcttgccag aatggcaaag cgcgttctaa tgtcactttc aagttttctc
    25921 caccaggaat atcgcgtttg tctaacattg cgtaacgacg gtcatttgct gcagaacctt
    25981 cgtcagtttc ttccattaat ggaggaagag tatcgtaaga acgacgccag atgtgcactt
    26041 gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa accttgtaat ttaccatagt
    26101 gacgttcgtt taagcgccat gatttgattt gtggtaccca aagttgatct gaatattcta
    26161 agatcaagtt acatgttttg attgcacgag ttaaaacaga agtgtaagca acatcaaatt
    26221 cgattccagc ttctttgatt ttgcgtccgc cttcttttgc ttcttcgatt ccttctggtg
    26281 ctagatttac atcagcccaa ccagtaaatt ggttcaatgc attccattca ctaagaccgt
    26341 gacgagaaaa aactaatttt ggcatgttta gctctccttt taagataggt ttatatttct
    26401 gaaattttca gttctgtttt attgtacaat tttttggaag agatttccat cgaaaaatta
    26461 gataagattc tatgagaaaa caagtagaga atataaaaaa acgaacagtg cccacgcaac
    26521 tgttcgtttt taggtattag atgcataatt gctcgatttc tttttcaggt aaattgatat
    26581 atttataaca tgttccaact gaaacaccgc attttgaaga aataaattgg atcgtttctt
    26641 ttttttcata atagagacgt cggatttttt tgatcgtttt tgcatcgatt tttggtcttc
    26701 cgccgatttt tcctttttta cgagcttcat ctaatccaac taatgtacgt tctttgatca
    26761 atgcgcactc catgtttgct aggttttcca ttaattggaa atagattttt cccatttcgc
    26821 gcgtatcgat tccttcatca agactaatca agtgcagccc gcgatctttg aataattgcg
    26881 tgagttctgt taattgccgc gtagatttgc ctaatcgatg aagttggcaa atgactagtg
    26941 catcgccttg ttgcatgtca gcgatcactg actccaaatg aacagcagta gaatcatttt
    27001 gttccacttc atatgtctca tggaaaattt cttgacaacc aaagtcagat aattgatcaa
    27061 cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata attttcatgg
    27121 tgattccttt ctagcagtga ttttctaact ggatataaca gtaaaaatgt agcataaagt
    27181 acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct ttttttattg gatgatcaag
    27241 cagtttttct ttatttacga cggattgcgt caagaatctc aacgcctttt tctgagccga
    27301 caactacttt agaaaccatg tttaggaaaa gcccatgttc tactacccca acaagttcat
    27361 ctaaataggt agctaagctt tctggctgtt cgatgacttc tagatgcaga tcaatgatgt
    27421 aatgaccacc atctgtggtt aagacttctc cgttttcgtc tgtacgcaag acgggttgga
    27481 atcttttttc ttcaaaaata tgaagtagct gttgggaacc ataagggatc acttcaactg
    27541 ggagtgggaa cttcccaagt ttatcgacta atttagaaga atcaacgatc caaatcgttt
    27601 cttttgagta cgtagcgact attttctcga aaagcaaagc agcgccgccg cctttgatcc
    27661 cttgaaaatc agcactgatt tcatcagcac cgtctattgt caaatcgact acggggactt
    27721 catcaatact ctttaacggt attcctaatt tttccgcttg ttcttttgtc gcatttgaag
    27781 tagtaactcc tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc tcttctacca
    27841 tataataggc ggttgaaccg gtcccgagac ctacgatcat cccgtccttt acgaatttcg
    27901 cggcttcaat gccagctaat tgttttaaat tcatctttgc gtccaccttt ttttagtttg
    27961 agcatcgtgc tttcctcact attttagcgt gatttacggt aaaaagatac cattcttttt
    28021 tattccaata aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt ttttcattcg
    28081 tgatattcta acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg ctgactgttt
    28141 atcaaagcat atttgataag gatgcagaca ttgcatttat tttttatcac aaaaaaagaa
    28201 ccacctggat gtgtggaact agacgcaaat cgtgggaagg aggaaacaag gaatgcctac
    28261 aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta attcacctgc
    28321 tttgaacaaa ggatataaca gctttaagaa aactcaaact aacgtaaact cacctcaaaa
    28381 acgtggggta gcaactcgtg tgggtacaat gacacctaaa aaaccgaact cagctttacg
    28441 taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt actgcttata tcccagggat
    28501 tggtcataac ttacaagaac acagtgttgt attattacgc ggtggtcgtg taaaagactt
    28561 accaggggta cgttaccata tcgttcgcgg tgctttagat actgctggtg ttaacgaccg
    28621 taaacaaagc cgttctaaat acggaactaa aaaacctaaa gcttaatttt aattattaaa
    28681 caaatgaaac ttgcttctac tatatagaag taattactat gaacaatatt tcggaaggag
    28741 gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc agatcctatt
    28801 tataactcaa aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg aaaacgcggg
    28861 attgcagcta acattatcta taatgccttt gacttgatca aagaatctac aggtaacgat
    28921 ccattagaag tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga agttaaagca
    28981 cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag ttcgccctga acgccgcgtt
    29041 actctaggtc ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca cacaatggaa
    29101 gaacgcttag caaaagaaat catggatgct gcaaacaaca caggtgcttc tgttaaaaaa
    29161 cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta tcgctggtaa
    29221 gattctctct gtttggtcgg cttttataag tcgacctcag atagtgtcct tatttttagc
    29281 aactagatag taaagagagg agaaacaagg agaatgacca gagaattttc tttacaaaac
    29341 actcgtaata tcggtatcat ggcccacatc gatgctggta aaacgactgc tactgaacgt
    29401 atcttgtact atactggtaa aatccataaa attggtgaaa ctcatgaagg agcttcacaa
    29461 atggactgga tggaacaaga acaagaacgt ggtattacga tcacttctgc tgctactaca
    29521 gcacaatgga agggtcaccg aatcaacatc atcgacacac caggacacgt agacttcaca
    29581 gttgaagttg aacgttcatt gcgtgtactt gatggtgctg taactgttct tgatgcacaa
    29641 tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag caacaactta tggtgtacct
    29701 cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg acttcttata ttcagtatca
    29761 actttacatg accgtttgca agcaaacgct cacccaatcc aattaccaat tggtgcggaa
    29821 gacgacttca caggaattat cgaccttgta acaatgaagg ctgaaatgta tacaaacgat
    29881 cttggaacag agatcgaaga aacagaaatt cctgaagaat accgcgaatt agctgaagaa
    29941 tggcgcgaaa aattggttga agctgtagct gaaactgatg aagaacttac tttgaaatac
    30001 ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag gtatccgccg cgcaacagtt
    30061 aacgtagaat tctacccagt actatgtggt tcagcattta agaacaaagg ggttcaatta
    30121 ctattggacg ctgttcttga ttacttgcca tcaccgttag atatcccagc tatcaaaggg
    30181 attgacccta aaactgatga agaagtagaa cgtcctgcag acgacagcgc tcctttctca
    30241 gctttagcat ttaaagttat gactgaccca ttcgtaggtc gtttaacttt cttccgtgtg
    30301 tactctggtg ttttacaatc aggttcatac gtacaaaacg caacaaaagg caaacgtgaa
    30361 cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt cagaaatctc tgaagtttac
    30421 gctggagata tcgctgctgc tgttggtttg aaagacacaa ctactgggga cacactatgt
    30481 gatgaaaaga gtctagttat tcttgaatcc atggaattcc cagaaccagt tatccaagtc
    30541 gctattgaac ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt acaaaaacta
    30601 tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga aacaatcatc
    30661 gctggtatgg gtgaacttca cttagatatc atcgttgacc gtatgcgtcg tgaattcaaa
    30721 gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg aaactttccg tgctggcact
    30781 caagccgaag gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg tcacgtatgg
    30841 attgaattta caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc gatcgtcggt
    30901 ggggttgttc ctcgtgaata cattccagct gttgaaactg gtttgaaaga tgctatggaa
    30961 aatggtgtct tagctggcta tccattagta gatatcaaag ctaaacttta cgatggttca
    31021 taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat ggcattacgt
    31081 gcagcagcta agaaagctaa tccagtaatc cttgaaccaa tcatggctgt agaagttgtt
    31141 atccctgaag attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg tggacgtgtt
    31201 gaaggtatgg aagctcgcgc tggcggccaa caagttgttc gtgcgatggt tccacttgct
    31261 gaaatgtttg gttatgctac aactcttcgt tctgctactc aaggtcgtgg tacatttaca
    31321 atgacatttg accactacga agatgtacca aaatcagtac aagaagaaat cattaagaaa
    31381 aacggcggaa aagctgaata atttcttaaa accggatagt cctgcaaaga gattatccaa
    31441 agagactata tttttaatat agtttcgtgt aaaataatta tcgatgatat gggcatgtgg
    31501 tgtaccttgt gtacatcata tacctatcag acaaataaaa aatatattgt atttttagga
    31561 ggacatttta aaatggcaaa agaaaaattt gaccgttcta aaccacacgt taacattggt
    31621 actatcggac acgttgacca tggtaaaact acattaacag ctgcaatcac aactgtacta
    31681 tctaagaaaa acggcggcca agctatggct tacgatcaaa tcgatggtgc tccagaagaa
    31741 cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt atgaaacaga tacacgccac
    31801 tacgctcacg ttgactgccc aggacacgcg gattacgtta aaaacatgat cactggtgct
    31861 gctcaaatgg acggagctat cttggtagtt tctgctgctg acggcccaat gcctcaaact
    31921 cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt attcttgaac
    31981 aaagtagaca tggttgatga cgaagaatta ctagaattag ttgaaatgga agttcgtgac
    32041 ctattaacag aatacgaatt ccctggtgac gatgttcctg tagttgctgg atcagctttg
    32101 aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat ggctgcagtt
    32161 gacgaataca tcccaactcc agaacgtgac aacgacaaac cattcatgat gccagttgaa
    32221 gacgtgttct caattactgg acgtggtact gttgctacag gtcgtgttga acgtggacaa
    32281 gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc aaaaacaaca
    32341 gttactggtg ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg agacaacatt
    32401 ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt tttagctaaa
    32461 ccaggtacaa tcacacctca tacaaaattc tctgcagaag tatacgtgtt gacaaaagaa
    32521 gaaggtggac gtcatactcc attcttcact aactaccgtc cacaattcta cttccgtaca
    32581 actgacgtaa caggtgttgt tgaattacca gaaggaactg aaatggttat gcctggcgac
    32641 aacgtaacaa tggaagttga attaatccac ccaatcgcta tcgaaaacgg tactaaattc
    32701 tcaatccgtg aaggcggacg tacagttggt gccggtgtcg ttacagaaat caaagcttaa
    32761 ttcacttaag ttttaaagta aagcaacatt tattcggacg taatcgctcg gaacgaaggt
    32821 ctcaggatct ttgttccgag cttttttatt tattgcagga tcaggaaaaa agacaggagt
    32881 attataatag aaggaacgcg aaataaaaga gtcagtattt tctaaatgaa actttatgaa
    32941 aacaaaatgt tttttacaag aaaaagcctt gctttgtgag tttatatata ttataatgac
    33001 aaaggtgcgt ttactttagt gaattgcaca gacgtaatca gaatgatttc gtcggctaag
    33061 aaacgagagg ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac ggaaattttc
    33121 gtggagctat gtctactttt caaaataggc gaaggaggga acaagatggc aaaacaaaaa
    33181 attcgtatcc gtttaaaagc gtatgaacac cgtattttag atcaatcagc ggataaaatc
    33241 gtggaaactg caaaaagaac tggagctagc gtgtctggtc caattccatt accaactgaa
    33301 cgcagtcttt acacaatcat ccgtgcgact cataaataca aagattcacg cgaacaattc
    33361 gaaatgcgta cacacaaacg tcttatcgac attgtgaacc caacaccaaa aactgttgat
    33421 gctttgatga agctagactt accatctggt gtaaacatcg aaatcaaatt ataaaactaa
    33481 aacaaaaata atggaggtgt actcatgacc aaaggaatct tagggaaaaa agtgggaatg
    33541 acacaaatct tcactgaatc tggtgaatta attccagtaa ctgttgtaga agctactcca
    33601 aacgtagtac tacaagtgaa aacaatggaa acggatggct acgaagctat tcaagttggt
    33661 taccaagata tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt tgcaaaagca
    33721 aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg agaatatgaa
    33781 gtaggaaaag aaattacagt agatgttttc caagcaggag acattattga tgtaacaggt
    33841 actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag ccgcggacca
    33901 atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt tgcacctaac
    33961 cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt aactatccaa
    34021 aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa aggtaatgtt
    34081 cctggagcta aaaaatcttt aatcactatc aaatcagctg tgaaagctaa ataattgtag
    34141 gaagaaagga ggaactaagg aatgccgaat gtagcattat tcaaacaaga tggaagccaa
    34201 aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg aacctaatga aagtgttgtt
    34261 tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc ggttaaaaac
    34321 cgtagcgctg ttcgtggcgg tggacgtaaa ccatggcgtc aaaaaggaac aggtcgtgca
    34381 cgtcaaggat ctatccgctc accacaatgg cgtggaggtg gcgtagtctt tggaccaaca
    34441 ccacgttctt acagctacaa acttcctaaa aaagttcgtc gtttagcaat gaaatctgta
    34501 ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg aaggcttgaa cttcgacgca
    34561 ccaaaaacaa aagaattcaa acaagttctt gctaacttat ctatcgactc taaagtattg
    34621 gttgttctag aaaaaggtaa cgactttgct gctttatcag cacgtaactt accaaacgtt
    34681 tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg tatcaaatac aaaagtgtta
    34741 gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg cataatgaac ttactagacg
    34801 taatcaaacg cccagtgatc actgaaaaat caatgcttgc catggatgac aagaaataca
    34861 cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta gaagctgctt
    34921 ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc aaacgtatgg
    34981 gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat cgttacattg actgaagatt
    35041 caaaagaaat ccaattattc gaagctgctg aataagcact aatagataac tcattaaatt
    35101 aggagggaaa aacgtggcga ttaaaaagta caaacctacc tcaaatggtc gtcgtaacat
    35161 gacaggttct gattttgctg aaatcacaac atcaacacct gaaaaatcat tgttacaacc
    35221 attgaaaaac catgctggac gtaacaacaa cggccgcatc actgtacgtc atcaaggtgg
    35281 cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt aacaaagata acgttgttgc
    35341 aactgttcaa acgatcgaat atgatccaaa ccgttcagct aacatcgcgt tagtacacta
    35401 tgaagatgga gtgaaagctt acatcttagc acctaaagga ctagaagtgg gtatgaaact
    35461 tgtttcaggt ccagaagcag atatcaaagt cggaaatgct cttccattag aaaacattcc
    35521 agttggtact gttatccaca acattgaaat gaaacctggt aaaggtggac aattgatccg
    35581 ttctgctggt acaagtgctc aagtacttgg taaagaaggc aaatacgtat tgatccgctt
    35641 gaactctggt gaagttcgta tgatcttagc tgcatgccgt gctacaatcg gttctgtagg
    35701 taacgaacaa cacgaattaa tcaacatcgg taaagctgga cgctctcgtt ggatgcgtaa
    35761 acgcccaact gtacgtggta gcgtaatgaa ccctaatgat cacccacacg gtggtggtga
    35821 aggtaaagct cctatcggac gtaaagcacc agtttctcct tggggtcaac cagctcttgg
    35881 ttacaaaact cgtaacaaaa aagctaaatc agacaaactt attgttcgtc gtcgcaaaaa
    35941 taaataatta ctttgaccat gtgtcgcaca ttttgagagg aggttcacca tgggtcgtag
    36001 tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa aaagttgaag ctcaagcagg
    36061 ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt tcccaagttt
    36121 tgttggatat accatcgcag tttatgatgg acgtaaacat gtaccagttt acatccaaga
    36181 agacatggta ggacacaaat taggtgaatt tgcacctaca agaacttatc gtggacatgc
    36241 tgctgacgat aagaaaacga aacgctaatt tgaggggagg ataaatccaa atggcagaac
    36301 aaatcacatc agctaaagca actgcaaaaa cagttcgcac ttcacctcga aaagcccgtt
    36361 tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc ttgaaattca
    36421 caccaaacaa atctgctgga atcattgaaa aagtattgat gtcagcagtt gcgaatgcag
    36481 aaaataactt tgacttagat gttgaaaact tggtagtaag cgaagctttt gttaacgaag
    36541 gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca atcaacaaac
    36601 gtacaagtca tatcacggta gtcgtatcag aaaaataagg agggataatc agtgggtcaa
    36661 aaagtacatc caattggaat gcgtgtaggc atcatccgcg actgggatgc taaatggtat
    36721 gctgaaaaag agtatgctga gttcttacac gaagatttaa gaatccgtaa atttatttca
    36781 actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac aggtcgcgta
    36841 aacatttcaa ttcacacagc taaaccaggt atggttattg gtaaaggcgg ttctgaagtc
    36901 gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa gaatccatat caatatcgtg
    36961 gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc acgtcaatta
    37021 gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc aatgcgtgct
    37081 ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga tatcgctcgt
    37141 tcagaaggat actcagaagg aactgttcct cttcatactt tacgtgcgga cattgattac
    37201 gcatgggaag aagcagatac aacatacgga aaactaggag ttaaagtgtg gatctatcgt
    37261 ggagaaattc ttccaactaa aaaaaacact gagaaaggag ggaaataatc atgttagtac
    37321 ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa gctaaaggcg
    37381 gaaaagaagt agcattcggt gaatacggtt tgcaagctgt tgattcacat tggatcacaa
    37441 accgccaaat cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt ggtgggaaag
    37501 tatggattaa aattttccct cacaaatctt atactgccaa agcaattggg gtacgtatgg
    37561 gttctggtaa aggggcacct gaaggatggg ttgcaccagt aaaacgtggt aaaatcatgt
    37621 ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta gcttctcaca
    37681 aattaccaat gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa tcgaatgaag
    37741 gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa acaattaaaa
    37801 gaagaattgt ttaatcttag attccaatta gcaacaggtc aattagaaaa cactgcacgt
    37861 attaaagaag tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga acaagctaag
    37921 taattatgga aggaggccat taagcgtatg actgaaggaa gaaatcaacg caaagtttat
    37981 caaggtcgcg tggtttcaga caaaatggat aaaacaatta ccgttgctgt cgaaacaaag
    38041 aaaaaccacc ctatctatgg caaacgcatg aactattcta agaaatacaa agtgcatgat
    38101 gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta tggaaactcg tccattatca
    38161 gctacaaaac gtttccgttt gttggaagta gtcgaagaag cagttattat ctaataacac
    38221 gagattttcc tatatagatt gaatgcaaaa tctgaaagga ggatacacaa cgtgatccaa
    38281 gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt gacgatcaaa
    38341 gtccttggtg gatctggtcg taaaactgct aacatcggtg atgtgatcgt tgctacggtt
    38401 aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc cgttatcgtt
    38461 cgaacaaaat caggagctcg tcgtacagac ggttcatata ttaaatttga tgaaaatgct
    38521 gcagtgatta ttcgtgatga taaaagccct cgtggaacac gtatctttgg gccagttgca
    38581 cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga agttttataa
    38641 tcttgagaca cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa aggcgataaa
    38701 gttaaagtta tcactggaaa agacaaaaac aaagaaggcg ttgtactagc agcgttcccg
    38761 aaaaaagata aagttattgt cgaaggtgta aatgtcgtta aaaaacacca aaaaccttct
    38821 caagctgctc ctcaaggtgg aattgttgag atggaagcgc caattcatgt ttctaacgtg
    38881 atggtggttg actctactgg tgtagctggc cgagttggct acaaagaagt agacggcaaa
    38941 aaagtccgtg tttctaaaaa aaccggtgaa gttttagata aataaaagtt taaggaagga
    39001 gggtcttact gaatgaaccg cctaaaagaa aaatatctta aagaagtaac tccatcattg
    39061 atggaaaaat ttaactatag ctctgttatg caaacaccta aagttgaaaa aatcgtcatc
    39121 aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact tagataaagc agttgaagaa
    39181 ttaactttga tcactggtca aaaaccaatg attacaaaag ctaaaaaatc aatcgctgga
    39241 ttccgtttac gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg agaaagaatg
    39301 tacgaattct tagacaaatt ggtttctgtt tcactaccac gtgtacgtga cttccatggt
    39361 gtaagtaaaa aagcctttga cggtcgtgga aactatactt taggtattaa agaacaattg
    39421 atcttcccag aagtagatta tgatttagta gataaagtac gtggtatgga catcgttatt
    39481 gtaacaacag caaactctga tgaagaatct cgtgagttgt tgacacaatt aggcatgcca
    39541 ttccaaaaat aattaaagga ggcgaactac gtggctaaaa aatcaatgat tgctaaaaac
    39601 aaacgtcctg caaaattttc aacacaagct tatactcgtt gcgaacgttg cggacgtcca
    39661 cattcagttt atcgtaagtt ccatctttgc cgtatttgct tccgcgaact tgcctataaa
    39721 ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca taagaaggag
    39781 gtaaaagatc aatggtcatg acagatccaa ttgcagattt tctaactcgt atccgtaacg
    39841 caaacatggt taaacatgag tctttagaag tacctgcatc aaaaattaaa cgtgacatcg
    39901 ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa gatgacaaac
    39961 aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt atcacaaact
    40021 taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa gtaccaaaag
    40081 tattgaacgg tttaggtatc gcaatcattt cgacttctga aggtgttgtc actgataaag
    40141 aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa tcaagtagaa
    40201 tctacaagga ggtgtcttta agtgagccgt atcggaaata aagtcgttgt tatccctgaa
    40261 ggagtaacag ttactcaaga cggaaacaac gttacagtta aaggaccaaa aggtgaattg
    40321 actcgtactt tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt aactttcact
    40381 cgtccaaacg acagtaaaga aatgaaaaca attcatggaa caactcgtgc gaacttcaac
    40441 aacatggttg taggtgtcag cgaaggtttc caaaaagcgt tagaactaat cggggttggg
    40501 taccgtgcac aattacaagg atcaaaactt gtattgaacg ttggttactc acatccagta
    40561 gaaatcacac caccagctgg agtaactgtt gaagtaccag ctaacacaca agtaattgtt
    40621 aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa acattcgtgg aactcgtcct
    40681 ccagaacctt ataaaggtaa aggtattcgt tatgttggcg aatatgtacg ccgtaaagaa
    40741 ggtaaaactg gtaaataatc gtttagaaaa attgcataga agccagcagc aggaaaaagg
    40801 catttggctg aagtttacct tcaaaaaaat acatttttac ttgtgccgca gcttctattt
    40861 ttcgaaacgt atttactgct tgaaatagta gtacaataaa aataaagagg tgacaattgt
    40921 gattacaaaa ccagataaga acaaaacacg tcaaaagaga catcgtcgtg tacgtaacaa
    40981 tatctctggt actgctgagc gcccacgctt gaacgttttt cgttctaaca aaaacatcta
    41041 cgctcaaatt attgatgacg tagcgggtgt aacgctagca agtgcctcta ccttggataa
    41101 agaaatttca ggtggaacaa aaacagaagc agcacaagct gtcggtaaac tagttgctga
    41161 acgtgcagct gaaaaaggca ttaaagtagt agtctttgac cgtggtggat acctttacca
    41221 tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat ggactagaat tttagaagaa
    41281 ggaggaatac cattcatggt ttatatcgat ccaaaacatt tggaattaga agaccgcgtt
    41341 gttgcaatta accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg ttttgctgct
    41401 ttagttgttg tcggtgacaa aaacggacac gtagggtttg gtacaggtaa agctcaagaa
    41461 gttcctgaag caatccgtaa agcaattgaa gatgcgaaga aaaacttagt tgaagtacct
    41521 atggttggtt caacaatccc acacgaagta atcggtgctt ttagtggtgg tagaatccta
    41581 atgaaacctg ctgtagaagg ttctggggtt gctgctggtg gaccagttcg tgccgtatta
    41641 gaattagctg gggtagcaga tatcacttct aaatctttag gctcaaatac accaatcaac
    41701 gttgttcgcg caacagttga aggtttgaaa caattgaaac gtgcagaaga agttgcagaa
    41761 cttcgcggca aatcagttga agaattaatc ggataaggag gacaacaata atggctgaat
    41821 taaaagttac tttaaaacgc agtattatcg gacgtcctca aaaccaacgc gatacagtta
    41881 aagcgttagg tcttacaaag attaacagtt cagtggttaa gccttctaat gaagcaatta
    41941 aaggcatgat caatactgtt tctcacctag tggacgttga agaaatttaa tttagaaaaa
    42001 tcataactag aacattaagg aggtgccaaa cctatgaaac ttcatgaatt aaaacctgct
    42061 gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg taacggaaaa
    42121 actgccggac gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt gcgtttagga
    42181 tttgaagggg gacaaacacc attgttccgt cgtttaccaa aacgaggatt cacaaacgtg
    42241 aaccgtaaag actatgcagt cattaatctt gatgtattga accgctttga agacggagct
    42301 gaagtaactc ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa agctggaatc
    42361 aaagtattag ctaatgggga actaaacaag aaattaactg tgaaagcagc aaaattctct
    42421 aaagcagctc aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat ctaatgttta
    42481 gactattaaa agatgctttt aaagtaaaag acattagatc gaaaatccta tttactgtat
    42541 ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt accaggcgta aatgctgctt
    42601 cattgcaaaa cctttcttct ttgccatttt tcaacatgtt gaacttggta agtggtagtg
    42661 cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc atacattact gcttctatcg
    42721 tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg aaacaagggg
    42781 aagttggtcg taaaaaactg aatcaagcga ctcgatatct gacattagta ttggcattta
    42841 tccaatctgt cggtatcact gctggattca atcaatacgc tcaattaggt ttcgtgaata
    42901 atcctaacgt agcaacttac gtaacgatcg gtttgattct aacagccggt acgatgtttg
    42961 ttacttggct aggagaacag atcacagaaa aaggtatcgg aaatggcgta tcaatgatca
    43021 tttttgcagg tatcatttct agattgcctg aaagtatcag agaaatttat gaagattact
    43081 ttataaatat tgatccttca gacatatgga aatctgctat ttttgtggcc attttactag
    43141 tagcggtctt gttgatcgtg acattcgtaa catttttcca acaagctgaa cgcaagatcc
    43201 cgatccaata tacgaaacgc gtagctggcg cgcctacaag cagctatctg ccgttgaaag
    43261 taaatgcagc tggagttatt ccagttatct ttgctagttc gtttattgct acgccaaatg
    43321 ctgttttaca agcattgagc ggtagattcg gttctgaaaa ctggtacaaa gtgatgatgg
    43381 agatctttaa ctataataca gcacctggtg ctacgattta tacgattctg atcgttgcct
    43441 tcactttctt ctacgccttt gttcaagtaa accctgagaa attagcggaa aacttgcaaa
    43501 agcaaggaag ttatatacca agcgtacgcc ctggtaaggg gactgaagag tatgtctcaa
    43561 gattactgat gcgtttaagt acagttggtg cagtcttcct tggtttagtg gcattgcttc
    43621 caatcattgc tcaaatggtt tgggatcttc cacaatcaat cggattaggc ggtacaagct
    43681 tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc ttaatgctga
    43741 aacgtaaata tgttggattt attgagtaag aaagcgtgtt gaggtttccc ctcaacacac
    43801 gttctcacac atttattagg agggaaaaca atgaacctca ttttaatggg gctgccaggc
    43861 gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt atggtattcc gcacatctca
    43921 acaggagata tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct ggaagcaaag
    43981 tcttatatgg ataaaggtgc attagttcct gatgaagtaa caaatggaat cgtgaaagag
    44041 cgactagctg aaccagatac agagaaaggc ttcttattag atggctttcc acgtacgcta
    44101 gatcaagcaa aagctctgga cgcaatgcta aaagatttga ataaaaaaat tgatgctgtc
    44161 atcgatatcc atgttggtga agaaatcttg gtcgaacgtc tagccggccg atttatctgc
    44221 agcaattgtg gagcaactta ccataaaatt ttcaacccta caaaagttga agacacatgt
    44281 gatcgttgcg gtgggcatga attctatcaa agagaagatg ataaacctga gacggttaaa
    44341 aatcgtctgg ctatcaacat caaaaatagt gaaccaatct tggcttatta taaagaacaa
    44401 ggtttgctaa acactattga tggtgctcgt gaaatcgatg ctgtatttgc agatgtacaa
    44461 aaaatcattg aaaaatagca cgattgcccg tagccgactt gcattatgca gttactcttg
    44521 tcaagttcaa taaattatga tagaatatgt cagtccgact tctagagaat cctctagatg
    44581 atccattttg agaattttga ggtgggaact gcagtttccg ccattttatc gtgtgaaaat
    44641 gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg aagtcgaagg
    44701 tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa acggacacca
    44761 agttcttgct actgtttcag gtaaaattcg tatgcattac atccgtatct tacctggtga
    44821 caaagtaacg gttgaattgt ctccatacga tcttaaccgt ggccgtatta cttatcgctt
    44881 taaataattg tactccgtaa aactacagga ggtattatca tgaaagtaag accatcagta
    44941 aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat ggtgatttgc
    45001 ccagcaaatc caaaacataa acaacgtcaa ggataattgg aggtgctata aatatggctc
    45061 gtattgcagg agtagatatt cctcgtgata aacgtgtagt agtttcactt acttatatct
    45121 atggtattgg taacactact gctaaaaaga tcttagctga tgctggtgta tctgaagatg
    45181 ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa gtagataaat
    45241 taaaagttga aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt ttgatggaaa
    45301 tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga caaaacacga
    45361 aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat cgctggcaag aaaaaataat
    45421 tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt aaacgccgtg
    45481 tgaagaagaa tatagaagca ggtattgcac atatccattc tacattcaat aacactatca
    45541 tcatgatcac tgatactcac ggaaatgcat tagcgtggtc atcagctggt tcattaggtt
    45601 tcaaaggaag caaaaaatca actccttttg ccgctcaaat ggcagcagaa actgctacaa
    45661 aagcagcaat ggaacacgga ttaaagactg ttgaagtaac agttaaagga cctggttctg
    45721 gacgtgaagc agcaattcgt tcattacaag ctacaggttt agaagtgact gcaattcgtg
    45781 acgtgactcc agttcctcat aatggtgccc gccctccaaa acgccgtcgt gtttaatgag
    45841 tgccgctcat tttgagtttt atcagtaacg tcattgaaca agacactttt cgttttgaaa
    45901 ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat tgatgaagaa
    45961 aaagattatg gcaagttcat cgttgaacct cttgaaagag ggtatgggac tactttaggg
    46021 aattccctac gtcgtatttt attatcttct ttgccaggtg ctgccatcac taatattcaa
    46081 atcgatggtg tactgcatga attctctacc atcaaaggtg ttagagaaga cgtcactcaa
    46141 atcatcttga acattaaagg cttagcgctg aaaatgtatg gccaagaaga aaaaaccctt
    46201 gaaatcgata ttaccggacc tgctacagta actgctggcg atattatcgt tgatagtgat
    46261 gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc tacgttccat
    46321 gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa taaaaaagaa
    46381 gatatgccaa ttggcgttct tccagtcgat tcaatctaca cacctgttcg tcgtgtgaac
    46441 tatcaagttg agaacacacg tgttggacac cgtgaagatt ttgataaatt gacgatggag
    46501 atttggacag atggttcaat tgaaccgttg gaagcaatga gtttgtctgc gaaaatcatg
    46561 actgaacatt tggatatttt tgtgaactta actgatgaag caaaaaatgc tgaaatcatg
    46621 gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga agagttagac
    46681 ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt acaagaactt
    46741 acaaataaat ctgagcctga aatgatcaaa gtccgtaact taggacgtaa atcattagaa
    46801 gaagttaaag caaaattgca cgacttaggt ttaggtttac gtaaagaaga ttaaaacctt
    46861 ttacgaagga gggaaaccaa cgtgagttat cgtaaactag gacgcacatc tagccaacgt
    46921 aaagcgatgt tgcgtgattt aacaactgat ttattaatca acgaacgtat tgtgactact
    46981 gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt aggtaaacgc
    47041 ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt tgccagcgtg
    47101 cgtgaagaaa atgaggacat cgtcattgaa tcagctttac aaaaactgtt caacgatatt
    47161 gcacctcgtt acgcagaacg tcaaggcgga tacacacgca ttttgaagac agaaccaaga
    47221 cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct aaaatcacac taataacatc
    47281 actttataga tgattctttt agagtgttat gatgttggaa gatatttttc cgagtctagc
    47341 tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag cactcatcat aaagtgtttt
    47401 tttatacctt caattacctg tcatgacaga tatgtttgaa ggcataaaaa aacattttaa
    47461 gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac tttaggatga
    47521 agaaggagta atcatggagc caattattga attagaaaag ataaattata aataccagcc
    47581 agatgatctt cgccccgcat tgaaagatgt ctcctttaca attgataaag gtgagtggat
    47641 cgctattatt ggacacaatg gttcaggaaa atcgacactt gccaaaacga tcaatggatt
    47701 gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa atattggatg aagaaaatat
    47761 ttggacgatc cgacagatgg ttggaatggt ttttcaaaat ccagacaatc agtttgttgg
    47821 ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat caagggattc ccagagaaga
    47881 aatgctagtg cgtgtgaaag atgcacttga gaaagttaga atggctgagt ttgcttcacg
    47941 cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt gccattgccg gcgtggttgc
    48001 cttacgacct gatatcatta ttttagatga agcaaccagt atgctagatc cagaaggtcg
    48061 tgaagaagtg atctctacga tcaagaagat caaagaagaa agtcaattga ccgttatatc
    48121 tatcactcat gatattgatg aagcagctaa tgccaatcgg atactagtga tgagacaagg
    48181 cgaacttgtc cgtgaaggta caccaaaaga aattttttct gctggtcctg aactgatcga
    48241 cttaggtcta gatctaccat ttcctgaaaa actgaagagc gcactgaaag aacgtggggt
    48301 tgatgtgccc agtgaatata tgacagaaga aaggatggtg gattggttat ggacatccgt
    48361 tttgaacaag tagacttcac ttatcagccg aatacaccat ttgaacaaag agccttattt
    48421 gacatcaata tgacgatcaa agaaaacagt tatactgcat tagtagggca taccggaagc
    48481 gggaaatcaa ccttacttca gcatttgaat gcgcttgtta agccaacaag cggtacagtc
    48541 catattggag aacgagatat tcagccggat acggataata agaatttaaa gcccatccga
    48601 aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc tattcgaaga aacggtagca
    48661 aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc actagtccta
    48721 gcaaaagaaa cattagaaca agttgggctg gatgaaagct atttggaacg ttcgccattt
    48781 gaactttcag gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc catgaggccg
    48841 gaagtacttg tattggatga accaacggca ggacttgatc cccagggcag aaaagaaatg
    48901 atggagatgt tttggcgtct tcataaggaa cagcagatca ctattgtatt agttacgcat
    48961 ttaatggatg atgtagccaa ttttgcagat tatgtctatg tgttggaaaa gggacgaatc
    49021 gttaatagcg gggagccgca agaggtcttt caaaacattg aatggctaaa agaaaaacaa
    49081 ctaggcgtac ctactgccac cgagtttgca gaagaattaa tggctaaagg gatgaacttt
    49141 gctaccttgc ctttgacagc tgaagaatta gcagacgcta tcgtccatta tgctggagga
    49201 aatgcccatg atgaataaat tgatatttgg tcgttatatg cctggagatt ccttgatcca
    49261 tcgattggat ccccgagcta aattattagc cagtttttat tttatcggaa tcattttttt
    49321 agcaaataat tgggaaacgt accttttgtt ggcatttttc acattacttt ctgtcttctt
    49381 gtcaaaagta gatttgaaat tttttatccg aggcattcgc ccattgatct ggctaatcct
    49441 cttcactgta gctttgcagg tcttgtttac aagtggcgga gaaacttatt ggagttgggg
    49501 tgttttccat atcactgaat ttggtgtgat gaacggcctc tttattttct gtcgattcgt
    49561 gttgatcatt tttatgtcga ctctactcac gcttactaca ccgcctttgg aattatcgga
    49621 tgcgattgag tacttgcttc gtccattgaa agccgtacgg ttcccagtcc acgaaatttc
    49681 attgatgctg tcgattgctc ttcgttttgt gcctacattg atggacgaaa cagaaaagat
    49741 catgaacgct cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag ttcagaaaat
    49801 gaaagcagtc attcctttgc tgattccttt atttgtcagc agcttcaacc gtgcggaaga
    49861 tcttgcgact gctatggaag caagaggcta caaaggtggg gaaggaagaa cgaaatatcg
    49921 tgtattgcat tggcataata gagacacgat ggttatcatt gcttttgtat tattaactgt
    49981 gctactgatc tttttaagag gataaacgtt cagtgggccg ctatctattc ttttcttgct
    50041 gatctgtttg caagaaagga tgcaagcagt gtatgacaag aaatattagg gtctgtggca
    50101 gactaaggtc acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa attttataga
    50161 aaaatcatta ttccagataa ccttttagga acaaaggaga gaaaaacatg gtacgttaca
    50221 aagcaatcat tgcgtacgac ggaacaaatt ttaatggatt ccaaaaacag ccaaatgggc
    50281 gtactgttca agaagaagta gaaaaaacat tacaaaaaat ggcaaatgga aaagaaatca
    50341 cggtctttgg ttcgggaagg acagatgccg gagtacatgc gatcgggcaa gtaatccatt
    50401 ttgattatcc agaggagcgt ccgttagaac gaatgcgttt cgcgctagat acccagtcac
    50461 cagaggatat tgctgtaaga caagtagaaa ttgtttcaga agacttccac gcaagatatt
    50521 tggtcaaaga aaagacgtat caatttcgag tggatatagg gaaaccacgc agtccttttc
    50581 gacgccatta tgcaagttat tttccttacc cacttgattt ggaaaaaatt cagcgggcgc
    50641 ttcctgattt attagggact cacgatttca cctctttttg tgcatcagga agctcgatag
    50701 aggataaaat acgtacgatc tacgaggcta agatggaggt aaatgaaaca aaagacgaat
    50761 tgttgtttac atttagggga aatggcttct tgtataagat gatacggatc ttagtaggaa
    50821 cgcttttgaa aattggcaat ggccgattgc ctgaagatag tatcccagat attattgcga
    50881 aaaaagatcg taatgcagca ggacctacag cacaccctga aggactttat ttgtatgaag
    50941 tgaaatatga ataagaggtt atgatagaag tggtcagctt caagaaataa gaaggtattt
    51001 acgaaaatta cttctcaaaa ttttgcaaaa tttcggctta ttttcgaaga agctacttct
    51061 ggaacaccgt ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc acaaagagca
    51121 atttcaaaaa acagcttctc atctttgatg aatatcgtga cttgtggacg aggcgttgat
    51181 aagtgaatca tctttttgtt acctattttt tctgtaacca aaaaacgatg gaacgttgtt
    51241 ttgattgccc ttggtgcgga cagtctgaac aggttccatc gtttctttat tttctacgtc
    51301 ttcttctgtg tgtgtttcgt ggttgttctt ctattgctgg atcaatcgtg ccataaaagg
    51361 ggcgattgcg gtcaaggtac cagacataaa gaataaacaa tgcaatacat aggacaaaaa
    51421 ggattgcacc tatcgtaaat ccttcaggca agtaactgaa agtgacagta tgtttacctg
    51481 ctggaacttt tatcatcaag aatgcgtctt tgaaattgct gaccttgact ggtttcccat
    51541 caattttagc tttccatcct ttgtcataag gaatagttgt caccaacatg gaatcttcag
    51601 cggtatttac agtcccagaa gcttttctgc cagtggcttc catttcgaca cctttttctt
    51661 tgatctgatt cattgcggct tgataggcta caacatctag accaacgact ttaggctcca
    51721 tgaaacttat ttctttcgtc ccataaaaac ttgcagtaaa tgtcactgtt gtctcttgag
    51781 gataatagcc aagatcatag tattgaccag aaatgttgat ttgcgttttt cgcgatgtac
    51841 cattgacagt gatcgtcgca ctagaacttt ctaattgcgc aaaattggtt gggaacaaac
    51901 ttaagtaagc ttgtgtattt gcaggcacat ggacttccca agtgagctct tttggctggt
    51961 tatctgctgt ttctgagtaa gtgacgccag tcgttgtctt tgtgatcgtc gtattcaact
    52021 gattcttcaa agtaggtgta tagaatgtaa aatactgttc atttagacca gacaatgtat
    52081 tgatcaagtt tgtctgactg gtcaaattat ctgtcactgg ctgctcaaca tcattaacgg
    52141 acaacggtgc agtaaatgct agtggcaatg cattgacatt ctgatacagt gtatagtcgc
    52201 cgctttgacc agcttggcgg aagccatatt tactgaacgt gctttcgttt ttagaaatgt
    52261 tatatttgat tcctgtaaac ccatccatca gcaaagtatt attgttataa cggatattca
    52321 aatttgtccc tcttgaacgg aaacctaatt tatccaggta agaggaagaa ttacgatttc
    52381 ttatagaaga aaacaaactg ataccactgt aaccatagtt gatcgaatcg tttgcagata
    52441 ctggatccag attttccaag cggtaaaacg agtcggaatc tttttttgtt tcatttacaa
    52501 gagctttcaa gtcaggataa ggatcagtat ataaactgcg ggaagcataa ttccaatcat
    52561 caagaatgcc atggatcata ctgttcgtat tgaccagtgc ttcagcagac atgatcaata
    52621 ataacaatat actcagatga ttgaatgaaa gttttttatg ctgataaaat cccacagcaa
    52681 aagcataaat ccctaagaaa accactgtca atacaaagga agtcatcgtc acataggtgt
    52741 agcttccttc aggtttgatg ccccatgcca ccgcaaagag agcagctacg accaaaatga
    52801 ttcctaacaa cagcccacga tcgtcttgtt ctatcttttc ccatcctttt cctgcaagca
    52861 ggagcactaa gaaagagaac agataggcat aacggaataa aaacatattc ggtgcatgca
    52921 taccatgcca gaacaaattc aatggaacaa gataaaaact tgcgataagt atggcgaaaa
    52981 gactgccgta caaaaattta tttttcaacg gtactttccg actggcaaaa taaaacagac
    53041 agaggactaa tggtagcaga cctgcgtaaa cgaacggaat cgatccgtat tttgtcgtgt
    53101 cataaacacc aatcatattt ttcatgatta gatccagata agaagtcgct tctgttttca
    53161 acgtagtgat ctgactcagt gcctcgccat ttgaccgaag atccagcact gctggcaata
    53221 caatgatcat tgaagcgccg cctgccagta aagaagtgat accataagga acaatcgttt
    53281 ttttatattc tgaccagttt gtcaatagac gggcgataaa atataagaaa gaaaatacgc
    53341 caatcataaa acccatataa aaactggata aaaacaagag taaataactg ataaataaaa
    53401 gtttcggccg tttaagatcc ataacacgat gaatccccaa aatcactaat ggaagataaa
    53461 caaatgcatc cagccacatg atgatctccg aatgtgcagt gataaatgag atcaatgcgt
    53521 aactgacgct aagtgcaaca tgcccccagc gaggaatctt gaacgtacgt gcaagaaacc
    53581 aaaaagccaa tccggcacag ccgaccttaa gcaaagtcag aacatacagt gcatctggca
    53641 tcatctggtt tggaaaaaag aacaccagtg gcgtgaagat cccgcctaga tagtaagaaa
    53701 ttaaagataa ataattcaat cctagagaag cattccatgt ataaaaaata ctttgctttc
    53761 caagaagaac atttcggaag ctggcatgaa aattagaaaa ctgtgaaaag gcatcacttg
    53821 ccaaaatact tctgctgctt ccaggataga ttccgatact tagataaaca agtgctaaaa
    53881 tcaaaaacgg aatcaggaaa ctagctgctg cgtagc
//
LOCUS       DASOUD010000007        45864 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.007, whole genome shotgun sequence.
ACCESSION   DASOUD010000007 DASOUD010000000
VERSION     DASOUD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.007"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(42..1052)
                     /locus_tag="U4A29_000424"
     CDS             complement(42..1052)
                     /locus_tag="U4A29_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO0679574.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1232..1576)
                     /locus_tag="U4A29_000425"
     CDS             complement(1232..1576)
                     /locus_tag="U4A29_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO0679575.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1569..2003)
                     /locus_tag="U4A29_000426"
     CDS             complement(1569..2003)
                     /locus_tag="U4A29_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO0679576.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2141..2878
                     /locus_tag="U4A29_000427"
     CDS             2141..2878
                     /locus_tag="U4A29_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0679577.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2871..4082
                     /locus_tag="U4A29_000428"
     CDS             2871..4082
                     /locus_tag="U4A29_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0679578.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4286..5068
                     /locus_tag="U4A29_000429"
     CDS             4286..5068
                     /locus_tag="U4A29_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO0679579.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5138..5800
                     /gene="trmB"
                     /locus_tag="U4A29_000430"
     CDS             5138..5800
                     /gene="trmB"
                     /locus_tag="U4A29_000430"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO0679580.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5898..7652)
                     /gene="ade"
                     /locus_tag="U4A29_000431"
     CDS             complement(5898..7652)
                     /gene="ade"
                     /locus_tag="U4A29_000431"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEO0679581.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7784..8713
                     /gene="tadA"
                     /locus_tag="U4A29_000432"
     CDS             7784..8713
                     /gene="tadA"
                     /locus_tag="U4A29_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEO0679582.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8670..9719
                     /locus_tag="U4A29_000433"
     CDS             8670..9719
                     /locus_tag="U4A29_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEO0679583.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9716..9994
                     /locus_tag="U4A29_000434"
     CDS             9716..9994
                     /locus_tag="U4A29_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEO0679584.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9969..10439
                     /locus_tag="U4A29_000435"
     CDS             9969..10439
                     /locus_tag="U4A29_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEO0679585.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10456..10758
                     /locus_tag="U4A29_000436"
     CDS             10456..10758
                     /locus_tag="U4A29_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679586.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10775..11146
                     /locus_tag="U4A29_000437"
     CDS             10775..11146
                     /locus_tag="U4A29_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEO0679587.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11118..11477
                     /locus_tag="U4A29_000438"
     CDS             11118..11477
                     /locus_tag="U4A29_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679588.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11649..12656
                     /locus_tag="U4A29_000439"
     CDS             11649..12656
                     /locus_tag="U4A29_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO0679589.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12681..13865
                     /locus_tag="U4A29_000440"
     CDS             12681..13865
                     /locus_tag="U4A29_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO0679590.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14302..15885)
                     /locus_tag="U4A29_000441"
     CDS             complement(14302..15885)
                     /locus_tag="U4A29_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO0679591.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16463..16606)
                     /locus_tag="U4A29_000442"
     CDS             complement(16463..16606)
                     /locus_tag="U4A29_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEO0679592.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16829..17308)
                     /locus_tag="U4A29_000443"
     CDS             complement(16829..17308)
                     /locus_tag="U4A29_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO0679593.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17381..17665)
                     /locus_tag="U4A29_000444"
     CDS             complement(17381..17665)
                     /locus_tag="U4A29_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679594.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17743..17937)
                     /gene="ssrS"
                     /locus_tag="U4A29_000445"
     ncRNA           complement(17743..17937)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U4A29_000445"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18009..19295)
                     /locus_tag="U4A29_000446"
     CDS             complement(18009..19295)
                     /locus_tag="U4A29_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO0679595.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19433..20104
                     /locus_tag="U4A29_000447"
     CDS             19433..20104
                     /locus_tag="U4A29_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEO0679596.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20070..20558
                     /locus_tag="U4A29_000448"
     CDS             20070..20558
                     /locus_tag="U4A29_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO0679597.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20571..21518
                     /gene="prmA"
                     /locus_tag="U4A29_000449"
     CDS             20571..21518
                     /gene="prmA"
                     /locus_tag="U4A29_000449"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO0679598.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21519..22283
                     /locus_tag="U4A29_000450"
     CDS             21519..22283
                     /locus_tag="U4A29_000450"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO0679599.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22516..24729
                     /locus_tag="U4A29_000451"
     CDS             22516..24729
                     /locus_tag="U4A29_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO0679600.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24740..25186
                     /locus_tag="U4A29_000452"
     CDS             24740..25186
                     /locus_tag="U4A29_000452"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO0679601.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25357..26616
                     /locus_tag="U4A29_000453"
     CDS             25357..26616
                     /locus_tag="U4A29_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEO0679602.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26705..27652)
                     /locus_tag="U4A29_000454"
     CDS             complement(26705..27652)
                     /locus_tag="U4A29_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO0679603.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28046..29338
                     /locus_tag="U4A29_000455"
     CDS             28046..29338
                     /locus_tag="U4A29_000455"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO0679604.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29353..31122
                     /gene="aspS"
                     /locus_tag="U4A29_000456"
     CDS             29353..31122
                     /gene="aspS"
                     /locus_tag="U4A29_000456"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO0679605.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31231..32106
                     /locus_tag="U4A29_000457"
     CDS             31231..32106
                     /locus_tag="U4A29_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO0679606.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32372..34255
                     /locus_tag="U4A29_000458"
     CDS             32372..34255
                     /locus_tag="U4A29_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO0679607.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34330..34614)
                     /locus_tag="U4A29_000459"
     CDS             complement(34330..34614)
                     /locus_tag="U4A29_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO0679608.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34604..35338)
                     /locus_tag="U4A29_000460"
     CDS             complement(34604..35338)
                     /locus_tag="U4A29_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO0679609.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35467..36090
                     /locus_tag="U4A29_000461"
     CDS             35467..36090
                     /locus_tag="U4A29_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO0679610.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36282..37403
                     /locus_tag="U4A29_000462"
     CDS             36282..37403
                     /locus_tag="U4A29_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEO0679611.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37410..40538
                     /locus_tag="U4A29_000463"
     CDS             37410..40538
                     /locus_tag="U4A29_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEO0679612.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LIKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40557..43751
                     /locus_tag="U4A29_000464"
     CDS             40557..43751
                     /locus_tag="U4A29_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEO0679613.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43897..44604)
                     /locus_tag="U4A29_000465"
     CDS             complement(43897..44604)
                     /locus_tag="U4A29_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO0679614.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            45369..45767
                     /gene="spxA"
                     /locus_tag="U4A29_000466"
     CDS             45369..45767
                     /gene="spxA"
                     /locus_tag="U4A29_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO0679615.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        14770 a         8095 c         9572 g        13427 t
ORIGIN      
        1 aaaaaaagcg agacataagt cttcgctttt tttgttttct tttattttga tgaatcagtt
       61 gtttcttcag ttgaagattc ttttgttgaa tcagttgatt ttgtgtcaga actctttgtt
      121 gaagctgtag tttcacttga gtctttcgta cttgatgaag ttgttgtgaa tgcactcaac
      181 acattttcaa aggcatcatc tttgattttt acatttgcat cttttaattc ttcaccgatt
      241 actttagttg tgaatgcatt gtcacttagt ttagtttcag tagcgatatc ttttagttga
      301 tctttgtatt tatccatgtc attgccttta ttttggtttt tcaccatttt gacaacgtag
      361 tattctgtcg cataagaagt aggatttgtt gtagtaataa catcggagat ctcaccgtct
      421 ttcaatttga aagctgcttc ttttacttct gcaggaatag ttgttgtcgt tgagtcgaat
      481 ttaactttac cgccatcttc ttttgtctct gtatcagttg atttgtcttt ggctaattta
      541 gagaaatcat ctccatcatc cgctgatttt ttcacatctt ttgcttcatc ttcacttgat
      601 aatttgatga tttgtgcttc tacttctgga tggaaagatt tccaagcagt tttcaaatcg
      661 tcatctgtaa tatctacgtg agctttcaag ccagcttcaa aagctaaatt gttacgaata
      721 tattctttat aagtatcttt cgtatatcca gcagcttcta attgtgattc aaaagtatca
      781 cctaatgatt tcgcttgttt gtcgtattct gcatccactt gcttatcggt tactttatcg
      841 ccatatttgt tattgaatac tttataaatg atcatccgtt gtactaagga ttggttcgat
      901 gattcaagtt ttgcttcatc atagaaatca gatacagtga tcgttccgcc tttcatcgtt
      961 gcgatatctt tattagtatc gcctgaacaa gctgctagag ttaaaacagc caacgcacta
     1021 gtagctgcca aaataattga tttttttttc attaaaattt gcactcctgt aattagttat
     1081 tatttttata tattaactat accatatccc atttccttta aaaacgttgt cgtggtagtt
     1141 tcaaacaaaa ttcaaaattt catcacattc ctaggaaata cgcaaacttt tctcacttat
     1201 atagttaaat ctgttgagaa aataatcatt attatatttg ttcaggatac tctggcagat
     1261 cagcttttag tttttctact tgttcattga tttgtgtgat acgtggttcg gcttggaatt
     1321 taaaatcttc gatatctttt ttgattgctt cttggaaagc aggaatcaca gattcagctg
     1381 tttcttttgt ttcgatcaat gcgcttttaa aatgattcag gctgttattt aaatcatttg
     1441 tcagagttgt tgcttcttcc aattcttcga tcagtttctt tcttgtttca ttcccgctcc
     1501 tgggcgccaa taacagacct ccgacagtgc cagcagcagc tccaaataac agtcctttga
     1561 aaaagttttt aatcatcgca ggacacctgc tttacgattg tttcttgaat agcttttagt
     1621 tcttccgatc cataactatc ttgatgattg acaaagtgaa tagaaaaatc atcctctttg
     1681 ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag attgataggc tagttcacgg
     1741 ttgttattca agatattaag acctttcatt tctggaaaag ctttttccaa tgcttgagca
     1801 atcttaggca cacgcgaaaa aacctctcct gcaagttcgg gatcatattc aaaaatatct
     1861 gctacatgac gtttaggcac aactaatgtg tgtcctttcg ttgcttgaga gatgtctaaa
     1921 aaagcataga ccttgtcgtc ttcatatatt ttatagcttg ggatttcttg attgatgatt
     1981 ttgcaaaaaa tacagttttc catataaaac atcctttcta taaagaataa atacttcagc
     2041 ttaactttac cataagttaa agctattgta aggaaaaaaa caaaataccg tcattttctt
     2101 cgtggtataa taaataaaaa taaatggcgg aggaaaaaga atgagtttaa ttatcgatca
     2161 tgtcacaggg gggtacggac atctccctgt gttaaaagat atcagtttcg atgtacagcc
     2221 tggagagata gtagggttga ttggactgaa cggagcaggg aaaagtacga ccatcaagca
     2281 cattatcggt ctgctggaag cttcaaaagg aacgatagaa atcaatcatc tatccttaaa
     2341 gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt ccagagactc cttctttata
     2401 tgaagaattg acattgagag aacatatcga agtcactgcg atggcctacg atatacctat
     2461 ggatatagca atgaaaagag cagaaaagtt gttggagact tttcggttat ctaatcgtct
     2521 tgactggttc ccagctaatt tttcaaaggg aatgaaacag aaagtgatga tcttatgcgc
     2581 ctttttgatc gagccttctt tatatatcat cgacgaacca tttttaggct tggatccact
     2641 agctatcaat gcattgcttg aattgatggt agaaatgaaa aaagaaggag ccggtatttt
     2701 gatgtctacg catattttag ctactgcaga aaaatattgt gatcgttttg tggtcttaca
     2761 taacggagag atacgagcag acggttctct tgagatgttg agaaaagaat ttcagctgcc
     2821 ggattcttct ttggacgaaa tctatattgc tttaacaaag gagcaggcac atgagtagtt
     2881 tttttcaaaa acgtttagca agacatcaaa agaaaatgat gaaatatatg cgttatgtat
     2941 tgaacgatca ttttgtttta gtttgtcttt tcttgattgg cggtgtggga ttatattatt
     3001 ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg agggctcata atctctgtat
     3061 tttggatgct ctgtttattc gtgggtaaga tggctacttt tgcagaggca gcagatattg
     3121 tttttttact tcctaaagaa aaagaaatgc aaacctattt ggaacgagct tttcgctatt
     3181 cttgcatttt tccgttgatc tttttgatat tggcatgcgg attggccatg ccattagtgg
     3241 tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta tctgatcata ttatggagtt
     3301 tgaaattcag tcatctgcaa atcaagagag cagaattgtt tcgactagat caacaagtta
     3361 ttcgttattg gaaattaggc tggttttttt cttctctgat ctttttgttt ctatcatttt
     3421 attggctaat atgggcagga atagcaggtg cattagtaca ggcaagtcta tattactggt
     3481 ttttgtggaa gcaacaagaa aaaggattgg attgggagaa aatggtgcat gtagaagaac
     3541 ataggttgca tcagatctat caatttatca atttattcac agatgttcct gaaattacgg
     3601 caaagatcaa aagaagacgc tatttggatc ctatattgac tcatattaaa agagaatcaa
     3661 aaaacactta tctttacttg tttgcaagaa gattggcaag aggttctgat tttagtggtc
     3721 tttatttccg attagtaatt ataggtgggt tattgattgc cggagtagtc gacttttatt
     3781 ttagtctgat tatcggtttc ttatttttat atttgatcgg tttccaactg ttacctttag
     3841 cgaaccagtt ccgctatatg gttctcgttc agctttatcc aatccataag aatcagaaga
     3901 agcaggcgat tcagtatcta ctcacatggg tattaattat cgcgtcggtt atttttggtt
     3961 tgattgctgc agtcgtatta gatgggcagc agcgatgggt tccgctagcg gcttatttga
     4021 ttgttactat gctctttgtt aaactatatg taccaactcg tttgaagaag atgtcagact
     4081 gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat agagcgttga cagtaagaat
     4141 gctgttcgtt ctattttttt tgagaaaagc tggttttttg aatggcaatt aaatgaaaaa
     4201 gaacgtatat gtgcttgacg tgtagtgttc aggcgactag aataataagt actgcaaaac
     4261 aaagagtaag aggacgggag aatgcatgat ggagtttcag ttagaccaag agtggcggtt
     4321 acgtcccatc aaaggcgaca caggaaaaac atacataggc atgcgtgatg aggacaaagt
     4381 ctttattaaa cgaaacacga ctccgatgtt ggctgctctt tcaagggaag gaattacacc
     4441 aaaacttgtt tggacaaaac gaacggggaa tggcgatact ttgacagcac aagagtggct
     4501 ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga aacgacgtga ttgatgtttt
     4561 gtaccattta catcattcaa attctcttaa aaccatgctg acccgtatcg gtggtcgtgt
     4621 catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa ttaccggaag aactccagaa
     4681 aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag cagatcccgt caatagataa
     4741 ggaaaactta tcagtagtac acggagatgt aaaccatcgt aattggttag tatgtaaaaa
     4801 ttatttatat ctagtcgatt gggattcaat catgtttgca gaccctgcgg tggatattgc
     4861 aacgatttta ggaaattatg tgccactgtc tagctggaat cagtggctga tcagttatgg
     4921 catacgcccg actgacgaaa tgcttgaaaa atttcattgg tatgcggcca tgaatttgct
     4981 ccaagaaatc accagatatt gcaaacgtga agatcatcga cggatgaacg aagaaatttt
     5041 acaattaaaa cgaattttca gtggctgagg tttggcccaa actctgcgga gatttggcca
     5101 gaccttttta attagaggaa aggaatggag ataaataatg cgtgtccgaa atcgtccggg
     5161 agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt agtgatccaa cattgtggaa
     5221 gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc cacattgaga ttggtatggg
     5281 gaaaggccag ttcatcactg gaatggcaaa agcccatcct gaaatcaatt atattggagt
     5341 agaaatgcag gtcagtgttg tttcgattgc actagacaag ttgatcgaac aaccgcttcc
     5401 taacctgaaa cttctacatg tggatggatc agcgttgaca gagtatttcg ctgacagcga
     5461 agtagatcaa atctatctga atttttccga tccatggcca aaaaaacgtc atgaaaaacg
     5521 gcgattgaca tataagactt ttcttgctgt agatgaacag attcttcgtc caaatggaga
     5581 aatccatttc aaaacagata atcaaggatt gtttgagtat tctctggcaa gtttttctca
     5641 atatggcatg atcctcaaac aggtatggtt agatttacat caaagtcaat ttgaaggcaa
     5701 tattatgacg gagtatgaag aaaaattttc ttcaaaaggt caacgtattt atcgagtaga
     5761 agctcaattt caagataaga ccaaaaagaa aagtctgtga aactaacttt ctgctttcat
     5821 aaaaacatta atatccataa ataaatccca gaaaatgaac attacagatg taatgtattt
     5881 tctgggattt atttgattta aaatgtattt atttcatcgg tttttccaca aggtggaatc
     5941 aattggaagc gagaaaatga aaataatcct ttgtcggtga tcttcaattc gggtatgaca
     6001 gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg ttttagctgc tcctagttga
     6061 tgtgctttag ttattaattc attcaactgt tcatagacga tcgaaaaagg ttcctgtgtc
     6121 atgattccgc ctataggaag agaaacacaa gctatttcct gctgtttgtt gacaacggtc
     6181 atcccgccac ctttttgaat caaaagatta gcagcataca gcatatcctc gtcgtttgtc
     6241 ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg tcgctatcgc tccttgtttc
     6301 agctgaaatc ctttcactat gcctaatcca atgtttcccg tttgatggtg acgctcgatc
     6361 accgcaatct ttaattggtc tttttcgatt gaaggaatga atcgttcgcc ttgtcggtcc
     6421 actttttcta ccaaatcttt agtcactaga ctattcggga taatttcgat cacatgggct
     6481 tgtgaagaag aaagaagcag ctctaacgaa tgtggattta atggttgagc gtttagtttt
     6541 ggcagacaac acgtatatga atttgaatga tcagcaaata aatggtcttg gagttgccca
     6601 gattgaacaa ctaatgttcc cttatagaaa acttcatgaa ttggcagctt ggtaagatcg
     6661 tctgttaaga agaaatcagc ttgataccca ggggcaatcg cacctagtgt tttcaaatca
     6721 aaacattctg ctgcattcaa tgttgccatc tgaatggctt gtaatggatc gattccaagt
     6781 gcaatagctt gacggacgat atgatcgatt gatccttctt ttactaaatc attgatcagt
     6841 ttatcatcgg tgacaaagaa gcatcttctt gaattttcag gtgtgatcgc cggtagtagc
     6901 gatgccagat cttttgcaac ggtcccttct cgaaccatta aatacatacc aagatccaaa
     6961 cgttctttag cttcttttgc agttgtagct tcatgatccg tccgaatacc tgctgcagga
     7021 taaacattta actcttccat gccgatacca gctgcatgcc cgtcaatttt tttcttatgc
     7081 tggtgcatca attggatctt ttctaggatg tctgtttcat tatttgctac tgcttgatag
     7141 ttcatgactt cagctaagcc taaaacttcg ggtttttgta aaaaaggggc taattttttt
     7201 gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag gtacacaaga aggtagcata
     7261 aaaaagatat tcatgagtgt ttgtcttgca tcttctatca taaactcaag acctgtactc
     7321 ccagcaatgt ttcctatttc atggggatct gtcactacag tagtaactcc atgaagcaag
     7381 gatacttttg aaaattcgct tggtgataaa agtgagcttt cgatatgcac atggccatcg
     7441 atgaatcctg gcactagata ttgcccatta gcattatgta cgctttttcc ttcgtattca
     7501 ccgattccta cgatcattcc ttcgcagatt gctacatctt tttccatgat ttctttcgta
     7561 aagacgttga cgatcttagc atgcttgatc actaggtcag caggcttttt tttacttgct
     7621 acttcgatat atttccttaa tgttgtttcc aatggagtgc ctcccaagat aaatttttat
     7681 aagtataaca taaattcggt ttttgccaat atttttttgt aaattcattt tatttgaaac
     7741 aaaaagatca aaaagacgta tgttttttgt ggaggggaga ataatggaaa taaaacgatt
     7801 atcggatcaa atgataagtg aaagttttgc caaacatgcc caagacgtat atatattgcc
     7861 agcttcagaa gtattttaca tctattttcg ctatggaaaa aaacgaacat tctggaagaa
     7921 agtctcttcg gcagatggac agcaactgat ctcaagattt aagtatttgg gacagatgga
     7981 tatcggagaa aaaagaaaag ctcagttagg tgcatttact tattctttag aaaaacagga
     8041 agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa cgagagagtt tagtgattcg
     8101 gctattatac ggtacttttg aaacacccca tcgttgcttt agttctcgag attttgcaac
     8161 tattttacag aaaaccgtca gacgtggatt ataccttttc agcggaccag tcggatcagg
     8221 caagacttct ctaatgtatc ggttggcttt ggaagaaaaa ttgcaagtga ttactatcga
     8281 agatcctgta gagatagaag aaccgttttt tctgcagcta cagatcaatc cgaaaatagc
     8341 acagacttat gatacacttt tgagactcgc attacggcat cgtccagact tgctaatcat
     8401 tggcgagata agagatgaat caactgcaca tgctgcggta agagcagcac taacagggca
     8461 ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt acattggtca gaatccatga
     8521 actgacaaag aaaaaagaag agctatctga atgtttagct ggtgtcattt atcaagaact
     8581 tttggaagat gccaatcacc atccttctgt tttatggagt tatcaattta tggaggaaga
     8641 atcagtaaaa aaatcatggg aggaaagtta tgaagaagct caggaaaata tttggcagtc
     8701 agaactattc taaaggatta acaagaagcc agcaaaacca gtttgcacaa accatgggag
     8761 aactgctttt atctggtttt agtctgcaag aatcccttta tttattactc acgatccgtt
     8821 cgtttccacc tgttattttg gcaaatatcc aacatcagct aaaggaaggg caagcgttta
     8881 gtgaggtact ttcgcaaata ggatatggag cagagcaact ggtacagatc gagttagctg
     8941 aacgtcatgg gaatctgccg acaactttgt tggaaattgc caaacagttt cggcttgttg
     9001 aaggatttcg caatgatttg aaaaagctgc ttgcatatcc gatgctattg ctcacctttt
     9061 tgctaggtat cttgtttagt atgcggttgt tcgttttgcc tcagcttatt ggttcaggaa
     9121 tggtcatgca agagcattgg ggtattcagc tgatacaagt ctttcattgg tatgttctac
     9181 tattcatcct agcggttact ctattggctt tactagtaag gtatcgtctg atgaagttgt
     9241 ctcctgttga tcagagtgaa tggataagca gacaattatt gattggaccg atatattcct
     9301 tgtatcaatc ggcttatttg tcactagaga ttggaaagct ttttcatgaa ggtttcgaat
     9361 taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga aagtctgatt cagcatcttg
     9421 cctctaagtt gattcaagga ttggaggcag gtatgtcatt ggctgagcag ttccagaatt
     9481 atcgttttct atcagaagaa ttcagtaaaa ttgttttaca gggagaggca aagggaagtt
     9541 taggtaaaga actgattttt tacagcgaat ggacaagaaa acagcttttc aaacgtcttc
     9601 atcactggat gcattttatt cagccaatca tttttttggg cgtagcggct ttgattttat
     9661 tagtttatgc cgctgtttta ttacctgttt acggaagcat agaggaggtt ttgccatgaa
     9721 aaagagaaaa atatcttatt atagcggttt tacattgatc gaaatgctaa tcgtcttact
     9781 gatcatcagt gtattagtgt tgttgttcgt ccctaatttg tcgcgttata ggaatcatgt
     9841 agaccaagaa agtcgtgaag cgatcatcca gttggtagac acacaaaaag agctctatgc
     9901 gcttcaaaat aatggaagag ttcctactgt ggaggaattg ttgaatgaag gctatatcaa
     9961 acgagaacat gcagaaatct atcagcggcc ttgaaggtaa ggttttagca tcaggatata
    10021 cgttgtttga atcactctta ctgattttat tacttacaac gatcctttct tttccagttc
    10081 tcgcattttc tgcatggaag aatgaattgg cagtctatca atttttcggc caatttgaaa
    10141 aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca gatccaaaca ggcttttatt
    10201 ggaatagtga agagaatcaa atcatttttc atgttccgaa tccagataaa ataaattggg
    10261 aagtattaga tattcctgat gagattacct tgaaacgcca tgcttctatt acttttgctg
    10321 ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt ttattgggaa gaaaaagagc
    10381 ggacgattac gtatcaattc cagataggga gtggtcgcta tacaaaaaga attgaataaa
    10441 acaggatttg ttttgttgga gtcccttaca gcgcttatga tcgcttctgt ctgtgtctac
    10501 ttagtgagct ttggccagct gtttttgctt caggcagaaa agaaaagtgg ggaggaactg
    10561 ctatttattc gaatgattta tgaagatatc aagacgcagc ggatgtatca caaaagacca
    10621 gagcgaaggg aagaaatgat cccatacgat atccaaattg tcgataataa tggagtaatc
    10681 gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta ttgaaaaaac aagtaattcc
    10741 agcatttacg ctgattgaat gtctaattgg actgatgata ttgagcagtc tattgctcac
    10801 attcaatctt ttgattcagc aaaccatcca agtaagtgaa tttttgcagc gtaaagatca
    10861 aaaagagtgg ctaatatttt tagcacaatt agaagaagaa ctcaagagca gtcacaatgt
    10921 ctctgtgaaa aatcagcaac tgcactacac gatcgaggat aaacagtata tcatcgaaag
    10981 atatcaggca atgatacgta aaagaagaac ttccggcgga catcaaccta tgttaacaag
    11041 tatcactgag cttcaattat tggaaaaaga acacacaatt tctttttatg tacactttga
    11101 aaatggagaa gatggatatg ccatatggac aaaaaacgat cagtagaaaa tgttataagg
    11161 gctctatttt attttctgtc ttgcttttat ttcttttatg tacgttttta ttgatggcag
    11221 cattagaaag ctaccgtttg tcaatggatc ttaatatacg aatcaaaaat tattatatag
    11281 caaaaataat gaagcagatg gctttatcgg aacagtctac gcttgccgaa agttcggaag
    11341 gagtttttta ctacacaact ggatctgtaa catatcagtg ggtacaacag tccctattct
    11401 tagaggtgga agtctcaccg tttatctttc ggttcacaga agaagtaaaa aatgatacag
    11461 atactagtac agaatgatgg aatgttcgtg aaaacgccct cttttctgtt ataacactgt
    11521 ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac agtgaaaacg attgaaattt
    11581 tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt tataatatat ctagaaatga
    11641 ggtgcaccat gttaccagaa aaaatggaac aagggtttaa tcaaaacctg gaagctattc
    11701 agcttttaca gaatgcattg ggaacttcct ttttagaagc ctatgtcgaa aatgcagaaa
    11761 atttaataga tgattatcaa gtacgtgtgg tggatggagt gccgacaaaa gagacaacgc
    11821 aacggatcac agctctttat gaagagttga agaaactttc tttcgagcca gaagaatggc
    11881 gcagactgtc tcaattactt ttattgaagg gcagccagac agaacatttg caggcgaacc
    11941 atcaactcac tcctgatagt atcggttttt tatttgtttt tcttattgaa caactataca
    12001 caaataaaaa agcaccggtt aagattctgg atattgcggc aggaatgggc aacttgttat
    12061 taacagttct tttaaaccta tcgaatgcgg ggtaccaaac agaaggaatt ggtgtggata
    12121 ttgatgatac attgttggct gtcgcagctt ctacaagtga tttgacacaa gcaaatgttc
    12181 aatactttca tcaagacggg ttgcaggaat tattaataga tcctgtcgat tttgcaatca
    12241 gcgatttgcc aatcggttat tatccgaatg acgaaaaagc aaaagagttt ttaacaagta
    12301 ccgaagaagg ccatagttat gcacatcatt tattgctaga gcaatcaatg aaatatgtga
    12361 agccggatgg ttttggtttg tttttgatgc cgagtggatt tttagaaacg gatcaaagtg
    12421 aagaaatcaa aaaatggttt aaagaagaag gttatcttca aggaatgatt caattgccag
    12481 atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt gatcttacag aaaaaaggac
    12541 cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact tgcttcatta aaagaaccag
    12601 agaaggtgac ggaatttttc aacgagttta aaaactggaa atcatctgct ctgtaatcat
    12661 tcaacgagaa ggagagtcat atgtctaaaa caattgcaat caatgctggg agttcaagtt
    12721 tgaaatggca attatatcaa atgccagaag aaaccgtagt tgcgaaaggg atcgtcgaac
    12781 gtatcggttt acaagattct atttttacca tcaagtacgg cgataatcaa aaattcgaac
    12841 aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat gctgttagat cagttgattg
    12901 aactgcaaat tttaagctct tatgatgaaa tcaccggagt agggcaccgt gttgttcacg
    12961 gaggagaaac ttatggggat tcagtagtga tcgatgagaa agtaatggac cgcattgctg
    13021 aattagcaga atttgctcct ttgcataatc ctgcaaactt aatggggatc aaagcgttta
    13081 aaaagatttt acctgacatt ttgagtgtgg ctgtattcga tacatctttc cactcaacaa
    13141 tgccaaagca aaattattta tatagcttac cggtgtctta ttatgaagat ttcaaagtaa
    13201 gaaaatatgg cttccatggc acaagtcatc gttatgtgtc ggaacgtgcg gcaaaattat
    13261 tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca cttaggaaat ggtgtatcga
    13321 tcacagcagt cgatggaggt aaatccatgg atacttctat gggattcaca ccattagctg
    13381 gggtgacaat gggtacacgt tcaggtgata tcgatccagc tgttctccca tatttaatgg
    13441 aaaaattaga gattgatatt gatgaaatga tcaatgtttt gaacaagaaa tctggtctgc
    13501 taggcctaac aggattgtct agtgatatgc gtgatcttga aaagaactat gaaaaagaac
    13561 atattcgttt agcttatgac attttcgtgg accgcatccg aaaatacatc ggcggttatg
    13621 tgacaacgat gaatggtgtt gatgcaattg tctttactgc tggtatcggc gagaatgacg
    13681 gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt tggttgtgaa atcgatccag
    13741 cgttgaatga attgcgtggt gaagaagtag atatctctac aaaagaatca aaagtaagag
    13801 tattagtgat cccaacagat gaggaactaa tgattgcccg tgatgtggag cgtctgcgcg
    13861 gctaagccaa agcattcgtt aggataaaag atgaaacgca tgcaagaggg aattcttgca
    13921 tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta acttcaattt cccttctttt
    13981 ttacaggatc ttcttaggga attttgcttg gaaaagtggt caaaagggaa aagactggca
    14041 ttttatttat tgattttcag gaaaatccca atattttatt ttcattcatc tttataaggg
    14101 acattacata tgtaaaaaga aaaaacattt ttagatcagc ctcgtgggct gtcgacttta
    14161 ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca ttcattgatc gaataataaa
    14221 aacttgattt tccacctgta ctttgcctga atcgtgaaag cacaggtatg aaaatcaagt
    14281 tttttgttgc gaaaaaacgt cctagtcaat cagttggccg agcggctggc ggctgatctg
    14341 ctcctgatca ataacgagtg cttcttggaa gtgttgctgc aaaaagtagc ggtcatggct
    14401 gactgctagg agcgttcctt gatagtcttg cagcatttct tcaatttctt ctcgagcata
    14461 gctgtcgagg tgattggttg gttcatccaa aatcaggacg ttgatttttt ctaaaaagag
    14521 tttcagcaac gccaaacgga ttttttcacc accggataaa tctttgatcc gtttacccac
    14581 atcgctttga taaaagccac ttttggctaa ttgccgtcga gcgacttctt cgtttccagt
    14641 caatgtgatg gcgtaagcca gcaaacggct atcgggatcg gcaaactgca gctgctgcgg
    14701 caaataaccg atttgcaagc tactacctaa tttgatggtg ccaccgtaag gcttttcctc
    14761 accgagtaat aatttgagta aggttgattt tcctgcacca ttgtcgccga tgatggctaa
    14821 atgatctcca cgatagactg caaaactgct gtctttgaat agtacttgat cagcgtactg
    14881 ctggtgcaag ttttgaatga tgaaaacttc tttgccagat tttccaccat tagacaagct
    14941 ttgcagccga tttttcgggg gaggtggcgg tttgacgagt gtcagtttcg ccaggcggcg
    15001 ttccagttct ttggcttttt tgaagaagga ctcattgtct ccttcatgtg cccactgacg
    15061 aaaacgacga atcatgactt tcaaccgctg gacttctttt tgctgcagtt cgtaattttt
    15121 tgtcagctca gccaagcgag cttctttcaa gactcgaaaa cggctgtagt ttccgggata
    15181 ctcaatcaat gcaccttctt ccagttccca tgtttttgtg gtgacttgat cgagaaattg
    15241 ccgatcatga gaaacgacca gaaccgattt ttttgtattc tgtaaatagt tttccaacca
    15301 gcgaatactg ttaagatcta aatgattggt cggttcatcg agtaaaagta aatcggcatc
    15361 ggctgctaaa attttggcta aagcgacttt aactcgttcg cctccgctaa gttctgttaa
    15421 ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag atcgctgcaa tccggtcttc
    15481 taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc tcaccgtaac gggccagaca
    15541 cttctctaag tttgtatctg gttctgtcat caatgcttcg tagcgactca tttgctgttt
    15601 gaggtcctgt atctcagcgg cagaatggag caagtagtgg ttgacggtcg cttcggaaac
    15661 tgtaagttct tgttcgaccg tcccgatctt taagcctttt ttgcgactga taacaccgct
    15721 gtcgacacct tctctgccgg tcaagatttg taaaaaggtt gatttgccag ttccgttgat
    15781 accgactaag gcaatttttt caccagcttc aatttgacag gataaatcct caaataatgg
    15841 attcccgccg attgtttttt taatagaaat tgcttgtatg atcatgatct ctctccttga
    15901 gaccactcaa aaaggccatg gttatccatg gcctgtgtta tttaaactca gattatcatg
    15961 agaaaaatac cttgagatgg ccattgattt tacgtgaatt caactgtttt ggacaaactt
    16021 gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa atgcgatatg gtatcgttgg
    16081 taattttcat tacttgttat tttagtcatg cgcatcatag tattttccct ccttcgtgta
    16141 gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca actagcgctg acatttactt
    16201 aatatttatc gatgtgtaat tctgtcagag agcttttttt atctgactga gggtaatgtc
    16261 tcgtggacca catcattgta ttctgagcta aaccagcgtt caggcgaaat catatggtgt
    16321 ttgataaaaa actttgtcta catgacttcc ttttgccatt tgcttaatac cgttgcatta
    16381 aacagctctt ctctttggaa atctcaccaa catcgcgttc aggagtatcg ttgtggttgc
    16441 aatggcaagc ggtagccttt catcaattct tgaatgattt accgaatgta gcacttgcct
    16501 tctcgcggta cccgaacccg aaataaaatt ttagtttggc gataaaatcg gtatccgcca
    16561 tcgatacttc atattttttc gtatcagatt catcggaaac ttccacattg acgaaccaat
    16621 aagctttggc atgcttaggt cgttttaaac cacaccaggg cgactaatcg ccctggtgtt
    16681 cacttttttt tattttactt gaactcattg actgttttcc ctttatttta aacattataa
    16741 agaccgatga gtgcttgccg cgtcttttgt ttttatcacg gttttttgtg caaaaaaacc
    16801 tgccgatagt tggcaggtcg tctacgtgtt aagctttggt gtggtcttta gctggcaatt
    16861 ggttttctaa atcagttcgg acaacaagca catcgcaagc tgcgtttcta ataacatatt
    16921 cagaaacaga accaataaat aaacgttcta cagcattcaa tcctgtggct cctaacataa
    16981 tcaaatctac ttgattatct tctgggattt ctctggcaat aatttgtttt ggtgagccat
    17041 attctacgac agaggttaca ttattcaagc cagctttctt cgcattgctt tcataatctg
    17101 ccaatgtttg ctttgccatt tcagtagctt gttctgcaag cattccatca aaggatgaaa
    17161 cggtttggaa agaacgtgtg tcaatgacat gtgctaatag aagttctccg ttatttcgga
    17221 tagctacatt gaccgccttt ttaaaagcta attcagcttc atcagaacca tctacggcaa
    17281 ccatgatttt tttgtattgt tgtaacataa gcattcactc ctcaagtcaa tcgtattgta
    17341 ctcttttatt gtagaacact ttagggaaaa agagaattat ttactgcttt ggttcagcaa
    17401 ttttttttgc aaatcggata ctgaaggtgg cagaaacaat aattacgatc agtaaaaaga
    17461 ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat acccattacg cctaaaacag
    17521 catatgcagc tccgtaaaat tgtaggaatc cttgattttt atcatttttg ttgatcaata
    17581 caaaaaagat atcctgttta ttaaataaat aatagctaac gaataataat actaaagtgg
    17641 cgataatcat taaaatccta atcatgaaca aatcctccaa aataagcaac aaatagaaaa
    17701 agcaatttgc caagagcaaa ttgctttatt ctttaaaaga tggtaatctc cgttaatgcc
    17761 gttgtttgtc ctgtagtatt gatcccgcga attcaagcag gtgggtccca tggtcgtagc
    17821 ttcgaactac caaatgataa ggcatgttac cagcagtgac acaagacggg tcccaagata
    17881 tcaataaaaa tgttcggtca acacacaaag aggacaattc gaacatcaca gatttcccac
    17941 ctctatagta gcatatgaat ttaaaaagag caatgagaga gctcgcagaa acagaaccgt
    18001 tttcttgtct agttttttgg aggatgcttt ttccactctt ttatacggta atactgttga
    18061 cccagagcct gttcatattt ccctgtatct tttggctgat aatactgagc attcttcagt
    18121 ttatctggta aatattgctg attgacccaa gcctgatcaa aatgatgagg atactgataa
    18181 cccacgccac ggttgagtga tttcgcacct ttgtaatggc tatctctcaa atgatcagga
    18241 acatctccag ctttgccctc tcgaatatca gcaagtgctg catctaatgc catgtaggca
    18301 gaattggatt ttggcgacaa gcaaagatcg acgacgacgt ctgctagtgg gatacgtgcc
    18361 tcaggcaatc ccaatttttc agcagctaat acagcgttca ctgtacgagc cgcagcagca
    18421 ggattaccta aacctatatc ttcataaccg ataaccatca gacgccggca aatacttgcc
    18481 agatcacctg cctcaactaa acgggcgaga taatgaagtg cagcatccac atcacttcca
    18541 cgaatcgatt tttgaaaagc cgaaataacg tcataatgag catctccatt tttgtcatgg
    18601 gtaagagctt ttctttggac acattcttca atgatcgaca gtgtaagatg aatccttcct
    18661 tctttatctg aaggagtcga tagtgtagcc agttccagcc cattcaatgc actccgcaaa
    18721 tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt cgtctaactg gattgcttgt
    18781 tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga ctgccaactg gatgtcttgt
    18841 tctgttaatg gtttgacttc aaagatctgt gtacggctgc ggatcgctgg attgatggta
    18901 atataaggat tctcagtagt cgctccgatc aagatgattc ggccactttc taaatgagga
    18961 agcagaaaat cctgttttgt tttatctaat cggtgtactt catccaataa gaggatcact
    19021 gtaccactca tttttgcttc ttccgctaca acttgtagat ccttttttga atctgtagcg
    19081 gcgtttagca tgcgaaatgc ataattagta gagccggcga tcgcacttgc gatactcgtt
    19141 tttccagtac caggaggtcc atatagaatc atggaagaga gcatgcgagc atcgaccatg
    19201 cggcgtatga tttttccttc cccgactaga tgctgctgac caacgacttc atcgatcgtt
    19261 cgaggtctca tgcgataagc caatggctgt tgcataggat tttcctcctt cttgttttta
    19321 cgtcccttaa ttatatcata tttagcaaga tgcctagaaa gaacagcctt ctgttgtttt
    19381 gttttcatag cttatccaac taatattgtg tatgataaag taggtgagaa aaatgacgga
    19441 taaacaaaag atagatgaat ttttttggaa taaaacgacg ccagaagtgg cacgggattt
    19501 attggggatg tatttggagc atgacacacc agaaggaaga ttagctggat atattgtaga
    19561 cgcggaagca tacctaggtc cagaagacga ggccgcccat agctatggtt taagaagaac
    19621 accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc tacctttaca cgatgcatac
    19681 tcatcggatt ttgaacatca ttactcagcc agaagggatt cctcaaggag taatgatacg
    19741 tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa aatcgtggcg gaaaaactgg
    19801 tccagatatc agcaacggtc cgggaaaatt agtggaagca ttagcaattc ctcaagaact
    19861 ttatggtcag tctatagcag atagttcgtt acgcttagtt tttgagaaaa aaaagacacc
    19921 aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac aaaggtgtat ggacagaaaa
    19981 gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct ttacaaagaa aaaaccaaat
    20041 cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa ggatctatta acatatttag
    20101 atgaaataat tgaaaaacaa gtgacagact atgatgtagc tttagattgg gatacaaaaa
    20161 atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa taaagcacaa tttgagattg
    20221 atgatgcaga agggatcgtt tcgcaagaac caattatcga atttgaagac ggcgtacttc
    20281 tttttaatcc tcagaaatct gtttttgatg aacgcaatta tctagcagta atcccttatg
    20341 aaggaaaaaa agggttggca aaatcagtag ctgattcttt agtggaatat ctaaatgaag
    20401 tattagctca agggcaaagc gatttgcttg attttttgga tgaagacgat gacgaagctg
    20461 tattcgagct tcactgggat aatcagcaac tagcagaatt agtagaagta aaacagcaaa
    20521 atgaagcaga tacttattta ccgtacccaa gttattaagg agtgacagat atgaaatgga
    20581 atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc agttgctaat attttgatgg
    20641 aggcaggggc gagtggtgtc gcgattgaag attctttaga tgttgaacat tttcagtcgg
    20701 atcaatatgg tgagatttta gataaaaatg attttacatc gctgaaagaa ggcgcactag
    20761 tcatggctta ttttccggaa acgactttct taccggaaat cttgcctttt atcaaagatc
    20821 gtatcacaca attacctgag tttggtctag ctattggaaa aaataaagta acagttagtg
    20881 aagtagagga aagcaactgg gcaacagcct ggaagaaata ctatcatccg gtgcgtatta
    20941 cgcgaatgct gacggttgcg ccaagctggg aaaactacca gacagaagat cctctagaaa
    21001 aaattatcac actagatcct ggtatggcat ttggcacagg cactcatccc acgacttcct
    21061 tgacattgac agcactagaa acagtcttgc gtggtggtga aacattgcta gatgtgggaa
    21121 ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg tgcaaaagat gtctatgcct
    21181 ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa tatggatatg aatgaaatag
    21241 ccaaagatgt ccatgtatca gctaacgatc ttttgacagg aatcgagatt gaaagtgatg
    21301 taatcgttgc gaacatttta gcagatatca tcttattgat gatcccagat gcttggcgtt
    21361 tactgaagca aacaggcacg ctgatcgttt caggaatcat tgaagagaag aagcaaatgg
    21421 ttctggatgc catgttagaa caagggttta tcgtggatca aattttccag caaaaagact
    21481 ggtatgcaat tattttgaaa aaaccagagg tggaatagat gcagcggtat tttttaaaag
    21541 aatcatatga atcaaaagac tattttgttt tagaagggga aaactatcac cacattgtac
    21601 gggttatgag gatggaaccc gaggatgaag tttttttggc attcaatgat caaatagcca
    21661 ttgttgcaga aatcacggag attactgaag aggctgttta tctgaaagaa attaaaaaag
    21721 aagaaaatca aaaggaactg ccaagttcga ttaccatagc gagtggctat cctaaagggg
    21781 aaaagctgga ttggatcgtt cagaaatcca ctgaattagg agcgcaagct tttcttggtt
    21841 tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa acgtatgaaa cgacaacaac
    21901 ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca tcggcagatc gtgccatcag
    21961 tagagttatt ggaaacgaaa caagatttat ttgagcggct agatgattat gacgtggtct
    22021 tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc aaatgcagcc aaaattctta
    22081 gtgaaacaaa accagatagt cgaatcttag ctgtctttgg accagaaggt ggattatccc
    22141 gcgatgaagt cgattatttt gtagaacaca aagggatact ttgtggatta ggaccaagaa
    22201 tccttcgtac agaaacagca cctctttatt tattaagtgc tgctagctat caatgggaat
    22261 taatgaatcc ttctttcagc taacgttctt cattaataaa agagtatcga cagaaagaat
    22321 tgaaaaaata gtcaacaatt tgtataatag aaacaattat ttatacaatg gatacgaaaa
    22381 atcgtttgaa aaagctttta aaaagcgaac atttccgtaa aaaaacgtat aactgcacgg
    22441 attttgggac aattatcaaa cagatatttg tcccgattcc cgttttaccg aaaaaagaaa
    22501 gggggatcga ttcctgtgac aaaagaagaa attttaacgg gacccggtgt cataaagctc
    22561 gttagtcaat acatgggtcc cgaacatgta gcatttgttg agaaagcatg cgaatacgcg
    22621 actgctgcac atgacggaca gtttagaaaa tcaggcgaac cctacattat tcatcctatc
    22681 caagtcgcag gtatattagc agatttaaaa atggatcccc atacagtggc tacaggcttc
    22741 ttacatgatg ttgttgaaga tacagaaatc actttagaag atctgagaga agaatttggc
    22801 gatgacgttg ctatgttagt ggacggcgta accaaattag ggaaaataaa atataaatcc
    22861 cacgaagagc agctagcaga aaaccaccga aagatgctgc tagcaatggc tcaagattta
    22921 cgagttatca tggtcaaatt agctgataga ctacataaca tgcgtacgtt gaagcacctg
    22981 cgagaagata agcaaagaag gatcgctcag gaaactttag aaatctatgc gcctcttgca
    23041 catcgtctag ggatcagccg gatcaaatgg gaattagaag atacagcact tcgttatcta
    23101 aatccaaaac agtattaccg tatcgtccac ttgatgcaga cgaagagaga agaacgtgaa
    23161 aaatacgtaa gtggtactgt tgaagatatt cgaatagcga cagaagagct ggggattttt
    23221 gcagaaatct atggacggcc aaaacacatt tattcaatct atcgtaaaat gaaagatcag
    23281 aaaaagcagt tcaacgaaat ttatgacttg ctagctattc gagtgatcgt agattcaatc
    23341 aaggattgtt atgctgtatt aggagcaatc cacacaaaat ggaaaccaat gcccggcaga
    23401 tttaaggatt atattgccat gccaaaagct aatatgtacc aatctttgca tactaccgtg
    23461 atcggtccgg caggaaatcc ggtagaaatt caaatccgaa cacaggaaat gcatgaaatc
    23521 gctgaattcg gggttgctgc acactgggcc tataaagaag gaaaaaatga aaaagtagaa
    23581 ccagatggta tgacgaaaca attaagctgg ttccatgaga tactcgaact tcaagacgaa
    23641 agctatgatg cttctgaatt tatggaaggc gtaaaaggag atatctttag tgataaagtc
    23701 tacgtcttca caccaaaagg agacgttact gagttaccaa aaggatccgg accattggac
    23761 tttgcataca gtatccatac agatatcggt aacaaaacca ctggtgcaaa agtaaatggc
    23821 aaaatggtgc agcttgatta caaattgaaa aacggagata tcattgagat catgacttct
    23881 ccaaattcat ttggcccaag tcgcgactgg ttgaaattag ttgctactag caaagcaaga
    23941 aataagatca aacgtttctt caaagcccaa gatcgagaag aaaatgtgat caaaggccac
    24001 gaatccgtgg tcaaatgtat tacagatcta ggatttacgc ctaaagatat tttgacgaag
    24061 aacaaactgc aagaagcact cgatcgtttt aattatcaaa cagaagatga tctctatgca
    24121 gctgtagggt atggagaagt tagccccttg acgatggcca atcgtctgac tgaaaaagaa
    24181 cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag aagaaatcat gaatcagccg
    24241 aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg gcgttgtcat tcaaggggta
    24301 gaaaacttac taattcgtat cagtcgctgc tgtaatccga ttcctggtga tgatatcgtt
    24361 ggttatatca ctaaaggcag agggatatcc attcatcgtc gagattgtcc gaatgttcag
    24421 cctgacaaac caaatgtagc agaacgtttg attgaagtcg aatgggaaga tacatcgaat
    24481 acacgaaaag agtatgatgc agatttggaa atttacggct ataatcgttc aggcttattg
    24541 aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc tcaacagcgt tgaagcacgg
    24601 acgaataaag ataaaatggc gacgatccat ttgacggttg gtatccagaa tttatcccac
    24661 ctaaagagta tcgtggataa aatcaaagca gtacctgatg tctacagtgt acgccggacg
    24721 aatggatagg aggacagata tgagagcagt tgttcaacgg gtaagtaaag ctgctgtgtc
    24781 agtcgatcag caagaaattg gaaaaattgg ctacggccta cttgttttat taggcattca
    24841 tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa aaaattgcac aaatgcgtat
    24901 ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa gatgtgggag gacaaatttt
    24961 aagtatctct caattcacac tgtttgcgga tacgaaaaaa ggaaatcgac caagctttgt
    25021 ggaagccgca cgaccagaaa cagctattcc tttatacgaa tcattcaatg aaggaatcag
    25081 aagtcgcggc atcacagtcg ctaccggaca attcggagca gatatgtcgg tttctctcgt
    25141 caatgatgga cctgtaacga tcattattga ttctaaaaac aaataaagta atctggtttt
    25201 cactagtaaa aactatgaaa cacgtcgctt aagttagact gataaactct aacttaagtg
    25261 gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc ggaaaaaaag ttaagcttaa
    25321 attatacaag ttatttttta taggaggttg ctgaagatga aagaagtacc agtagtagat
    25381 atcaaaatca cagatttaaa aaaattgtat caaaaaggat acaacatgtt ggaggagtta
    25441 cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt tcaataaaga agctatcacg
    25501 atctacggat catcagcagc aaaagtattt tatgatccaa ggaatttcaa acgaaaaggt
    25561 gctatgccaa aattagtatt gaagacactt tttggtcaag gtggagtaca gactttggat
    25621 ggagctgctc atcaccatcg gaaaaatatt tttatggatc taatgacacc ggaaagaatg
    25681 gaagattatc atcgtatctt agataagaat ctgacacaag cacttgaagc acagcatggt
    25741 caatttgaac tatttgattt aagtaagatg gttttcttta catctatttg cgagtgggca
    25801 ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa aattagcaga atatcaaatc
    25861 tctatgatca gcggaacgtt tacttctccg attgatcaca taaaaggtgt cgaaaatcgc
    25921 aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag ctcgacaaaa tcctgtggct
    25981 ggaaaagaaa atgtggcatt atatgctttt gccaatgcca cagatttaga tggtcagctt
    26041 ttacctctgg aagtggcagc tgttgaatta ttgaacatca tccgtccaac ggttgctcta
    26101 actgtgtggg cggcattgat gggacatgca cttttcagta gaccagattt gtatcagcaa
    26161 ttaaaaaatg acttttcgac gttacaagat ccgtttattc aagaaatgag acgctattat
    26221 cctttcttcc caatgctacc tgcgatttcg ctgaaagaag tagaagtaga tggttatcgt
    26281 ataccagaag gcagttgggt tattttagat ttatatggaa cagaccatga tgaacgaaca
    26341 gttgaagcgc cagattcctt tatgatcaaa cggtatgtag ggaaagcaaa agatatttcc
    26401 tataaagaag aatatgagat gattgcacaa ggcgggggaa attttcgaca aatgcatcgc
    26461 tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt tttccgatca gctagtaaat
    26521 aagtttgagt tctctgtacc tgaacaagat tggacgattc cattcaatca gtttccgact
    26581 tatccgaata gccgagcgct actttacaag aattagatta gcataaactt atcgaaaaaa
    26641 aggaagatca aacagtgctg atcttccttt tttcatatta ctataaattg ataaggtcaa
    26701 ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata atcttcattg gataaatgca
    26761 aatctaaagc ttggacattg tcagatactt gctctggacg cttagctcca ggtataacta
    26821 cagacactcg tggattcttc atgtaccaag ctaaaacaag ttgcgcagga gtggcttggt
    26881 atcttttagc aatggggcga agtacatcga cagcggttaa aatttctctg aaacgttctc
    26941 cttggaaatc tggatttctt tttcgaggat ctccttcacc aaattgtttt tcctcaccaa
    27001 gttcatattt tcctgtcagc aaaccggaag caagaggaaa atagggaaca aaactgattt
    27061 tgtttttttc aagataaggg aataattctt tttcagcctg ccgatggatc aaactgtatt
    27121 tatcttctac tacatcgaca tatccatctg cattcgcttc tttcaattgt tctaacgtga
    27181 agtttgacac accgactgct cggatttttc ctgcttcttt taattcgtgc aatgtagcaa
    27241 ctgattcatt ttttggtgtg ctttcatccg gaaagtggat ataaaaaatg tcgatgtaat
    27301 cagtttgcag acgttttagt gcatcttcca ccgattgttt gagaaactca ggagaattat
    27361 caaatgttcg ccctttattt ggtacatgag cagcttttgt agcgatgacg acacgtgagc
    27421 gatcatattc tttttccttc agtacctcgc caatcagttc ttcggaacgg ccgtttccgt
    27481 aagcataagc ggtatcgatc aaattgatcc cgctattcaa agctgtacga actacttgtt
    27541 ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc tccaacggca tttgctccta
    27601 atccaagctg ttctgcgtag acttgtgaat gtccgattct tatttctgcc atagctgatt
    27661 cctccttttt gtctagtcta aaacaaaata ccaaaaagct gaactaaaac gtttttgtga
    27721 ggggaaatag tcagatatct cttgactttg agactggttt ttagtaggtt ataagtaata
    27781 tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt acaagagagg atcatcgagg
    27841 ctgaaaatga tctaaagttt cattagccta aagacactct ggagatggac gagtgaaaag
    27901 ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa atctttcaaa attaggtggt
    27961 accgtgtatg aatttcattc acccttgttc atgatgaaca agggtgattt ttttatttac
    28021 atagctaaga aagggagaga aaactatgag tttccaaaga ccaaaaggaa caaatgatat
    28081 tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa acagcacgtt tattattcag
    28141 agattatcag tataatgaaa tgcgtacacc gatttttgaa cactatgaag tgatatcccg
    28201 aagtgtaggg gatacaacgg atatcgtttc aaaagagatg tatgattttt atgacaaagg
    28261 ggaacgccat gtgacgcttc gaccagaagg tacagcacca atcgttcgtt cttttgttga
    28321 gcataaattg ttcggcccag agtatgcgaa accgttcaaa actttttata tgggaccaat
    28381 gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa ttccatcaaa tcggagtaga
    28441 agcatttgga tcagaaaacc ctgcaactga tgtggagagc atggtgatgg cacttgattt
    28501 cttcaaacag ttggggatca cgcaaatccg gctagtcatc aattcattag gtgataaaga
    28561 aacgcgtcaa gcctatcgtc aagcattgat cgactatctt ttaccttttg aatcagagct
    28621 gagtgaagac tctaaacgcc gccttcatga aaatccatta cgcgtattgg acagtaaaga
    28681 caaacgagat caaaaatttg tggcagaagc tccatctatc ttagattatc taagcgagcc
    28741 tgctcaaaaa cattttgata ccgttgtttc catgctggat gcattggctg ttccttatga
    28801 aatcgacagt aatatggttc gcggactaga ttattacaca catacgattt ttgaaatcat
    28861 gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc gcaggcggac gttatgacaa
    28921 tctagtggaa gaactaggtg gtccttcaac acctggcttc ggatttgcaa tgggtatcga
    28981 gcgattactc ttgactatgg aggcagagga agtagttatc cctgcattta atgagttgga
    29041 tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa gcattgaaag tggtccaagc
    29101 gattcgaaac tttggttttt cagctgaccg cgacttcatg aaccgtaaag caaaagcgca
    29161 atttaagaca gcagataaag cgaatgccaa attagtattg gtgatcggtg aagatgagct
    29221 tgctagtcaa acgattaatg tcaaagcaat ggcaagccga aaagaaaaaa gttttccatt
    29281 agaagagata tatgaacatt ttgatgaagt ctatgatgaa atgactttat tcgagtagga
    29341 ggaaaaaaat aaatggcaca aagaacgatc tattgcggat tagtttccga acaacaatta
    29401 gaacaaacag ttacattaaa aggatgggta caaaaaagac gagaccttgg gggagtaatc
    29461 tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg tctttaatcc tgaaatcaac
    29521 aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat acgtgatcga agtgactggg
    29581 gaagttagaa gacgcggaga aaatgcagta aatcctaaaa tggccactgg tgaattcgaa
    29641 gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa ccccgccatt tcaaattgaa
    29701 gatgatcaag caatcagtga cgaaatacgt ttgaaatatc gttatttgga tctgcgtcgt
    29761 ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa caaaagcgat tcgtcattat
    29821 ttagatgata atgactttct cgatatcgaa acaccttacc tagggaaatc tacaccagaa
    29881 ggcgcaagag actacctagt tccttcacgt gtccatgctg gacatttcta tgcgttgccg
    29941 caatctcctc aattgtttaa acaattgctg atgaatgctg gattcgaccg ttattatcaa
    30001 atcgttcgtt gtttccgcga tgaagattta cgtggagacc gtcagcctga atttacgcaa
    30061 gtagatattg aaacaacgtt cttaacagca gaagaaatcc aaacttacac agagggattg
    30121 attgcgaagg taatgaaaga agttcgcggt atcgaagtca cattgccatt tcctcgtatg
    30181 acctatgatg aagcaatggc tcgctacggc agcgacaaac cggatacacg ttttgatatg
    30241 gaattgatcg atctaagcga tacagtcaaa gaagtagaat tcaaagtctt ccaaatggct
    30301 cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag gcgctgcaga tcgctactca
    30361 cgaaaagaca tggatcagtt aggtcaatat gtaggacaat tcggagctaa ggggcttgca
    30421 tggctgaaag tagaagaaga cggattgaaa ggtccaattg ctaaattcat gggagaagca
    30481 acagaagcaa tcattaaagc aacagatgca aaaccaggtg acttgctgat gtttggcgca
    30541 gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc ggacacgttt aggaaaagaa
    30601 ttaggactaa ttgatgaatc gaaattcaat ttcttatggg taactgattg gccacaattt
    30661 gaattttcag aagaagaagg tcgatatgtc tctgctcacc atccattcac gatgccaaaa
    30721 gaagaagata tcccgttatt ggctgaagac cctgcaaaag tctatgcaga agcttatgat
    30781 atcgtcttga acggttatga actaggcgga ggttctatcc gtattcatac tcgtgagctt
    30841 caagaaaaag tactagaaac tctaggcttt acaaaagaac aaatggaaga acaatttggt
    30901 ttcttgttaa cagctttaga ttacggattc cctccacatg gcggtattgc attaggattg
    30961 gaccgttttg caatgttatt agccggagaa gataatattc gtgaagtcat tgcctttcct
    31021 aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa gtatggtttc gcctttacaa
    31081 ttgttcgaat tgaatattga tgtaacgaat atagaagaat agccgataaa aaggtaaaaa
    31141 ggatttgtcg aatacgcttc ttttgacaac cttgatttaa ttgaaaaaca ctttataatg
    31201 aagccataac atataaagcg gggcttttcc atgaaaaaaa cattcgaaaa ttgggcttat
    31261 catgattaca cgaccaagct ttcggtatct atcgtttatg cgttgatcgc atcagtcgca
    31321 ttgaatttct tttatcagcc aggacatatt tattcaagtg gtattactgg tctagctcag
    31381 attttgacga ctttgtcaaa atcactgata ggattcgaag taccggtatc aacaactttg
    31441 tatttactga atataccttt gtttgtattg gcgtggttaa aaatcgggaa gaagtttaca
    31501 attttcactt ttatcacagt cactttgacc tccctttgta tccatatttt accggaaaca
    31561 gtcctatcta ccgatccaat catctgtgcg ttgtttgggg gggctgtgat gggactaggt
    31621 attgggctag cgttgagaaa cggcctatca tcgggcggtc tagatatcgt gagtatcacg
    31681 attcgtaaaa aaacaggaaa gtcgattggt tctatttcga tctacttcaa tgcattgatc
    31741 gtttttgttg ccggaattct atttggctgg caatatatgt tttacagtac gttgtctatt
    31801 ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac aaaagaagat gcaggtaatg
    31861 atcgtaacga aacatccaga aaaagtagta aatgaaatcc aaaactgtat gcgtagaggg
    31921 atcacgatca tcaatgaagc agaaggtgct tataagcatg ataagcagac agtattgatg
    31981 acgatcgtta cacgatacga actgcctcta cttagagaag caatgaaagc ttctgatcca
    32041 caagcttttg taagtattgc agaaaacgtc cagatacttg gtcgtttcta tgaggatggc
    32101 atttagatcg agtttgacaa aaattcatga aatactgctg tagacagacg ttcgtttgtc
    32161 tgcagttttt ttctaccata gtagaatcag caaagaaaaa ctgctttttt atttgaaatt
    32221 ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt tatgggatga tagaaaaaaa
    32281 tagtagagaa aggacacgat tctacttgct attttcactt aatacccgta tattacgaat
    32341 gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa caaaaaaggt taaaaaacag
    32401 ccgctacatc actgggtttg atgggctccg ttcattagct gtggtgggtg ttatcttcta
    32461 tcaccttctg ccaactagta tgaaaggcgg ctatttgggc gttccgattt ttttcgtcgt
    32521 atctggttat ttgatcacgg atctgctgcg gcaggaatgg gaacaaaatg gaaaaatcaa
    32581 tatttggcaa ttttatgtca gaagaatgaa gagactctat ccggcacttg tttttatgct
    32641 tgtgacagct tcggcttata tcactttgtt tcagcgaggg ctgttaaaca atcttcgagg
    32701 agttgtggtc agcagcttac tgtatgtcaa caactggtgg caaatcaata acggtttatc
    32761 ttatttcgat cgatttgcta atgagtcacc gtttacgcat atctggtctt tagcagtaga
    32821 aggtcaaaac tatttggttt ggccgatctt gtttgtattg ttgatggtat ttgttcgtaa
    32881 aagaaaatgg atcttttata ctgtgttagg tacatccttc ctctccgcca tcttgatgat
    32941 gatcttgtat acaccaggag gcgatccaac ccgtgtttat tacggtaccg acactcgtct
    33001 gttttctata tggttgggaa gtgcgcttgc gtttgtttgg ccaagtacgc gtttaaagaa
    33061 aaacatcccg aaacaagcga aaaatattct gaatatcgct ggaggaatct cgcttttggc
    33121 actgattctt tgcttcttct ttttagatga tcattacaat ttcatctatt acggaggaat
    33181 gctcctagtg agtctgttat gcgtagtgct agttgcagtg acagcacacc ccggagcaag
    33241 tttgaatcga tggctgacaa atccagtatt tacttggatt ggcaaacgaa gctatggcat
    33301 ttatctgtat cagtttccag ttatgatttt ttatgaagca aaggtgactg atattgccga
    33361 tcatgttttg ttgcatactt tagtggaaat cacattgatt ctaatcatca gtgaactttc
    33421 ttatcgatat gtggagcgtc cgctagcacg ttttgattat ggtcgtacat tccaagtgat
    33481 gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa ccatgggttg ttccaggcac
    33541 attacttgta gcaattgcac tcgttggttt cgttacggca ccgaaaaacg ctgtaacagc
    33601 cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa aaattagcag aagaaaccag
    33661 aaacaattca gaaaacacca ctagttcggt tgaccaagcg atccttgata agtacgagtt
    33721 gtccaaggca caagggaata aggcacaaaa attagaacta acggcatttg gtgattcagt
    33781 tatgttggat gctgcaacgg atctacaaga actttatcca aaagtagtgg tagatggaga
    33841 tgtcggacga caattatatg ccagtgtacc gtacattgaa aaattaaagg aacaagattt
    33901 gttgaaagac actgttctga tcggactagg aacgaatggc tcattctcgg aagctcagtt
    33961 tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac tgggtcaatg tccgagtacc
    34021 gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa cagatgacga aaaaatacga
    34081 caatatgacg ctgattgatt ggtacagcta cagtaatgac catagtgact ggttttatga
    34141 tgatcaagtc catccaaatc ctgaagggat gacacattac attcacttag tcagcgaagc
    34201 cttgctagga ggaagttcaa gtacgaacag cagtgagaca aacgtgacag aatgatagct
    34261 acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag tttttttata tttaccgtgt
    34321 ttttaacatt catatagatt caggaagcaa aacggttgag tgtgtctgta aataacgttc
    34381 tttttgtttt cgggtgagtt ttttgaatgc agcttctgct tttgttgctt cgcttcttga
    34441 agaaaatact tctgcatgga tcatcttaac aggtcttctt ttttgaagac gagtatattt
    34501 agcaccagta ccattgttat gttcctctaa tcgtctagaa agatcggttg tatatccacc
    34561 ataaaatgtc tgatctttac agaataatac gtaaaaataa tgttcatttg ccatagagca
    34621 tctcacgaat ctctgcagtg tattctccat ccatattgta agtgatcaat ggtggagcga
    34681 ctttgaatcc gtctaatttg ccttctttga ttccttcaac taacaaaata tttgcctctt
    34741 ttccaggttt tgggtaaaca aattggatcc ttttaggtgc aattcgatag tcttccatga
    34801 cctgaatgat ttctaagaac cgatcaggac gatggaccat agtaaagcgt ccatttgttt
    34861 ttagtagctt acttgaaata tcgatcactt cttctaacgt cgtatgaatc tcatgacggg
    34921 cgattgccag gtgtttatta gggttcgttt tatttgttgg cagccctttg aaataaggtg
    34981 gattacagac gactaagtca caagaattat gtcgaacgac agataagctg tctttcagat
    35041 cgatggtgtg gacagtcatt tgttcattta aatggttaag cgcgatacta cgtcttgcca
    35101 tgtctgctag acgaggttga agttcgatag cgtcgatagc cgcctgggtt cgtttactta
    35161 aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac aatcttccct tttttaggta
    35221 cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa agaaaacact ttggaacttt
    35281 ggataatctt gatatcttct gcgtataatt ggtcgattcg ttcgtcttcg tgaagcatgg
    35341 attccctcca aaatgaaaaa aatagtcgtc tgtattatac tatttttatt aaaaagaacc
    35401 aaggtggctt gtcaaacctc tattttttat gcatactatg accataagaa gggaaaggaa
    35461 gagactatgt tttatcgatt tatgcgcggg gtagttcgag tactattagc cttagttaat
    35521 ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag atgagaatta catactagtt
    35581 gctccccatc gtacttggtg ggacccatta tatctggcgg tagctgcagc acccaaggaa
    35641 ttcagcttta tggcaaaaga agaactgttc aaaaatccag ttctgcgatt tatattaact
    35701 cgatccaatg cttttccagt taaaagagaa aatccaggac caagcgtcat taaaacacct
    35761 gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt ttcccagtgg gacaagacat
    35821 gctacagaac ttaaaggcgg aatggcattg atttccagaa tgtctaaagc gaaaatcgtg
    35881 ccagctgtat atcaaggtcc gttgacactt ggggatctgt tcaagcgtca gagagtgaca
    35941 gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga aaacggataa agaaggtatg
    36001 gcggagatcg aacgacgaac acaagcagct tttgacaaat tggacaaaga agtgaaccct
    36061 gatttcaaat atgaagtcaa aaaaaaataa aatgataagt ttatgcgtgg gcggtctttt
    36121 gaatctactg tcctgttcag ttcagacttt ttcaatgaaa acgagggaga gacgtcaagt
    36181 ctgccctctt tttgcgttcc aatatgtaaa atagaagaca ggtgtcatgt aatggatcaa
    36241 agtgttataa taattagaag gacatttttt gaggtgattg aatgcgtttt ttacatacag
    36301 ccgattggca tatcggaaaa aaactacaag gttatgactt gattgaagac cagcgtcatg
    36361 tactatcttc tatactagat atcgcgaaag aagaaaaagt agaggcaatc gtgattgccg
    36421 gagacttata tgataggagt gtcccagctg ttgatgccgt agaattatac aatgaattga
    36481 tggtcgattg gaatttaaag gaaaagatcc cgatttttgc tatatcagga aatcacgata
    36541 gcagtacgag actttcagcg ggtacgccat ggttcagtta ttcgaactat tttttgcgaa
    36601 cacagctagc agaagcattt gaaccagtcg agtttaaaaa cgtacaattt ttctttcttc
    36661 cttactttga accaattgca gcgagattat attttgaaga tgacaatatc cgaacgatca
    36721 agcaagcggt agccaaggtg atagataaaa tgcaggagct atttgattca gaaaaaaaac
    36781 aagtattgat tagccatttt tttgtaatgg gaagtgtaaa atctgattcg gaaacgaaaa
    36841 tcgaagtagg cgggttggat tcgatccctg gtgaactact tacaccgttt gattatgtag
    36901 cactaggaca tttgcataat caagctgctt tgagacagga taatgctcgc tatagcggtt
    36961 ccccattgaa attctcttta tctgagatca accaaaaaaa aggcgtatgg atcgttgacc
    37021 tgacaacagg cctggagttg aattttaggg agatcgaacc cctgcatgat attgaagaaa
    37081 taacagcgag tttcaaagaa ttgctggatc ctgcctttta tcagtccgtc aatcgggaga
    37141 attacatcta tatctatctc actgatagaa cgatcatccc gaatatgatg aaccagcttc
    37201 ggcaaatata tcctcgtatt ttggggattg aacgggcaaa cggtcgagca gaacaaacaa
    37261 agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa attagcagaa gcatttttta
    37321 gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa atggctcgga gaaacgttgc
    37381 aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa aaaattacaa cttaaaaatt
    37441 ttggtccttt tatcaatgag accgtagatt tttcacgttt gacagaagca ccgctttttt
    37501 tgatcagcgg gaagacagga gctggcaaaa cgacgatttt tgatgggatc accttcgcac
    37561 tttttggtga aacttccgga agacttcgct ctggaaaaga gatgcgttct ttgtttgcta
    37621 caccagacga agaaacaagc gtgacttttt cttttgaaca tcagcagatg agctatgaaa
    37681 tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg aaacggaaca aaaaaacagg
    37741 cagccaaagt aacgttgacc atttttgatg ggaaaggaaa agaaataaaa caaatcacaa
    37801 aacgtacaga agcagatcag ttgatcagag aactgatgaa tctcgatgcg aaacagtttt
    37861 ctcaaatcgt tttactaccc caaggagaat tccgtaattt tttggtttca tcaagcagtg
    37921 ataaagagac tgttttacgt catttattcg gtactcaatt tttccaacag ttcaatgagc
    37981 gattaaaaga aaaagcaaaa caacagcaac aaaaattgga tcatttagag caagagctgc
    38041 atcttctgca aaaaaggttt gttttaatca aagaagagga accagcagca gggtttgaag
    38101 ccgttttagc ccaatggaca aaccatcagg caatactcga aaaacaaatc acggaagaaa
    38161 aagaagctct aacggaactt caggaaaaac aaaagcaact agaaaattcc tactatcgtt
    38221 ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt aataaacaag caaaaggaat
    38281 tagctgctca aaaagacgaa atagaggaaa agaaacgatg gattgggtat tatgaattta
    38341 cgcaacgatt agtcgaacca atccagcgcc tacaagaaat aaaagttgaa aaaatagaac
    38401 tgtcacaaaa aagtagtgag caaacaaaga aactagcgat gactacgcag aattatgaag
    38461 agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac aaaaatcaaa gaagctgctc
    38521 aaaaactgca aaacaaacag atgatggtgc caattgtcga atccttacgt agtaaaaaac
    38581 aagacgcaga acaattaaca atttcattgg ataaagaaga attgaatgcc gagaagctgt
    38641 atcaaaatct cgctatctat caggaaagaa ctcgaattct tcaagaaaat ttactggatc
    38701 aggaaaaact gcaagaagat cgtcttgctt ttggacagtt aaaatatcta gatgaaaagc
    38761 agcaagaaat agaaaaacaa aaagcaaaag acactgggtc gctcgcacag ataagagaag
    38821 aactcaaaaa agcaactgaa gaaataacaa aaaatagtca gctattgaaa gaacagacag
    38881 aacaagcaga acagctgaaa agccaatggg caaagcagca aattgctcgt cttcagcttt
    38941 ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac ggaacacccc atacaacaat
    39001 taaaacacga acagccaaat aaggaagata tcttaaaagt agaacaacaa ctggcagaag
    39061 ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa aagtgaagca catgaaaata
    39121 atcttcaaca acaattatca gatttagaac aaaaaatcat ggcaacgaat atacaattgg
    39181 ctgaacaaga agaacacttg caacaggctt tgaaaaaggc atttcctgag ataacagaac
    39241 aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt agatagacga aaaagcaaga
    39301 tggccaatgc aagaatagag ctggaagaaa taagacaaca agcagaaaag ctcgaacaac
    39361 aattagctgt ctacaaacag cagcagacag aaaaaaataa tcagctccag caactgcaag
    39421 gagaaatcaa aagtttagaa caacaattgc aagatacgga tagtaactgt ttagaagagg
    39481 agatacgacg acttgaaaaa cagttagaag aagactcgaa tcaactagct gttgagcaga
    39541 aagcaggagt gcaattgttg caagatcttc atacgcttcg gacacagctt gatcttcaac
    39601 atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga acaagaaaga atagctaaaa
    39661 agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat ctgtgcattt gcacaacagc
    39721 aagcacaata taaagaaaac caacgtacag ttacttctta tcatgaagag caattagtga
    39781 cagctgaccg gctgcgtcaa ttagaaaata tccagctaac tgagacattc ccagatacag
    39841 aagagatcag ccaacagtta gaagaaataa gacgtatgat cgaaataaag caagaaaaga
    39901 tatacggttt acaagaacaa aaaattagta atcaaaaaat ctacgatgaa tgtctttcga
    39961 tttatcacac aagccagacg aaattagatg agcttacgca attacagcag ctttcgcaaa
    40021 cattcaacgg agaaaatcct aagaagacta gtttggaacg gtatgtgctt caggtttatc
    40081 ttcaagaagt gttacaagta gcaaatgatc atctccaacg tttaacgaaa aatcgttatc
    40141 aatttgagct agcggatacc attggcagtt atcgagggaa aaccggatta gaaatcaata
    40201 tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac cctgtctggc ggtgagagtt
    40261 ttattgcagc actttcttta gcattagctc ttgccgatgt gatccagaca caagctggtg
    40321 gtgtagcaat cgatgcattg tttattgatg aaggatttgg gtcattagat gaagaagctt
    40381 tggaaatggc aatggaagct ttagaaacga tcgaaagtga aggaagaatg atcggcatca
    40441 ttagtcatgt aagagaattg aaagaacgag tattacaaca aataagaatt gatacagaag
    40501 gtagcggaca aagcaaaata cgctatcaac ttggataaaa agggagattg gtaactatga
    40561 aatggagtat tccagaaaaa gtcattgcac gtggacgtca atacttgcaa gaaggccgag
    40621 tgttatcggt tgtccctgac caagaagaaa acgtgtggca tgcagaagtc ttgggcagtg
    40681 aattgtatat ggttgatttg gatggtacag caaaagagat agattactgc caatgtccct
    40741 attgggagga acataaatat tgtaagcata cagttgctgt ggaactttat ttacggtcga
    40801 aaaatctatc tcgtatcatg aaaaaagacc agccaatgct agaaccggta aaagcaacaa
    40861 gtgagggaga aatcattgca aaaggttttt ctcgtctaaa aacacaaaac aatcctcaag
    40921 cggtgcaacc tttgattatt gaataccaag tagaaacgat tgagactaat cagtatcacc
    40981 cagagctttc tattttggca ctttacttgc gtatcggttc attggcatca ccgaaaaaaa
    41041 catatatcgt aaaaaacatc tatgattttt tacaagctta cacagaaaaa gaaacttaca
    41101 ctgttaataa acagtaccag tttcgtctag ataaacgggc gtttcaaaaa gaagatcaaa
    41161 aaatactcac gcatcttgca ggaagtgctc agacgcatca attattaggt acgaacggct
    41221 tacaagtcaa aggaaaacta gataaaagat atctgcttct cccagtagaa caagcccgct
    41281 tcctgttaga gcagatgaat cagactacac gtttgcatct gcagataaat gaacaaaacg
    41341 atcaaggaat acaattttcc gatcaaaaca aacctttatc attcaaagtg gaaaaatttg
    41401 gagaagatta tttgctgcaa gcattgaatg attttgattt tttctttatg cattatcagt
    41461 ggggagttat ccaagggaca ttttattctc tgacgaaata tcaacaaacg gtttatcaaa
    41521 cttggaaaca attgattcgc cgattggaga aaccagaagt agtgttcaag aaaaaagaat
    41581 taccgtcttt gtttaaagag attatccctc tgctgtcaga gatcggcgac gtagctattg
    41641 cccctgcagt agaagaggaa gtagcagata tccccgtcga atttattttc tttttccgaa
    41701 aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta tggcgaagtc atctattcga
    41761 ctgacccgaa gcatgaggtc tcgacagaac aggcttttca ggtgttacgt gacacagtag
    41821 aagagaaacg agtacttgat ctattcagaa tgtatcgcta ccagaaaaat gaaactggtt
    41881 atgaacgtcc attgccaagc ggggaagaac tatatgcctt tttccgcaca gaactatctg
    41941 tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt gcgagagttg tatttagatg
    42001 cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg ttcatggctc gatattcgat
    42061 tcgatgtgac agggatagag gagcaagaaa tcgatcatgt cctgcaaagc ttgctgagaa
    42121 atgatgcttt ttatacatta gaaaatggtc aggttctttc ttttgattca gaagaatttc
    42181 aacaaacaag ccaagtgttg caacaattac gggaatcgat acgtacagaa gaaggaacga
    42241 tccatgtacc taaaaatcaa ggattgatca ttcaaaatca attggaaaaa agcaatgcta
    42301 cttttagtga atcgttccaa acgatggtac aagatctcat tcatccagaa agataccagg
    42361 cacagcttcc caaaggattg aatgcaacga tgagagatta tcaaaagcaa ggttttcgat
    42421 ggttgaagat gttaggtcat tatcagtttg gcggtatttt agccgatgaa atgggacttg
    42481 ggaaaacact acagacaatt gcctttttac tttcggaaaa agaagaacgg aaaagcttct
    42541 ctgctttgat cgtagcgcct gcgagcttaa tttacaactg gcaggcagag gtgagaaaat
    42601 tcgccccttc tttaagtatc caagtaatca atgggaataa aaaagaacgt gaagaattac
    42661 tggctaaaga cacagatatc cgagtgactt cttatgccag cttaagacag gatctagcta
    42721 attatcagtc gcaaaaaatt gattatttga ttcttgatga ggcacaaatg gtcaaaaata
    42781 gcagtacgaa aactgcacaa gcattgagag aattagctgt acctcagcgg tttgcactga
    42841 gtgggacacc aatcgagaat aatttagaag aattgtggtc attgtttgca acgattatgc
    42901 caggattttt cccgactaaa acaaaattca gggaacttgc cgcagaagaa attgcgcaaa
    42961 tgattcgacc gtttatttta cggcgagata aacaaactgt gttgaaagac ttgccggaaa
    43021 aaacagagat gaatctatat tctgcactta cagaagaaca aaaaactgtt tatttagctt
    43081 acttgcgtca aatgcgtgaa gaaatcacat caatggattc tgaagcattc aagaaaaacc
    43141 ggatcgggat acttgctggt ctgacaagac tgcgacaaat ttgctgcgat cctcgtctgt
    43201 ttattgaaga ttaccaaggc ggttccggca agctcgaaca agtaaaagat ctactggtag
    43261 ctgccaaaga gaacaaacga agagttttgc tgttttctca atttacaggg atgctgacta
    43321 tcttgcaaga agagttagcc gagctaggga tctcgacatt ttatttaaga ggcagtacga
    43381 aaccacaaga tcggttaagt atggtagacg catttaatgc tggcgaaaaa gatgtttttc
    43441 tgatttcctt aaaggctggg ggaactggat tgaatctcac gggagcagat acggtcatct
    43501 tgtatgacct ttggtggaat cctgccattg aagaacaagc tgctggacgt gcacatcgga
    43561 tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc tgaaggaacg attgaggaac
    43621 gcatggattc actccagcaa gaaaaacgcg aactgttcca aaaagtgatc caaggtaatg
    43681 aagaacagtt gacgaaactg actgaagaag acatccgcat gattttaagt gtaggagaaa
    43741 tcgaggagta agagactcga aaaagcgttc agtaatgggt aagaaagcct ttctaaagaa
    43801 gcgattgttt atttgattta aaaaatagca aggaggctgg gattccattt tatcccagct
    43861 tccttgctat cattttttta tttctatttt taaaggttat acaaagattg tcttcattgt
    43921 ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa attggagtct gttcaatttt
    43981 gcgttctcga tggttgaacc aaagcgcttg ccagttagca ctttttgcac ctagaacatc
    44041 attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga agcatatgaa attctttctc
    44101 agctagctga aaaatctctt tttctggttt ttggaaacct gttgcttgag aaatgagcat
    44161 attgcgggaa ggaatccaat tgttcaattg aagttgcttc agtttttttg tctgatggtc
    44221 agtcggccca tttgtgataa ttcctaatgg gacattcttt tccttcaaga aatcgagggt
    44281 ttttttgacc tcctcatgca agcagatatt atcaagttct tcctcgtaaa ttttttgaaa
    44341 taagaggcca tcttcttccg taatatgagg gtagtctaaa tcttttagtg attggctgat
    44401 ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc attacttttg ggaagttttc
    44461 atcactatga tggcggaacc ggatatatag tggatgcata tctgcatctg aaacaagcgg
    44521 aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga tcatacatcg tatcatcaac
    44581 atcaaacaca actgatttaa tcaaaatttt acacgccttt cggttttcat tgattttttc
    44641 acaaatcctt gactattgta acagaaaaaa ttgtctaaaa aagctgataa ataaaggttt
    44701 ttcttactat atttctattt ccttgacaga ataaggtttt tatatttcag tttttttcac
    44761 taaaaatcgt gaaacacgtc aataagtttg atgaacaaat taatcattat ggtagaagtc
    44821 gattgattta ttttaatgca atctctgtgg aattttcgaa agatttattt ttttaataca
    44881 gtctcaatat aactattcaa acataaaata acgttatttg atttatttgt ttatagtaaa
    44941 ttttatttag taatcattgc tttaaaatac tacgcttata taacagagaa aataagcgtt
    45001 ataataaaga acagtattat ttaaaatgac agaaaacatt actattaaag aaataaaaaa
    45061 cataaaaaat cttaagaaat cggtttcatt tagcttttga gataaaataa tagtacagtt
    45121 ttatacgctg tttcatatgt ttagaaaatt tgaattagat ttgaaaactc taatttttaa
    45181 atgataaaaa aagcgagaat gctggtttta ctgtatatta accttagata actttcattt
    45241 ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt tgattatgta taatagaaac
    45301 agatgggaga ttattatgat cttctgttac aaaaaagtga ttcttatcac gaaaaggagt
    45361 ggtgcacgtt gttaacatta tatacttccc caagttgtac ttcctgcaga aaggctcgtg
    45421 cgtggttgca agaacatcag attccatttg ttgaacgtaa tattttttca gaaccattga
    45481 acagttctga attaaaagcg attttacaaa tgacagaaga cggaacagaa gaaatcatct
    45541 cgacacgttc caaagtattc caaaaactga acatggattt agatgatctg ccgttacaag
    45601 aattattgga acttgtgcaa aacaatcctg gattgcttcg ccgtccaatc atgattgatg
    45661 ataaacgtct gcaagttggt tttaatgaag atgaaattcg tcggttccta ccgcgcgatg
    45721 tacgacaatt agaattacgt caagcacaat tgatggctgg attatagaag gataatacga
    45781 aacttggaaa agactattac aaagatagtt tttccaagtt tttttgtttt tacaaaaagt
    45841 attcttgatt agatgtataa aaag
//
LOCUS       DASOUD010000008        43751 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.008, whole genome shotgun sequence.
ACCESSION   DASOUD010000008 DASOUD010000000
VERSION     DASOUD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.008"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(53..787)
                     /locus_tag="U4A29_000467"
     CDS             complement(53..787)
                     /locus_tag="U4A29_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO0679616.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="U4A29_000468"
     CDS             1114..1524
                     /locus_tag="U4A29_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO0679617.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="U4A29_000469"
     CDS             1517..2218
                     /locus_tag="U4A29_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO0679618.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="U4A29_000470"
     CDS             2218..3804
                     /locus_tag="U4A29_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0679619.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="U4A29_000471"
     CDS             3823..4044
                     /locus_tag="U4A29_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO0679620.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="U4A29_000472"
     CDS             4041..4802
                     /locus_tag="U4A29_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO0679621.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="U4A29_000473"
     CDS             5182..5691
                     /locus_tag="U4A29_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO0679622.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="U4A29_000474"
     CDS             complement(5766..6305)
                     /locus_tag="U4A29_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO0679623.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="U4A29_000475"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="U4A29_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO0679624.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="U4A29_000476"
     CDS             7318..8169
                     /locus_tag="U4A29_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO0679625.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="U4A29_000477"
     CDS             8197..8832
                     /locus_tag="U4A29_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO0679626.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="U4A29_000478"
     CDS             8852..9625
                     /locus_tag="U4A29_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO0679627.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="U4A29_000479"
     CDS             10033..10830
                     /locus_tag="U4A29_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEO0679628.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="U4A29_000480"
     CDS             10808..11221
                     /locus_tag="U4A29_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEO0679629.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="U4A29_000481"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="U4A29_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO0679630.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISSVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="U4A29_000482"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="U4A29_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO0679631.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="U4A29_000483"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="U4A29_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEO0679632.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="U4A29_000484"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="U4A29_000484"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO0679633.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="U4A29_000485"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="U4A29_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEO0679634.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="U4A29_000486"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="U4A29_000486"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO0679635.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATEQPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="U4A29_000487"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="U4A29_000487"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO0679636.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="U4A29_000488"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="U4A29_000488"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO0679637.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="U4A29_000489"
     CDS             21905..22483
                     /locus_tag="U4A29_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO0679638.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="U4A29_000490"
     CDS             22535..23017
                     /locus_tag="U4A29_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO0679639.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="U4A29_000491"
     CDS             23134..23508
                     /locus_tag="U4A29_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679640.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="U4A29_000492"
     CDS             complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="U4A29_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO0679641.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25685..26275
                     /locus_tag="U4A29_000493"
     CDS             25685..26275
                     /locus_tag="U4A29_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO0679642.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26403..27752)
                     /locus_tag="U4A29_000494"
     CDS             complement(26403..27752)
                     /locus_tag="U4A29_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0679643.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27920..28660)
                     /locus_tag="U4A29_000495"
     CDS             complement(27920..28660)
                     /locus_tag="U4A29_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO0679644.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28673..30265)
                     /locus_tag="U4A29_000496"
     CDS             complement(28673..30265)
                     /locus_tag="U4A29_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO0679645.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30833..31129)
                     /locus_tag="U4A29_000497"
     CDS             complement(30833..31129)
                     /locus_tag="U4A29_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679646.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31305..32030
                     /locus_tag="U4A29_000498"
     CDS             31305..32030
                     /locus_tag="U4A29_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEO0679647.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32023..32865
                     /locus_tag="U4A29_000499"
     CDS             32023..32865
                     /locus_tag="U4A29_000499"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO0679648.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32868..33389
                     /locus_tag="U4A29_000500"
     CDS             32868..33389
                     /locus_tag="U4A29_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO0679649.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33642..34205)
                     /locus_tag="U4A29_000501"
     CDS             complement(33642..34205)
                     /locus_tag="U4A29_000501"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO0679650.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34456..34725
                     /gene="rpsO"
                     /locus_tag="U4A29_000502"
     CDS             34456..34725
                     /gene="rpsO"
                     /locus_tag="U4A29_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO0679651.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34913..37039
                     /gene="pnp"
                     /locus_tag="U4A29_000503"
     CDS             34913..37039
                     /gene="pnp"
                     /locus_tag="U4A29_000503"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO0679652.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37549..37815
                     /locus_tag="U4A29_000504"
     CDS             37549..37815
                     /locus_tag="U4A29_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO0679653.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37992..39950
                     /locus_tag="U4A29_000505"
     CDS             37992..39950
                     /locus_tag="U4A29_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO0679654.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40304..41605
                     /locus_tag="U4A29_000506"
     CDS             40304..41605
                     /locus_tag="U4A29_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO0679655.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41735..43105)
                     /locus_tag="U4A29_000507"
     CDS             complement(41735..43105)
                     /locus_tag="U4A29_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO0679656.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43287..43610)
                     /locus_tag="U4A29_000508"
     CDS             complement(43287..43610)
                     /locus_tag="U4A29_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEO0679657.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
BASE COUNT        14163 a         8127 c         9111 g        12350 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agttcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacaaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcaataga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgac gaaaataaaa taacaaacaa tttgtaaagt tgacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa aatttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcgaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacgattat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcacagcatg acagcattgg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ctagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt ttgcgaaaac tatttttata tcatatatat gtctaaaatc tatattataa
    23881 aagaaatgtt gtttgatcag cttctcttcg gtattagttc aaaacaaaac ggcaaagtat
    23941 atggagctac tttgccgttt tgtttctcta atactcaggt cagtttatag acgctcaata
    24001 aacggtgttc aaaagccagt ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag
    24061 ccattttcaa aaaatatttc ttaatactcg aaactgagcg gctttttcac gacctctcaa
    24121 taaacagtgt tcaagagaca gcctatcggc aataaggctg aacgtctctt tcacaacctc
    24181 ttttatcaat ctgctacttc ttctttctta gcgaaactcc aaatcagtcc gataacaaaa
    24241 ccgataactg ctggtacgat ccaccccatc cccaatttga aaaacggcaa gtagctttcc
    24301 gcaacatata aaagattttg aacaataggt gtttgtttga tagtttctgg gcaagcgttc
    24361 aacccatcaa aaatcgaagc aatcaatgta aaataagtag tcattcgata aacagccgta
    24421 cgctgcttaa acaaaggacc aactaacact agtaaaatca gtgtcattgc taatggatag
    24481 atgaacatca aaacaggcaa cgaaaattga atgatattcg tcaagcctac gtttgccgct
    24541 aaacaaggaa ggatactagc aagtgcaaca aagaatgcgt aatttctttg cggaaacaaa
    24601 tctgtgaatg tttctgcaaa agctgtaatc aaaccaatac cggtcttcaa gcaagctaat
    24661 ataacgataa atgctaataa gatactgcca taagtaccta aataatgatc agcaatctga
    24721 gccagcgtga tcccgccatt ttcacttaga gcaaagctac ctaagctcat agcacctaaa
    24781 taagcgagca aagtataaat gattcccatc aatataatgc tgatcgcacc agatttgatc
    24841 gtgtctttag cgatttcagc aggttttgtt acacccatgt tttggattgt tgtgacaatg
    24901 atgatcccaa atgctaaagc agcaagcgca tctaatgtgt tatatccttg agtaaaccct
    24961 gtgaaaaaag catgctcttg ataatttggc tgaataggcg cttgatttac agaacctagt
    25021 ggattcaaaa aagcaaggag aagcaaagca cctaataaaa ccaaaaatgc aggattcagg
    25081 aactttccaa cataatctaa tatctttgtc ggttttcttg aaaaccacca agccagcaca
    25141 aaaaatacaa tactgaaaat tgctagatat agcgtctgtc tgctaggatc gataaatggt
    25201 gctaaaccaa tctcgaaaga tgtggttgca aggcgaggta aagcaaaaaa cggtccgata
    25261 actaagtaaa gcaagatggt aaaaaccatc gcatatgttt gatttatccg ttttgctaat
    25321 tcatatacac ctgagctttt cgatacacca atggcaatca cccctaagaa gggaagaccg
    25381 ataccggtaa ccaagaaacc tagattcgcc caaaaggtag cagcgcctgc ttcttgaccc
    25441 aaatgaacgg gaaagataag atttccagca ccaaaaaata gaccgaaaag catggagcct
    25501 ataaaaagat agtctttaaa tgataatttt tttgtcatac ataactcctt tacggttttt
    25561 ttaaaagtaa caaaatttcg aaaaatatgc aacaattttc agaaaatttc ctatataaaa
    25621 cgacggttat ttaataacgt aagtgataaa ataaaagcgc tgaatcatct gaggaggaat
    25681 catcgtgaca aagtcgagat tagaagcatt tactgatgga gtggtagcca ttgttttgag
    25741 agttcttgta ttagagatca agatccctaa tacatctagc tttcattctt tatgggtcat
    25801 ccgaaataca ttattagctt ataccatcag ctttatattt gttgcggtga tctgggtgaa
    25861 ccatcatcgt atatttcaga tggcagaacg aattaattac cgagtggtct ggtcgaatat
    25921 ctttttgctc ttttggctga ctttatgtcc ttctgttact agttgggtcg ggcgtaaccc
    25981 tgaagctttt tggcctgaga tggcttatgt cttagtatac acgatgtgga gtttttcttt
    26041 tggtatcttg atgaggcaaa tcatgaaagc aaataaacca gattcccatg tcgtgagagt
    26101 tttgtcaaag gaccgtcgaa gtcttatctc catgatgatt aacttatgct tgattgccgg
    26161 tgtctttttt gtaccgttga tcgggttatt cggacgtttt tttgtatctg gtatttggat
    26221 tttttcctat aaaaaagcag atgcctatta tcagcggttg tttccgggga aatagaaaag
    26281 aggctgggac agaagctttt aactccaaga aataagccga aatttcacaa aaatttaaga
    26341 agcaattttc gtaaattcct tcttatttct cgaagctgac cacttctgtc acaactactt
    26401 tcttacttct tacgttcata tttcatatga tagattagta atagaagcaa gaaccacaca
    26461 ggtgttagta ataaagcgat tctcgtatct tttcctagga acaagacgac acacacaaag
    26521 gccatgtaaa gaaataccag ataattacta aatgggtaca acggcatttt aaatgaatga
    26581 gcttttttgc cgaggatact tttacgatat ttcatgtgcg tgtagacaag catactccag
    26641 ataaacaaga aacaagtagt ggcaatactg cttactagtg tgaatacttc actaggcatc
    26701 acgtaattca agataacaga aatgaaaatg acgatagtag aaaatgtcaa agcattccct
    26761 ggcacatgat gtgttgttaa ctttttgaat tttttagggg cactgccttc ttgcgctaaa
    26821 gaacgaagca tacgtcctgt actatatatt gcactgttgc aggcagaagc tgctgcactt
    26881 aatacgacta aattgatcaa ggaagcagca atcgtgatcc caatttccga aaatacttct
    26941 acaaatggac tgttatctgg attcaagctg ttccatggat agattgccat aatgacaaaa
    27001 agagatccta agtaaaataa gatgatcctg ataggaatat tattgatagc ttccggcaac
    27061 actttttcag gattttctgt ttctccagcg actaatccaa ctaattctat cccagcaaaa
    27121 gcaaaggtta ccatttggaa agctaagata aatccttttg caccagttgg aaagaatccg
    27181 ccatgagacc atagattcgt gatactggct gtgccaatat ctgtcgtata gtgggtgagg
    27241 atcatataag ctcctacgag gataaaggca atgatcgcta caactttgat caatgcaaac
    27301 caaaactcta actcaccaaa caatgagaca gccaccatat tcaagcaaag aagaagacct
    27361 aatgcaatca gttcaggaag ccactgagga acaccaggcg cccagtagcg tacatacatt
    27421 ccgatggctg ttaaatcggc cattgcaatc gatacccagc agaaccaata cgtccagcca
    27481 gtgatgaaag ccgctttttt gccaagatat tctgcaacaa aatccagaaa agaatggtta
    27541 ttggtgtttg ataacagaag ttcgcctaac gcacgcataa tgaaaaaaca gatgcatcca
    27601 gtaataagat aggcgagcaa tatcgaaggg ccagctagct ggatagactt tccggaacct
    27661 aaaaacaacc ccgttccaat cgctccgcca atagaaatca gttggacatg tctatttttc
    27721 agtccacgct gcagttcttg ctcttcattc atttttaaat catcctaact aaaaaagttt
    27781 aattacttcc atattaaaag agttttctga aaaacacaac taagattcca aaattttcag
    27841 aaatttacct tttatgagta attcataaaa aagaactgcg gtaaacaccg cagctctttg
    27901 tcccaatcag ctattgatcc taagcattta aaatacgtga aaggaattct tttgtacgtt
    27961 cttctttcgg atggacaaag atatcttctg gtgtaccttc ttctgcaatc acacctttat
    28021 ccataaaaat gacacggtca gatacatctt ttgcaaattc catctcatgc gtcacgataa
    28081 gcattgtcaa gccagtatgt gctaagtctt tgatcgtatt aagcacttcg ccaaccattt
    28141 cagggtctaa cgccgaagta ggttcgtcga atagcaatac atctggattc attgataatg
    28201 cccgagcaat tgcgacacgc tgtttctgtc caccagataa ttgtgctggt ttggcttcga
    28261 tataacgatc cataccaact ttttccagat tttcgattgc tacttttttg gcagtttctt
    28321 tatctcgttt caggacagtt gtttgaccag acatacagtt ttccaagaca ttcatattgt
    28381 tgaacaggtt gaaggattgg aaaaccatac ctagattcgt tcgatattta ggtaaattat
    28441 atttaggagc taatacattt tctccattat aaatgatctc gccgcctgtt ggtttttcca
    28501 gtaaattgat acagcgcaag aaagtagatt taccagaacc ggaagaaccg atgatcgtca
    28561 caacttcacc tttattgact gttacgctga tatctttcaa tacttcattt tcaccgaagc
    28621 tttttcttag atgtttgatt tcaataattt tttcattcat gattcttcct ccttattcct
    28681 gcaaatttga attgtcgatt tcttcaagct tggtataagc tgaaggacca tcgattttct
    28741 tttctaccgc ccgcaggatt ctggtgatcg taaaggtcat gactaagtag atgatcccga
    28801 cgatcgtaaa tgtttggaag aattggaaat tcgcaccgga agcagagttt ccttggaaga
    28861 acaaatcagc tacgccgatt acacttaaaa ctgctgtatc tttgatattg ataacaaact
    28921 catttcctgt tgctggcaag atattccgta aaacttgtgg aataacaact tttctcatcg
    28981 tttggccatg ggtcatacca atggcttgcg cagcttcgaa ctgcccttca tctactgcaa
    29041 aaatcccccc acgaacgatc tctgtcatgt aagctccagt attgagagag acaataaaca
    29101 aagcggcaat cgttcggttc aatgaaatgc caaaagcgag tgccagaccg tagaagatga
    29161 ccattgcttg taccatcatc ggtgttccgc ggaatacttc gacatagata gaaagaagtg
    29221 catttccgat tttttggaag aagcgagtca gtgcatttcc tgattcaggt accgaacgga
    29281 agacaccaat cagtaatcca agtgtagtac cgacaactgt cccgatcaat gcgatgaaca
    29341 aggtaagtcc agcacctcgt aagaacatat tgccgtattg tttccaaatc gtagtgaaat
    29401 cagaaatgat gccaggtttt tcttcactat cagttgcagc aggctgatct ttgactgctt
    29461 ggtccataat tttggttcgt tcatcgttag agatgccaga aagaatctgg tttacttttt
    29521 ggatatccgg gtcgccttta cgcataccaa cagaaatctg aacatcttcc gggtttgtct
    29581 ggaagccatc tgcaccgctg aattcaagca tttccaagtc tttgttcaca ctagttgccg
    29641 ttacaccttc cggacgttcg ctgacataac catcgatcat tccagaagct aaagctgttc
    29701 tcattgctga gaaattatcc attgcttgtt gtttctgcac atctggtatc tgatcgatca
    29761 cagtatagtg gaatgtattt aactgagcag taatctttgc tccagaaaga tctgctaaac
    29821 tttttgcttt tgcatatttg ctatcttttt gaactactac tactaattgt gattcgtaat
    29881 aaggatcggt aaaatcaatt tcttttcgtc gttcagcagt agggctcatt ccggcgatga
    29941 tcgcgtcgat cttgcctgat tgaagggcag gagccagacc atcccatttc gtttggacga
    30001 tgactaattt tttccctaaa ccatctgcta cttttttagc gatttgaaca tcataaccgc
    30061 cggcgtaact attttggcct tgaattggca aggcattatt ctcatctgtt tgttgagacc
    30121 agttgaaggg tgcataccca gcctccatac ctacgcgaaa ctcatcgctc ggtgtttttt
    30181 catctgcatt tgccagagat cccacactga atagtgagaa gacaaaagca aatagtacgg
    30241 caactgaaac catatttttt ttcatttttt tctccctttc tgattatatg atacacgagc
    30301 ggtctagatg aaatgcggta aagctttatc tgaaaaaata aaaagcgacc gatttataga
    30361 tataaaaagg ccgctaaaat tccataaggt gataaacctc tcacaaaaca tagcgcaact
    30421 tagcatcact gctaagacag tccgcaagct cttaacttgc gtcccaacat acactccaag
    30481 caaaaaagtg cacatttcgg cgatcctccc tattcctgct tctccatgtt tgcttcactc
    30541 cacagggtta atgattctcg cgacctctac ctcattgatt gaggcatctc gtattcagtt
    30601 atttatacaa aaaaagttta cgatactcga gaggctttgt caatctataa ctgctgtttt
    30661 ttgaaaaaat actgataaat atagaaatct ttacttttac cgaaaaaaca atcaaaatgt
    30721 gaatcatcta tccattgcaa acgatagtat aattatacac aaaaatgtat gtaatacaat
    30781 aaaaaaagaa ctagaacaga aaatttcctg tgtccagttc catctcgatc atttaagctg
    30841 ccgctttctt tggctcacgt cctaggatcg cttgcaaaag aacaacgacg ccataaatgg
    30901 ctaatattag ataaatcaaa cttgagtatt gggtgattcc ccagccccat gttcgatata
    30961 cggctaatgt aatggctaaa acaattgcgg caatcacgtt gaataaagca aaagcccaaa
    31021 gatttccatt ttctttgcgt acaagataaa aaacagagaa ataaacgctt aacgctaacc
    31081 aggcgataca agccagaata acgccccaat aaataaagaa tggtaacata tcgattcacc
    31141 tcacttttat acagcagtat tgtaacacgt tttcattctt ttgtcagtga acattgtgat
    31201 aaattgttca aatgcccata acactttttt attcccattt ttctgacttt tcaacaataa
    31261 atttgttcga gtatgggcta taatggaaag tgaggtgaca aaacatgaaa caattaacag
    31321 tagacgaagc agcaaaagag atattagctt atcagcaaat tacatttttg accggtgcag
    31381 gggtatccac accttctgga gtgccagact atcgatcttt gtcaggaatc taccacggga
    31441 tggagcagcc ggaatacttg ctgagtcatc aagcaatggt gaatgaaccg gataaattct
    31501 atcaatttgt caaaaagatt tatcatcctg aagcaaaacc caatatcatt catttggaaa
    31561 tggcacatct agcgaaagaa aaaaatgttt gggttgtttc ccaaaacatc gacggtttgc
    31621 acgcaaaggc taaaagcccg caactcgtca attttcatgg aagtttgtat caatgtcatt
    31681 gtcgaaaatg tcaacaaacg gtagactgga aagaatatct tcacagcgac aaacatcaag
    31741 catgcggagg acaaatacgc ccagacatcg tgttatatgg tgaagggttt caagatggag
    31801 tcatggagca ggcggcttat gccgtcagcc aagcagagct gatcgtcatt gttggaacga
    31861 gttttcaagt tcatccgttt tgtgatctgg tacagtttcg ccagccgtca gccaaaatct
    31921 tagtaatcaa tcaaacgcct gtttatttag cggagcctta tacgtttatt caagaagacg
    31981 gagcggttgt tttcaaaaaa atacaagaat taggagcaga ttatgactga attcaaaacc
    32041 gaaaaagaaa aaatgattgc aggcgagctt tattttgcca atgatcctga gctggttgcc
    32101 gaccgtatgt ttgcaagaag tcaaagccaa atcatcaacc aagcagaatc agcggaactg
    32161 cgatcccaat tgttgaaaga gacttttggc cgtacaggga aaaaaatcta tatggagcca
    32221 gttatcaatt ttgattacgg atataatata tttgttgggg agaattttta tgcaaatttc
    32281 aattgtacat ttttagatgt cagcacgatt gaaatcggtg ataactgcat gttcgctccg
    32341 aatgtccagc tttacacggc gacgcatcca ctacatccag tgaaacgtaa cagcggtctg
    32401 gagtacgcaa aacccattaa aatcggtaat aatgtttggt taggcggagg agttatcgtc
    32461 acgccgggtg tcactctagg ggacaacgta gtagtcggcg caggttcggt tgtgaccaaa
    32521 tcttttcccg acaatgtagt gattgcagga aatcctgctc gtatcatcaa gacagtagag
    32581 gaagaactaa cagaagagtc tcttgaaaca ttacgtcata agatcgatat gatcgatcga
    32641 gagttgattg ctttattaga aaaaagaatg gatacggtaa ctaaaatagg acaagtgaaa
    32701 aaagaagaaa accaagcagt ttatgatgca aaaagagagc agcaagtatt ggataaagtt
    32761 gttagtctgc tgaaaaataa gggatataag gaaacaataa cagatacgta tgttgatttg
    32821 atgaaacact cgcgcgaata ccaaaacaaa atgaaggagg aataagtgtg atcgaaggag
    32881 agacacggcg aaaacaaatc atgcaaacct tggcaaatac agaaaaacca gtgagtgcta
    32941 gcaagtttgc gaaaactttt ggagtcagcc gtcagatcat tgtaggcgat attgctttac
    33001 tacgagctgc aggtaaaagt atcgtggcaa cagcaagagg ttatatcctc gaaacagaag
    33061 aaaccaatac tgggcacatc agtaaaatcg ctgtccagca tggaaaagat caaacagaag
    33121 aagaactgag actaatcgtt gaaaatggtg gtgaaatcat tgatgtaatt gtggaacatc
    33181 ccctttacgg tgagttagca ggaactttgc acatcaaaac gccacaagat atcgattcct
    33241 ttatgagacg ttatcaaaaa agtaatgcta ctttgttatc cgaattaaca ggcggcatcc
    33301 atctgcatac gatccgctac ccagaaaagc atatgctgaa aaacatcaaa caaaaactag
    33361 ctgaagcagg aatcctctac gaaggataac atcgtagctg ctacttttta tcctagaaac
    33421 tattctatca tctctgagat aaacacccat atggaagaaa taagaaaaaa taagaaaaga
    33481 tgcgtatcca atacacagaa ttttttggaa ttgcgatttc tagatgtcta tctaaatttc
    33541 gattagtgga gaaacataaa aaagggaagt gcagcttttg agcactcccc ttttttataa
    33601 agctttcttg tagcatagta tcgaaggaac ctgccaattc tctactcgat caccaatacg
    33661 ccttctttta gtgcaaatgg attttgatcg ttgatgtggt catagaacat cacaccgttg
    33721 atatggtcga tttcatgttg aacgacgatc gattcataat tcttcaaacg aatcttgtgt
    33781 ttttcaccat tcatatcata ataagacaca gtgattttgg catggcgaac aacatatcct
    33841 ggtacttctc gatcaacaga taaacagcct tcaccttccc ctaaacaagc atcttgaaca
    33901 gaatggctca aaattttagg attatacata acagtactta gagaaggtcc atctgcctct
    33961 gggtcaggac ttggtacgtg aacagcaatg attcttttag aaatatctaa ttgaggagca
    34021 gctaagccaa caccgcctcg taagtgtagt tcttcagctt tgattggatc ttggctgttt
    34081 tttaagaact caagcatctc ttttcctaaa gagatatctt cttcgcttaa tggaagagaa
    34141 acttcttttg caacttcgcg taaggtcgga tttccttcac gaatgatatc atccattgta
    34201 atcatggaat atgtacccct ttcttaaaag actgtgtttc aagtctattt tcgcattata
    34261 gtttaccaca aaaatggaaa agtagtagta agattgtttg aaaactacct tttcttaatg
    34321 attaactact tgattttcaa cccggtttca ggtaaagtat agctgtgtgc tctaggcaca
    34381 tgacctgcca cttggttaag cgaatcacca acgttttact cagtcgcagg ggaataatcg
    34441 aaagaggtga aagaaatggc aatttcaaaa gaacgcaaga atgaaatcat caacgaatac
    34501 gctcgccatg aaggggatac tggttctcca gaagtacaaa tcgctgtatt aactgaagaa
    34561 atcaaccact tgaatgaaca tgcacgcgtg cacaaaaaag accatcattc ataccgcggc
    34621 ctaatgaaaa aagttggtca ccgccgtaac ttgttagctt acttacgtaa aactgacgtt
    34681 caacgttacc gcgaattgat caaacgttta ggcttgcgtc gttaatttta gtcaaaaaga
    34741 gtgggattca catgaatctc acttttttta ctcgtatatc aattaagtaa aattccgtga
    34801 tcaagctaga tggactccta ctaaccaaat gaaagctgct caaaagacgg ctttcggcag
    34861 ttttcatttg tttagtagcg tcttgctttc acgagaagga aaaggagaag ttatgtcaga
    34921 aaaacaagta ttcaaaacga catggggcgg ccgtccctta caaatcgaag tcggtcagct
    34981 agcaaaacaa gcaaacggag ccgttttagt ccgttatgga gatacagtag tcttaagtgc
    35041 agctgtggcc tctaaagaag caaaagatac agactttttc ccattaacga tcaattatga
    35101 agaaaaaatg tatgcagcag ggaaaatccc tggaggtttc atcaaacgag aaggccgtcc
    35161 aagtacggaa gcaacattga ctgcccgttt gatcgaccgt ccaattcgcc cgatgtttgc
    35221 agaaggcttc cgtaatgaag tccaagtaac gaacatcgtg atgagtgtag aaacagactg
    35281 ctcgccagct atggcagcaa tgctaggttc ttctttagca ctatcaatct cagatatccc
    35341 atttgacggc ccaatcgcag gcgtagaagt tggtcgtgta aatggcgagt acgtcttgaa
    35401 tccaacagta gaacaagcag aacaaacaga tatcgaattg actgttgccg gaacgaaaca
    35461 agccatcaat atggttgaaa gcggcgcgaa agaagtttca gaagaagaca tgcttggtgc
    35521 attgctgttt ggttttgacg ctatcaaaga gttagttgct ttccaagaag aaatcgttca
    35581 agcagtaggg aaagaaaaaa tggaagtgac tttactgcaa gttgatgaag tattgaaaaa
    35641 agaaatcttt gatgcttctt acgctacaat gaaagcagca gtaatgactg aagaaaaact
    35701 tgctcgcgaa gataatatcg agcaggtgaa gatcgatatc cgcgaagctt atgctgagaa
    35761 atttgcaggt catgaagatg aagatcactt gttgaaagaa gttaaacaaa tcacagaaga
    35821 tcttgaaaaa gatgtcgtac gtgaattgat caccatcgat aagatccgtc ctgacgggcg
    35881 taaattggat gaaatccgtc cattgtcatc agaggtcagc ttattgccac gtgtacatgg
    35941 atcaggtttg ttcacacgag gacaaacaca agctttatca gcttgtacac tcgcacctct
    36001 aggagagcac caaatcattg atggtttagg ggtggaagtc agcaaacgat tcatccacca
    36061 ctacaatttt cctcaatttt ctgttggttc tacaggacgt gcaggctctc cagggcgtcg
    36121 ggaaatcgga catggcgcat taggtgaacg tgcgttagca caagtaatcc cttcagaaga
    36181 agaatttcct tatacgatcc gtttagtagc agaagtattg gaatcaaatg gttcttcttc
    36241 ccaagcaagt atctgtgctg gcacattagc attgatggat gcaggtgtac caatcaaagc
    36301 gccagtcgcc ggtattgcga tgggacttgt ttctgatggt gaaaactata cgatcctaac
    36361 tgatatccaa gggctggaag atcatcttgg cgatatggac tttaaagttg ccggtacaaa
    36421 agatggtatc actgctttac agatggatat caagattcaa ggaatcacag aacaaatcct
    36481 aactgaagca ttaactcaag caaaacaagc acgtatggaa atcttagaag aattgacttc
    36541 aacgattgct gctccacgtg aagaattaag ccaatatgca ccaaaaatcg aaatgatcca
    36601 gatcgaacct gctaaaatca aagatgttat cggtaaaggc ggcgatacga tcaacggaat
    36661 catcgatgaa actggtgtga aaatcgatat cgatcaagat ggaaaagtaa gtattgcttc
    36721 agctgatgca gaaatgatca aaaaagctat caagatcatc gaagatttga ccaaagaagt
    36781 caaagttggt gaagtttatc tagggaaagt tgtccgtatc gaaaaattcg gtgcattcgt
    36841 caacttgatc aaaggaaaag atggacttgt ccatatttcc caattagcaa acgatcgtgt
    36901 caataaagtc gaagatgttg ttaaactagg tgatgagatc cttgtgaaag taactgaaat
    36961 cgataaacaa ggacgcgtaa atctgtcaag aaaagcaatg ttgaacgaag atggttcaac
    37021 gaaaaatgaa gaaaaataat ttaattcata ggaataatgt acttgtgatc gtgaaggtga
    37081 cagggggaca ataattattc gaaaaaaggg gactgtgaca aaagccgtgt cacagtcctt
    37141 ttttttcgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc agccaagtta
    37201 aaaacattag gagctgtttt tatttggctg ttcttattac ttgatgcaga acagcttttt
    37261 tacaacctct ccaataaacg atgttcaatc agctgtccct cgatattagc tcaaaaaacg
    37321 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggtca aaccgctgat
    37381 ttcacaacct ctgattcacg gtgttccaga agcagcttct acggaaataa gctgaacact
    37441 tttgttacaa cttctttttt tctggagttt cttggcgcat atttttggag tccttctgaa
    37501 taaagcgtaa aataaaagtg agaaatatgg ataaaggagg gaaaggagat gctgaatttt
    37561 ttatggtcgt tatttatcgg cggtatatta ggtttgatag ctggcgcaat tttaggaaag
    37621 gaagtcccgg gaggcgtatc tggaaatatc attatcggtt ttttaggtag ttggctcggc
    37681 gaatttttac taggaccact gggaccggaa ctcggcggtt tttatttgat accagcatta
    37741 ttgggtgcaa ttttttgcct tgctctttat tcatttgtgg caagacgtat cagagaacgt
    37801 ccaaagaaag aataatttta ttcaaataag ttgacacttc agtgtgttac ttggataatt
    37861 agaagcaatt taggaaagcg ggtgtcatgg ataagaatat catctatcat tcaagcttac
    37921 ctaaaggata tccatataac aagaaatgag ggaaaaacgt ggcaacaacg cacaatacca
    37981 aaaagatatc aatggctggc ctgcttgtag cgatgggagt cgtctacgga gacatcggga
    38041 caagtccgct ttacgttatg aaatcgattg ttgaagataa tggcggattg tcaggaatca
    38101 atcctgattt tgttatcgga tcagtctcat tgattttttg gacattgact ctactgacaa
    38161 caatcaaata tgtcgtgatt gcattgaatg cagataacca tggtgaaggt ggaattttct
    38221 cactctacac acttgtccga aaaggtggga aatatttgat cattcctgca atgatcggag
    38281 gagctgcctt actagcagat ggggtattga cccctgctgt gacggtaacg actgcgattg
    38341 aaggcttgcg aggaattccg cagttttttg accggttcgg taataaccag aacgtgatcg
    38401 tcattatcac tttagttatt atttttgttt tattcatggt ccaacgcttt ggtacagata
    38461 ttgtcggaaa agcattcgga ccaatcatgt tcgcttggtt tactttttta ggcgtaatgg
    38521 gattaatgaa cttcagtcaa gactggacag ttatccgtgc actgaatcct tattatgcta
    38581 tccagctgct ttttagtgca gataacaaat taggcttgtt cattcttgga aatattttcc
    38641 tggctacgac aggtgcagag gcgctttatt ccgatcttgg acatgtcggc aaacgaaata
    38701 ttcgtctgag ctggccttac gtcaaaattt gtttggttat caattatttt gggcaggctg
    38761 cgtggttgtt gaatgtttat caaaaccctg aagcacagca aatcaagaac ctgaatccat
    38821 tcttccaaat gatgcctcaa agttggatcg tgatcggcgt tgcttttgca acagtcgctg
    38881 cagtcattgc ttcgcaagca ttgattacag gttcgtttac gctagtttca gaagcaatca
    38941 agttgaaact gctgcctcgg ttaaagatca tgtatcctgg gaacagtatc gggcaaatgt
    39001 atatcccagc agtcaatctg attttatgga tcgcctgttc tctagtggtt gtcacattcc
    39061 gaacgtctca tcatatggaa gctgcttacg gtctatcgat cactgtgacg atgttgatga
    39121 caacagcact gctttacttt tatcttatcc aaaatgggca ttctcgttgg cttgcatatt
    39181 tagttacctt tttcttcggt gcgatagaag tcgttttctt tatctcaagt gttgtcaaat
    39241 tcttccgagg cggttttgta gccgttctga ttggtttgat catcttagtc gtgatgttta
    39301 tttgggaaca agggaacatt attcgcgaat cgtcaaccga ggatgtgtct ttgagagatt
    39361 atatcccgca gcttagagaa ttacgtgatg atacttcttt accaatctat cagacaaatg
    39421 tggtcttctt ggttcctgat atggctgaag ataaagttgg ccgacagttc atctattcga
    39481 ttttagataa acggcctaaa agagcacgag tctattggtt tgttcatgtg gaagtgacgg
    39541 atgagccata tacgaaagaa tatcaagtag atatgatgga cactgatttt attgtccaag
    39601 tgaatctgtt cttaggtttc agggtccaac aggaaatcaa cgtatatgtt cgccaagtca
    39661 tccatgattt gatgaaacaa ggccgtttgc caaaacagcc acaaagatac tctctgacac
    39721 cagggcgaga agttggcgat ttccaatttg tcctgattga tgaagtagtt tccaatgtga
    39781 caactctagg aaaatggcaa cgtcaaatca tgcaggcaaa actggcaatc aaaaaaatcg
    39841 ctacctcgcc agaaacatgg tttgggctag agtacagcga agtaaaacat gaaactgttc
    39901 cgcttatcat tggaaatact cggaaaacat ggttgaaaga gcggttgtga aagtagcgct
    39961 ttgacctgaa ctaatgtcga atgtcagcta cttgaacacc gtttatcaga ggtcatgaaa
    40021 aagctgtact gcaacaagca gtaagaacga gcagataaaa acagcttctc atgtttttca
    40081 tctgtttggg cttaatgccg tagatgcaag cttttgaata ccgtttttat cagaggtcat
    40141 gaaaaagctg tttagcaaca agtaacgcaa tgattttgaa gtgataagga gatgtggctg
    40201 aaaaatgccg catctccttt tttattccta aatatgaata cgcattattt tcaatctttt
    40261 tgtagaaaaa gggtacaata gaaaaagaga aaggcgggag aatatggtta tcaatttcat
    40321 tattttattt ttgatgattg caattacttc attttttgta gcaagtgaat ttgctttagt
    40381 gaaaatcaga cgatcaagat tggaacaact agaaaaggaa aatgtaaaga acgccaagct
    40441 agctcttcat gttacacacc atttagataa ctatttatca gctagtcaat taggaattac
    40501 gctaaccggt ttgatcatcg ggtgggtcgg tgaaggatct gtcgcggctt tactagaacc
    40561 agtgatcggt aaactacctt ttagttcggc tattagcagt acaatctctg tggcgctggg
    40621 atttatttta gttacgtata tcgatgtggt tattggcgaa ctgcttccta agagctatag
    40681 tatcgtcaat acggagaagg tcgtcttgtt cgtagttaaa ccacttcatt atttttataa
    40741 agtaatgttt ccttttattt gggtattgaa tcattcagct gcagggctag ggaagctatt
    40801 aggtgtgcgt ctcgtatctg aaggagaaga gacattgtct caagaagagc tgacattagt
    40861 cgcattgaat tcttatgaaa aaggagagtt gaccaaagaa gaatattatt atttggaaaa
    40921 cgtgtttgaa tttgatgata ccttggcaaa agatatacaa gtagaccgta cgtccatgca
    40981 agtattcgaa gcttcagaaa ccgtcaaaga agcgataaaa gcttcgattg aacaaggaca
    41041 tactcgctat ccggttatct tagaatcaaa agacaatgtt ttaggttatg tcaccttgcc
    41101 agatttgatc aagcaatctt ataaagatga ccagctgaca gtagaacagt tgattgaaga
    41161 gccgatcgta acaactgaaa cgatccctat aaaaaaatta ttgacgatca tgcgaaaaaa
    41221 gggtaagcat attgctatct taaaagatga atacggaggg acaagcggct tagtgacgat
    41281 tgaagatatt ttagaagaaa tcgtcgggga aatccgagat gagacagatt tggacgaagc
    41341 attgatagcg gaacagtcag atggctctta tatcatctcc ggcaaactaa cattagatga
    41401 tttccagcgt tattttcatg tagagattcc agagtttgaa gaaacgaatt ttactacact
    41461 tgcgggcttt gcttctagcc gatataaaga aataaaagca ggaacaatca ttgagattgc
    41521 atcattccgt ttcacggtat tagaatacca gcatgcacat atcgattatt tcaaagtaga
    41581 gtccacggaa agaaaaacag aataaaagaa aaaaggctgt gacatttttt gtcacagcct
    41641 ttttgttatc taaaaaacct gttttcggta aacgaagaag atattttcaa taaaagtttt
    41701 catttttagc cgcaacgatt tcttttaatc aaggctatct tgcttctgca tgttctaatg
    41761 tgaaatgttc ctcttcaccc atgtgtttca attgtttcat cgaacgcata taagagtaaa
    41821 gcaacacggc aacagaaccg atccaagcta aaggagaagc tgctgctgct cctgggtatc
    41881 cccacattcc tgtaagaacg atagctgcaa aagaacgcat aagcaattcc atcataccgg
    41941 cgatcgtagg gatcttgctt tgtccaagcc cttgaagtgt gtagcgtaaa atgaacagga
    42001 ctgctaagat ccagtaaaga gacccattga tattgaagta ggtttgtgct aattcaaata
    42061 cacgtgtttc tgatgggcta acaaacaatt gaacaaagaa atgaccgaag aaaatgacga
    42121 tagcacctgc taagagacta aatccgacac tcatcagtaa agtttgacgt accccttgca
    42181 agattcggcc atattctttc gcaccatagt tttgtgcagt gaaagttgcc attgcaatac
    42241 caaatgacat catcggctga tttgcaaatt ggtcgatacg accagcagcg gcttgagcag
    42301 ctacgacatc tgtccctaga ctatttagtg ctgcttgtaa cacgatagca ccaacagcga
    42361 tgatcgaaga ttgaaacgcc attggcaaag cagcatttag gtgtacagca aactcttctt
    42421 tttcaaattt gaaatctttt ttgcgtaact gtaacaaagg catttttttc ttgatataca
    42481 ctagacacag tacgctggaa gaaacttgtg cgattactgt agcaatagct gcaccttcca
    42541 cacccatgcc aaaatagata ataaagatca aatcaagtac gacgttgatg atgaccgcaa
    42601 tgatcagaaa gaatagtggt gtgcggctgt caccaagtgc gcgaatgaca ttagaaagca
    42661 aattgaaact gactgatgca aagatcccta ataaaataat tgaaataaat gtttgtgctt
    42721 gatcaagtat ttcaggagga gtttgcatca attgaagcat cggacgaata aacaggagac
    42781 ttaggacagt taaaatcaca gtgacgacaa gactaatgac aacactagca gcaaaacttt
    42841 tcttcagacc ccgataatct tttgcaccgt aacgttgtgc ggtaataatg gctaaaccag
    42901 ctgttaaacc ttgggcaaag ccaatgatca aaaatgtcag gcttccagtt gcaccaaccg
    42961 ctgccagcgc atttttacct agcgtctgac ccacgatgat catatcgatc atattgtaga
    43021 actgctgaaa aacatttccg atcagtaaag gaacagtgaa catcaaaatc agtttagcgg
    43081 ggcttccttt tgttaaatcg cgcatctttt aaactcctta tatctttaaa atttagttag
    43141 tagtatatag cgtttatcct gagaatacca attagaaatc aatgataatt tatgaaactc
    43201 cataaaaaga agccaagaat tgagtcaaag aatcaacaac ttggcctggt ctgtaaaata
    43261 agggacatcc acgtttatct atcaccttaa tattgtctaa tctcacttgg taaaaggatc
    43321 gtttctctcg taccgtcatc ataggcgtat acttgagcag gaaattggtt cgaataggca
    43381 aacggcaaat caatatccat ttccacattg cttcctgctt gagaaaaatg catcgtcact
    43441 tttccacgat tttcgatcaa atcaaaggtt aatatatttt ctaatggaat gactccctta
    43501 agatctaggt caataataaa ccatatactg tcaatcaatt catctggcaa acttgatacg
    43561 acaccaactg aagcgtaacg gttgcggctg ctatcaaaac tttcaaacat ctgtttaacc
    43621 tccttcgaat caacgaatgt tttattttta taattaatcc taggataaac tagaaatact
    43681 tacttcaacg agggttcatt ctaataaaga atcaggaaaa atgcaacaat aattgaatac
    43741 tttttttcaa a
//
LOCUS       DASOUD010000009        43371 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.009, whole genome shotgun sequence.
ACCESSION   DASOUD010000009 DASOUD010000000
VERSION     DASOUD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.009"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(57..2138)
                     /gene="cadA"
                     /locus_tag="U4A29_000509"
     CDS             complement(57..2138)
                     /gene="cadA"
                     /locus_tag="U4A29_000509"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEO0679658.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(2662..3021)
                     /locus_tag="U4A29_000510"
     CDS             complement(2662..3021)
                     /locus_tag="U4A29_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEO0679659.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3051..3392)
                     /locus_tag="U4A29_000511"
     CDS             complement(3051..3392)
                     /locus_tag="U4A29_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679660.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(3389..4063)
                     /locus_tag="U4A29_000512"
     CDS             complement(3389..4063)
                     /locus_tag="U4A29_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO0679661.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5116..6414)
                     /gene="asnS"
                     /locus_tag="U4A29_000513"
     CDS             complement(5116..6414)
                     /gene="asnS"
                     /locus_tag="U4A29_000513"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO0679662.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6454..7644)
                     /locus_tag="U4A29_000514"
     CDS             complement(6454..7644)
                     /locus_tag="U4A29_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO0679663.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7665..8192)
                     /locus_tag="U4A29_000515"
     CDS             complement(7665..8192)
                     /locus_tag="U4A29_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEO0679664.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8239..11028)
                     /locus_tag="U4A29_000516"
     CDS             complement(8239..11028)
                     /locus_tag="U4A29_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO0679665.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11175..11375)
                     /locus_tag="U4A29_000517"
     CDS             complement(11175..11375)
                     /locus_tag="U4A29_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO0679666.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11828..12148)
                     /locus_tag="U4A29_000518"
     CDS             complement(11828..12148)
                     /locus_tag="U4A29_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679667.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12651..13769)
                     /locus_tag="U4A29_000519"
     CDS             complement(12651..13769)
                     /locus_tag="U4A29_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679668.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14240..15547
                     /locus_tag="U4A29_000520"
     CDS             14240..15547
                     /locus_tag="U4A29_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO0679669.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15668..16867
                     /locus_tag="U4A29_000521"
     CDS             15668..16867
                     /locus_tag="U4A29_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEO0679670.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(16894..18162)
                     /locus_tag="U4A29_000522"
     CDS             complement(16894..18162)
                     /locus_tag="U4A29_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO0679671.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLFYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPILQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(18326..18973)
                     /locus_tag="U4A29_000523"
     CDS             complement(18326..18973)
                     /locus_tag="U4A29_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEO0679672.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(19382..19885)
                     /locus_tag="U4A29_000524"
     CDS             complement(19382..19885)
                     /locus_tag="U4A29_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO0679673.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(20008..20772)
                     /locus_tag="U4A29_000525"
     CDS             complement(20008..20772)
                     /locus_tag="U4A29_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO0679674.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            20880..21155
                     /locus_tag="U4A29_000526"
     CDS             20880..21155
                     /locus_tag="U4A29_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO0679675.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            21820..22770
                     /gene="yidC"
                     /locus_tag="U4A29_000527"
     CDS             21820..22770
                     /gene="yidC"
                     /locus_tag="U4A29_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO0679676.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            22874..24190
                     /locus_tag="U4A29_000528"
                     /pseudo
     CDS             22874..24190
                     /locus_tag="U4A29_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(24891..25091)
                     /locus_tag="U4A29_000529"
     CDS             complement(24891..25091)
                     /locus_tag="U4A29_000529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679677.1"
                     /translation="MAEMHDHLLHSICERTGLSEEEVNQKLMEISEAMNKGVDFSMNQ
                     LDDFLAQHMKDALASDAADSVE"
     gene            complement(25441..25788)
                     /locus_tag="U4A29_000530"
     CDS             complement(25441..25788)
                     /locus_tag="U4A29_000530"
                     /inference="COORDINATES: protein motif:HMM:NF019886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO0679678.1"
                     /translation="MDHLHDHDAENRLESNYARYLGGIEQTIEDSYTQDCMMSYTPDI
                     VEKIAPTDFKKMERKRLQEEIQKLTFNQRKVIEHYYFLNKNQQQIARDLRMSHAEITK
                     TLKRAFAVMKEVY"
     gene            complement(26183..26560)
                     /locus_tag="U4A29_000531"
     CDS             complement(26183..26560)
                     /locus_tag="U4A29_000531"
                     /inference="COORDINATES: protein motif:HMM:NF025104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679679.1"
                     /translation="MFFLAFLYLYIANVKYFSKWPLLLFWIISCVLLLVKNAVFQFLL
                     KKIGSKSHTRVLVVENGNLAKEYGLAMLSDPDADANIIGYVKDRKKEDILLEEPGFAN
                     YGDGLAKLKYFGRISDVQDVIVK"
     gene            complement(26584..26907)
                     /locus_tag="U4A29_000532"
     CDS             complement(26584..26907)
                     /locus_tag="U4A29_000532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679680.1"
                     /translation="MIDRRDDSDRCWSIRYYYKGRINEIIKERFWNTVFDCIGDAASI
                     FVAYLLAMFICYHVLEAAPGINMLSVPYLMMAALYSILIADFLRIFRATNKTYFRKYQ
                     NGFLK"
     gene            complement(26969..27445)
                     /locus_tag="U4A29_000533"
     CDS             complement(26969..27445)
                     /locus_tag="U4A29_000533"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679681.1"
                     /translation="MSQIKFLVMDVDGTLTDGKIYMGECGEIMKAFNIKDGCAIHDIV
                     RAHNIEPIIITGRRSKILENRCKELGINILYQGIDNKIEKLLEITSDLSTVAYIGDDI
                     NDLSCMHPVKSAGGIVACPHDAVKKVLEISDFIAEHDGGNGAVREFVEWLVDTQNN"
     gene            complement(27462..27827)
                     /locus_tag="U4A29_000534"
     CDS             complement(27462..27827)
                     /locus_tag="U4A29_000534"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO0679682.1"
                     /translation="MELEEGRYDLSNGIYLNVESYDIYRFEERMYESHLKYLDIQCIL
                     LGCEYIVVESISNLQIEVDYSADKDIVFYSNSIVGNCVLLEGGKLLLLTPEDAHMPCI
                     RVNESAKVKKAVFKISINY"
     gene            complement(27910..28875)
                     /locus_tag="U4A29_000535"
     CDS             complement(27910..28875)
                     /locus_tag="U4A29_000535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEO0679683.1"
                     /translation="MDKIIEAKRVFDIEIEALKRTRDCLGKTFVRILDAIVNCEGKVI
                     ITGMGKPGHIAAKLAATFASLGTPAFRLHPAEAMHGDLGMVSEKDIVIAISYSGESDE
                     IVKILPNIKMIGATLIGITGNSKSTLATASDIVQILPEFEEACYLGLAPTSSATSVLC
                     YGDALAVVASGIYGFKDSDFGKFHPAGSLGKKLILKVSDLMSHGNEIPMVNSGTLLMD
                     AISVMSNKGLGIVSVIDQDGTLKGIITDGDLRRIIERKVDIYSVEIDDVMIANPKVFT
                     KDKLAVDALRFLKANSINILPIIDDEGMLVGTITWQQIVKAGIVA"
     gene            complement(28984..29760)
                     /gene="kdsA"
                     /locus_tag="U4A29_000536"
     CDS             complement(28984..29760)
                     /gene="kdsA"
                     /locus_tag="U4A29_000536"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010388312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEO0679684.1"
                     /translation="MNRLMLIAGPCVIESEENVMLIAKTIKEITEKLDIDYYFKASFD
                     KANRTSITSYRGPGIEEGLKILKKVKETYGLKICTDIHEPWQAEKAAEVCDILQIPAF
                     LCRQTDLLVAAAKTGKIINVKKAQFLAPWDMKNVINKLVEAGNSNIMLCERGTSFGYN
                     TLVVDMTSIVEMKKFGYPVVMDATHSVQKPGGKGDVTGGNRNNVEPLAKAAVATGADA
                     LFFEVHPDPDNALSDGPNMVLLDEFEGLLERVLKVYNAVR"
     gene            complement(29762..30499)
                     /gene="kdsB"
                     /locus_tag="U4A29_000537"
     CDS             complement(29762..30499)
                     /gene="kdsB"
                     /locus_tag="U4A29_000537"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEO0679685.1"
                     /translation="MKTIAIIPSRYGSSRFPGKPLAMISSKPMIQWVYENVSKAAMLD
                     AVYVATDDKRIFDCVESFGGMALMTSDKHTCGTDRLAECAEILQLRDEDLVLNIQGDE
                     PLIKAEMIADLYSCFDNDEVYMGTLKKHIEVQEELDNPNVVKVINDVNDYAIYFSRFC
                     VPYERDGKNSLHFKHIGVYGYKTWFLKKYSTMPKTPLEVSESLEQLRVLESGFKIRVK
                     ETKYQTVGVDTPEQIQQVEDELRKEGL"
     gene            complement(30496..31923)
                     /locus_tag="U4A29_000538"
     CDS             complement(30496..31923)
                     /locus_tag="U4A29_000538"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024832.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEO0679686.1"
                     /translation="MNRLIKNYAYNLCYQILVMILPIITTPYVSRVLKAEGVGEFNYS
                     YAIVSVAVIIAQLGTNMYGQREIAYVQNDKIRRSGVFWSIFVIRIISSFIVFPVYILI
                     SATLHSYTVLLISMSVYLVANLFDVSWFFQGMEDFKKTAIRSMVVKLIGLVLIFLLVK
                     NENDVVLYSVILAGTELLGNALLCCHIPKYVRRISIHDISITPHIRPILGLFLPTAAV
                     YIYTYVNKIILGIFSTDVQVGLYSQPEKIVKLLMTVITSLGAVMLPHIAVAVKENNID
                     RIRKEVLNAVSFVFCIGAPMVVGAIIIANTFIPWFLGDGYAKSVSLFQLLSPLILIIG
                     FASVVGQAVLIPMKRQRVYTFSIMTGAVINLVVNLALIPHLEAIGAAIGTIFAELTVT
                     AIQFYEVRNCIELKMASIITNVKNYLIGALIMGGVGGYVSSKLPNSVISTFITVILCI
                     LLYSILLICMKDKLLMDYLKRRKNR"
     gene            complement(31925..33238)
                     /locus_tag="U4A29_000539"
     CDS             complement(31925..33238)
                     /locus_tag="U4A29_000539"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEO0679687.1"
                     /translation="MIYQLLIVEFILILIMYILSNRCFISPAFIAIALFFLATCCVVY
                     NIEFWNVQYSKKTLWFITTGFLAMCIPEMYILFLDYLSKKRVDNYHQNQYKIEYVQIY
                     PSRQLNLIFALLSVVFFGVLLVEILRKGDYSTLGLYAIGAAKSDETQNSVIARFGISL
                     ITGLNLAYLYVFINNVIRCKERVLSNIKYLFPTLMYILCMFISGNRVFLVKLFAIVFL
                     IYIVMKSDVSPRGRVPVMQVVRKTLFVAVAILAVFYALRGITKVNSTAATREFSDYIT
                     YYIGSPVYMLDKYLQRYSPELNWGAKSLTSLVEMFGIEPEFKNAYIHVGGDSNFAGNE
                     FSWFQRPYSDFGWLGMLVFTFIVFWIFSWNIHIRINKEKNRRRRKYYILITAYFFYIP
                     VMSFYYCQMCFAFSITNIFSVVVACLLVREFTKRQDEIIFKIKEE"
     gene            complement(33239..34384)
                     /locus_tag="U4A29_000540"
     CDS             complement(33239..34384)
                     /locus_tag="U4A29_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HEO0679688.1"
                     /translation="MKKARVLAFYLPQFHPVRENDIMWGKGFTEWTNVAKAKPLFKNH
                     YQPQIPADLGFYDLRISEVRKQQAEMAKKYGVEGFCYWHYWFGNGKRILELPFNEVLQ
                     SGEPDFPFCLGWANHSWSNKTWQKSKHFTKDVVFLEQTYPGDEDYILHFKSILPALKD
                     DRYIKVDGKPLFLVFAPDSIPDSAHFISLWNKLAKENGFEGIYFVARVTSVDNKFDLS
                     SMTDVAVHNRFQRFLDMGYDAINSDMQRYAELKISGYWKRGIKSIMRKTVGNFSANLY
                     PYDKIMENFYCEEEKRECVHPQLIPRLDRTPRSGKDARVYVDSTPEKFGNAIDRALSY
                     VQDKEPEHRILFLQAWNEWGEGNYMEPDLVFGCEYLEILRKKILGEE"
     gene            complement(34381..35187)
                     /locus_tag="U4A29_000541"
     CDS             complement(34381..35187)
                     /locus_tag="U4A29_000541"
                     /inference="COORDINATES: protein motif:HMM:NF014075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HEO0679689.1"
                     /translation="MVNIKIRKVIHRIPPILKLMRFVVDVQNKYFYDKFCKNTDNIQR
                     IEKLRNKFLGETCFIVGNGPSLTMEDLELIKDKDCFAANLIFKIFDKTQWRPKYYFIQ
                     DRYADTGNELDDIDLEYMFIGDYYWRVRGMHNPNAICIHSEQSVGGKNIKFSEDISKS
                     IVSHETVTYSMIQAAVYMGYKKIYLLGMDHSYALTYNADGTVSEDKTIRSHFFEDKNA
                     NEVIASIVGMNQAYIRAREFCESNNIKIYNATRGGKLNWFDRITLEEAVK"
     gene            complement(35246..35434)
                     /locus_tag="U4A29_000542"
     CDS             complement(35246..35434)
                     /locus_tag="U4A29_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016396235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO0679690.1"
                     /translation="MVVKGTSDDFYHANDEGGLAWNDPEIGIEWPQVLGEYKGNASAE
                     GYIFEDDTPLNISEKGQR"
     gene            complement(35782..35916)
                     /locus_tag="U4A29_000543"
     CDS             complement(35782..35916)
                     /locus_tag="U4A29_000543"
                     /inference="COORDINATES: protein motif:HMM:NF013621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEO0679691.1"
                     /translation="MKEKVIEYTTGVYDMFHIGHLNVMRNAKAQCDFLIVGVSTDEPV
                     "
     gene            complement(36257..37390)
                     /locus_tag="U4A29_000544"
     CDS             complement(36257..37390)
                     /locus_tag="U4A29_000544"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO0679692.1"
                     /translation="MRQKIRVLHIAQAAGGVDCYIRMLLKYLDKEKFENILICSQDFC
                     ETDYANLVLSFKQIKLTREIDLNDLKAIVIIRKLIKKYNPDIVYAHSSKAGAITRVAK
                     IGLKNYCVYNPHGWAFNMRCSVKKKMLYTAIEKIAALFCDKIICISDAEKQSALKKRI
                     CCEDKLQVIFNGVDIEAYRNKAHGEVKRESLNIPQDAFVVGMVGRISPQKAPEIFIKM
                     ARLVKNKIPEAHFIIVGNGEQKALIEKYAAEHGFLESLHITGWVNNSISYIELFDVAC
                     LLSRWEGFGLALPEYMMVGKPIIATNIDAIPNLISNGKNGLLVPVDDETAACEAVFKI
                     FSDKAMAARLTSEATSVVLEKYNSKRVASEHAVLFEMLVKKRG"
     gene            complement(37302..37854)
                     /locus_tag="U4A29_000545"
                     /pseudo
     CDS             complement(37302..37854)
                     /locus_tag="U4A29_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            complement(38323..39012)
                     /locus_tag="U4A29_000546"
     CDS             complement(38323..39012)
                     /locus_tag="U4A29_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO0679693.1"
                     /translation="MESEDLLSRISKVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYAAYRIRKEEPGSPIFFSHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTQIGKIIRAKSIDELPQLWNVLKGDMSLVGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSGRNDVSFHAMVDLDLEYITNQSLLNDFKLIIKT
                     IWIVIKPNGAY"
     gene            complement(39066..39830)
                     /locus_tag="U4A29_000547"
     CDS             complement(39066..39830)
                     /locus_tag="U4A29_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO0679694.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIVMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIQRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(39858..40556)
                     /locus_tag="U4A29_000548"
     CDS             complement(39858..40556)
                     /locus_tag="U4A29_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEO0679695.1"
                     /translation="MARTQKQKATPTHAVSLITLTNPSSPIAEQYRTIRTNIQFASTT
                     GQQIKTIVVTSSGPGEGKSTTASNIAVVFAKSGQRVLLVDADLRKPVIYKTFQLNNAS
                     GLSTALSSSGSVADVIQRAPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPIVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDDGYYYGN"
     gene            complement(40569..41348)
                     /locus_tag="U4A29_000549"
     CDS             complement(40569..41348)
                     /locus_tag="U4A29_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEO0679696.1"
                     /translation="MEETISLEEIFETLRKHLITIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVQERLKTEDNVKMTP
                     EQIKDAISINQSENSQMFSIQAISTSAMTAQQIANTTSQVFQENAKDVMNVDKISIVS
                     GAVANTTPISPNNKLNLVIGLMLGMMVGVVLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSEKELKAGIRKKGLAPTRPKVTNDKTASSRRSRSRV"
     gene            complement(41364..42308)
                     /locus_tag="U4A29_000550"
     CDS             complement(41364..42308)
                     /locus_tag="U4A29_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO0679697.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLNKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGVQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSILNALESNMKTDMSFDEMKKIALDYREAFDTIKQD
                     QMQGEGFMQDDISYQKVNDNELDRVKKELKEQMNLENK"
     gene            complement(42345..43121)
                     /locus_tag="U4A29_000551"
     CDS             complement(42345..43121)
                     /locus_tag="U4A29_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679698.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNIS"
BASE COUNT        12843 a         9299 c         7163 g        14066 t
ORIGIN      
        1 tttcattaag aaaaggatat aaaaaaaaga ggacgttcat tcgtcctctt cattgatcaa
       61 cgtttatttt ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca
      121 acatctgcaa atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc
      181 aaaattttta tcccaatagc aaagacaatg ttctgtttga cgattttcaa tgttttcttt
      241 gctaaatgga ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct
      301 gcttcaattg cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct
      361 agcaccggtg catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt
      421 aagatttcct ctaaccgctg cactttatct tctggaagca attcgctata tatcttgtcc
      481 acgcctactt gcttcccgat atgatcagca atttttttcg aatcgcccgt caacatgact
      541 gtgttcttca caccagcctt ttttaattga accaatgcgt ctacggcatc ttctttcaag
      601 gtatctgcaa taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta
      661 ccgatttctt gacttgaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct
      721 gctaatactt gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcttggata
      781 ttagctgttg gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt
      841 gtagaaaact gttctgcact cgcaacatat tgcatgaata tctttggatc gatttttgta
      901 tcgatcgtct ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact
      961 gtctcagttt gtgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta
     1021 cttgctcctc cgataccgcc aaagaaacta aggggtacgg agatcactaa tgcacatgga
     1081 catgagatca ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa
     1141 acaacaagtg gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa
     1201 tagcgcgcga atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct
     1261 actaattcta ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa
     1321 aatggtttat ttttattgat aaatccactt aatactgttt caccaacata tacagattta
     1381 gggactgatt ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt
     1441 ccatctaacg gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct
     1501 tcagggttca cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc
     1561 gccatcaatt cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg
     1621 ccgatttgat aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg
     1681 ccaatcgtag caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc
     1741 atcactgcct tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc
     1801 aagcgaattg gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat
     1861 aaaatccgga acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta
     1921 tctatttctg ttactttgac atctggttct aattccttca ctactttttt tatttcttgt
     1981 tcaatcgtag ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt
     2041 accgttgctt ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag
     2101 ttcgcacaat ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa
     2161 catatgaata tctcttcata actaattata aatgaacggt tattcatatg tcaaggtatc
     2221 cgaaagaaaa aatgaaaaac atctgcatcg gaaaatttct tccatacaga tgttttggct
     2281 tattctttct ttttcttttc gatcattgcc actgctctta tccaacccgc acttgccttc
     2341 ttgatcttga caggatagca actaagtttg aagcctgttg ctggcaattt gtctaaattc
     2401 attaattttt cgataggaca atatcttttt tcaatccctg caaaatgtcc ttcccagata
     2461 ttcgaagctc cactacgctt tcaaaaatat agaaagagtt ctttaatgaa aattatttca
     2521 actttccata aacagtaacc ccatataaat aaaagacgta aaagtcacat ggaagaactt
     2581 atacaggaga acgcatagag tcaggtgaaa tacatgtatc ccacctgact ctatgcttta
     2641 tctaaccttt attattctct tttatacaat ttctgcaccg aatggcatca acgccaatcg
     2701 agcaagtttg aaagattgct tggcaaaagg tatccctatg atcgtgatac atagaaggat
     2761 gccgctgacg acatgtgcga gagcaattgg taggcctgaa acgatcagcc agatgatatt
     2821 aacaatcaat gacatgccag actggtgata aattacttct ttgccaaaag gcgcaaaaga
     2881 aagggaggcg atcttaaaac attgcatacc gattgggata cctatgatcg tgatacacca
     2941 aagtatcccc gctgctaacc agctaagacc accgattagt ccgccgaata taaaccagat
     3001 gacatttcct aaacaactca ttgtatttct ccttctatta ctgtttttga ttaaaactgg
     3061 gcaaaaaatg attttattct tcttttttta gcttccagta tcaaatattc tgcaaatttt
     3121 ttttggattt cttccgtatt ttcttttgtt actaatgtgt gttggattag ttttcctgat
     3181 ggtcgctgat ccaatcgttc ttctgactgt tgcacgtccg cttgcgcaaa tccgataatc
     3241 cgtttttctt ttgttacagc aacgagcaat agtttattct tatcatttag gtcagctgcc
     3301 gtttttgctt ttacgaatga ttctggatga tcagtaatca gctgattgat ttgaggtaga
     3361 tcttctttta ctgcccaccg tatttccatc atttctcact tttctttcgc tcatttttat
     3421 ctccactaaa aaaatcgact gcggcaattg catctatcca gctttgtgca atgatctcat
     3481 gtccgccaat cgttgggtgg accccatctt ctcttgtata tttcgaaaaa ccataaacaa
     3541 tccctaaagc attgaatata ccatctaaag gaaccagcaa tgcgccatat tccttagcaa
     3601 gtttacgaac aatttggatt ttagggtcta agtctttacg ccagccatga cggtcgtcgg
     3661 ttataggaag tacaaacggc tccatcaaga tgatgttgca gattcccact gtttgaagtt
     3721 catttagtaa ttttcgataa acggattcaa attgctgagc agatttctca gttccgaata
     3781 tagcctgatc gctagtatta tgccaggtat cattgattcc gatcaaaatc gatacatagt
     3841 caggttgtaa agctaaacaa tcttcttgcc agcgtacttg cagatctgca attttgtctc
     3901 ctcctatccc acggttcatg cattcaaatt ccatcgttgg atattgggag attagttttc
     3961 cggcaatcaa aagtggataa cctttaccta gatcagttgc ttctgctcgg tctcttcctg
     4021 cgtctgtaat gctatcaccg ataaataata tacgtttttt catttgtcaa aaactccttt
     4081 ttgaataaaa cgatgatttt cttccttata ttatcccata agccctatca agacgaaaga
     4141 gaaaatatat gtataaaaaa tgacaccagc ctagtccctt caaacaattg aatgaacgct
     4201 catcaagtgt ctagtttttt atgctatttt tttcttaacg tgccggattc cccttgattc
     4261 cttcgttgtt tggtccagag agatcgatca cacctaagct tccgtcaatc agaccactga
     4321 tgacgccatc agaacatata tttataggct catctaagac ttctagtaaa tgtcccttta
     4381 ctaatatctc ttctagattg cttgggatag attggtcata actttatctg gtccgatgat
     4441 ttcaagggtg aattgaactt acatatggat tattgcatgc tgaaaatacg gacctactcc
     4501 atcttcgaat gttactatta gtttgttttc aggtgctaga tgctgcttta tttcaacagc
     4561 agaagcatca tcaaatcaga tttccatttg aattaccttc tttgtaaatg tttgattgat
     4621 tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt tcttttgctt tcttctcttg
     4681 tctgctggaa acttccttta tcccgtattt taaaaaaaga actgcgagag ttcgcagttc
     4741 ttttttagaa taaacggtgc tctccggcca actcctcggt cacaagtcac aatagtcatc
     4801 aaacatgaaa aactgttttt tgaaattact ccttgtgact cggagctgaa cggcaggcgc
     4861 acaacctctt ccttaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg
     4921 caaaaatgtg gaaagccatt tccacaaaat ttttcttaat actcgaaact aaacagcttt
     4981 ttcacaacct ctccactaaa cggtattcaa aagcttacat ctgcggtaat aagtcccgcc
     5041 aattcaaaaa catgaacagc tgtttttgct tggctgttct aattacttga tgcagaacag
     5101 ctttttcatc acctcttatg gataaattcg gttcaacaaa cgtgggaatg gaatagcttc
     5161 acgtacatgg tcgataccag caatccaagt gactgttcgt tcaagaccta gaccaaaacc
     5221 agagtgcgga acagaaccgt atttacgtag atcaagatac catgaatatt ctttttcatc
     5281 caaaccgtga ttacggattt gttctagcaa gaaatcatag tctgttgccc gttcgcttcc
     5341 gccaataatt tcaccgtaac cttctggtgc gatcatatct gcacagatca ccacgtcttc
     5401 tcttgttgga tgtggtttca tgtagaatgg cttgatggct tttggatagt ttaggataaa
     5461 gacaggctgt tcgaatgaat tcgcaatgaa cgtttcatga ggtgaaccaa agtcttcgcc
     5521 ccattcgatg tcatcaaatc catttttctt cagtaattcg atcgcatcat cataagtaat
     5581 acgagggaat ggcaattttg tataagtttt taagacttct ttgtctcgtc ctagtacatc
     5641 taatgcatat tcgcaatgat ctaaaacatt ttgtactaag aatgctacat attgttcctg
     5701 tacttctaaa ctttcttctt gatgagtgaa agccatttct ggttcgatca tccagaattc
     5761 gatcaaatga cgtcgtgttt ttgatttttc agcacggaaa gtcggaccga atgaaaagac
     5821 ttttccgaaa gccatagcag cagcttccat gtataattgg ccactttgag ataaataagc
     5881 tttttgatca aagtaatttg tttcgaatag atccgtagtg ccttctggtg cagaaccagt
     5941 caagatcggc ggatcaattt tgacaaaatg atgttgatta aagaattcat acgttgcacg
     6001 aatgatttca tttctgatct gcataatcgc atgttgtctt gaagaacgca gccataaatg
     6061 acgatgatcc atcaagaagt ctgttccgtg ttcttttggt gtgatcggat aatcgtggct
     6121 ctcgcttacc acttccacat ttgtaattcc gatttcataa ccaaattttg aacgactgtc
     6181 ttcacggatt tctccagtta ctaacacgga tgtttcttgg tgtagatttt tagctgtttg
     6241 gaaaacttct tccgacactt cgctttttac aactacacct tggaaaaagg ctgttccgtc
     6301 tcttaattgt aaaaatgcga tttttccgct tgatcgctta tttgcgaccc aagcgccgat
     6361 tgttactgtt tctcccacat ggtttttcga atcgatgatt tgaatttgtt ccacgtatga
     6421 ctctcctttt atattccttg aatttcttta ggattaattt tgacttttac tctcaataaa
     6481 ttgcttgatt ctgcgtaccg cttcttctaa cgtagcgcga tcagtagcat aactcatgcg
     6541 gacattttct ggtgccccaa agccttcgcc agtcacaagc gctacccctg tttcttctaa
     6601 aagatcttct acccattgtg tgacattttc atatttacac atgcgtagcg tttcttttac
     6661 attaggaaaa agataaaaag ctccttgtgg cttttcaaga gaaacacccg gcagttctgc
     6721 cacaagcgaa tataacgtat tcaggcgttc ttcaaaggcg ttccgcattt cttccactgt
     6781 atcttgctcg cctttcaatg cttcaacagc tgcatattga ctgactgctg tcggattact
     6841 ggttgactgg gaagcaacag cgatcattcc attgatgatc acaggatttc ccaccgcata
     6901 accaatgcgc cagcctgtca tcgcatatgt tttagacacg ccgttgatga tgatagtctg
     6961 atttttgatt gcttctgaaa tcgttgcaat tggagtaaac tcattgccat tataaactaa
     7021 acgaccataa atatcatcag caactatcaa aatatcattt tttactgccc attcaccgat
     7081 tgcctgcagt tcattcttgc tataaatcat tccagtcgga ttcgatggag aattgatgat
     7141 tacggctttt gttttttctg aacgtgcttg ttctagctgt tcaacagtaa ctttccagct
     7201 attggattct tcccctttca caaagacagg aagtccttct gccagtttta cttgctcacc
     7261 gtaactgacc cagtaaggca caggaatgat gacttcatct tgcgggtcaa ggatcgtttg
     7321 gaaaagggta tacaaagcaa atttcgctcc gtctgttacg actgtttgtg aaggttcata
     7381 gacaagtcca tagtttttct tcagataatc aacgattgcc tgtcgaagct ctgggattcc
     7441 tgctgttggt gtataatagc tagctttccc attgcggatt gcttcgattg ctgcttcttg
     7501 gatgttttct ggtgtcgcaa aatccggttc tcccacagtc aaactcagca catctctgcc
     7561 ttttgctttt aatgccttgg cctttgccgc agctgccaaa gtgacagacg gttctaatcg
     7621 ttttgttctt tttgataact ccatcccatt cattccttta ccttttaaat tcccttgatt
     7681 gtcttgactt catctccaga ttcaaagcca atcaggtaat aattcagttt cccatttgtt
     7741 tcttttccta ctacttccca cacaggctga tcatcataca tccctaaagc agctttttcg
     7801 atttggattt caggatgggc cgcagcaatt gtctgttttg cttcgttttc agtcagtccc
     7861 tttgtttgat ccaatacttt tactttttca ccagatttag gaatgataac ggcgatttct
     7921 tttcctttat catttgtacc tgtcagtgaa aagtatgtct tctttcgagt aaaccaataa
     7981 aaatgatcgg tttcctcaag atttgcatat ttctttgcga tttctactgc ttctttcttc
     8041 gcctgcaccg ccggacgact ggaacgcaag taaaaaatcg agctgatcac gatgaccgct
     8101 aataaaaata cgatcaagcc aagtaacacg cgattaatcg ttttttcctt tgtctgtttt
     8161 ttcttcaagg gtcgtcgctc ctttatcttc attccttttc attatagcag attttaatta
     8221 tgaaaaaaga cagctcctct aacttccttc ttcatttttc tttaagaatt catggatatc
     8281 ctgatagatt tcctccatag ctagctcttt gatcggtact tttttcggta atgcatggat
     8341 gatccgtgcg ccatattttg cagtgactag tcttcgatcc aaaataagca atacgccacg
     8401 atctgtctcc gtccttatca agcgacctaa tgcttggcgt aacttcaaag ctgctttagg
     8461 tacggattcc tggtaaaaag gattgatccc ctctgattct aaataagcgt tcctagcttt
     8521 gaccatcggg cgaagcggat tatcgaaggg taaacgtgtg actacaacga tctgcaaagc
     8581 atctccaggc aaatctacac cttcccaaaa gctgtcagcg ccaaacaata cgcttttttc
     8641 tgacaaaatg aagcgtttca aaagcttctc ccggcttcca ccaaaccctt gagctagtat
     8701 ttctcgtcct ttttctagca tagacagatg cattttctga tagaccttct gcaaaacatc
     8761 atgagaagtg aataacacaa gtattggccg atcttcttgt tcgatcagac ctgtaagtgc
     8821 tgtctctaaa taatcgatat atgcttgatt gctgacttga tggacggcag gtgcctcttt
     8881 tggcacatag atccttgcct gtttctcata atcataagga gaggcaatca ctttaagagg
     8941 tgctgcttca atgccaagcc tcttagagag atagtgtcga ttagggccga tcttcagcgt
     9001 tcctccaata taaagaatac gatcatatcg aggataccaa acagtcgacg gcaacatagc
     9061 cgcttgaaaa tcatggagat gaaaaatcat ctgctgcgct gtgttcccag gaacgaacca
     9121 atggatcaaa tgatctcgcc agtcttccag ccattgatcc atcagcaagt attgcttgtc
     9181 taattgatca aataggctat ataaacttgc taaatcgatc cgcatctggt ttgtccagct
     9241 ttcttggata atctcaaaag tcagacgcat ttgtgactgt agctgctgga tctcctgata
     9301 aaaaagctgc aggtgatcaa tggctgtccc agattccagt ggcagctgat ccaacatctc
     9361 ttttgtcatc atgacttctt ctggatactg tccgttaggt tgcttgccgc tcactaaact
     9421 cattaatcct tctgccatac aaatctgtgc ttcgcatagc gcagaaagtt cttctcgata
     9481 aatcgataac cagcgtaagg cttcatgttc tttcggtaaa tagctttgta ttcggtcaaa
     9541 cagctgattt ggttcttcta attgctggat ttgtttatgg aaatagttaa tacttaatcg
     9601 tttgtcattt actttttcag cgatttccgg caaatgatgt acttcatcga tcaacaaaaa
     9661 tggactagct ggaagcaatg gttcttttcg ctgtgtttct tgtactaaaa acgcatgatt
     9721 gacaatcaaa aaattacttt gcctgatttt cttttgcaag aaacgaacaa agtcttgttc
     9781 gtaaaatgac tgatttcttg ctaaaaatcc aatacctctg tgcgcgatat ctgcaaagaa
     9841 tgaatggttc aaatttgtta aatgtaattc atccaaatca ccagtcttcg tttgcgtcag
     9901 ccaaactaag accgccattt gatacaaagc atattgtttt tgttctggcg gttccacaag
     9961 tgtgccttta aaacgctgca aatcaatata gtggcgatag cttttgataa ttacaggttg
    10021 tatgggctga tctagcaatt gattcagcaa tggaatgtct ttttctatga tctgctgctg
    10081 caaaactagc gaaacggtac tgatgacagc gggtttttca ggtgtagcca aataactcaa
    10141 tggcagcaga taaccgattg ttttacccat tcctgtagct gcttcgatca tcaaatcttt
    10201 tgattcatct tttgtgaaat ggtcgtagac cgcattcatc agtctgttct gttcttttcg
    10261 atacatcagt gttttaccaa acattttttc ttttgcttgt ttttttcttg gatatgtccg
    10321 ctcgccataa tggacggatg tgaatagttc cacttctttt ttctgcaaag ctagcccatc
    10381 gacgatttct aatgagggat caagtggctc tggtttttct ttcatttctt caatcacatg
    10441 ataaatgaaa cgactcgtat ccatacctgt gtgtctactt aatcgcgcaa tctgctctaa
    10501 cgtgatgatt ggcaattttc tcatccgttc ttcgatcagc aaaagaagtt gacctgtcac
    10561 ttcggcatca ctatctgcct gatgaggatt ctcatgaata aatccaaggc tttctgacaa
    10621 atcagctaag cgaaagcttg gttccgtcgg caagaaaatt tgtgcaagtt ctactgtatc
    10681 gatgcctggt atcttcaaac taggaagtcc gcatcgcatg agttcatggt tcaaaaaatt
    10741 ataatcaaaa taaatattat gcgcgacaaa tatggtgtct gccaaaagat tgtaaatcgt
    10801 ttgggcgaca tcttcgaaat aaggcgcttt ttggacgcgt tgattcgtga tccctgttaa
    10861 atgctggatt tgttttgaaa tcttccgccc tggatgaata tcgatcgaaa aacgtgaagt
    10921 gatccgacct gattcaatca tgacgcagcc aaattggatg atccgatctt cttttggatt
    10981 cgtcccagtc gtttcgatat caacgactgc ataagttctt ggtttcaaaa tatcttcctc
    11041 ttctcctgcc tctaatgttc ttttgaaatt atactacaaa gtgcagaaaa atactctcat
    11101 cgctttttaa agaaaataga caaaaacgcc cagacacgca aatgtctggg cgtttatttt
    11161 ttagtatagg ttaattaagc tttagtaacg tttgttgctt gaggtccgcg ttggccttct
    11221 tcaacatcga aagttactgc ttggccttct tctaaagttt tgaatccgtc gccttggata
    11281 gctgagaaat gaacgaatac atcgtttccg ttttctgcag tgatgaatcc aaatcctttt
    11341 tctgagttaa accattttac tgtacctgtt tccataataa aaatcctcct tgtgtatcaa
    11401 cacatatttt gcaattactt gaatggtgta tatctggaag atgtttattt gaaacaagct
    11461 accacagatt accgaacaaa aactactcct atagtatatc ataccgttta caaaaaagct
    11521 agagtttttc gacttttttt attaaaaaaa gttttctcag cgattacata aagtgaaaaa
    11581 ccgaactttt ttttaaaata aacgacatat tgttcagttg gtgttttttt gctctgatgt
    11641 tcttttttaa actaaaaaaa ttattctttc tgactactta tcggttctgt ttcaacggtc
    11701 gaggctgggg aagattcttt agtaggtctg atgttctcat ggtatttctg atagcctatg
    11761 acagttacca gaagaacaag gcaaaccaat acgaccaacg agattagttt ttcttttttg
    11821 ctcatatcta tacctcataa ataacaggat gatgtccatc atatttctga gtatgagtaa
    11881 aagcaaaagc tgcagctgta gaatgtaaga aatgccagca actttcataa tcacaggtcg
    11941 gacagtacca aaagtaacca aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg
    12001 cagaatcaag atctggtgtc tgtacataaa catttttgta cagacgtgtg cctgtaatag
    12061 gaaaccgctg tgtttcttca gcaaaaactg gctgacttgt taaacttagg gatagagaca
    12121 gcaaacacca taacagtctt tttttcataa agacctcctt atcgataagt ttttcccaat
    12181 taaaatcata tctcaagaac gcccgttatt cacattttgt tcataaatga tgttttttgt
    12241 atcataatcg tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc
    12301 agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa taatttttgt
    12361 caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaaacggtg
    12421 ttcaaaagct tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa gagctatttt
    12481 tgcttgctgt tcttattact tgatgcagaa cagctttttc acaacctctc atacacggtg
    12541 tttaatcagc tgaccttcgg tattagctca aaaaacggca aaatatgtga aactattttt
    12601 ccgtttgttt ctctaatact caggtcaaaa cactgatttc acaacctctt ttaataacga
    12661 agaatcgctg tcagactctt ttcatctggc gcatcttttt gatccaaaat aaacactttt
    12721 ccgccattaa tgatcacatc atctgctaat gcattcaaca cttgacgccg atcatattct
    12781 gtttcaggat cttcaccgaa accatcagct aagtttgacg tagcgataaa taaatgtgac
    12841 acttttcctt ctttcgcagc tggaacgata tcaactaact gatcgatgaa tttcttatct
    12901 aagagtttat tgtattcttt tgattcttga gctgcgagtt cacttgacag cttcttcgcc
    12961 ccttcttcga tatctctaaa ggatagctgc gcaggagaag cagaaatcgc aacgtctgca
    13021 caataatagg gatttttagc aatttttttg aacatcgtct gattttctgg tagagcaaat
    13081 aaataaatcg gccattgttc tggattttcg aaagaatcct tcaagtacgt atcaactgct
    13141 tggtaatagt tgacccaatc gatctctact tcttcatctt ttggatttac gccatggaaa
    13201 gttactccat ctcgggctcc gcctcctcca ttagaagaga agttcaaacc gccacctgtc
    13261 agttcagatc ccaatgcagt aacgacatca accggtgccc cttctggtag agatacttct
    13321 tttacttgtt tgttgctgac ttcatacaat ttcatggagt cgcggttcaa tcccaataaa
    13381 taataagaat agttgaactg cccatttttg ataatagcca gtaaataagg aagatcactt
    13441 acatagtatt gatcgtctac cgggatactc aaacgatgga tataggtttg gtcagcagtt
    13501 ataatgaccg ctacactcgt agttgcacta cgccaaaacg aaggatctcc taacaaattt
    13561 tcaatttttt cttggaacgg ggaccagtca tgttcggaat attttttctc aaaacgtttc
    13621 tttgcttctt tcgcaaaatt tttgaatttg atctgatctt tttccacatc ttgatgggcg
    13681 acatgcgtat tcagcatgat cgtgacgaat ggtcctttag cgccttctga agtcaattgt
    13741 gacaataatt ctttatcctt ttttgccata aacgtacccc tcctaaaaaa atcattcctt
    13801 tgtttctatc taaagtctac cacaacacta tttttccata caataaaacg tatctaaaaa
    13861 aataaaacgg tttctacggt tttaaattta gctatttttt taagttatct tcataatttc
    13921 ctcaattatt tagccccagt cttcaactat tcttcatgaa atgctgataa actaagaaaa
    13981 agaataaaag aggtggatct tatgcatttg attatgattt tcagtttatt aagtattatc
    14041 agtttatttg ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc tattcctgta
    14101 aaagtacgta aaaacgaccg ctaaccttcc ataacgtgcg ttcttatcct aaagtttttt
    14161 cagaaacatt gaaactctct cccctctctg ataaaatttt aatttgtagc gaaacaaatt
    14221 aattacaagg agatcggata tgtccataaa aaaaacacgt ctagtcctat tattggctag
    14281 tctcattctt tgccttgctt cttttctacc agctgttgct gtgcaagctg aagatacttt
    14341 caaagtaaat gcaaaagcag cctttgctgt agatgcgcaa agcggtaaga ttctttatga
    14401 tcaagatgga gaaaaaacca tggggatcgc ttcaatcaca aaaattatcg gtctttacat
    14461 cgtccttgat caagtaaaag aagggaaact atcatgggat gataaagttt caatttcaga
    14521 ttatgcagaa aatttgagtg tcaccccaga tttatcaaat gttcctttgc ataaagaaaa
    14581 cacctacacc gttaaagaat tattcgactc tgcaatcatc cagtcagcca acgcttcaat
    14641 ggttgccttg gcagaaaaaa tctctggcag cgaagcaaaa ttcactgaac gaatgaaaga
    14701 acaattgaaa gattggggta tcaaagatgc aacgatcgtt aatgcatcag gactgaataa
    14761 ctcttatctt ggcgaaaacc gaccagaagg tactggagaa aacgacgaaa atcaaatgtc
    14821 tgctcaagat gtggctatcg ttgcccgtca tttgatcttg gattttccag aaatactgga
    14881 tgtatctagc actacgacac aaatgtttgg cgaaaacacg caatcaccag tagaaatggt
    14941 caactggaac tggatgctcc caggattcgt gaattataaa gaaggcgtgg acggattaaa
    15001 aactggtacg actgaacttg caggagcctg tttcgtcggt acgatcacta aaaacgatca
    15061 acgaatcatc actgtcgtat tgaatgcaga cggccatgcg gaaaacccaa gtgcccgttt
    15121 caatgaaaca gctcgcttaa tggattattg ctacgacaat tggtcagaaa aagagcttgg
    15181 aaaagccaat gcaagtatcc ctagtctaaa aaccattgat gtaaaagacg gaaaagaatc
    15241 aacagtgccc gttgtgctta aaagtcctgt aaaagtatgg gtacggaacg atatggatac
    15301 tggaaaatta acaatcacac caactcttga caaaaaacaa gtcactgaca atgagatcca
    15361 agcacctatg gataaaggaa caaaaatcgg taccgcaacg atcaccttgg cagaagataa
    15421 attaggttat ctagaagaca acaacgcgcc atccacagaa atcatcacgg ataaaaccgt
    15481 agaaaaagca aatattttcg ttattggctg gcgacgcgtc gcagaatttt tcggtaactt
    15541 gttttaattg aacaaaaaga aaaaagcatc tttccatgtg gaagatgttt ttttgctttt
    15601 tctagtttaa ataacagctt ttttcatcta ttttcgttat actgtataag aaaaaggagt
    15661 tgaatttatg aatctaacca gagatctgat ttttttggtt ctctatctta ttggggtatt
    15721 ctccatcgtt tattggcgaa aacagaaaaa ggatccttcc ttgttctttt acagcagtac
    15781 gtggacattt gcggtaatct tgacttattt tgtcgtgttg gaaatttttt attcgacctt
    15841 ttattttttg attcccttag tgtttttcag tatctttgcc ttttcctatt tttcagaaaa
    15901 gcgaaggctg ctgaatggtc ttttattcaa tgttttcctc attagttttg gtatctactt
    15961 gtttgtcttg ctatacgaaa cacaaaatat ttttcttggc ggactgattg ccttgatcac
    16021 gatcccgtta ctgcttgtct ttctattcgg catttatgga ttgatcgttt ttcttttttg
    16081 gaacggtgtc acagttttac gtagagagtc gcattcatta gctaatcttt taacgttgat
    16141 actagccatt tttcttacat tgtttcttgt ttttgacttt ttcttgttaa aatatttacc
    16201 acaatggata aatgctttgt ttttctgcat cccattgatc ttgatctatc tatttatcgt
    16261 attctacaac tttttgactg tctcattctt gtatcagttc aaccgccctc gatacaacca
    16321 agacttcatc gttgtgttag gcgccgggtt gatcaatgga gaaacagttt ctcctcttct
    16381 tgccaagcga atcaataagg cgattgcttt ttattgggca caaagccgtg cgacattaaa
    16441 tccgcctatc ctattaatgt cgggtggaca gggagcagac gaaaaagtac cagaagcaat
    16501 ggcgatgaaa caatatgcca tggaacaagg aattcctgaa agagatattc ttgtcgaaac
    16561 caattcgaca accactttgg agaatatgcg ctattcaaag gaaatcatgg atcagcaaat
    16621 gaaagggccc tatcgcgcga tcttcagttc caacaattac cacattttca gagctggtct
    16681 ctatgcacga caagcgaaac tgaaggcaaa tggcattggg gcaaaaacag ctttctatta
    16741 cttgcctaac gcgtttttaa gagaatacat cgccatcttg atgatgaaca aaaaaagaca
    16801 tatgatcatt tgcggattgc ttttcgcagg ttccgcattc ttatcactga tcactttact
    16861 tttctaaaaa accaaggtgt gacaaaacat atgtcacacc ttgttttttt cttttattct
    16921 cttgacgatt ggcaagaata gtattccttt tttgacggct acttgctgtg cagaataaat
    16981 accgctgttt cctgaacaca taaagctgat caagcagatc ataaagaagt aaacagcagc
    17041 ttgtgaacca aataattcga ttcccatcac aaaacaggca atcggtgtat ttgtcgcacc
    17101 agaaaatacc ccgataaaac ctaatccagc aagaaaagga acagaaacat ggagcaaagg
    17161 cgctagtact gcgccaagcg ttgcaccaat ctcaaatagt ggtgtcactt ctcccccttg
    17221 gtaccccgca cctaaagaca agaccgtgaa gaataatttc ccaataaaat cataagcatg
    17281 tgcctgtcca ttaaatgatt cctgcagtat cggaagactc aacccaaggt aacgttgtgt
    17341 ttgcaaaatc aacgctgcgg ctacaactac tgctccacct atcgtatttc gccaaacagg
    17401 atgaggaatc cattgcgcat accgcgcttt taaaaagacg atcgaacggc tgaataccca
    17461 accaatcaaa ccaaaagcaa tcccagcaac aaaaactttg aagaacagca acatactcca
    17521 ttcaggaatc acacccattt ggtaatgtag atgagaaaca ccatagcttt ctgtcatgaa
    17581 atttgcaaaa agcgcagcaa agaagctagg aaagattgct tctgttcgga ctttaccgat
    17641 tgccaacact tctaatccaa ataatgttcc agctaaaggt gtgccaaata cagaactaaa
    17701 acccgcactg attccactga taaccaaaat ctcccgttct gctttatcca atcggaataa
    17761 atgcgctata ttatcagcta atgctccccc catttgaact gctgttcctt cacgaccaac
    17821 agatccccca aataaatggg tagtgatggt cccaaacaag gtcaatggaa tgagccgcaa
    17881 agggatcttc tcttctcctc cattcccttg atcgatcact aagttattcc cacgactaga
    17941 aagaccgcca tgatacgcat aaagataagc aaaaagcgcg ccgctgactg gcaatagata
    18001 aaatagccaa ggatgtgcta gtcggatatc tgtcacgaac gtcaggctat ttaagaaaaa
    18061 agcagacaag ctccccatga atactccgag aatcgctgtg accaccagcc acttcaacat
    18121 gtacatcaaa atacgcactg gactcggtaa ttgtttttcc ataaaaaatt cctcctcaaa
    18181 taaaaaaacg cctactaaag aaaggggctg gccccagact ttagtaggcg tcattagcta
    18241 tcttacgtta gcatttaaag gcgaacacca tcacctattc acttccatta aaagatagca
    18301 tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca gcaaattcat
    18361 agaaaggagc caatttcgtt aactgttgat tacattgatc actatcatga caaatataat
    18421 tccccttttt cgtatacgtg ccatcagaac cggctttcgt tgtagaaaga aagagcgaga
    18481 cattcgatgt tttctgacag atcgcacaga ttcccttatt aatcgttggt gataaagtac
    18541 cgctgatccc ttttaatttt ccatcttcgt aataaaccat gaattttttt tgcgtccctg
    18601 gatcattcca accgatgtac gtatgtgctt gcaagtcgat ttcattccaa gcgggttgtt
    18661 tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctcggc tgcataaacg
    18721 gctggacgat ttgcttcagc tcctccaatg ctcgttctgc ttgcgttttt gtcagtttta
    18781 catctaaaac tgtcgttaaa aacgtatcga tttctgatgg ttcgtaacct aatacatcgc
    18841 gaatcttttc agctgctaga gcctgaaccg tttgtaccgt ttttgagtca ttgacagatg
    18901 agtacgcatt taataactgc tctacttctc tttttataaa aaaatattga tatggtttca
    18961 ttgtttgttc catgatttta aagtcctttc ttctaaccaa agtgcatact ttgtagaaaa
    19021 aagtgatctt ctgcaaagta tgtgttacct gaagatcccg gactctttta aataagatac
    19081 gttttgcatc ttatcatcac cccctttaat gttcattctt ctatgtacat gattcattcg
    19141 ataaataaaa gtaaaccgta tcttgagcag atagtcaact gcaaacagaa aaaggactgt
    19201 gacaaaagtc gtgtcaaagt ccctttctac aaacaaacgg tgttccagaa gtagcttctt
    19261 caaaaaataa gccggaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
    19321 tttcttgaag ctgacccact tctgtcacaa cctcaatatg gtctattatt ggttttacct
    19381 gttaaatcga ggattctttt ccgaagtaac gttgccatct cttacgcata cttgctgaca
    19441 taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg atatatcctt
    19501 ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg atatctcgct
    19561 caagatccga caaatcggtg atcttttctg cattctttac agcgattctt ggatggatat
    19621 acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac agatcatgca
    19681 aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg agcagataac
    19741 tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc tgtgtgtaat
    19801 tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac atatattctt
    19861 cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga atttcatttt
    19921 cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg ccttggataa
    19981 aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa caatccctgc
    20041 cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata cgttctgatc
    20101 agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga ttattgcgaa
    20161 gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt ctgtgccata
    20221 cactgtaaac cctttgtctt tcattaaagg aatcacttct gtcaaatctt tttgcaacac
    20281 aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat aaatatctgc
    20341 cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac ggatcatcgt
    20401 ccctacattt ccaggatctt gtacattatc taataacagc catcctcctg aaaaatctgg
    20461 cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag gtagatccga
    20521 tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt ttgcggccag
    20581 ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta tttctgcatc
    20641 agacgacgca gcttcttcga tcagatggaa cccttccaaa agatattctt ccttttcttc
    20701 ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt ttgctgattg
    20761 gatctctttc acattcctca cctccccatg attatatcat ggaataggtg cttgagcttg
    20821 tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag gtgacgaaca
    20881 tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt cgctacatga
    20941 ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa gacgacggct
    21001 cagtcacaat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt gaagaagtca
    21061 agaaatcacc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa gacccgatga
    21121 ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct ggtctgcatc
    21181 aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg ctggacttaa
    21241 tgccgtagat gcaagctttt gaacaccgtc aataaggttg tgagaaagtc gcttagtttt
    21301 gagtagtaag gagaattttg tgaaaatggc tttctacatt ttttcataaa atttgactta
    21361 ataccgagaa actgactttt gaacaccgtc gataaggttg tttttgaggc gctttgcctc
    21421 gagtattaag gagaaacatt ttcaaaatag cttctcatat tttgttaata ccgagaaact
    21481 gacttttgaa caccgtcgat aaggttattc ttgaggcgct ttgcctcaaa aattaaggac
    21541 agcagtgaaa aagctgatct atcacttgca gtgagttcat ttgactccgc tttttcgggc
    21601 agaaaaaaag tttttagttt attaaagtaa gaaagaaaca cttggaatca ttctactttt
    21661 tcctagtatt tctttcttga ttttttgtct ttttttcaaa atttttcgct acgtaaaaga
    21721 acgaatattt atatgaaatt tctatgaagt tgattgaatt ccatgcgctt ttttagtata
    21781 gttaagtttg tgtaaaaatt atagaagagg aagtattcta tgaataaatg gaaaaactgg
    21841 ttattgggat ccggacttgt cagtatcctg ctctttttat ctggctgtgt gcggatggat
    21901 tcaaacggaa accctgacac ttcgggaatc gtttatcgag tactcgtcca tcctatgggc
    21961 caagcgatta catatctagt tgaaaatttc aattggtctt atggctgggc agttattgca
    22021 atgactgtga tcgtccgtat catcatcttg ccactaggta ttagtcaatc gaaaaaaaca
    22081 atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag ttgaagcagc ccaacaaaaa
    22141 ttgaaagctg caacaacacg tgaagaacaa atggcagcac aagcagaaat gcagcaagta
    22201 taccgcgaaa acggattgag tatgaccggc ggaatcggct gtctgcctct attgatccaa
    22261 atgccgatct tctctgcctt gtactttacg gcacgttata cagaaggtat tcgggaatca
    22321 tctttctacg gcatcgattt aggctcacca agtatggtac ttgttgtcat tgcaggggtc
    22381 gcttatctgc ttcaaggata catctcaacg atcggtattc ctgaagaaca gaaaaagacg
    22441 atgcgtacga tgctgatcgt ctctcctgct atgatcgtct tcatgtcgat cagtgcgcca
    22501 gccggtgtta cactttactg ggtagtcggt ggtgtgttca gctgtctgca aacattcatc
    22561 acaaacgtta tgatgaaacc acgtttgaaa gcacaagtgg cagaagagtt gaaacaaaac
    22621 ccaccaaaac aagtcgtcac tccaagaaaa gatgtgacag aatcagctaa accagctgac
    22681 agcaaacaag caaccaaaca aatcaaacaa ccaaaaaaca gtacaaacaa caaaggccgc
    22741 aacgcaggaa agcagcagaa gcggaaataa aaatatatga caaaaaactg tagacaaata
    22801 aaaaaatttc tttgttctac agtttttttg ttacaatcaa agaaaacgtt cgcttttcga
    22861 ggagggatgt ataatgatgt tcaaacaatc aatggatagt cgaatggaag aagacaaaat
    22921 ttcgctagat gcacttgttc ctaaaaatca tttagtccgt aaaattgaca agttattgtc
    22981 atttgatttt atttatctca ttgttgaatc aacttattcg accatcggtc gacctagcgt
    23041 tgatcctgtc gtcttagtaa aattagtatt cattcaatat ttatttggga ttcgttcgat
    23101 gcgtcagacc attaaataag tttaaacaaa cctagcttat cgttggtttc ttgggcttag
    23161 tttaattgat aaagttcctc atttttcaac ttttggtaaa aactatgcac ggcgtttcag
    23221 agaaaccgat ttgtttgaac agatatttat cattattctg gaacaagcag ttgttgctag
    23281 atttattcat gaagaaattt tgtatatgga ttcaactcat attaaagaga atgcgaaaaa
    23341 acgtattttt acaaaagata tggcacataa agaagccatt ttataccaaa aacgattgga
    23401 agaagaaatc aatgaggcaa gaattgccga aggcaaacgc cattttggtt caatcattcg
    23461 tcaggaggtg gtcgaaagga aatttagcgt cgcagatcca gaaattggtt attatgtaaa
    23521 aacagaacga gaaaaacagt ttgcttattc ttctcatact gtttgtgatg aaaatggttt
    23581 tgtattagat gtcatgatta cgcctagaaa tctacattac agccgaatgc tcgtgccaca
    23641 aatcgaacgt gttaaatcac gttatggtgt agctgctgat gctgcgtaca aaacaccttg
    23701 gaacgccaag tatttaatcg accgaaaact acgacccact tttccttata ctcgacccaa
    23761 aggaaacaaa gaacgtttta aaaagaaaga ttttgattat gatccctatt ataactggta
    23821 cctatgcccg aatgatcaaa cacttcagtt tcgcacaact actcgagatg gctataaaaa
    23881 atatgtatct aaatctttta tttgtgaaag ttgtccatta ttgaaaaatt atacagaaag
    23941 ccagaaacac caaaaagaaa tacatcgaca tatttgtgaa gattacttag atgaagcaga
    24001 acatttacgc catacggtgt acaatcgcga aaaatataaa aaaagaaacg attgaacgca
    24061 tttttgcaga cgcaaaggaa aagcatggag tgcgctacac caagtatcga ggcttggaaa
    24121 aaatcaggtg catacgatgc ttgtttttgt cgcaatgaat ctgaaaaaat tagccacttg
    24181 gcagtgggag catatgtctt tatatctttt attatacaaa aatagacaaa agtatcagtc
    24241 tacactctta gtcaagagga aactgatgct tttatctaca gtctgagaga gtgggacaat
    24301 agtcctttct ccaaaagaaa acaagtccga ctttgatagt taattttaaa atcggacttg
    24361 ttttttcttc tttacgtctt ttcctctcca tgtatttccc taattttctt aaatttaacg
    24421 ccatgagtgc caagccaatg ttaatcttgg ctccttgttt ccctcgtaag tggaaatccg
    24481 tttccgatag atcgaacctt ctttttcctc taaaaacttt ttttacattc tgctttgaaa
    24541 taacgccatg tattattcac taaaatttgt cggtttcgct cactttttgc tttcgtacat
    24601 tgactacgaa cagggcagca ccaccataag ccgcctgcca gttacctctc actcttaccg
    24661 ggtctttcag aacacccgga tgctctaaca catcactcgg ctaggctccg ttattgaaga
    24721 agaaagcccc atatctttcc atggcatcct cggccagctt aagaagctcc tccatctgtt
    24781 cggagacctt cctcacatat atgggcaaag gccatgaaga cctccaccag actgttcatg
    24841 accttcttca gtgctgcgat catctgctct ctcttctcca tggcttacac ctactcgaca
    24901 gaatcagcgg catcagaggc aagagcatcc ttcatgtgct gtgccaggaa gtcatccagc
    24961 tggttcatag aaaaatcgac acccttgttc atggcctcgc tgatttccat cagcttctgg
    25021 ttcacctctt cctcagacag gccggtgcgc tcacagatgg aatggagcag atgatcgtgc
    25081 atctctgcca tcttctggtc catgtcgaat ttttcgtggc agcaatctct gcgccatgcc
    25141 ttcggaaata cggcttcccc ccctcatcag actgtgtgtt tggttccagc tctttcttcc
    25201 gggattcttc gctctcggaa atcggtctgt acagtgaaca cacataaata ttcttcctat
    25261 gactggtatc actcatggct tgtctccttt atttgttttg tcgagtccta cctcaaagga
    25321 ggtctggcca gcatataggt gttcatctga ctgtcccttt caaaattagt gtcctcatct
    25381 atgatttcgg gcgttttata actcctccgg acaaaatttt tgaaaaaatt tgtcgagggc
    25441 ttaatacacc tctttcataa ctgcaaacgc acgcttcaag gtcttggtga tttctgcatg
    25501 cgacatgcgc agatccctcg caatctgctg ctggttcttg ttcaggaagt aataatgctc
    25561 gatgaccttt ctctgattaa aggttagctt ctggatctct tcttgcagac gcttacgctc
    25621 catcttctta aaatcagtcg gagcaatctt ctccacaatg tcaggagtat aggacatcat
    25681 gcagtcctgt gtgtagctgt cctcgatggt ctgctctatg cctccgaggt atcttgcata
    25741 gttggattcc agacggttct ctgcatcatg gtcatgtaga tggtccactg atagtccata
    25801 tactcctcaa ccaggatgaa gctattctta tcggcgaacc gatacaacat tccattcatg
    25861 cggtcagctg tgaagatgga gtacatatca ttgcgaatat agaggataaa gtcgttgcga
    25921 tacacggtca acagagaatc gtccatctag aactgagcca cactcacttg gcaccatatt
    25981 atcactcagc taacggaagc cgggctgctt tacgcctttg gtgctgtcct tgatttcctt
    26041 ccatatactg cttgccgaga ttgatgattc ttctgacatc gatcaaagta acaggtaatt
    26101 tagaatcttc cacaaacctg attatctcct ctactacttt cggctcaata tcttctaatg
    26161 cgatcacgat tttatatacc tgctacttca caataacatc ctgaacatca gatattcgtc
    26221 caaaatactt taatttcgcc aatccatccc cataattagc aaatcctggt tcttcaagta
    26281 gaatatcctc tttctttcga tctttcacat atccaataat atttgcatct gcgtctggat
    26341 ctgataacat ggccaaacca tattctttag ccaggtttcc attttccaca accaaaactc
    26401 gagtatggga cttgctgcct atctttttca gtaaaaactg aaatacagca ttcttgacga
    26461 gcaacaaaac gcacgatatg atccaaaata aaagcaatgg ccatttggaa aagtatttca
    26521 cattagcgat atataagtat aagaaagcaa ggaagaacaa acttccaata ccggttaacg
    26581 atactatttt aaaaatccgt tttgatactt tctgaaatag gtcttattcg ttgcacgaaa
    26641 aatcctcaaa aaatctgcaa tcaaaatact atatagcgca gccatcatca aatacggaac
    26701 tgacaacata ttgatcccgg gagcagcttc taaaacgtgg taacaaataa acatggccaa
    26761 tagataggct acaaaaatag atgctgcatc tccaatacag tcaaaaactg tgttccaaaa
    26821 tcgttccttg ataatttcat tgattctccc cttataataa tatcgaatgc tccaacatcg
    26881 gtcagaatca tctcttctgt ctatcattcg ccacgtagaa gttgttggaa aagagtgaca
    26941 accgttcaag acgaacggct atattgtatt aattattctg tgtatcaact aaccactcaa
    27001 caaattcacg cactgcacca ttaccaccat catgttcagc gataaaatct gaaatttcta
    27061 gaaccttttt aactgcatcg tgcggacagg ccacaatacc acctgcactt ttaacaggat
    27121 gcatacaaga taaatcgttt atatcgtctc caatatatgc aacagtactt agatcactag
    27181 taatctcaag cagtttctct attttattgt ctattccctg ataaagaatg ttgataccaa
    27241 gttccttaca tctattttct aatattttgg aacgacgacc agtgataata ataggctcaa
    27301 tattatgggc tcttacaata tcgtgaatag cgcaaccatc tttaatattg aacgccttca
    27361 taatttcccc acactcaccc atatatatct ttccgtctgt tagagtacca tcaacatcca
    27421 taaccagaaa tttaatttgg ctcatacact ttccccccct ttcaataatt gatagaaatc
    27481 ttaaatacag ctttctttac tttagctgat tcattcactc ttatgcaagg catatgagca
    27541 tcctcaggag ttagtagcag cagcttccct ccttctaaga gtacacaatt gccaactatt
    27601 gaattactgt aaaacacaat atccttgtca gctgaataat ctacttcaat ctgtaaatta
    27661 gatatagatt ctactacaat atactcgcat cctaagagaa tacattgaat gtctaaatat
    27721 ttcaagtgag attcgtacat tctttcttca aatctatata tatcatagga ctcaacattc
    27781 agataaattc cattggaaag atcatatcga ccttcttcta attccaacaa ggaatttttt
    27841 ataagaaaat atagacttct ggaaattcaa tttccatttt tttagactta actttttcat
    27901 ttatctcctt tatgcaacaa taccagcttt tacaatttgc tgccatgtaa tggtaccaac
    27961 aagcatacct tcatcatcga ttataggaag aatgttaata ctattagcct ttaaaaatct
    28021 caatgcatcg acagctaatt tatctttagt aaatactttg ggattagcaa tcatcacatc
    28081 atcaatttct acagaataga tatctacttt tctctcaata attcgtctta aatctccatc
    28141 agtaataatg cctttgagag ttccatcctg atcaatgact gaaactatac ccaacccttt
    28201 attcgacata acagaaatag catccattaa caatgttccc gaattgacca ttggaatctc
    28261 attgccgtgg gacattagat cagacacttt aagtattaat tttttgccca atgaacctgc
    28321 gggatgaaac tttccaaaat cagaatcttt aaatccataa ataccgctag caacaacagc
    28381 taatgcatcg ccgtaacaca acacacttgt agcactagaa gttggagcca atcccaaata
    28441 gcaagcttcc tcaaactccg gtaatatctg gacaatatca gatgcggtag caagcgtaga
    28501 ttttgaattg cctgttattc caattaaagt agccccaatc attttaatat taggaagaat
    28561 tttaacaatt tcatcggatt cgccgctata gctgatagca attacaatat ccttttctga
    28621 aaccatacct aaatctccat gcatcgcttc agcaggatga agtctgaatg ctggtgttcc
    28681 taagcttgca aatgtcgctg ccagcttagc agcgatatga cctggctttc ccataccagt
    28741 gataatgact tttccttcgc aattaacaat tgcatcaaga atacgaacga aagtcttccc
    28801 aagacaatct ctcgttcttt ttaaggcctc aatttcaata tcaaaaactc ttttggcctc
    28861 tattatctta tccattttat ttacctcttc agtttttaat agcagctgga ggtctttcac
    28921 atgttgtaac acagtacaaa atgtgaaaga cttaccatac atttctatgc aatgcaagaa
    28981 aaattatcta acggcattgt atacttttag tacacgctct aataacccct caaattcatc
    29041 caagagaacc atattcggac catcagacaa ggcattgtca ggatcaggat gtacttcaaa
    29101 gaacaaagca tctgcgcctg ttgcaacagc cgccttcgca agaggctcaa cgttatttct
    29161 attacctcct gtaacatctc cttttccacc aggcttctga acactatgcg tagcatccat
    29221 aacaacaggg taaccaaact tcttcatctc aacaatagat gtcatgtcaa caaccaatgt
    29281 attatatcca aaagaagtgc cacgttcgca aagcataatg ttactattac ctgcctccac
    29341 aagtttatta ataacatttt tcatatccca cggtgcgaga aactgtgctt tttttacgtt
    29401 aattattttg cctgtttttg ctgctgccac tagtaaatca gtctgtctac acaggaatgc
    29461 aggaatctga agtatatcgc atacctctgc agccttttct gcttgccaag gttcatggat
    29521 atctgtacaa attttcaatc cataagtttc cttaactttc ttaaggattt tcaaaccctc
    29581 ttcaatacca ggacctctgt aagaggtaat actagttctg ttagctttgt cgaaagaagc
    29641 cttaaaataa taatcaatat cgagcttctc cgtaatctcc ttaatagtct tagctattaa
    29701 cattacattt tcttcggact caataacgca tggtcctgca attaacataa gtctattcat
    29761 cttatagacc ttcctttctt aattcatctt ctacctgctg tatctgctcc ggcgtatcca
    29821 cccccacggt ttgatattta gtttccttta ctcgaatctt aaatccactc tccaaaactc
    29881 gaagttgttc gagagattca gatacttcaa gaggcgtttt gggcattgta ctgtatttct
    29941 tcaaaaacca tgttttatac ccataaacac caatatgttt aaaatgcaga gaattttttc
    30001 catctctttc gtatggaaca caaaaacgtg aaaaatatat cgcataatca ttgacgtcat
    30061 tgattacttt aacaacattt ggattgtcaa gttcctcctg aacttcaata tgttttttca
    30121 aggttcccat ataaacttca tcgttatcaa agcaagaata caaatctgct atcatctctg
    30181 ccttgataag gggctcgtct ccttggatat taagaactaa gtcttcatcc cggagttgaa
    30241 ggatctctgc gcactcagca agacgatctg tgccgcaagt atgtttatca gaagtcatca
    30301 aagccattcc accaaagctt tcaacacagt caaaaattct cttatcgtct gttgcaacat
    30361 atactgcatc tagcatagct gccttagaaa cattttcata aacccactga atcatgggtt
    30421 tactagaaat catagcaagc ggcttccctg gaaacctact tgatccatat cgagatggaa
    30481 taatagctat tgtcttcatc ggttctttct ccttttaaga taatccatta acaacttatc
    30541 tttcatacat attagtaaaa tactatatag taaaatacac aaaattactg taatgaacgt
    30601 tgatattact gaatttggga gcttactact gacatatcct ccgacgcctc ccataatcag
    30661 tgcacctatc aaatagtttt ttacattcgt aataattgat gccattttta attctataca
    30721 gtttcttact tcataaaact gaattgcagt aactgtaagt tcagcaaata ttgtacctat
    30781 tgcagcgcct atagcttcca aatgtggtat taaagccaaa ttgacaacga gattaatcac
    30841 agcgcccgtc ataatagaga aggtatacac cctttgtctt ttcatgggaa tcaaaacagc
    30901 ttgaccaacc acactagcaa atccaattat caaaatcaat ggtgatagta actgaaataa
    30961 ggaaactgac ttagcgtatc cgtctccaag aaaccacggt ataaaagtat ttgcgattat
    31021 aatcgcacca actaccattg gggcgcctat gcaaaacaca aatgatactg cattcaatac
    31081 ttcttttcta attctatcaa tattgttttc tttaaccgct actgcgatat gtggcaacat
    31141 aactgcacca agagatgtaa tgactgtcat caacagcttc actatttttt caggctgcga
    31201 gtatagccca acttggacat ccgtagaaaa tattcctaat ataatcttat taacatacgt
    31261 atagatatat actgccgctg ttggaagaaa taatcctaat attggtcgaa tatggggtgt
    31321 tatcgatata tcatgaatac ttattcttct aacatatttt ggaatatgac aacataataa
    31381 tgcattcccc aacagctcag ttccagccaa tataactgag tacaatacta catcattttc
    31441 atttttcacc aataagaata tcaaaacaag accaattaac ttaaccacca tacttctgat
    31501 tgcagttttt ttgaaatctt ccatcccttg gaaaaaccaa ctgacatcaa aaagatttgc
    31561 aactaaataa acagacatac ttattagtaa aacagtgtat gaatgaagag tagccgaaat
    31621 aagaatatat acaggaaaaa cgataaacga agaaataata cggataacaa agatgctcca
    31681 aaacactcca cttcttctga tcttatcatt ctgaacataa gcaatctcac gttgtccata
    31741 catgttcgta cctaattgag caattattac cgctaccgat acaatggcgt acgaataatt
    31801 aaactcccca actccctccg ctttcaatac tctagatacg tatggagtcg taataatagg
    31861 cagtatcata accaaaattt ggtaacaaag attataggca taatttttga tcaacctatt
    31921 catattactc ctcctttatc ttaaatataa tttcatcctg ccttttcgta aattctcgta
    31981 ctaaaaggca agcaaccaca acagaaaata tatttgttat agaaaacgcg aaacacatct
    32041 ggcaatagta gaaactcatc actggtatgt aaaagaagta tgccgtaatt agtatatagt
    32101 atttccttct tcttctgttt ttttccttat taatgcgaat atgaatattc caagaaaaaa
    32161 tccaaaaaac aataaaagta aacaccagca tacctaacca accaaaatca gagtaaggtc
    32221 gttgaaacca cgaaaattca ttccccgcaa agttactatc tccaccaaca tggatatatg
    32281 cgtttttaaa ttctggttct ataccaaaca tctctaccaa tgaggtaaga ctttttgccc
    32341 cccaattcaa ttctggagaa tatctctgga gatatttatc cagcatatat actgggcttc
    32401 cgatataata agtaatatag tctgaaaact ctcttgttgc cgcagtcgag ttaacttttg
    32461 taatacctct taatgcataa aatacagcca aaatcgctac tgcaacgaat aaagtttttc
    32521 gcacaacttg cataacagga actcgtccac gaggcgatac atctgacttc attactatgt
    32581 atatcaaaaa tactatagca aaaagcttaa ctaaaaacac acggttaccg gatataaaca
    32641 tacataaaat atacattaat gttggaaaca agtattttat atttgataaa acacgttctt
    32701 tgcatctaat tacattattg ataaatacat acaaatatgc aaggtttaat cctgtaatta
    32761 aactgatgcc aaatcttgca ataactgagt tctgcgtttc atctgactta gctgctccaa
    32821 ttgcatataa tcctaaagtg ctataatcgc cttttcttaa tatttctacc aaaagaacac
    32881 caaagaacac aacagataat agtgcaaaaa ttaaatttaa ttgtctgcta gggtaaattt
    32941 gtacatattc aatcttatat tgattttgat ggtaattatc cactcttttt tttgataaat
    33001 agtctaagaa taatatatac atttctggta tacacatagc taagaacccc gtcgtaataa
    33061 accagagcgt cttcttgcta tattgcacat tccaaaattc gatattataa accacacagc
    33121 atgtcgccaa aaaaaacaat gcaatcgcga taaatgctgg tgatataaaa catcgattag
    33181 acaaaatata cattattaaa attagtataa attcaacaat caataattga taaatcattt
    33241 attcttctcc taatattttc tttcggagaa tctccaaata ttcgcatccg aaaaccaaat
    33301 ctggctccat ataatttcct tctccccact cattccaagc ctgaagaaat agtatccgat
    33361 gctctggttc cttgtcttga acatacgaaa gtgctcggtc tattgcattt ccaaattttt
    33421 ctggagtact atcaacatac actcttgcat ctttaccaga tcgtggggtt cgatctaatc
    33481 ttggaatcaa ctgaggatgt acgcactctc tcttttcttc ttcacaataa aaattctcca
    33541 ttatcttatc atatggatag agatttgctg aaaaattacc aactgtcttt cgcattatgc
    33601 tcttaatacc tcttttccag tatcctgaaa tttttaattc ggcatatctc tgcatatcag
    33661 aatttattgc gtcatatccc atatcaagga aacgctgaaa tctattatgc actgctacat
    33721 ctgtcataga tgacaaatcg aacttattat caacagatgt tactctagcc acaaaataga
    33781 taccttcaaa tccattctcc ttagcaagtt tattccatag tgaaataaaa tgagcagaat
    33841 ctggaataga atctggagca aaaaccaaga ataaaggttt accatcaact tttatatatc
    33901 tatcatcctt caatgccgga agaatagatt taaagtgaag tatatagtct tcatcaccag
    33961 gatatgtttg ttccaaaaac acaacatcct ttgtgaaatg ctttgacttt tgccaagttt
    34021 tatttgacca tgaatgatta gcccaaccga gacaaaaagg aaagtcaggt tctccgctct
    34081 gtaacacctc attaaatgga agttctagaa ttctcttacc attgccaaac caataatgcc
    34141 aatagcaaaa accctcaacc ccatatttct tagccatctc agcctgttgt tttcttactt
    34201 ccgagattct taaatcatag aaacctaaat ctgcaggaat ctggggttga taatgattct
    34261 tgaatagagg tttagcttta gcaacattag tccattcagt aaagcctttt ccccacatta
    34321 tatcgttttc acgaacagga tgaaattgtg gcaaataaaa tgccagtacc cttgcttttt
    34381 tcatttaact gcctcctcta aagtaatacg atcaaaccaa ttcagttttc cgccacgagt
    34441 tgcgttatat atttttatgt tattactttc gcaaaactct cttgctctta tatatgcctg
    34501 attcattcca acaatacttg ctattacttc atttgcattt ttgtcttcaa aaaaatgact
    34561 tctaatggtc ttatcttcag acactgttcc atcagcatta taagtcagtg cataactatg
    34621 atccatacca agaagatata tttttttata tcccatataa acagcagctt gaatcatcga
    34681 ataagtaact gtttcatgcg atacaatgga tttactgata tcctcactaa acttaatatt
    34741 tttgccacca acagattgct ctgagtgaat acatatagca tttggattat gcattcctct
    34801 aactctccaa taataatccc caatgaacat gtattctaag tcaatatcat ctaattcgtt
    34861 accagtatct gcatatctat cctggataaa ataatattta ggtctccact gggttttatc
    34921 aaaaatctta aaaataagat tagcagcaaa acaatcctta tcctttatta attccaaatc
    34981 ctccattgtg agacttggac cattacctac aataaaacag gtctcaccta aaaatttatt
    35041 tctcagtttc tcaattcttt gaatattatc tgtattcttg cagaacttat cgtagaagta
    35101 tttgttttga acatcaacca caaatctcat tagctttaga ataggcggaa ttctatgaat
    35161 cacctttcta atttttatat ttaccatatt tactccttcc gaattacaat atcaaccaaa
    35221 aattcgttat ctaattaaat gtttactatc tctggccttt ctcactaata ttcaatggag
    35281 tgtcatcttc gaagatatac ccttcagctg atgcattacc tttatattca ccaagtacct
    35341 gtggccattc aataccaatc tcaggatcat tccaagccaa gccgccctcg tcatttgcgt
    35401 gatagaaatc atcactagta cctttcacaa cgatattcct ttctttatta aacttttatc
    35461 aactacgata attcaacttt tgatcgagtc ctcatcaatg atactcttaa ttacatgagt
    35521 caactgagtg ctggaaatac catctgtatg gggaagataa acaaattcaa caccggttcc
    35581 cataaactgt tcttcatatt tttcctactg aggattgcct ttctaatcag aacccacgaa
    35641 cattttgtta aatttatatt tctcccatgc cgcatactta tccttatcat tttgagggac
    35701 aaccatatcg acatacttta ttgaatttac aatttcggct cgttctacat acggaataac
    35761 aggtgtctta tttttatctt actaaaccgg ttcatctgta ctaacaccga caatcaaaaa
    35821 atcacattgt gcttttgcat ttcgcataac attcaaatgc ccaatatgaa acatgtcata
    35881 tacacccgtg gtgtattcta taactttttc tttcataaat atctccttta actgtgtatt
    35941 tcagagtaaa ataggaatct ttctcatcaa tctgcatcac actctacatg tccaaataac
    36001 atagaccgaa aagtcaagaa agataatttt ccagtctgtg atgaaccttc tctcttctac
    36061 atacttcaca tagagttcca ttcattcttc gaaagataaa tcatttcgac gcgtaacaat
    36121 cattaccatc gaacaaaaac gttgaaccac atacaacagg cacattcctg cggtaatttc
    36181 tttaatgttt tttctcctga gtcttcactt accccgatat agttaatgcg actcaaaaaa
    36241 tttcttgctt tcatcgttaa cctctctttt taactaacat ttcaaacaat accgcatgtt
    36301 ctgaagctac ccttttggaa ttatactttt caagcactac cgatgtagct tcggaagtta
    36361 gccttgcagc cattgcttta tccgaaaaaa tcttaaaaac tgcttcgcaa gcagccgtct
    36421 catcatcaac gggcaccaac aatccatttt ttccattgct aatcaaattg ggaattgcat
    36481 caatattggt ggcaatgata ggttttccca ccatcatgta ctcaggcaga gccaaaccaa
    36541 atccttccca acggcttaat aaacatgcaa catcaaatag ttcaatatag cttatcgaat
    36601 tatttaccca gcctgtaata tgcagacttt ccaaaaaacc atgttctgct gcatatttct
    36661 cgataagtgc tttttgctct ccattaccaa caataatgaa atgggcctct ggtattttat
    36721 tttttaccaa tctagccatt ttaatgaata tttctggagc cttctgcgga ctaattcttc
    36781 caaccatacc tacaacaaac gcatcttgtg gtatattcaa actttctctt tttacctctc
    36841 catgagcttt atttctatat gcctcaatgt ctacgccatt aaaaatgacc tgcaacttat
    36901 cttcgcaaca aattctcttt ttcagtgctg attgtttctc ggcatctgat atacagataa
    36961 ttttatcgca aaacagagca gctatttttt ctatagcagt gtatagcatt ttcttcttaa
    37021 ctgagcacct catgttgaat gcccatccat gtggattgta tacgcaatag ttcttcaaac
    37081 caattttagc aacacgtgta atggctcctg ctttactcga atgtgcatac acaatatccg
    37141 gattatattt ctttatcagc tttctaataa ttacgattgc ttttaggtca ttgagatcaa
    37201 tttctctagt caatttaatt tgcttaaatg agagcacaag atttgcataa tccgtctcgc
    37261 aaaaatcttg cgagcaaatc aatatgtttt caaacttttc cttatccaaa tactttaaaa
    37321 gcatgcgaat ataacaatct acgccacctg cagcttgggc aatgtgtaaa actcttattt
    37381 tttgacgcat ttctgtcctc cattttactt atcataacaa acagtatatc tcgaattacc
    37441 agaaaaataa tcttccaatc agcaaagaaa actctcttat actacttcac attgatctcc
    37501 atccattctt taaaggaaag ttcatttcaa ttcagagcaa tctgccaata atagctaaga
    37561 tcaggctgta catacaattg ctgtctttca tagcctccat actgcttcat ctttctcagc
    37621 aatccagatt tgggacccac gatattcata tcttccttga aaatattcca aatctgagga
    37681 gattcatcat ccacgaacac gaaacgaaac ttatagaact tgaacaattt accatttctt
    37741 ccaactctat cttgtgcgaa aacaggagtc cgccggatca tcaaacacaa taactaacgc
    37801 tgtaatcagt atcacaggac acaaatagca agtgttaaaa tggaaaatat gaatacttct
    37861 ccaatatacc cggactagat aactcccttt tgttcataat ccctcatact catcagattc
    37921 accaaacttt accgtttatt tttttcaata gttcaccaaa tgactccatc taacaaggtc
    37981 aaatataaaa aattaacgca ttttcattat cttttaggca tttattaatg gagatattta
    38041 gtgtttttat aacataataa atgctcaatg agagcctatg ttgggtacat caatattaaa
    38101 gtattcagca ctaatatgtt taagatcgat attatatatt tcataatttt gaaaattttc
    38161 tttcatatag gaaacatgat actgaattgc tttagtcacc tttctttttt tagattttct
    38221 acaagtgatt caaaacttcc ataattacag ttattacttt tgaaacgata atatgaacat
    38281 gtgtaatctt ttttatcaac aatctatcaa ttctatccct tactaatatg ccccatttgg
    38341 ttttataacg atccaaattg tcttgataat tagtttaaaa tcattcaata gactttgatt
    38401 agttatatat tctaaatcta aatcaaccat tgcatgaaaa ctaacatcat ttctaccact
    38461 aacttgccac aaacctgaac atccaggttt tacatacaag cgttgaagat catatctcgt
    38521 gtactcagct acttcccttt ccaaaggtgg acgtgggcca actagactca tatctccttt
    38581 taaaacattc catagctgtg gcaattcatc aatgctttta gcgcggataa ttttacctat
    38641 ttgggtgaca cggggatctt ctttcatctt gaacattgcg ccttctattt cattttgatc
    38701 aagtaactct tccaattttt cttcagcatc taaacacatc gaacggaatt tatacatttt
    38761 gaatgttttt ccatttttac caacacgtgt atggctaaaa aatatcggac tccctggctc
    38821 ttcttttctg attcgataag ctgcatagaa catcaaagga gaagctaaaa tcaaaccaat
    38881 gatgcttcct acaatatcaa tcattctttt aaaaaataaa tagatatatc gtttttcaac
    38941 tttttcttta tccaaccaaa cttgattagg atgtacttta ctaatacgag ataataaatc
    39001 ttcactttcc atactttcag tccctttcgc tctctattca tttccttttt atctacgaat
    39061 aaaaactaaa ataaaccaaa cttcttcttt tttacctctt catatattgg ataactgatc
    39121 tcatccccgt tgatcagatc ctttgcgact tgcttcatct gtactacttt ttccttacca
    39181 aagtccttgg caatctgctc atagcactct tttaagtaaa aggtgcgctt agagacccca
    39241 tgggcatcag aagcagccat ctgcacgaga ttatgtttga ccatcgtttt ggctgttttc
    39301 tggatatctt tgccaaatac cccaacataa cttggcgcag tgagttgcgc caaacatccc
    39361 atatctagga acggaagcaa atgattcggg tcttcgcgaa aatgcgcgtt acgttctgga
    39421 tggacgatca ctggtgtctt tcctagttct aacaactcaa aaaaaagctg ctctgcatat
    39481 aacggcactt ccagtgtcgg aaattcgatc agaagatatg tatcatctaa atctgtaaat
    39541 aaaatttcat ctcgctggat gtcttcaatg agcgttccgg taatacgtac ttcttgtcct
    39601 tccaataacg tcaaaggcaa ttgtcttttc tctaattctg cttgtaacga agcgactaga
    39661 gaaatgactt gtgatttttc attgctgtat tttccattat tgtggtgtgg cgtgcataaa
    39721 atatgcgtga tgccttgctg aatcgctttt tcagccatta cgatacttgc ctctaggttt
    39781 tccgccccat catcgattcc tggtaggata tggcaatgta gatcgatcat ttctcttgcc
    39841 tcctctcttc ttatttctta atttccgtag taataaccat cgtctttgct gtgttctgct
    39901 ccgttgtata caacacctag gactcgtgct tgtaccatct ccaacagctc ttttgcttta
    39961 gtcaaggatt ctttacgtga cgtattttca cgtaccacca ataaggtgcc atccgttttt
    40021 gaagacatga tctgtgcatc tgttacggct actattggtg gcatatcaaa aattaccacg
    40081 tcaaataact gtcttgcttc tgctaggatc tgatccattc ttggcgaact taacaattct
    40141 gatggattgg gtggcttagg tccactaggc aaaatcgaca agttctcgac tggcgctcgt
    40201 tggatcacat ctgctacact tcctgatgaa ctaagtgctg tacttaatcc actggcattg
    40261 ttcagctgaa atgtcttata gatgacgggt ttacgtaaat ctgcatcgac tagtagaact
    40321 cgttgaccag atttagcaaa aacgactgca atgtttgatg cagtggtaga ttttccttct
    40381 ccaggacctg atgatgtcac gacaatcgtt ttgatttgct gaccagttgt ggaggcaaat
    40441 tggatattcg tccggatcgt acgatattgt tcggcgattg gtgaagatgg gtttgttaac
    40501 gtgatcaaac tgactgcatg agttggtgtt gctttttgtt tctgtgttcg tgccattttt
    40561 cttccctcct aaactcttga gcgacttctg cgtgatgagg ctgttttatc gtttgtaact
    40621 tttggtcgtg taggagccag tcctttttta cggataccag ctttcaattc tttctctgac
    40681 atttctggaa ctgttcctaa gattgggaac cctaatgtat ccgtcacata tttttcatct
    40741 ttcactgttc gatcgagtaa ttcaaataag aatgccagaa ctacacctac catcattcct
    40801 aacattaagc caatcactag attcaattta ttatttggcg aaataggtgt cgtattggca
    40861 actgcacctg aaacgatcga gattttatca acattcatca cgtcttttgc attctcttgg
    40921 aaaacttgtg aagttgtgtt agcgatctgt tgagcagtca ttgcactcgt gcttattgct
    40981 tgaatcgaga acatttgtga attttctgat tgattgattg agatggcatc cttgatttgt
    41041 tcaggagtca ttttcacgtt gtcttctgtt ttcaaacgtt cttggacttc atttagcact
    41101 agatcactga cgatcatatc tttatacgta ttgatcattt gcaagttcgt attgacatca
    41161 ttgacatttg cagtatctga ttgcggcagt gtcacaatca actgtgcttg cgaactgtat
    41221 tttggcgtca aaatgaaaaa cgttacaatt cctgagatag ccaagccagc aaacatcgag
    41281 atgatgattg ttatcaagtg tttgcgtaat gtttcaaaaa tttcttccaa gctgattgtc
    41341 tcttccattt tttctccact ttcctattta ttctctaaat tcatttgttc ttttaattct
    41401 tttttcacac gatcaagttc attgtcatta actttttgat atgagatgtc atcttgcatg
    41461 aagccttctc cttgcatctg atcttgcttg atcgtatcaa atgcttcccg ataatctaaa
    41521 gcaatctttt tcatttcatc aaagctcatg tctgttttca tattcgactc tagagcattc
    41581 aaaatggatt gatagttgct gacaccattt agactcaata ctttttttgc tacgccttct
    41641 acgatctttc tttggcgccc ttgtctaccg taatcaccat ttgggtcttc ataacgcata
    41701 cgagaataag ataatgcctg aaccccgttc aatgtaattt taccgattgg aaagtcatag
    41761 ccatcttgtg agaaagtgag gtcattattg acttcgatac caccaactgc atcaacgagt
    41821 tcctttagcc ctttcatatt gatagagaca tagtgatcga ttgggatgtc tacatatttt
    41881 tgtacggtat ccatcgccat ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct
    41941 tttgtgtcac gacctacgat gtcgacatac gtatctcgag gaatgctgac gatcgttgtc
    42001 tgtttgtctt caggactgac tgtggcaacc atcatcgtat ctgatcttcc ttgatcgacc
    42061 cgacctaaat ctccagtatc aatccccatc aatagaacag agaaagattc ttgtttgttc
    42121 agatcaactg gtttcgttcg ttctccttgg gttcgttcca ctgactcgta agttttctca
    42181 atagaatgtg agagatccag atataatttt gctcctactc ctacaattgc taatactaat
    42241 acgataagaa ctgctaaaat aatcagaaat atttttttcc ctcttgattt tcttcttcgt
    42301 cttgccatag tttcctccgt ttgctagctt tgctttaatt gttactaact tatattgcta
    42361 tagcccattt gctgagcaac agtttgtgct tttgttgcgt atgctgcgcg tattttttca
    42421 ttccggatct gctctatttg actgagcacc gtcaaatatt gatcgtaagt agcggtggca
    42481 gtcacttgac catccttcaa catcgtatcg aataactgat ctagttgcgt gacttgcgcg
    42541 atctgagtat cagcgaatcc tagatattgc ataacaaccg ttttccaagg actatcttta
    42601 aaaaagctca tattttcacg tacacgagcg acatctgcac ttgtcacctt ttctttgatg
    42661 acgacatcgt ctacagcttg ctgccagttt gatacatttt tagtaaataa atcgttgaca
    42721 gctgtctgga tcttcagttt tgattctgca tcctctaact gcgttaagac tgctttcttt
    42781 tcttcttgga tcgcagcagc ttctttaggt aattctgatt ccttgatgtc ataatcatct
    42841 gccgaaagtt tgatagaaga tagtttatgt gacaaatttg aaactgtttc ttctgttatt
    42901 tcaggattta ggaaaatttt ttcttttgta ctgtacagct tatctacttc ttttgataaa
    42961 gaggtcacgg tgtctttttg attggtgatg gcagattctg cgtaattgat ccgttgatga
    43021 tgttgccgct gattattgaa atggtatcca aacaagcaaa tcacaatagc gcctgtgcct
    43081 accgccacag tagccaataa cttgacgaac ggcttttcca tagattcact cctaaatatt
    43141 gatgatagat aaataaagat gtgattcatt cccatcagaa tgagcgcttt ttattttaaa
    43201 agcataatta aactaatata ttcatagaaa tatttataaa caggttttcc ctatataatc
    43261 ataccacaaa aagttttctt gactagggaa attcctaatt taaaagtaaa taaaacagta
    43321 acaagatcat gattaatcgt tttcattttt atttcctctc ttttcatgag c
//
LOCUS       DASOUD010000010        40458 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.010, whole genome shotgun sequence.
ACCESSION   DASOUD010000010 DASOUD010000000
VERSION     DASOUD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.010"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..187)
                     /locus_tag="U4A29_000552"
     CDS             complement(59..187)
                     /locus_tag="U4A29_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO0679699.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="U4A29_000553"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="U4A29_000553"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO0679700.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="U4A29_000554"
     CDS             complement(1674..2729)
                     /locus_tag="U4A29_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO0679701.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="U4A29_000555"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="U4A29_000555"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO0679702.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="U4A29_000556"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="U4A29_000556"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO0679703.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="U4A29_000557"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="U4A29_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO0679704.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="U4A29_000558"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="U4A29_000558"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO0679705.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="U4A29_000559"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="U4A29_000559"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO0679706.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="U4A29_000560"
     CDS             10491..12050
                     /locus_tag="U4A29_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO0679707.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="U4A29_000561"
     CDS             complement(12161..14092)
                     /locus_tag="U4A29_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO0679708.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="U4A29_000562"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="U4A29_000562"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO0679709.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="U4A29_000563"
     CDS             complement(15021..15773)
                     /locus_tag="U4A29_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO0679710.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="U4A29_000564"
     CDS             complement(15932..16141)
                     /locus_tag="U4A29_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679711.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="U4A29_000565"
     CDS             complement(16152..17480)
                     /locus_tag="U4A29_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679712.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="U4A29_000566"
     CDS             17616..17771
                     /locus_tag="U4A29_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679713.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="U4A29_000567"
     CDS             complement(17845..18432)
                     /locus_tag="U4A29_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO0679714.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="U4A29_000568"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="U4A29_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO0679715.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="U4A29_000569"
     CDS             complement(20012..21301)
                     /locus_tag="U4A29_000569"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO0679716.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="U4A29_000570"
     CDS             complement(21455..22387)
                     /locus_tag="U4A29_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679717.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="U4A29_000571"
     CDS             complement(22544..23203)
                     /locus_tag="U4A29_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEO0679718.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="U4A29_000572"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="U4A29_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEO0679719.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="U4A29_000573"
     CDS             complement(24244..24534)
                     /locus_tag="U4A29_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679720.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="U4A29_000574"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="U4A29_000574"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO0679721.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="U4A29_000575"
     CDS             complement(26821..28176)
                     /locus_tag="U4A29_000575"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO0679722.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="U4A29_000576"
     CDS             complement(28200..29369)
                     /locus_tag="U4A29_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679723.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="U4A29_000577"
     CDS             complement(29366..30253)
                     /locus_tag="U4A29_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO0679724.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="U4A29_000578"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="U4A29_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEO0679725.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMTIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="U4A29_000579"
     CDS             34320..34841
                     /locus_tag="U4A29_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEO0679726.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="U4A29_000580"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="U4A29_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="U4A29_000581"
     CDS             complement(36284..36940)
                     /locus_tag="U4A29_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679727.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="U4A29_000582"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="U4A29_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO0679728.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="U4A29_000583"
     CDS             complement(38477..39475)
                     /locus_tag="U4A29_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO0679729.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="U4A29_000584"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="U4A29_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO0679730.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
BASE COUNT        11051 a         8799 c         7123 g        13485 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagtcat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatat
//
LOCUS       DASOUD010000011        39014 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.011, whole genome shotgun sequence.
ACCESSION   DASOUD010000011 DASOUD010000000
VERSION     DASOUD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.011"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(714..1340)
                     /locus_tag="U4A29_000585"
     CDS             complement(714..1340)
                     /locus_tag="U4A29_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679731.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1635..1970)
                     /locus_tag="U4A29_000586"
     CDS             complement(1635..1970)
                     /locus_tag="U4A29_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEO0679732.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1957..2241)
                     /locus_tag="U4A29_000587"
     CDS             complement(1957..2241)
                     /locus_tag="U4A29_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEO0679733.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(2297..3820)
                     /locus_tag="U4A29_000588"
     CDS             complement(2297..3820)
                     /locus_tag="U4A29_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO0679734.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3813..4988)
                     /locus_tag="U4A29_000589"
     CDS             complement(3813..4988)
                     /locus_tag="U4A29_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO0679735.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4992..5177)
                     /locus_tag="U4A29_000590"
     CDS             complement(4992..5177)
                     /locus_tag="U4A29_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679736.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(5143..6837)
                     /locus_tag="U4A29_000591"
     CDS             complement(5143..6837)
                     /locus_tag="U4A29_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO0679737.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6834..7307)
                     /locus_tag="U4A29_000592"
     CDS             complement(6834..7307)
                     /locus_tag="U4A29_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEO0679738.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7376..7531)
                     /locus_tag="U4A29_000593"
     CDS             complement(7376..7531)
                     /locus_tag="U4A29_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEO0679739.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7665..8045)
                     /locus_tag="U4A29_000594"
     CDS             complement(7665..8045)
                     /locus_tag="U4A29_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO0679740.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(8049..8264)
                     /locus_tag="U4A29_000595"
     CDS             complement(8049..8264)
                     /locus_tag="U4A29_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679741.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(8267..8674)
                     /locus_tag="U4A29_000596"
     CDS             complement(8267..8674)
                     /locus_tag="U4A29_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEO0679742.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8940..10415)
                     /locus_tag="U4A29_000597"
     CDS             complement(8940..10415)
                     /locus_tag="U4A29_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEO0679743.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10405..11253)
                     /locus_tag="U4A29_000598"
     CDS             complement(10405..11253)
                     /locus_tag="U4A29_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEO0679744.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(11290..11652)
                     /locus_tag="U4A29_000599"
     CDS             complement(11290..11652)
                     /locus_tag="U4A29_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679745.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11653..11811)
                     /locus_tag="U4A29_000600"
     CDS             complement(11653..11811)
                     /locus_tag="U4A29_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679746.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11952..12576)
                     /locus_tag="U4A29_000601"
                     /pseudo
     CDS             complement(11952..12576)
                     /locus_tag="U4A29_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12573..12884)
                     /locus_tag="U4A29_000602"
     CDS             complement(12573..12884)
                     /locus_tag="U4A29_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679747.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12928..13221)
                     /locus_tag="U4A29_000603"
     CDS             complement(12928..13221)
                     /locus_tag="U4A29_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679748.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13415..14062
                     /locus_tag="U4A29_000604"
     CDS             13415..14062
                     /locus_tag="U4A29_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0679749.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            14123..15268
                     /locus_tag="U4A29_000605"
     CDS             14123..15268
                     /locus_tag="U4A29_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO0679750.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15349..15654)
                     /locus_tag="U4A29_000606"
     CDS             complement(15349..15654)
                     /locus_tag="U4A29_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO0679751.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15679..16110)
                     /gene="ruvX"
                     /locus_tag="U4A29_000607"
     CDS             complement(15679..16110)
                     /gene="ruvX"
                     /locus_tag="U4A29_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO0679752.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(16107..16373)
                     /locus_tag="U4A29_000608"
     CDS             complement(16107..16373)
                     /locus_tag="U4A29_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO0679753.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16628..17353)
                     /locus_tag="U4A29_000609"
     CDS             complement(16628..17353)
                     /locus_tag="U4A29_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO0679754.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17714..17784)
                     /locus_tag="U4A29_000610"
     tRNA            complement(17714..17784)
                     /locus_tag="U4A29_000610"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17750..17752),aa:Gly,seq:ccc)
     gene            17901..18653
                     /locus_tag="U4A29_000611"
     CDS             17901..18653
                     /locus_tag="U4A29_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO0679755.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18669..19148
                     /locus_tag="U4A29_000612"
     CDS             18669..19148
                     /locus_tag="U4A29_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO0679756.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19399..20979)
                     /locus_tag="U4A29_000613"
     CDS             complement(19399..20979)
                     /locus_tag="U4A29_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO0679757.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20972..21715)
                     /locus_tag="U4A29_000614"
     CDS             complement(20972..21715)
                     /locus_tag="U4A29_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0679758.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21891..22667)
                     /locus_tag="U4A29_000615"
     CDS             complement(21891..22667)
                     /locus_tag="U4A29_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0679759.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22915..23751)
                     /locus_tag="U4A29_000616"
     CDS             complement(22915..23751)
                     /locus_tag="U4A29_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO0679760.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23762..24691)
                     /locus_tag="U4A29_000617"
     CDS             complement(23762..24691)
                     /locus_tag="U4A29_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO0679761.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24883..25236
                     /locus_tag="U4A29_000618"
     CDS             24883..25236
                     /locus_tag="U4A29_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO0679762.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(25310..25966)
                     /locus_tag="U4A29_000619"
     CDS             complement(25310..25966)
                     /locus_tag="U4A29_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO0679763.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            26192..28126
                     /locus_tag="U4A29_000620"
     CDS             26192..28126
                     /locus_tag="U4A29_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO0679764.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(28212..29435)
                     /locus_tag="U4A29_000621"
     CDS             complement(28212..29435)
                     /locus_tag="U4A29_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO0679765.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(29429..31327)
                     /locus_tag="U4A29_000622"
     CDS             complement(29429..31327)
                     /locus_tag="U4A29_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEO0679766.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(31321..32439)
                     /locus_tag="U4A29_000623"
     CDS             complement(31321..32439)
                     /locus_tag="U4A29_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO0679767.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32522..33355)
                     /locus_tag="U4A29_000624"
     CDS             complement(32522..33355)
                     /locus_tag="U4A29_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO0679768.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(33348..34265)
                     /locus_tag="U4A29_000625"
     CDS             complement(33348..34265)
                     /locus_tag="U4A29_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0679769.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(34492..35616)
                     /locus_tag="U4A29_000626"
     CDS             complement(34492..35616)
                     /locus_tag="U4A29_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO0679770.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35799..36707
                     /locus_tag="U4A29_000627"
     CDS             35799..36707
                     /locus_tag="U4A29_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0679771.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            36848..38461
                     /locus_tag="U4A29_000628"
     CDS             36848..38461
                     /locus_tag="U4A29_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO0679772.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        11814 a         7770 c         6414 g        13016 t
ORIGIN      
        1 ttttagcgat tgttactctt ttcttttatt atacagtaaa agaaacaaac gaggcttcga
       61 aagcttttct actttttaag gaaatttagt gctaaacgaa aagaggttgt gaaaaagctg
      121 tcctgcatca agtaataaga acagccaaac aaaaatagct cctcataatt ttcgtttggt
      181 tgaacttatt accgcagatg caagcttttg aacaccgttt aagaaagagg tcgtgagcct
      241 gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa
      301 tattgtgact tgtgaccgag gagttggcag gagaacaccg tttaaggaag agactgtgaa
      361 gtcaacgttt tgacctgatt attagagaaa caaatggcaa aatagttctt catatttcgc
      421 cgtttttcga gcgaatgccg aggggcagct gattgaacat cgtttatttg gaaaaaggga
      481 ttgtgacaag acgtttgtca caatcccttt ttttatatct tattaattca ttcttttgtt
      541 ctattcttct tcttccgcca tgaatgagaa acccatatca aagaactata aaggttatca
      601 aaacagcgtt tatgactaaa tgaattagag ttatctatca ctatttttca agcgatttaa
      661 accaaatggc atacaatatg gcatacaaaa acttcaataa gccgtggggg tttttaaatt
      721 tcttcttgtt ctttaaaatc aatgagtgga gctacataat ttgaatccac tttttcaaac
      781 gtcacttttt gcatcgacga atgttttctt tcccaagata acgtaaatag atttgactct
      841 ggacttataa agtctacttc accttctagt attactttct tatgcaaata ttcctctaat
      901 aaatatagac ttgctaaagc atacaacaca ttttccaaat ttgcttcctt ataatttgaa
      961 gaacgaccat gttttactct gttatatatt ttccaccaat ctggtgtatc ttcaggccac
     1021 ccatctccaa aaggttttag tgaagtcaat gttaaagaat tcataaccct aacaccatta
     1081 tccaaacaca ttaattcgct agatttaata acagctttaa aatcagttat gcttttattg
     1141 ctactgctat caaatccgca aagttctttg aaaataatct cacactccga accaatcgct
     1201 agtagtagtt tttgatattc aatagagaat gttttgaaat tattctcatc gagcatcaca
     1261 aaatctgtgg tattaataaa ctcattttct aaatgtagat aatacttcca ataaaaattt
     1321 gtaaactctg ctttgtccat taaatcacct cttttgaatt ataacaaaaa aaacacgtcc
     1381 acaacagacg tgttctttga tttctacaca atcagcttgc caaaaccatc caatcagtcc
     1441 tagcatgcct gtattaaaat tgaacgttca tcatctatca tctttcttag tccagcatgt
     1501 ttgcaaccac acctacaatt ttcagtccaa caatagattt cgactagcta agtcttgcaa
     1561 aattcaggca gctaaccctt catttactac cttaattata ctaaataccc acaacgttta
     1621 caagtgtaat cattctaaga aatctgttta gctatgatcg ttgtataatc tttaaacgcc
     1681 gttccttcat tgattgaaat aattttataa ctcttgtttt tccaagctac tttcatatcg
     1741 tttgttagtt cataatcttg atcgtatcga ataataaacg tcaacgttcc ttcaagaatg
     1801 gtacctacac tagcaagcac gtcttttagc atttgtgaac gcacattagc ccaacattca
     1861 aatacagttt cttcactatc caccatctgc ccatcttcat cttttacccg ttttgtcttt
     1921 ttaaacttga ctctctcagt caattcattt acattcttaa tgattggcat aataagcacc
     1981 tctcatttgt tgtactaatg cttgtaccgt tactggtatc cgttcgctgc tggcttcttg
     2041 acggttcaaa taccaatgtt gagtaagtaa tgacaccgcc aaatcaaatt gctgataatt
     2101 atccatatct ttatccgttg cgctactatc aatagcactt tttatatatt cgcctgctgt
     2161 atcaatcaat tgttgaataa agtcatcatc aatagtgtga tcgatccgca tacttttttt
     2221 tatgctatcc aaaattacca ttttatctct cctaatgaaa aaaaggcagt gggataaacc
     2281 gccgcccaat atttttctat acttttggtg ttacaactgg cgtaaactca atatacactg
     2341 aagcattttg atcaattttc ttataatcgt tgcgaacgat cactgcaagt ccttgtgagt
     2401 agtaatcgaa tttttcccat tgagtcgtta cttgatttct acgagcaaca aagatagatt
     2461 gtgcaatatc ccccataatc atagggaatg ttccagcttt aggattggca aacaatgtat
     2521 ctgcaatcaa tactactggc ataccgaata atgatttacc actaggtgct gtcacatcgg
     2581 gttgtaaaat gtaacgtcca tctgaatctt tcaatgtatc taggtggtaa tatccgcttt
     2641 ggtttagaat taccatttta tttaatgctg ggtctaatgc cacattgtat agttgtttca
     2701 aatcgtccaa cgtagcagcc gttttcttag tgaatgtttt taataaatcc atgatatgct
     2761 tattgtcagt gttttctacc aatttcgcta actgatcttt gacctcttgt acaatattca
     2821 ctgctgaatc ttccacaacc tcatttgata aggcaatctt accagcacga gtttctactt
     2881 tataatcaac tgaagtaaac atttcagcgt caatatcacc aatttcagca agttcagctt
     2941 ttgttgctaa cactgcttgt tggttagttg ctactggata tttaccttgt ccatttgata
     3001 ctgtttttac tgttgcatat tgagctaagt tataatttga acgtttcaaa tcaaagactt
     3061 caccgattac ttcttcggga acaactgccg ctgcatttac agtagtgact ccatcacgca
     3121 cttcgccttg tgaacggata tattcttcat acccacgcaa ttctgttttt tcgttatcga
     3181 ttaaagtttt ttccatgtta ttactctcct tttgtgcttt agaagtggat tctaagctat
     3241 tgtttgtttg ttttgcttgg ttgttactca taaacgattc gtaagaacgc ttatttactt
     3301 ggacatttga tgaatcataa gcgggaacgg tcacgacact aatttcatta agtgctttga
     3361 ttttatctat tgatcgttca attgtgccat cttctttttt gtcgaatgaa tctaatccaa
     3421 gcacgaaccc aaaggacata gaatcaacaa ccccttttga gatattctca tacacatcat
     3481 ttgaataggt cgtgtcggtt agctctgctt caaaatgtaa tcctgtatca tcgacattca
     3541 atttcaacgt acctgctttg acactagcta gtggctttga ataatcgtgt ccatataata
     3601 agaacacatt tgataaatcc acctctttta atgcttcggg tgtaatcact tctacaaagc
     3661 cgcctaaatc ttttgacggt tgcccgaatt tcaacgcata gcctgaaata gttttgccgt
     3721 ctgttggttg tttttcattt tctttctcta ctttgggaga atcggctgtt agctcagctt
     3781 cttctgtcag ccgtttttcc ttttcttctt cattattcaa ctggtgtaac tccttcctgt
     3841 tcttttaatt gatatttctc caacgtatct aaatacgtaa agttcaaact tgctagtaaa
     3901 cgatcgccac catccatggg gggtagcccc attttcgacc gcccctcatt aatcgtgaga
     3961 agtgaccctt tgacctgatc caatacattt tttacttttg tttcaggatc agtctctagt
     4021 aactgatcgg aattaaagcg gatattggtt tcaagtttcg tatctaattc actcacaaac
     4081 acattaaagt aatgaatcag cgtactttgg atatactgga gattactttg aacggtgcta
     4141 gaatgttcat tttcaacgcc taaacgctca atcggtacac caaacgcctt ggcaatttgt
     4201 tttgtgttcc aatcattgga attgataagc ttcaaaacat cggtgtttat ttctaatgtt
     4261 ttgtagtcca ttgtttcatc aagaatgatc gttctaagag cattatctcc actacttgaa
     4321 ccgttggctt cttcaaactt ttcacgaatt gcgttctttg ctgatccgtc taaatctgac
     4381 ttatgaacct tcaaaatacc tgacccactg acaccacgag taaagaaatt gtgcaatgtg
     4441 cggttgccag cttgttgaat tttcagttca tcacgtaacg cataaagtgg tggtaatcct
     4501 gttaaaccat cttgggtgaa atatttgaag tgcaaaatat cgctagactt cacacgtctt
     4561 tttttatcac caatctgata agacaacgta ccattatcta attgagtaac cgttacactt
     4621 gagttaggta ataagtgaat ttcttctacc ttttcaccgt tgcgtttaat ctctgcaaag
     4681 ctattaccgt ttagcaacat attgaccgcc aaagcaaact ttaaatgcca accatccatt
     4741 tctgaattag gtttctcgtt tagtaacttc accagttcat tatcagcttg tggcatattg
     4801 tttttgacca attgaatcgg acttgaagca atatcactag caataattcg aacggctgca
     4861 aacacatcac tatttcttaa tgcacgaacg cttgtaaagc tggtggtgta atcatccgat
     4921 tggattgata ccacatgttg taggaaagga tctcccgttg tattttgtct cgctttaaaa
     4981 aatgccatct tttaacctcc cttctgttct gtatttagca tgaccgctac cccaataagc
     5041 actatgccca gcgctaacaa gccatagtag acatttgtta gtaaggtgat agcaaccaac
     5101 acacacacca atccaagaag taaaagaacg gtatgaatgt aattagaaag aaaattcttc
     5161 acttgtataa aattcattgt ctgctttcgc tccttccgtt gtggtgaagt aatccattgc
     5221 aaacacatag gcattgatta atgcagctat tggatcaatt ttgttactgt tctttgattt
     5281 attaatctga atgccgttgt tatccgtttt taaaatagcg ttgttcactg catgggtgag
     5341 aatcgtattt ttattgtgaa taacgttgcc ttcataaact tgttctctaa aagttcgggt
     5401 cggaacgttt agagtaatcg ttccttgtct tacttctaac attgggtagt tggctttttc
     5461 agctttactg ataagcgaat tagcgttata cgggtcataa caaatcccca tacattccaa
     5521 atcattttct tgaatcaaat cttgaataaa ctgaaatact tggtcatagt ccacgattcc
     5581 actttctaat tgagtaatgg aacactcacc agctttttct agttctcgat aaggcaaacc
     5641 gtcgcgcttt tctttgtctt gaagcccata tttggtggct acaaagctat gtgaatcaca
     5701 ataaagttcg ccgttatcta gcggtacgat ccatgaaacg ctagttaaat catctgtttt
     5761 agataaatcc actccgatat aaacgggctt gcctgtaata tcttgtggtt ctacttcggt
     5821 tgcctgccag tcttcagcaa tcatatagct gtcctcactc gcttgtctcc ataaattgaa
     5881 gtttttcacc aatacagaat taaggttatt ttgtttcaaa gcaaggttca catcatcttg
     5941 aatggttgga atcattactt ttttgatttc ttcactttca aaaatcggat tggctttgat
     6001 ccatttctct tgatcgtgaa tttcttcggg atcgtccaat tcccatatcg ctataaagta
     6061 cctgtcggct tgttcttcgc cttttagcac acgatctaac aataagtatt cctcgtacat
     6121 aggaacgttt aaatcaagcc cagcggtact aataacacac aataagccgt tcttttgttg
     6181 ggtcattcct gacttaatga cgttgtacgt tttacgagtt tttgcttcgt gccactcgtc
     6241 aagaattgcg gttgttggtg caaacccatc tagcgtgctg gtttcactag ccagtgccat
     6301 agcgaacgaa ttagagggca agtgctgaat cttagagttc ataattttca gttgtggtct
     6361 taaaaactta ctagacttga ccacgtttct aagtgaatta gacagcatat catagcccaa
     6421 tttagcttgt ttcaaagcgt tacttacaaa caatacttgc cttgcttctg ctggttcttg
     6481 ttccataatc aacgcattgg cagccatgcc acttgcgata tacgttttcc cattcttacg
     6541 gctcatactg gtaaacgctc gattgaatcg cctgtaattc ccttctttag tacgccagcc
     6601 ataaagactg cctagaatga atttttgaaa tcctaacatc ttgattgctt taccgtccgt
     6661 agatggtagc aactcaatga atttaatcgc tttggtggct tgttcttcat cgaacacata
     6721 agggaagcta tctgacttag aacgctccaa atcgcttata tgacgctcac acgcttggat
     6781 aattttcttg ggtgcaatca gcttacctga taggactttc tcaatgtaat cattcatgaa
     6841 ttcatcaact ctttgaacgg gtcttcgggt tcatcatttt taaccttgtt caaagctagt
     6901 tttgatcgtg attctaaggt catacctagt gaggtggctt gttttttcaa atcactcata
     6961 gcttgtgatt gaacccgaac cgctggattg gctttcttta ctccgttttc gcatgtgata
     7021 aacgtacctg attttcttat ctcggcttcg gctgttttca ttcgagcaaa ggctaaacaa
     7081 taggctacca atgaaccata atctgcttca ctcaatggat aatctttttt taatgttggt
     7141 acgatacgat tccattcact tctagcgctc gctgccatcc agtcaggggg cgtgatattt
     7201 attaatggtt gctgcttgaa caattcttct ctagcgtcca cacggattgc tttttcttca
     7261 cttgatatat tgccttttgt atcgtgtaat attttagggt gtcccatgtt gtcctccttt
     7321 cagacaaaag aaaaaggaac gtaagcaatt aagctcacat tccttcgttt ttcgatcaga
     7381 atttttcttt atctaattta tctatataaa tgatttttcc gttctgcatt tgaatggtca
     7441 cttgaccaaa atgaggtaac tcaatggtgc gctgtttccc tgttttatca gtaataacaa
     7501 ttgtattttt caataaatct cctactttca ttatatactt tttcacttgt gattacaagt
     7561 gtaatcgtta tttgtaatta cccttatgcc ttttcacaac ggttatttgt accaaaatga
     7621 gtaggcatcg attgtcagta agtttcacac tatgccccct taactcaaag ggtactcttt
     7681 tctcgtcttt gtctttcatt ggctgtcttg gaattatggt gtgcatggca taaagaacga
     7741 aaattagttt catctaaccg tctattccaa tcatctttta gttcaatttt gtgatctaca
     7801 acatcagctt tccttatcaa gccttcttct aggcaatcct cacatactgg atgatttaac
     7861 cgatacaagt atgaagcctt tcgccatgtt ctactttgat agaattgaaa atatttacct
     7921 tcgctttgct ttctttcttc acgcttaatt gccgtttgct ttgctttatg cttcccacaa
     7981 tacttctgcc tataatcaat caatgtttta catccagcat gattacattg ttttttaact
     8041 gccatagatt accaaccaaa gcgatctcgt tcgtcagaat catcttgttc ccattttgat
     8101 ttgtattcaa tataaggcga aactatcttc aagcacgttc cgtctttaaa attaacttta
     8161 gtgattttat cttcctcaaa actaataaag tctacatctt cttgagtgtt tgaatactca
     8221 atgtgtttct caccatcgaa gtatctaata ctttccatat gcataattat tctacctcca
     8281 ataaattagt tatcaattca tcatctgtta gtagcaaagc caagaactta tcatggatgt
     8341 ttaaaaacaa attatcctca ttcgatgtaa gctctcgttg tctcgctcga tcatataggt
     8401 taagcagaca attaaattca tccattagct cacctgtatc atcatagttg ccactatcta
     8461 ccaatgatcc cactactagc gattgacacc acgctactga ctcctgcagc caatcaggtt
     8521 cttcttccat tatctttaat acttctggat caaatgtgga ataatcttct gttaccaatc
     8581 ccacactaaa tgccagcgtg ttcattagaa accgatccat ttcatctgta tattgaaagt
     8641 tcggtgcttt gtttgttgaa ataatattga gcatttactt tcctccttga ttcgtaacac
     8701 gtggtaacac ctacgtaaca ggtacccgtt accactcaat cccaacgggg acaagggttc
     8761 tagcgttttg gtaacaggta acgggtaaaa tccagtaaca ttattcccca gcatattagc
     8821 taactatttc aaaagtttta aggaaataag gtgttacccc gttaccaaat tgctgtatcc
     8881 cttggggcag taagggtaaa ctagtaacgg gctaggtgtt actgacccgt tactacccgt
     8941 tactttttgt actttccagc tcgattttta ttttcatagg catttgactt attgtaaaat
     9001 cctcgtcgtt tatttctttt tggcagttgc ttccgttccc aaccaggaat actgctaata
     9061 atcaaggcaa tctttttagt ttctgcatct gatcgaccat tcaataaatc ctttgcttgt
     9121 ttatcaaaaa cagcttccaa aatatcagcg gtgagtacgc tatcaactaa ataaaactct
     9181 tgttgcatag tagctccatt atagaaatgc tgtaaatttt tctcattaca tttactgtca
     9241 ggatagttct gataataaga acgtctcgcc cacgctggag cttcttccca tttatacggg
     9301 atttccattt ccaaatattt agtgattgct tctttaattg gattctccat cttggcatcc
     9361 tcttgatagt cttttgcaat ttctaagact tctttatctt catcgggact aaaatagatt
     9421 cgttgtcctt tatcaaaaag cacctttgct tcagctaaca cttgaagaaa atattcatcc
     9481 tttgttttaa acggattcat ctttggctta ttcttacaag gcaaaggata aaaacggcgg
     9541 tttcctgtat catctttcaa atactgccca tcattcgacg ttccaataaa tacacattgc
     9601 cttgcccaag ctatgacatt ccgaccataa ggaggtctaa ttttatcgat cttggcactg
     9661 atgaagtttt ttatctgttc aattttagtt ttgttcattg aagataattc tccaagttca
     9721 ataattacat tgccttgaag ctgcatataa tcatctttct tttcacctag cgaatctaat
     9781 gaatcggtga aaaaatcatc cgtgtaaagt aatgctggaa cggtactctt accaatccct
     9841 tgcggtccat ccaaaactgg aacgatctca aactttattc ctggtttata gatacgtgcg
     9901 actgatccga ctatccaacg ttttgttact tctcgtgtgt aaaggttgtc gtctacccct
     9961 aaaaggtcaa taaacagtgt ttccagtcta ggctgttgat cccatttaac tttttcgata
    10021 cgttctttta ctgggttgaa aacattcttt ctagccacat ttactactgc tttgtgtatt
    10081 ttgttgtcat caaaaactac attgtagttt tcttcaatat atgccgttaa ggcatcctca
    10141 aaactgtctt cccattctcc agcggtacga ttgatccaag gagaatcttt taataggtga
    10201 atgtatccgc taaattcgtt ataagcaatt gttccagcta aattgctatc attggttaaa
    10261 atcaattctg cattcacaac actgttactt ttaatctgat cgtctttatt gcatcttaat
    10321 cgtgacatcc aggttttcaa ttcaatgata ttgtcttcac gctcgctggc ttcgggaaag
    10381 tctaaaatac tatctaattt atgcttatga gccatgagct ttccccctca aatctttttt
    10441 aatcatcgat cgaataattt gattcacttc tttatctttt agcggtggtt cattgtacgc
    10501 atttgctgta taaatcaatt ctgctaaagt ttgattatct acaccgtgcc atagtaacca
    10561 accgcataac ttagttaaat gatcgtttct actaccttcg ccagcgcctt gaacaatcat
    10621 atctaatttt tttgctgtcc ctgtttttgc tacgggtcgg tattgagtct ttatttcttg
    10681 cggttttttt agcagtaggt tcactagcca atcgggagct ggtgcaatat tatttagaga
    10741 tacgttttca ataggttcat atgctctccc aacacttctt gaaataatca tagttggcgc
    10801 tattggaact cctttagtga taatatcaat cccgcttaaa cttttacctt tatcatcacg
    10861 cccgaagata tcagcaaatg cacctgtttc ttgttggata attacaccat caggaatttt
    10921 aaagaagaaa tggattcctt tacctggtgt gatttccgta taagtagaag gaaatggatc
    10981 atacattgaa gcaagttttt tataattctc aacgccgttt cgtgtttcag aatgttgatc
    11041 taggtcaact accaccattt gatgatcttc aagcattaac ccaatatttc tagttgcatc
    11101 ttccgtccac cattcattaa tttgattaga atcgttagtt gcatcattcc aaccactaga
    11161 gccttttatt ggaattttag ttccaggaga taagggataa attgaccatc ccgacttttg
    11221 taattccgtt gctgcttgat aattatttgc caataaacct ctcctccttt ttcaaaatac
    11281 tggtataaat cattcagaca cctccatgag cttcaactgc tcaagttcgt atttcaaccc
    11341 atcccgaaca aattcagcac cttttaattg attctgatac tttacccatg tattaaatgt
    11401 tttcaccctt gcttcttgca tatcagtaat gtttaaccat gcggttaaaa tctgattttt
    11461 tagctcgttg atttctcgac aaattatttt gtgttctgca tgttttttga gtacatacaa
    11521 aactgttagt ttttcattga atgaatttcc aaagtaggtt ttcccgatat tattttctag
    11581 ttcagcgatc ccttgattaa tttcctctaa ttcaaggcta tcgagttggt ttaatagttt
    11641 tgttttgtac atctacttat gccctccagt tcttaattga tagtcgtact cgtcataagc
    11701 agtgaagaga tataacgcta atcctgcaaa gatagctatt tttgcaatta atggtaataa
    11761 gccgaaaatt ccgccgatta aaatgcttcc tataattaag tttcgtttca taatggttcc
    11821 ctccgatttt ttaatatggt ataatcggaa caaagaatat cgggttttat cccaattaag
    11881 ttgcctttat cctgtccgcc aagactgtta tggataaggc tttttctttg ttctcgatca
    11941 tgttaatgct cctaagttgt ctgcaattca gcagaacgat tataaatttt ctcactcact
    12001 gaaaactgca acccatattt ttctttaaca gtcattaatt ctactgtctc atctaaaata
    12061 ggttcacgat caaccaacat ttcaggagtc atatctttct tttttaccat tttggaatga
    12121 ccataaatag ttgatacagc tttattggct atcacattcg ctttaatgta gtctactggt
    12181 tttggttcag taatagcgtg gcttaattta gtcattgctt ctttttggtg ttctttgtct
    12241 agcatgcgga atacttgaaa gccttcaagt cctgtggact ggcgtagttc tttgatgatg
    12301 tcgaaaatcc agtcttgaaa tgcctcggct tcttttttac ggcttttgaa tgccatgcgg
    12361 taaatatttt ttttcgttga tgatgtttac atcttgtgtt ctgcccaatg aatcgatgac
    12421 cttactagta gtaaccccat ctttaggcaa agagtgaatg gctctagtaa cctgtttaag
    12481 acctaacgct tccgcaacat ctttcgccac tgcccaccat tcatcattaa tatcgacaaa
    12541 tcttataatg tgtccgttcc aaatttcagt tttcattcaa cgtcctccag ttcaagccct
    12601 agcaagctcg ctaacaactc aacttgctga tacaaaattt cttgatactc ttcaatagaa
    12661 agtcgttctt taaactctgc ttgtactaaa tcgttcaagc cacgcaatac tccgcttacc
    12721 gttggttctt gtggtagcaa tttttgatag tccatttgtt cgtacaaaca ttcaaacgtt
    12781 tcttctagcg ctttgccttc caagtttgct aattctgccg ctgtgtcttc tgtaagaatt
    12841 gttgataata ctttttcgat tgctttattt tgtgtcgatt ccattattat ttcctcctat
    12901 taatcccttt gcccccacgg cttgcgttta tttttggtat tcttccataa agcgatcaca
    12961 ttcatcttgt gaaattctta cgactccatc aactataatc aactttaaac cgttaggaac
    13021 atatttctgc atcatagtat tgtatgaaac gttcatatac ttagcagctt gtgccttgtt
    13081 catatatttt ggaaactggt actttttttc taataatgaa agtaacgttc cttgagtttg
    13141 ctctacttgt tgaacaactc gatcgtcaat aattttaaaa gctggatcat caagcgtgag
    13201 tttgtcaatt tttaattcca tatttatccc ccctcgttag catacatttc tatacaaaga
    13261 ttttaaaaaa atgcaagagg ctcttgaata catttctata cctcttataa actcaatata
    13321 ccatcatacc ttttcatttg caagaatttt aacatacttt tttgaacttt tatgtttcac
    13381 acatatttaa atcgtgatat agtagagaaa atatatgaat ggtggtgaaa atgtggctag
    13441 aaaaaaatac cccgaagcag tggagacagg acaaagaatt agactggcta gaattgaaaa
    13501 gggactctca atggaacaac ttggaggtaa attatcccca ccggccagta aaggtgccgt
    13561 tagtaattgg gaaaatggct ataatcttcc caataacacc aggcttaaac aactttcaaa
    13621 ggtattagat gtatccacta cctatctatt aaacggatct tatacattaa gagatatgca
    13681 tatgatgccc gaaattaagc gaaatgtgct tgaaaagcaa ttgaaaaaaa ctattgaaaa
    13741 aaataactct ataattgatt ctaatctttc taatgctatc aataacatcg aagaagataa
    13801 cctgactgtc tcacagaaga tttatttaac aaacgcaata aaatatctta aaacaggatc
    13861 tcgaattgaa tttcctgacg ggagtacccc aattgcttat ctcgcagctc ttctaaatac
    13921 gcttttacga tatgactcat taattaatga ttcagaaaaa aatacagatg agaaaaaaga
    13981 gtatcaagaa ttttatgaaa atgatcttct aaaagagttc acagctcttg ttactgagct
    14041 aaataaacac tattcaaaat aataataaac tgcctcctcc aaccgccccc acggcttgct
    14101 acactatgga gggaaaacta ttatggcaac attcaagcaa tatgaaacta aaaaaggtaa
    14161 attttggctc tatgaagcct atcttggaat gaacaaaatg actggcaaac ctgataaggt
    14221 tcgtagacga ggttacaaaa caaaaaaaga agcccagctt gccttgtcac gcttgcaagt
    14281 ggactacgat aaaaacggta ttaaaaaagc gaacaacgaa acgtttaaag aagtttatga
    14341 cttgtggcta gaaacatata aaaccactgt ccgagaagcc accttcatga aaactgaaat
    14401 caagtttaga aagtggattc ttccaaaata tggaaatctt cgtgtgaatg aagtcacggt
    14461 aaaaaaagcc caacaaattg taaatagctg ggctgaaact actgaccaat acaaggtact
    14521 tcattcaact tctaagcgta tttttagcta cgctatcaat cttgggatta ttgagactaa
    14581 tccactagac aacgtattaa tgcctaaaag aaccaccact aagcataaag aaaaggtcaa
    14641 agtttacagt aaagaacaac tgatcacctt gtttgattac ctaaacagca aacctgaaac
    14701 ttatcgaaac gactttgata aaacgttact acgctttctc ttttatagcg gggttaggat
    14761 cagtgaagcc cttgcattga attggtcaga cattgatttt caagaaaata cggtcaccat
    14821 caataaaaca ctaagccaat ccaaaaatgg ctataaaatc agtgatccta aaactgattg
    14881 tagtgctggg gttcttccac tagatgaaag aacaatttct atattaaaaa aatggcagat
    14941 taatcagcgg aaatatatgc tgacacttgg tgtaactgat ccgactatga ttttttgtgg
    15001 tatctataaa gaactaatca cacaccacgc tatttatgca agattgatta ctatttgtga
    15061 aaaagctatg gttccatttc tcggtgtgca tgtagcaaga cacactcatg cttcaatgct
    15121 cttggacgct ggagctacta tggtagaagt tcaaactaga ttacggcata caaaaattag
    15181 tcagacgatc gattgttacg gtcacttggc aaaagaaacg aaagaaaaga ctgttgaaaa
    15241 attagtacaa cacttaaata ttgactagat ggcatacaat atggcataca gtaaaaaata
    15301 aaaaaaggag aaaccctatc atatcaaggt ttctcctagc tttatagtct attcttcttc
    15361 ttccgccatg aatgtattga atacttcttc aatcatgtcc cattctgcgt ctgtttcgat
    15421 ttgttctaac tcgccttctg ttccatcttc gttttcgatg tatgcataag cttgtaattc
    15481 tacatcttca tcttctgaaa caccagcagg atataaaaga acatagttac gtccaaattc
    15541 ttcttgtccg tcaactgtta gtaggatttc atataatgtt tcatttcctt ggtcatccac
    15601 caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg ttatgttctg tcataagatt
    15661 acctcttttc ttttattatt aaaataattg cttacctgag ccgtctaaat aattttgtaa
    15721 gatcatcaca gctgctaatt tatcgatgac tttttttcgt ttggctcttg aagtatttgc
    15781 ctgctctact agcatacgtt ctgcctgaac agtggttaat cgttcatctt gataatcaac
    15841 tggcaaggag aatagttctt tgatcttttc tccgtatgac atggaagctt ctgcccttgg
    15901 tccgatggaa ttgttcatat ttttaggtaa tccaactaca aaccggtcta cttcatattg
    15961 tgctactaat tccttcaacc gatcaaagcc aaactcttct tctgcttcat tgatccgaat
    16021 gatctctact ccttgagctg tccagccaaa ggggtcactg atagcaacac ctactgtctt
    16081 agaaccgaca tccaagccca ttactctcat aaattcactc catgatttga gaggtagtcc
    16141 ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc gaatcaaatt tcgagcatca
    16201 cgataacgag gaatgtaggc cgggtctcca gaaagcaagt agccgacaat ctgattgatt
    16261 ggattgtagc ctttttcttc caaagcccga taaacaatcg ctaatgtttc gctcacttct
    16321 tttttgcgac tatcgtcaaa accaaaacgt acggtttcat ctgtaaaacc catgattcta
    16381 cacctctttc ccgcaaggta attccagctt accttctaca accttatttt actcgaagaa
    16441 agggaaaact acaaacaaat attccaaact tccgaataaa ttaagaaaca ctatttgtat
    16501 gtttttttcc ttttttctgt agctttatca aaagtactct tttcataaag tatacgctat
    16561 atttctttga atgtatgttt ttacacaatt ttttcataat attcataaaa aattcgaata
    16621 gctgctacta attttttgtt tttgttgctt gtcctgtctc ataattgatc gtgtagccat
    16681 cttgaacatt gtagataaag acattgaatt caattttttt gtcttcaatg ctttctgctt
    16741 ccaattgcac tcccctggct accagttcat tcccctcaaa atgaggagtg acacggtagc
    16801 gtacatgatg atttgtttct ttgatatatg ccgcaatatc attttcgtga gcaagcatgt
    16861 atggagcatt caacgaacgt gtccctgtca tcaagttttt aatgttgtta ttttgaccag
    16921 tcagctggaa accaattaaa tggctgcgat tatataacca ttgcccgtca cttaatttct
    16981 tttgtttcca acctgtcggg tcaatataca aaggctctct cttttcagta gggaacaaat
    17041 cttttccaag catagcattg gcttgtccta ctctgttcaa tttatcaata tttgaatagc
    17101 tttcccatgt tcctttggaa aggctaagat cctcttttgt aaattccggc tgattcccat
    17161 tgatttctat ttcttgatgt tttccatcat attctggcac atccgctact tgagaagctt
    17221 gtggtgtgga atcaaaaata gttgtaatct gctgaaagat atctgaatct ttttgtgtgg
    17281 tggaaacacc gaatgcccct gcaatcacag cagcgacgat ccccacgatc gccttggttg
    17341 tttttttgct catttcttta ctcctttact aacagctccc taaatcttaa catatgttcc
    17401 cctataaata aatactagaa agaaatagaa acagcaaaaa ataaaacctg ttttaagatg
    17461 gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa tcagtcacca ctctatctgt
    17521 aaggtattta ttattattca aaaattttct tcatactcaa gacaatacac tgatttcaca
    17581 accgcttggt aaacggtgcg caaaagcttg catctgcggt aataagtcca tccaagttaa
    17641 aaacataaga agttgttttt gcttggctgt tcttattact tgttgcagag cagctttttc
    17701 acaacctatt ttaagcgggt gacgggaatc gaacccgcga catcagcttg ggaagctggg
    17761 attttaccac taaactacac ccgcaataca aaaacagtat acgcctcttt tattccttcc
    17821 tcaagttttt ttattcttta cgcttctggt atgttttgta gtaaaataaa actaatcaat
    17881 caattattgg agaagatgct atgacgggaa aagaattaga tctgttgctg gacacttgtt
    17941 tactcgctgg gaaaatcatg atggaaagca atgcggagat gtatcgggta gaagatacga
    18001 tgagtcgaat tgctttagct tctggaaatt tccgtttggt cagctatgtc actcaaaccg
    18061 gtctctttat tggtcttgat cgtacctcga ccatccgaat ggaacaaatt accaaccgtt
    18121 cgattaattt agaaaaagta gtaaaagtaa atgatctttc acgtaaatat gttgcttcag
    18181 agctaacatt agaagaactt tactgcgaac tgcaagcaat cgaccgagac cgttctttct
    18241 ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag tggaacaatt atggtgttat
    18301 ttggcggaaa gatgaccgat ttacctgtga ctttagtcat tggaggattg ggatacgcag
    18361 cttatctata tagtttgaaa ttttttagaa tcaaattttt atcggaattt ttatcttctt
    18421 tattgattgg catagccgct atctacagcg tacgttttgg cttaggaagc aatcaggatc
    18481 taatcattat cggatgtgtg atgcctttag ttcctggagt tcaaattacg aacgctatcc
    18541 gtgatttgct agctggacat tatgtctcag gagtttctcg cggaacagaa gcgatgatga
    18601 ctgctacgat gatcggtttt gcgattgctt ttattttcca gcttttttat taggaaagga
    18661 acacaagtat ggggacgata tttattcagt tttcttttag ttttttagcg actgcggcat
    18721 ttgccgttat tacaaatgta cctaggcgtt cattgatttg ttgtggtctt tctggtgctt
    18781 ttggctggat gatctactgg atctgtgttc aaataggcgg atctgctgct tttggttcat
    18841 tgcttggttc tttaggagta gcattcatca gcgatctttt ctctaaacgt ttgaaaatgc
    18901 ccgtaacgat tttcaatatt cccgggatgg ttcctctagt ccctggtggt ttagcttatc
    18961 aagcggttcg aaatctcgtt actgaatcct atcgagaagc ggctcattat gcggtccaag
    19021 caattatgat tgctggagca atcgctttag gtcttgtatt atcagaagtc tttaaccata
    19081 acatccgtaa tttcaaagta aagcgtgacg caatcatgat gaaacgaaaa aaacggcttt
    19141 ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga gacgttcagc cttgagcgat
    19201 gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg agcaataaga agtaattatg
    19261 agtattaaaa aaagttttgt aaaaatagct tctcacattt ttacataaaa ttgacttaat
    19321 actgagaaac tgacttttga acaccctcta tctgtaaaaa aggggctgcg acatgatttt
    19381 tgtcacggcc cctcttcttt attcaaattt tttccaaaac gttcgttgat aataaacgat
    19441 ccatagagcg gaaagtatta gaactaaacc gatcacagga atattcacta accaaaatgg
    19501 ataaaacaaa gccaacattc cgtataagac gataagaata atactgatga tcaatgaacc
    19561 catcatagtt gcgactaaac ccaaacttcc tgaacctctg ttaaataatt ggttaatatt
    19621 ggtccagttc agcagcagca accgataatc tctagcgaaa taacgtagtg acaataagta
    19681 gcagccgaat aaagaaccaa atagcaagct agggataaaa aacaaaggca gctgaaaaga
    19741 cagtccgctt aataatgcga tcccaccagt taaaaaactc tgcaatatcc atccgaatct
    19801 gaacttttct tttaaatatt ggttcataga gattggcaaa gaacggataa acagaaaatt
    19861 ttcttgatcc aaagaaataa gattgctgat aaaagaagtc tgattaactg ttaagaaagc
    19921 caatgcgatc cctaccagga aaacaacacc gattaactta ttatctaaat cagttaattt
    19981 tatttcgcct cctagggcaa aagcaagaat aaaaatgatt ggagttaaca aggaagaaga
    20041 aagaacttgc ataatcaaat taggttcttt tactaattga gcattgtaac taaataaaag
    20101 ctgatgcaca ttttggttct ttttatgggt tctttttatt ttgaactcac tgtttgaatc
    20161 agtcagttgc tctgtaagct ttggtactat cagctgtttt acagcaagga aaaatcccag
    20221 agccgtgata gctagtattc cccaacccaa taaaccttgt tgagaaagtg gctgactggc
    20281 agcatcatag aatggcaata atatcgaaat cggatgccga tctgttaact cattttcaat
    20341 agtccccgtt tgatgattca tccacatgat tccagcaaat gcgatcagca tcgagctgcc
    20401 aagcaagata ctagaaactg tttttctatg ttttttaaac actgctgttc gagccaaacc
    20461 aaaaacaatc aaactagcca gacaaaaaag aataatcaaa aatagagaaa acaaaaggat
    20521 tgagataatg atagtaagca aaatccctag atcagctcgc catccggtta aaataaacat
    20581 gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca actaccaaaa tctttgctga
    20641 aaaaacctgt tcttgtccaa aaggcagagg caaaaatact tgcaggtcat ttccttcaaa
    20701 aaagatatta taaatgactg atatactttg agttaaacct aacataccaa aaagtgccac
    20761 gtaataagta aagaaacctg gcaatttaac aaagttcata gcaaacatag caaatccgta
    20821 aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac aaaagataga tagttaatcg
    20881 ctttcccttt tttcctttct ctcttgcttt attcgtgagc tgtggattaa catataaaag
    20941 attaactcga attaattcta ataattgtcg cttatacata cggtccatct cctgtcgctt
    21001 gccttccagc cattttcaaa taaatctttt ctagcgtttc atcaggtgag ccattcaata
    21061 attcactaac tgccccctga taaatcaatt cacctttctt caaaatagcc agtttatcgc
    21121 ataactgttg tgcggtatcc aatacatgtg tagaaaaaat gactgttttt ccttttgcgg
    21181 catgagcctt catcatttct ttcaaatcaa atgctgcttg aggatcaagt ccttgcaaag
    21241 gttcatccaa tacccaaata tctggatctg gcagcaacgt gccgatcaaa atcgtttttt
    21301 gacgcattcc atgtgaaaaa cttgcgattg tttcattttg atgactatac atatcaaaaa
    21361 gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt gtctaattca taggcagctg
    21421 aaatcaagtc ccaatattct ccagccgtca attgtaaaaa aatatctggg gaatcaggca
    21481 cataaccgat tttttgtttg atactcaatc gattttccgt caatttcatt ccatctacta
    21541 aaatcgttcc cgatgatggt tcaataatac taactaaact tttaatcgta gttgattttc
    21601 cggcaccatt atgtcctaaa aatccaaaaa tttccccttg tcttatcgag agagacaagt
    21661 cttttaatgc ttgttttttc ccgtaacttt tacttacatt gatgaattca atcatttctg
    21721 tactcctttt gtttgttatt actttttatt gtacccttcg ttattggtgg agtaaatgtc
    21781 aaaaagagtg cataacaaaa aaggatagaa acgactaggc tgctacttgg gttgttctct
    21841 tctacaggaa aacactcttc taacaggtaa gatatgagtc tttgttttct ttaaaatgat
    21901 tcattttcta tcttttcttg cataaaattc tttagtttat caatacttct ctttcccatc
    21961 tctagttgtt ctccatttgc aaatgttact atttcgttta ctctgctaat agatgtaatg
    22021 ttttcagtat taatgatata agcttttaaa tctttacata aatactcata ttcgagtgat
    22081 tttttatatt ttttaattgt gccgtttacg agaatctctt catctttcaa tactaggcct
    22141 attttatttt tataacctgg catgctcatt agataaagga tttcatcgta atggatatag
    22201 tgatcgctat taccataacg gacagttgtc cacctttttt tcgtagcatg ttgaacagga
    22261 tagttttctt tttctttcaa taagtaactt tggatgcttt ttttattttt ttcataagtg
    22321 aattctttta tcaaataacc actaggcgta atctttcgat taataacagt tattgcttta
    22381 ttcggatgag acgttagata aacaatttta cacttttcat tcttttctct aagcatttcg
    22441 cctagttcga taccgctata ataggcgttc aaatcgatgt ctaggaaaaa aatatctgag
    22501 gaatagattt cattattttt aatgaattca acaaattgta taacgttcaa tccctgaacg
    22561 atctcaaatt tccaaggact taataaaatt ctggattctt ccaaaatttc tactagcatt
    22621 ttttcaaaaa ccaaactatc atccaaaata aatatacgcg ttaccataaa aagtacctcc
    22681 actcgatatt ttactatcaa aatcatttca tacaactttt caacatacat gttaggaata
    22741 aattgtaatt ctcttcacaa tcttttccaa cacctttgaa accgatatca aatactttca
    22801 ggatgctgct tctaaaataa atatcaaaca aatgaataca tcatgcaaaa aaagccagac
    22861 aaccgacgtc cttgcgtgtc agctgtctga gctatgttta tttattttgt tcttttaaaa
    22921 agaacgatac acatactgat ttttcggagc agagactttc tcagcgtttg taacaactac
    22981 agatgggatt tcacgcttga atggttgata atagtctgat tggatggttc cttctatttt
    23041 ataccattca tcatttttaa ccgttactcc ttccggcata tgcgtcaaca aaccaaagac
    23101 tcctgaatct gccacacaat ggataatgcc gaatcggaac aagaagatat ctgtattgct
    23161 atcgttcggt gtttggtaaa caaatccttc atagctgatt tttttgccaa tgaattcgct
    23221 agggtaatta taaattagct ccataacttc taaatagttt tcatctgtga tcttgatagt
    23281 ttgcttatcc ccatactttt ctttcaactc attcatttgg tctaagtagt ctgatttgtt
    23341 gaaataaata ctggtatccg gctttaagta ttgtgtctgc atctccggat cccctactga
    23401 ttctttactt actgggaaat taaatccttt ggcttctacg atcgtagtgt cgagactaac
    23461 tgttggaaac aacgtcccca cgattactgg caaagctagc agtacataag cgattccacg
    23521 ttgataaggt tttgacaacc catggtcatg atcgtgctcg tgtgcctttt cttctgcttt
    23581 tccgtttttg acccacaaat acagctgaac taaagccaaa agagcagata aaatcattga
    23641 aagaatagct aagtagcgat aatggacatt gatatattga tcgagtttgc cagaaacttg
    23701 caagtacatc atcaattcga aatacccaga taaaatcaaa aaccgcagca tattttccct
    23761 cctaaataaa taatgtgaat ccaataacgc taagggcaat cagtccgatc agcgataaaa
    23821 taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt tttgatatcg accattgggc
    23881 caaagaccaa aaaggcagct acaggtgctg tgccgaacag acttaacaaa gaagatccta
    23941 taaacgcatc tgcttctgag caaagagaca tcgtaaaggc caataacagc atgatgataa
    24001 tggctaaaat ctttgttgag ccgagcgtta gcataattct agttggtaaa taagtctgca
    24061 tcgcagctgc gattaatccg ccaatgatca aataacgacc tgtatcaaaa aattcgtcga
    24121 ttgaatgtgt caacacatgc ccagtttgat ggatcagctg attttttggt tcattcttct
    24181 cactttgttc ttcttcatgg acatgatgat ggtgattttc tgctacttta gacgtgattt
    24241 tttctttcaa tacagcgtct ttttgaaaat aggcaagcca gacaccaatc acaagggcaa
    24301 caaaaaagct gcctatcacc cgccagcttg ccatctgcca agaattgcca aatgcgataa
    24361 aagtggaaaa aatcacgatt ggattgatga tcggagcagt cagcataaaa gcaaacgctg
    24421 tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat cggtacgatg ccgcattcac
    24481 atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat agagaagata cgattttcag
    24541 gaagccatct cttcacacgt tctggggtca aaaatacttg taaggcgcct gagataagac
    24601 agcctaataa tacgaaaggt aatgcttcaa taacgatcga taagaaaatc gtactcatct
    24661 gcaaaaccga atttggaata aaagaaaaca tgagtttcct cctttttatt tctagttcat
    24721 tcgctttgca aatatacgct tttattcaaa aaaaacaagt tgctctgtaa atttttacac
    24781 aactcttatt ttttaccaaa gcacttcaat tgactttttc tgttattccc attaaattaa
    24841 ggtctcatac atatcacttc tatcataaaa gggagaataa aaatgaaaca aaccactaac
    24901 ttgactgaag ttcaagacat actgcaacag tcatccgttg ccgtcattta tctttcgatg
    24961 cctaattgca gtgtttgtca cgctgttcgt ccacgactag aggaacttct cacacattat
    25021 gatattccgg cacttcattt ggatgcattt gaaacaccag aagtagctag taaatttgaa
    25081 gtgctgactg cgccagttgt cctgattttt catcaaggaa aagaagtctt tcggcaagca
    25141 cggttcatcg actttaaaaa aatccgttat ctactagatg aactgacagc agaagatgat
    25201 tccctttctt atgaagaaat cttccgaaca aaataatcgt tgtccaaaca gaaaaaagcg
    25261 attgaacaag tgaaagtttt gccttcattt gttcaatcgc tttttatttt tattctgctg
    25321 tattttcgga atgcgcattt gcgtcagaat gcaagaaata gatcaaggtt tgcaattcat
    25381 tcgtcaaatc aacattttgg atcaatacat ctgacggagc attcaatcga gcagcggtaa
    25441 agttcaagat tcctttgatt cctgcttccg ttaactgatt gactacttct tgtgctcttt
    25501 cagcgggtat cgttaaaatt gctacttcga tttgttggat cttgatttgt tctgacatat
    25561 cttccattgg ataaacgggg ataccgtcta cgatgcgccc aacgatttcc tcattcacat
    25621 caaaagcaca actgatacgt atgctattac tttgatggaa tttgtatttt agtaaagcac
    25681 ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa ttgatcgtcg tttaatgtct
    25741 ttgcaaaaaa gtgcataaga tcttctacat cataaccata cccacgtttt cctaattcgc
    25801 caaaataaga aaaatctcga cggatcgttg cactatctac ttgaacggct tcacttaatt
    25861 cagttgaaga cacttttttc tttccagcat tatttaaaat ccttaaatag cgataataca
    25921 aaggcagtct tttagctgtc gcctttggaa taatatgatc tttcacgaaa tgacctccac
    25981 gtattgtcta attacatctt tgatttctcc atcatagcag tatttatagg aaaaaatcaa
    26041 ttccattgct cagatattgt gaaattttat tctttattca tttatttatc atttctgcaa
    26101 atctcgcagt ataatggaat ttatggtaga ctactaattg aaaaatagtt ttttatttga
    26161 ggagaattca caaagtagaa aagaggattc catgatatta ttacaagcca atcagattgc
    26221 tcgccatttt ggtgcagaag tcttatttga aaacatacat ttagaaatca gtacaggcgc
    26281 tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg actttattaa agatcattgc
    26341 tggaattgaa gcacctgatg aaggaaagat cgctaaaaat aaaacagcga ctctcggata
    26401 cctcgctcag gacacaggac ttgattccaa tgaaacagtt tggaacgaga tgctgaaagc
    26461 ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag ctagagattt tcattagcga
    26521 aggtacgcca gacacacctg cttatagttc ttatttaaaa gaatacgacc ggctgcaaca
    26581 cgatttttca gatcgaaacg gctatggtta tgaaaatgaa attcgttccg tattgcatgg
    26641 gtttaaattc gattcttcat tttatgatca gaaaatcgat actttatcag gcggacagcg
    26701 cacacgttta gctttagctc gcatgctttt gcaacaccct gacattttga ttctagatga
    26761 accaacgaac catttagata tcgagacact ttcctggctt gagaattatc tgcaaagcta
    26821 tactggtgca ttattgattg tttcccatga ccgttatttt ttagataaag tagtcaatga
    26881 agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa ggtaactatt cgaaatatct
    26941 agatttgaaa gcagaacagt tagcaagtga atggaaggct tatgaaaagc agcaaacaga
    27001 aatccataaa ttagaagact tcgttgctcg gaaccttgtt cgcgcctcta ccacaaaacg
    27061 agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg ctggatcgtc ctcaagggga
    27121 tgaaaaacaa gcaaactttc tattcagtat agaaaagcct tctggaaatg tagtcttgca
    27181 aatcgaagat gccgctatcg gatatgataa tgtcacctta tctgaaccaa tcaatctaga
    27241 tatccgtcgt caagaagcga ttgctctagt aggtccgaat ggtatcggga aatcgacgtt
    27301 gctgaaatca atcattcacc agatcccttt tatccgtgga aaagaaacgc tagggacaaa
    27361 tgtcaccatc ggctattacg atcaaggtca agctgatctg catacaaaca agacgatcct
    27421 agcagaattg tgggatgagc atcctaccac tccagaaaaa gagatccgaa gtgctttagg
    27481 tgctttctta ttttctggcg aagatgtaga aaaaccaatc cctctgctaa gtggcggtga
    27541 aaaagcacgg gttgctttag ccaagttagc gatgaacaaa gaaaattttt tgattttgga
    27601 tgaaccaaca aaccatttag atattgacaa caaagaagta ttagaaaatg cattgatcga
    27661 ttatgacggc acactgcttt ttgtttccca tgaccgatat ttcatcaatc ggatcgcgac
    27721 aaaagtcgtc gaactctctg aaaatggcag caaagtctat ctaggagact atgattatta
    27781 tttagagaaa aagcaggaag aagaagaaat cgccgaattg ttggcacaag atgagccaga
    27841 atctgctcct gctcccacga acaaatctga ttattaccaa agcaaagaac agcaaaagct
    27901 tatccgtagc cttcaacgaa aaattactca ggttgaagaa gaaatggcgc ggatcgatga
    27961 atggatcgat cagttggaac aagaaatggt cgcgccagaa aacctggaag atcacgtaaa
    28021 actcaatgag ctgaaccaag atttagaagc tgctagacaa gatcaagaaa caaaattgac
    28081 ggaatgggaa gaactaagct tggaattaga agaatatgaa aattaacgaa aaaaccaaaa
    28141 cagaaaccat tcatgctgtt ttggtttttc tttttttatt ttatcaaaca aaaattccat
    28201 atttaccttt ttcaccaata agaagaccaa ttactagtca ttctcactaa agcttctaaa
    28261 taatagtaat caccccaaat acaaggtgta tttaccccat tatttttggg cttattataa
    28321 acagactgtg ttaaaaagcc atcttcttct tccgttgtag catatctttc tttcaattgt
    28381 cgcataattt tccaaacagc agactggtat aagttctttt ctgttttgtc taatagacct
    28441 gattctatta attctaataa tccacaaacc atgatcacca tactagatgt atctttttct
    28501 tctctgctag aatcatcaaa gatcaaatcc caataacaaa tggtatcttc tggcaaccga
    28561 ttcataaaat aattggctaa tattttgctt aaatcaaaat atttttgttc tttcaaataa
    28621 cgataactta atgcaaaacc gtaaatcccc catgcttggc ctctagccca acaggaatta
    28681 tcactataac cttgatgcgt acttccatat cttggttttc catctttgac atccatataa
    28741 aatgtatgat aagttgaacc atcttctctt actaaataat ctgctgcatt ttgaatatga
    28801 gcctgagcag cttttttata tttttgattt ccagtttctt cactcgccca atacaataat
    28861 ggaagattca tgcaacaatc catgatcatt ctaccttgtt gtgaaggatc actaggatct
    28921 ccccaagctt gtataactcc tgccttctcc caaaaacgct gaagtaatgt ttctgcagca
    28981 tcaattgaag cttccttcat ttttcgattt cccgttattt tataatctgc tacggttgat
    29041 aaagaataca aaaaaccaag atcgtgatga ttagtgataa tttgacagtc aatcctgtgg
    29101 ataaaatcta tatctgcttt ttcagctgtt tgttgaaata aaggatcacc cgtcttctca
    29161 aataacaacc aattaattcc tgtccaaaat ccttctgtcc aaccaatatt gtctcttaat
    29221 ggataacgtc cttttatagg acactcgctt gggaattgat actgactgtt actgatacgt
    29281 ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct cattattcca ttctttttcc
    29341 attaattctt tataacattc atttatccat ttttctggat gtattatctc ttcaaacatc
    29401 actttatcaa gcatttcccc accagccttt agcataattt tagtcttttt ttattgtttt
    29461 tattttggat agttagcccg gttgtcccat caaaatcaat tttgggacga ttgttccata
    29521 cttctaaact ataatcccga tcttgatgag caaccactcc tgttccgagc caatgggttc
    29581 cttttccaaa tgtaccctct aatccaggaa taaaagtgtt ttcccatgtc atcaagtttg
    29641 tattgggcaa acccataata gctgtggatt ttcgtttaat agtatcgtca cttaaacaga
    29701 taatacctga aaagccagct tcactcaccc tatagttttc cttgtcttct tctatgatta
    29761 ttccttcttt ctcaggagaa cattcaatag caaaccctgt ctcatataga acatattctg
    29821 tatctaattc tactttgtgt acgcgaatct gccagccttc aaaaggaata agccaagttc
    29881 tgatttttgc ctgtaaagta ggttcccatg tagaggcaag tacctctttc ttacatacaa
    29941 tgtctcgtac attatttcga acgatgaact gtccataggt tttgatgctc tcattcttaa
    30001 aactggtcgt gatatatgtg tccgctcgct gaatccctag cattgaatcc attgccattt
    30061 ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa ttgactagaa taagcaaatt
    30121 ttaaatattt ttcattggtc aatgtatgga aatttggccc taattgtccc gcattcaaaa
    30181 aaaatgtatg tccattatca tgttgtatga gcaaattggc ttctgaaaga agtttagttc
    30241 cttcacgaat aggcataggc atttctttag ccgtccaata ctcatggtct tcaggtagcg
    30301 ccaacaataa aaaaatctta ttcagccata atggagataa cgtactatta tagggttcag
    30361 tcaatgtcaa ttgagggtag gtataaccaa tattcaggat acctttttca tcaaatattt
    30421 ctttttgcat ccaccatctt atattccgat tgactaaccc cttgatttca cctaatttaa
    30481 aaggcaaaag atcggcccac aacatagctg accaaaagct tgaaactgca aaacgataaa
    30541 tcatgcttcg accaaaaggc acatttgcac catcttccgc aaaaaaatga atatagtctc
    30601 tagaaaaatc aatagctcgg ttcttatata tctggctaat gggcttttct ggttctagta
    30661 cgctataaat caaaccataa taatgaaatg caaaaggatt ataataatct tggcgacctc
    30721 ttgaactgtc tcgataccag ccattttgaa gataacaatt ttctatatta tcaaatgctt
    30781 ctttcagtgc tgattcttta actggataac caaatttttg gataattgtt ccgacaatta
    30841 tcttaaaaaa ttgccaatta ccatctgcac attcatattc aaaaatttgc gagaaccaat
    30901 gaataatctg gcttttttct cctgtatcta attttttcca taataattgt tcatgatgat
    30961 ataacgctaa taacattgcg ggcatttcta ccatacgctg gtctctttca tgtatttctc
    31021 cccaataatc gggatgggaa ggatctgttc cagcttttat tcctgataga attaatttca
    31081 accatcgttc atctaattgg tcaattgcgg gccccgctcc ccataaaacc cgagagaatc
    31141 cttccattcc tgcaatttta tttccatatc ctaccccttg atgtatataa tcaatttgtg
    31201 cgccacttgc tcgataaaac ggctggagtg gttgatttat ctttttaaat aagtcaaagt
    31261 aatcctgtct ggtccttaat agtggtactg gatgtttcat tgtattttct cctcttttta
    31321 ctaccaaata gctaattccg actgttctaa tcgaagtaag ccttcaataa aaaaataatc
    31381 agaatagatc agtggcacat tataagtcag ttctctatgg tatgcttctg ctccgcctcc
    31441 aatgatccca tcttcatcca tattccaatt tgcatagtgc ttttctgtct tttggagtat
    31501 attgatagca gcttcatgat acatttttga tcgtacagag gaaacatatc ctgctaattc
    31561 cagaagaccg catgcagcgc atactccagc actggtatca atattcgaat cttttacagc
    31621 aaaatcaatg agaggaatat aaccagtttt ttcgattttc tgaataaaat ttactgctat
    31681 cttttctgct atagctagat agtacggatt ttttgtgtaa cgataactta acgcgaatcc
    31741 atataaagcc catgcatttc ctctgctcca acaagaattc tcattgtatc cttgcccgcc
    31801 aagtaaccga atatatttcc ctgattctgc atcaaattct gcgatatgcc cggttgatcc
    31861 atctttcctc actagatact tagctgttgt atctgcatgc tgccgagcaa ttttagagaa
    31921 cctcggatca ccagtttccc tcgaagccca atacagtaat ggtaaattca tcatactatc
    31981 aataatcatc caaccaggtt tatggtcatt ccatgctact aaatagttcc ctgaaatatt
    32041 atagcgtcca gcaagtaccg tagctgcatg taaccctagt tgtcgactat gtttgttttg
    32101 agactgttga taatgtgcaa tagccgatgg taagaataaa aaacctacat catgatgaac
    32161 gtctgaaaaa tggtacaggg cagattctaa taattgttct tgttgtattg ctgcctcttt
    32221 gaacttttct cgttttgtct tgtgaaagag ctgccataaa ataccactcc aaaaaccatt
    32281 ggtccaccag tcgattcctc tgtcctttat tacgtctgta tattttccgt tgtaagaaat
    32341 atatggaatt ttattcccca ttctgtctaa ttccgcatct atcttttcag aagctacatt
    32401 caaaattttt cttgaccatt ccttcgtctg ttcattcatg gatatcccgc cattctttta
    32461 atttttatga atgtggagtt gctcctagaa cgaactagat ggttaacatt cgttattttt
    32521 tttacttatt tgcagctcca atgtcattct ccttaaattt agactgcgcc cacacataaa
    32581 tcaagaccat tgggatcact gaagttgcta aaattagcat aattgcgcca taattaattg
    32641 gctcacctgt actagaaacc gtcatatttt tgataattaa gggaaccgta tatttatctt
    32701 tatctgaaag catgactaat ggaaatagat attgattcca acttcccata aagataagta
    32761 aaccactagt aataatcgta ggtgataaaa tcggtacagc gattttcgta aaaatcaccc
    32821 actcattggc accatcgatt ctggcagcct ccatcagtgc tgtaggaaat tgactgctat
    32881 attgtcgcat cataaacact gaagttgtac tggcaatcga agggatgatc acacctgcat
    32941 aagtattcga taatcctaaa cgactcataa tgtaaaataa aggaatcaat gtggtaaaaa
    33001 aaggcaccat cctagatagc ataactgtat tgaacaacag tttattcccc ataaaacgat
    33061 actttgctaa tgcataaccc gccattgtag aactggctgt agctaaaact gtactgatta
    33121 cagcaataac aatcgaattc caaataatca agggataatt atatctcgcc atgatggctt
    33181 gccagttttt taatagcata tccccaaact tgaaattgat tccgccactt aaaatttctt
    33241 gattactttg cgttgaagct acaatcatat ataaatatgg catcaaaaat attaaggcac
    33301 caaacacaat aatgacgtac gtaaaaattc tgctttgtat cttttgttta gtcattttta
    33361 tcacctccga atttaaattg aatagccgat aatataacag atacaaacac catcgtccag
    33421 gcaatagcag aggcttttcc aaaatcaacc atttcaaaac ttgttttata taaattgact
    33481 cctacgacca aagctgaatt ttctggtcca ttggaagtcc caaagaatat atttgggata
    33541 ttgaaattac ccaagccatt gatagtggct aaaactgtgg ccattaaaat gattggcttt
    33601 agcatgggga tcgttaataa aaagaattgc cgaaatttcg atgctccatc tatttcagca
    33661 ctttcgtata tctctttaga aatcccttga agaccagcta aaaataaaat agtgttatat
    33721 cctacactaa gccaaatcat cgtgatgatc attgatattc gtatccaaaa aggatttgtt
    33781 aaccactgta caccgttaat tcctagcgca ttcaaaaatc cattgaataa cccagtcgga
    33841 ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg atgtaatggt tggaatataa
    33901 tagacagttc taaaaaatcc ttttcctttt aactgtgctg aattcaataa aacagcaaag
    33961 taaatagcta atagcatcgt tattggaatt gatcctgcca tgataatcaa aacattcgtg
    34021 taagatttaa taaacaatgg atctcggaag atagctagat aattgtcaaa tccaacaaat
    34081 tcaaatgagt agccaatttt cataaaacta aaagcaaacg agataatgat gggaatcaaa
    34141 atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc cataagcact ttttttatga
    34201 gacgtatctg ttgctttttt cttgctgttc tttattgtct tgcttggtaa cgaagattgc
    34261 atcatcgtta agtcctccta atatttttta atcaccagtt aattttacgc cgtacttatg
    34321 agcaaaattt tctgcttctt ttagatatga attttcaaaa tcacctgttt gccaatagtt
    34381 atatgcagcg gtagtcaaat aagacgtagc atctgaataa gtggctggta aaacagtatt
    34441 tgatgtattt tcattgattt tattcaatag ttcatagtat ttttgtcctc cttagtagtc
    34501 aaactttttc tcagctgctt ctgtttgata ggccaatgag ttagatgtag ccacgcccag
    34561 ctccagtgcg ttggctaagc attcttcatc tgtcagcata aatgtaagaa attgttgtgc
    34621 cacttgtgga tttttggcat ctttagggac ataaaaactt gatcctcctg atactgcacc
    34681 ttttcctgga ttttcttttg aaaaaggaat cagtggagcc atgcgccatt tgtcagatgc
    34741 atcaggaaaa ttcaaagaca tattcgtagc ataccagccg ccagcaacaa acattgcaga
    34801 ttcttgaaat gctttctcag catcttgtgc accgtagaaa tgtacaatat ctgagtcgat
    34861 catttctttg attaactttg cagcatgttt cgcttcttta gtattcagac ggatatttcc
    34921 ttcttcatca agcaaagatt gattctgctg ggctagcatg ttgataaaca attcttggtc
    34981 cccagattct ggcaaggcaa ttccaaaact accagttttt tcttttaatg cttttgccac
    35041 ctggatatat tcttcccaag aactgatttc ttcaaaatct acacctattt cagcaaattt
    35101 ttttggttga taataacaaa gtgctggggt aaaatcacta gcccaaggaa taatcgtttc
    35161 attactcgtt tgttgacgca aaatattcaa tttttgatca taaaactgat cgccatattt
    35221 gtcataaaat ccatattcat cagcactata aaaagcatct tgaaattttt ccagcacacc
    35281 tgctgctgaa gtgtctgcca tggagataat atccggcata tcttcgtgtg ttaccaattt
    35341 tggggtgatc atttgtactg ttgctgttcc cgaagctaca ttcacaattt ttaagtcaat
    35401 atttgctcca gttttgcggt tatagatttt tgcggcattt tcaacatagt ttgtcagttt
    35461 gccgtttgcg acatatactt ctaatgtagt ggcttttttt atcattggat ctgcgatgtt
    35521 tgctgttact tcttcgttag tttttccaca tgcacttaag ctgaataata gggtaatgtt
    35581 tagcaaagct atcaccaatt tgtatctttt tttcatgtct atcccttctt tcttttcaag
    35641 ttcttatata aaacgctttc acttatgtat attaacggaa ttagtaacac aaatatttat
    35701 ataaaaaaat attttacttg tcgttttggc ttttcatttt tgttataata gtcattaatt
    35761 ataaatagaa agctttttat ataaaaagag aaaggaaagt ggttcttttg ttaacaaaca
    35821 accaatcctc caatcttgtc aaaaaaacac taacctattc catagatacc catcttcatt
    35881 tatctgctgc tccctatttt cgactactaa taaatgaaag cgctaattta tatgctgaat
    35941 ttgcacataa atctatagaa ctagataatc cttccctttt attcattgat aaacagcaag
    36001 aagtccaaat cttttctact caaaaggaaa cacaaacttt tatcattatc cagttttatt
    36061 ttgatgagca attattagca catattacgg gacaatcttt ttctatagat caagaaataa
    36121 catctatcat tacttttctg aaaaatgaac agttcaaaat tgattatcta aaacatagta
    36181 agccagctat ttcctatgat caggaaatca attctttttt catactatgc caacagatga
    36241 gcattcattt gctttcctta ttagttactt attgtctgca aaaaattcaa ttgtcttcct
    36301 taccaaggca ttatcaacag gaacttgctt tattgacaat accttcaaat gagaaagtca
    36361 ttcctaaaaa aaagcatatt ctttattcag atgaattagt tgatccaatc atacgatatt
    36421 tagaagaaca tctttcagaa aattttttgc ttgaacaatt agcccaaatt ttttttatca
    36481 gtccagccag cttaaaaaaa cattttagaa aagtgaatgg aaaaagtgtt attagctatt
    36541 ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct agaaaatgaa ttaacctata
    36601 cagaaattgc agatatatta ggatttcatt cgatccatca tttttcttca gcttttaaaa
    36661 aatatacagg gttatcccct tcaaagtatt atctatcctt aaaatagaaa aaaattttta
    36721 ggttttatcc acaaattatt tctctatatt ttctgtctat tcaaaataaa caataatttt
    36781 ccggcacaat tatttgctgt tttttaccat aacatagtac gatcaacgta gaaaaggaga
    36841 tgtttttatg aaagttgttg tcattggcgg tgtagctggc ggtccttcat ttgccactcg
    36901 tttccgtcga ttgaatgaag cgcacgaaat cattatctat gaacgcggag aaaatatttc
    36961 ttatgcaagt tgtgctttgc cttattattt aggaggtgtg atcactgacc gtgactcgct
    37021 gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac atcgatgtct ttactaaaca
    37081 cgaagtaaca gcaatcgatc cttctactaa gcgattaaca gttaaagacc tatccacaaa
    37141 tgaagaaata aaaacagatt acgataagtt gatcatctct tctggtgcta gaccagatta
    37201 tccggatatt cccggagttt ttgaagcaga aaacggcttt gtcctccgta gtgtgacgga
    37261 tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca caacatgtcg tcattcttgg
    37321 tgcaggtgtt atgggtctgg aattagctga gaatttcaag catcgcggct tagacgtgac
    37381 tttaatcgat caattgccac aagtcgcttt cccttatgat ccagaaattg ctaatttagt
    37441 ttatgacaaa ttgctgaaag aaggattagc cgttcattta gaaacaagag ttactgagat
    37501 ccgtgataaa ggtcgagaaa tagtattatc agatggctcc gtcctttctg ctgatatgct
    37561 aatttttgct gttggtgttt ctccgaagaa tgaagtggtg aaagcaaccg gcatacaatt
    37621 atcagataca ggtcagatca ttgttgatga ccagttacaa accaatctgc cggacatcta
    37681 tgcgattggc gatattatcg aaacgactag tgtagtgact ggtcagccga tccaaagtat
    37741 gctttccagt gcggccaatc gtcaaggaca tatgctggca gatattttaa atggtacgcc
    37801 tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa atctttgatc atacagcaag
    37861 ttatgcggga atgacagaac atgaattaaa agcatcaggc ataacaaatt ataaaactgt
    37921 ttttatcact ccctttgacc atgcctattt ctatccagaa gctacaagat taaatctaaa
    37981 gctgattttt gatgcagata gcggtcgtat tttaggtgga caagcatttg gagaaaaagg
    38041 tgtcgataaa cggatgggag aactttctgt agcaatcacc ggaaacttga cagtctttga
    38101 tttgcctgat ttggagttgc cttactctcc accgtattct actacccgtg acccgttgaa
    38161 tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc gtagaaacga tcaaagcaag
    38221 tgatataccc gaaaacgatt tgaaagaagc gttcttttta gatatacgtg aacctaataa
    38281 agcaccttct ggcagtatct cggcaacaaa aaatattcca atgaatgagt tacgagatcg
    38341 aatcaatgaa atcccaaaag ataaaaaagt ttacattact ttcagaagag gattgaatac
    38401 ttatacttct gcccgaatct tggcaggttt gggtatcaaa gcgattttga ttgaagaata
    38461 aaaaaacgac cgcggtgggt agccgcggtc taggagttat ttttacttga ggagtaaaaa
    38521 tgaaaaagta tatagggttg ttggttggta tgtatataga atagaagata aatgtgaaga
    38581 aattgtgcat tatttcttca catttattag gttatagttt aagaaagttt aaacattgcg
    38641 caatttaatt attttagctg tttcttttca taaaaaaaca tgttgtgact ctgagacaaa
    38701 tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa tattacgagg catcatatta
    38761 atctatcagc actagtacct aaactagcat ccctttcgaa tattttttct tatataataa
    38821 agtttgcttt cactcctcta atctcaccaa atcgactggc ttgcgttgtg tgatactagg
    38881 aactgattgg tttagaacga cttttctatt gtaatcatat ttttcgccat cctcgttgtc
    38941 ttctaatcct tcaatgaatg atccctaatg tgtgtattaa ataatttaat ttttttgttc
    39001 ctatttattt aaca
//
LOCUS       DASOUD010000012        37454 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.012, whole genome shotgun sequence.
ACCESSION   DASOUD010000012 DASOUD010000000
VERSION     DASOUD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.012"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..420
                     /locus_tag="U4A29_000629"
     CDS             <1..420
                     /locus_tag="U4A29_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEO0679773.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="U4A29_000630"
     CDS             complement(504..1226)
                     /locus_tag="U4A29_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO0679774.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="U4A29_000631"
     CDS             complement(1216..1953)
                     /locus_tag="U4A29_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0679775.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="U4A29_000632"
     CDS             complement(1965..2957)
                     /locus_tag="U4A29_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO0679776.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="U4A29_000633"
     CDS             complement(2972..3715)
                     /locus_tag="U4A29_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0679777.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="U4A29_000634"
     CDS             complement(3956..5278)
                     /locus_tag="U4A29_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO0679778.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="U4A29_000635"
     CDS             complement(5265..6230)
                     /locus_tag="U4A29_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0679779.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="U4A29_000636"
     CDS             complement(6227..7765)
                     /locus_tag="U4A29_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO0679780.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="U4A29_000637"
     CDS             complement(8181..8468)
                     /locus_tag="U4A29_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679781.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="U4A29_000638"
     CDS             8602..9270
                     /locus_tag="U4A29_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO0679782.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="U4A29_000639"
     CDS             complement(9561..10301)
                     /locus_tag="U4A29_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679783.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="U4A29_000640"
     CDS             complement(10431..11378)
                     /locus_tag="U4A29_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0679784.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="U4A29_000641"
     CDS             complement(11507..12271)
                     /locus_tag="U4A29_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO0679785.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="U4A29_000642"
     CDS             complement(12275..12664)
                     /locus_tag="U4A29_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679786.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="U4A29_000643"
     CDS             12799..13341
                     /locus_tag="U4A29_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEO0679787.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="U4A29_000644"
     CDS             13489..14121
                     /locus_tag="U4A29_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679788.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="U4A29_000645"
     CDS             complement(14255..14380)
                     /locus_tag="U4A29_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0679789.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="U4A29_000646"
     CDS             complement(14514..15407)
                     /locus_tag="U4A29_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO0679790.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="U4A29_000647"
     CDS             complement(15589..16515)
                     /locus_tag="U4A29_000647"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO0679791.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="U4A29_000648"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="U4A29_000648"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO0679792.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="U4A29_000649"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="U4A29_000649"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO0679793.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="U4A29_000650"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="U4A29_000650"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO0679794.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="U4A29_000651"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="U4A29_000651"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO0679795.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="U4A29_000652"
     CDS             complement(24786..25220)
                     /locus_tag="U4A29_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO0679796.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="U4A29_000653"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="U4A29_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO0679797.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="U4A29_000654"
     CDS             complement(26117..26992)
                     /locus_tag="U4A29_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO0679798.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="U4A29_000655"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="U4A29_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO0679799.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="U4A29_000656"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="U4A29_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEO0679800.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="U4A29_000657"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="U4A29_000657"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEO0679801.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="U4A29_000658"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="U4A29_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEO0679802.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="U4A29_000659"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="U4A29_000659"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEO0679803.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="U4A29_000660"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="U4A29_000660"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO0679804.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="U4A29_000661"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="U4A29_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO0679805.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="U4A29_000662"
     CDS             complement(35443..36210)
                     /locus_tag="U4A29_000662"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO0679806.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="U4A29_000663"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="U4A29_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO0679807.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT        10192 a         7946 c         6425 g        12891 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgac
//
LOCUS       DASOUD010000013        36703 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.013, whole genome shotgun sequence.
ACCESSION   DASOUD010000013 DASOUD010000000
VERSION     DASOUD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.013"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            647..964
                     /locus_tag="U4A29_000664"
     CDS             647..964
                     /locus_tag="U4A29_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEO0679808.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="U4A29_000665"
     CDS             complement(1055..1420)
                     /locus_tag="U4A29_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679809.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="U4A29_000666"
     CDS             1628..2110
                     /locus_tag="U4A29_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679810.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /locus_tag="U4A29_000667"
     CDS             2307..3197
                     /locus_tag="U4A29_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO0679811.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3484..4758)
                     /locus_tag="U4A29_000668"
     CDS             complement(3484..4758)
                     /locus_tag="U4A29_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEO0679812.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5031..7502
                     /locus_tag="U4A29_000669"
     CDS             5031..7502
                     /locus_tag="U4A29_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEO0679813.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7605..9341)
                     /locus_tag="U4A29_000670"
     CDS             complement(7605..9341)
                     /locus_tag="U4A29_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEO0679814.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9338..10042)
                     /locus_tag="U4A29_000671"
     CDS             complement(9338..10042)
                     /locus_tag="U4A29_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0679815.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10173..10676
                     /locus_tag="U4A29_000672"
     CDS             10173..10676
                     /locus_tag="U4A29_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEO0679816.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10636..10974
                     /locus_tag="U4A29_000673"
     CDS             10636..10974
                     /locus_tag="U4A29_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO0679817.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10995..12413
                     /gene="ffh"
                     /locus_tag="U4A29_000674"
     CDS             10995..12413
                     /gene="ffh"
                     /locus_tag="U4A29_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO0679818.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12525..13103
                     /locus_tag="U4A29_000675"
     CDS             12525..13103
                     /locus_tag="U4A29_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO0679819.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13055..13627
                     /locus_tag="U4A29_000676"
     CDS             13055..13627
                     /locus_tag="U4A29_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO0679820.1"
                     /translation="MFPQRRGRTYRQKGRIKHAKIIFYGAISLDGYLATKTDDLQWLF
                     DTPTGKQTTYEAFYETIDTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPD
                     AEVVNEDPVAFLKNLQMTEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIP
                     LFQKGTYEARFEMLDTTRFGEFIELHYVRK"
     gene            complement(13706..14260)
                     /locus_tag="U4A29_000677"
     CDS             complement(13706..14260)
                     /locus_tag="U4A29_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO0679821.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14513..14788
                     /gene="rpsP"
                     /locus_tag="U4A29_000678"
     CDS             14513..14788
                     /gene="rpsP"
                     /locus_tag="U4A29_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO0679822.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14800..15051
                     /locus_tag="U4A29_000679"
     CDS             14800..15051
                     /locus_tag="U4A29_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO0679823.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15176..15967
                     /locus_tag="U4A29_000680"
     CDS             15176..15967
                     /locus_tag="U4A29_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679824.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16137..16658
                     /gene="rimM"
                     /locus_tag="U4A29_000681"
     CDS             16137..16658
                     /gene="rimM"
                     /locus_tag="U4A29_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO0679825.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16648..17409
                     /gene="trmD"
                     /locus_tag="U4A29_000682"
     CDS             16648..17409
                     /gene="trmD"
                     /locus_tag="U4A29_000682"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO0679826.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17545..17892
                     /gene="rplS"
                     /locus_tag="U4A29_000683"
     CDS             17545..17892
                     /gene="rplS"
                     /locus_tag="U4A29_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO0679827.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            18150..18443
                     /locus_tag="U4A29_000684"
     CDS             18150..18443
                     /locus_tag="U4A29_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEO0679828.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            19286..21613
                     /locus_tag="U4A29_000685"
     CDS             19286..21613
                     /locus_tag="U4A29_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO0679829.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            21739..22068
                     /locus_tag="U4A29_000686"
     CDS             21739..22068
                     /locus_tag="U4A29_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEO0679830.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            22108..23112
                     /locus_tag="U4A29_000687"
     CDS             22108..23112
                     /locus_tag="U4A29_000687"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEO0679831.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            23109..24538
                     /locus_tag="U4A29_000688"
                     /pseudo
     CDS             23109..24538
                     /locus_tag="U4A29_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            24744..25916
                     /locus_tag="U4A29_000689"
     CDS             24744..25916
                     /locus_tag="U4A29_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO0679832.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(26078..26533)
                     /locus_tag="U4A29_000690"
     CDS             complement(26078..26533)
                     /locus_tag="U4A29_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679833.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            27123..27545
                     /locus_tag="U4A29_000691"
     CDS             27123..27545
                     /locus_tag="U4A29_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0679834.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(27711..28904)
                     /locus_tag="U4A29_000692"
     CDS             complement(27711..28904)
                     /locus_tag="U4A29_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO0679835.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            29128..29505
                     /locus_tag="U4A29_000693"
     CDS             29128..29505
                     /locus_tag="U4A29_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO0679836.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            29493..29831
                     /locus_tag="U4A29_000694"
     CDS             29493..29831
                     /locus_tag="U4A29_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO0679837.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            29965..30408
                     /locus_tag="U4A29_000695"
     CDS             29965..30408
                     /locus_tag="U4A29_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEO0679838.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            30670..31485
                     /locus_tag="U4A29_000696"
     CDS             30670..31485
                     /locus_tag="U4A29_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO0679839.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            31495..32157
                     /locus_tag="U4A29_000697"
     CDS             31495..32157
                     /locus_tag="U4A29_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEO0679840.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            32362..34026
                     /gene="alsS"
                     /locus_tag="U4A29_000698"
     CDS             32362..34026
                     /gene="alsS"
                     /locus_tag="U4A29_000698"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO0679841.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            34039..34749
                     /gene="budA"
                     /locus_tag="U4A29_000699"
     CDS             34039..34749
                     /gene="budA"
                     /locus_tag="U4A29_000699"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO0679842.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            34879..35376
                     /locus_tag="U4A29_000700"
     CDS             34879..35376
                     /locus_tag="U4A29_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO0679843.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            35561..35821
                     /locus_tag="U4A29_000701"
     CDS             35561..35821
                     /locus_tag="U4A29_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679844.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(36183..36422)
                     /locus_tag="U4A29_000702"
     CDS             complement(36183..36422)
                     /locus_tag="U4A29_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679845.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT        12338 a         6418 c         7304 g        10643 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt
     3481 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
     3541 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
     3601 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa
     3661 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
     3721 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
     3781 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
     3841 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
     3901 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
     3961 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
     4021 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
     4081 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
     4141 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
     4201 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga
     4261 tttgaactca tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt
     4321 cgttgcttca cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat
     4381 tagtttacgt gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt
     4441 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg
     4501 gcagagaaaa cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat
     4561 ctgagtgcgt gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca
     4621 ataggaagaa gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc
     4681 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
     4741 tctagtatga ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat
     4801 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
     4861 ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg
     4921 aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga
     4981 taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata
     5041 aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc
     5101 gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa
     5161 aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg
     5221 aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag
     5281 tagaatccta taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca
     5341 tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa
     5401 aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag
     5461 atgacattgt tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag
     5521 ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa
     5581 taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag
     5641 acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag
     5701 aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca
     5761 tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga
     5821 tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt
     5881 acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc
     5941 ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata
     6001 aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac
     6061 aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga
     6121 tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc
     6181 aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg
     6241 cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat
     6301 atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt
     6361 acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc
     6421 tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc
     6481 aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc
     6541 aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat
     6601 atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa
     6661 tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     6721 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     6781 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     6841 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     6901 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     6961 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     7021 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     7081 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     7141 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     7201 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     7261 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     7321 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     7381 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     7441 agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt
     7501 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     7561 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     7621 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg
     7681 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt
     7741 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     7801 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     7861 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     7921 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     7981 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     8041 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     8101 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     8161 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     8221 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     8281 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     8341 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     8401 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     8461 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     8521 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     8581 tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc
     8641 atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc
     8701 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     8761 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     8821 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg
     8881 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     8941 gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     9001 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     9061 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     9121 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     9181 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     9241 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     9301 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     9361 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     9421 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     9481 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     9541 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     9601 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     9661 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     9721 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     9781 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     9841 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     9901 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     9961 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
    10021 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
    10081 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
    10141 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
    10201 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
    10261 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
    10321 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
    10381 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
    10441 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
    10501 gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg
    10561 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
    10621 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
    10681 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
    10741 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
    10801 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat
    10861 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
    10921 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
    10981 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
    11041 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
    11101 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
    11161 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
    11221 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
    11281 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
    11341 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
    11401 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
    11461 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
    11521 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
    11581 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
    11641 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
    11701 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
    11761 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
    11821 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
    11881 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactat gacgagaaaa
    11941 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
    12001 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
    12061 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
    12121 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
    12181 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
    12241 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
    12301 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
    12361 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    12421 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    12481 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    12541 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    12601 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    12661 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatattaaaa
    12721 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    12781 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    12841 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    12901 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    12961 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    13021 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    13081 gtatcggcag aaagggcgaa taaagcatgc gaaaatcata ttttatggtg ctataagttt
    13141 agatggctat ttggcaacga aaacggatga tcttcaatgg ctttttgaca ctccaacagg
    13201 taagcaaacc acttatgaag ctttttatga aacaatcgat acgaccatca tgggcagaaa
    13261 aacttatcag gaagcaaaaa aatatttgga gaccgaagca atttatccag agaaaaagaa
    13321 tttcgttttt tccacccacg cacaattaca attgccagat gctgaggtcg taaacgaaga
    13381 tcctgttgct tttctaaaaa atctacaaat gacagaagga aaagatatct gggttgtggg
    13441 tggcggtcga ttgatcaaac cgctattgga acatcaaatg attgatgaat ggtatatcca
    13501 gattacccct gtattattag gggacgggat tcctttattc caaaaaggaa catatgaagc
    13561 gcgttttgaa atgctagaca cgacacgatt cggtgaattt atcgaattgc attatgtacg
    13621 aaagtagaag taaataaaat aaaaaaggca actatatgca gaaatgtcct gtacatagtt
    13681 gcctttttta ttaccgttat gctttttact ctgttaattt gttagaaaaa tctagatacg
    13741 catctaaaat cgtttggaag aaacttaatg ttccttcaac gatattacca ttttcatcaa
    13801 ttagattcgc aacgtttccg atatatgctt ccggttgctg caaggttggc acattcaaaa
    13861 agacaagtga ttggcgtaga tggtggtttg cgccaaagcc gccgatccca cctggtgatg
    13921 cagtaacgac taaacctggt ttatgatccc aaacactgtg tccataaggt cttgagccta
    13981 catctaaagc atttttcaat actgctggca cagaacggtt gtattcagga gtaacgaaaa
    14041 cgacaccgtc tagtcccctc acttcctcac gaaaacgagt ccattcagca ggagcctgtt
    14101 ctggagtttc gagatcctca ttgtaaaatg gcagatcatc gatttttaca aatacacttt
    14161 cgtacccttc aggaagcaat ttcgcaaaag cttctgctac ttttttgttg taagaatctt
    14221 ttcttaaact accaataaaa aatccaattt tttttgtcat acgatttttc ctccttcaaa
    14281 ctatctataa taatcttaca aaagataagt aactggagca aataaaaaca cttactgaca
    14341 aaagctacat agaagataga ttacttattt ttaacaaaaa agaagtctaa aatcttctat
    14401 tttctaaaaa aaatcgaatt taatcaagct gtaaagaaaa aaagctttac agggtaaaaa
    14461 aaaactgtta cactacatct tgtgaataaa acaatggagg tggaaataaa taatggcagt
    14521 taaaatccgt ttaaaacgta tgggttctaa aaagagtccg ttttaccgta tcgtcgtagc
    14581 tgattcacgt tctcctcgtg atggacgttt catcgaaact gtaggtactt acaatccttt
    14641 gaaagaccct gcagaagtag ttttaaaaga agacttagtt cttgactggt tatcaaaagg
    14701 tgcacaacct tctgacactg tacgtaacat cctttcaaaa gaaggcgtta tgcaaaaaca
    14761 ccacgaagct aaattctcaa agaaataagg tgaccggata tgaaagattt aagcgactta
    14821 gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt agacgtcgta
    14881 gagtcgaaag acttttacga gtacaatcta acagtggctc ctgaagatat tggccgtatc
    14941 attggaaaac aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg tgttcggatc
    15001 aatgcaccga aaaaagtgcg tttgaatatc atcgatgata aggaaaagta aataaacgtg
    15061 ctgtactcga attttcgagt gcagcttttt tgtttttttt attaattgaa gaaatgatta
    15121 tttggcaaaa caagcaagaa agcgtatcct tataggagga gggagtgaac agccaatgag
    15181 aaagaaaatg ttatttattc catttgctct attgttaagc gcttgtagtt cggttactca
    15241 agaatcagac aatacacatc tatcaagcag tatatctgaa actgttacca gtccagaaaa
    15301 aagtagtacc aaaacggctt ccaccactga acaaacaatg gctacctcaa ataatgagaa
    15361 aaagactgct ttagaccagt tgaaagaaca acagccaaat gttcccatgc ctctagatgt
    15421 accagtctct tcaggctatc tcaatatcgc agcgacacat acaaagcaag gttattctat
    15481 tttatattac agaacagatc gtcccctcgg gttgaatgct gacgaattga atcaagaaac
    15541 gcctattgca acctatctat atcaatatgg gtttgcttct agtcaagaaa caatccaagt
    15601 tttgcagcca tttgagatcg acacaaatgg tcagcaagtg gacttaggtt ctcgaatcac
    15661 agggtatcag caaggtgcag caggttcaag ttttcttgaa tggcaagaag gaaactggcg
    15721 tattcgaatc cgtgggaata atatagaagg ccaagatccc ttactattag ctaaagaaat
    15781 cgtagcttat ttggaagaaa acagtctacc agcacctgaa caatttggta aaattactgt
    15841 tgatatgggt gatacgacga atcgagccgt agaagtaagc tggcaagaac cgaaaaatgc
    15901 ctatacgatc actcatcaag atcctatgtc tgcgttgaag atggcagttt ctatgaagag
    15961 gttgtgaaat cagcgcgctg atctgagccc atcatttaac tccaagtcac aggaagtaat
    16021 caaaaatagc acgccgcatt tttgcataat tttggcttaa taccttaaaa gaaaactaga
    16081 attgcctctt cacacatgct aaaataaaac agacaaaaaa gaaatgggga tataagttga
    16141 ctgaatactt gaatgtagga aaaatcgtaa acacacaagg tatcaaaggc gaagtccgtg
    16201 ttatctcaac aacggatttc ccagaagaac gttacaaaaa aggaacggtg ctaacactat
    16261 tccaagacgg aaaagcacct gtggaattaa cagtcaaaag tcatcgcaaa cataaaaatt
    16321 ttgacttatt gagttttgaa ggacatcctt ctatcaatga tgtggaaaaa tatcgggatg
    16381 gcatattaaa agtgtcaaaa gacaactctg tagatctagc tgaaaatgaa ttctattacc
    16441 atgagatcat cggtctgcat gtattagaag atggaactga gttaggtaaa gtaaaagaaa
    16501 tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg tccgaaaaaa gctgatatat
    16561 tgcttccata tatcgcttct gtcgtcaaag aagtcgattt agaagctgga gtggtccgtg
    16621 tagaaattcc agaaggactg attgacgatg aagattgatg tattgacttt atttccaaga
    16681 atgtttgaag gtcctatggg ggaatccatc atcggtaagg ctgtagataa aggattatta
    16741 gatatcaata tctctaactt ccgcgattat tcagataaca aacatcagac agtagatgat
    16801 tatccatacg gcggtggagc aggtatgctg ctcaaagtcc agcccatcta tgataacatc
    16861 caagccattg aaaaagctgc accggatgtc aaaaaaagag taattcttct tgacccagct
    16921 ggtaaacgat tcgatcagaa aatggcagaa gaattttcac aagaagaaca cttagtattc
    16981 atttgtggtc attatgaggg atatgatgaa cgtatccgca cattagtgac agatgaagtt
    17041 tccttaggag attatgtcct tactggggga gaattaggtg cgatggtcat gatcgatgca
    17101 acagttcggc ttttgccaga tgtgttaggg aatcagactt cagctcaaac ggattcttat
    17161 tcaaccggtt tattggaaca tccccaatat actcgcccgg cagaatataa agggatgaaa
    17221 gtaccagaag tattgacaaa tggaaatcat aaattgattg aagaatggca attaaaagaa
    17281 tcactaagaa ggacctatca acgtcggcct gatcttttgg aaacacttga atggacacct
    17341 caaatgacga aatttttaga agaaatcaag aaagaagaac aagaaaaaac agcagatgaa
    17401 gccaagtgac tttttctaaa atcttcttta cagtaggttg ataatatgtt agtattttat
    17461 ggtgagtgca aaagcactta actattacga tattccgctg tgagaaaagc tcataagaat
    17521 atttggaata aggagaagaa aaccatgaat ccattaatcg aagaattaac aaaagaacaa
    17581 ttacgttctg acatcccagc gttccgccct ggtgacactg tgcgtgttca cgcaaaagtt
    17641 gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg ttgttatcaa acgccgcggt
    17701 gctggaatca gcgaaactta tacagtacgt aaagtttcta acggagtggg cgttgaacgt
    17761 acattcccat tgcatacacc acgtgttgca aaaatcgaag tagttcgcta cggtaaagta
    17821 cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa aagctgctcg tatcaaagaa
    17881 atccgtcgtt aatcattgtt atcagactga gaaagaaccc ttggtattca agggttcttt
    17941 tttcttagca tgtaatttga acaccctgat ccagtttttt ctttaattat tggtagggga
    18001 gaataatgga ttagaataaa tctacctata agtagtagta aaaaaaagag cactatgata
    18061 atatctttct taagagtaac cgctttaata aaaaatccag aatattctaa cataaatgga
    18121 gtattcttag aatcaaaagg agaaagaaga tggtttatac ttatacggag caagaaaaga
    18181 tgaaagtttt agacacattc atgaatgatg gaaaagtaaa acaagtacca agttcaacaa
    18241 aaaagaaata catcctattg aaagaagtct tgaaacgctt tgaccatgga gtgacttata
    18301 cagaaacaga ggttaattcg atactgctga acgtattttc tagtggcgat tatgtcgaac
    18361 aaagacgtta tctaatcaca tttggctttt tcaaaagaag ttcagacggt cgggcatatc
    18421 agatgatggg aattgaaaat tagaagtttt aatttatact cattgaaaaa agttcaagta
    18481 tgcgatattg gttctacacc gcatacttga actttttttg tcatctgtat ctataataca
    18541 aagccaagaa cgttattcaa aatatggata gctatgataa aagctaatga gcgattcgca
    18601 gttaaataag caatcgtcag taaaatcgtt ccaggagcgt acatgaaaaa atcaattgga
    18661 tgcgttacat gaataaagat aattctcttt tatttgtacg aataaatacc aaaacgtacc
    18721 tcctaaaatt agattttgga cagatgccct ttaaaaagat aacaagattt cgttcttgaa
    18781 acaatatata tgcttttatt ccaatgaggt tgtgaaaaca gtattttgtc ctgagcgtta
    18841 gaaaattgaa aatggggaat agcaactcgt actttaccat tttttaagtg aatacaagat
    18901 cagctaattg aacaccgttt attcgtgatg agggacacaa cactgcagtc gacacatttc
    18961 tcttttatgt tcaaagataa caaatattta aaacaaagaa aaatagtaga taatcccgtc
    19021 ctaactcttt tattcttccc tcatagcttc caaatttaag caatacttca attaatcaag
    19081 ttttatcttt ttttttcaat aatcgtaaaa aaagagtatt ttttatttaa aaataaaatc
    19141 tttgtattaa aggacaagac taaaataacc ttagaataaa aagagaaaaa aagccccaaa
    19201 ctttctatac atcaataaaa taataaaaat taaatttaga ataacgtgta aaggattatt
    19261 tgatgatttt ttattgagag gagggatgaa aatgattaag aaaactcaaa ataaaatcca
    19321 acgtatatgt caaaaacatt ttgtttctag actcaaagct gtatctaaaa gaaaatactt
    19381 acatcccatt atgatttcga tcatcttgat ttttactttc ttagggagct tatcaagtat
    19441 ttatgtaaac gtttatacag aaacgtcaaa ttttgctaac caaacgacag aggagacaaa
    19501 tagtccaccc tattctgaaa tacagtccat agaaagcacc aatcagtcta gcaacaacga
    19561 gacaacctca accacatcct cgactcaact gattgatccg acagaaaaag aaatcacagc
    19621 agcaaaagca gcaggggaga aagcttattc agaaacaggt agaagccaga catttagtac
    19681 tgttgcggcc actgcgaatt tcaacggaca taccatacca gatggatata cagtgatata
    19741 tgccaaagat ggagtcattg ttgcggttaa cggatcgatg agtgcggata aaactgcgtt
    19801 acaaggcgta accaaagcaa tcgcttttat tgataaccgt ccagatatcc ataatctgtt
    19861 tattggctcc acagtaaatg gtactactat cacattccca aaaagtatct atatgggggt
    19921 ggctaccgct ggaaatacga tcgattcagt tacacccaat gcggataaaa gttattcttt
    19981 tacaccctca acatggggag ggcttcctgc cggaggaggt atgagtactt tttcaatcgg
    20041 tatacagtta gcaaatgata ttccattcac ggggtactcg atcaaaaatg ggcagatcgt
    20101 tgatggtaag ggaaatgttt caaattttcc aacctatcaa gcagcaacgg ttcaggtcaa
    20161 tgcgaatcgg acaggaactt tttcttatac agtcaatggt ggagcagcaa aaatcggaac
    20221 gtacgggact acattatctg gaatccatgt gggtgacaaa gtggttatca ctccaacagc
    20281 agtaacgggc tattcttttg cacaaacaaa cggttctttc acagccctag aaagtaacgt
    20341 cgatacggtg acctatacag gcaaacaaac accaatcact attaagtatc aagactattt
    20401 tggacagatg attgctcctt ctaaaataat gaatcttact tacggaagtg ctgcacaaga
    20461 cctgacgact aatacgccta ctatcaacgg atataccttt acagctgttt cagcgacaga
    20521 aacaaagaac caatcagcaa tgagtgtttt agctagttta gatacaaatg gaaatgtaat
    20581 cgtaaaggac gcaaatggaa aacagatttc tgaagtcatc ctatattata agacaaatgc
    20641 aacagtatca atcaatgcaa atggcagtaa gtactatgat ggtctttctg ttgtaccgat
    20701 cgttaaatat gcctttaatg ataaaacaga atcaacatcc cttttaaaca atgatctcag
    20761 tattgatcaa atcacatgga atccaactga tttcaaagcg atgaatgaaa agggagttga
    20821 agtaactgcg cctactgaaa ttggaaccta tacgtcttgg caattgacaa aaagcggact
    20881 agctaaacta gctgccagaa caaactattt atttactatc gtgcagacat ctgatgtcta
    20941 tacgattaag caaatcagcg gtgcggtaga gctcagtggc agtaagacat atgatggaaa
    21001 agcaggtgtc ccgtcaatcc atgtcaagct agcagagggt gtcactagtt cgcttactcc
    21061 agtacaagtg gcacttagca gtatggatta cacagcagat gcacaatcag caaaaaatat
    21121 ggttaatgct ggcagctata caattaaact aacgaatagc ggaatagaca aagtcaaagc
    21181 agcaaatccc aagttgtcat ttactgatat agccaacaca tcaggaactt ataccatcta
    21241 taaagctaac gcagtgatta ctgtcgatga cgctgccttt aattatgatg cccaaagtca
    21301 ctcgatttcg gttggaaatc tccacgttac cggtgttgtc ttaggcgaat cattggatta
    21361 taccctaaca gttaacagcc ggacagatat tggatcacaa atagtaacga ttagtttagg
    21421 cgaaggaagt gtaaacaata attatacgat tatcgcaaca gataccgcta agttgacgat
    21481 caatccgact ctcttgcttc cttcgacagg tgggataggt ctactgccct ttgtcttttt
    21541 aggactgatc tttatttttt ctggcttctt ttattttatc catcgtaaga aaaaagcagg
    21601 tgaacagcga tgaaaaaaac aaaaaatgta cgtgtggata tatttgctgt tctctttttt
    21661 atcctactgg tcttttttat aggttctccg actgtgtcag ctagagaatc acctattaca
    21721 tttactttgc ataatcgaat gttcgaaagc gataagatgc caagtatcag tcaaaacgac
    21781 ggaataaata tcccggataa ctttcaagga gtaaaagggt tgaacggcgt tactttcgct
    21841 atatatgatg tctcggatga attttacaag ctgcggtcag aaggaagtag tgtggaagac
    21901 gcacaaagaa aactagcaca aaaaagtgat agtgaaaaaa tactggcaga aaatgtgaca
    21961 aaaacagtag acgaggaaga gggcattgct tcttttagtg catcggacaa agatgagcaa
    22021 ggtcgtgacg ctgtttaccg atttgcggaa ataaagactt cagattaagt aaaagaaaaa
    22081 tcagcaccat ttgtcgttgt gcttcctgtg acagattcat ctaatcataa attgacgacc
    22141 atccacttgt atccaaagag cgaacaaaaa atacctgctg cactcacatt aacaaaaaca
    22201 gtagagaata agcagacaga ttttgcagat ggggataaag ttccttatct catcaccgta
    22261 acaattcctg agaacatcaa cgagatagga acctatacga tcaaagatac agctgatcct
    22321 cagttatggc tagaaccaga aacgatcgat tttttgattg gtggagacaa gattcataca
    22381 tttcacacac aaaaaacaaa acatggtttt gtcttatcag attctggaaa agatcttagt
    22441 tcgtttgctg ggaaaaaatt aacgatccgt taccaaatga cattgaaaga aaacagtgaa
    22501 aaaacgacat ttgataacga tgtacgtttg acgactgaca atcaaacagt agaaacacat
    22561 cttgccatcc aaacaggagg caaacaattt gtcaaagtgg atcggcaaac gaaacaaaaa
    22621 ctagcagatg ctcaatttct agtcaaaaaa aatgaccaat acttagcgca agaaaacggg
    22681 ataaatcatt gggtagccaa agatgattca agagcaacga tttttacttc cagtaaagat
    22741 ggaacatttt ctgtccatgg attgtctttt ggaagttacg aactagtgga gattaaaccg
    22801 cctaaaggat atatattgag tcaatcaacc attctctttg aagttgaaaa aggcagttat
    22861 gatccgcttc atcctatacc attggaaatc atcaacgaac caaaagcaaa tccgacgaat
    22921 aatggaaaaa cgccagttca gatgcagaca aatggtccat caaatgaaaa aaatggagga
    22981 agctttccaa aaacaagcga agagatgctt ggaggtttct caattttagg gttgttgcta
    23041 gtcctttcaa cagggacagc ttggttttat aaaaaacaac aaaaaggaaa caataggagg
    23101 gaaataaaat gaaaaacatc aaaacaaaaa tatggacgtt tttaggaact gctatcatgc
    23161 tgcttccttt cgttctcggc ctaggaactg cagaggtttc taggccgtat cacctacacc
    23221 agaaaatgtt acggttaatt tgcataaatt gaaattcact tcagcaccag aaaatcaaat
    23281 aaacaacgga acggaattga catttccgaa tagcgaaccg ttgaacggcg ttgaattcaa
    23341 cgtatatgac atcactgcta cgtactatcc aagtaaagac acagcagttc cagcagacgc
    23401 tacacccttc gcaagtgtaa cgacttctgg tgaaggatta gctaacctga ctcttccagg
    23461 taaatctgat gggaaagatg ctgtatacgt ctttgtagaa acaccaaagc ccggtgtcga
    23521 aacgtcgcca aatatcgtgt taagcttacc cttttataac ccgaattcat cagaccctga
    23581 caaaccttta gacacgattc acctatatgc taaaaatgtc atcaaagaaa gcaatatcaa
    23641 aatccagaaa gctgggaact tttcaggggt agataagtta gcaggggcgc aattccagat
    23701 caaaaattca gagaatcaat tcgtgaccgg ttttgatgaa aacactggtg tcgctctcta
    23761 tgatcctgct gagtcagcgt tgacattcac aactgatact gaaggatcca tcagtgtcaa
    23821 tggattaata gacggcaatt atactttagt tgaaacaaaa gcaccagatg gttatcaatt
    23881 aaccaatcca caaaaagaaa ctacttttat agtaaaaaac ggaaaattgc aacaaacaac
    23941 cggtatcatc catggcgcag atgaagccaa tcttgggaat ccaaacggtg tagtcaatcg
    24001 ggaaaataca actacggata acacgattac tgtagaaaat gtccaaaact acggtgattt
    24061 ccacttcatc aaacaagatg tgaacacgca agaaaaatta gccgatgcag aattttctat
    24121 tctaagagca aaagatccaa ctgttaaatt aatgaaaaaa atagcaaatc ttgcctcagg
    24181 agaataccca tatgcttggt cagatgaagt cacaggttct gactgggaag aagtgaaatt
    24241 gatttctgat tcagaaggga aattttcaat cgaagggctg aaacaagggg catactgcct
    24301 tcaagaaaca aaagcaccag aaggctacgc attacctaag aatgaagctg cgtacactga
    24361 atttacagta gatgatgatc caacaacttc tgatgaaaca acgatcgata accaacctaa
    24421 aggaatcttg ccttcaactg gtggtatggg gatcatcgta ttcatcttgg taggtactgc
    24481 tttagttgga ggagcagtga tctacttcaa aaaacgccat caagaaacag aagcttaaac
    24541 tattggaaac aaaaatcata cgataaataa acgatagtga ttcaaatggg gagaaaggtt
    24601 gtgacaagtg gtcggtctct tggtattcgc tcaacaaacg gcaaaatagg aggaactatt
    24661 ttgccgtttg ttgagcgaat actcaggtca aggcgctgat ttcacaacct cttcatatac
    24721 atattagaaa ataggtggac atgatgaata aaagcagaat aagaatactg gacatcttca
    24781 tggcaatcat tttagtcgtt gggatcggga tcttttctta tccttttgta gaagattcac
    24841 tgaatgattt tcttgcccag caaatgatca tccattatca aaagcaagca tcgaataaga
    24901 attcagagga aataaaaaaa cagcaggaaa aaatgacaaa gaaaaaccaa cagttggcag
    24961 agcaaaacgt atcaccaggg ataggcagtt tcaaccaagc agttgatgca aaagcattaa
    25021 aagatttacc tagtaatgcc ttttttatgg aacatatgct tggagtcatc gaaatcccaa
    25081 aaatcaatgt cagcttacct atattcgatc agacaacgga aatatttttg caaaaaggaa
    25141 cgtcattatt ggaagaaagt tcttatccga ctggcgggaa aaacacccat gccgttcttt
    25201 ctgggcatcg tggactccca gaagcaaaat tatttacaga tttgcctaag ttgaaaaaag
    25261 gagaccagtt tttcatccag atcaatggaa agacattggc gtatcaagta gaaaaaatcc
    25321 aagtggtctt acctgatgaa gtagattctt tgggtatcca aaaaggcaga gatctcgtga
    25381 ctttgcttac ctgtactcct tatatggtca atacacatcg actgcttgtg acggggcatc
    25441 gtattcctta tcaagcaaaa gaggcaaaaa aagcgatcca aggaattgat caatggaaaa
    25501 aatggaaatt ttttatttgg tttatcggta tactacttgg atcaatcggc ttagtttggc
    25561 tactcatcgc ttatttagat tctttggcca tcgctaagcg aaactatcct ttatcttttt
    25621 atgtaaagaa cacaaacgga cgaccaatcg aaggaatggt cttttctgta aaaacgctca
    25681 acggcaaaca ctatattact agagaaaaag ttccatttgt gaaagcttct gatgaatacg
    25741 gactagtgag attttctgat ttaaaaggca ggaactatcg cttgcaacat gaggagctcc
    25801 tgcttaaaat ccatgtaaaa cataaacatt cgaaacagtt ttcaatgaaa ttgaaaaaag
    25861 gaagatataa actgcgaaaa gaaaaagagg tgtactattt gattgagaaa gaatgaatga
    25921 agatgtccaa aaaagcagag ggaacagact aaatgaaaaa agagagaagc tgacgaaatg
    25981 gacgtgtaaa ctgctatttt tggtttacta tctggaacgc cagcttctat ttttttatgt
    26041 ttcagaagaa ttttattatt atagtataac ttttgaatta gtttccataa catttggagc
    26101 aaggggttaa accacgagct tgagcttcgc ttaaagtggc aggggtatac gtaccatttc
    26161 cacatttatg tgtatggtat tttgaaccgg tcggtgtgac atagacagtt tgttcattat
    26221 tttgctcagg agctgcggct tgtgcctgct gagaggcctg ttcagctgct gcttgctgtc
    26281 gcgcctgttc tgcttgttgc tgtgcgattg cttcttgttg cgcttgttct gcttgttgtc
    26341 gtgcaatttc ttcttgcttt ttccgttctt cttctgcttc ttttgctttg attgtggaat
    26401 caattgcagc aagacggctt gtcaactctt gtttgttttc aggtagcgct tggatagctg
    26461 ctgaagcagt tgcatagttt tcttttgtag gatttgcttc agcttgtgac aaagcagatt
    26521 ccgcttctgt cacttttttt tctagtgcag ctttttccgc tttttgtttc tcttcttctt
    26581 ttttcttttc aatttgttct tgttctttcg atgaatctgc cgtactttcg gagatcgtgg
    26641 tactgctgct ggaagcctga gctgaacttg tgctttgagt agtagacgta gtagcaccac
    26701 acccactcaa caaacttcct gcgaccaaaa acaatgcaac taattttttc actaaatttc
    26761 ctccttaagt aaaaaacaac cgttttcaat aaatgtcctt ttcaattgtt tacttacttg
    26821 ttataataat tgtgttatga atagtgattg aggaaactta atctaatccc gttttgatac
    26881 acgggatttt ttgctatagt tctgttatta agtatgcaat aataaaatat gtacgttatt
    26941 ttattattag attattgaca tttatataat aaacgattaa atgattcata tcaatagaat
    27001 acttaggaaa aaaagagaaa taataacagg tttatgcact attgacggtg tgacaatatg
    27061 atatttgtat ccgttttttt tcttgtatgt agtatagtaa agtagataca ggaggagaaa
    27121 atatgcagac ttttttaaca aaagtttctc aaaagaaaat taaaatgatt catttactgc
    27181 ttgaaacaga acgttggtgt tcaatagaag aactacaaaa cgaattgaag gtatcaagta
    27241 agtctatctt gaattatcta acagatttag aagaattgtt ccaaaaatat cctgataagg
    27301 tcattttgaa gaatgagaat aatcggcgct tttctatgga aaagcaggag aacttcccaa
    27361 tctacattat ttatcttcat ttctacagaa aatcatataa ttatcatttg attgaattca
    27421 tgtatcaaca tcctgagaaa aatttggaag attatgcaga cgctcaatat acgagtgtat
    27481 ccacagtttt tcgttatgct aagttattag taaagtactt tgaaatgacg gacgtcaaag
    27541 aatagtagcc gccagataga aggaaattag cagttgaaaa agctgccctg catcaagtaa
    27601 tacgaccagc caaacggaaa cagtgtctca tgtttttgat ttggctggtc ttattaccgc
    27661 agatgcaagc ttttgccaca cctctttctt gtcatttaca gactcaacta tcaatgctgc
    27721 aagctttccc ttaatgctgt aacaccagta agagttgtca agaccagcag actgactagg
    27781 taaataccgc tcaaaaacaa gattacggca gttaaactat aatgatccat caagaaaccg
    27841 atgacgatag aagaaaaacc accaacagct cgtccaaggt tcataatgaa attattcgcc
    27901 gtcgcgcgga tttctgttgg atacaaacta ctaatcactg cgccatatcc accatacatg
    27961 ccattgataa agtagccgac tatcacagcg gctgctacca aactccactg gctatgggct
    28021 aacacaagta gatagacaga taaggatgaa caaattaaaa acaaagaaaa ggctagttta
    28081 ggccccaatt tatccatgat tatgccaaaa gtcatcatcc ccaaagacat gcctaaaata
    28141 gtactgacca tccacaatga agacccagaa accgaaagcc ctaatcgatt ttgcataatt
    28201 gaaggcagcc aattcattaa cccaaaatag ccggcaattt ggacagttac catgatacta
    28261 aggccacatg tttgccaagc aagatgtttt gattcaaaca gtctgctaag tttggttttc
    28321 ttttcggcag attgtacaga tttgaattcc tttggttcag tcaagccgcg acgaatgaac
    28381 aagaccatca acaccggcag tacacctacg acatacaaca tcttccaacc aaacgttgga
    28441 atgatcacag cagctaaaat cgcagccaaa gccgcaccga cctgcgcacc aataccagca
    28501 accgaagaag ctcgtccaat ttgttttttt gaaaaacttt cggaaatcaa ggacatacac
    28561 gccccgtatt ctcctcccgc tcctacacct gcgataaagc gaaagagata aaccaagtag
    28621 atgtttgaag caaaatacat caataaagaa gcaatcgaaa acagaaggac cgtttgagaa
    28681 aatactttca cacgtccata tttatcagct atgatgccaa aaaaaatacc tccaatgagc
    28741 atccccaaat tagtgatcgt tgaaatgaaa cccgcttgcg taccactaat atgaaaagaa
    28801 gtaatcattg aagataatga aaaagctaaa aacataatat ccatattctc taaagcaatt
    28861 cccgccgatg tcgataataa taactttcgc tgatacgttg tcatttttat accccttttc
    28921 ctaaaatttc cctaaattgt taaaagtata tccaaaaaag aataaaaaaa caagactttt
    28981 ttatttgaaa taatacacaa acaaccgata tagataatcc agcttagttt taatagaaat
    29041 gagtattgac ttggagtaca cttaaagttg tagaataaat aaaatacata agtaaagtgg
    29101 caagaaatta agagaaagag tgatcagatg aatatcaaac aagtcagtga agaaaagggc
    29161 atttcggccg atacattgcg ctattatgaa cgaatcgggt tgattccacc agttaatcgg
    29221 acaaaaggcg gaatccgtga ttatacagaa gaagacctaa aatgggtcga ctttacgatc
    29281 tgtatgcgaa atgccggatt atcgatcgag tctttgattg aatatattcg cctatctagc
    29341 gaaggagaag caactgtatt tgagcggcgt aagcttttga ttgaagaaag tgaacttttg
    29401 acgaaaaaaa ttgctgaaat gcaagaatgt ttggaacgtt tacaaggaaa aatcaagaaa
    29461 tatgatcgtg tgttatctag aaataaaatg gaatgtatca agtaaaaaaa tcaaggagca
    29521 tcgctatgga gttacgctta ctaaaatatt tttggacaat tgcagaagaa ggaacaatct
    29581 ctcaagccgc agaagttcta catcttacac aaccaacctt aagccgacaa ctgaaggagt
    29641 tagaagagga actaggaaca gaactgtttg tacgcgaaaa ccgcaaaatg atcttgacag
    29701 aagcaggtta ttttctaaaa aagccgagca gaagaaattt tagatttaac gaatcaaacc
    29761 actcaagaat tcgagaacag aaaaaaacaa ttattcagcg gccgatttac gatcggctgt
    29821 gtggaagctg ataattcgaa tacattggca ctattactag aaaagttgat ccgtgattat
    29881 cccagcgttc aatttcatat ccacagcgga acaagtgatg atatcacgga gtgactagaa
    29941 aaaagcctgt tagatttagc gattttgcta gatccagtag atacagagaa atacgaaacg
    30001 attttgttac caagaaagga aaaatgggga ttgctcattt tagaaaattc tgttttagca
    30061 aaaaaagacg tgataacaaa agaagacttt gcgaatgtac ctcttttgat ctcaaagcgt
    30121 ccagaaatac agcgaatgtt tgctgactgg ttaggcataa aaaaggagca gttgctgatc
    30181 acaggaacgt acaatttgat tttcaatgta tttgcgctaa ttgagaatca ggtaggtgct
    30241 gctttagtca tagaaggtgc tgtaagtcat ctttcaaagc gagtgacatt cgttccgctt
    30301 gacccaatga ttgaaacgaa ctgcatgcta gtctggaaaa agaatcggat tttatcacct
    30361 gtagtcaatg aattgattca ccgtttcaaa gaagcttttg aacagtaata agtatctcta
    30421 atcgaagaaa tttcaataca aaagcgagac gtatgacagt cttgcttttg tattttttca
    30481 tttttaaatc aaaaaaaata aagagattta cataacttgt taaataagcg cttacaaaca
    30541 taagaagaac ggaaatgaat cagctataat aaccgttgtt ctggattgta atcattacaa
    30601 acaaatgaag attataactt tggaggtgtt cgaaatgaaa gaaaaaaagc aaaaattcgt
    30661 tcaaaaaaca tgccctttat tcaatggttg ggtatgattc tgctgattgt attagcttta
    30721 ttgacgatca ttcctttttt atggatggtt tctgcttcat tcaaagcaaa taatgaagtg
    30781 ttcacgattc cgattcaatg gatccctaaa acttggcatt tagagaatta tctggttatt
    30841 tggtctcgta ttcctttagt gacatttttt aagaatacag cttttttaag cgttgtgatc
    30901 acgttgattc agttgtttac ttctagtttt gctgcttacg ggttttctaa actaaatttt
    30961 aaagggcgcg atattttatt tttagcatat atcggcacga ttgctgtccc ttggcagtct
    31021 tatatgatcc cgcagttcat tatgatgaga aaagtgggtt taacatatac gttgtggtcg
    31081 ttggtgctat tacaagcgtt caatgctttt ggtgtgttct tgttgaagca atactacagt
    31141 agtatcccag attctttatg tgagagtgcg cggatcgatg gcttatcaga gtggggaatc
    31201 tatcgcaaga tcattttacc tcttacaaaa ccggcgattg ccagtttgac gatcatcaca
    31261 ttcgttaata catggaacga ttatatgggg ccattcattt atttgtcttc tacggaaaat
    31321 aaaacaatcc agttagggtt gaaaatgttt attgggctat ttgatgcaga gtacgcatta
    31381 atcatggctg cctcagttgt ttctgttctt cctgttgctt ttatattttt agcgatgcaa
    31441 aaatattttg tagaagggat cgcttcatca ggaatgaagg aataggaggt gattatgaaa
    31501 gagcagcgtt ttgacaaaaa tgtttatatg gaattctttt attggttgta tcaatcattg
    31561 gcaagtagcc tttgtttttg gtgtgtcaat atcttgtata tagcagctgc gttccttttg
    31621 tcgatagatg gaaggaatgt cttttccttt attttttctt tactttttat tggtccaggt
    31681 tgcatcgtga tgataggtat gacagaagaa ttagcaaaag aaaaaagttt ttcaccagta
    31741 cgtttgttca taaagacgtt tcgccaattt tggctaaagg gattttttta ttggctattt
    31801 gcttggatag ttagtgtgat aatgattttt gattgtttct tttttatacg tttttcttat
    31861 ggaaaatggt tgattccgtt gtttgtttta cttgcttgtt tatcagtttc ctttagcatc
    31921 aactactggt attttcaggt gagaaatcca gcgtcgaaac caaatcaagt tctacggata
    31981 gcgttctact atacgttgaa aaaatggtat gtgagtttac tagatttttt actattgaca
    32041 agtttgtttc tcttcttttt cgtaaagccc caatggtgta ttctactagg tccttctatc
    32101 gtatttggtt tgatttactt caacaatcgt aaattgatga gaactatgga tctgtagact
    32161 ttataaattg aaactttcaa ttaagaatga cagtttttat gtaaaaactg tcattctttt
    32221 tcattttttg aaacatatgg ctaaacaaat gaaataaaac gtgctatact tttgaatgaa
    32281 acaggtttag aaactgtatc aattatttgt gattatttct aactaataat taaaaataaa
    32341 gcataaaaag gacgatgttc aatggacgaa aaaatgaaac aaggttcgaa atctgtagta
    32401 gaaagtttga ttaatcatca tgttgattat gtattcggta taccaggtgc aaaaatcgat
    32461 ggtgtattta atgaattaga ggatcaaggt cctgaattaa tcgtgactcg tcacgaacaa
    32521 aatgctgcat ttatggcaca agcaatcgga cgtattacag gagagccagg agtagttatc
    32581 gcaacaagtg gtcctggtgc tagtaactta gctacaggtt tggtaacagc gacagcagaa
    32641 ggtgacccag ttttagcaat tgccggacaa gtaaaacgaa gcgatctatt gaagttgacc
    32701 catcaaagca tggataatgc agcactgttc cagccaatca caaaatacag tgcagaaatc
    32761 caagatccag aaacaatttc agaagtgatt gccaatgctt atcgtatggc taagagttca
    32821 aaaaaaggcg cgagctttat cagtatcccg caagatgtag tggatgcacc tgtccaaggg
    32881 aatgtcatca agccattgag cgatcctaaa ttaggttctg cttctgcaga tgatatccgt
    32941 tacctagctg aacgcattag agaagcaaaa ctaccggtac tattagtagg tatgcgagga
    33001 tcaagtgaaa aagaaacgtt agccatcagg caattagttg gaaagacagc attgcctgtt
    33061 gtagagacat tccaagcagc tggagtgatc tcacgtgaat tagaagcgca tttctttggt
    33121 cgtgtgggtt tatttagaaa ccaaccagga gatatgcttt tgaaacgaag tgatctagtt
    33181 attgctattg gctatgatcc aatcgaatat gaagcaagaa actggaatgc ggaaaaagat
    33241 gcgcggatca ttgtgatcga tgaagcgcca gctgaaatcg atccttttat gcagcctgaa
    33301 agagaattga tcggtgatat ctcagccact cttgatttat tgacaggttc attagagcct
    33361 caacaagtgt cagaagatgc aaaagaatat ttagcatctc ttcaagcaga attaacggag
    33421 cgggacattg ttcaatcaaa aggagaagca ggtatcttgc atcctttaga agttattaac
    33481 acgcttcagt caaaagtaac agatgatatg actgttaccg tagatgtagg tagtcattat
    33541 atttggatgg cacgccactt tagaagttat gaacctcgtc atttgttatt cagtaatggt
    33601 atgcaaacgc ttggtgttgc attgccatgg gcgatttctg ctgcattggt gcgtccaaat
    33661 acacaaatcg tatcagtttc cggcgatggg ggatttttat tctctgccca ggatttagaa
    33721 acggcggtac gtaaaaaatt gaatatcgtt catttgattt ggaatgatgg acactacaat
    33781 atggtagagt tccaagaaaa aatgaaatat cagcgggcgt caggtgtaga ctttggtcca
    33841 gttgattttg tgaaatatgc agaagctttt ggcgctaaag gaatccgagc tacaagtgta
    33901 gaggaattag aaaaagcttt agaagaagga tttgcgacag aaggacccgt aattattgat
    33961 attccgattg attaccgtga taatgagaaa ttgggagaaa caatcttacc agatcaattc
    34021 tattaaggaa gtgtcgacat gactgaaaaa atcttatacc agcacgggac attaggagct
    34081 ttgatggctg gcttgatgga tggaacagaa acgattgctc atatattaga aaaaggaact
    34141 ctgggattgg gaacattgca tgggcttgat ggtgaagtca tctttttaga tggcgtagct
    34201 tatcaaggaa gatcagatgg ctcagttgcc caattagatg gaagtgaact taccccctat
    34261 gcagcaatta cagattttac gccagatact agtttttctg tctcaaaaac tgcagatgga
    34321 gaacaattga aaaaagacat attagtaaga gaagccgggg aaaacctatt tttggcagtg
    34381 aagataactg gattattcaa aaatatgcac atccgtatca tgccgaagca agaaaaacct
    34441 tacagaagac tagcaaaaat ttcagagtct caaccagaat tccaacaaag caatgtccaa
    34501 ggaacattag tcgggttctt tacgcctgaa ttattccaag gagttgctgc agcgggattt
    34561 cacttgcatt ttatcgatga tacacataca tttggcggac atgtaatgga ttttgaagtg
    34621 gctgaaggaa aagttgagat tagccgcatc tcgtcccttg tacagcattt tccagttgat
    34681 gatccgactt ttgttcacga aacgattgat tatgctgatt tagcagcaga aatccaacaa
    34741 gcagaataga aaaagacagc gagatgcgtt caaggtgatt aacctgacgt atctcgtttt
    34801 tttgaggagg aaacctagcg tttacaaaaa aactgagcta taatcttctc catagatcaa
    34861 aaaggagcgg tttttatcat gtggacagaa aaacagaaaa aagatgcata cgaaatttta
    34921 ttattacagc aacaaggatt attagaagca gaagataact ggttagcaaa tttagcaaat
    34981 tcatctgcat tgttgaatga gactttgcct gagactgtct ttgcaggata ttatttattc
    35041 gatggtaatg aattgatttt aggaccgttc caagggcgag tttcttgcac acgtatcaag
    35101 atgggaaaag gagtttgtgg agaatcagct gaaaaacaag aaacattgat cgttgatgat
    35161 gtcaaaaccc atgcaaatta catttcctgc gattcagccg cacaatcaga aatcgtagtg
    35221 ccaatggtaa aagaaggtaa tttaatcggt gttttagata tcgattgcgg agtaacgaaa
    35281 gggtatgatg aaatcgatca agcatttcta gaaaaatatg tagatctttt attagcaaat
    35341 tttaatataa atgctttttc atttattaat aactaaaatc gtctattgaa acaaaaaaga
    35401 agagaaatca agaataaaaa gagccggaaa gacagtgggc atttctgttt ttcggttttt
    35461 ttattttttt ataaaatcac aagtgttatt gattttcata atcatatttt aatggtatta
    35521 tcattttaaa taatggaaaa acattaagga tggatgaaat atgaataacg aaactatcat
    35581 tttaggaaaa aaatatattt gccaacctat tggcttgaaa caaaaagtgg ttggagaagt
    35641 aatcagtaaa ttagaaaatt gtgttgtgat ctgcgtagac gaatatcatg acgtcgatcg
    35701 caatgaaata ttagaaaaat gcggaaaagt agtggcaaaa tatactcacg tttatgagtt
    35761 ggaaacaaca gaatatctat ataactatag caacaaatat gaacatactt tagtttcata
    35821 aagatttaaa taaagtgcct tcaaaagcga gaagaccaag cttttgaagg cactttattt
    35881 agttggcagt gattgttcat gatttttgat ttattcaaaa ggttcgccta gagaatctgt
    35941 ctctgtaacg aggcctggtg gagaaactcg ataaaaacct atactgccag aactgtccta
    36001 ttttcggata gacaaactag tagttttaaa aagatcatcc gatccaatca tttcaaaaaa
    36061 tgcaaaactg atatcaggat catttagctc aggcttgtta tcgtaaagca tttgtaaagg
    36121 ttgcatttag ttaggagtaa acgcgaaact atcatcatta tttgctgaag aagatgaaga
    36181 agctaattca gagtttgttt gaaataaagg agacgtgctg acagtttctt taccctgttt
    36241 tttgcagagg tagaaacttt tatatcggaa gtttctaccg gagaattttt ctcatttgta
    36301 gaaattgttg aatcgatact attcgaaatt tgcgtatctg aaggtatgtt ctttgatgca
    36361 gaagtcccac aagcagtaag taacaataaa tttgataaac tgataaacag aattcttttc
    36421 attatgtaca cactccttat agtatcgttt agcatacacc tatgctcagt attaggcaaa
    36481 atcaatgcaa aactatcaag tcttttatag tatctattag tcttagtaat acatatatgg
    36541 agagggtgat atcccagttc aaaccagata cattggttac catctgtatt aacgattatg
    36601 cctctaaacg ctttttttat gaaaaaattt ataagtgaaa acgtaggtat gatattatct
    36661 agaggtaatt tttttgggta tttaaatagt tgctaggagg ata
//
LOCUS       DASOUD010000014        36565 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.014, whole genome shotgun sequence.
ACCESSION   DASOUD010000014 DASOUD010000000
VERSION     DASOUD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.014"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="U4A29_000703"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="U4A29_000703"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO0679846.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="U4A29_000704"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="U4A29_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO0679847.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="U4A29_000705"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="U4A29_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO0679848.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="U4A29_000706"
     CDS             complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="U4A29_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO0679849.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="U4A29_000707"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="U4A29_000707"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO0679850.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="U4A29_000708"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="U4A29_000708"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO0679851.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="U4A29_000709"
     CDS             complement(8052..8522)
                     /locus_tag="U4A29_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEO0679852.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="U4A29_000710"
     CDS             complement(8568..8993)
                     /locus_tag="U4A29_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO0679853.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="U4A29_000711"
     CDS             complement(9060..9329)
                     /locus_tag="U4A29_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO0679854.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="U4A29_000712"
     CDS             complement(9340..10935)
                     /locus_tag="U4A29_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO0679855.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="U4A29_000713"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="U4A29_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO0679856.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="U4A29_000714"
     CDS             complement(14488..15048)
                     /locus_tag="U4A29_000714"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO0679857.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="U4A29_000715"
     CDS             15401..16375
                     /locus_tag="U4A29_000715"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO0679858.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="U4A29_000716"
     CDS             16545..17945
                     /locus_tag="U4A29_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEO0679859.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="U4A29_000717"
     CDS             complement(18023..18745)
                     /locus_tag="U4A29_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0679860.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="U4A29_000718"
     CDS             complement(18824..19330)
                     /locus_tag="U4A29_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO0679861.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="U4A29_000719"
     CDS             19453..21252
                     /locus_tag="U4A29_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEO0679862.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="U4A29_000720"
     CDS             complement(21320..22894)
                     /locus_tag="U4A29_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEO0679863.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="U4A29_000721"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="U4A29_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEO0679864.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="U4A29_000722"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="U4A29_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEO0679865.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="U4A29_000723"
     CDS             complement(24671..25309)
                     /locus_tag="U4A29_000723"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO0679866.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="U4A29_000724"
     CDS             complement(25310..25960)
                     /locus_tag="U4A29_000724"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO0679867.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="U4A29_000725"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="U4A29_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO0679868.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="U4A29_000726"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="U4A29_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO0679869.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="U4A29_000727"
     CDS             complement(29103..29843)
                     /locus_tag="U4A29_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO0679870.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="U4A29_000728"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="U4A29_000728"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO0679871.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="U4A29_000729"
     CDS             complement(31214..32152)
                     /locus_tag="U4A29_000729"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO0679872.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="U4A29_000730"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="U4A29_000730"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO0679873.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="U4A29_000731"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="U4A29_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO0679874.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="U4A29_000732"
     CDS             complement(35312..35617)
                     /locus_tag="U4A29_000732"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO0679875.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="U4A29_000733"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="U4A29_000733"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO0679876.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9885 a         7988 c         6705 g        11987 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcgc ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 caaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc cagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcaattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 gtcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct tcttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagct aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgttcttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg atatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg cttaa
//
LOCUS       DASOUD010000015        35760 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.015, whole genome shotgun sequence.
ACCESSION   DASOUD010000015 DASOUD010000000
VERSION     DASOUD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.015"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..252)
                     /locus_tag="U4A29_000734"
     CDS             complement(<1..252)
                     /locus_tag="U4A29_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO0679877.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEIS"
     gene            complement(407..733)
                     /locus_tag="U4A29_000735"
     CDS             complement(407..733)
                     /locus_tag="U4A29_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679878.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(741..944)
                     /locus_tag="U4A29_000736"
     CDS             complement(741..944)
                     /locus_tag="U4A29_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0679879.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            1019..1669
                     /locus_tag="U4A29_000737"
     CDS             1019..1669
                     /locus_tag="U4A29_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0679880.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1724..2890
                     /locus_tag="U4A29_000738"
     CDS             1724..2890
                     /locus_tag="U4A29_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO0679881.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNSILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(2979..3371)
                     /gene="rpsI"
                     /locus_tag="U4A29_000739"
     CDS             complement(2979..3371)
                     /gene="rpsI"
                     /locus_tag="U4A29_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO0679882.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3387..3830)
                     /gene="rplM"
                     /locus_tag="U4A29_000740"
     CDS             complement(3387..3830)
                     /gene="rplM"
                     /locus_tag="U4A29_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO0679883.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4349..4816)
                     /locus_tag="U4A29_000741"
     CDS             complement(4349..4816)
                     /locus_tag="U4A29_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEO0679884.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4936..6474)
                     /locus_tag="U4A29_000742"
     CDS             complement(4936..6474)
                     /locus_tag="U4A29_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEO0679885.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6554..7240
                     /locus_tag="U4A29_000743"
     CDS             6554..7240
                     /locus_tag="U4A29_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO0679886.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7441..7968)
                     /gene="msrA"
                     /locus_tag="U4A29_000744"
     CDS             complement(7441..7968)
                     /gene="msrA"
                     /locus_tag="U4A29_000744"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO0679887.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(8052..8729)
                     /gene="pgmB"
                     /locus_tag="U4A29_000745"
     CDS             complement(8052..8729)
                     /gene="pgmB"
                     /locus_tag="U4A29_000745"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEO0679888.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8726..9856)
                     /locus_tag="U4A29_000746"
     CDS             complement(8726..9856)
                     /locus_tag="U4A29_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO0679889.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9856..12039)
                     /locus_tag="U4A29_000747"
     CDS             complement(9856..12039)
                     /locus_tag="U4A29_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEO0679890.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(12036..13061)
                     /locus_tag="U4A29_000748"
     CDS             complement(12036..13061)
                     /locus_tag="U4A29_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO0679891.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(13106..13933)
                     /locus_tag="U4A29_000749"
     CDS             complement(13106..13933)
                     /locus_tag="U4A29_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEO0679892.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13930..14997)
                     /locus_tag="U4A29_000750"
     CDS             complement(13930..14997)
                     /locus_tag="U4A29_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEO0679893.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(15023..15859)
                     /locus_tag="U4A29_000751"
     CDS             complement(15023..15859)
                     /locus_tag="U4A29_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO0679894.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15856..16725)
                     /locus_tag="U4A29_000752"
     CDS             complement(15856..16725)
                     /locus_tag="U4A29_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0679895.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16795..18078)
                     /locus_tag="U4A29_000753"
     CDS             complement(16795..18078)
                     /locus_tag="U4A29_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEO0679896.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(18092..19777)
                     /locus_tag="U4A29_000754"
     CDS             complement(18092..19777)
                     /locus_tag="U4A29_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEO0679897.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            20052..21077
                     /locus_tag="U4A29_000755"
     CDS             20052..21077
                     /locus_tag="U4A29_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0679898.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21672..25346)
                     /gene="rpoC"
                     /locus_tag="U4A29_000756"
     CDS             complement(21672..25346)
                     /gene="rpoC"
                     /locus_tag="U4A29_000756"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO0679899.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(25404..29021)
                     /gene="rpoB"
                     /locus_tag="U4A29_000757"
     CDS             complement(25404..29021)
                     /gene="rpoB"
                     /locus_tag="U4A29_000757"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO0679900.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29749..30717
                     /locus_tag="U4A29_000758"
     CDS             29749..30717
                     /locus_tag="U4A29_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO0679901.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30735..31640
                     /locus_tag="U4A29_000759"
     CDS             30735..31640
                     /locus_tag="U4A29_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0679902.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31637..32443
                     /locus_tag="U4A29_000760"
     CDS             31637..32443
                     /locus_tag="U4A29_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0679903.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32579..33568
                     /locus_tag="U4A29_000761"
     CDS             32579..33568
                     /locus_tag="U4A29_000761"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEO0679904.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLKVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33885..34409)
                     /locus_tag="U4A29_000762"
     CDS             complement(33885..34409)
                     /locus_tag="U4A29_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEO0679905.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34696..>35760
                     /gene="scm"
                     /locus_tag="U4A29_000763"
     CDS             34696..>35760
                     /gene="scm"
                     /locus_tag="U4A29_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEO0679906.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        10237 a         7566 c         6600 g        11357 t
ORIGIN      
        1 cgaaatttct acgagatttt tgatttcaaa tatcaaaaaa attttttgat tctcatcata
       61 caagttattt ttcaatgaaa gtctatttct atcttttagt aacatataaa ctaacttaga
      121 acttagtttc attttcttat atttttcatt tacgtttaaa aaaataggat attgatagta
      181 taattctgca tatatatcag aattagaata gccttgcttt tcaactaagc tttcaacttc
      241 tttttcattc attgtcacac ctgcttctga ttttatttgc tataatagat tcaaataaag
      301 ttttctcaga actttattat tgagtgttcc cccgatctgc aaactgacgg gaacactctt
      361 tttaattttt tctacttcat cattattcaa aaaaacacct cgttttttaa atttgatgac
      421 tcaatatcca ttttttgcat tcttcttttg aaaacaactc cattttttct aataaaataa
      481 ctggcaatcc cattctttta aatttcaaaa aagttgtata tgatacattc aaatatttag
      541 ccatctcttt tccttttaga aatggtcttt ctaacccggt atcaattctt gcttgatcaa
      601 tagcctcttt tgtcatttca aaaacataat ttttcaattg attcgtttgt tgtttatcta
      661 acattactgt aattcctgca tccattattt ttctcctctc attttctatt tcttcaatca
      721 gccaactcgc caagttgtat ttatttagat ttcaaatcat taagtaacca atttaccaac
      781 ttttgataag tagatgctct aatactttct ttgctcccat tttcgatttt gacatatgtt
      841 ttccgattta tacctatcaa ttttgctgca tcgtttttag taagtataag atcggctcta
      901 tgtcttctta agcttctaat gaattgatca ttaagagtta tcattcagcg cctcctttta
      961 aattattcac tttgtggttt ttatcttaat aagcacattt taaaccacga aacgagtaat
     1021 gtcaattaaa acattactca atttgtggtt tttatgctat aatagtttgg aatttactat
     1081 agggggtgtt actttgaata gaataaaatt ttatattgat tccagtaaaa agaccattaa
     1141 acaaattagt acagaaactg gtattaacta tacaactctt acgaactatt accgtggtga
     1201 gcgaaatcct agaaatactg aaacatggaa tatccttgct gattatttcc atgttcctgt
     1261 aagttatttg atgggattag acgaaacaaa ttccatagaa caacctaatc ttgatagaat
     1321 aaaaactgct cgtttagaaa aaaaggacaa aatagtcaag aatattgcca gtcttgactc
     1381 agattatttt actgaattat ctaatctata cttttccatg acttctttac ttgaagtcaa
     1441 acttcaagaa gatgactccg actttctttt tttaaaaagt attctagagg actcctttga
     1501 atacacgatt ctatttgcca aacaaaagaa gtttattaat aaagctttaa acttaaatat
     1561 cgatgaaaga aaagagttag aatctgagtt atttggcaac ttaacccaag tacaggttaa
     1621 caatctaaaa agagagataa cagctcgatt aatatcaaaa cttcaataaa actaattatt
     1681 caatcagcca actcgccaag ttatcagatt ggagagatta gttatgtcaa tacaaaagta
     1741 cacaacaaaa agcggtgcac gatatagtgt ccgcttgtat gttcgtaaag atgaaaatgg
     1801 aaagcaagaa tactatgtaa aaaaagggtt taaaactgaa aaagaagcca agttacacga
     1861 agcaagaaaa aaggtcgaga ttgaagatac cggctatcat aagccttcta atgacttgtt
     1921 tataacagtg tatgaagaat ggttgccttc ttataggaat cgtgtacaag aaacgacata
     1981 tcaacgtaca aaagaccttt tccgcctgca tatactacca aaattcggga ataagtccat
     2041 ttctaagatt actcctgttc actgtcagaa tgcagtcaat gaatgggctt taaaattcaa
     2101 gaatatcaaa cagcttaagt cttacagctc tcaaatattt gagtatgcca tttttgcaga
     2161 gctattacac cgaaatccta tggcaaatac aaatctacca aagcgggaaa agaaagaaaa
     2221 tgaaaattac ttttcattag aagaactaaa agctttttgg gatattctca ataaagaaga
     2281 gcctttaaaa catattttaa tcttccagct tttgattact actggtatac gtaaaggaga
     2341 gctagctgct cttcattggt ctgacattta ttttgaagaa cagctaatgt atgttagaaa
     2401 gagctatgca actattcgaa atgaagagaa gaatgcaaga agaaaaactg tcaggattca
     2461 aaaaaataca aaaaatatat cttccgaacg aatattacca attgatgcac aaactattga
     2521 aatgttaaaa atctggaaaa aagaacaagc tcgagagctt ttgcagtttg gcataaatac
     2581 aaatacaaaa aatcaactta tctttaccta tgtaaatgca gacggtgaca ttaatcaacc
     2641 tttgcatgcg gactattcaa atagtatatt gaaacgttta gagaagaaat ataaatttaa
     2701 gcacgttact attcatggat tccggcacac tcacgctact cttctactag aaggtggagc
     2761 aagtatcaaa gaaacacaag atcgattggg tcataaaaat gctgaaacta cccttaacac
     2821 ttattctcat gttactgaaa aagctcaacg aaatgcgtta gaaaaattca ttgcttacac
     2881 tggtttataa ttttgtcatt acctttctca ttaccagaac gaaaaaaaga caccttctcg
     2941 caatgagaaa gtgtctgaaa tcgttggtat aacaacgatt aacgttttga aaactgtgaa
     3001 gctttacgtg ctttcttaag acctggtttt ttacgttcga ccatacgtgc gtcacgagta
     3061 agtaagccag cacgtttaag agctaaacgg aagtcaggat caacttctag caatgcacga
     3121 gcgataccat gacggatagc tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca
     3181 aatacgtcat atgcgccttt tgtttcagta acagcgaaag gttgattgat tacttcacgc
     3241 aagtcagcat gtggaatgta ttcttcaaca tcttttttat ttacagtaat tttgccagtt
     3301 cctggtacta gacgtacgcg agcaactgcg tttttacgac ggcctgtgcc gatatattga
     3361 acttgtgcca atgaaattcc ctcctattag attaagtttg tgatgtctaa aacttctggt
     3421 tgttgtgctg catgtggatg ttctgctcca ccgtatacgt tcaattttgt aaattgtttg
     3481 cggcctaaag tgtttttagg aagcatacct tttacagaag tttcgatcaa acgacgagaa
     3541 tttttagcac gcaattcgcc agcagtgatt gatttcaatc ctcctgggta gttgctgtga
     3601 cggtaataaa ttttgtcagt cgctttttta cctgttaatt tcactttgtc tgcattgatg
     3661 acgatgacga aatcgccagt atccaaatga ggtgtgaaag ttggtttatt tttaccacgt
     3721 agtacagatg caacaactgt tgaaagacgt cccaatggaa catcagttgc gtctactaca
     3781 taccatttac gttctacttc gcctggtttg gccatatatg ttgtacgcac gttattttcc
     3841 tccaatttaa gttctgatgt ttgacataca cttcgagttt ccggggctct ttgtggggca
     3901 aacaatacca tttaacatat tacccgctaa tctagtcaat gtcaacgttt ttctgcttga
     3961 ttgaaaaatt tatatgaaac tttacttctt ttctttcttt tatataaaag ggtcgtttcc
     4021 tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct tgtcacaacc ccttattttg
     4081 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca taaaacataa
     4141 gaagctgttt tttaaaattg tttcttgtga ctcggagctg aacggcaggc ttacaacctc
     4201 ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg
     4261 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
     4321 ctttttcggt caagttcaac aacagttttt acgcgtcgat ctctggcgct ttgcccaaat
     4381 gagcttggat catccagata cgtttttctg tggctgtttt gaattcgata aagatatctt
     4441 gtgttggatc gtcgccttct tctccagaaa cttcgattcc tttttggtaa agatctgcta
     4501 gataacgata gcctgcaaca agtgtttcca aacgttctgg aatagtacga tcccagcggc
     4561 caatttcatc agggattttt gtgttatctg caaattcacg caatgtagaa taaggtgaac
     4621 cgtctaatgt gatcaagcgt tcggatactt catctaactg agcgttcaaa tcatccatga
     4681 actcatccat cattgggtgc aatgtaagga aacctggtcc tctcatgtac caatgtgttt
     4741 ggtgaacaac aacagacatt tggcttagat ctgcaactaa ttgatttaat acttctttgg
     4801 tttgttggaa tttcatcttt cttgcctcct aacattttct tacacttaaa ataatagtag
     4861 actttcaatg aaaagtacaa caaaatcatt tacctgagaa cgattttcag ttcgcttttt
     4921 tatccgtaaa gccaatcatt tttcagaatc agctaattct tctcgcagtt gagcaaattg
     4981 ctgataagtc tgtgctaaat gcttttgtgt cgaaacaaat aatggataga ttttctgata
     5041 ttttttatga tcagtttctt gtactccaac ttgctctttc aattctatca attcctgtgc
     5101 ttcactgata ctttggatct ttccgatgct tctccagcct aaaatcacag ctcctaatgc
     5161 ggatgcttct gtgacagtag ggaaaacaag cgggtaaccc agtacatcag ccatcatctg
     5221 acgaaaaatc ggagattgag caaacccgcc tgtagcacgg atctctgtga gctgattttc
     5281 ctggcaaact ccttttaaaa tccgataaag gttgagacag atcccttcta gaatcgcctt
     5341 catcatttct ttttctgtat gttgccgctg taaaccgata aaactgccaa acgcatctgc
     5401 gtcccaaagc ggtgctcgct caccaagtaa atagggatga aaaatcagac cattggcacc
     5461 cgctggcaca aaagcgatag ccgaaagcag tgcatcatat ggattttctc ctttttcttt
     5521 agcagattga acgacttcct gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc
     5581 attgctagtc gctcctccca ctacccaatg tttctgatcc agaacataac aaaacgtctc
     5641 catttctgga tggaactgag gctgagtagt aataaaacga agtgctccgc tcgttcctac
     5701 ggtcaaagcg gcgatcgtat caccaaaagc acctaaccct agattagaaa gcggcccgtc
     5761 tgcccctccc aaaacaaatg atatatcatt tggcaaacct agttctgctg ctgtttttga
     5821 tggtagacct gttacttgat aggtggaagg aaagacttgc ggcaattgct ctattcgaat
     5881 gttcaagtag tcaagcactt cttctgccca ttggaattca tggatatcaa aatatccagt
     5941 tcctgaagct gttccgtagt cgctgatcag ttcgccaaac aatctataaa agatatagtc
     6001 tttgataccg acaaaataag cagttttttt gaataattca ggttggctgt tttgcagcca
     6061 cttgattttg ttcaatggtg tcattggatg aatcggcgtc ccagtctgcc gataaaattc
     6121 tttgccaagc acggtatatt tcagtttttc tgcttcgtcc gctgctcggt tatctgccca
     6181 agtgatcatt cgagataacg gtgttttctc ttcatccaac aatatcaggc catgcatcgc
     6241 agaggagaag gagacaaatt gaatgattgt gtctgaaggc aactgtttaa ttacttcttt
     6301 tataacggtg cagacacctt gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt
     6361 ttcttggatc aaagggtagc gaaaataagc ggatgctgct tcttgggctt catctgtgaa
     6421 agcaactgct ttggtttgtg tggttccgat atctaatccg attgaaacgt tcattttttc
     6481 acctcaaatt ttatgtatgt acaaaaattt agtttaagct tgcgtatctt ggttttgaaa
     6541 agggggagat ggcgtgttac tttattttat cattggttgc tgtatcggat catttttatg
     6601 tttaaccgcc cagcggattc cgttaggtca ctctattatt tatcctagat ctcattgtgt
     6661 caaatgctgt cgttcgcttt cttggtatga attgattccg attctctcga tcatcgtcca
     6721 acgatttcgc tgccgctatt gcaggtgtcg gttgcccttc tactatttgt tggcagaagc
     6781 cttatgtggt ggtctttttg cctggttctt tactttttct tccgcaagaa atttttcgac
     6841 gttcatatgg atgctttcgg ctttgctatt ttcgttgatg gatttatttt acttcatggt
     6901 ccattctcac acgctattct gttcttgggc aattttatgg attttttggt tgcaggccgg
     6961 cgtcttccaa tggcaaagtg tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta
     7021 tggtcaatcc tatctcggag ctggcgatac tctgttgatg ctttcttgga gtggtggctt
     7081 gtctctagaa gagttgcttc agatcttgtt tcttgctagt ctgtttggaa tatccgtttt
     7141 ttctgtttac tttatctgtc ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt
     7201 gagcagtgcg ttgctgattg tccttcatct taggtattga aaaaaggttg tgacaaacga
     7261 cttgtcacaa cctctgatat atagtgttct cttaccaaat ccttgtgact tggagctagg
     7321 cgacaggttc acaacctctc cactaaatgg tgttcaatca gctgaccctc agtattagct
     7381 caaaatgctg gttttacaac ctctgatata cggtgttcca taagtagctt tttcggcttc
     7441 ttatttcttg acgccccaat gtcgacgaag gaaacctgca cgtccagaac cttctcgata
     7501 aagattgtaa tggagcttgt ttttcttgta ataatcctga tggtaatctt ctgcaggata
     7561 aaattcgctt cttggttcga tttttgtcac gattggctta tcgaatcgcc cgctgtcttg
     7621 caatgcttgt tttgaacgct ctgcaatatt tcgttcttct tcatcattat agtagatcac
     7681 tggacgataa gaatcaccgc gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg
     7741 ctgccaatag atttccacca actgatcata agaaataact tctggatcat attcgatctc
     7801 tactgcttct gtatgccctg ttgtgcctgt tgttacttgt tcataagttg gatttggtac
     7861 atgcccgcca gtataaccgg atgtcacaga aatgatccct ggctgctctt caaatggttt
     7921 gaccatacac caaaagcatc cgcctgcgaa aactgctttt tttgtcatat aaaatccccc
     7981 ttttttctca tcataccttg atttccaaaa ggaggataac gttttgcttg tgacagaaaa
     8041 aacggaaact attatatacg tgatacggct tctgataaat gccaaaacga acgtacttga
     8101 tcaaaaacgg ttaaatcagg aatcacatat tcagcagaag gaagatttat gttttcacct
     8161 attcccaata catgcatgtt accagccaaa gctgcttgac aaccagcttc tgaatcttca
     8221 atgaccaagc atgcatttgg cggtacattc aattgctgtg ctcctttgag aaaaacttct
     8281 ggatcaggtt ttgcttttga tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc
     8341 aaattcgttt tttctaaaat gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg
     8401 atgtttgttt ttttcaagta ctcaagaaca gctattgcac caggcaaaac ggatgtttca
     8461 tccatttttt gaatcgcttg aagatataga ttgttttttt gctcagcaaa ggctgatttc
     8521 tctttttctg agaatttctg ttccattttt ccaaatgaca gcaatcgctc tagtgactcg
     8581 gttctggata ttcctttcag ttgctcgttt tctttctcag tgaatggaat ggacagctgg
     8641 ttagctaaaa ccagccacgc ttcgtaatga taacgagctg tgtctacaag aacgccatct
     8701 aaatcgaata gtgctgcttt gattttcatc gattacctcc ttgaaaatga acgacttgaa
     8761 gtaaagatgc gatgttttta gctgtgcgat gaagattatc agtggcttgc tgtaaagctt
     8821 cttgtaaagg cacagctgca ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg
     8881 ctttttcgct tcctttacca acacttccgc aaatagctat cacaggaacg ccctctggta
     8941 ctcttctagc aatcccaact ggcgcttttc cttttagact ttgtccatct agacgtcctt
     9001 cgcctacgat tacgaggtct gcttgctgta cccgtttatc gaactccagt aaatccaata
     9061 cgtagtcgat gccagagact attttccctg aagcaaatgc taacagtcct gcacccattc
     9121 caccaccagc actactgccc gatgcctcga ttacttgagg ggcaaatctc atataaaaat
     9181 catgcatggc ttggtcagcc actgctaatt cattttcttt caagcctttt tgtggtccga
     9241 aaacaaaaga agcgccttct tttccgcata aaagattagt aacatcagta atgacagtga
     9301 ttttggcttt aatcagctca ataggcacgt tcgttttatc aactgtagca attttaccta
     9361 gattttctcc cagtgcttct actttttgat tatcttgatc gtaaaaagca taacctagac
     9421 cttctgccat gccgatgccg ccatcgttgg tactacttcc gccaacgccg atgatgatgt
     9481 gtcgaacacc ttgttgcaac aattgtcgta tcagttctcc tactcctcgc gtggataacc
     9541 taagtggctg ttgttggttt agtgggacat ctttcaatcc gacgatttct gccatttcaa
     9601 aaatcgcagt ttgaccgtct gaagcgtact ggatttcttt gtttttgcca aatgcattag
     9661 taacgactgc tgtttgccgc ttcaacctta aactgtctgc taatgatgaa accgttcctt
     9721 ctcctccatc gcctataggt agcagatcat attcagcctg aacaaaaact tcttgaaaac
     9781 ctgattgaat ggcttgtgct gcctctaagg ctgtcagact ttctttgaaa gaatctggcg
     9841 caatcagaat acgcattatt ttcccctccg ttccaagatc agttcttttt ccagctggta
     9901 ttggtctcca ttgacaaaaa taggtaatgt ttctttcgtc tcttttttta ttactatttg
     9961 tttttttgtc aaaacaaata gcaacctgtg atttttccaa ataaaaggaa aagaaatttt
    10021 ttcccatgct gaaggaagtc ttggagaaat agaaagagtt ccattcgtta ctgtaatacc
    10081 tgaaaaacca aatataactg ctagccatag tgctcctaat gaagcagcat gaattccgtc
    10141 atctgatgaa tgcggatctg gtcctaaatc aatcaggcac gcttcttgga ataaatgata
    10201 cgcccattcc atttcttgga tacggcaagc ctctatggcg tgaatcgctt tactcaacga
    10261 tgagtcgtgg attgttttgt tttcatagta atgcaaattc ttagaaacga ttggttgaga
    10321 aaaatactca ggtaataagt ataacagcat gacaagatct gcttgcttgc tgacttggag
    10381 ttcatttact tcgctacggg cataatcaag taaaatagac tggctgccag gacgttcttt
    10441 gtaagtggtc aaatcaataa ctggcttact gagatacgtg tcatcttgcg gaatcagctg
    10501 ttctttgtta ggcactggca aatataactt atctaaaaaa ttttgacatt ctttcaaaaa
    10561 aagcggatcg ccagcttgat atttaacagc taaggccaca ttataatgcg ccagatagtt
    10621 cgtatatgca ttattgtcga tatattctgt gtattcatct ggaccaatga ctctgtgtat
    10681 ctctaaatga tcatcgactt ttacagctct gctcagccaa aaagctgctg tttcatgaat
    10741 gagcgttttt ccctttgttt ctaaaaacga ttgatcctct gttgcttgga catactcttg
    10801 aacggcataa gcaaggtctg cactgatatg atgttctgcg gcagctgaag caacttcttg
    10861 tcttgttccg gttttgatat tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg
    10921 tgcgctttcc catgggaaaa gtgcaccttg atagtgataa tctcttgctt tttgccgtgc
    10981 gccttctaat cgatcataac ggtacagcag caaattcttt gctacttcag gaaactgata
    11041 taaaaagaaa ggcaggataa aaatttccgt atcccagaaa acatgccctt tataaccttc
    11101 gccagttagt cccttcgccc ctacactaac acttggatca ttgcggggag tcataatctg
    11161 taaatggtaa gcagcgaagt ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt
    11221 tatccgatgc tgatgccaaa aatctgtcca ataagacatc gactgctcta aaatttctgt
    11281 ataactaacg cttttctggg cttgggcaat acttttctta aacgggtctg tttgctgaag
    11341 agaggtagag agcacgatga tcttctccag cgtaaaggac gtattttttc tgatcttttc
    11401 tgaaaaggaa gcggtgatcc ggcgattttt tgcttggaat acacctgctt tattgaattt
    11461 agctgcaatc ccaatatcaa ttttactttc tgttgtctgg taggatgcac tgatcaaatc
    11521 gtcaccaaat actcgcagtt ctttttcaac caactgctgt gtaccaaaat tggtttgctg
    11581 tccatcgata cccgttttca catctacttg gcagtcttcg gtcaacggct tgatgaccac
    11641 tttcagggct gcctgatgga gattattttg atcagcaaca cgacaagtaa tgacttccac
    11701 tttttttcct tgagcagttt caatgagcgc atagcgttta agctctccca tttctaaatc
    11761 tagtgcccgc tcgtatgcaa gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat
    11821 ttttatttcc catctgatga catctgggag attcactaac tcggctgatt ctgctcctaa
    11881 aggtcggtta tatacaccag ctgcaaacat tccccttact tgattccgat agtcttcctc
    11941 ctggcttcct ctgataccaa aaaatccatt tccaatggtc atctgactcg catacttatt
    12001 gatatactct aaatcaaact gtttttcttc tactttcata aggtaataac ttctcctgtt
    12061 tctgccgaac gatataaatt ttccaccaga ttctggatga catatccttg ccagccatct
    12121 gcaatcatct gagtctggtc accgagcgcc cgattcacaa aatgagccat acttagttca
    12181 tgtgggtcat taactggcgt ggtcttttgt aaggaaacca gctttccatc tacatcttcg
    12241 aatagttcgc ctggatacag attagcacct gcctgatcgc cacaaaaagc cacattcagt
    12301 ttgtcttctc tgatattcaa agcaaacgaa gtattcagtt ggatgatctt gccgttttct
    12361 aattccagtg tgccaaaaat tgcatcttct actgtgaatt tgcttggatc ccatttcccg
    12421 aatttccctg aatttttttt cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt
    12481 actttaggaa atccagtaat atacatcgct gtatcaagca tgtgaatgcc ataatcgatc
    12541 aaaggcccgc ctccttgaag ttctttgtcg ataaacgttc cccagcctgg aacaccagac
    12601 cgacgaagcg cattcgcttc tacgtaataa atatccccga gaacatcaag atgattcttg
    12661 atcattgttg cttcgtcaga aaaacgatgc tggaatccat aagccaaaat ctgcccgttt
    12721 ttatcggctg tctctgccat ctttcttgct tgatcagctg tcatagctgg tggtttctcg
    12781 cacatcactg aagcattagc ttcgagggca gccaatacat gttcgaagtg aaagcgattg
    12841 ggtgtacaga cactcacgat atcgggagaa atctcattca gcatttgtgt aactgatgta
    12901 tacgcatgag aaaatcctgt acgtcggcaa aagtcttctg ctcgctggct gactggatca
    12961 accacagcgg ccaattctaa gacatctgat cttttttgat aataagcagc atgtactttt
    13021 tctgctactt gtccagcgcc tatgattgct actttcttca tttatttctc tctcctttgc
    13081 ttatttactt attgttattc tttctctatt ctttctctaa atcatttaaa tgacgcgctg
    13141 tttccagaca gtttttcaag tattgcaatg attcctgata aattttcact tcattttctc
    13201 ctcgcaaacg acattcatat accatataac cttcatactc attttgaagt agcgtttgaa
    13261 aatggcgagt aaaatcaatt gcgccagatc ctggttggta gcgatgatta tctgctaaat
    13321 ggatatgacc cacatactct ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa
    13381 tattcatatg ataaaaatca gcgatgatct gcacatgttt caattgatta gagtcaatgt
    13441 aatgacgcgc atccgctaac aaattgatca tatggtcttg gtatcggttc aaaggctcca
    13501 aataaataac cgtacctgtt tcttcggcta ctttatctaa ataacgaagt gattcgctga
    13561 ctgctttata gtctccttct ttgcttcggg gtgaaaccat cggcggtaat cgataagtga
    13621 acattcccca agctgctggg ataacgattc ctttgcctcc aacctcgctt agtgcacgca
    13681 aaatctcagc aatttgacgt acaccattaa gacgccgttc ttcgataaag tcaccgatcc
    13741 acccatcgta gccgttacaa gctgtcacta ctgggatccc tgtttctttg attgcctgtt
    13801 tgactgtgtc gatattttct attaacaact taccgtcgat ttcgtattct tcaaatccac
    13861 agtctttgat aaatctaaat ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg
    13921 taccgaattt cattgttctc ctcctcggac ttgaacgccc attttgatac tttcttctgg
    13981 gtgctggtct acataacgca tgtaagcttc tgcgctttct gcaaatggca caactggatc
    14041 aatgatttct tcacaatcca agtaaccgtt catcaataat tcccaacatg tgtcctcgat
    14101 tcgtttacgg ttccatcttg ggtaatctgg gtttggttca ctagatgctc gagcaaagac
    14161 gatttttgca ttattgaaat gtgcttcgcg accaagatta aacccagctg gaaaaggttt
    14221 agcaaaggca acataagaaa tgatcccgcc atagccgatc cctattaaag aagcttgtaa
    14281 tgcggcagca ctgccactcg tttcaataac tgaatctact ccacgtttat ctgtcaattc
    14341 ctttagacga aaaccgacat ctgctttcat aggatctagc gtgtaatcgg caccgtgttt
    14401 ttctgctaat tctctacgct gcgctatggg atcgatccca acgatggtag tagcacctgc
    14461 ttttttagct aattgaatcg caatttgccc gatagcgcct aaaccgaaga ttgctacagc
    14521 atcacctgct cgaacatgtg cttcccggat accacctaaa gcgaattgag caggatcgta
    14581 acatactgca tttttccatt gatccaaccg atcaagttta cgtagtttat aattatcaac
    14641 accgtttgta atcagcgttt cacgaatggg tccataagtg cagactaact ctcctttttg
    14701 atactctgtc accaaactgc ccgtttcttc gatcgtaccg acaatcatat ttcctaattg
    14761 aaattcgcca aataccacac cgcgttggct tccttcttct cttggcaaga ataggcgcca
    14821 ttcttctgaa aattcttcat cgataaatgg acttaatcct ctaaagtcga caatttctgt
    14881 tcctggtttt ggagtagcga aaattactcg gatacgtact tcgttctttt cgatctcacg
    14941 gtcttcgtaa tctttgaatc cggctattct tggttctaat gcaactaatt ttttcatctt
    15001 ttctttcctc tttcttttct ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa
    15061 taaatcgttc agataacgca tacataatga tgactggtaa agcggtcacc aatgaagcgg
    15121 ccatcattcg tccccaaatg tagtctggtg tactgaataa tgtgttcaaa ccgattggta
    15181 aagtaaattt ttcggaggtc gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa
    15241 ccataaagca ataaacgaag acagaaatga ttccagaaat agccaatggt ataatgatcc
    15301 gaataatgat ctggatccgg ctaagaccat cgatcattgc tgcttcttca atatcagtcg
    15361 gaatcgtgtc aaaataactt tttagcatga atatcgcagt aggaagtgtt tgaacgacca
    15421 tcgtgataat cagtgctgta ttggtatcat acaaacctag accggaaatg attttaaata
    15481 atggaacaac gatcaaaata ccggaaaaca tataaacggt gtaaaagcta gcattgattt
    15541 tcgagcgagc tttgaagcgt agttttgaca aagcgtatgc cccgctgatc ccaatcacta
    15601 ctgcagttac ggaggctatc aaggagatca ctaaactatt tttgaaatac atgatgaatg
    15661 gaaaaatctc agcattgaaa atatcctgat aatgttcaaa agtccattct ttcggaaaaa
    15721 tagtttgtgt cgttgcaatt gcttcttgtg aatccttgaa tgatgtcatc agcatgatca
    15781 aaaatggaaa caaattgaca gctaatagca acaaaatcgt cgcgtaaaat aagattttcc
    15841 tgctcttttt atttttcatt tcaagtccac cttctttcgt gagagcaaga cgaccgctgc
    15901 aataatgatg aacaatagaa tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa
    15961 ggctgtctga tacaaataaa cgcctaagat attgacttta ttcgtcagta gatagacatc
    16021 tgtaaacata tagaacatcc agatagatcg taaaacaatc acagttgcta gtactggttt
    16081 gattgccggc agtgtcacga tcttgaactt ctgccaagca tttgcaccgt ccatttcagc
    16141 tgcttcataa agcgttttgt cgactgtttg caaaatagcc aaaaatgaaa taaacgcata
    16201 cggatagtat ctccagatag caaacaaaac gaccataaag aagctagcaa ctggctggtc
    16261 gaaccacagc ggtgcttcgt tgaaaagctg caacctatct actaacagat cattgacgat
    16321 tccataacta ttattgaaca tatatttcca agcaaaaatc aaagaaatag atggcgtaac
    16381 ataacttaat aaaatcagtg aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa
    16441 aatagctacg gctaatccta aagctgtact gcctgctaca actaagaatg tgtaagcaac
    16501 tgttaaacct aaagaacgat aaaattcact atcacttaaa atttcaagat aattgcttaa
    16561 tccgataaaa acggcgtctg agttaggatt cagcggcata tcgaaaaaac tgatctgaat
    16621 attggaaagc attggaatca gcactaaaac agctaataaa agcatgcttg gtgctaaaag
    16681 tcccatggct aattttaagt cagaatgttt ccttggacga ttcattgaaa cctccccctg
    16741 ataatctgcc aatagctatc tacttttttc gaacaccatt ggcagaactt cttgttattt
    16801 cgtatttttt tgtgcgtcag ctaaagcttg atcgacatct tcgtttccga cagttacttg
    16861 gttcactgct tttccgatca cacctgagct tgtgatatct cccattttcg tgaaattctt
    16921 ttccccgact agtccaaaga cttgtacttc atcaaaagca ctagcaatct cagatggcag
    16981 ctcgccaaaa gacttgatga cttcattttc tttgtaacca tccagttcga ctacttgttt
    17041 attcactggt tgagcaccac caggagacat caagacccac ttttccatgt ttttcggttg
    17101 agataagaaa gctgtgaatg cttcagcagc ctctttttgt ttctcgtcta gaccagaaga
    17161 aatcgtcaat ccagatactg tgccatagac agcttgatct ttttgtgttg ggatagcaaa
    17221 accgatattt tctgagttcc ccgcttcata aacagaaggc aaaatgtaag ttgaatagat
    17281 tgccattggt gctgttccat tcatgaaagc atctttgatt tctgtcacat cattagagcc
    17341 tggcatcgtg tattgggata agttttgata aaacgacaat gcttctctca tcttttccgt
    17401 atcgatcgtt acctcgcctt catcatccaa aacatttgct ccatttgaca aagcaaattg
    17461 agagaacgct tgctcactca ttgttccttc agcagtaggc atagcaattc catattgttt
    17521 attctcttta tctgtgaaat gttgagcaat ttttaaaata tcatcccaat tttctggttc
    17581 tgaaaaacca gcttctgata atttttcttt gtcataccaa ataccttgaa cccagccgct
    17641 aattggtgca gcaatatagc tttttccatc ttctgaacga acaaggtttt tggctccgtc
    17701 ataataatta tcttctccag cttcctcgat aacgttttgg actgccgtct gatcaatcag
    17761 ttcatcttta tccatcactt tagcaaaatc ctgactgact tccattactt ctggtaattt
    17821 accggaacga gctaacgtaa cgatttttgt attgaacgct gattcttcta ccggcacttg
    17881 cttgattttg atgtctggat tttctttttc aaaatcagca accagttcat tgatcacact
    17941 taatcgttcc tgttcgacag aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt
    18001 agagctttga ctttcaccac aagcagtaag aagaaatcca gtagctagca gacaggtagt
    18061 aacaataatt tttttcattt ttttccacct cttattttat tattttcagc cacatatatt
    18121 catacggctg gaatatcaac tttcgttctt gactgcaatg gataatttgt ccagaaaaca
    18181 ggtctaggta atctcctttt ggtaaaagat aacctgctga ttgattggaa aaattatgga
    18241 taactaagac tttctcttcc ttcgcttcgc gaataaaact gataaaagaa tctgctgagt
    18301 ccagtaagta aatcaaacta ttcggatgga aggctttttc tttttttcga atagatagca
    18361 tctgggtcaa ttcaccatat actgctgttc ttaatggatc tgtctgcaac tctttctcaa
    18421 tatctgctaa caaaaatttt tgccgattga tttttcgatt ctctcctgtt ttttctacgc
    18481 ctttgtaatc atttcgactt cccagcacac tttgaatata aattgccggc acgcctggta
    18541 acgttaccaa aaatgaatga gcagctaaaa agcgtttgct tctccattcc gtcgtatctg
    18601 ttggagaact taacgcatct agatacgtag cgtttatctc atatggactg gtcgttccat
    18661 ctgggttttt cttgtagttt actcgggcac cctctttctt cagctgttcc actaattcaa
    18721 ggatcgtttt ttccggcaga atacctcgta ccggattcaa tccgataccg tcatgggagg
    18781 ctaaaaaatt aaaaaaagtt gtttttttcg atgcgaaatc gatggttttt aaccattctt
    18841 tcaacttact tgtattaccc gaacgaatcg cttgaagggt caaaggtggt agcgaaaact
    18901 gatagaccat ctgtgcttca ttctcgccat tcccaaaata actgatatta tctttgtgtg
    18961 gcacatttgt ctcagtgatc aataccgttc ctggagctgc ttcatcagcg attgcacgga
    19021 ataattggat gattttatgc gtttctggca ggtgtataca agaagttcct agttctttcc
    19081 ataaaaatcc cacagcgtcc aagcgaataa atgaagcgcc tttttctaag taaaacaaaa
    19141 gaatatcgat cattttcaat aaaacttctg gatttttaaa attcaagtct acttgatctt
    19201 cgctaaaagt cgtccatacg tgtttttctt tcccgttttt gtcaatgaac gttgtaagta
    19261 acgggctggt ccttggtcta gtcacgctgc ttgtatctgt atccagtggc aaatcaataa
    19321 aaaaatcttt gtacgtatca ttcaagttta aatattcctg aaaccaagta ctttcggcag
    19381 acatatgatt gcagacaaag tcgaacatta accgtgtttc gcttctcatt tcttctatat
    19441 cttcccatgt gcccaaacgg tcgttgattg cctgataatt tacaacggag aaaccatcat
    19501 cagatgtata tgggaaaaat ggcaagaaat gtacaactct aaatgtatct gccaaatact
    19561 tttgataaaa tttcttaaat gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc
    19621 cataagtaat caagaaaata tcttcttcgt cccaatgcag tttgcctgcc tggcctttct
    19681 tctgctgata aacactgact ttttgttcaa tcatagaggc ggttttctca gacaaatctg
    19741 agtcgcgata aatcgcttgt aaaagttttg tatacatcgg attccccttt cttttttggg
    19801 agcgttccca acgatttgat catattatgg aaaacgctta ctgtcaatag cattttttta
    19861 aataattttg atatacacaa aaatacaaat aagaaagctt atgtatcaat aattaatagc
    19921 ttcttaaaaa aatatggtct ctcatgatca tgcaagctat tcctaaattt tcaaaatcat
    19981 agacaaaaaa gaatgtctat tgtactatgg aattattcta aaacgctccc aaaaaggagg
    20041 tgcatataaa attgaaacct actatttatg acattgcgaa aaaagctaat gtctcaaaat
    20101 caactgtctc cagagtatta aataaccagc caaatatctc tgataaagca aaagcaagtg
    20161 tacttcaagc aatcaaagaa ttgaattatg aaccgagcaa aattgcaaga ggactgtcag
    20221 gaaatggttt tgacgcgatt ctagcattat ctcatcgtac ttctcatacg acctatggca
    20281 atcctttttt ttcagatgtt attcaatcca tttctgccgt ttctgaacaa aataacttcg
    20341 acttgattct acaaacggca aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga
    20401 tccaagaaaa aatgatccga ggaattatta ttttaagctc tacgatggat gaaacacttc
    20461 tttttgaatt ggatcaatac aatattccta ttgtggtaat cggaaaagtc ggaaacgact
    20521 acacgaacat ctactcagtg gacacggata atttccagga tagttacgat ttggtggaaa
    20581 cactgatcaa gaatggacac cgtcatatcg gctgtctcca tgctccgaca gaagtttatg
    20641 tatccaatga tcgtctcaat ggttaccgtc aatgcttatt tgatcaccac ctagatatcc
    20701 gtaatgaatg gattatagac ggcggctttc gcttagaaga tagcatggca gcaatgaatg
    20761 atttaatgaa gctacgagaa agaccaactg cgattttttc taccgatgca ttaaaatcat
    20821 tgagtattta caaagtggca gaacaatctg gaaagcagat acctgatgat ctttcgatcg
    20881 caggattcag tgatccctta ttgtcctccc tattttcgcc acagttgact cggatagaca
    20941 ttccgacaaa tcagttagga atcaaagcca cagaactttt attccaacta atccaccacc
    21001 aataccctga agaaaaaaga atcattcttt caaccgagat tactctcaca gattcgatcc
    21061 aacaacaaga aatgtagaac agaattataa aagaacagaa acactgagga taagtcagtt
    21121 actctgttct tttttcaatc actcatccct cgttaccccc tcccggtatt ttagcgaatt
    21181 tttcttattt tccatctgtt ttagatagct gtggataact ataatgtttg tgttaaacta
    21241 ttttttccac agggaagaaa aaactatttt taaccacgtt gtgtataagt ttttatagtt
    21301 cgttttttta aacacattta cccttatttc aatccatgtt atttggggat acttatctct
    21361 aaaacataaa tagaataaca gacatgtcta ctggttttgt tttattcgag gaattattca
    21421 ataatagcta gcaaaatatt aaaaataacc gctctatctt cccatcaatt tattttgcat
    21481 tcaaaaaatg atctatcttt acttttgttg attgacttct cttggaagat gatcgttccg
    21541 ataccttaat tggatacgaa ccgatgttgt taagatcctt tcccactttt cttctagtta
    21601 gacaaattat tttagacaaa ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt
    21661 ttttaaaata tctattttgt ttgattgttc attgcgtctt ctgcagccat ttgcgcttca
    21721 atatctgaaa tactgtagac attttcgctt gctacaccga cttcttttgg ttccatgtca
    21781 cgataacgtt gcataccagt accagctggg atgatcttac ctataataac attttctttc
    21841 aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt
    21901 tcttggaatg atgcagcaga taagaaacta tttgtttcta atgatgcttt tgtgatacct
    21961 agtaagacag gtcgagaagt tgcaggaacg cctcctgaga ctagcgtatt gtagttttga
    22021 tctttaaagt ctgcgatgtc taataatgta cctggtaaga tatctgtatc acctggatcc
    22081 attacacgaa ctttacgcag catttgacga accattactt cgatatgctt gtcgccgatt
    22141 tctacccctt gcatacggta aacacgttgt acttcacgaa gcaagtagtt ttcaacagat
    22201 aatacatcgc gaacttgcaa caattgtttt ggatcgattg atccttctgt caatggtgca
    22261 ccacggtgga tcatatcgcc ttctgctact ttcatacgtg ctgtataagg aacagtatac
    22321 gtacgtgtat ctgtttttcc tttgattgtt acttctttgg tacgtgttgc aggatcttct
    22381 gagatatcga tgacatcccc tgttacttct gtgatcactg cttgcccttt cggattacgt
    22441 gcttcaaaga tttcttggac acgaggaaga ccttgagtga tatcgtctcc ggcaacccca
    22501 cctgtatgga atgtacgcat cgtcaactga gtaccaggtt caccgattga ttgagcggcg
    22561 atcgtaccaa ctgcttctcc gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa
    22621 caatgtttgc agacaccatg ttttgtatta catgtgaaga cagaacggat tgtcactgtt
    22681 tcgacacctg catcaacgat ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca
    22741 atgatcaatt catttgtttc aggatgacgt acttctttac gggtgtaacg tcccactaaa
    22801 cgatcttcta atgattcgat gatttcattt ccctcgcgga tggcttggat ttcaagacct
    22861 cggtcagtac cgcaatcttc ttcacggatg attacatctt gggccacgtc aaccagacga
    22921 cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat cggtcatccc tttacgagcc
    22981 ccgtgagttg agataaacat ttccaagacg gatagtcctt cacggaagtt cgaaatgatt
    23041 ggcaattcca tgattcgacc attaggagcg gccatcaaac cacgcattcc ggcaagctgt
    23101 gtaaagttgg aaatgttacc acgagctcca gaatcagaca tcatgaagat tggatttttc
    23161 gcttccaaac cttccatcag tttttgttgg atgctgtctt ttgcagcatt ccaaacagca
    23221 atgacacgtt cataacgttc gtcatctgtg atcagaccac gacggaattg ttttgtaata
    23281 gtttcaactt gtttatgggc attttcaatg atttcttgtt tttcattcag tacactgatg
    23341 tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt tgtaccctaa gtctttcata
    23401 cggtcaagca tcttagaagt ttctgttact ttgaatcgtt tgaagacttc tgcgatgatg
    23461 tttcctaagt ttttcttctt gaacggtaat actaattctt gctctttgat atgtgcagga
    23521 atatcagtac cagcttcaac aaagtatttg tccggtgttt ggacggtcaa gttgaagtct
    23581 gttggttcat tcagataagg gaattcagga ggcatgatct cgttgaagat gatcttacca
    23641 actgttgtga tcatcgtacg ttctttttgc cattcagtaa atggtttttc acctaatgag
    23701 ttagggttca caccgatacg tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg
    23761 gcttcgtttt gatctctgaa gaccattcct tcgccttcgc gtccttcttc ttccattgtc
    23821 aagtagtagt tacctagaac catatcttga gaaggagtaa caactggttt accatcttta
    23881 gggttcaaga tgttttgagc agctaacatc aacatacgtg cttctgcttg tgcttcttca
    23941 ttcaatggaa cgtggacagc catttggtct ccatcgaaat cggcattata agcttcacat
    24001 acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata
    24061 ccaagtcggt gaagcgtagg tgcccggttc agcaatactg ggtgttcctt gattacgtct
    24121 tctaaaacat cccatacttc gtcttcttga cgttcgattt tacgtttcgc atttttgatg
    24181 tttgaagcta gttcgcgttg aacaagctca cgcatcacga atggtttgaa taattcaatg
    24241 gccatttctt ttggaagtcc acattggtac atcttcaaga aaggtccaac aacgataacg
    24301 gaacgacctg agtagtcgac acgtttacct agcaagtttt gacggaaacg accttgtttc
    24361 cctttcaaca tatgagaaag agatttcaat gggcggttac ctggtccagt aactggacgt
    24421 ccgcgacgac cgttatcgat caatgcatct actgcttctt gcagcatacg tttttcattt
    24481 tgaacgatga ttccaggtgc attcaagtct aacaaacgtt tcaaacggtt attacggttg
    24541 attacacgac ggtaaagatc attcaagtca cttgttgcaa aacgtccacc ttctaattga
    24601 accattgggc gtaaatctgg tggaataaca gggataacgt ccataaccat ccagcttggt
    24661 tgatttcctg aagcgcggaa tgcttctaaa atatccaaac ggcggattgc acgtgtacgt
    24721 ttttgtcctt gtgcagattt caattcttct ttcaattcgg ctgcttctgc atctagatca
    24781 acattatcaa gtaattgttt aatcgcttct gcacccatag cagcttggaa ttcttgtcca
    24841 tattgttcac gtttttcgcg atattcgcgt tctgtcaaca attgtttttt ctctaaattc
    24901 gtatttcctg gttcgatgac tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt
    24961 gggctcatgt ctaatacaag acccatacgt gaaggaatcc ctttaaagta ccagatatgt
    25021 gaaacaggag ctgctaattc aatatgtccc atgcgttcac ggcgaacttt cgaacgagta
    25081 acttccactc cgcatcgatc acaaacgatt cctttataac ggatacgttt gtattttccg
    25141 caggcacatt cccagtcttt tgtaggacca aaaatccgct cacagaacaa cccttcgcgt
    25201 tcaggtttta aggtacgata gttaatcgtc tcaggttttt ttacttcacc gtaagaccag
    25261 cttctgattt tttcgggaga agccaaacct atttgcatac tttcgaattt atttacatcg
    25321 atcaaaaggg gttccctcca tttcatttaa tggcaaaccg caagacggct gatcttcgac
    25381 cgccttgcag ctcatagacc ggattagtct aaattatctt ctgcggtttc aaagttttgg
    25441 atgacatcgt ctttttcatc ttccacttgt tcagcagctt tcttttctaa ttctttggca
    25501 gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg
    25561 tccatgtcgc gaagttcgat ctctgtttct tcgatatcca agacacgcat gtccagacct
    25621 agtgattgca attctttcac caatacgcgg aatgattccg gtacacctgg ttttggaatt
    25681 ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg
    25741 tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag cttccagtgc ccatacttcc
    25801 atttcaccaa aacgttgtcc accaaattga gctttacctc ccaatggttg ttgtgtaacc
    25861 aatgagtaag gtccgattga acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg
    25921 atcatgtaca tcacaccgac agacacacgt ccatcaaatg gttcacctgt acgtccgtcg
    25981 tacagaacag ttttcgcatc gctggccata ccggcttcac gaactgtttc ccatacgtct
    26041 tcatcacttg caccatcaaa tactggcgtt gctacgtgga tacctaactg acgtgcagcc
    26101 atacccaagt gtaattccaa tacttgcccg atgttcatac gtgaaggcac ccctaaagga
    26161 ttcagcatga tatcgattgg tgttccatca ggtaggaatg gcatatcttc ttccggcata
    26221 atacgggata caaccccttt atttccgtga cgaccggcca ttttatcccc ttcatggatt
    26281 ttacgttttt gaacgatata aacacgaact aacatgttta cgcctggtga caattcatct
    26341 ccagcttcac gagtaaagat tttcacatcg tgaacgatac cgccgccgcc atgtggtaca
    26401 cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa agatcgcatg caatagacgt
    26461 tcttctgcag ataattctgt tacccctttt ggcgtgactt tccctacaag caaatcgcca
    26521 tcttggactt ctgcaccaat acggatgata cccatttcgt ctaaatcttt taatgcgtct
    26581 tccccgacat tagggatttc gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct
    26641 tctgattcat attcttcaat atggatagaa gtgtatacgt catcttttac caaacgacgg
    26701 ctcatgatga tcgcatcttc atagttgtac ccttcccaag tcatgaaacc aactaaaacg
    26761 ttttggccta gagccatttc gccttgttcc atagatggtc cgtctgccag agtatcccct
    26821 ttttcgactt tctcacccaa atgaacgatt ggacgttggt tgtagcttgt tccggagttt
    26881 gaacgacgga attttgttac atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg
    26941 atttcagaag catcaacgta ttcaacgaca ccgtcatgtt tacataacaa agcagctcct
    27001 gagtcatgcg ctgatttgta ttccatacca gtaccaaccc atggtgcttg aggattgatc
    27061 aacggcactg cttgacgctg catgttggca cccatcaagg cacggttgga gtcatcgttt
    27121 tccaagaaag gaatacatgc tgtcgcaacg gcaactactt gtttaggtga tacgtccatg
    27181 tagtctactt tatcaataga tacttctaag ttttcacttt gagcacgagc cataacaacg
    27241 tcttgagcaa atgttccatc ttcattcaat gggctgtttg cttgggctac gatgtaatgg
    27301 tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct
    27361 acacgacgat aaggtgtttc gataaatccg aatttattta ctttcgcata actggacaaa
    27421 ctattgatca acccgatgtt tgggccttca ggagtttcga tcggacacat acggccatag
    27481 tgggagtagt gaacgtcacg aacttcataa ccggcgcggt cacgagtcaa tccgccaggt
    27541 cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg
    27601 aactgtgaca actgagaaga accaaagaat tctttgatac ttgccacaac tggacggata
    27661 ttgatcaatt gttgaggtgt caatgtttca gtatcttgga tcgacattct ttcacgaacc
    27721 acacgttcca tacgtgctag cccgatacgg aattggtttt gtaaaagttc accgactgaa
    27781 cggatacgac gatttcctaa gtggtcgata tcatcgacat tgccgatacc ttccatcaag
    27841 ttcaagaaat agctcattga agcaatgatg tcggctggac gaaccgtttt tacagctgct
    27901 tctggatagc cgtttccgat cacgtttact tcacgttcag ggtctcttgg tgaaaatact
    27961 ttgatgactt ggacagtcat tggatctgtg accacaccgt cttctgatgg atagtaagtc
    28021 actgaattta ggccattatc aatgaaagga gctagtgttt ccatgacttg gtgagtcaga
    28081 actgttcctt tttcaacgat gatctcgcct gtttctggat caactaatgt ttcagctaat
    28141 gtcagattca atagacgtgt tttcaaatct aattttttgt ttactttgta acgaccaacg
    28201 tttgctaagt cgtaacgttt tggatcgaag aaacgagcat tcagcaagtt acgtgagctg
    28261 tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct
    28321 gttcttgagt cgcttgcgtt tttatgcaag tctttttcga ctgtattgcg taatgtttcg
    28381 ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa atcctaacgc acgaactaat
    28441 acggttaaag gaatcttacg tgtacggtcg atacgtacat aagagatgtc ttttgcatca
    28501 gtttccattt ctagccaagc tccacggtta gggataacag tagaaccgaa tccttctttt
    28561 ccgtttttgt cgacttttcc atggaaatag acacctggtg aacggactaa ctgagaaacg
    28621 attactcgct ctgccccgtt gatgatgaat gttccttgtt ctgtcattaa tgggaaatca
    28681 ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt
    28741 acatgtaatg gtgcagagta gtttgcatca tgcgcacgag cttctgctac tgtatacttt
    28801 ggttctttca attcataatc tacaaattcc aaagatagat tgccattgaa gtcatcgatt
    28861 ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta agaaccattg gtaagagtct
    28921 gtttgaattt cgatcaaatt tggtaattcc aacacttcac tgattcttgc gaaacttcta
    28981 cgttcgcgat gttttccgta ttttactacg tgtccagcca agctcttcac ccctctaaaa
    29041 tgtcattcaa aaagactacc cgatcaattg ttacaataat atttcaaata ttatttttag
    29101 gtaacaacag acattttttg cggcttttag gcaaaaaaaa accaaaaata gaacattctc
    29161 ttgaaacctt acttcgtgag cttctacttt tgtttcctta tattaaagcc gaaacggaca
    29221 atatttcgtt tctgcctttt cttcatcaga tagtttttgc aacttcatat actacactta
    29281 ttttctataa aagtcaacac ttcctggcgt ttcttctgct ttttcgcaac attttcttaa
    29341 tgttttcaat tttatgtttt caattatctg acaattctta attgacagac ctttttatct
    29401 cggttatact ttagaaaatt cattttgtgg aggaacttct atgcgaacca acatcaacca
    29461 aatcgcacca acacaactga ataactttta ctttagctac tttagatagg tttgtattca
    29521 tcatgatgga tacaaacgtt aaacgacagt ttgtatctat gatggctaaa gaactttgta
    29581 ttgcagcaaa aagccacgta gacaaaatgc taagtggctg agcagtgaaa atacaaagta
    29641 aaagtatcaa gcattctact ttttcaattt attttccgac ttttcaatcc atttggacat
    29701 tttgtctgaa tggatttttt gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat
    29761 ataatcatcg gattaggcgc tgccctgcta ctagctttag gaggttgcgc acaaaaagac
    29821 aattccgcag aggcatcaga tggacaaaaa acaattggcg tattgcaact tgtacaacat
    29881 aactcgttag atgctgccta taaaggattc aaagaaggat tagcagaaga agggtataaa
    29941 gaaggcgata atctaacaat cgattatcaa aatgcccaaa acaatcaaga caacctgaaa
    30001 agtatgagtg agaaactcgt caaagaagat cctgatttgc tacttggaat cgccactcca
    30061 gcagcacaat ctttattaaa tgaaacaacc actatcccga tgactgtcac agcggtcaca
    30121 gacttggaac aagcccattt agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc
    30181 acgacagata tggtgccaat cgaaaaacaa atcggcttac tgttaaatat cgtgccagac
    30241 gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa actctaagat ccaagctgat
    30301 ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca aagtactgac agcaaattcc
    30361 acaaatgatg tccaacaagt gacgaaaagt ctagctaaag atgtagatgg aatctatatt
    30421 cctactgata acacgttcgc aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca
    30481 aaaacacctg tcgttgctgg cgcagttgat caagtcaaag acggcggtct agcaacagta
    30541 ggaatcgact acgaaagctt gggcaaacaa acagggatca tggcagcaaa aatcttagac
    30601 ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg atttggaact gtatgtcaat
    30661 aaagacatgg ccaaagcatt aggcatcaat ccagacagca tcaaagaagc tgaataaaac
    30721 acaggaggat tctcatgaca aatttgctct ctcttttctt atcatctgca tcgcaagggg
    30781 tcttatgggc cctacttgcg attggtgtat acgtcacttt ccgtatcttg gacattgccg
    30841 acttgactgc tgaaggaagc tttcctttag gtgcagggat cgcggcagta agtattacaa
    30901 atggctatca tccattgaca gcttgtcttc taggcttttt agggggtgcg gcagcagggc
    30961 ttgtttccgg actgcttcac acaaaattga aaattcccgc ccttcttgca gggatcatca
    31021 ccatgactgg tttatattct gtcacatccc gtgtgatggg tgcgccgaac atctctttat
    31081 tgggtcagcc aacgatcttt cattgggtcg agtcattcgg cataacaaaa gacaacgctg
    31141 tactgatcgt cggtctgctt tttgctttga tcgttgtatg tttacttgtt ttgttcttac
    31201 gtacagaaac aggacttgct attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa
    31261 acgggatcaa cacagataat atgaagatca tcggttatat gatttctaat ggatgcattg
    31321 ctctttctgg ctccttactt gcgcaaaaca acggctttgc tgatttaaat tctggtattg
    31381 gaacgatcgt aattggactt gcttctatta tcatcgcaga agttttgtta cgtaaccagc
    31441 cactatttct ccgtctcttg actgtcgttt taggtgcggt catttatcgt tttattttag
    31501 cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc
    31561 tagtgatctg tctttctttc ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga
    31621 aaggcggtag tgtcaaatga atgacgtctt aacattggaa aatctccatc atacctttga
    31681 aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt aatctttcct tagatcccgg
    31741 cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa tctactttgc tgaacagcat
    31801 cgccggtaca ttttctgtcg atcagggtca aattttattg aatggaaaaa atatcacgaa
    31861 aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta ttccaagatc ctaaactagg
    31921 gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa
    31981 acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca tttttcaaag accagctggt
    32041 tcggctaaat ttggggttgg aaaatcgttt atcctcagaa atcggtttgc tgtctggagg
    32101 acaacgtcaa gctattacgt tattgatggc cacattacag cgtccagaac tgatcttatt
    32161 agatgaacac actgcagcgc ttgaccctgc tacttccaaa acggtgatgg aattgacaga
    32221 aaagctgatc actgagcaga atctcactgc ttttatggtc actcatgaca tggaagacgc
    32281 gatccgctat gggagtcgac tgatcatgct gcatcaagga aggatcgtcg tagatatcca
    32341 gcaagaggaa aaacagcagc tcacagtgcc ccaattatta gcgttgttca aacaaaacag
    32401 cggcacggca ttaaaagacg atcaagtact tttatcttca taatcagaaa gaggatggga
    32461 caaaaaatga acgcccaccc tcttcttttt tctctttcct tctttcgttt ttgctttatg
    32521 tatcaaccgt tgtttcttcg aaaactattt tgttctctcc ttcttcctga tagaatggat
    32581 gggaaaggag cttgatgcca tgacaacgaa agacaaggta cttcaattat tgaaaaagac
    32641 accagatttt ctttccggag aaaagttggc acagcaactg aaagtttccc ggacaagtat
    32701 ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt tttgagcacg ggcctacagg
    32761 gtatcgttac ctgccctctg acgtactaga tgcaaaagaa atccagcaag agctacggtc
    32821 gtttattcct gatttgaaag tacaaacgat gttgacttct gaatctacga tgaaagatgc
    32881 caaactggca gcagctcaag gagattctgt ttcgaagtta tttgttgctg atacacaaga
    32941 acaagctcgt ggacgttttg gacggccttt tttcgctgaa cctggacgag gaatctacat
    33001 gagtctcttg ctccatccag acaaacggtt tgatgaattg cctcaatata cgatcatcgc
    33061 agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg
    33121 ggtcaatgat atctacatgg atggaaaaaa agtctgtggg attttatctg aagcagtcag
    33181 tgatatggaa agcggcagga tcagtcatat catcattggg atcgggctta atttttctgt
    33241 tcctcaaaat cattttccag ctgatctcca agaaaaagcc atctctgtct ttcccgacgg
    33301 acaagctagt gtcacaagga atcagctaat cagcaaaatc tggcaagaat tgttcactta
    33361 tctttctcac ccagaaaaag cgttggactt ctataaagaa aaatcattcg tattgggaaa
    33421 aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga
    33481 tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg agtttgtctt ctggcgaaat
    33541 cagtctcaca tctatccgta cgcaataatt atcagatttc acaaaaggac acctgatctc
    33601 atacagtgtc cttttttctg aattctcttc aaaagactct ccttattcta tcagctgaca
    33661 gtttctctat cctctagaaa aaggtattct aaaaatatct ataaagcatg gtacactttc
    33721 tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt tacacgatca aaagagatag
    33781 caggccgctt cctttttcaa tcttatagat agaccatggg taacgataaa aacaaaaaag
    33841 aacttgcgac ttattttgcc gtaagttctt ttttagctag tgccttaatc tcttaatccc
    33901 aacaaacgtc tcgcatagat cacctgacca gagtgcatag cagcatcatc aataatagaa
    33961 acgagacgca ttcctcgtgt gactggtggt gtccagttct cgtcaattac ttcatctagc
    34021 tcggaaggtt ccagccttct taagtaagaa atagcaaaat cagcagctcc tcgcaagtat
    34081 cctagcagat cttctatttg attagcttgg atctgctttg cttgttccag actatgcttc
    34141 caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc
    34201 caaattggag aagttcctgc taaatcagca atctggaaat ctaattctcg agcggtatgc
    34261 caagccagcc aagtcattga tttgatctgc ggggctctat cttccactgg aaaacgattg
    34321 gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca
    34381 ctctcgcata gcatatctaa ataatccatt tattttcctc ctttttattt atcctactta
    34441 ctttttccta attctaggat acctacttat atgttactac acacccttat tttttacact
    34501 ttaacagtca ccatcatcaa tagtttggag attgaaagaa aaagatagtg tcgaactatc
    34561 aattgaacaa gaagcaacaa tatgaacgtt tcattaatat tctttaaata aacaatgatt
    34621 tttcaaacga acagtttaag actaggagaa aaagagtata ataggaaaat gtttgaaagg
    34681 aaaggaaggg gaaaaatgaa aaaaagggca agtttactag tcctagttgc tttgttgttc
    34741 aatatctttt cgtatggtgt ggccgtttca gcggaaacat acattcctaa agaccctggg
    34801 aatatttata atgatctaac tggtaactat ggcttgttag gaattgctag tcaattccac
    34861 gtatttgcta aaaataagac aactgtgaat gcacatacag acggaaatat agcaacacat
    34921 gaacttgatg caaaaaataa ttttggtaca gagcttcatg aaggtttatt gaaccaagaa
    34981 ataaattata tacaaaaaac tgattctttc gctaattctt caggcatacc tacaggtagc
    35041 acaagaatga ataaatttgt cgttggcagt aacgtggatg tagcacttca agatggtcat
    35101 cctacagtca atggatccag gcttgatcac ttaactccag atgattttta taaagatcgt
    35161 gctgggcatg aatatattga tttcgagcag gaatttgcta aattgaatgt agcagcaaat
    35221 gatcttgcaa cgatcactcc agctaaaaca tatactgctg cagattttcc agacatgaac
    35281 aatcggacaa tcgatttaac tgatttatca gataccgggt ttttattggt aaatatcgat
    35341 gcagaagtgt taacgatgaa tacgccactt caaatcatta atccaaatga tcaagtagtt
    35401 gtatttaatg tcatcaacag ttcttctgca ttgaacgtac agtcaccaat caaatacaat
    35461 gatcgttcaa atcatgaaac agaagatttt tcggatgcga atatctcttg gaattttgga
    35521 aatgagatga ctgatttgac aatcagtgct ccttttcaag gaacaatcct tgcgcctaat
    35581 gctactatcc gcattactca aaaccaagac ggaacgatta taggaacaaa tgtgatttta
    35641 gatgcagcta ccaatcgttg ggatcctaac gaaatattta tcagcgacaa tggtacagat
    35701 acgacagaca caagcgacag tacagataca tctaccacaa gtgatagctc agattcatca
//
LOCUS       DASOUD010000016        35543 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.016, whole genome shotgun sequence.
ACCESSION   DASOUD010000016 DASOUD010000000
VERSION     DASOUD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.016"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            122..1165
                     /locus_tag="U4A29_000764"
     CDS             122..1165
                     /locus_tag="U4A29_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEO0679907.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1227..1691)
                     /locus_tag="U4A29_000765"
     CDS             complement(1227..1691)
                     /locus_tag="U4A29_000765"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO0679908.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            1936..2883
                     /locus_tag="U4A29_000766"
     CDS             1936..2883
                     /locus_tag="U4A29_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO0679909.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(3094..5166)
                     /locus_tag="U4A29_000767"
     CDS             complement(3094..5166)
                     /locus_tag="U4A29_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEO0679910.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(5099..5476)
                     /locus_tag="U4A29_000768"
     CDS             complement(5099..5476)
                     /locus_tag="U4A29_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO0679911.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(5605..6183)
                     /locus_tag="U4A29_000769"
     CDS             complement(5605..6183)
                     /locus_tag="U4A29_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO0679912.1"
                     /translation="MRGNMKMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYL
                     SLRFLGYFTKESTIDPEDSYFWMINDFIATLP"
     gene            6315..7607
                     /locus_tag="U4A29_000770"
     CDS             6315..7607
                     /locus_tag="U4A29_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO0679913.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            complement(7667..8785)
                     /locus_tag="U4A29_000771"
     CDS             complement(7667..8785)
                     /locus_tag="U4A29_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEO0679914.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8799..10220)
                     /gene="arcD"
                     /locus_tag="U4A29_000772"
     CDS             complement(8799..10220)
                     /gene="arcD"
                     /locus_tag="U4A29_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO0679915.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(10466..11587)
                     /gene="mnmA"
                     /locus_tag="U4A29_000773"
     CDS             complement(10466..11587)
                     /gene="mnmA"
                     /locus_tag="U4A29_000773"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO0679916.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            11991..12590
                     /locus_tag="U4A29_000774"
     CDS             11991..12590
                     /locus_tag="U4A29_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO0679917.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(12740..14013)
                     /gene="efmA"
                     /locus_tag="U4A29_000775"
                     /pseudo
     CDS             complement(12740..14013)
                     /gene="efmA"
                     /locus_tag="U4A29_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(14284..14628)
                     /locus_tag="U4A29_000776"
     CDS             complement(14284..14628)
                     /locus_tag="U4A29_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO0679918.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(14765..15910)
                     /locus_tag="U4A29_000777"
     CDS             complement(14765..15910)
                     /locus_tag="U4A29_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO0679919.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSCQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLEQLVRAVKK"
     gene            complement(16090..17064)
                     /locus_tag="U4A29_000778"
     CDS             complement(16090..17064)
                     /locus_tag="U4A29_000778"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO0679920.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(17144..18019)
                     /locus_tag="U4A29_000779"
     CDS             complement(17144..18019)
                     /locus_tag="U4A29_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEO0679921.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     ALIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKEWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            18149..20056
                     /locus_tag="U4A29_000780"
     CDS             18149..20056
                     /locus_tag="U4A29_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEO0679922.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(20596..23886)
                     /locus_tag="U4A29_000781"
     CDS             complement(20596..23886)
                     /locus_tag="U4A29_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEO0679923.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESCTYT
                     NAQEGFALMVRDAVPKDHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIKLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSFLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNESPNESPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVV
                     YVMKQRKK"
     gene            complement(24158..24703)
                     /locus_tag="U4A29_000782"
     CDS             complement(24158..24703)
                     /locus_tag="U4A29_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO0679924.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(24715..25407)
                     /locus_tag="U4A29_000783"
     CDS             complement(24715..25407)
                     /locus_tag="U4A29_000783"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO0679925.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(25426..25710)
                     /locus_tag="U4A29_000784"
     CDS             complement(25426..25710)
                     /locus_tag="U4A29_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679926.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(25723..26286)
                     /locus_tag="U4A29_000785"
     CDS             complement(25723..26286)
                     /locus_tag="U4A29_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO0679927.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            26513..27163
                     /locus_tag="U4A29_000786"
     CDS             26513..27163
                     /locus_tag="U4A29_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEO0679928.1"
                     /translation="MIKRNWPWLLALVLLFLFGFSFVLGLLLIGVVLVLWGLFGSRRK
                     KGKQEEIPALTNELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAKLKAI
                     DLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIKNKQT
                     YEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQKD"
     gene            27287..28483
                     /locus_tag="U4A29_000787"
     CDS             27287..28483
                     /locus_tag="U4A29_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO0679929.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(28584..29960)
                     /locus_tag="U4A29_000788"
     CDS             complement(28584..29960)
                     /locus_tag="U4A29_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0679930.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIISILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(29997..30863)
                     /locus_tag="U4A29_000789"
     CDS             complement(29997..30863)
                     /locus_tag="U4A29_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEO0679931.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(30952..31977)
                     /locus_tag="U4A29_000790"
     CDS             complement(30952..31977)
                     /locus_tag="U4A29_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO0679932.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(31995..32669)
                     /locus_tag="U4A29_000791"
     CDS             complement(31995..32669)
                     /locus_tag="U4A29_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0679933.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(32853..33746)
                     /locus_tag="U4A29_000792"
     CDS             complement(32853..33746)
                     /locus_tag="U4A29_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO0679934.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNDR"
     gene            complement(33820..35517)
                     /locus_tag="U4A29_000793"
     CDS             complement(33820..35517)
                     /locus_tag="U4A29_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO0679935.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT        10187 a         7576 c         6374 g        11406 t
ORIGIN      
        1 cgaaacaaga ggtcgtgaca agacttttgt cacgacctct tgttcttttg tcaaaatact
       61 ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag gaaccgatca
      121 aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc gcgaaatctt
      181 acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg ctatttatct
      241 gccacagtta ggtggagcga ctattgctat tttattgggt attttattag gaaatacttt
      301 ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac tattagaatt
      361 ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa ttggtataaa
      421 aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt acttgattgg
      481 taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa acgctgtttg
      541 tggttcttct gctattgctt cgatcgcccc ttccatccat gcgaaagagg aagaaaaagg
      601 acagatcatc acactagtca atctactggg tactgtgatg atgttgactc tccctttcct
      661 tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg gtggcacact
      721 ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg ttgtcgaatt
      781 cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct atctgtttga
      841 aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata cgcagaaaaa
      901 acgaaaatta ccacttcctt ggtatgtatt agggtttgtg atcttttgtt tggtcaacag
      961 cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca gctggtttga
     1021 gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca tgaaagaagg
     1081 tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga tggctttact
     1141 atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga ttctcgcatg
     1201 gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa caacaacaat
     1261 tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag ttttccattt
     1321 tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg atttgcctgc
     1381 ccaatcgctc gcacagcttt cggactatca acagcaacag ctacttcttt atacgttcgg
     1441 atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa aggcgtacct
     1501 ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata ttcttcaaac
     1561 tgttcttgtg tgcgcgttaa aatttgctga ttcgctgatt cgctttctga aattggctga
     1621 aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc gataggtgtg
     1681 tgtattttca ttttgtttgc tcctggtcag tagactggaa cttcctcctt tttctctatc
     1741 caaaactact tgccaactaa taaaaattgt tcttaaaagt ttgataaacc gaggaaacat
     1801 ggtaaactaa atacgttagt ttaccaaaga atagctgttc ttttccataa aatcaaattt
     1861 aaaaggtgtt atttaaggtt tattaaattt tgaatgtaaa ccatatataa acatacgatt
     1921 gaaaggatag aaacgatgaa agtatcaatt gttgtcccat gttttaatga agaaaaaact
     1981 gttcctcttt tttttgatga ggttgaaaaa ttcaaaggtg cccatgagtt cgaatatttg
     2041 tttattgacg acggatcaaa agacggtacg ttagcagcaa tcaagaagct agcctcagct
     2101 tatccttatg tccgctttgt ctcattttcc agaaattttg ggaaagaagc ggctctgtac
     2161 gctggattgc aagcttctac aggagattta gtgaccgtga tggatgttga tctgcaagat
     2221 ccgcccgaac tgcttcctga gatgatcaac cggatcacta cttctgatgt cgatagtgtc
     2281 ggcacccggc gttcgactcg tgaaggcgag cctgcgatac gcagtttctt tgcgaaaaaa
     2341 ttctaccagt tgatcaataa aatcagcgat acagaaatgg tcgatggcgc tcgtgatttc
     2401 cgcttgatga ctcgtcaaat ggtggatgcc gtcctagaat taacagaata caaccgattt
     2461 tcaaaaggat tatttagctg ggtcggattt aaaacagaat atatttctta tgaaaaccgc
     2521 gaacgtgtgg ctggcgaaac atcttggtcc ttctggaaat tattcaacta ctcgattgat
     2581 ggtatcgtga acttctcgga tgcaccgtta acgattgctt catttatcgg cgctttgtct
     2641 tgtgtcggtt ctggtattgc aattttgttc attattttgc gggcattgtt gtttggtgat
     2701 cctacatcag gatggccttc catggtctcg atcttcctgt tcattggtgg aatccaattg
     2761 ttatgtcttg gaatcatcgg gaaatatatc gggaaaatct tcttagaaac gaaacagcgt
     2821 cctgtttata tcataagaga aacagaaaaa gtgaagccaa atgaattgaa gcctcaccaa
     2881 taatatacgt caaatgaagt aaacattttt ttaaatgaaa aaggactgtg acaaaaatct
     2941 tgtcacagtc cttttttccg aataaatggt gttccagaag tagcttcttc ggaaataagc
     3001 cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
     3061 gaccacttct atcacaacct ctttcatatc ttattacttc ttcattgctg cttttagtgc
     3121 tgctgctaat gggctttctt ctggttcttc tgcttgtgca tattttttca gcaagcgtcg
     3181 ttcttcatgt ttggtcattt tcttcttctg ctctttttta tcaagcattt tttctgtcgt
     3241 tccatcgtat ttacaacgga aataagcacc gtttttacct tcgatgattt ccattttccg
     3301 gtgacattgc gggcatcgat gattagagac ttttggatct tttcttctgc ggtagttgca
     3361 atctgaattg gtgcagacat agattttgcc gtcacgtgtg tttttctcgc gcagattggc
     3421 cccacattct gggcatttct tttgggtaat cgagaaatct tgatattctt gtttgcttga
     3481 tttgatctca ttcaccaagc gttttgtttc tttttcgatt ccattcaaga aggttcctgc
     3541 ttgtttcttt cctgcagcaa tagcttcaag gtctttttcc cagctttctg tcaaatctgg
     3601 tgttactaaa gagggattta ccaatactaa taattgctta cctttaggag atacttgcag
     3661 tccttgaggg gtacgttcca tcagttcaga tttgattagt ttctcgataa tctctgcacg
     3721 cgtagctggg gttcccagac tgtatttttc catttgacca agcaagcttg cttctgacaa
     3781 tggtttaggc ggtgcagata gttctttttt gatggtgaaa tcgggattga ttctcatttc
     3841 tttttcccat ttgactgttt ccgtctcagt ttcagtttcg cctttccatc ctgcttggac
     3901 gacacgattt tgccgaaaaa tgaatcgttc tttaccaaat tcaaccgtaa cttttgtttg
     3961 gctgacttta tggggttgag caaacagtcc taaaaaacga gagacaatca tttgatagat
     4021 tttttgctct tcattactta atttttcata acgtgctctg ttttcagttg gcaaaagtgc
     4081 atggtgatcg gtcactttac tgtcttggaa tacttttgtc tgttggacga tggctccgtt
     4141 tttcaaatac tgcttgacct caggagcaaa gtcgctgact gcttgcaatc gttctttcat
     4201 cgtacttttc atgtcatttg tcagatattt gctgtctgta cgcggataag taacgatctt
     4261 atgggtctca tacaaacttt gtacaagact taatgttttc tttgcagaat agccatagcg
     4321 ttgattggct tctctttgga tctctgttaa atcataaggc aatggcgcag attccgtttt
     4381 taatttttct tggacttctg tgacagttcc tttgcttgta ctgatcaatt gaacaagttt
     4441 ttgtgcttct tcttgtgttt ttaaagcaaa ttgatttttt tgaagcattt tggcttgttc
     4501 ttttccagct tgcaacgtga tcgtgaaata cgtttgtgga cggaactgct cgattttctt
     4561 ttcttggtct ctcactaatg ctagtgtcgg tgtctgcaca cggcctgctg ataaattatc
     4621 cgcgtacttc acagttaagg ctctggtcac attcaatcct accagccagt ccgcttttgc
     4681 ccgggcaaga gcagattcat acaatgcatc atattctttc gcaggtctta gttgcttgaa
     4741 tccagttttg attgctttat ctgtttgaga agagatccat aaccgcttca ctggtttatt
     4801 gaaatgcaca tactctaaga tccagcgagc aacaagttcc ccttctcttc cggcatctgt
     4861 cgcaatgact gcttctgaga catcttttcg ctgagccagc tgcttgattg cttttaattg
     4921 atgtcctgtt ttaggaagtg gtttgattcc tagtttttta gggatcattg gcaacgtgtc
     4981 catttgccag ctctgccatt ccttattcag atcttccggc attttcaatc ctaataaatg
     5041 accaagggcc caagtaacga tcaccttggg tccttcataa taatttttgt gcttttgatt
     5101 agcaccaagc accttgctta ggtctttcgc aacgcttggt ttttccgcta gaattagttg
     5161 tttcataaaa ttcctttccg ctttctactg tgatgggctg atgatttttc aacaacgcaa
     5221 gtcctttgta ttgctgtaaa tcatcgtcac actcttcaca ccagcgctca tctccgtcat
     5281 tccaaaaggc gctgagaaag ttactttttg cattgatata ttcataattt tgatggattt
     5341 cgttccattg tttttcgtac gcttgtcgtg cttcttcgaa agactcgaat gtttcttctg
     5401 cttcaatatc ttcttcccag tcttcaaaaa accaccaagg ctcattgtcc ccgaacatgg
     5461 taattacttg atacatactc gtgctccttc tttcagcttt tctagactta tcttagtcac
     5521 ttttcaagaa agttgcaaga attttctctt ataaaaggat catttaatga acatcttgaa
     5581 aatgatataa atgaatctga ttgtttaagg caaagtagcg ataaagtcat tgatcatcca
     5641 aaaatagctg tcctctggat cgattgtcga ttcttttgta aagtatccta aaaaacgcaa
     5701 ggaaagatag ccgtggatca tactacataa ttctctgctt cggtggactt tttcttcttt
     5761 cgttaattga aaagcctcaa agatctgcaa aatgatctgg ttcgtttcat ggattccctc
     5821 taataaaact gcttcattgg tgtggggcac actgatcagt aactcataca ctgcttgatt
     5881 tgaaaaagca aaggatcgat aagtttctgc atagatctgc agtgcttcct gaccactttt
     5941 tcccacaaga gcccgacgca gctggtcatt aagttcctgc agaagtttcg ctgttagcgc
     6001 tgttttgaca tcacgaatat ttttgaaatg gttatacagc gagggagcct tgatatccaa
     6061 gcgttttgct aaatgctgaa aggttatttg ctgaacatcc atctcttctg ctagttctcg
     6121 aaagcagtcg atgattttct cttgagacag atttcttttt ttcattttca tgtttcctct
     6181 cataaaataa atccttcact ctttcagttt accacgaaaa agaaagagaa aaaagagact
     6241 ttcattgata aactacatat tgtagtttat aacaaaaagc tgtagatgaa aattttcaga
     6301 aagaaggagt ctaattggct gcaagaataa aggaaaaatt atttaggaaa agagaaactt
     6361 gggaacaaaa cttgtttgtc ttatggtttg gaacatttat ggctggtatc ggatttagtt
     6421 tagtcatgcc ttttatgtca ctttacatag atacgttagg taattatagt acgtcacaat
     6481 tgaacttttg gagcggattg actttttcat ctactttctt ggtgactacg ctgatctctc
     6541 cttggtgggg aaaattagcc caccaaaaag gaagaaaatt gatgctgttg cgtgcttcat
     6601 taggaatggc aatcgtcatt tctttgatgg gttgcgtgac tagtgtgtat caattagtcg
     6661 cattgcgtct gcttcaaggg attttttctg gttatatcag taatgccgct gcccttgtcg
     6721 caactgggac gccaaaagaa aaaagtggtc aagttcttgg aactttagca actggttctg
     6781 ttacaggaac attacttgga ccgcttcttg gcgggatctc tgcttcagcg tttggctatc
     6841 gccctacctt tttcattaca gggatcatct tatttttcgt cttcttgttg agtctgtttt
     6901 tcgtccatga aaaatttgtc ccagtgaaaa aagaaaacat ggtttctgcc aaacagattt
     6961 tcaaagactt gaaatatcca cacgtcgtca tcggtatgtt cgtcacaacg atgatcatcc
     7021 aagcatccaa caattccatt agtccaatca tcagtctgta cattcgccaa ttgctgcatg
     7081 gacatgggaa tgttacgttg atcagcggaa tcatcgcttc gatacctggg attgccactt
     7141 tgatagccgc tccaagattt ggtcgcttag gtgacaagat tgggagtgaa agaatacttg
     7201 cgattggttt gggttttgcg attctggttt atatcccaat ggcattcgtc acgaatgttt
     7261 ggcaattagc tgccttacgt tttttgatgg gtatctctga tgcttgtctg ctccctgctg
     7321 tccaagcatt gatcacaaaa tattctcctc atcaagctgc tggacggatc ttcagttata
     7381 accaatcttt ccaagcaact ggtaacgtag taggacctat gatcggctcc agtgtttcta
     7441 gtgcatttgg ttatcgtggc gtctttcttt cgacttctct tcttgttttg gtcaacttct
     7501 tgcttgtacg acacaataca caagagatcc atcagcaaga aactgcagaa gaaaaagctt
     7561 caggagtctc catatcttca gggcactctc cttctcatag acaataagcg taaatgaaaa
     7621 acactgcttg aacagttgtc acagactgtc cttgcagtgt ttttgtttag gcctgttttt
     7681 tcaagaactc agataagagg aataatccct tttccaaggt ttctttttta caacagtaac
     7741 ctagtcgggc atgcttcggt aaatcaaagg cacttcctgg tactaatagt actcctgttt
     7801 cttctaataa attgatacaa aatgtctggt catcttcttc gatggctaat ttaatgaaag
     7861 aagtagagac gtaattggga gcgacaagtt caactttcgg ttcattggct acccattgtt
     7921 ttaaaatcac aaggttctct gtaatgatct ttttgtttcg ctccaatatt ttttctttat
     7981 tcttcaatgc atgtacagca agatcatctg acaggactcc gccgcaaatc atcgtgtagt
     8041 ctcgatactt tcgaaaaact tctgccaact caggtccagt agctgtccag ccaatacgaa
     8101 tacctggaat ggagtaggtt ttggataggg aattggttgc tatacctttt tcatagacat
     8161 ctacgatcga caagaattca cctttatccg ttaaaggcgc atatacttca tctatcaata
     8221 catatgcatc tactgtacga gcaatttctg cgattttttc taaagttttc cgatctagca
     8281 atgtgcctgt aggattgttt gcactattca aacagatgat ttttgtttca ggtttgacta
     8341 aggcttctag ctgttctaaa tcaaactgcc aatcttcttc ttccttcaaa tgaacaaagt
     8401 caacagcggc acctaaagat ttggggatgt catataattg ctgataagta ggaagcatcg
     8461 aaacaacatg gtctcctgct tccactaacg cgtatagagc taaaagatta gcaccagtcg
     8521 ctccattcgt ttgtaagatg ttttccggat cgacggtttg ataaagagaa gcgacttctt
     8581 gtttgaaaac atctgatcct tcgatccagc catagtccaa tggttcgttt ttgcgttttt
     8641 cgaaaaattc acttacgcta gtcccatcta agccaacgag ttcgtctaat gtcaatgctt
     8701 cgatcgagct ttgtgcaata tcatataccg ctttcttttc ccatttattt agccattctt
     8761 ctacaccgaa atttgcgatt ttcatgtttc tttccccctt atatcttgat ccagcctaca
     8821 gcaagcaacc cgattgaaag cagtgcggcc acacaaatca gcccaatcat tactttttct
     8881 tttcccgtaa tttgatgttt ggtttcacga caagcgatcc agtagaagca aaaaccagga
     8941 agataggcaa tactacataa aaggacataa gaaaatcctg ctaaaatgat gcccatcaat
     9001 tcaaaagcta cagcaatccc accgatcact acttgttttt tgattccttc ttttaaagaa
     9061 aactggactt gatagatccc gacaagtaga taacaaatca aaattgccgc tgtacataaa
     9121 gaataggcaa attgataggc ataatcagta actaagaaag tcaataagaa tagattagtc
     9181 aagattgcag taacaactaa cgaataagtt ggggcatttt ttttattcgt ttttccccaa
     9241 cctttcggca acagaccgtc tcgagccatc aacatcgttg tttctgctgg gagcatcgtc
     9301 caagacaacc aagaaccaat gatcgaaata atcaagccga tattgatcag cgctgctccc
     9361 caatttccca cgattccttt taaaatattt gccatagcag gttgtgaaat agttgctaac
     9421 tcttcttgag ataagacgcc gtatggcaag atcgaagcaa ggatatagat aaacaataaa
     9481 caaagcagac caatgatcga tgcttgctgg gcttctgaac gtttcttcgc gcggttggct
     9541 aaaacacttg cgccttcgat ccctacaaag acccacatca taaccatcaa acaaccttta
     9601 atctgcaacc agatcgttcc ttgctgccct gttccattca ctagattatc agatacgtta
     9661 ccccaaaaat cagcagtgaa tatattcact ttaaatgcga taaatacaaa tattaaaaat
     9721 aggaataatg gcactaattt acagatcgtc acgattgtat tgatgacgct cgctccttca
     9781 actccattgt ttactaataa agtcagaccc caaataaaaa tacttgcaaa taaaatagaa
     9841 ggaacatttt gtccaccttt gaatatcgcg aaaaattctc ccatggcgct catcagcatc
     9901 gttgcaaaag caacatttcc cagccacgca gacaaccagt aaccccaacc actgatgaag
     9961 ccacctaatt taccaaatga agcttccgcg taaccgaaaa taccaccatc tagatctgga
    10021 cgtttttctc ctaaatgatt cagcgataat accaaagcta aaataccaat cccaacgatt
    10081 atccatgaaa ggatcgctgg ccctggagct gcagaactag ccagatcact tgtgatccca
    10141 aacacaccgc ttccgattga cgaactgaca accagagcaa ccagtcccca tttgctaata
    10201 cccgttgatt tcttttccat ttccatcacc ctcacttata aaattgaaca aatcacttgc
    10261 aaaagatttc cttctatttt gcaactgctc ttgcttaaca aaaagcaact ttattcatat
    10321 tattggatat tatgaacttt ttacacaagt atttcaagta taaaatgaat aaaaattaat
    10381 tttattgcaa aaaaacagct atgctatcca aaagaaaagg tgtttctttt gttagccata
    10441 gctgttttga ttcatttaat atcgcttaaa cgtattgtaa aacttttgtt tcttgatatg
    10501 cgtggtcgat caaaccacca cccagacatt ccatttcatc ataaaataca actgcttgtc
    10561 caggagtgat tgcacgtaca ggttcgtcga atacgacttc tgcatgatta tcatccaata
    10621 agcgaactgt cacaggaacg tcttgctgac gataacggaa tttcgctgta cacttgaatt
    10681 cttttggcat tggttcattc gtcgtaaagt ggatttcact tgcatccaag cttgtggcat
    10741 acaatgcagg atgatggaac ccttgcccca catataatgt gtttgtcgct agatcttttc
    10801 ctacaacaaa ccagggttct tgcgaatctc ctccgccacc gatccctagt ccttgacgtt
    10861 gaccaatcgt ataatacatc aagccagcat gttgcccttt gacttcacca tcaagagtga
    10921 ccatatttcc tttttttgct ggcagatagt tgctcaaaaa ttgtttgaag ttcttttcac
    10981 caataaaaca gattcccgtt gaatcttttt tcttagcagt cgcaaggcct gcgcgttcag
    11041 caattgcccg tacttctgat ttttccatgc cgcctagagg gaacatcgtt tttgaaagct
    11101 gttcttgtga taattggcta aggaagtaag tttgatcttt attattgtct actccacgaa
    11161 gcatgtggac cacgccgttt tcatcgcgag tgacttgcgc ataatgtcca gtagctacat
    11221 aatcggcgcc aagatccatt gcataatcca agaatgcttt gaatttgatt tctttgttgc
    11281 acatcacgtc tgggttaggt gtacgtcctg cgcggtattc ggctaggaaa tattcaaata
    11341 cacgatccca atattctttt tcaaaattca cagaataata aggaatcccg atttgagcag
    11401 ctactttcgc tacatcctta taatcttccg tggccgtgca tacaccgttt tcgtccgtgt
    11461 catcccaatt tttcatgaat attccgatca cgtcgtatcc ttgttccttt aacaaaagtg
    11521 ctgttacaga tgagtcgaca ccgccgctca tccctacgac aacgcgtgtt ttgctgttgt
    11581 ctgtcatgat atcaccatca tttcaattag attctgaaac atctacgagt tgaaggtcgc
    11641 aacttttcag tattttacat tatacgtcat atttctgaaa atttcatccc ttaattatca
    11701 agtcgtaaaa atactgttct actggaccta ttcagtacac aaacatgata gtcaaccaaa
    11761 aaatcactca tcttttgagc attcgatata aaaaattggc atactcataa aaagggccag
    11821 cctttcggca ataaggaata attttataaa ttgtgaaaaa ctatttttca aaattgcttc
    11881 ttatcgctta aagctacacg tctcatttac aaccttttct ttttagatgt tatgagttgc
    11941 tcagataagt gattttgttt actctaagaa aaaagggagg caaaagatac atgaattact
    12001 ttaccagtga tatgcacttt ttccatgaac gcttattagg aaaaaacgac tttgctccgc
    12061 ggctctttgc aaatgtggat gaaatgaacc aacagttgat cactagctgg aatgcagtaa
    12121 taaaagaaac cgaccatgtt tatcatctag gcgaccttgc tatgcatccg caatatgaaa
    12181 aagcaaatgc agaaattttg gaactggtca ctcaactgca aggaacaatc cacttgatca
    12241 aagggaacca tgacagtcgc gcttttttcc gttatttaga aaaaaatgat cctaggctta
    12301 ctgacggaag tcccaaattt tatttttatg atgtaggtgc catcgttaaa ttcgaccatc
    12361 aacaatatta cttgactcat tatccgatgt tgctcgggcc taacgacact atccgcaatc
    12421 ttcatggaca tatccatcat tacagcgtac ctatcgagaa cgatgtcaat gtaggcgtag
    12481 acgcaccgga aagggaactc ttaaaggaaa aactaccatt tggcactccg ttatctgagc
    12541 aacaaatcaa agaaatctat gaagcaaaga ggaagtacga taggtcgtga aaaaactgcc
    12601 ctatatcaag tattaaacaa ataaagataa ttactcatat ttttcatttg agtaggctta
    12661 ttgccacagg aacattagga tcagaaatgg agttgtaaca agacttctgt cacaactcca
    12721 ttccatacta ttcgtctttt tactcatggt gttcttttac caattttttc tgtaattctt
    12781 tatcataatt tcgagcagct gacgttaaaa ataaaacgat tccgcataat aaaatcccga
    12841 ttccagaaaa caagaacatt ttttctaccc ctaaacgatc agctaatggt cctgcaaata
    12901 ataatccaat tggacctgcc aggttcatca aagagttgaa gttccctaag acacgaccta
    12961 aaacattcga ttcataactc tgctggatca ttgccatcag cagtgtattg aaatatggcg
    13021 ttgcacaacc tgctcctgca ttcaagataa gaaagtacaa gaacccttga ctatcgtttg
    13081 gaaccaatcc gcttgcaccg atcgtcaggc ccataatgat gtaggctata ataataggtt
    13141 tcattcggtc tttccatgtt cctataaaac tgatcagtgc accgcctaag agcattccga
    13201 ctgcgtagac tacttccact gctcccgcct gtccaacgga accaccaaag taatctagcg
    13261 tcatcaatgg atacagactg attgctggca tgaacaacag catcacaaat gcaccattga
    13321 tcgttatgta ccataaccct ttgttatcca tcaattgctg cagcccaaat tttgcatctt
    13381 taaaaatagt aagcggaact tccaatattt caggagaaac ttttgggatc ttcacaaaaa
    13441 tcaataagcc gacaccaaaa actgccccca atacatctaa taaaataagg taattgacag
    13501 gaacgactga aaacaaagcc gcacctagag ctggcgcaat gatgaaattc gctgattgga
    13561 ccattcctaa ctgtccattc gtccttgtta tgtgagaaga tggaacgatt gtcggcataa
    13621 tcgactgatc gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa
    13681 aactagccaa atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa
    13741 aatcgctaca ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag
    13801 cggttctaca aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact
    13861 caaaattgtc gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac
    13921 catactagtg attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca
    13981 attcgttaaa acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt
    14041 gtatagcgtt tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc
    14101 ctaaataaca atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata
    14161 ttaaaaataa caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc
    14221 ttacaaagtt agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac
    14281 agatcattcg actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa
    14341 tttatcttgg ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt
    14401 tgctgtagtg atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt
    14461 gaatccttct ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc
    14521 gtttgtttct gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg
    14581 atagctaatt ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc
    14641 gataaaatta gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt
    14701 taagttccct aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca
    14761 ctagttattt tttgacagca cgaactaatt gttcaaggaa atactggatg tcgtcttcgg
    14821 tatttcccaa accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat
    14881 acattgcttc taatacgtgt gaaggctcga tattacctgc tgtacaagct gaaccagtag
    14941 aaatagcgac cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta
    15001 agcgaagatt caacacatga gggagtttat tcgttaaatc accatttact tgataaggga
    15061 tttcgtttgc ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac
    15121 gtgcttcacg ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg
    15181 ctaaattttc tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag
    15241 ggatatttac tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat
    15301 gagcagagat acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt
    15361 acgcttgtac cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt
    15421 ctttgattgg catcaaattt ccgatctcat tgttcccata catcacagag accaagatcg
    15481 tatcttctcg taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag
    15541 gcagatatgt aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag
    15601 gatgttcgat agctgtagtg attagatgtc tgccttcttt ttgacacgag aacgctgttt
    15661 ctaagatagc tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt
    15721 ttgcttgcag actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc
    15781 gaccaaatcc atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg
    15841 cttcaattac ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca
    15901 cttcattcat cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac
    15961 caaagaagag ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctatg
    16021 acaaaagtct tgtcgcagct ccttgaagct gctcacttgt gtcacaactt ctttccctct
    16081 tttttttgtt tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt
    16141 ttcatggatg cgtttgaccg cttctcccat taattctgaa catgtaacga ttgttaagtt
    16201 ttctggatga cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg
    16261 tgcattgtct aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata
    16321 tacttctttt gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt
    16381 gttcaagata tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc
    16441 accctcagtt gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc
    16501 tgaaagacta cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc
    16561 tcccgtcaga cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc
    16621 tacaggaata tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg
    16681 agttgcccct gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg
    16741 tttcgccgtg cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt
    16801 cgcacttgct cgcttcattg catcgatcat gatcagtagt tccatatagt agtcattgac
    16861 aggtgcattc gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt
    16921 gatggaaatc tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa
    16981 cactttcgcg attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt
    17041 gtcatcctga tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt
    17101 ttaacatatt tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg
    17161 ttccgttact ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt
    17221 tgtaataacg ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc
    17281 catatgtact gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac
    17341 gttttgtagc tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat
    17401 tcttctttaa aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta
    17461 gtggttggat ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct
    17521 tgcgaatctc atttggaagc atccactcct tacgatagtc ttgataatag gcaaggagaa
    17581 ctgctgccgc gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca
    17641 aaaaacgttc ttctggcaat cccacccagc aatttgcgag ttcctttcct gacctttttt
    17701 cttcgatcaa agcgggaata tcttcttttc tgttgatcca tctagataac tcgattggct
    17761 cttcattgat cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg
    17821 gagaatattc taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa
    17881 ccagagctaa aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt
    17941 ttttaatttt tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg
    18001 atcgcctcct ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt
    18061 cgaattttca attttttatg ttctatttgt taattccttc attttgtaag ataatgggca
    18121 cacgaaacaa aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta
    18181 tgaagcagtc aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac
    18241 aggcggtttc caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga
    18301 tctgcttgcc catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta
    18361 tcaactggca aacgattatg accgaagaaa tgagttgcat gctcaaccac ttcaacctat
    18421 aatcgaaaaa gttgaatcat tgcagtcttt cgacgaattg aatgagcaat ttccagaatg
    18481 gctgttggat acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc
    18541 gcaaacgaac gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta
    18601 tgcatcagac catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca
    18661 gttattagag cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt
    18721 gcaatttgat aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag
    18781 caaaatgtat aatccgcaat cttttgatac atttattcaa tacacagata aactggactt
    18841 gaaaaaactt gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc
    18901 agtgtacttc gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag
    18961 ctggatgatc attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca
    19021 agtcagcgga atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa
    19081 agcagcgtat taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa
    19141 aaaatatttt ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc
    19201 cgtttataaa aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc
    19261 cgtcataaaa ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt
    19321 attcgaccag ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct
    19381 tgttttcagc aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg
    19441 ttcagaatgg gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt
    19501 gatcgtcttt ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc
    19561 tgctaattat ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa
    19621 taacggcgct ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct
    19681 tacacaattt caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc
    19741 tgacggaaaa gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct
    19801 aagctgtgca ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt
    19861 caattgggca acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca
    19921 aatcgatgtc catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga
    19981 attctatgaa acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag
    20041 agtgcgaatc tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat
    20101 tggggaaaat tgcttctcaa atttttgtga catttcggct tatttttcga agaagttact
    20161 tctggaatac catgtatcag aggttgtgaa aaagccgctt agtttcaagt agtaagaaaa
    20221 attttgtgaa aatagctttc cacatttttg cataaatttg acttactact gagaaactgg
    20281 cttttgaaca ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga
    20341 aaaaacggaa aaatagttcc tcatattttg ccgtttgttg agctaatacc gagggtcagc
    20401 tgattgaaca ccgtttagtg gagaggttgt gaaaaagctg tcctgcatca agtaataaga
    20461 acagccaatc aaaagcagca aggagaacct catctataaa aagatagaag gaagtgagac
    20521 atctattggt ctcatttcct tctattttta ctttcctcag tcaattgttt tacatcataa
    20581 aaggagaaat tccacttatt tttttcgctg tttcatgaca taaacaacta caccgattaa
    20641 caaaattcct ccagctaatg ctagcaacgg gttattaaat tctcccgttt ttgggagtcc
    20701 ttgagtgcca tttttttcac tttcttttgc tgtcctgtcc ggatgagaag gttttccatt
    20761 tggtgattca tttggtgatt catttggtga ttcatttcct ggattttctc tttctggttt
    20821 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
    20881 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
    20941 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
    21001 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
    21061 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
    21121 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
    21181 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
    21241 agatggcgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
    21301 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
    21361 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
    21421 gatccaatct tttgatacgg tatgtccaag agtattttct gattgtttct ctcgttcatt
    21481 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
    21541 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
    21601 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
    21661 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
    21721 agacaaacta ttgatcaatt catgagggcc cgttaaaaaa gaaccgccca ttttgaatcc
    21781 attaccgtta ccttctccct caattcccgg tacccagccg tttcgataag caactgagtt
    21841 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
    21901 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
    21961 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
    22021 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
    22081 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
    22141 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
    22201 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
    22261 gttttctgga tttttctctg caatcaactt aatcgggttt tctcttgtcc cgttaacgcc
    22321 tttgggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
    22381 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
    22441 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
    22501 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
    22561 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
    22621 ctgataatca aattcccgat tcgcagtgat agccaagtct tttgcgttct taagcacctc
    22681 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
    22741 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
    22801 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
    22861 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
    22921 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
    22981 aggatcgtaa taatatgcct ctaaaccagt attcgttttt ttcatctcta cgtccagttt
    23041 agtaatcgag ttcgtggccg gaaacagatt cccatgaccg tttccgaata tattataaga
    23101 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
    23161 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
    23221 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
    23281 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
    23341 ctgcccaaac aaatgatctt ttggtactgc gtctcttacc attaaagcaa atccttcttg
    23401 ggcattcgta taggtgcaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
    23461 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
    23521 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
    23581 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
    23641 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
    23701 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
    23761 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
    23821 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
    23881 taacaagtgt attcctcccg caacaaatta gaaaacgttt acttaaagta ttacctaatt
    23941 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaac
    24001 ataacttaag ttgttgataa caaagagatt tattaacgta aaaagaataa gattttttta
    24061 aaataacgta taaagaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta
    24121 ttttgtactt tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg
    24181 tggattcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggcat
    24241 ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac
    24301 agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgtaaacgga
    24361 tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt
    24421 cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg
    24481 ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat
    24541 catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag
    24601 cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg
    24661 caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac
    24721 gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa
    24781 ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa
    24841 aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc
    24901 taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt
    24961 atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac
    25021 catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc
    25081 tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac
    25141 gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat
    25201 gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat
    25261 accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc
    25321 tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc
    25381 cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat
    25441 tgctaaaaaa accacacata acattaggat aacaataata agagatttca tcaaaaaaga
    25501 gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt
    25561 tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc
    25621 tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc
    25681 gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa
    25741 tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat
    25801 cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg
    25861 tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt
    25921 agcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc
    25981 agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc
    26041 gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgcttgac
    26101 aagaagcaga cgatcttgtt cgtcaaaagc aatgatccca acaccgccag gatgaaatac
    26161 cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat
    26221 aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt
    26281 cagcatatct tctcctgctt tctttgtaat cataactgct ttttttcatt tttcaatttt
    26341 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    26401 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    26461 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    26521 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttggtt tttcttttgt
    26581 tctaggactt ttattgatag gtgtagtttt agttttgtgg ggactctttg gttcgcgcag
    26641 gaaaaaggga aaacaagaag aaattcctgc actgactaac gaacttgaaa ctcactatga
    26701 aaaaagcggt atgacagcga atgaaatttc attttttcga caaacaatga atcaaacaaa
    26761 aaatgagatc aatcaattgc aaatgaatat gcagcagaca gccaaactta aagcaatcga
    26821 tctccgccat gatccagtca aagcagcaaa agctttattc aaagaattgg tgaaagaacc
    26881 aaaccgactg catgaagcaa gtcagtttct ttatacgcat ctgccgaatc tagttgattt
    26941 gacaaataag tataatgaga tcaatgacca tgagatcaaa aacaaacaga cctatgaaaa
    27001 actagaagaa agcgcgcaga tcatcgacca gctgtcagct ttaatcgcac aagattatca
    27061 aaaattcgtt gctgaggatc tagatgatat cgacgtagag atatctgttg cgaaacagag
    27121 cttaaagcgt gataataaat atgaaccaga acaaaaggat taactctttt gttcataaaa
    27181 gaagactcgg aacattcaga tgatgtctgg ctgtttcgag tactttttct aaagtagaga
    27241 cattcatgta agaatcgaga acagattcaa caaggaggaa ttttttatgg atcataaacc
    27301 gactgaaaca aaagatgtta caccagttaa cgatacactt gaagatttat tgaacaaccc
    27361 gttttcaact ccaacagata cgctgaccac ttcacaacaa gcggagattg atcagctcca
    27421 agagcaacag acagctgctc gtttaattga caaactaccg gcggaacgcc aagaacaagc
    27481 taaacagcta gcagcaaaaa tcgatgctaa tgatgcacag tctgtcatca gctacggatc
    27541 ggctgcccaa gcaaaattaa gtgaattctc acaatcgatg ttgaatcatg tgcaagcaca
    27601 agatattggt cctgtaggag attccttaac tgaactgatg tatcgtctcc aagaagccaa
    27661 tcctgacgaa ctgcgtgccg gagaagggaa catcttccag cgtgtcttcg ggaaggtcaa
    27721 acaatcgatc tatgaagtga cagcaaaata tcaaaagatt ggtgcacaaa ttgacaagat
    27781 ttcagtgaaa ttagataaag aaaaagacgg tctgctaaaa gataatctga tgttagaaca
    27841 gctttaccag aaaaacaaag actactttga tgcactgaat atttatatcg ctgctggaga
    27901 attgaaaatg gaagaacttc aaacaacgat cattccagaa gcgatgaaaa gagcagaaga
    27961 aactggcgat cagatggatg ttcagattgc caatgattac acgcaattcc tagatcgtct
    28021 ggataaacgt acgcatgatc ttcgcttagc tagacagatc acgatccagc aagcaccaca
    28081 aatccgtttg atccaaaaca caaatcaggc cttggctgaa aagatccaag catctatcgc
    28141 tacagccatt ccgctgtgga aaaaccaagt cgtgatcgca ttgaccttgc ttcgtcaaaa
    28201 agatgcagta actgctcaac gccaagtctc tgaaacaaca aacgacttat tgaagaaaaa
    28261 ttcagaaatg ctgaaaatct cagccatcga aacagcgaaa gaaaatgaac gtggaatcgt
    28321 ggatatcgag acccttcaaa ctactcaaaa tgatttgatc gaaacaatcc aagagacatt
    28381 gcgtatccaa aaagaaggaa aagaaaaacg tcgtcatgca gaagtagagc ttggacacat
    28441 ggaagaagat ctgaaacaaa aactgttgga tttaacccaa tagatcacct ttcgcttgtt
    28501 actaaaacaa acgcccaaca acagtttatt acaactgttg ttgggcgttt ttcttatctt
    28561 tcttctaact tctccattgg agtctatttc tcattcatct tgctgtgtcc atagccatag
    28621 ccaaaataaa tgatcagtcc aataaccatt acgataccga acacgatcca tgtcacagtt
    28681 tgcagacgaa gcatcaatac aatacaagtc aagatggaaa tgattggcaa tagcggaacg
    28741 aatggcactt tgaattctcc agcttttggt ttgccaaaca tttttctcaa ctttagtaag
    28801 cctagactaa caagcatcaa ataggcgagc gccatgatat tcgttagttc cgctaataca
    28861 ttcaatggga aaaatccggc aaagaataca gctgccacac cagcggcata agtcgctttt
    28921 ttcggtgtac gatgcttttg atcgatttct gtcataaact taggaagtaa gccatccttg
    28981 ctgacagcaa acagtaaacg agcaagtgaa tacatcatcg agattgttac cgtcagtagc
    29041 gttaaaatgg cccctacagc gatcaccgcg cccactgttc catgtcctac atacctcatt
    29101 gcaaaagcca ctgggtcatt gactcctagt tcagtatacg gaacgattcc tgtcaagatc
    29161 agcgtcacga tgatgtaaag gacagtagca atcagtatcg aaccaatgat tcctcttggc
    29221 acatcttttt gcgggttttt gacttcctcg gctgccattg caacggcatc aaatcccaag
    29281 aaagcaaaaa acacaagagc tgcaccatct agcacaccgg aaaaaccaaa aggcatgaat
    29341 ggctgccagt tatcaggttt aacgaaaaat attcctacaa tgataaacaa cgcgatgata
    29401 ccaaatttga catataccat ggcattattc aaacgtaatg ctttcttggc ttcaagcgac
    29461 acgatgtaag tgacgaatat cactacgagc accgcaatca agtcaatgta tgtaccattt
    29521 tccggattgt acccgccagt taatgcttgt ggaagttcgg cccctaaact tttcaaaaaa
    29581 ccttgcatat agccagacca tccggaagca acagaggaaa ccgctagtaa aaattccccg
    29641 atcaacaacc accctgtcag ccaggcagca aattcaccaa atacaacata cagataggca
    29701 tatggcccac caatcactgg cactcgcgaa gcaaattctg cgaaacttag tccagacaaa
    29761 atgacaacga ttgcagctaa gacaaatgaa agtgacagtg ctggaccagc attttgattg
    29821 gccgcaaccc ctgtcaccac aaatattccc gtaccgatga tcgcaccaat tccaagcatg
    29881 atcaaatcag acgtttttag ttcttttttc atctcactcg tatgattgag attgatctct
    29941 ttcttacgaa acaaatccat tgaagttcct ccccatttta ggttggaaaa cagcgattaa
    30001 actgttttca tataccctaa aaggatacca cctaaaatga ctaaaaggca accaacaata
    30061 acataaatga gctctttttt cgtttttctt tcgccaagga tgaaaatccc acctaaagta
    30121 gagataatga tccccatttg tgaaagtgag aagccaacag ctaaaccaag tgatttgact
    30181 gttagaagca tgcagatgtt tcctaagccc cataataatc cacagatcat gtttttccac
    30241 acaaactgat cgactttaac tttacggaaa gcaaagaagc ttgcgcctaa gatcattccg
    30301 atactttgag gtaagataat tgacatcgca tccaaattcg cccagttgac gatgatggta
    30361 tatacaccat aaccaatcgt agagaagatc aatgctctaa aacccttacc aaaatcaagc
    30421 attttattat ctgtatctat ttttccttcg tcatcttgtc gggcagtcaa atacgcaccg
    30481 cttacaagaa gagctaaagc aataataccg tatacataat ctttcgtttg tgtccattca
    30541 tggaagaaaa cggctcccgc aatcgtattc aaaattaatt gaaaaccagt agacaatggt
    30601 agccctacag aaacacccat gtacttcatt ccatggaact gtccattttg tccaacactc
    30661 caagctaagc cggataaaaa tccgatcaag aaaatccaag gggtgattac aggactaaca
    30721 acgaaaaaga caactaaaga aaacagaaat gcaccgatcg tcattcctag tgtttgttga
    30781 ttggcatcgc caccaatttt accacttact aaaccaatac taccccatgc caacataggg
    30841 actaatgcta ttaaaatttc cataaccaat ctccttttat ttcacatgat aagaaactta
    30901 tcagcgaaaa cgttaacaac acaagcactt tttttcgttt ctaacgattt tctaacgttt
    30961 ttcattataa aaatttaaga ttgggaaaaa taaagtcatt gttgtccctt ttccttcagt
    31021 tgattcagcc gtcaaatgga tacctaattt ttcggccaga ttttttgcta aataaagtcc
    31081 taatcctgtt gaatgtgttt tgctaagtcg accatttctc ccagtaaatc ccttatcaaa
    31141 gatccgtttc aagtcttccg ctgggatacc gatccctgaa tctttaactg aaaggctgac
    31201 tccttgcgct tccttttcta tgctcacaat gatgtttccg tgatccggcg tatatttgat
    31261 ggcattacta agcaattggt tgaagataaa agcgatccat ttagcatcgg tcaaaacagt
    31321 atgatcctca ccaacgatct cgtagcgaat atttttttgg ataaaataat ttgcctgact
    31381 gcgtatcaca ggctgaatga ttccttttag tccatattct tggatgaggt agtcccgcga
    31441 gaaactgtcg agtcttgcat aatacaaaac ttgttctacg tattcatcga tttttgataa
    31501 ttcattttct agtaatgtat acttttgatc gtcaatatcg aactcgatcg aacgaactag
    31561 caatttagaa gcggaaagtg gcactttgat ttcatggacc catgagtcaa tataatcttt
    31621 ttggtcttgt tgattactga ttgctgattg cattacttct tggtgttcac ggatcagctg
    31681 attgatatat tcttctacca gtttttcttc ttctgtcctc ccaccactca aataattttg
    31741 caaaggggaa gttttttgta tgtcggctaa ttttccccac catcttctct tgctcaagta
    31801 accacccagc agataaaaaa tcagaaagac gccctcaatc aaagctaaat aagcaattgt
    31861 gctcaaatca actttgatgt ttggcgccag ccacatcaca aagatcgtca atgcaataaa
    31921 aaacagccat cccaagatca atgtccactg atctttcaaa tatttaaaaa aattcatctt
    31981 aaccgctcat cctttcaggg aacgatatat ccctgtccta cttttgtttc gatatatcct
    32041 tggatacctg cctgttcgat ttttcggcgt aaccgattga tgttgactgt caatgtattg
    32101 tcatctacaa atcgttcgtc atcccacagt gctcgcagca atttttcccg acttaagatc
    32161 tttccattgt gcttcatcaa aataaataat agacgatatt catttttgct caaatcaatg
    32221 atctctccgt tgatttccat acttccgttg tcaacattca acgtaatgcc attgtggctc
    32281 atcatttcac tagataattc tgtgtagttg taagaacggc gaagtaatgc attgattttt
    32341 gcaatcagta catcgatttg gaaatgtttt gtcacaaaat catctgcccc catgttcata
    32401 gccatgatca tatccatatt cgtattcctg ctggagataa aaatgatcgg tactttcgat
    32461 acttcacgta ttttttgaca ccaataatag ccatccaaaa cgggaagatt aatatctagt
    32521 aatacaagct gtggttcttc tctttcaaag tctgagaaca cttgattgaa atcggtcgtt
    32581 ccaaaggttt caaattgcca tttctcaagt tcctccatga tcaattgtcg aatgatctct
    32641 tcgtcttcga ctaccatgat ttttgccata aaaaatccct ccatctttct tcttgctact
    32701 acaatatcaa agaagccctt gtgaaacaag ttggcttacc tggaaaatgt cattttagca
    32761 aacgttaaaa aaaactggaa atatgcgatt taatcgcgtt tgtctcttcc aagcagctta
    32821 aatttattat gttattctta ataattggtc tattatctgt catttttctt tcgttgtttt
    32881 tgtcttttaa attttcgata acaccaataa ccgaaaagcg ttagcactag caatatacct
    32941 agaaggagta taagtaatcg ataaagatgg tatttttttg tctgttctat ctttgaagag
    33001 acttcttctg taaagggaac tcgtcttcct gtcactagta atcggtgagt gttcacggtg
    33061 taaggtgtac aagtgataag tgtgactaga tcctgctttt ctctaattgc taatgtattt
    33121 gtttttgttg gaaggacaac aattttctca acgacttcat aagcgagcat ccgaccatac
    33181 acttcaagat agaatcggtc atcttgctcc atttcttcaa gatccgtaaa gagttttttc
    33241 tcagcaatac cgctatgggc ggtaatcacg gaatgtgtac tctctcctcc agttggaaaa
    33301 gaggtgcctt gcaggactgt cgctcctcta tctaacaaaa gatcattcgt ttcatcaaat
    33361 aaaggcaagc taacattgat tttcggaata tagatggctc cgatcatatg ttgttcgtaa
    33421 tattcttttc ctggattttg gacatttcta accgcttgct caaaaggatc tttgacttgt
    33481 cccattcctg ggattactga tgtatgagca agcgtctgat ttttcttttc atgcactgct
    33541 aattgttgct catgtttttc ttctttatcg cgttcaagct gcttttgata attctcgatc
    33601 cttctttgat ccaaataatt gctgagagca tctgctaaaa atggatatgt aaaaactgca
    33661 gctccggcaa gaaacatcac tgccataaat aattgtaata aaagactgat ttgggtttga
    33721 ggtctattac ctattttctt tttcattttc ttcaccttct tatttaaaaa aactgcagca
    33781 acctttataa aaccgattgc tgcagcttat ttttgttgct tatacttcag acttcatttt
    33841 agatttacgg taccagctgt acgctccaat catcaaactt attccgatta gtaagaatgc
    33901 caagagcccg ttcccaccag tagcaggaag tgacccgcgt ttcatattcg gtactctttc
    33961 tggccctgga ttttctactc ctacgatctg aatttcttca ttatagcttc cataagagat
    34021 gataaattca gtaaaggctg cctcacctgt tggaagtaca taaccttctg gcgccatggt
    34081 ctctttcatt tgataggttc cataatctaa cccttgaacc tcaaagtatc catttccatc
    34141 tgcgacaaat tctgttgctt gtccgcttgt tgcccactca attctatctg ctcctgattc
    34201 ataaacagct aaaggcgact cccctttata aaatattgcg tattcagaac ggtcttcatt
    34261 ccacaattcg aatcttgcat ctttaagcaa ttcactgctt gatgcatcat atttttcaaa
    34321 ctttcttcca cctgttgtga ctttctctgg cggctctact ccttcttcat aatcaaattc
    34381 atcatttcgt ccgatttcaa taaccgctgt gttgttatgg aaatcatccg cttctagttc
    34441 tgtggaaacc ttcaaattat acgtgatcgt taaatctcct ccacgtgcat ctgctaatct
    34501 acctaaagta tctactttgg ctactccatt ttcttctgtc aaaatcgtca cgacaaaccc
    34561 gttgcttact acttctacta tataatcttg tccttttgtt aaaatcgtct cgccggctcg
    34621 cacttctatt tcctgatcat aataagttag accaactgta ggcgtatctg tgattttgta
    34681 atttttgata acagcgcttc cgttttcaag gacagaatcg atgaaataag gaataggtgc
    34741 tgtgatcgtg taagaagccg tttttcctct ttctagatca atataagaaa tcgtttcttg
    34801 ttctccagct tcattaataa tcgttacttg tctaaggtcg ctttcctcta cattcatctg
    34861 tttatctgct tctctgattt cgtttttcgg atacaaatgc acatctgtat tgtatgtgtc
    34921 tttccatgta gaaccatccc acacgccatc caccatatca ggcatcatca ctggcatcat
    34981 aacaaccatt ggagacgcga ttctggaaat atgagctggc gaataagttt ctaagaaaag
    35041 atagacttta tctctagaag aaggtgcttc ttccgtcact tggacggtag agaataccgc
    35101 ttctccagct tcattcgttg tttgtttgtc aatacttgtc agtcgatatt ttaaaaacca
    35161 actttctgtg tgccgttttt gaatccattc aatcgcttcg gcagaattaa gaccgtcttc
    35221 tcgttccggt gttaaaggat tatctcgaat caaatcgtaa tatacttccg ttacgtcaaa
    35281 caaatcaaaa tcgactcctg gaatattttc ttcaccaaaa tccgtagaaa ccaatccgct
    35341 attcggtcgt tcttccggaa cagatgagaa accttttttg tgtaatgtaa tcgtcacttc
    35401 acttggtttt tgtgcgactt cttcggcttt gactagtttt ccgttcccga aaatccctat
    35461 tcccgcagta acgagtaaca gtacactcat tcccgtgaca atcatttttt tattcatttt
    35521 tttctccccg ttcttttttt att
//
LOCUS       DASOUD010000017        35435 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.017, whole genome shotgun sequence.
ACCESSION   DASOUD010000017 DASOUD010000000
VERSION     DASOUD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.017"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..1282)
                     /locus_tag="U4A29_000794"
     CDS             complement(5..1282)
                     /locus_tag="U4A29_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679936.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="U4A29_000795"
     CDS             complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="U4A29_000795"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEO0679937.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2204..3148)
                     /locus_tag="U4A29_000796"
     CDS             complement(2204..3148)
                     /locus_tag="U4A29_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEO0679938.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3150..3836)
                     /locus_tag="U4A29_000797"
     CDS             complement(3150..3836)
                     /locus_tag="U4A29_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEO0679939.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4310..4630
                     /locus_tag="U4A29_000798"
     CDS             4310..4630
                     /locus_tag="U4A29_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEO0679940.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4784..5689
                     /locus_tag="U4A29_000799"
     CDS             4784..5689
                     /locus_tag="U4A29_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0679941.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            5686..6459
                     /locus_tag="U4A29_000800"
     CDS             5686..6459
                     /locus_tag="U4A29_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO0679942.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(6640..8064)
                     /locus_tag="U4A29_000801"
     CDS             complement(6640..8064)
                     /locus_tag="U4A29_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0679943.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            8275..8721
                     /gene="nrdI"
                     /locus_tag="U4A29_000802"
     CDS             8275..8721
                     /gene="nrdI"
                     /locus_tag="U4A29_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO0679944.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(8786..10768)
                     /locus_tag="U4A29_000803"
     CDS             complement(8786..10768)
                     /locus_tag="U4A29_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO0679945.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="U4A29_000804"
     CDS             complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="U4A29_000804"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEO0679946.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(11681..12742)
                     /locus_tag="U4A29_000805"
     CDS             complement(11681..12742)
                     /locus_tag="U4A29_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEO0679947.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(12953..13519)
                     /locus_tag="U4A29_000806"
     CDS             complement(12953..13519)
                     /locus_tag="U4A29_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO0679948.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(13547..15049)
                     /gene="eat(A)"
                     /locus_tag="U4A29_000807"
     CDS             complement(13547..15049)
                     /gene="eat(A)"
                     /locus_tag="U4A29_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEO0679949.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(15307..16428)
                     /locus_tag="U4A29_000808"
     CDS             complement(15307..16428)
                     /locus_tag="U4A29_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO0679950.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            16554..17495
                     /locus_tag="U4A29_000809"
     CDS             16554..17495
                     /locus_tag="U4A29_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO0679951.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(17645..18541)
                     /locus_tag="U4A29_000810"
     CDS             complement(17645..18541)
                     /locus_tag="U4A29_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEO0679952.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTSFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            18810..20150
                     /locus_tag="U4A29_000811"
     CDS             18810..20150
                     /locus_tag="U4A29_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0679953.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20368..20811
                     /locus_tag="U4A29_000812"
     CDS             20368..20811
                     /locus_tag="U4A29_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEO0679954.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(21076..22494)
                     /gene="nhaC"
                     /locus_tag="U4A29_000813"
     CDS             complement(21076..22494)
                     /gene="nhaC"
                     /locus_tag="U4A29_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEO0679955.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(22897..24120)
                     /locus_tag="U4A29_000814"
     CDS             complement(22897..24120)
                     /locus_tag="U4A29_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEO0679956.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(24151..24990)
                     /locus_tag="U4A29_000815"
     CDS             complement(24151..24990)
                     /locus_tag="U4A29_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO0679957.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(25007..25687)
                     /locus_tag="U4A29_000816"
     CDS             complement(25007..25687)
                     /locus_tag="U4A29_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0679958.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(25689..26762)
                     /locus_tag="U4A29_000817"
     CDS             complement(25689..26762)
                     /locus_tag="U4A29_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0679959.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     misc_binding    complement(26818..27056)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(27098..27331)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(27408..28079)
                     /locus_tag="U4A29_000818"
     CDS             complement(27408..28079)
                     /locus_tag="U4A29_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO0679960.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(28166..30844)
                     /gene="mgtA"
                     /locus_tag="U4A29_000819"
     CDS             complement(28166..30844)
                     /gene="mgtA"
                     /locus_tag="U4A29_000819"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEO0679961.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            31503..31940
                     /gene="ndk"
                     /locus_tag="U4A29_000820"
     CDS             31503..31940
                     /gene="ndk"
                     /locus_tag="U4A29_000820"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO0679962.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(31962..32828)
                     /locus_tag="U4A29_000821"
     CDS             complement(31962..32828)
                     /locus_tag="U4A29_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO0679963.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(32988..35081)
                     /locus_tag="U4A29_000822"
     CDS             complement(32988..35081)
                     /locus_tag="U4A29_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO0679964.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
BASE COUNT        10261 a         7432 c         6240 g        11502 t
ORIGIN      
        1 ttttttatag taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt
       61 ctaacttctg ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc
      121 ctttccattt gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt
      181 gccatttttc ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt
      241 ctttacgcaa tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc
      301 cttctgtaaa accatagcga caatattgct tccaaataaa tagatagatt tttggttgga
      361 tgaatcgttg gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac
      421 gttttgtctt tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca
      481 cttcttcata tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga
      541 attcttctac atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct
      601 gtcgcttttt tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg
      661 cttgataatc cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag
      721 ctaattttat atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac
      781 agtatttttg ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt
      841 cgtctaaggc cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag
      901 agacatgcag gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg
      961 gcgatttaac gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct
     1021 ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg
     1081 cagctaaata atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga
     1141 ctagttcccg ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt
     1201 attgtttaga aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa
     1261 agacggattg ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta
     1321 ctaacataac gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat
     1381 ttccggaaat atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag
     1441 caatgtcttc taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg
     1501 agaatggcac gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa
     1561 gcaaacgata catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg
     1621 gtagaccatg atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag
     1681 cggctggacc gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga
     1741 ttcttgcttc tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg
     1801 cttgtccgac acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt
     1861 gactgctttt ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt
     1921 catctggcat tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat
     1981 caaaacctgt tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg
     2041 ttatctttta tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat
     2101 aacataacag atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta
     2161 gaagaggttg cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca
     2221 tataagcaat ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc
     2281 cgtatttgtc tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg
     2341 gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat
     2401 tcgctccatg actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg
     2461 caaatgcatt ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc
     2521 cgatgttcac atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa
     2581 ctccagcgct tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg
     2641 gccctactgt gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc
     2701 gagaaaagaa catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag
     2761 cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa
     2821 taaaaatttt cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc
     2881 ggctttctcc tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc
     2941 caccgttcac gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc
     3001 agaaaaaggc tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag
     3061 tgtaaatcaa tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata
     3121 aaatatcagg atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc
     3181 gctaatttct tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc
     3241 actggaactt ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt
     3301 gttgattgga ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag
     3361 cagacatttt ccatagctga agccatacct tttagttctt cttcatgctt aggatggact
     3421 tctaaccctt ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat
     3481 ccagcaactc caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa
     3541 atatctacaa tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat
     3601 tctttcagac catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc
     3661 atcccgcgat tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga
     3721 ccgccagtca aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta
     3781 aatgcaaacc cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat
     3841 tttcctccat aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag
     3901 ttgttttctc acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa
     3961 aattatctta cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga
     4021 aataaggtct agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa
     4081 attgcaaagc attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt
     4141 tactaaaaag tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata
     4201 tacaatctgc caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat
     4261 gattttcctc tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat
     4321 ggaattaatc atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga
     4381 tggtttttct aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta
     4441 ctttttgtca aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg
     4501 gattggcgca gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct
     4561 ttccacttgg ttctttgtgg ggatgctact catcagtatc attggcatca agattactag
     4621 cggccactag acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat
     4681 tcaatttttt tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat
     4741 caggtgtatg cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt
     4801 ccaagaatta tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt
     4861 atcccctggc aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat
     4921 gaaagctatt ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga
     4981 gatttctccc tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg
     5041 tatctatcca ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt
     5101 atctgctatc gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc
     5161 gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca
     5221 tccgtctatc gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga
     5281 tgtgcgaaaa atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca
     5341 cttactcagc gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat
     5401 catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc
     5461 tacaacaaat gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga
     5521 tcctgataaa atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct
     5581 attgtctgca caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc
     5641 ttctctattg gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta
     5701 aaacaagaat tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta
     5761 gttgttttgc tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca
     5821 gttgaaatga ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca
     5881 gctgcaagca cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat
     5941 cgaaagtatt ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct
     6001 gtttatttat accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct
     6061 tcgatttcct tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact
     6121 tatactttgg gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct
     6181 tgctttatca atagttccgg tgcatccatc tcagcaggaa tcgtctttta ttttgcttcg
     6241 tcaatcatat ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat
     6301 ccgatcagta tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg
     6361 actcatcttt ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta
     6421 gcattaggat atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct
     6481 gtgaaagaga cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga
     6541 aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac
     6601 gacaggctca caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt
     6661 ggcaaataaa accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc
     6721 taagtggata tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat
     6781 gcctggcaag aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc
     6841 ccatacttga aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg
     6901 ataagaaagt ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt
     6961 accaagtgtg taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac
     7021 attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt
     7081 tccccatgct ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa
     7141 gatcgtccat gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac
     7201 tgctccccat ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat
     7261 cgtttgcagc tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat
     7321 aataatcaag ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc
     7381 tcgtgaacct acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat
     7441 gcaactcaat acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc
     7501 gctagaaaag ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa
     7561 taaaaataat ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc
     7621 gactccccga ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga
     7681 tgccagattt tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa
     7741 tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa
     7801 accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg
     7861 acgtttttta ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag
     7921 tacccaactg aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa
     7981 tacgccacct ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc
     8041 ttttttctgt tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa
     8101 aagtataaca aacctttttt atttttactt aattaataaa aactttaata gaaatataat
     8161 tctaccggct tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca
     8221 ccaaaaaact taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac
     8281 attttatata tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat
     8341 gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat
     8401 gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta
     8461 gacggcggaa atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa
     8521 tatctggaac atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa
     8581 aacttcaata atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct
     8641 ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt
     8701 ttagcagaaa acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc
     8761 tttcgtttct attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt
     8821 ttgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga
     8881 cgatcatgga tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa
     8941 gcgtatcagg tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta
     9001 agtcaactgt gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc
     9061 caatagcgtg tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat
     9121 gtccgttttc tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat
     9181 cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac
     9241 tttcagctga agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag
     9301 tcgctaattc ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga
     9361 agccaccagc ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga
     9421 tcaatgggat caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca
     9481 ttccgatcac tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta
     9541 gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag
     9601 ctcctttgat catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg
     9661 ctgcacccac ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag
     9721 tttcatcaat catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca
     9781 tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc
     9841 agttgatccc accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa
     9901 tttcaatcag accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat
     9961 caggtactac tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga
    10021 cacctaggat cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg
    10081 gtgcttccgg attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg
    10141 gtgttgcacc aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca
    10201 tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg
    10261 ttccggctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga
    10321 caaatgcagg aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt
    10381 tagatggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac
    10441 ggtcttttcc agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc
    10501 ctaatttcac gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc
    10561 cgatgatgac ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta
    10621 attcagagac atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt
    10681 ggacaatgct ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt
    10741 cttccaaagt cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca
    10801 aatccgtttg cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc
    10861 atgacgatag ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg
    10921 taatcagctt ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcgttt
    10981 gttggtttga catcgaccat caagttattg aatactttac ctataccaat catactgata
    11041 gtagaaatca tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct
    11101 gtcaaaaatt ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg
    11161 gcttgtttat tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat
    11221 ccaccgatca catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt
    11281 gtcaaattca gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg
    11341 acagctacag tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct
    11401 acaccgaatg tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg
    11461 gctcccatat aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact
    11521 ggttcgatac ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc
    11581 attagttgaa cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg
    11641 cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta
    11701 taaattgata gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa
    11761 ctttagctac tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct
    11821 cacccatata gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag
    11881 ctgtatttct tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga
    11941 catctcttgc atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt
    12001 catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct
    12061 cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta
    12121 atagatctag agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc
    12181 cttgatatag tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac
    12241 cttgaatttt gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg
    12301 gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca
    12361 attcatcgat atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg
    12421 accagtcggc aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa
    12481 ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg
    12541 aaatatcgac catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc
    12601 gcttttgata tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa
    12661 agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca
    12721 taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg
    12781 actagagtat atatctaaag aaacaaaatt tcaatacttt tattaaaaca attaaatttc
    12841 atttctaaag gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc
    12901 taagcaaaag cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat
    12961 aagaaaccaa ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat
    13021 gaaaataagc attgatcact cttatcaagg cattatggca aactaacaga tagacatttt
    13081 cttcgtcttc gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg
    13141 actccccatt tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt
    13201 tgcttggcag tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca
    13261 gcggcaagct gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg
    13321 aatggatgat tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg
    13381 cctgcatctg tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg
    13441 cgttccaagt tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg
    13501 caaagttccc cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa
    13561 gagaaataat ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca
    13621 tggcaggttt cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat
    13681 aattcaatgg ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt
    13741 ccactttttt ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt
    13801 ccatcccaag ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg
    13861 caaattccgc caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca
    13921 gatgttttgg ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat
    13981 ggataatgga tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct
    14041 cgattgtaaa atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta
    14101 cgcttaaaag tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt
    14161 cgatatcttt taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg
    14221 ctttggatcg tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc
    14281 tagattcagt atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag
    14341 accattcggc tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt
    14401 cattttgagc catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat
    14461 tcccttgata aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat
    14521 cgataaatcc ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag
    14581 ccgctacttg ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga
    14641 aatgagtgtc atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata
    14701 gtgtgctgaa ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt
    14761 cgatttccca tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt
    14821 tatcttttgt ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat
    14881 aaggtagttt gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga
    14941 taagtcctag tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc
    15001 cttggctgtc gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc
    15061 tcgtaaaaaa agaccagtcc tattttcaga actggctaaa gaggatacaa gggttggata
    15121 cacacaaaaa gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct
    15181 tccttgtctc cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa
    15241 tgcacggatc cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt
    15301 tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt
    15361 tttttctaat gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat
    15421 acttaagaca attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca
    15481 aatagcgatt attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt
    15541 tccgtggatc gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc
    15601 gatctttttt tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc
    15661 tcccaacaaa aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc
    15721 taaatccacc tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa
    15781 gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg
    15841 ttgacctgta tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag
    15901 aacgtttctt cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta
    15961 cccttctaaa gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct
    16021 tcctcctaaa gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc
    16081 tcctaaggca taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc
    16141 taaccccaat gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa
    16201 taaatagctg atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt
    16261 tcccctgagc gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac
    16321 attgattttt cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa
    16381 tgaagaagca cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca
    16441 ctttcttctt aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct
    16501 tccatttttc tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa
    16561 tcgcaatagc tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg
    16621 gaaatgaggt cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg
    16681 ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag
    16741 aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc
    16801 aattagatga tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt
    16861 gtttactaaa tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata
    16921 tcttatttgg gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt
    16981 taggtgatgg agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa
    17041 aagttgtaga agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt
    17101 attccatttg gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg
    17161 attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg
    17221 tatctgaatt tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt
    17281 accaacatat tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt
    17341 accaagacct gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt
    17401 ggcgaaaagg acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag
    17461 tccatgcaaa agaaggaata cttggcgcag aataaaaggc tctctgatga gaatcagctg
    17521 aatcagcagc tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc
    17581 tcatgatgcc ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg
    17641 ttttttattt gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat
    17701 cgttagtgat aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata
    17761 ggattggctt aaatgatgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa
    17821 gcaaaaaaat attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca
    17881 ctagtttctt ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct
    17941 cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag
    18001 taagatatcc aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat
    18061 ctctttttat aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg
    18121 caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa
    18181 atgaactatt atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct
    18241 catcgattgc cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt
    18301 gattccaaaa aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct
    18361 ccatattaaa tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag
    18421 ctgatttaat gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga
    18481 tggaaggcaa aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca
    18541 tcttttccgc ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa
    18601 agccagtaaa acgcttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg
    18661 aaataaatca ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct
    18721 gaaaatttgt tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg
    18781 attgtgaatt taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta
    18841 gctccagaca tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag
    18901 gaagtggcga agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggcg
    18961 ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca
    19021 cgcatcacgg attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta
    19081 ctgattgggt ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg
    19141 cttccttttt gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg
    19201 ctgttttaac tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt
    19261 ggctggcttt tgctaaaatc agtgtcatta tcttactaat catttttgct acttatcttg
    19321 tcggtcagca aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg
    19381 gtgggtttac tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt
    19441 cttacggcgg ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg
    19501 cgattcctaa agccatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt
    19561 tgtttttgct tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt
    19621 ttgtgatggt atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtta
    19681 ttgtccttgc tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt
    19741 tcttccgttt gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc
    19801 aagtaccgca acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt
    19861 cttattttgt cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat
    19921 tattgatctg gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat
    19981 tatgggataa aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta
    20041 tcctgttcac gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt
    20101 atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga
    20161 aaattttatt ttatttatgt ggaatagatg gaactattga agaaaggagt tgtgacaaaa
    20221 gtcttgttgc aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt
    20281 caaaaaccct ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa
    20341 ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc
    20401 ggtgtttcaa cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat
    20461 atcttcttca gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc
    20521 atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta
    20581 gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc
    20641 aacgacacag cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctgacaagt
    20701 aaacgagctg atttaaaaga tcctgaagta aaaaaattag cggaagaaat catccaggca
    20761 caagaagaag agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc
    20821 gtccaaataa aaaaagagga tgttccagaa gtgatcagct tcaagaaata agaagaagct
    20881 acttctggaa caccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga
    20941 acagccaaac aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg
    21001 caagcttttg aacaccgttt attcgtaaaa gggattgtga caaaaaaagt cttgtcacaa
    21061 tcccttttta tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt
    21121 cacaaatttt tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga
    21181 aagcaaactg aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc
    21241 tacgaaactt ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc
    21301 tagcgttcta cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc
    21361 attaccaggc agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact
    21421 tgtcatcaat gttgtaaaaa tcaattttgg tgtgttcgtc atctttcttg atactcgttc
    21481 catcacggtt gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat
    21541 aatcaatgac acagtaggca tcatactttc gattcctccg cgactaagca gtgaatcgat
    21601 ttgttggttt ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa
    21661 ggaagtttgt gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat
    21721 cgtcaaaatt gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag
    21781 tgaccagatt gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga
    21841 aagacttgcg gctttacttg tgtgccctaa aatcatgaat aaaaagaaag aaacaacaaa
    21901 tgccggaaca gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat
    21961 agctgctgct agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc
    22021 tgaaacaata gctccaacaa ccagcgctgg atttaggcct agtgttgtcc caataccgaa
    22081 aaatgcaatc ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat
    22141 tgcacacaca acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat
    22201 gaccattaga gttggaatga tccctgcttg tatccataca gcaatcaatg aacctactaa
    22261 aataaaaata aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag
    22321 ccaatctgtc gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa
    22381 gatcgtgata ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat
    22441 aaccattaat acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat
    22501 ctctcctttt ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg
    22561 caattcatcc gctattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga
    22621 atgactccgc ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat
    22681 tcaaatgtat ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca
    22741 ccgactttct gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtatgaagtt
    22801 taccttccat aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat
    22861 tatttttata actttcacac acaagtttat tttttactag aaagtagcac cctcttgttt
    22921 ttttcttgct actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt
    22981 caacagatca agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc
    23041 tttgtcactt ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga
    23101 gattgctacc gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga
    23161 accgagactt gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc
    23221 atgctggaag aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg
    23281 gaacaatcca aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc
    23341 cactttttca tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc
    23401 agcagcgtaa tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat
    23461 attgacatct tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa
    23521 tgcttccaaa gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta
    23581 aaatttgatg tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc
    23641 tggatggacg cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc
    23701 tttagctgat ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc
    23761 accagtttca tcaataaatt gctccactat gctggcggct aatacggagt aagtaccaaa
    23821 caaattatgc ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc
    23881 aattgtgggt ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc
    23941 tacttctttt tctacccaaa acccttcttt ttctaattgt ttaatcaacg ctgctgagga
    24001 ataaaactca tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata
    24061 ttctggtaaa cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat
    24121 aaaaaattcc tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg
    24181 cacgcttgat ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc
    24241 gtctactatt ttttgatacg ttttattttt ttcatcttct tttcttgcaa cgatgatatt
    24301 cacataagga cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt
    24361 aaatccagca tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc
    24421 acgtgctgtt tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat
    24481 atcagaaaca gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg
    24541 caataacaga agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc
    24601 gtctttgata tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac
    24661 agtatttccg atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa
    24721 ttgatgttgg aaagaattca aatcaatcga tccatctgct aaagccgcat ttggttgatt
    24781 gtaatctgta aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac
    24841 gttgtcccat acatctgtat cttctccaat aattcctagt tttacggtag tttctttttt
    24901 gtcagttgta tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt
    24961 aatgactaca aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt
    25021 ttttaatcag ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg
    25081 tagccacaat cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc
    25141 ctaatccgcc tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag
    25201 tcaacgccga gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaataa
    25261 taccgattgg actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta
    25321 gcagtgcgtt ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta
    25381 aagctgcttt cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag
    25441 aaagcaagat aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa
    25501 atagcagacg gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga
    25561 tgattccgat catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg
    25621 tcgcttcgac aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat
    25681 cagtcatttt atcttcctcc ttcgatcact ttgatacgac tatctaattc gtttttttgt
    25741 aaaatattta tttttacacc agatttgatc aaataatcca atgctttttg ccgctgtttg
    25801 gtttctccag ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca
    25861 ttgccatata aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt
    25921 tcgttcgtct gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg
    25981 gcaaaagcag aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga
    26041 ataaaatctt tggtcaattc ttcttcagga ttgctgaaga tctggatgct actacctttt
    26101 tcaatgattc ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc
    26161 atctcatgag tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat
    26221 gcaaggatct gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat
    26281 accttaggtt cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac
    26341 agttgtttag gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc
    26401 actttttgac gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg
    26461 tcaaaaatag ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt
    26521 ctttcttttc gcaattcttt gggagagagc tgactcaaat tcgtctgatt gacaatgact
    26581 tctcctgctg ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca
    26641 cctgagtaac cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct
    26701 actgcctgta tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc
    26761 attaaaattc ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt
    26821 gaggacgctt ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc
    26881 ttcaagtacg atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat
    26941 gctcgggatg aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct
    27001 accgaagctc tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt
    27061 caaaaaaaaa tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta
    27121 ccaccttttt tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg
    27181 ttcgttttaa cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag
    27241 gccattttca gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt
    27301 ccaaacttac tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga
    27361 ggaaaactaa aaagtcaagc acgaaattca aattattttt tctatattta tttttctgtt
    27421 tttttatcta acatttcaat aacagttcga agatcttttc cttcagcaag aagtttcaat
    27481 ttttcctttt cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg
    27541 cctaatcctg ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct
    27601 gaactagctt ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg
    27661 ttctccgaca cttcgtctcc gaatgtaaac gtgtcaaagt aatcagctaa tttaaagtga
    27721 tccaagatat ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga
    27781 cgttctttta agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt
    27841 gtcagctgtt gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg
    27901 ctacctgctg ctttcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg
    27961 tcaacgattt cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca
    28021 cgatacgttt tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc
    28081 tatacattaa tcctttccaa aacaatacac atagtttagc acgactggtc caaaaataac
    28141 agaagcaaag aaccagcctt ctcgtttata agagttcttg gaatcgatgg atatagattt
    28201 tcttcacagc tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt
    28261 aattgccagg aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag
    28321 aaccgacaat aattcctaat gtagtgataa caaacatggc aaatgttacg ttgctttgga
    28381 taaatggtac tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt
    28441 cgacaaacca tcctgcatgg aataatgcga tgaaaatcac ttgctgtgaa ctatccaatg
    28501 tatggaaact tccgccaact acagcaggac aaataacaaa atacatcaac aagtaagtag
    28561 taatatcaaa tatcgaactt gttgggccaa agtaaaccat gaatttccca atactagatg
    28621 cattccattt ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa
    28681 tgcaggaaac atcatagatt agattcaaaa acagaagttg taaaggcagc attggtaaaa
    28741 atggcaggaa catacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg
    28801 tagccttgat atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt
    28861 ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca
    28921 cagctgtatc tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac
    28981 catctcctaa aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt
    29041 tttgctgagg attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat
    29101 gatcactcat ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt
    29161 gacggcacac tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac
    29221 catgttcttt cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag
    29281 taagataacc aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc
    29341 ttggattggt cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta
    29401 cttttgcaag tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat
    29461 caataaattt agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt
    29521 taccagctgg atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt
    29581 catccacttt ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga
    29641 tagccacatc taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc
    29701 gcaaaacacg atcatcttca tttccttcac aatccatatg atattcaaga atgattttat
    29761 cttgtgtcaa tgtccccgtt ttatccgtgc ataacacatc gattgcacca aagttttgga
    29821 tggcattcaa atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca
    29881 aattcgtcgt aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc
    29941 caaaaagaaa agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg
    30001 gagccatcaa tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa
    30061 agcttgattg aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat
    30121 ttccaacaga aacaatgatt ccttccgcac ttccacttat gacattactt cccataaaga
    30181 caagattttc gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt
    30241 ctactggata gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca
    30301 ataaacgcat atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga
    30361 cgatttcttc cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt
    30421 tcacaagtga tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca
    30481 ttgtcccgct gacgatgacc atagtgaaga taatggctac accgaaaaga tctttatctc
    30541 caggagctgc taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta
    30601 atgtaaatgg cgtgatgtat gctttcaata tttctacgat gaatggtgtt tttttgccat
    30661 atgaaatcgc attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc
    30721 catttgctga tgcgtggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg
    30781 catagatttt ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag
    30841 acagctgttc tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg
    30901 aaaggggctg agaacagata gataatagcc ttggcctttc ctctagatcc ttgtttttcg
    30961 aatgactgtc atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc
    31021 aatcattttt cgaatagtta ttttcccaat tagaccacct cctttttatt tttcaccgat
    31081 ggggatttgc ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc
    31141 atattaaaaa aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag
    31201 cagatacctg cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc
    31261 attggcgtct ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac
    31321 aaatcttatt cggttttcct tcgttaatat aacaactaaa aaagaggatg tcaaatcaat
    31381 tttccatgtt tttcatactt tttttaaaat acacacgtta tattcctgtc tcacgcggtt
    31441 ttatttgtct cattttccac ctcccgctat aatagaaata tagtaggaaa cgaggtgctg
    31501 ttatggaaag aacacttgtt attatcaagc cagacggcgt gagacgccat ttagtcggaa
    31561 gcatcattca acgttttgaa gcaaagggac tggccattgc tgaaatgaag tttgaaacta
    31621 tgactcctga attagcaaaa gaacattatc agcacttgac tgagcgcagt ttttttgatg
    31681 aactgatcga ttacatgact tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag
    31741 tgatcgatat cgtccgtaag atggttgggg caacaaaagc tgtcgatgct gttccaggta
    31801 cgatccgagg agactacgcg ctgccgggga ctgaaaacat cattcacgct tctgattccc
    31861 gtgatgctgc ggtaaaagag atcgcccgct ttttcccgcc attcgatata aacaaaccgt
    31921 ctgaaaaagc ggcgaagtga tacaaattct aaactgctgg tctagttgct gggttcagca
    31981 atggccagca gttttttctc taaggacctc cgttgattct ttcctaacat atattcgagc
    32041 actgtgaaga attcttggta gtctttcgtc tttttttccc ctaaagcagc agaaatcaat
    32101 ctttgataat aatctgccat caatgtttca tagatcactg tacttctttc tttatgtgct
    32161 aagctccgtt tcatatcttc attcacacta cgtgcctttt tcaaccgacc attttctata
    32221 taaaacgaag tcaggttcat taaaaaagta aagagatact gctcatgatt gttcaactgt
    32281 gctaatcgtc gcattctttt tttgattttc aaataattgt tctcaatgaa ccgatcactg
    32341 aatatccaag gaaaatcagc tagaatagat agttcaaatt gaccatagtc tggtatctgc
    32401 atcaagtatt cttcaatgat tcggcattca taacgtaaaa attcaaattc tgtctcagat
    32461 acttctgttt tttcgaccat gtgaagactc aaaaccaaat aatacagatc ttctgttttc
    32521 ttaaaatgcc tacggttatt gctgatctct ttataaggat caaagctgtc ttcttgtacc
    32581 cgatctagca ttcgtctttt tttctttaac tttattggtt catccagctg gtttcgcatc
    32641 cattcatatt ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa tgttacaaat
    32701 gacatttttt tcactgaatt tgtctcaatc ccgactagag aagtcttaga acagattcct
    32761 tccgctaatt gggactgtgt caacttcttg ttgactcgaa gtttcttgat caattcacca
    32821 tcatacataa tagttctcct ttttaattta aatttttcca cattcaataa atagatacgt
    32881 taaaaaggaa aaatacacga aaaaaataaa aaaatgtttt tttcataaaa aataccaacc
    32941 ttcaagcatt agaatgttgt ctaactactg aaggttggtt tgattgttta ttgattatct
    33001 ttttgctgtt caacgtctgt tcttgttact ggatataagt tggttgttgt tttattgcca
    33061 ttttttgaaa agacacttgt cgaatgttcg cctttttgct tttcgatggc tatgttacgt
    33121 tctgcttcat ttttatagtc atattttttc ggatcgatcg tttcaaatcc gtttggcaca
    33181 taaaaacgca agagattttc ttgattcagc ttatctgaaa gacttaaagc agatttgact
    33241 ttttcctgat ctttggaaat ttcctcgtcg attcctgctg ctttcgtatc gatgatttct
    33301 cctgtttggt tattgtaagc ttttcctcct aagatggtgt atttgggtgt cacgaagttc
    33361 ccatttcgaa aagccactac ttgatcatgc tgaggagata ataaatccgt accaaaatgg
    33421 agatagtctt tatcatcgat tcccaagaga tgaagtaatg tcggcaaaac atcgatttct
    33481 ccaccgtatt cgtgattgac tgacccttta gtataaccag gtatatgaat cattaatggc
    33541 acacgctgca tttgtgcgtt gtcaaattca ttccatgtat ctgggtcttt tccaagagca
    33601 cttgctaagt ctttattatc tgtatttgag atgccaaaat gatccccata aatcacaaag
    33661 atcgaatttt ggtaaatagc ggattttttc aggtacgtaa aaaattgttc caatgactga
    33721 tccaaataat gtgccgttcg aacatagtta tctactgtgc tatttccagt gttcaaagat
    33781 ggaaaatcga aattcttatc atccgtataa taaggcgtgt gattggttac ggaaaggaat
    33841 ttcgtgtaga acggctgttg tagatgttca agatattggg cagattcact gaataaatct
    33901 ttatctaata ttccataacc tagcgtttca tctgattcat caaaataaga tcgatcaaag
    33961 aaattgtcat aacctaagtt tttatataca tgatctctat tccaaaaact agctacattc
    34021 ccatggaaaa cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg tgcggcttga
    34081 aagacattat ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca
    34141 gtttccaaca tattttcagc atcacttgtc tttccctgtc ctacttcatg aaaaaaatta
    34201 tcaaagctga tcgttgcttg attctggaaa atactattta aaaacggtgt gacttcttgt
    34261 ccgtctacct tcatattaat caaaaactgc tggaaacttt ctaaatggag aacgatcaca
    34321 tttttccctt tagcgatccc aaatgtttcg ggatttggct ctgcataatg tttctttgta
    34381 taatctaaaa cttcatcaat cccattactg cttgcatgcg cgcgaacact gctagtcatt
    34441 cctgttttga ttccatcata aatagtatac gcatcgattc ctagatattt gacaatatat
    34501 gaacggtcaa atgttcgctg cagcaattgt ggacggttgg cttcgctcag cgctagattt
    34561 cctgaaaaaa taagccctga aagacaggtc acagcgatag ccatcggttt gctgacaggt
    34621 tgacttttga taggtatttt cttgatcgtc aaccaaacca gtatcccaat gaacgtagca
    34681 atatccagcc agtagaggac atcatggggt ttcatcaaag aaaaggagct tcctgataac
    34741 ccttgcgaca ctttcgaaaa tcccaataca gatttgactg taatgaaatc tgtgaattcc
    34801 cgataaaaaa taatgttcaa gtacaacaaa agcgtgttta aaatatcaat gatcaaaaga
    34861 atggctaatg ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta
    34921 gttgcaatcg ggttgatcca caagataaag tactggatcg taccttccac acctaacgaa
    34981 aaatctgcat agtaagcaat aatcgtcttc atccagaaaa agaaaacagc tgtcaaaacg
    35041 attgctaaac gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat
    35101 tcacattttt ccttattttg ctcctgaaaa atgaaaattc cctaaaaaag ttgttatctt
    35161 aagatttttt tattctttct aaagattatt ccgtgcactt ttcacagcat aaaaccgctg
    35221 tgatataagc gattgaaaca gtaaggtgat aaaagtcttg tcgcagctcc tgtttagttg
    35281 atgcaggaca gctttttcac aacttcttct ttaaacggtg ttctcctgcc aactcctcgg
    35341 ccacaagtca caatatttat aaaacatgag aagctgtttt ttgaaattgt tccttgtggc
    35401 tcagagttaa acggcaagct caagacctct tgata
//
LOCUS       DASOUD010000018        34658 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.018, whole genome shotgun sequence.
ACCESSION   DASOUD010000018 DASOUD010000000
VERSION     DASOUD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.018"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(311..2203)
                     /locus_tag="U4A29_000823"
     CDS             complement(311..2203)
                     /locus_tag="U4A29_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0679965.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2200..3927)
                     /locus_tag="U4A29_000824"
     CDS             complement(2200..3927)
                     /locus_tag="U4A29_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0679966.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3943..4593)
                     /locus_tag="U4A29_000825"
     CDS             complement(3943..4593)
                     /locus_tag="U4A29_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEO0679967.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4797..5093
                     /locus_tag="U4A29_000826"
     CDS             4797..5093
                     /locus_tag="U4A29_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO0679968.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5285..5905
                     /locus_tag="U4A29_000827"
     CDS             5285..5905
                     /locus_tag="U4A29_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEO0679969.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5997..7163)
                     /locus_tag="U4A29_000828"
     CDS             complement(5997..7163)
                     /locus_tag="U4A29_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO0679970.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7175..7528)
                     /locus_tag="U4A29_000829"
     CDS             complement(7175..7528)
                     /locus_tag="U4A29_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEO0679971.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7656..8678
                     /locus_tag="U4A29_000830"
     CDS             7656..8678
                     /locus_tag="U4A29_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEO0679972.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8697..8858
                     /locus_tag="U4A29_000831"
     CDS             8697..8858
                     /locus_tag="U4A29_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679973.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9117..9551)
                     /locus_tag="U4A29_000832"
     CDS             complement(9117..9551)
                     /locus_tag="U4A29_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679974.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9661..11520)
                     /locus_tag="U4A29_000833"
     CDS             complement(9661..11520)
                     /locus_tag="U4A29_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO0679975.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11638..12797)
                     /locus_tag="U4A29_000834"
                     /pseudo
     CDS             complement(11638..12797)
                     /locus_tag="U4A29_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13104..14528)
                     /gene="araA"
                     /locus_tag="U4A29_000835"
     CDS             complement(13104..14528)
                     /gene="araA"
                     /locus_tag="U4A29_000835"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEO0679976.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14544..15242)
                     /locus_tag="U4A29_000836"
     CDS             complement(14544..15242)
                     /locus_tag="U4A29_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO0679977.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15199..16860)
                     /locus_tag="U4A29_000837"
     CDS             complement(15199..16860)
                     /locus_tag="U4A29_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEO0679978.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17078..18478
                     /locus_tag="U4A29_000838"
     CDS             17078..18478
                     /locus_tag="U4A29_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEO0679979.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18730..19809)
                     /locus_tag="U4A29_000839"
     CDS             complement(18730..19809)
                     /locus_tag="U4A29_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO0679980.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19895..20854)
                     /locus_tag="U4A29_000840"
     CDS             complement(19895..20854)
                     /locus_tag="U4A29_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEO0679981.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20866..22377)
                     /locus_tag="U4A29_000841"
     CDS             complement(20866..22377)
                     /locus_tag="U4A29_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEO0679982.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22399..22962)
                     /locus_tag="U4A29_000842"
     CDS             complement(22399..22962)
                     /locus_tag="U4A29_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO0679983.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22968..23831)
                     /locus_tag="U4A29_000843"
     CDS             complement(22968..23831)
                     /locus_tag="U4A29_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO0679984.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23845..24741)
                     /locus_tag="U4A29_000844"
     CDS             complement(23845..24741)
                     /locus_tag="U4A29_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0679985.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24916..26205)
                     /locus_tag="U4A29_000845"
     CDS             complement(24916..26205)
                     /locus_tag="U4A29_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO0679986.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26177..27694)
                     /locus_tag="U4A29_000846"
     CDS             complement(26177..27694)
                     /locus_tag="U4A29_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEO0679987.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27892..28782
                     /locus_tag="U4A29_000847"
     CDS             27892..28782
                     /locus_tag="U4A29_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEO0679988.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28855..29679)
                     /gene="nadE"
                     /locus_tag="U4A29_000848"
     CDS             complement(28855..29679)
                     /gene="nadE"
                     /locus_tag="U4A29_000848"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO0679989.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29691..31163)
                     /locus_tag="U4A29_000849"
     CDS             complement(29691..31163)
                     /locus_tag="U4A29_000849"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO0679990.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31800..32162)
                     /locus_tag="U4A29_000850"
     CDS             complement(31800..32162)
                     /locus_tag="U4A29_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO0679991.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32385..33137
                     /locus_tag="U4A29_000851"
     CDS             32385..33137
                     /locus_tag="U4A29_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO0679992.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33277..34200)
                     /locus_tag="U4A29_000852"
     CDS             complement(33277..34200)
                     /locus_tag="U4A29_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0679993.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34215..34487)
                     /locus_tag="U4A29_000853"
     CDS             complement(34215..34487)
                     /locus_tag="U4A29_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679994.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10229 a         7211 c         5979 g        11239 t
ORIGIN      
        1 agaggttgtg agcctgccgt ttaactccga gtcacaagga gcaatttcaa aaaacagctt
       61 ctcaggtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa
      121 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agatacaaaa cggaaaaata
      181 gcttctcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacaccgtt
      241 tattcggaaa aagggactgt gacacgactt ttgtcacagc ccctaaaaaa aataataaat
      301 ttgtactatt ctatgaagct tcttcttcag aaaactgact gttataaaga tctgcataga
      361 atccgtcttt ttccatcaat gtatcgtggt ttcctgtttc tacgatcgaa ccatgattca
      421 tcacaataat gttgtctgca tcgcgtattg tagacaagcg gtgagcaaca acgaaacttg
      481 tccggttttc caataggcgg ttcatcgctt tttgaatcag tatttctgtt cgtgtatcta
      541 cactagatgt tgcttcatca aggatcagga catctggatt tgctaagaat gcccgcgcaa
      601 ttgtgattag ctgacgctgc ccttgcgaaa tattgcttgc atcctcgttt aatacagtgt
      661 tatatccttc tggcaattta cgaacaaaat cgtctacgtg agcagcttta gcagcctcaa
      721 tgatttgttc gtctgttgca tcgtcgttcc catatttgat attgtcatag atacttcctg
      781 taaacagcca agtatcttgc agtaccattg aaaactgtgc gcgcaattca tcacgtgaaa
      841 gatcacgggt atctacacca tcgtaacgaa tactacctcc agaaacatcg tagaaccgtt
      901 caagaaggtt gatcagtgtc gtcttccctg caccagtcgg acctacaata gcgaccatct
      961 caccaggttt gacatccaag ttaaaatcag tcattagcaa gttatctggt gtgtagccga
     1021 attgaacatt ttcaaaacga actttgtatg gtgtatcttt ttcttctgga aggccagatt
     1081 tttcattgga catctcttct tcatccaaca cttcaaatac ccgttcggct gaagcaactg
     1141 ttgattggat agtgttcatc aaacttgcaa gttgagtgat tggttgagag aactgtgttg
     1201 tatattgcaa gaacgcttga acatccccta atgtcatgtt cccatttgca acttttactc
     1261 cgcctagtac agcgacgaac acatagccta gatttttaac gaaattcatc aacggcatga
     1321 tgattgccga aataaattga gctttccaac cagcggcata cagattctga ttttcttttt
     1381 caaatacttc ctggtctttt tcagtatgat tgaatgtttt aacaatggta tgaccaccat
     1441 atgtttcttc tacttggtta ttcaataaac ctaaactttt ttgctgatct gcgaaaaaca
     1501 cttgtgattt cggcgctacg atcatcacga tgatcaagct taaagggatc atcaataacg
     1561 cgactaacgt caattgccag ctgattgaaa gcatcatcac gatgacccca atcaatgtga
     1621 ctgcactagt tacaaattga gtcaaatttt gctgcagcgt gcttgcgatg ttatccatat
     1681 cattgattgc tcgagacatg atatccccat ttgtatgtgt atcataatag gaaatcggta
     1741 cacgattcat tttggcttcc aaatcatgac gtaattcata aactgttcgt tgggaaacac
     1801 gtgtcataat aaattgctgt aaaaagttga atacagcaga aatcaaatac atcacgatta
     1861 ctgtcgcaat gatctgcgcg attttatcaa agtctatcgg aaattttgtt acttgttggc
     1921 cttgttgcat ctgttgagcg ccagccatta cacctttgaa tatttctgtg gttgctttcc
     1981 ccaagacctt tggtgtttgg atttggaaga tcacggctgc tattgctagt acaaatacag
     2041 cgatgatcgc aaccatacgt ttagacatat agccaaacaa tcggcgtacc gttccccaga
     2101 aatttttagg cttttcacct tttacaccga gatttctgcc aggtccacca tgtcctggac
     2161 cgcctgctgg tccatgagag ggacgtgtgt ttgttttact catgctaagt cctcctctct
     2221 tagttgtgaa tgaacgattt cttgataagt ttcgtttgtt gctaatagtt cttcatgtgt
     2281 tcctttgcca accagttttc cttcatctag cacaaggatc atatctgcat ccattactgt
     2341 acttactcgt tgagcaacta aaacggtgat actttctttc atattcgttt tcaatgcttg
     2401 gcgaagattg gcatccgttt tgaaatccaa cgctgagaag gaatcatcaa acacatagat
     2461 atctgctgtt ttgactaatg cccgtgcaat tgccagacgc tgtctttgtc caccagagaa
     2521 gtttcctcct ccttgttcca catggctatc taagccatct tccatctctg aaacaaaatc
     2581 tttagcttgt gcgatttcta atgctttcca aatcatttca tctgttgcat ccggcttacc
     2641 atactgcata ttgtctcgga tcgttccaga gaacagaact gctttttgag gaacaaagcc
     2701 cattaattct cgcaaattat attgagtcat gtcactaatg tccgttccat ttatttggat
     2761 cgaaccagat tcgatgtcat ataatcgagg gatcaaattg actaaggtcg ttttacctga
     2821 accagtcccc cctatgattg cgacgatctc gcccgacttg gcttggaaat caatatcttc
     2881 caatgcaagt ttttctgctc catgatatcg ataattgaca tgattgaagg ccaatgttgc
     2941 gttttttcca gcaaaagaaa ctgttttagg attttcaggg tcatggatac cgattttttc
     3001 atccaacact tcattaatac ggtcagctga agcttgtgca cgaggcacca tgataaaaat
     3061 agcggaaagc atcataaagc tgatcaagat ctgcatcgca taagtcataa atgcaatcag
     3121 attaccgact tccaaagtca tatctgctat gtagtgtcca ccaaaccaag tgattgctac
     3181 gtttgtaccg ctcatgatca atgtcatcat tggcatcatc aatgcgacga tcgtattaac
     3241 tttgattgcc gtatttgtat aatctttatt cgctaaatca aaacgatttt cttcaaagcg
     3301 tgtcttatcg aatgctcgaa tcactctgac ccctgtcaat ccttctcgaa ataccaggtt
     3361 taaacggtca gttttctttt gcatactttt gaataatgga acagcaaagt acatgattcc
     3421 gccgatcaaa ataatcatta ctgggatgac aactaagaag atttttgtca actgatgatc
     3481 tttttgataa gcaagtatgc tcgcgccaat caacgtgatt ggtgcattga tcatcatacg
     3541 taaaaacatt tgtgtaacca tttggatttg agtcacatca ttggtcgttc ttgtgatcaa
     3601 agaggccgtt ccatatttat caaacgcatc ttttgtcaga ctctctgatt ttttatagac
     3661 atccgtacgc aattgcttac ccagtttctg tgattcacga gtagcaaaaa atgtatttgc
     3721 gatagcagct aagatactga tcaaagaaaa gccgatcatc acaaaacctg tatgccaaat
     3781 ataatcgaca tctccttgcg ctaccccttt atcaataata ttggaggtaa gtgtcggcaa
     3841 atataaatct gccagtactt ggatcaccat gaaaactgca gctgcaattg ctgaagtcaa
     3901 ggagatccgt tttacgattt ttagcatcta gttcccttct ttctatttat atactccgtt
     3961 tttcagccaa gaaagtttca aattgaattc actgattgct tgctctactg agtaatcttc
     4021 agatgttgaa aataagcgat acgaatgcgc aattgttgta aatacagtat tcatcactaa
     4081 ttggatcagt aattccaatt catgatcatt ttttactttc agcttcgtcc gatcgatcaa
     4141 ttcaagcaac tcttttgcat cttgtttgtg ttctttatgt ggatcgtgag gcgttttttg
     4201 gcgactcgta tgaggagcga cttttcttga ggcacggtaa tccatgttca taaacaagtt
     4261 acgataaaaa gaagcattct cgccttccaa aatctcataa atcattttag aaaaatagga
     4321 ttcgaatcct ttgaatagat caccatcgga ttctttcatt agttgtaaca tatctttaga
     4381 gctgtcacgt ctcattgtct caaagtaata aaaataaaga tcctctttat cttcaaaata
     4441 ctgatagaaa ctgcctctag ggatatccgc taatttgacg atattagcaa tcgaagcttc
     4501 ttttaagggt actctagaaa actcgattct cgcagcctcg attagtcttt tttgtttttc
     4561 tttaggcaaa tggaaaaatg tctgtgtcgg catatggatt ctcctttctt tcaagatgac
     4621 acattgtcat atgacgctat gtcattataa cacctcctac ctgttttgca agacatttgc
     4681 atagtttttt tcattatcat ccgatacatt aaaaaagtga taagaggaat ccttccattt
     4741 gaattaatga gagttgccca ttaaaatagc cacatactaa acaaaggatg agataaatgg
     4801 cccaatcaat tagtgtccaa gacttcaata aattgcctct cgatgctgac agcatcgttt
     4861 tagatacaag aaatacctta gattacacaa aaaaccactt aagccactcg ctttctatcc
     4921 ccgctgctat cttgccaaga caattaaaaa atctagatcc aaagaaactt tattatattc
     4981 taagctatac aggcaggcga tcagaagcat tagccgatca acttactgct aatggttttc
     5041 gtgctgttta tatcattggt gggatggaag ctcttcagtc gagtgttgct tgaatattgc
     5101 ttgttagatt caaaagaggc tgtgacaaaa aatcgtgtca cagcctcttt tataccctgt
     5161 ctttttttag ttatttattc ttcaacctta tcaatatgac ttgatagcct tttctttttc
     5221 gagtaaactg attttgttag tcatagttat aacaatcata gcaataaaag gagaaggtaa
     5281 gcatatggca tctgttttag tagtcaaagg tcatccccta actgcagaag aatcgcgcac
     5341 agttaaggca ttgacgtctt ttttaacaag ttataaagaa aatcatccag atgatgaagt
     5401 gactgttttg gaactttatc gcgatgatat tccggaaatc gatgaagaat tattatcagg
     5461 ttgggaagca ttacgtgctg gtgcagaatt cacttcctta tcagaaagtc aacaacaaaa
     5521 aattgctcgt ttcaatgaat tgactgatca gttccttgct gctgataaaa tcgttatcgc
     5581 aaacgcctta tggaatctaa atatcccaac aaaattaaaa gcttggttcg ataccgtcaa
     5641 tgtagctgga aaaacgttcc gctatacaga aaacgggcca gaaggattag tgacaggtaa
     5701 aaaagcactg cacatccaat ctaatggcgg tgtatacaac ggacaagact ttgcctctca
     5761 atatgtaaaa ggtattttaa actttgtagg catcgatcaa gtagatcagc tattcatcga
     5821 aggaatcgat tatgaccctg accgtgcaga tgagttgatg caaaatgcat tagataaagc
     5881 tgctgcttta ggtaaatcat tttaaaaatt gagataaatg catacaaaag agacagagga
     5941 aaaaatcttc aaagcagcat ttgtgccttg gatattttcc catctgtctc tttttgttat
     6001 tctttctgct ttttcaacgg attttcttca taaagagaag agattgttgt gtaccattca
     6061 aaaacatgag taatcactac tttagctgct gcataaacag gaatccccaa gatcacaccg
     6121 atgattccga acaatttccc agaagtcagc agaactaata aaatcgttac tggatggatt
     6181 gctaactgat tccctagtac taatggagag atcaagcgtc cttctattgt ctgctctact
     6241 gcaaaaacga tcagtacttt gatgatcatg aatggacctg ctacaatggc taaaaagata
     6301 gccggaatca tcgctaaaaa tgatcctaag taagggatca ggtttaaaaa acccgcaata
     6361 atccccaacg tgacagcata ctctaaacca atgaccgcaa aaccaatcat aaacatgatt
     6421 gccacagcaa aagccacagt tagttgtcct cttatataag aagaaacttg actattcatt
     6481 tcactcagca cattcaacgt cggtttttgc atgcgtgtcg gtaaaaattt gacaaaataa
     6541 ggtgctaatt gtttaccgtc tttcaataga taaaataaga taaacggcat cgtcagcaga
     6601 gccactaaaa tagtcgctac cgcgccgaca aaacttccaa gactctgaac tgtactctta
     6661 gatatatctt ggatcatatc gcctgccgaa ttcatgaact tatcacctgc tgtttccaac
     6721 tgttcgcgaa attggttgaa cagcgggtcc cgcaaaatct cggttaattt attatcaatc
     6781 gtatctacat attgagggaa gttactgatg aagctcactg tttgttcctg aatcttaggg
     6841 atgatcacta cgctccccca tgcgattagt gcaacaacca aaacaaacag cccaatgata
     6901 ctgtaaagcc ttggtacttt tcgcttttca aagtaatcaa ctaccggatt catcaagtaa
     6961 tacaagattc ctgctaaaat gataggcaga cctacgattg ctaaaaattg ccagattggt
     7021 gcaaacaaat atgaaacttt tgtaaataaa aagacgatca gcaatattaa caatacaaca
     7081 agtaaggctg tgactacttg attatttaaa aaccagcgcc aaaaccacga taccggtctg
     7141 ttcttcttat tttttccttc cataattcct cctgttagtt ataaaaaatt tggcttccca
     7201 aaccaatagc aggcagtcta tgcggatgaa gatagattcc atttgcaatt tgtccttctt
     7261 ctggacaagc atcaaaaatc aatgatacgt gagctactgt ctttaaattc ccattggcca
     7321 tcggaccgac ataatcgatc gtatatacct gatcatcaaa agcaattgtt ccgcctgctt
     7381 tcaaagaaaa aggttcgtct tttgatactt cttgaatcac cgaatatttt cgtaaggcag
     7441 ctgttgctgt tttatcgaac aaaatcagca tgggttcttc cttgtttatt gcttctgtcc
     7501 caatttctgt aatctttgcc tgaatcattg cactctctcc tttaattgct ttcaaaaaca
     7561 gtataacaca ttctcattct ttagccgtgt ggaataatta tgtgatagaa taaaaacgaa
     7621 aactatttca ttacaattag gaggaatcag ctcatatgat cgaaaagata attttaggaa
     7681 cctatacgcg tcgcgaaagt aaaggaatct atacgatcga ccttgataca gaaaaagaag
     7741 aattagccaa cctttcatta gctgttgaag aaaatagccc tacctatgta gcaaaaagta
     7801 aagctggcaa tctttacaca gttactagtg tggatggatt aggtggtgcc ggtgcgtacg
     7861 ataaggattt tcattttttg aatgcggtaa cagagtctgg cgctcctttg tgttatgtag
     7921 cggttgatga agcacgtcaa ttactatacg gggcgaacta tcataaaggt gaagtaaatg
     7981 tctaccatat attaggagat ggcgaactga aagcagcaga ttcaatattc catcaagaag
     8041 cgacaggtcc tcataaaaac caagatcatg cgcatgtcca ctatacggat ctgactcctg
     8101 accaacgatt agtcgtatgt gatcttggta cagatcgtgt gtatacttat gatgtgtccg
     8161 aaaacggcaa attgaatctt gtaactacct tcactgcaga gccaggaaca ggccctcgcc
     8221 atcttgtctt tcatccaaat ggacaattcg cttatttatt tggtgaatta gacagcacag
     8281 tccgcgtgct ttcttataat aaagaaacag gtgcttttga agaattacag aaaatatcga
     8341 ccttgccaga agaattcgac ggtgaaaatg gaggggcagc tatccgcatt tcaaatgatg
     8401 gcaagttcct ttatgcttct aaccgaggac ataactcgat tgctgtattc gcagtttttg
     8461 aagatggttc ccacattcaa aacaagcaaa tcatctcaac agaaggggat tttccccgcg
     8521 atttcgcact agatccaagc aataattttg tcgtgtgtgc caatcagaac accgataatc
     8581 tgacattata tcgccgtcat cctgaaacag gattactgga attactgaaa aaagatgtct
     8641 atgtaccaga atgtgtctgt gtttattttg atcgttagta agaaaaggag aatgaaatga
     8701 atcagcaaga cgtattagac agactacggg aagaactaaa tattcctttc tttgatggga
     8761 aaatcgaaga taaagagtat agcgaagagg aatatcagaa actcaaagcc gatttacaga
     8821 actactttga acaatatgta cagaatgtag aaaactaatc agctttccat actatctgaa
     8881 aagaacatca ggacaagact tttccgtcta tcctgatgtt cttttctaca aataaaaaac
     8941 cgccgagatg gttagactcg acggaaaagg agtatttact cgattttgag taagtacaag
     9001 gttgttgttg gttataggta tgtcattatc ataaaggatt ttgatcactc tatcataaag
     9061 gtaatattac atttatatta gataactcta gtttttattg ttatcaaaga tttcttttat
     9121 tttttcaaca acaaattagt cattttttgg atggcagctg tcaattggtc tgtcgcttgg
     9181 tcttgttttg cttcagatgt attgaataga tcttcatcta cccctgattc aagatcgata
     9241 ctttggatca tttcttcaca gcctgctaca tatgaaagat aagctttttc aaaattttta
     9301 tgaatgccca ttgcttttgc tggtggacgc aaagtactga ctttcataag cattgcccga
     9361 tatttttttg ttccttcttc aaataacagg atgatctctt caagagtcga tttgtccagt
     9421 tcacttgttt ttccttcatc aattgcgttt ctgatcactt catatttcgg atgcattcct
     9481 tctccgattg cttctgtttc cttgacgatt tgatcgatgg actgcacata atagccaata
     9541 ttcggtaaca tgttcgtttt cctactttct ccattgttgt tattatttat tctacttgtt
     9601 tttatcctca aatgacaagt acttgaaaaa atttaatggt ttctatacag ctcgccattc
     9661 ttattcgtgc tcaaagaaac catctgctaa actatattgt ttccctgcga caatgtcata
     9721 ttggatcaat tgatattcgt cgtaaatctc ttgttgcgag cgattgaatt gcgcttcttt
     9781 ataccactgt ccgttttgat agtacagttc tcgttcaaac gccggaagtt cctgttctaa
     9841 tgataaaagc agctgataaa agccatttgt tgtctgattc gtctgttcca ttagattcgc
     9901 tgcaaaataa aacggactgg tgattgcatc gtgagtcgtt tgcttttcta attctccttt
     9961 actgtcgaac ataagaaatt gtgtttcatg caatgtttgt ttgtcatttt ttgcttggat
    10021 cgtatctgaa tagatccctg gaagatgatc cccccaaaaa acaactaaag tccttctaga
    10081 aagattcttc aattcttccg tgaactgctt cagtgccgtg ctgctatagg aaatatcttg
    10141 cagataattc tctaagtcaa gtgtcccact tccatctgaa gtttttgcag gatagtctag
    10201 attatcatac tttccatcgt agggcatatg tgtctgcatc gtgactaaat gaatgaactg
    10261 aggtgttttt tcttccttga gaagtgtcat tacttctttg taagctgact catcagaaat
    10321 atatggatta ttctctatcg tatccgtata ggtcatcgta cgctcgctga tgaactggtc
    10381 aaatcctaat gtctgataaa catcctctct tttatacatc gaagtattgt atggatggat
    10441 cgctgtcgtc tggtatgatt gtgcgtttaa cgctgaaacg atagaaggaa gctgatccat
    10501 ttttggtaca agcatcgtgt aaggggtggt gagttgcgca ttaaataacg ccatagaaaa
    10561 accagtcaat gcttcaaatt cgatatttgc tgttccaccg ccatagtttt gagaaagcat
    10621 attgccacta tatgtttgat cagctacttc ataataatct gccaatggct caccagatac
    10681 ttctacgccg tttaatcgac taggatctga gaaactttca ctcatcacga acacgatgtt
    10741 cggcgactct tcctctacag ttttattttt ttcatcggct agcttctggt atttttcagt
    10801 gatttctttg atcttttctt tcgaatatcc ttcaggttca tccattggtt ctaccttcag
    10861 attgtacaaa aacccaccga taaaaccagt gttatagtag ttcattttct ggctgtaagg
    10921 tatccatagt gccgttttat tatatgcttt tcgcaacaaa ttatcgggat tgttgaaatt
    10981 actgatatac cctagcagtc caattgccac aagcagtgtc gttaatcgga tcaattggaa
    11041 tcgccttcct tttttcaggc ttcgaaacat gtaccagcaa aacaatccta tcacggttcc
    11101 tgcggcagct aagatcactg taaacagcat agtgccggtc atatctttca gcaatccgat
    11161 ttcagtgatc atcttcaaat cgtctggata aatcggttct tgccgataac tcattttcat
    11221 ataatttgca aaacctaata caccgatggc tacgacatac gtaagggaac ctaaagggac
    11281 agatcctgct agtgcaatca gaaaaataag aataatcagc aagacgagac aggctaagaa
    11341 gaatttttcc gtatgccaag aaaaagcaaa tttcaacgca aggtctacgg atagatcatt
    11401 ttgacaccac tgcaaaaata aattactaaa aacaacaaga aaaaagatac tgatgattgc
    11461 tgctgttcct tttaaaatct ttttctgttt ggtcgaaagt ctgcttatat atcttttcaa
    11521 atgcttgctc cttcatttct atcaataaaa gaatgttgct tctcttattt tagttgtacg
    11581 aaattcgaaa gtaaagaacg gaaagaagaa ttaatgaaat ccaaatattc tctttcttta
    11641 tggcctttct ggtttcttta ataagcggac ctttcgtact ccagaaattt tttctagctg
    11701 ttgtttcact tgattgagct gttcaaaatc agtactgtct atgtcaatca atatgtagac
    11761 atgctcattc ttatggttac gcgatatatc tgcgatcgct agctcgttct cattcagcag
    11821 agaaaatatc tctgctaata tattggtttt cttttgataa aatatagtga atcgaaacgg
    11881 cgcttggaaa aacattcgtg cggatggata attgaccgct tctctaaccg taccgaacaa
    11941 aagataatct tttaaggctt caactgccaa acgtccgctg tctgccaaag cttcctgagt
    12001 cgatccccta gatgaggcaa cataagtata cggtcatttt ctaagaactc ttcttctgga
    12061 aaatcagtca agtatttagc cagatgttgt ttttccaatg cttgcataac agcatattta
    12121 tcaacaattt cacttcttcc tacattgata agtacagcat cttttttcat tttttctata
    12181 ttcgcctgat cgatcaatcc ttttgtatct tctgtcaacg gcagcatgac tacgatgtaa
    12241 tccgaggtag aaagaactgt ttctaaatct gcttgccgaa cataatccaa ctgggcatcg
    12301 cggatagaat aacccaagac atccatccct aagctatagc aacttagcgc tacttttgtg
    12361 cctatcgctc caagaccaag aagaccgatg gttttgcctt gcaattctcg tcccacataa
    12421 gcacttcttt tgttttctgc ctgttctaaa atattaggac ctgttagtgt ttgtaccata
    12481 cgacttgctt cgattatcgg acgggaagat aagagcaaac agcacaaaac aagctctttt
    12541 actgcattag catttacacc tggtgtattc atgacgatcg tcccgttttc agaagctttt
    12601 tcaacgttga tcgtgttcac cccaacacca gctcttgaaa ttgctagcaa atctggtgta
    12661 agccattcat ctttcacttt cgtactacga ataactattc cttcggctgg caaatcatct
    12721 attattttga aatattcccc ttgtaacttt tctatctctt gttctggaat agcatcaatc
    12781 aaatgaacgt gatacataaa accccctcct tactaacaag tatctcgaaa tttctgcaat
    12841 tagacaactg tttaccattt gacttcctag aagcaaaaag agcagttaga gcagagtagg
    12901 ctgagtgata aaaataaggc tatgacaagt tactgccata agccttattt tcgagagcaa
    12961 aatattgagg gtgagatatc ttgataaatg gtgttccgtt agctgacatt gataataaga
    13021 ctaaatttca aaaaagttga aaagcaaatt tcgtcaatct cttcttattt cttaaaacta
    13081 agtggctttt tcacaacctc tttttatcta ataaccactg tttccaactc aactaatttt
    13141 gcccacgttt caatttgatc tgttgttaga tttagcgaga caacggtatg atgaccgcca
    13201 cctgcctgaa tccatgaatg gacaccttca tggaaattcg gtttagtttt ccataacaca
    13261 cgtgcaacag gtaaattagg tgcttcagtc gttggttcaa aggcttctac ttcattgatc
    13321 agcagtttat aatgcgtacc gaaatctgcc atcgatacga ctaccccttc tcctgctttc
    13381 ccatcaaata caagacgcgc cggatcttct ctatctccca ttgataaagg agaaacaacg
    13441 attcttggtt tcgtcgcagc aagcgtcgga tctacttcca tcatatggga ttccaagatt
    13501 gcttcttggc cactagctag ctcgtaagta taatcttcca tgaatcctgt atctttccct
    13561 cgagccatga ttttcagcaa ccgatcaatc gctgcagttt tccagtcgcc ttccccagca
    13621 aaaccgtatc cttctgccat caaacgttgt acagctagtc caggtagttg tttcattcca
    13681 tacaggtctt caaaattagt cgtaaaagcg ttatagcctc cttcttctaa gaaacggcga
    13741 ataccgattt cctgctgagc ttgtactttt acatgttcct cccatgtctt ttcttcataa
    13801 tcaccatagt cgaaggtata caattccttg tactcttcaa ataattgatc gacttcttcg
    13861 tctgatacgc gatcgattac ttgtacaaga tctccgatac caaaataatc aacagtccaa
    13921 ccaaattgaa tctgtgcttc cactttgtct ccttctgtga cacccacgtt tctcatgttg
    13981 tcaccaaaac gagccacttt tattccaaaa ctttcattat aggctactgc aacatccatc
    14041 caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac gattttattt
    14101 tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc ggattgattt
    14161 aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata ttgcgtagct
    14221 aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt tgctggtgag
    14281 aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac ctctttcatg
    14341 atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg ataaggcaat
    14401 tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac ttctcgcagt
    14461 gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt ttctccaata
    14521 ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt ttttccgtgt
    14581 tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa tgaattgagc
    14641 tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt cattaccgca
    14701 ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac ccctggaatt
    14761 gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc tgtttcatat
    14821 tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac cgatccataa
    14881 aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg tgcccagatc
    14941 gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga acgatataaa
    15001 acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat atgattgtcc
    15061 aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc gctaggtttg
    15121 ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt atatttgacg
    15181 agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag ttgttccagc
    15241 attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa tgtcgatccc
    15301 ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg aaggtgtgat
    15361 ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta aagacttccc
    15421 atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac cttctccggc
    15481 tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct gtccaacttc
    15541 tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat tctcagattt
    15601 caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga tccgcatgaa
    15661 attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc cttcatttac
    15721 tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc aatccgtatc
    15781 gccttctagc gctttgttga ataataccgt gaataattta tctctgctta tctccatacc
    15841 caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg aagagcagtt
    15901 attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt cgatttccgg
    15961 atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag taccagccga
    16021 aatgttccct gtccctttgg ctacactgtt cgtagcgacc ataccggttc ctgcatcccc
    16081 ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga tcagataagc
    16141 ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag gcaaaatgtg
    16201 atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat ttttcatttc
    16261 ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat ctcctacgcc
    16321 tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg tcaaaaaatt
    16381 aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag cgatactcca
    16441 tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt cttcagtgat
    16501 tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa atgctagata
    16561 accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct cgccatagat
    16621 ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc aaatatcttc
    16681 taatgaatag gtccagatac cattttctag tttgttttcc cactcataac taccagcggc
    16741 aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc caaactcaat
    16801 ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta cttccttcat
    16861 aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta gtagcgctta
    16921 caatgattat tgtatacttt tgtacgtaca tgtcaagcag ttatttacaa gaatacaata
    16981 aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca aaatctgatc
    17041 aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa aaaaagtttc
    17101 ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat atgatattgg
    17161 tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag gtaaagcttc
    17221 tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg gctctttatc
    17281 tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg cacttatttt
    17341 tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt ttctgattgc
    17401 agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc ctgcttatat
    17461 gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc aagtgatgat
    17521 tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagggt tgcctgaaac
    17581 gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt tatttttcgg
    17641 tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct tggaagaggc
    17701 aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat tcgaacaaat
    17761 ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt tgtttttaga
    17821 aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc aatttcaagg
    17881 agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg ggcatgcagc
    17941 gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg gttctttgct
    18001 ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag gcggtagcgt
    18061 gatgggactt tctttcatcc taccagctgt tttaggaact gtactggatg ctcatacaaa
    18121 ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt gcacatgggc
    18181 tccactgaca tgggtaatcg taggcgaaat attcccacta gctgtccgcg ggcgagcatc
    18241 tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc tttttcctgt
    18301 gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca tctgcttttt
    18361 aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt tagaggaaat
    18421 cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc attcttaaag
    18481 aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag tttccagtca
    18541 caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac ttgtgaccag
    18601 ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc cttgagcgat
    18661 gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga ggcggtaaaa
    18721 acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta caggttcata
    18781 gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc gtgcggcatc
    18841 ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc ctaaatggct
    18901 taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat agccattttc
    18961 tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga tgccatctaa
    19021 agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc gtgtttccgt
    19081 ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac acgcgctgat
    19141 aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga acaacagacg
    19201 ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca catcatctaa
    19261 acaaatataa gaggcattta actcttcata agcagcattg atcatcacca tcggaatgcc
    19321 ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac ttttcgtagg
    19381 ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact ctttctccaa
    19441 ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc gtaattcatt
    19501 ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg tgatgacacc
    19561 gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat ctacatatgt
    19621 gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga ttgcttgccg
    19681 aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg gaatggttgt
    19741 atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa tatcttgata
    19801 ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac gagtctgcaa
    19861 ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga acaggttcac
    19921 caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg tgccgattag
    19981 gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag atcaaaatat
    20041 caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat tgcttatttt
    20101 tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt aataaaagat
    20161 tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt gcactaccag
    20221 aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca tttactgaaa
    20281 aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc ccctttaacg
    20341 tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt tcaggatctt
    20401 gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg tctaaactga
    20461 aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc attggatcta
    20521 tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt atagttttat
    20581 gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact tcaggtgccc
    20641 agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg cttgcagcat
    20701 gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag cctggaaccg
    20761 atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct gcccgttgcc
    20821 gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca ttttctttat
    20881 tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat gtttttctat
    20941 tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat ttttgtccaa
    21001 cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga aatcatgttc
    21061 aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca gctgattgcc
    21121 gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac aatcataaac
    21181 agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata agtgttgcat
    21241 cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta ttaaaggtat
    21301 tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc gtaaaatagc
    21361 cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc aatcgatggg
    21421 actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat tccattcatc
    21481 aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat cacaagtagc
    21541 aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg tcgctgtatc
    21601 atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat caatatagtt
    21661 atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac gatcccattc
    21721 agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa ttgaatcatc
    21781 cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat aagcactctt
    21841 agcccccact tgccatggac cgtctagctc attccctaaa caccataatt taacgccaaa
    21901 aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt ttggaaaatt
    21961 acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat tcaccgtgaa
    22021 tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc caaaagaatt
    22081 tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt tcctacctac
    22141 agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa tgacacctaa
    22201 ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat cagctgtttc
    22261 atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa caaatgagcc
    22321 aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta ctttcacttc
    22381 ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag atacaagttt
    22441 ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga gcagtacaaa
    22501 tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata ctttccatgc
    22561 actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca catatatcca
    22621 ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg aaagtacgat
    22681 tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta acagactaag
    22741 gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa tgatatttcc
    22801 tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag caggcacgat
    22861 agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt aatcagaaac
    22921 cacaatcaac tgtccataaa gtttagtctc aactattttc atcttcctca ctctttcatt
    22981 ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac cactagtaat
    23041 ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat gttctgccct
    23101 gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt caatatcaat
    23161 ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga taaaagagta
    23221 ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa tccatcaatg
    23281 cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat catgaagata
    23341 ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag catgccaagc
    23401 aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat cattgtcagc
    23461 aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa tgcatacccc
    23521 gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat cactgtattt
    23581 ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc tagggtatag
    23641 ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt aaaagaagaa
    23701 ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat actaatcgtt
    23761 cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt aatcgagctt
    23821 tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg acatgtacac
    23881 attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca gtgctgatgc
    23941 atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa cagctgtttc
    24001 tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca cttgcatcga
    24061 accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag gtagcgtaat
    24121 gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat acaaagaggg
    24181 agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat tcttccaaac
    24241 agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt gagggatcgg
    24301 caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa gcaacataga
    24361 aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg tcgaacgaca
    24421 aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac caacgataat
    24481 ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat taactgctcg
    24541 ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg atgtaaagaa
    24601 aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga cagtaaataa
    24661 taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag gacgattcca
    24721 atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa aatatacaat
    24781 cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt tgcaataaca
    24841 cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac agtctatcct
    24901 gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg catcgttcat
    24961 gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga tgatatcgtt
    25021 attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt ccatttgttt
    25081 cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta ctgaatccaa
    25141 gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg tattccaata
    25201 tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg gaatggcata
    25261 tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg gtaaggtagt
    25321 cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc cattgatttc
    25381 aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa tcgattctgg
    25441 accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa ggttctcagg
    25501 agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat ttgctaacat
    25561 ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata catttttact
    25621 actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag gtggttgttc
    25681 agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca tgccttgtac
    25741 ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag ccgtttcttt
    25801 aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt gtaccgctcc
    25861 gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag gtgctttttt
    25921 agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga tatccatttc
    25981 aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt gcaatatttc
    26041 accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat cagaatcaga
    26101 ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta ccaacaaaga
    26161 gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat aaacgacatc
    26221 tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct tcagctttta
    26281 catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt gcttttgtat
    26341 caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca gacaagtgaa
    26401 cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt tctttgtcat
    26461 ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc gcatagtttg
    26521 ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct tgcataaatg
    26581 gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt ggtgcaataa
    26641 catttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc aataaagtga
    26701 ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct agtaaaggag
    26761 gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac caaatattcc
    26821 attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg actgtatcgc
    26881 acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca agataatgag
    26941 aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct atttgatcgt
    27001 acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc cgattcgaac
    27061 ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta gccgtttcct
    27121 ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga ccgtccattt
    27181 cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc gaacgcctta
    27241 aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta cgagctgcat
    27301 ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca tgctgacacc
    27361 agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa gctaaatcca
    27421 accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat cctgacacaa
    27481 aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag tgaatgacgt
    27541 cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc ccttcatata
    27601 ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact ttgcctacac
    27661 gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa tttatacatg
    27721 aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta cttatttttt
    27781 atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta atataacttt
    27841 tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata tatgcaacta
    27901 caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac aaggatcaag
    27961 atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa agaacttgga
    28021 gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa catcatcaaa
    28081 acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat cgatatcagt
    28141 tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc tattggtcac
    28201 tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga ttttatcggg
    28261 aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg cattctctgg
    28321 tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga ggatacattg
    28381 gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa tgacaattgg
    28441 ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac gagccctgga
    28501 gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa tcttaatcaa
    28561 tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa tggagaaccc
    28621 ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca tttgcgaatc
    28681 gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa aggattcggt
    28741 aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc ttttctattt
    28801 tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt attgttattt
    28861 ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct tgttccacca
    28921 tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat aatcatcaat
    28981 ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc catcttcaag
    29041 atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt gaagcaattg
    29101 tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac catcaccaaa
    29161 tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata cggctcctgc
    29221 tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga tatttccttt
    29281 gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg catcaacagc
    29341 tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag cagcttttgc
    29401 atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt gatagttttc
    29461 atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc ctgctaaagt
    29521 agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat attttttcaa
    29581 atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa ttgttggttt
    29641 gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc ctatgcttct
    29701 gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt atggttccag
    29761 cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt atactcttcc
    29821 catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc aggtaattcg
    29881 taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt aaagtctcga
    29941 acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc ttcttctcga
    30001 gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa atttcgagtg
    30061 atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct tgataacttg
    30121 atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa tactgcacca
    30181 agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac atcaatttta
    30241 gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga agcatagatt
    30301 tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga tatataggcc
    30361 atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa ttctttcgcc
    30421 acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac taagaacacg
    30481 cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc ataagattgg
    30541 actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc agcacgtaca
    30601 ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac tgctgcatcc
    30661 agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat gacagatttg
    30721 atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa cagtgctgtt
    30781 tctactaatt ggcagtgcgc taaaggccct tccacttgga ttaatggttc atttgcaaaa
    30841 actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt ttgtaaatac
    30901 tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc ggaatcattg
    30961 aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac cgcgtatccg
    31021 tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc agctcgacct
    31081 aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag cgtcaaactg
    31141 tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt catttcctac
    31201 ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg aaaaagcaca
    31261 tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat cctttcttat
    31321 tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag
    31381 aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct actacaatcg
    31441 tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa
    31501 tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa
    31561 acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac
    31621 cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag
    31681 aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt
    31741 tttttattgt atacattaga tatggagcag gttaccataa agatgattat tagtcgttat
    31801 taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg
    31861 aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct
    31921 cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc
    31981 ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt
    32041 cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact
    32101 actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc
    32161 atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct
    32221 ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt
    32281 atcacttaat agtataacat aatttcgtga gaaaaaaata caattttttc ttgaccatct
    32341 cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa
    32401 agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg
    32461 gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag
    32521 tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc
    32581 aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa
    32641 tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg
    32701 atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac
    32761 aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa
    32821 aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa
    32881 tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag
    32941 atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttctct tacagctggc
    33001 ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt
    33061 tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta
    33121 tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta
    33181 ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat
    33241 tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg
    33301 tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc
    33361 ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg
    33421 tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat
    33481 atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt
    33541 tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag
    33601 ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa
    33661 cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga
    33721 ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac
    33781 tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat
    33841 gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt
    33901 atgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat
    33961 cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct
    34021 tttcgttttt ccataactta actgtctggc atttgcatta gggatataat cgacttcttt
    34081 catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac
    34141 acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac
    34201 tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg
    34261 cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa
    34321 tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta
    34381 ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa
    34441 aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct
    34501 tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa
    34561 agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat tgaatacatt
    34621 ataatgatac tgtaaatata tctttattat tagtgaat
//
LOCUS       DASOUD010000019        33764 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.019, whole genome shotgun sequence.
ACCESSION   DASOUD010000019 DASOUD010000000
VERSION     DASOUD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.019"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            58..1956
                     /locus_tag="U4A29_000854"
     CDS             58..1956
                     /locus_tag="U4A29_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO0679995.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2255..2974)
                     /locus_tag="U4A29_000855"
     CDS             complement(2255..2974)
                     /locus_tag="U4A29_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO0679996.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3311..3643
                     /locus_tag="U4A29_000856"
     CDS             3311..3643
                     /locus_tag="U4A29_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0679997.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3630..5633
                     /locus_tag="U4A29_000857"
     CDS             3630..5633
                     /locus_tag="U4A29_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEO0679998.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5652..6122
                     /locus_tag="U4A29_000858"
     CDS             5652..6122
                     /locus_tag="U4A29_000858"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEO0679999.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6143..6727
                     /locus_tag="U4A29_000859"
     CDS             6143..6727
                     /locus_tag="U4A29_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEO0680000.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6742..7728
                     /locus_tag="U4A29_000860"
     CDS             6742..7728
                     /locus_tag="U4A29_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEO0680001.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7718..8029
                     /locus_tag="U4A29_000861"
     CDS             7718..8029
                     /locus_tag="U4A29_000861"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEO0680002.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8051..9832
                     /locus_tag="U4A29_000862"
     CDS             8051..9832
                     /locus_tag="U4A29_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEO0680003.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9825..11201
                     /locus_tag="U4A29_000863"
     CDS             9825..11201
                     /locus_tag="U4A29_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEO0680004.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11208..11843
                     /locus_tag="U4A29_000864"
     CDS             11208..11843
                     /locus_tag="U4A29_000864"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEO0680005.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11873..13228
                     /locus_tag="U4A29_000865"
     CDS             11873..13228
                     /locus_tag="U4A29_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO0680006.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13420..14814)
                     /locus_tag="U4A29_000866"
     CDS             complement(13420..14814)
                     /locus_tag="U4A29_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO0680007.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14906..15877
                     /locus_tag="U4A29_000867"
     CDS             14906..15877
                     /locus_tag="U4A29_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEO0680008.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15874..16655
                     /locus_tag="U4A29_000868"
                     /pseudo
     CDS             15874..16655
                     /locus_tag="U4A29_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(16824..17195)
                     /locus_tag="U4A29_000869"
     CDS             complement(16824..17195)
                     /locus_tag="U4A29_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO0680009.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            17329..17772
                     /locus_tag="U4A29_000870"
     CDS             17329..17772
                     /locus_tag="U4A29_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO0680010.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17815..18624)
                     /gene="yidA"
                     /locus_tag="U4A29_000871"
     CDS             complement(17815..18624)
                     /gene="yidA"
                     /locus_tag="U4A29_000871"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO0680011.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18643..20010)
                     /locus_tag="U4A29_000872"
     CDS             complement(18643..20010)
                     /locus_tag="U4A29_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO0680012.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19994..20824)
                     /locus_tag="U4A29_000873"
     CDS             complement(19994..20824)
                     /locus_tag="U4A29_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO0680013.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20987..21424
                     /locus_tag="U4A29_000874"
     CDS             20987..21424
                     /locus_tag="U4A29_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO0680014.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21500..22120
                     /locus_tag="U4A29_000875"
     CDS             21500..22120
                     /locus_tag="U4A29_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO0680015.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22309..22815
                     /locus_tag="U4A29_000876"
     CDS             22309..22815
                     /locus_tag="U4A29_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEO0680016.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22832..23731
                     /locus_tag="U4A29_000877"
     CDS             22832..23731
                     /locus_tag="U4A29_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEO0680017.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23818..24702
                     /locus_tag="U4A29_000878"
     CDS             23818..24702
                     /locus_tag="U4A29_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO0680018.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24894..24965)
                     /locus_tag="U4A29_000879"
     tRNA            complement(24894..24965)
                     /locus_tag="U4A29_000879"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24930..24932),aa:Arg,seq:ccg)
     gene            25025..26359
                     /gene="rpoN"
                     /locus_tag="U4A29_000880"
     CDS             25025..26359
                     /gene="rpoN"
                     /locus_tag="U4A29_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEO0680019.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26508..27599
                     /locus_tag="U4A29_000881"
     CDS             26508..27599
                     /locus_tag="U4A29_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEO0680020.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            27640..28641
                     /gene="gap"
                     /locus_tag="U4A29_000882"
     CDS             27640..28641
                     /gene="gap"
                     /locus_tag="U4A29_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO0680021.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28751..29941
                     /locus_tag="U4A29_000883"
     CDS             28751..29941
                     /locus_tag="U4A29_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO0680022.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30045..30800
                     /locus_tag="U4A29_000884"
     CDS             30045..30800
                     /locus_tag="U4A29_000884"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO0680023.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            30916..32214
                     /gene="eno"
                     /locus_tag="U4A29_000885"
     CDS             30916..32214
                     /gene="eno"
                     /locus_tag="U4A29_000885"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO0680024.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            32613..33752
                     /locus_tag="U4A29_000886"
     CDS             32613..33752
                     /locus_tag="U4A29_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEO0680025.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        11158 a         5983 c         7196 g         9427 t
ORIGIN      
        1 aaaatagagc ataacaactt tataatgttg agtataccaa tttttggagg cgttttaatg
       61 gaccgctatt tagatttact ttcagagaag ttccctacaa cggaagatgt catcacggaa
      121 atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagcgat
      181 atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa
      241 gaaaagatca aagacctttt ctctgaacaa atgacggaaa cggagatcag ccaattcact
      301 atttttacgg cttatccaga atatgtgttg acgactgatt ggtacaaaaa acaagataaa
      361 tcccagctga tccatcgttt gatcgatctt ttacggttta cgacagtcaa atacactcga
      421 tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat
      481 atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc
      541 ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc agacgatcca aaaatctgtc
      601 atcgatcatt tgcatatcgt gggtgatatt tttgatcgag gaactcaagc agccaaagta
      661 atggatcgaa tcatggattt ttcctctgta gatatccagt gggggaatca tgatattttg
      721 tggattggtg cgtattgcgg ctctgaggca tgtttattga cgcttttacg gattgccgca
      781 cgctacaact atttatttga attagagaaa gaatatggat taaatttacg tccattattt
      841 tcttttgccc atcaaaccta tcagaagaat ccagtattta cccctaaaaa caggaaagat
      901 ctatctgaaa gagagcaaga agaattagaa aagatccatc aagcactttc tatcctccaa
      961 ttcaaatcag aacatcagtt gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa
     1021 ttgttggaaa aaatcaacta tgaggaatca acgatcgttt tagaaggtca gctctatgag
     1081 ttgaaaggga cttgttttca aacgatccag ccagatgatc ctaaaaaatt cttggaagag
     1141 gaacaaaaag tgatcgatag tttgatgtat tcattccagc actcgttaag aatgcaaaaa
     1201 cacatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtactg
     1261 ttccatggct gtatacctgt agatgaaaag gggcagctct tagcatttga attagaccaa
     1321 tcttatcgcg gaaaagaact gctgggattt tttgaaaaac agataactga aagcgcaaaa
     1381 gatttgactg caaatgaaga tttggcgacc gatttgattt ggtatgcgtg gagcgggcca
     1441 ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg agcgctattt tctcacagag
     1501 cctcatacgc acgtcgaaaa aagcaatccc tattatcact tgcgagatga agagtggttc
     1561 agtgaaaaaa ttttggcaga attcgatgca aaagaagaag gttcagcaat catcaatgga
     1621 cacacgccag taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg aaaagcattt
     1681 gttattgacg gaggtctatc caaagcttat caaaaaacaa ccgggattgc tggctactca
     1741 ttactatgca attcttacgg tttccaaatc gtgacacatt atccgttctt gcctattgaa
     1801 caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc
     1861 ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca gattactcaa
     1921 ctccaaaggc tgttggatta tcgaaaggac gattgagaaa aatgctagcc aacattgctc
     1981 aatagactaa aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg aattagctca
     2041 aaaaacggta aaatattaga agctattttt ctgtttattt ctctaatact caggtcaggg
     2101 cgctgatttc acgacctctt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt
     2161 cacaatatcc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg actcagagtt
     2221 gaacggcagg ctcacgacct aatcagaaaa acgattaaaa ttcattcaca aagaaatcga
     2281 ataaacgctg ttcagatgaa tctacacgat gagaaagttc cggatgccat tggacaccaa
     2341 ggattttgtg catattatct ttactttgga ctgcttcaat gagtccatcg ttggaaaagg
     2401 cgatggcttt taacaaagga gaaagatctt tgactgcctg atgatgatag gaattcacaa
     2461 agtatgaatc agggagaagg tcacttaata aactgtccga aacgattttg acttcgtgag
     2521 ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac ggaccatttt ggatataatg
     2581 aaagatcttg ataaagtgtt ccctccaaca cgacattcaa tagttgaagt cctcggcaga
     2641 ctgcaaaaat cggttttctt tgtttcaatg cttccaagat caatgcctgt tcgaattggt
     2701 ctcgttgaat atttgtttcc gtcagttttg gatgagggtc ttccatgtaa agttgcggac
     2761 taacatcctg accgcctgca agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg
     2821 aatctggaga catgactggc agaataagag gtaatccttg agcatctaaa actgcatcca
     2881 caaatccctg cggtgtatag cttacttgat ttccttggaa agtatctgtc gctcgaatca
     2941 gttgattccc ggcaatgccg atgattggtt tcatttttat tccccctaaa gtagactaat
     3001 tgtaacaagg atttaaaaaa atttaaacta tgtaagactt gcccctatac ttatctgttc
     3061 ttgctgtgtt actatctgta aagagaattt tcgtgtgaaa ttcatgacga tagttgaaaa
     3121 tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc
     3181 aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct gcctatacta
     3241 agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct cttttttaaa
     3301 tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag cagaagaaga
     3361 aaatgagaag cgtgctcgaa ccctacggca gcagttgaag acctatgcag aagaaaaaga
     3421 gcaagaattg gagataatcc aaacgaacag tcggcaacag ctgaaacagt tggtagaaga
     3481 aaaagaacaa ttcgaaaagc aagaattaga aaaagaaaaa atgaaattga aagcagaagc
     3541 agacaaggtg caagcaactt tgaaagacca atatcaaaag catcagagtc aagcaatcgg
     3601 tgcaatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa aaagtgaccc
     3661 tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga atccaaaatg
     3721 ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtagaa aaatattttc
     3781 cagataccac gattattgat aaagaagcaa aattaagcgc tttaggcaat cggctgaaag
     3841 acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa aagaaactcc
     3901 atttgaaacg tcaagaactt tccttacaag acttggaagc aaattattcc gaagaaaatt
     3961 tcatgaataa attagaagag gtccttgatt taaaagaaaa gtgggaacag ctaactgaaa
     4021 aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag ctggatattg
     4081 ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg agtgcagcaa
     4141 attttgaaag tttcaaggaa gaactaagta agcttgatga cgtttatatg gaagaaatag
     4201 attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca gttgctgcta
     4261 tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat gaacaaaatc
     4321 ctaaagaaaa attgcaagaa accaaacagc aattatccat tgttcacgag cagcaaaaaa
     4381 agataacgac agcgttaggt cagtgcagcg gctatatcca agaatttgaa tgggtagaag
     4441 aagtcacact tgccgttgca gaacgggaaa aagtaaaaga acggtttatc catgccactt
     4501 atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg atgcatgtgt
     4561 taaacgaaac tgtatcagaa gaagatatat acgttagttt cgaaacacca aactcagaag
     4621 agattcagca ggaagtccct actaaattga aaaaccatcc gcttatcgaa ccttttgaat
     4681 tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaacg ccttggatga
     4741 caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttac ggattgctga
     4801 tgctcattgg gacaatacta gctcaaaaac tatttgtttt accccgcggg atgaaacgtt
     4861 tcgtaaaatt ctttgagatt ctctcggtac catcgatcat ctggggattg atctacagtt
     4921 catttttcgg acaggcatta ccaaaagaac tatttggtat ccgcttgcct ttcccaatcc
     4981 tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc ggtctgatac
     5041 agatcttggt tggattattc gtggcagcaa aggaaaatat caggaggaaa gattacttga
     5101 gtgctgtcag tgatggtttt gcctggcaag ggatcctgat tgggatcgtt attgcattga
     5161 ttggtgtcat gttgataaaa aatacagcat ttgtttatct tggcgcgggg ctatcgatca
     5221 ttgctgcttt atgtatctta atcgtgccga ttatccaatc gccgtctaaa gtcaagggtg
     5281 ctgccaaagg agcgtataat ctttacggat tgactggtta tatcggtgat ttagtcagtt
     5341 atacacgttt gatggcacta ggtatttcag gcggaagtat tggcgcggca ttcaatatgc
     5401 tagttgcttt catgccgcca gccgctcgat tcagtgtagg gatccttttg atcatcgctt
     5461 tacacgcact gaatatgttt ttgacactgt taagtgccta tgtccatggt gcacgattgc
     5521 agtatgtcga attttttgga aaattttata caggaggcgg acgtgccttt gacccgctga
     5581 aaacggcgga aaaatacgtc aacatcaatc ataagaaaaa aaacaataaa taattggagg
     5641 atttttatac aatgatggat tacttaatta gtcaaaatgg tggaatggta tttgcagtat
     5701 tagcgatggc aacagcgacg attttttcag ggattggttc agctaaaggt gttggaatga
     5761 caggtgaggc agcagcagcg ttaacaacaa gtcaacccga aaaattcgga caggctttga
     5821 ttctgcaatt gcttccaggt acgcaagggt tgtatggatt cgttatcgcc tttttgattt
     5881 ttatcaactt aggaaatgat atgtcagttg tacaaggatt gaactttcta ggcgcttctc
     5941 tgccgattgc ttttacaggt ttgttttcag gtatcgccca agggaaagtt gcagcagcag
     6001 gtatccaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt tttgctgcga
     6061 tggttgaaac atatgcgatt ttaggtttcg ttatttcctt cctattagta ttgaatgcct
     6121 aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc agatgaatga
     6181 agcagcagag caagaaagag cggcgttaga gcaagaagag cgaatgaaga tcgatcaaaa
     6241 ctttgaacaa aaacgtacgc aaatcgaaac agaacatcaa aaacaaaaag aaaaacaaat
     6301 cgaattactt gaaaaaaaat accgtcagct taggaatcgc caacaagtag aagtacgaca
     6361 agaaaatttg aatgcgaagc aggaattttt acgacgattg tttgcagatg caatagctga
     6421 aatggagaat tgggatgaat ctgaacaaat ccagtttatc aaaaattcat tgtattcatt
     6481 acctttgaca ggcaaagtaa cattcattgc tggagaaaaa tcagcagctt atctttctca
     6541 gacattgctg gatgaatgga acaatgaact tccctttatg atggttttaa gtgatgaaac
     6601 tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata attttctttt
     6661 cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca atcaattatt
     6721 cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat tcgcggaaga
     6781 gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa
     6841 gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga cggatatgaa
     6901 tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg gttaaaggaa
     6961 tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gctatacttt ccataatctg
     7021 aaggttttga caaaagccga gatgacaaat caaaatctgg accatttgta tatcgacgat
     7081 ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc aaatgaactc
     7141 cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga gtctaccatt
     7201 cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg acggcttgcc
     7261 gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga cttgaccaat
     7321 atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat gacgacggta
     7381 ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga taaagatatg
     7441 gaagattaca cgaattttct attagagaca gattatgctg attttctgcg ccctgtcatc
     7501 cgtgagggaa atcttgatct cgtaaagctt gaacagttga aagatgatta tctaagttca
     7561 ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc ctttttgaac
     7621 gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag aagccatttc
     7681 gaaccagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga tcggtgtagt
     7741 tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc gttatggtac
     7801 aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt atggcgtgat
     7861 ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt acaaggaaca
     7921 aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga tagggctgga
     7981 agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt atgaaaagga
     8041 gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg taatggctga
     8101 gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag gagttatcgg
     8161 agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag agacttctgg
     8221 aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg aattaggacc
     8281 agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct ttatggaaat
     8341 cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc atgagaaaaa
     8401 atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag atgttatcgg
     8461 gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg ggataaaagg
     8521 tacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat gtgtcattga
     8581 aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg
     8641 gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc gagtgattga
     8701 tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt ttggtgcagg
     8761 gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg ttgtctatgt
     8821 gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc ctgaattgat
     8881 tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta atacatcaaa
     8941 catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg cggaatattt
     9001 ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat gggcagaagc
     9061 acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt atccagctta
     9121 tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg cattaggatc
     9181 agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tcgccgtctg gaggggatat
     9241 ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg gtcttgattc
     9301 cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct attctcttta
     9361 ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg catccatggt
     9421 caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt
     9481 agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa aatccattcg
     9541 tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt caagagagaa
     9601 acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc
     9661 gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg aaaggatcgg
     9721 acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta ttggtgaaat
     9781 caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt aaagaatatc
     9841 gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct ggtgtcaaat
     9901 atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg
     9961 aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt atcaatttac
    10021 gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg gaagacatga
    10081 tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag atcctgcctg
    10141 aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat tatccagatg
    10201 agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa
    10261 aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct cagatcgcgc
    10321 gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct gctattggaa
    10381 tcacttttga agaagcagaa tttttcatgg aagattttcg tcaaacgggc gcaatcgacc
    10441 gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgagcgtatc gctactcctc
    10501 gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat gttttggtta
    10561 tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca gcacggcgag
    10621 aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct actttattcg
    10681 aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg attctaacaa
    10741 tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt acagaaggac
    10801 agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt gatgtactcc
    10861 cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga gaagaccatg
    10921 cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca aaagaattgg
    10981 ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg aagtttgctg
    11041 agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg attactgaaa
    11101 cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta aaacgaatca
    11161 aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg gcacgattga
    11221 atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca actgccacaa
    11281 gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc atcctgttga
    11341 ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg
    11401 attttgtatt ggcaaatgct tcgatcacgg agcctttcat cgaagaatta tttgttttgc
    11461 cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta aaagtacctg
    11521 tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac gggtatttga
    11581 attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt ccaaaacttt
    11641 tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc gaaaagacac
    11701 gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa acgatctatt
    11761 atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa
    11821 aaaatatggg taccactgac taagtggtat ccttttttaa aagaggaaaa cgatgattaa
    11881 acaaagcaaa caaataaaga aacgcttgcc ggctgttcag cttctcgcat taggattttt
    11941 tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta atagtgggca
    12001 tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg tgacgggatt
    12061 gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga tcatgctttt
    12121 gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg cattgataaa
    12181 gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc tagaagaagt
    12241 atcaggtgtg atgaagctca tgctttacat cctgaaattg gcctttgtga tccaactgat
    12301 tggtacggtt gctttatctt ttgtattcgt gcctgaattt ggctggttga aaggtatctg
    12361 gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt tgttaggaga
    12421 cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg ctttgatcat
    12481 ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc agaaaaagaa
    12541 gataacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat taggcggatt
    12601 cgttgtgttc tttttcacag agcgcaatgg ggaaacgcta gtggaaggga atatgtttca
    12661 acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat attattcagt
    12721 tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga tgtacatcgg
    12781 tggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg tgttgctgat
    12841 ccagatgcgt tcgatgctta aaggacgtac gagagcggaa gcatttggcc gtaccatccg
    12901 acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt tgtgtgtagt
    12961 cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca tcgaatatat
    13021 tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat tgacgccaga
    13081 cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac gagttggtgt
    13141 attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa aatacaaata
    13201 tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct agtgatttag
    13261 taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg caaaatgttc
    13321 gtttattttt tgaggcacta tccgttgttg aacctcgggt agtgcctctt tcttattgaa
    13381 aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat atttttttat
    13441 tttttctttt acgatactta gtccagaagc taaaatttca aggttattag aggataggga
    13501 aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg
    13561 gataccgtta gcaagaaagt cattctcaat tatcttagga tcaatctcag gatcaactgg
    13621 aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa gagaatcaaa
    13681 aagctgattg gctcttttcg taagacttaa tttagtttct aagatcctat ttgcttcatt
    13741 tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca catttatatt
    13801 aaaaattgct tgttctatct tgttttggac atgtactgga actacaagat aagcaactcg
    13861 caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt taggtaataa
    13921 tttttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg aatggatatc
    13981 atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct ctcgtctttt
    14041 gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga caccatgaat
    14101 cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct cgtcaaaatc
    14161 aattggaacg atctctaaat gatataactg agccaattca atgaaattag cgtatgtata
    14221 tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga tagccaatcc
    14281 attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc cttgagtttt
    14341 taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt ggtaattaag
    14401 caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa cgaatttatt
    14461 acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta cttcattggt
    14521 agagatggtg atggactcaa agacattggg agaaataaat gtgccacttc caatattcgt
    14581 atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag taaaatttaa
    14641 atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct gtaatttatt
    14701 ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac gcttcaattt
    14761 ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag gcattttcta
    14821 cactccttaa aattgtatac catacaattt taccattgat tgtattgatt ttagaagtta
    14881 atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag tagtagcttc
    14941 ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg
    15001 taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag acaaaaatat
    15061 gtttgcgatg atgcctgctt atttggggga gaacagatat tttggaacaa aagtcattac
    15121 cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga ttttcttgtt
    15181 tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca cctggattcg
    15241 aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg cagaaacact
    15301 tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt ctaccattcg
    15361 tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata cttttatcgc
    15421 taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag tagaagatgc
    15481 agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa
    15541 agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc ctgatacaag
    15601 agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt actcagctac
    15661 cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag aggaatcttg
    15721 tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata aaggagaatc
    15781 gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt gtgcagaata
    15841 tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta cgtagttgat
    15901 gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct agaagaatgg
    15961 ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga aacagcattt
    16021 acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg agaaatcgat
    16081 ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta caaaatacct
    16141 gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag aattaaagat
    16201 tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt gactgaatat
    16261 gaagaagcaa ttggggctaa gattaaagaa gcctacttag cacgtgattt atttttcgta
    16321 ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat caaaaatttt
    16381 gaattaggaa tagagtcatc gtgacagcag aaagtgtgca aaaagacttt gtttctagaa
    16441 ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca cattctaatc
    16501 ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat caaatgtcat
    16561 caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata ggaggaaaag
    16621 cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa ttaaaaataa
    16681 attggtggag agtggcagcc ttatagctac aatggttgtt aaaaaaagac gcaaactttt
    16741 tcaatttaat gaaaagtttg cgtctttttt taattattgg tgtttgtttt ttattcaatc
    16801 aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata accatcaggg
    16861 tcaatgacaa agtagtaaga aggcgtagtg ccaggtaatc cttttaagtc agtaatctca
    16921 agacctgcag ctttatgttg ctcgtgtaat ttttctaaat catcagctgc tatagcgata
    16981 tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt caactctaat
    17041 tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc tggaaaatca
    17101 cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga tgcttcaaga
    17161 tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct tttcctttta
    17221 ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca tgagaaattt
    17281 ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat ggataaacca
    17341 atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc atatattgtc
    17401 atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt ttttcttcct
    17461 ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga gctgatagaa
    17521 gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga gtatttttac
    17581 tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc tgattcttgg
    17641 aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa
    17701 gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg gatcgaaaaa
    17761 aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta tttattacag
    17821 tacatatttt ttgattgctt cagctacacc gtgctcgtcg tttgatgaag tgatgacatt
    17881 tgctgcttct ttgacaagag aaacggcatt tcccattgct actcctagac cagcatattc
    17941 aatcatggat aagtcgttct cgttatctcc gatagccatg atttcatctt gatcaatacc
    18001 aaggtgctgt gcgagattcg caactgccgc acctttactt gcttctttat ttagtacttc
    18061 gaagtaatag ggcgcacttt tgaccgttgt gtatttatca cggaaagttt gcggtaaacg
    18121 cgcaattgct gcatctaaaa tttctggttc gtcgatcatc atcattttga cgatgttcat
    18181 gtctggtgtc atttcttcag gtgttcgata ttttagcggc atcttgacta aacttgcttc
    18241 gtggatcgta taagcgctga tatctcgatt aggcgtgtag atcgctttgt catcgatcga
    18301 atggagatga ctgccgacac ggcgtgccat cacttcgatc tctaggaaat cttcatgtgt
    18361 cagtccgtga cgggcgatga ttttacctga tttcgtttgt tgtacgagtg ccccgttata
    18421 agtaataacg taatcattat cttgaaaaag gttcaactcg tttaactgtt cctggacacc
    18481 tggtaaggga cggccagtgc ataaaacgat cgaaacacct tttgcaacag cagcggcaat
    18541 cgcttctttt acttcttttg ttacttctct tttctcattt aataaagtac catcgatgtc
    18601 gatagcgact agtttgatag acataatttc ctccaatata ttttaattta tttcaatcaa
    18661 tgcgccatta cggatatggc tggcaaattc ttgataagtt tcgtcaaata aatcataatg
    18721 atcacgcaag gacggatcaa ccatttcttt tggaaagaaa aatcgctcat ctccttgttc
    18781 ttgtcccgct aatgcagcaa caagctgact aacctgtgat aattctatca aagttccgtc
    18841 atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata
    18901 aggtagatcg tagctagaat tcacagcagt gtaataaaca ggattatatc cgaccttttc
    18961 tactaacaaa gtgagttctt ggactaattt tgaatcccgg ttgccagaaa aagtagcaga
    19021 tttcagtggt ttacgattca aaaaacgttt agctaaatca tttaaaatag gatcagcaga
    19081 ttcagaccat tgagtaaagt aagtgcccaa gaccccatca tcaagtttga ggtattcttc
    19141 taaagtaaag cttccgtcaa gaaatggcag aagtaattga gaatggagtt caaaactgct
    19201 ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat
    19261 accacgggaa acaggatgga agtacacttg cacatacatt tgatatcggc taacgatata
    19321 atcttccact gcatgcatgc cactcatgga aaaagcgatc ccgccttcat aaggacgaat
    19381 cacacgtaat attcgggtaa gatcaaatgt tccatattct gttccagtga aataagcatc
    19441 ccgaagcaaa tagtccattc gatctgcatc gatctgacta gaaatcattt ggacaacttg
    19501 tgggttgggg taagtttttt ggatcacact agctaccttt tcaggaaaac cttcttctac
    19561 tcggttaaga atctgataca cttctgtttc tggagaagtg atgatctcta cagtgatggc
    19621 ttcatgattc gtatggaaga tatgttcaaa tgtatgagag taaggaccat gaccgacatc
    19681 atgcaacagt gccgcacata aagtcaccag ccgttcctga tcatcccagc ctccgttgcc
    19741 tattttttct tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg aaatctcata
    19801 aacgcctaat gaatgagtaa aacggctatg ttctgctccg tgaaaagtga aagaagaagt
    19861 gcctaattgt ttaatgcgtc gtagacgttg gacttctttc gaattgatca aatctaagat
    19921 tacttgatgc tggacatgga tatagttatg gactggatca cgaaaaactt tttccatagg
    19981 cagcatttga tatttataag gggttgtcat aatgtagctc ctttatgtta ttacgttgtt
    20041 ccattctctg gatttggttg ttccattgct ccagctggat ttcttttttt agccagtcgg
    20101 tagaatcctg taaatcactt gcagaataca gcttttcaaa agcttttgca aatgcttctt
    20161 tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc attgtagcag
    20221 gatccactgc aggatagcca tttgtaccaa agtcttcttt taaagcagat tgataaaatc
    20281 gcttcatgac ttgcccgcgt ttttcctgat taccgttgac acttaagtac atcataacag
    20341 aaatcccatt tttgatccga cgctgagcga taccggcaaa ttttttgcca tggatgctta
    20401 aatcataagt tccaggacaa taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg
    20461 gaaaagcgta tttagtcaat aggagcattt gctcgtaagc gtcatctatg gttatttttt
    20521 ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat tccttcatct gatacgatcc
    20581 ctaatccacc tgaatttctg atcactggag cgtaacctgc atctttcagt gtttggatcc
    20641 cgtcacttag aaaaggagtt cgaacatcct tcatacctaa gatgactgtg cagtcatatt
    20701 gccagaaatg taaaaagatt tgctggtgtt ctttagaata ttccgttaat atatctgtta
    20761 atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg atctagtcga atcgcagggt
    20821 tcattcaacc ggctcctttg tataaattca tattttctac aatattatac tctaaaatca
    20881 aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac aagttggttg acaaaacaaa
    20941 agaaactagg aacaatgtag ctatgagaag taaaggagaa tgaataatgg aattatcaca
    21001 aggaacaccg atttcaatca aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga
    21061 tttttatttt gatttgaaag ggcaaatggt gacaattggc gatacgctgt acatacgtta
    21121 taaagagatt caggaggaga cgggcgatga gattcctgta acgatcaaat tagttccaga
    21181 tggtcatgta cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat ttggctataa
    21241 agaacggtta gaaacatcct atcgaacacc atacggtatg atccagattg ctacttttac
    21301 gaaagaactg catgttagtt tgaaagaccg tcccacagga gggaaggttc gaatcgatta
    21361 tgatctgttc atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg attttacagc
    21421 gtaaatataa aagaaaagtt tgattttttg tgtctgattt tgtatgataa ggagtagact
    21481 gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt
    21541 atcaatgatc gaagtagcac acgcgatctt agaacaacgt ggcgatgtca tggatttttc
    21601 tgatttagct aaccagatcc aaaactatct tgggaaatca gacagtgaaa ttcgtgattc
    21661 actcgcacaa ttttatactg acttgaatat tgacggcagc tttatctcat taggggataa
    21721 tcgttggggc ttgcgttcat ggtatgctat cgattcgatc gatgaagaag taaaccatgg
    21781 aatggacgaa gaggatgaag atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt
    21841 tatcaatgat gatgaagacg cgatcgatta caatgacgat gacccagaag atacagatct
    21901 gacagaagaa gatgacgatg atctgtttga tgacgatgat gatgaagatg aagaaatcgc
    21961 tgcttataat tctgacttgc aagaaatcgg agcagatgac ccgacagatg atgaagaaga
    22021 cttgccagga aatatcgaag aagatttaag tatcatagac gaagacgatg atgatgaaga
    22081 ttatgaagag gaagaatttt ctgactctga aaaagaataa acattatttt cttacgagag
    22141 gttgtgacag agacgatcag cctatcagaa taaggaggtg tgacacgatt tttgtcacac
    22201 cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt
    22261 ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact
    22321 ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa
    22381 actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc
    22441 ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag
    22501 atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg
    22561 cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta
    22621 cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa
    22681 aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc
    22741 ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt
    22801 caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa
    22861 actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca
    22921 gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca
    22981 agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag
    23041 tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt
    23101 aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac
    23161 acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg
    23221 acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca
    23281 agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca
    23341 gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa
    23401 aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt
    23461 gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta
    23521 ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac
    23581 acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat
    23641 tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat
    23701 tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca
    23761 attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg
    23821 aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt
    23881 acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg
    23941 tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct
    24001 aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca
    24061 acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt
    24121 gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac
    24181 gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag
    24241 cagacagctg aagcagggca cgagattggc aattacacat acgatcatca gaatctggcc
    24301 actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa
    24361 gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
    24421 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
    24481 agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt
    24541 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
    24601 aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc
    24661 gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta
    24721 ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa
    24781 caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg gacagttttc
    24841 acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc
    24901 tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag
    24961 gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa
    25021 aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat
    25081 gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc
    25141 attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca
    25201 gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa
    25261 tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa
    25321 ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa
    25381 cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca
    25441 aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag
    25501 tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta
    25561 tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca
    25621 acgtttttct gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag
    25681 tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg
    25741 ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagccaaa
    25801 tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga
    25861 aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctacaacg
    25921 tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat
    25981 cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt
    26041 gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag atttcggtgt
    26101 atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga
    26161 agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa
    26221 aaacaagccg ttgtcggacc agaaattggt tgaattgctg aaaaaagatg agatagcgat
    26281 ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag
    26341 aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact ttttctagtc
    26401 tttttagttg aaaaacgaga gcgaaactgt tagacttaca gtcgtgaggt tgagatttcc
    26461 ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg ggtcgtttta
    26521 agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat gaaataaaaa
    26581 tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat aaaattttac
    26641 gaaatatcta ttggatgcag ccgattgggc gtcgaagttt atcggaaagt atggggatca
    26701 cagaacgagt cttacgaaca gaaacagacc ttttgaaaaa tctgaatttg atcgacacct
    26761 caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc cttgaaaact
    26821 tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa tattttggta
    26881 accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat
    26941 tcggcgaagc gctgagcgaa gccttagacc gactacttcc agaaggcgag aatatcatcg
    27001 ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat ctggaaaata
    27061 agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg ataactgtac
    27121 aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac cgcgcacttt
    27181 atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag ccatctatcc
    27241 aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt gggcgtgcat
    27301 tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg aaacaagcaa
    27361 atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc gtttataagg
    27421 tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg
    27481 cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa
    27541 cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta accctttaaa
    27601 ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa agtaggtatt
    27661 aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt agatggaatc
    27721 gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt gttaaaatac
    27781 gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc attcaacgta
    27841 aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc atggggcgaa
    27901 ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc agcagctgaa
    27961 aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt
    28021 ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac agttatctca
    28081 ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa cgacaaattt
    28141 ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca aatgactcta
    28201 gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt
    28261 cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcccgaatt gaacggtaaa
    28321 ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga attagtaaca
    28381 gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga agcttcaaac
    28441 gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg tatgacttac
    28501 ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa acaattagtt
    28561 aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt acgtacttta
    28621 gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata gacatgtaca
    28681 tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag tttattcagg
    28741 aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca aaaaagttct
    28801 tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg acactcgtat
    28861 caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag caatcctttt
    28921 ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt tagctccagt
    28981 tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg
    29041 agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa
    29101 tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag aactaggtaa
    29161 atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag ctcaccgtgc
    29221 acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg gattcttgat
    29281 ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac caatgattgc
    29341 aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact tgattcctaa
    29401 agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag caaaaggaat
    29461 caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag aattaatcga
    29521 aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa
    29581 tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg ctttagacat
    29641 cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa cagttgtttg
    29701 gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa tcggtgtttg
    29761 tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag actcagccgc
    29821 agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag gtggcggcgc
    29881 aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca acgataaata
    29941 aacgaccaag tttgctcaat tctgacgagt aatcgaacaa aaggtgaagg gagttttcat
    30001 gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt aaacctatta
    30061 tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt gctgaagcag
    30121 taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct cctgcattat
    30181 tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca gcacaaaact
    30241 gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct cttgctgatc
    30301 taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc catgaaacag
    30361 acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca ccaatcctat
    30421 gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg atcgaaggac
    30481 aaattacagc tggtttagct gatctttcag ctgaacaggt gtcaaacatg gttattgctt
    30541 atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc gctgacgaaa
    30601 tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta tcagaagcag
    30661 ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac atggcaaaag
    30721 aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca ttcttgtcat
    30781 tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact
    30841 aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa aacaaactca
    30901 aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat cttagactca
    30961 cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt tggccgcgga
    31021 atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg tgacggagac
    31081 aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa caacatcatt
    31141 gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc
    31201 gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt aggtgtttct
    31261 atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca ctacctaggc
    31321 ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg cggatcacat
    31381 gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc tccaacattc
    31441 aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat cttgaaagct
    31501 cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct tggttcaaac
    31561 gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt acctggtaaa
    31621 gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga aaaaggtgtt
    31681 tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat caaattctac
    31741 gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga cgaaaacgac
    31801 tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt agttggtgac
    31861 gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac
    31921 tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga agctatcgaa
    31981 atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga aacagaagat
    32041 tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac tggttctcta
    32101 tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga ccaacttggt
    32161 gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa ataattcttt
    32221 tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac ttagttaagt
    32281 aatcgaactc tctacttgct gaaatatcag caggtagaga gtttttttgc aactagcttc
    32341 tgtatatgat gtttacaaag aatggtacaa gaaaagatca tccttgaaaa aggaaagcta
    32401 acaataacta gcatcatata agagctttta aggatagaac agtagaatag gaatctatgg
    32461 gcaagtcctc tcgtataaat aaaataggag ggaagtagca gcatattttt cataaagtgg
    32521 ctctctttct tcatttttac tttggttgct cctatttgaa aattcgagca cactcaaata
    32581 caataaaaag aaatggcaag ggagaggatt agatgaaaaa agaagaaaaa atcgcgattt
    32641 tacaagagat tatccgaatt aaatcagtga atggaaacga aggtgaagta gcagcctatt
    32701 tgaataagct tttggcaaag catgacataa caggagagat tgtttcttac agagatggta
    32761 gagataattt gattgctcgt taccaaaagg gccaatcagg aaaagtgtta ggcctatctg
    32821 gacatatgga tgtcgtggcg gctggtgatg aatcgtcttg gacctatgca ccctttgcag
    32881 cagaaatcca tgggaaccgt ttgtacggac gtggtgctac agatatgaag agtggcttgg
    32941 cagcaatggt gattgccatg atcgaactga aagagtcagg aaaaccattt aacggaacag
    33001 tcaaattatt agccactgtc ggagaagaag tgggtgaact aggtggggaa cagctcacga
    33061 aagcaggcta tgtagatgac ttagatgcct tgatcatcgg agagccaacc aattacagtt
    33121 tgatgtatac gcacatggga tcaatcaact atacagtcac ctcgcacgga aaagaagcgc
    33181 atagctctat gccagaccaa ggttataatg caatcaatca cctaaatgaa ttcattacga
    33241 aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga gaatcctgtc ctaggaaaaa
    33301 caatccataa tgttacattg atcagtggcg ggaatcaagt caacagcatc ccaagccatg
    33361 cgcagctcca agggaacatt cgatcgattc cagaataccc aaatgataaa atcattgcat
    33421 tgctgcaatc aatcgtcaat gaattgaatc aagagacaga ctatcatttg gaattgatga
    33481 tcgattacaa caaaatacct gtcaaagctg atccagattc tcctttgatt cattgcattc
    33541 agcaacaatt cagccagcca ttacctttag ttggagcggc agcaacaacg gatgcagccg
    33601 aatttacgaa agctaaccat tccttcgact ttgtcgtctt tgggcctggt gtcgttactt
    33661 tgcctcatca agtagatgag tatgttgaaa tcgataatta tttggatatg atcgaaaaat
    33721 atcaaggaat tattttatcc tacttagcgt aagatttttt taat
//
LOCUS       DASOUD010000020        32240 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.020, whole genome shotgun sequence.
ACCESSION   DASOUD010000020 DASOUD010000000
VERSION     DASOUD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.020"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            817..2427
                     /locus_tag="U4A29_000887"
     CDS             817..2427
                     /locus_tag="U4A29_000887"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO0680026.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2719..3588
                     /locus_tag="U4A29_000888"
     CDS             2719..3588
                     /locus_tag="U4A29_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO0680027.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3753..5024
                     /locus_tag="U4A29_000889"
     CDS             3753..5024
                     /locus_tag="U4A29_000889"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO0680028.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            5021..6316
                     /gene="rho"
                     /locus_tag="U4A29_000890"
     CDS             5021..6316
                     /gene="rho"
                     /locus_tag="U4A29_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEO0680029.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6415..6678
                     /locus_tag="U4A29_000891"
     CDS             6415..6678
                     /locus_tag="U4A29_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO0680030.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            7123..7572
                     /locus_tag="U4A29_000892"
     CDS             7123..7572
                     /locus_tag="U4A29_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO0680031.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7629..9047
                     /locus_tag="U4A29_000893"
     CDS             7629..9047
                     /locus_tag="U4A29_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO0680032.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            9047..9907
                     /locus_tag="U4A29_000894"
     CDS             9047..9907
                     /locus_tag="U4A29_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEO0680033.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            10081..10854
                     /locus_tag="U4A29_000895"
     CDS             10081..10854
                     /locus_tag="U4A29_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO0680034.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            11001..11366
                     /locus_tag="U4A29_000896"
     CDS             11001..11366
                     /locus_tag="U4A29_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEO0680035.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11514..12722)
                     /locus_tag="U4A29_000897"
     CDS             complement(11514..12722)
                     /locus_tag="U4A29_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO0680036.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12741..13019)
                     /locus_tag="U4A29_000898"
     CDS             complement(12741..13019)
                     /locus_tag="U4A29_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680037.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13173..13868
                     /locus_tag="U4A29_000899"
     CDS             13173..13868
                     /locus_tag="U4A29_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO0680038.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(14310..15107)
                     /locus_tag="U4A29_000900"
     CDS             complement(14310..15107)
                     /locus_tag="U4A29_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEO0680039.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(15104..16288)
                     /locus_tag="U4A29_000901"
     CDS             complement(15104..16288)
                     /locus_tag="U4A29_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEO0680040.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16313..17560)
                     /locus_tag="U4A29_000902"
     CDS             complement(16313..17560)
                     /locus_tag="U4A29_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO0680041.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17775..19520
                     /gene="dnaX"
                     /locus_tag="U4A29_000903"
     CDS             17775..19520
                     /gene="dnaX"
                     /locus_tag="U4A29_000903"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO0680042.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19540..19854
                     /locus_tag="U4A29_000904"
     CDS             19540..19854
                     /locus_tag="U4A29_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO0680043.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19879..20475
                     /gene="recR"
                     /locus_tag="U4A29_000905"
     CDS             19879..20475
                     /gene="recR"
                     /locus_tag="U4A29_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO0680044.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20539..21183
                     /locus_tag="U4A29_000906"
     CDS             20539..21183
                     /locus_tag="U4A29_000906"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO0680045.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21198..21527
                     /locus_tag="U4A29_000907"
     CDS             21198..21527
                     /locus_tag="U4A29_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEO0680046.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21541..22479
                     /gene="holB"
                     /locus_tag="U4A29_000908"
     CDS             21541..22479
                     /gene="holB"
                     /locus_tag="U4A29_000908"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO0680047.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22515..23339
                     /locus_tag="U4A29_000909"
     CDS             22515..23339
                     /locus_tag="U4A29_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEO0680048.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23332..23679
                     /locus_tag="U4A29_000910"
     CDS             23332..23679
                     /locus_tag="U4A29_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO0680049.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23748..24620
                     /gene="rsmI"
                     /locus_tag="U4A29_000911"
     CDS             23748..24620
                     /gene="rsmI"
                     /locus_tag="U4A29_000911"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO0680050.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24729..25850
                     /gene="dinB"
                     /locus_tag="U4A29_000912"
     CDS             24729..25850
                     /gene="dinB"
                     /locus_tag="U4A29_000912"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO0680051.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25904..26506)
                     /gene="nrdG"
                     /locus_tag="U4A29_000913"
     CDS             complement(25904..26506)
                     /gene="nrdG"
                     /locus_tag="U4A29_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO0680052.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26821..29010)
                     /gene="nrdD"
                     /locus_tag="U4A29_000914"
     CDS             complement(26821..29010)
                     /gene="nrdD"
                     /locus_tag="U4A29_000914"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO0680053.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29309..30103
                     /locus_tag="U4A29_000915"
     CDS             29309..30103
                     /locus_tag="U4A29_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680054.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            30093..32135
                     /locus_tag="U4A29_000916"
     CDS             30093..32135
                     /locus_tag="U4A29_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680055.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        10357 a         5738 c         6742 g         9403 t
ORIGIN      
        1 accgtttatc cagaggttgt gagcctacca tttaactccg aatcacaaga aataatttca
       61 aaaacagctt ctcatgtttg atggatattg tgacttgtga ccgaggagtt ggcaggagaa
      121 caccgtttat ttagaaataa ggaggcacga cttttgtcgc atctccttat tttttactta
      181 tgtgattcgt taccttcaat gaatccctac aaggcacttt tgttgaaaaa aaggtcatct
      241 ttgaaaaaat tagtggaatt gttttgtttt tgatattatt taatagaaaa gatatgccaa
      301 aaatccgaga actgtggact acgtttttta cgtaaaggag gtaaataagg gtgaaagaaa
      361 aagattgttt ctacgataag ctatataaac tgttgctgca agtaggaaaa agtagttctt
      421 tgttaggtgg gatattatct atcataatgt ttgttttatt tttttatcta ttatttcact
      481 taaaattttt cagtaacgat tttctgattt gcttaggttt aacgttagct atgtttatat
      541 caagcttatt atgtgggtta aatttttttt gcactcataa aggacttaag aaataatttc
      601 tcaaaggata tcgaaaaaga aaaaattgta agcggatagt attcatttga gtacttgact
      661 ttcccttagt cagcttgtat tatacacctt gggcaccctt ttagagggtc agaagtttac
      721 agaatttcgg ctccctattc ttattgaaga atagggagct ttgtttattg tctgagactt
      781 ttcctgcaca taaattaaaa aggagttttt gaacttatga caaaatatat ttttgtgacc
      841 ggcggtgtcg tttcatcaat cggaaaaggg attgttgcag cttctttagg acgcttattg
      901 aaaaaccgcg gcttaaaagt aactatccaa aaatttgatc catacatcaa cgtggatccg
      961 gggacgatga gcccatatca gcacggagaa gtattcgtaa cagatgatgg tgctgaaaca
     1021 gatttggatc tgggccacta cgaacgtttt attgacatca atttgaacaa atactccaac
     1081 gtgacgacag gaaaaatcta ttctgaagtt ttgcgtaaag aacgtaaggg cgaatacttg
     1141 ggagcaactg ttcaagttat tcctcatatt acaaatgaga tcaaagaaaa aatcatgcga
     1201 gcagctaaaa tgactgattc agatgtgatc attacagaag tagggggaac agtaggggat
     1261 atagaatctt tgccattcct tgaagcactt cgtcaaatga aagcagatgt cggtacagat
     1321 aatgttatgt atatccatac aacattgatt ccttacttga aagctgctgg agaaatgaaa
     1381 actaaaccaa cgcagcacag tgtaaaagag ctacgtggtt taggtatcca accgaatatt
     1441 ttggtggtac gtacagagca acctgtttct caaagcgtga aaaacaaatt ggcacaattt
     1501 tgtgatgtcg aaccagaagc agttatcgag tcgccagatg tagatacact ttactccatc
     1561 ccattagttt tacaagcaca aggaatggat caaattgttt gcgaccattt aaaactagat
     1621 acaccagaag cagacatgac agaatggaaa gaattagaag aacgtgtctt gaacttgaag
     1681 aaaaaagtac ggattgcgct tgtcggtaaa tatgtcgagt taccagatgc atatatctca
     1741 gtagttgaag cattgaaaca ttctggtttc gcctataatt cagacatcga gatcgattgg
     1801 atcaaagcac aagaattaac gagagaaaat gtagaagaac gtttgaaaga cgcagatgga
     1861 atcttggttc ctggtggatt tggtgatcgc ggagtagaag ggaaaatcga agcgattcgt
     1921 tatgctcgcg aaaatgacgt tccgttttta ggtatttgtc ttggtatgca gatggcttgt
     1981 gtcgaatttg cacgtaacgt cgttggatta gaagatgcgt catcagctga aacgaatcca
     2041 gatacagcaa acaatattat tgatttaatg gctgatcagg aaaacatcga gaatttgggt
     2101 ggaactttac gccttggatt atacccatgt aaaatcaaaa aaggaacgaa gacggctgaa
     2161 gcatatgatg gagccgatgt tgtgcaagaa cgtcaccgtc accgctatga gttcaataac
     2221 aaatatcgtc aattgtttga agaacaaggg ctagtctttt caggtgtctc accagataac
     2281 cgtttagtag aaatcgtaga gctgccagag aaaaaattct ttgtgggatg ccaattccac
     2341 ccagaattga tttctcgtcc aaatcgcccg caaaaattaa tcaaagcatt tgtcggagca
     2401 tcattagcag atcacgaaac aaaataattt ttgaacttga tagaaagaga aaagtagcaa
     2461 gagaagggga taagcaattt tcttgtcact tttctctttt tctaatttgt tattatttgt
     2521 aggaaaaaag atagaaagca aaagactttt ttcgattaaa gcgctattgg atattttatt
     2581 ttttgcaaaa aacttcttga aaattttcat tttcattgat tttagtagaa aactaccgaa
     2641 atgtttggta aaatagggtc gttggttaag aattgtctta acaaatttag ttttacaaaa
     2701 cttaggagga attttattat gccagtagta tcaggagctg aatttttaaa agctgctcgt
     2761 aaaggcggtt acgctgtcgg cggattcaac acaaacaact tggaatggac tcaagcaatc
     2821 cttgaagcgg ctgaagcaaa acaagcacca gtacttatcc aaacatcaat gggtgcagct
     2881 aaatacatgg gtggttacaa aatctgtaaa gatttaatca ctgatttagt tgaagcaatg
     2941 aacatcactg ttccagttgc tatccactta gaccatggtg attacgaagc tgctttggaa
     3001 tgtatcgaat taggctatac ttcagtaatg ttcgatggtt cacatctacc atttgaagaa
     3061 aacttgaaat tagctaaaga cgttgttgaa aaagcacatg caaaaggtat ctcagttgaa
     3121 tgtgaagttg gttcaatcgg cggagaagaa gacggaatca tcggtaacgg cgaattagct
     3181 gatattgaag aatgcaaaca aatggttgct acaggtatcg actacttagc ttgcggtatc
     3241 ggtaacatcc atggtcaata tccagaaaac tggaaaggat tagcttttga ccacttacaa
     3301 gcaattgctg atgctgtagg tccagacatg cctttagtat tacatggcgg atcaggtatt
     3361 cctcaagatc aaatcgaaaa agctatctca atgggtatct caaaagtaaa tgttaacact
     3421 gaattccaat tatcatttgc tgcagctact cgtaaatata tcgaagctgg taaagataaa
     3481 gaaggtaaag gattcgaccc tcgtaaatta ttagctccag gtaaagcagc aatcgttgaa
     3541 gatgctaaat ctcatatcga ctggttcggt tcagctaaca aagcgtaagc atccacataa
     3601 cagatacgat atataggaat ggagaacgac ttttgaagtc gctctccatt cttttttact
     3661 taaaacggca gaccatgctc gtttcttttt tttatctatg ataaaataca agcatatcca
     3721 tgaaaaaaga ttaataaaca aggtggaaac agatgaaaaa aatcgtaatc aaaggaaacc
     3781 gacctttagc tggtgaagta acgatcagcg gtgcaaaaaa tagtgcagtc gcattgattc
     3841 cagctgcaat cttagctgat tccccagtga tcttggaagg tgtgccggac atacaagatg
     3901 ttcattcatt aattgaaatt ttagagatca tgggcgcaac tgtccatttt tccaacaaca
     3961 tactagaaat cgatcctcga gaagtcgtat cgattccgat gccgcacgga aaaatcaata
     4021 gtttgcgtgc ttcttattat tttatgggaa cactgcttgg taaatttggc gaagcggtag
     4081 taggcctgcc aggtggctgt tatttaggcc ctagaccgat tgacctacac gtcaaaggat
     4141 ttgaagctct aggtgctgaa gtgacaaatg aacatggtgc catgtattta cggacagaaa
     4201 ataaaggatt gcgtggcaat cggattttca tggacgtggt ttcagtcgga gcaacgatca
     4261 atgtgatgtt agctgctgta aaagcaaaag gacagacagt aattgaaaac gctgcacgag
     4321 aaccggaaat catagatgtg gcaactttac tgaataatat gggggcaaaa gttcgaggag
     4381 cgggtacaga tgtgatccgt atcgaaggag tcgaaacatt acatggctgt cggcatttca
     4441 tgattcctga ccgcatcgaa gcaggaacct atctagcatt agcagctgca gtaggaaatg
     4501 gtatcaaagt gaaaaatgtg atctttgaac atttggaaag ctttatagca aaattgcagg
     4561 aaattggtgt ccatatgaaa atcagcgaag atgaaatcga agtttacccc tcgaaagaat
     4621 taaaacccgc aaacatcatg acctatcctt atccaggatt tgccacagat ctgcagcagc
     4681 cacttactgc gttactattg atgactacag gcacttcaga aatcatcgat acgatctatg
     4741 cgaaacgtgt gaaccatgtg ccggaactag cacgaatggg cgctgatatt accgtggaag
     4801 gaaatatgat catcgtgaat ggtccaaata aacttcacgg cacagaagtc gtggcttctg
     4861 atcttcgggc aggtgcttgt ttagtgattg ctggactatt ggcagaaggg acaacgacga
     4921 tttacaatgt agattatatc ttacgcggtt atgatcatat tatcgagaaa ctaacagcct
     4981 taggggcatc aattgaaatg atagaggaag tgggagctga atgagcgatt atttgacaat
     5041 ggcagaacta gagaacaaaa cgttaaaaga agtttatagt tatgccaaag aatttaaaat
     5101 tccttattac agtaagatga ataaaaagga attatctcta gctgtgattc gtgcacaagc
     5161 agagaaacaa ggattttatt atatggaagg aatattggat atcgttggtc aagatggtta
     5221 cggtttttta cggccaatca attatggacc aagtgcagaa gatatctata tttctgcctc
     5281 acaaatcaga cgattcagtt tgagaaatgg ggacaaagtg gcaggaaaag cccgtccacc
     5341 taaagaatcg gaacgttatt atggtttgat gcacgtggaa agcgttaatg gaaaagatcc
     5401 ggaagaagca aaagaacgtc cgcattttcc agccttaaca cctctttatc cgcaaagaca
     5461 gcttcaactg gaaacaacac cgcaaagact atctacccga atgatcgata tcttttctcc
     5521 aatcggtttt ggtcagcgtg gattgatcgt cgcaccgcca aaagcaggga aaacttctgt
     5581 attaaaagaa atcgcaaatg gtatcaccga aaactatccg gaagtagaat taatcgttct
     5641 actgattgat gaacgaccgg aagaagtaac ggatctagaa cgaagcgtga aaggcgacgt
     5701 ggtatcttct acatttgatc tgcagccgca aaaccatact cgagtttctg aattagtatt
     5761 ggaacgcgca atgcgtttag tcgaagataa acgagatgtc gttatcttga tggatagtat
     5821 tactagactt gctcgggcct ataacttagt cgttccacca agcggacgta cattgagtgg
     5881 cgggattgac ccagccgcat tgtataaacc taagaaattt ttcggggcag caagaaacat
     5941 cgaagaaggt ggcagtttga cgattttagc gaccgcttta gtcgatacag gaagccgtat
     6001 ggatgacgtg atctatgaag aattcaaagg aactggaaat ctagaattac aactttcacg
     6061 tgaattatct gaacgtcgga tcttcccagc catcgatatc aaaaaatcag gtacccgaaa
     6121 agaagaatta ttgatgccaa gtgaagaatt agaagaaatt tggaaacttc gaaaaaatat
     6181 gactggagat tcattagaat atacggaaca actgatccgc tttttgcgta aaacaaaaaa
     6241 caatcagcaa tttttcaaag aatttcaaga tgtctccttt ggtaaaaaaa atccgaccag
     6301 aaatctgaaa agataattgc aaccttgact gaaacatgtt aagatgttaa tgttgtaaat
     6361 cgacaaatca actctggttc agcaaatgga gcagggcaaa ggagcgaaaa aatcatgaaa
     6421 gaaaacatcc atccagatta ccacccagta gtattcatgg attcaacaac tggtttcaaa
     6481 ttcttgtcag gttcaacaaa aacttcacaa gaaacagttg aatgggaaga cggtaataca
     6541 tacccagtca tccgtgtcga agtaacttct gactcacatc cattctacac tggacgtcaa
     6601 aaattcacac aagcagacgg acgtgtggac cgtttcaaca aaaaatacgg tctcaaagac
     6661 gcaaacgctg cggaataata aaaacgctga taactcaacg tttagcagac tatctgatag
     6721 ggatagtctg tttttttatg ttttttgcca atttaaaact tcgtttgatg ctgaaaaagt
     6781 cgtaggatca taatgcacaa aacaatgaaa aactagtata tagttttaat tatccacttg
     6841 ataaaaacac cttatattaa cgaaatgaag aggggttatt tcgaagtaaa aaaccattta
     6901 cctaaaatta gaagctaagc tttcctttca aggaaggctt tttgttttgt tatagacttg
     6961 cgattgtttg tgagctagat cgttcatttt aatgacttat acacgcggat gatgggtctt
     7021 tttcctctgt tcattcataa tattgaacgg tacagctagc gatgctatac tgacctcagt
     7081 tccaagaaaa cgttaacatt ttttgtgggg gagaggtttt ttatgaggtt tgatcaattg
     7141 atcgcagaag aactgattgt tttcgatcca tcgatagatt caaaaggatc attatttgag
     7201 cgaatagggg atgtgttgaa aaaaacaaat aaggtaagaa atgttcaaaa agtcgttaag
     7261 gcactattca aacgagagga ggagatctcc acaggaatag aagatggatt tgggattccc
     7321 catgcaaaaa gcaaatatgt gacaaaacca gcactgtgtt tttttcatac agccaaatta
     7381 acggactata gcgggataga tgaaatgcca attgaatata tttttgccat cgtagttcct
     7441 gaaaaatcag cagatatgca tctagagatt ctcagcacac tctccagacg attgatgaat
     7501 gcagatttta gaaagggaat aaaagaagcc actacgccag cagatatcat cagacagatt
     7561 agtcaagaat gaaaatagta gagtctttgt ccgagtatcg atagtgaaga aaaaggagga
     7621 aaataatcat gaaaatcgtt ggtgtaactg cttgcccgac tggaattgct catacttata
     7681 tgtcggcaga aaaattagaa gtaacagcaa gaaatctaaa ccatcaagca aaattcgaaa
     7741 cacaaggtgt aaaaacagaa aatcagttat ctgaacaaga aatcaaagag gcagatgcaa
     7801 tcattcttgc agtagataaa gaaatcgaat tggaccgatt tgccgggaaa aaagtaaagc
     7861 gtgtctctac aagccgagca atcaaagaac cgcaagtagt aatagacgaa gcattgagag
     7921 atattggcgt attcgttgtg tctaatgaaa aagaaccagc tgcaaatgag aaaaagccga
     7981 caatctataa tcatttcatg aatggtgtga attacatgct tccatttgtg atcgcaggcg
     8041 gaatcatcat cgcactgagt tttgcctttg ggattacagc ggctgatccc aactcagcag
     8101 actataatgt gttagcagct gcattttcaa ggatcggagg agatacagcg tttgccatga
     8161 tggttcctgc gttaggcgcg gggattgcta catcgattgc tggaaaagca ggttttgccc
     8221 caggaattgt tgccggatta ctggcctcta caggtggttc aggttttctt ggcggaatga
     8281 ttggtggcgt gctagcaggg tatatctgtg attttctggc aaataaaatt agcgtaaaaa
     8341 aagaattttc tgctatttat cagctgattg tcgtcccctt tttatcgatc ttaattattg
     8401 ggcttttgat ggtatttgtg attgaacaac cgatcacatg ggtgttggat gctttgacaa
     8461 attggctgaa tagcttggga aatacatccg ggctattgtt cggattactt atcgggatca
     8521 tgatggcagc tgatatgggt gggccgatca ataaatcgat ttcgactttc tcgatcggtt
     8581 tgatgtcagc tggagtaaat gcaccgattg ctgcttgtat ggctgcagga atgacacctc
     8641 ccctaggtat tgctttagcc actttattat tcaaacataa atttaccaaa gaagaacgga
     8701 cttcaggtca atcatgctgg attttaggcg cttcgtatat tacagaaggt gctataccgt
     8761 ttgcggtatc tgacccgatc cgcgtgatac ctagtttgat gacaggttca gcagtcgctg
     8821 cagggatttc catggcagta ggagttactt caatggcacc tcatggaggg atttgggtga
     8881 tgctaattcc aaatgttatg aacaatttac ctatgtatgt cttggcaatt gcatcaggta
     8941 cagtcactac agcctttatg cttggactat tgaaaaaaac agtgaaagag aaaaaacaag
     9001 acaagtggac gaaaaatgaa aatctaacaa atgaggtgga agcgtaatgt tatatacaat
     9061 gaaagagtta ttgaaagtgg ctaaagagaa tcaatttgca gtaccagcct tcaatatttg
     9121 cagctatgat atgatgaaag caatcatcga agaagcagag agattagaag caccagtaat
     9181 cttagaaatt catcctgatg aaatcgccta tttaagtgat gagtttgttg cggcagtaag
     9241 agcctatgct tatacaagta aactgccgat cgttatccac ttggatcatg gcggtacgat
     9301 agaagatgtc ttacgggcga ttcgaaacgg ctattcttcc gtgatgatcg atgcgtcttt
     9361 gaaatcattc gacgacaatg tacagattac gaaaaaagtg gttgcgttag ctcatgcggt
     9421 aaatgtctca gtggaggctg agcttggaac gattgggaat aatggttctg ctgaaggagg
     9481 tgctgaaaca atcatttata cagatcctga tcaagcgaag atttttgtga aagaaacagg
     9541 aatcgataca cttgctgtag caattggaac agcgcatggc ttgtatccga aaaacagaac
     9601 tccaaaatta aatatcgact tactgaaaga attgaaccaa caaatcgatc ttccatttgt
     9661 tttacatggc ggttcaggta atccggacgc agaagtcagc gaagcagtaa aatacggagt
     9721 tgccaaagtg aatttgagct cagatcttaa aagcgtcttt ttcaatgctt tgagagaaat
     9781 attgagtgaa aatccatcga tgtacgagcc gaatgcaatt tatccagcag ctaataaaaa
     9841 ggtacaagaa gtagtcaaac ataaaatgga aattttgaac acagtaggca aggctagttt
     9901 atactaaaaa taaaaatgtg tggtcaagag ggtatgacaa tagtcttgtc ataccctctt
     9961 aaccacacac gatgttcaga agtagttttt tcgaaaaatt tcttcttatt tctcgaagcc
    10021 aatcacttct gtcacaacct cttttttcct gttactatat aaagtaaatg gggtgatttt
    10081 atgttgaatg aagtggagaa tagacagcag gagattgtga attatctaaa aaacaaccaa
    10141 tttgcaagag tcaccgattt gatcgagtta gtaaattata gcgaagccac gattaaacgt
    10201 gaccttgttt ttctggaaaa aagtggattg atccgtagag ttcgcggtgg cgccatgctt
    10261 attgacaatc gaaaaattga cgtgccttac ctgatgaaga tcaccaaatt agaagaagaa
    10321 acagaaaaac gtaagattgc agatacagct tcttctttga ttcgagatga tatggtctta
    10381 ttcttggatt ccagtacaac ctctttacac ttagtcagaa acctagcaag atttgatggt
    10441 ttgcagatca ttacgaatgg catcgtcaca gctgctatgt tgagtgaata tacaacggca
    10501 aaagtatctg tattgggagg ggcaattgtt ccgaaacgag caacaatcaa cggttcaaaa
    10561 gcctataacg atgcattgtc atattatgcg gatttagcga taatgagttg tcgcgggcta
    10621 gatttcagcc aaggagtaac agaaactcat gaaggagagg caatggtcaa aaaagctttt
    10681 agaaaacaat cagaccggct tgccgtttta gttacacagc aaaaaattgc acataaattc
    10741 atgcatcaag ccattgcttt tcatgaaatc gattatctgg tgacaagtaa agaactgaca
    10801 aaagaacagt atgcgaaagt aaaggaacac catatcgaat gtctgtacgg ataaattatt
    10861 acgtgaatgt tctaagtggc ttcctcattt ttagatagga ccttatcttt gctcgcttct
    10921 ctcagccgat ggtataataa tgaaaaggtt atcttgttag ataatcttac ttttttatcc
    10981 aggagaaagg agtgcagaaa atgaaacatg tgaaggcgtt ggtagtgaag gcaatcatga
    11041 tctgggcgat tctgtgggtc gtgctgacag gtttatacgg tgttagcttt atggattcaa
    11101 cgattgttgg ggtaattatt gtagtcatga tctatgtgct aggggatctg ttgatcttac
    11161 gtaaagtagg gaacatcgca gcgacgatcg cagatgctgg ttcagctgca gttatcttgt
    11221 ggctctatct atattttatg aatgatacag tcgatatttg gatgaaagta ttgattcctg
    11281 ctctattgat cggtgtggca gaatggttct tccacaagtg gttgctaagt caaggcatcg
    11341 taccagatga acggaaaatg gaataaatca acagttaaac tattcttatc gataaatgaa
    11401 agctttaaaa ataaatagat gtttaattaa gaataatcat aaactatttt tactcaaaaa
    11461 accgctcaga ttattttcag atctgaacgg ttttttttat ctattcatca agatcaaatt
    11521 tcatttttca atgattcaat caattcgccc tctgcttcat gactatgttt tcggccatta
    11581 acaagataga gtgctgtaca tatcaatgga atgactccgg caatcacata caatcctcta
    11641 aatgaagtaa gagattttag catccccatc aaataaggac caacgcctaa gcctaggtct
    11701 aaaccaataa agtaggtaga taaagcaata ctgattcgat gttcgcggac gatttttaaa
    11761 caaacagcct gtccatttga cataaacgta ccataaccta ggccgactaa tgctccagag
    11821 ataagcaatg tcacactatt cgtcgttaca ctcagtaaaa ataatcctgc tgttaagaat
    11881 aaatagctag gatacatcac gtattgttct cctttgacat cgaaaatcct tccagtcaat
    11941 ggacgtgtaa atgtgatgac caaagcatat acgacgaaga aaaatgaccc agcagcaact
    12001 aagtggatta cttttgtata agaagataaa aatgataaaa cacttgaata agaaagaccc
    12061 atcaaaaagg caattcctgt aatgaaaagt gctttctttt caataaaact attgatattc
    12121 catgagcgaa gagatatttt ttgttcttca gataatggaa tattttttac aggaaagaca
    12181 aggcaggcaa tcgtaacaaa gaagaccagt acaattgaaa accagataat gaaacgaaaa
    12241 tcagtcatat tcagcaaaat cattcctaaa aatggcccaa cagcggcagc taaactagta
    12301 cttaatccat aataattgat gccttcccct tttttagatg cagggatata tgcagtaacg
    12361 atagcatttg tagctgtaga aactgtaccg tagccgaatc cattcaagaa tcgaactaaa
    12421 tacaatgccc cgatagttgg cgtataaaga tatgctaatg ttgaaaggag atagaagatc
    12481 gcgccgcctc ttaaggtaaa cttccgacca aatagttcta actgtttccc catgaataag
    12541 cgtgccagca atgttccgat gatatagatc ccagaagcaa aacctgcttg gctcaagctg
    12601 gcgtgtaagg tgtcttgcgc aatgactgcc atgatgacca tcaacagata ataaatcagg
    12661 tagacaataa aattgatcaa tgtgattcca ataaaatatt tattaaataa tttcttttcc
    12721 atgtatgtta tacattcccc ttaagctgaa tatgctttta atttttgtat ttgaacttct
    12781 acatcgtgat aattggatgg atagcgatca acaagttggc gaataaataa atctcttgta
    12841 gacataaaca gatttaaaaa ttcttcgcat tcgtttgttg gattctcatc cactgctttg
    12901 atcaattcga tcgagttttg atacgcatca tttaaaaaga ctaaagaagc tttcatatca
    12961 aatcttgttt gatatgctgt ttctaatcga ttaatgattg tgttcatcca gtaattcatg
    13021 ttttttccct ctattcttaa aaaattttac ttctgcagta taacgaataa aaaaaaggaa
    13081 aaaaatcgca taagtgatgg aaaaaggtac ttaacaaatg ttaatatcat tagtaaaata
    13141 cctttggact ttaaaaagtt ttaaagtact gtatgatctt tttagatgaa agtttggatg
    13201 ggggagcatc attggatagt agagtcttaa aggaatattt ggataaaaat gattttccga
    13261 ttatacgcaa aggatatcat aaatatttga cattcgaagg gatggaagat tcccatacgt
    13321 atatcttgaa agagggaacg gtaaaagcaa gtgttatttc aaatgatggg agagaattta
    13381 accttcgcta tatcaaagat cttgaaatcg tctctctttt aaaagacgaa tattcacaat
    13441 tcatcgattc gccgtataat attcgagttg aatcagaata tgcagagttt tatcgaatca
    13501 atcgtgtgcg cttttggcaa aatatcaacc aagacccaaa gctacaacac tatgtaaaag
    13561 agtattaccg ctatcgtctg ctttattcga tgaaaaaaat gcagcaaatg ctattaaacg
    13621 gaaagctggg agctgtttgt acgcaacttt acgaattata tgatttattc ggtgtgcgga
    13681 cagaagaagg gatgctgatc gatttttcga tcacgaatga agaaatcgcc aaattttgtg
    13741 gcatctcaac tgctagcagt gtaaacagaa ttttaaaaca actgaaaaaa gatggtatta
    13801 tcaccgttaa agaccatcat atcgttattt tgaatatgga cttgttagaa gacaatatta
    13861 tcttttgatg aacaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    13921 acggaaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    13981 tgaacaccgt ttaccaagag gttgtgagcc tgccgtttaa ctctgagtca caaggaacaa
    14041 tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga ggagttggta
    14101 ggagaacacc gtttaccaag aggttgtgaa aaagctgttc tgcatcaagt aataagaaga
    14161 gccaagtaaa aacagtctct tatgttttta acttggctgg acttatcacc gtagatgtaa
    14221 gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga cacgattttt gtcacagccc
    14281 tttttttgcg aacattcatt tagcaatttt tagaaacttc tgccgccacc gccaaaagtc
    14341 ccgccaccgc tagaatgggt agtacttccg ccaccgccgc ttccaggagg tgggcttttg
    14401 gggattcttc ttgtcgtgac aaaagaattt gtcagacgat cggttttttc agttaatttg
    14461 atactggatt tttctcggaa tggatatttg tagatgcctg atttcaattg ataacgcgaa
    14521 acagtcacaa aataaaagac aagactaagg ataagagcca aaacaacagc aatgatgatt
    14581 tctactgtcg tcaaaacctt atagcgtgta attttgccag tttcttcgtc tacacgataa
    14641 tgtcctcctg gaactccgtc ttccacatat gttgctgatt tagacaggta agcttttgca
    14701 gcattgtaat aggcagcatt tgtcatctga tcataaacat catctaagat cgaatcgatt
    14761 ctgctatcag tcaaataatc gatcatatta ccagaagtcg aaatgtagat ttcacgctgt
    14821 cccatatcta atagcaatac tgagccattt tgatcatttc cgactgcgtc tcttaagtaa
    14881 ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag tcgttacgat caagactcgt
    14941 cctttgattt tttcattcag cttttctgcc tgttgttcca aagattggat ttgatctgtt
    15001 gtgaataacc cagcctcatc atttaccgta gctgtatctg catgagtagc caccggccag
    15061 ccaacactta ctagcaaaag aaagaataaa aaaaattttt tcatcatatc aagtaacctc
    15121 cgatcagaaa gaggatcgct agcccagcga aaatcccaag agcggtcagt cccaattttt
    15181 tctggctgat cggcaggaca ccgctgacct ttcctgtttg tccattcatt gcgtagtagt
    15241 agactttttt acttgagtca ttgcttcgat aagtaacaag ccataccggc agtaatacgt
    15301 aattatttac ttcgtttgtg attgaagcag aagttcgcac accggtcaat gttgtatatc
    15361 catttgcggt atcttttaac agcttctctg aatagtcttt taattcagat tgaatctgtg
    15421 atttgattgc ctcatattcg atatctcttt tttctgcttg gaacccggct aaatactggc
    15481 ttttaaaatc aattgcttta tctaacggga aaggttgcac gccttcgacc atcttttgtt
    15541 gcgtattttt tgataaggca ttttttacta gttctttaaa agaaagcttg cctgctcttg
    15601 aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc tatccgccat atgcggataa
    15661 ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc tgtagcccaa taagggaaat
    15721 acacacctgt caacttatcg atttgatcct tattgaaaaa agctttggga acaaaccatt
    15781 ttttcttggt ccaagcgaga aatctgttga ttgcttcttc tttctcgaca gcaaaaggaa
    15841 gtactttatt tggcaagaat tctccgctta aacgtcccga tagtacgacg ggattatggc
    15901 agtagtaaca ataagtcgca gcagttgtcg catcagtcac gatctctgca ccacagttgg
    15961 gacaaagaaa aagatctatc gctgcggtat tatctgcaga ttcgtccgct gtatctgaaa
    16021 aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt catatcggca agctgttctt
    16081 gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc tttgctttct tcgccagcca
    16141 tacgcgcttc tttttgcttt tgctcatatt ggctgacttc ttcttctgta tagatattca
    16201 ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa agtcaacgga ccgccgcaat
    16261 taggacattt atgtgtaaga acatccatca gctgcacacc tttctaaata aattaattaa
    16321 cggggattcc tttaaacggt ttaccacagt ttggacagaa tttagggatg ccgttggaaa
    16381 gatccacgat ctcattgcat tcactgcatc gcatttgtag cttaggctga acgttttcta
    16441 caggtttggc agctccgcag ttggaacaga attttccgga attttctgtc ccgcaaactg
    16501 gacatgtcca agtatccgca tttccttgtg cagtttgttg attttgtttt tctgcttgtt
    16561 gtttgatttg ttcttggtta ttttgagctg cttgagaaag ataagaacca tttgcattca
    16621 ttcccatacc tacacccata aagccggtca ttgctcctgc ttcatttttt ccggctgctt
    16681 ccataccacg tgcaatggaa ccttgcacgt atccttcacg tacgctagga tcacctagca
    16741 tcgccccttc gttacgcata ttgatcagtt tcacagaatc atcggtgtaa gaaatgctgg
    16801 ctacagccac agaaacaatc tccatcccac gtaattcacg ccaagaatca tccaatgcag
    16861 tatccatgta tttgcttaat tccaaactct tagatggtac ataagaaatc cgttgaccat
    16921 ctgcagacat ctgattgatt gctgattgta gcgctgttag gaattcagct aaatactgct
    16981 cattgatatc attgatttcc acttgtgttt tgtttttagg gattgcgttt gtataaaata
    17041 aaatcgggtc aacgacatga atagaataag tgccatgtgc ccgtaagaat aattctgcgt
    17101 tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc gaatttgatt ccctttattt
    17161 cttggagatt gatataaaaa acttgttgtt tctgcggtgt gacgccgcca aatttgaatc
    17221 ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt gaacatggaa ggagctgcgt
    17281 catttttaat ggtgtaataa ccttcctcag cggtataatc gatgattttt ccaccatcga
    17341 ccaaaagcat catcatgttt ggatagacat ggacgactgt tccgtctgtt aaaacatctt
    17401 ctgttccttt acgattggag ccacgtttgt catctttgcg gactttgaca ccttttgtca
    17461 tgaccgttgt atcactcata ttgtctggtt cgatgacttc tagccattga tctgccagac
    17521 cgccgccaac catacttgtt gctgctttga taagacccat aaaaaattcc tcctctgatt
    17581 cagctagtgc caaacaatta ttgtttattt attataccat aggaagatgt gaaacttctc
    17641 ggactagaga cggaattgaa atggaaaatt gcgtattgtt taaatacctg aaaaagccga
    17701 gaaggatagt agttttttta cctaatatca cgtataataa gagatagtga attttttaaa
    17761 aaagggggat ccttatggca tatcaagcac tttatcgagt ctggcgttcg cagcgtttcg
    17821 aagacatcgt tggacaaaaa gctgtgacac agacactaaa aaatgctatt gtttcccaca
    17881 aaacctcaca tgcctacttg tttacaggac ccagaggaac cggaaagacg agtgcagcaa
    17941 agattttcgc aaaagcaatc aactgtccga acagtaaaga cggtgagcca tgcaacgaat
    18001 gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga tgtgatcgaa atcgatgctg
    18061 ccagcaataa cggagtcgaa gaaatccgat ttatccgtga tcgggcaaat tatgcgccta
    18121 caaaggcagc gtacaaagtg tacattattg atgaggtaca tatgctttca acaggagctt
    18181 tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag tgtcatattc atcttagcaa
    18241 cgaccgaacc tcacaaaata ccagctacga tcatttcacg gactcagcga tttgatttta
    18301 aacggatcaa tacggcagat attgtcgaac atcttgaatt tatcttgaaa gaatcgacta
    18361 tcagctatga accagctgca ttgaatgtga ttgcccgttc tgcagaaggc gggatgcggg
    18421 atgcattgag tattcttgat caggcaatct cgtttagtga aggaacaatt actttagatg
    18481 atgccatgca agtgacaggt agcttaactg acgaaatgat ggacagttac ttgagtgctt
    18541 gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa ccaaattctc gcagccggca
    18601 aagaatcacg tcgctttcta gaggatatgt tacaatactg ccgtgacttg ctgatgtatc
    18661 agcaagcacc taacctagtt gaggaacaat cgagtacgat cagaagtaca tttacagaat
    18721 tggcacaaac tgtttcagcg gatcagctgt tttcaatgat aaagattttg agcgatactc
    18781 aaaatgaaat tcgttttacc aatagtgccg ctatttattt ggaagtcgct acagtcaaac
    18841 ttgcacagct accgtctgct tctgttcctt cagcagatgt agcatttgca gatgatcaga
    18901 tagtaaaaca gctacaacaa gagattaagg aactaagaaa cgaaatcaag gaactacgca
    18961 gtaatacagg aattgagaat acaaaagtac agccggtaca gcaaacaaat cataaaaaac
    19021 agacaagtac ctatcgtgta ccgacggaac gagtatataa agttctgcgg gaagcgacaa
    19081 gacagcatct gaaccaagta aaagaagtat gggaagactt gctgatgagt ctatctgtca
    19141 ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc tggacctaca ggactagtga
    19201 ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa tgacgaagaa ttgcagcttt
    19261 cggtacacaa caacttaagc cgaatgatca aagattatgc gcctgatcct gtttatatta
    19321 caagagaaag ctggccagaa cttcgaagaa attatttgac gcaggcacaa gaagagggaa
    19381 cagaaaactc ttttgcttct gaagactcag aaggagaaat cgagttgctt cctcaagaac
    19441 aagaagtacc agaagaaaca cttgtagtaa aagcagagga actgtttggc tccgaagctg
    19501 ttacagtggt agatgattaa taaaaaggag tagataatta tgatgcgtgg aatgggcaat
    19561 atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag aaatggcaga agcacaagag
    19621 ctgctaaata aaacagagtt tacaggtaaa gccaccaatg aattggtcaa agcggttttt
    19681 acaggcgatc gtcgcatgaa agatatccaa atcaatgaag aagtcgttga tcctgatgat
    19741 atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg atgcgatcgc acaaattgat
    19801 gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt tgccaggatt ttagttttaa
    19861 actgaaggag taatgaatat gcaatatcca gaaccaattg cgaaactgat cgacagctac
    19921 atgaaacttc caggaatcgg tcagaaaaca gcgacaagac tcgcctttta tacgatcgat
    19981 atgaaagatg aagtagtaaa tgaatttgct aaatctttgt taagtgtaaa acgcgatttg
    20041 catttttgta gtatctgcgg aaacatcaca gaagaagatc cctgcgaaat atgtagaaat
    20101 ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc cgaaagatgt aatggcaatg
    20161 gaaaaaatgc gagaatatca cggactttat catgttctcc atggtgtgtt atcaccgatg
    20221 gaagggacag gtccagaaga catcaatatt tcttctctgt tgcagcggct gcatgatgca
    20281 gatgtaaaag aagtgattat cgcaacaaat gcaacaacag agggagaagc aacagctatg
    20341 tatctctcac gtctgatcaa acccgcaggt atcaaagtga ctcgtctagc acatggtctg
    20401 tctgtaggct ctgatatcga gtatgcagat gaagttactt tattgaaagc tgtcgaagga
    20461 agacgtgagt tataaatttc taattaagtg ttttttcaga tacaatataa aagcagcaca
    20521 agagatgggg gcaacaatgt gaacggacta tttattacaa tcgaggggcc tgatggtgca
    20581 ggaaaaacca gcatcttgaa tgaactttac cctcttttga aagaagtagc aaaaacagaa
    20641 atcattcaaa caagagaacc aggaggcatt ccgattgctg aagagatccg agcagtgatc
    20701 cttgatccaa aaaatgatcg gatggatgaa cgtacggaag cactgcttta tgcagcagca
    20761 agaagacaac atctagtcga aaaggttctg cctgctctgg caaaagggaa aatcgtttta
    20821 tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg caggccgacg catcggcgtt
    20881 accgagattg ctcggttgaa tgagtttgct acagaaggta ctacgccgga ttttaccctt
    20941 tatcttgacg tagattcaga caccggatta agaagaatca agaaaaacag acaaaatcaa
    21001 atcgaccgtt tagattcgga aggtttggaa ttccaccagc gtgtacgtca tgcttactta
    21061 aagctggcag aagagaatcc ggaaagaatc cacaaagttg atgcaagaaa aagctttgaa
    21121 gaagtgctgc aaacaagtta tcatacaatt attgaacagt atccgcaatt ttttgaaaac
    21181 taggaaggtg aacgtttatg aaaattattt tagcgattat ccaagacaaa gacagcaacc
    21241 gtttatcaaa tgaattgatt gatgcaaata tccgtgccac aaaactatca tcgacaggcg
    21301 gcttccttaa ggctggaaac agtacgttta tcgtgggtat tgaagatgac cgtgtagaag
    21361 aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa gcaatttgta tctacacctg
    21421 ttacactgga tatttcgatg gatgggggcg tcccttatcc agtagaagta gaagtaggcg
    21481 gtgccacagt cttcgtctta cctgttgaag gattccatca attttaggag gagctgagct
    21541 atgaatcaag aaataaactt ggctcaaatg caaccgatcg tgtatcagca gcttcaacgg
    21601 agttttgagc atgggcgtat cgctcatgct tatctttttg aaggggagaa aggaacagga
    21661 aaacatgaag taggtatatg gcttgcacag cacctgttct gtacccaaat gaaagacaat
    21721 ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa agaaagaaca tccagatgtt
    21781 ttggttattg aaccggaagg acaaaccatc aaagtcgatc agatcagacg tttgcagacg
    21841 gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt ttttgatcaa agaagctgaa
    21901 aaaatgaata gcagtgcggc aaacagtttg ttgaaattct tggaagagcc ccctggagat
    21961 tttttagcgg tgttagaaac agatgcagta ggaagaattc tccctactat tcaatctcgt
    22021 tgtcaagttc tgcattttca agaactttcg aaagaagcac tgatccatcg attgcaagaa
    22081 gagcagattc cattggaaaa agcaaaactt ttggctttct taacaaacag tttaggaaaa
    22141 gcagttgaaa tctctcaaga tgaatggttt aatgatgcta aggacttgat ttatcaatgg
    22201 ttcgtttact tgcaaaagca agatactcaa gcctttatct atgtccaaaa aaaattggta
    22261 aaatcattta aagaaaaaac acaacagttt acaggattat ctgtcttgat gttttattat
    22321 caggaagcac agcgtcaagc agtagcaaac ggcgaatatg gcaaagtgaa acagataaac
    22381 cagataattg aacggatatt gatcgcagag cagaaactac gcagcaatgt cagttttcag
    22441 gctgttgcgg aacagttcgt tttgcatacg attttttagc ttgccgcaaa ggatgatgat
    22501 caaggaggat ggaaatggta gaagtagtag gtgtccgata tagagatgca ggtcatatct
    22561 attattttgc accaggagac tctgaatatg tttatagtga caaggtatta gtcgaatctc
    22621 agcaatcctt acatattgct actgttgtcc ttcctaaaaa agagattgat cccaatgacc
    22681 ttcctgaagc tatccgtccg atcttgcata aagcgtcaca agctgaatta gaaaaagaca
    22741 aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc aaaagaaaaa gtgcagttgc
    22801 gtgagttgga aatgaaaatg gtcaaagccg aatacacatt tgatcgaaat aaattgattt
    22861 tttctttcac agcggacggg cgaattgact ttagagaatt ggtaaaagat ctggccgctg
    22921 tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg cgatgaagca aagataatag
    22981 gcggaattgg tccatgtggg cgtccattat gctgttcttc ttttttaggc gatttcgtgc
    23041 ctgtatcaat caaaatggca aaagatcaaa gtttatcttt gaatccaaca aaaatttccg
    23101 gtttatgcgg acgcttgatg tgctgtctgc aatatgagag cgaagaatac gagacagcga
    23161 aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc tgatggtaaa gggaaagtcg
    23221 tagggttgaa tctgcttagc cgtatcgtta gtattcggct gactggacgg gaaaatcctg
    23281 tcgaatatga atgggatgaa ttgaaagaat ttatccaagt aggtgagatg aatggataag
    23341 agatcattat acgacggatt gaatcaatta gaatcagagt tgagaaatac attaacagaa
    23401 cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa aaaatacaac tttggagatg
    23461 gaaaatcaac gtctgcggga acacctgcgg gaattgaacc aagttgcaca agctccagct
    23521 gaaaatgtga agcaggaact ttctaagtct cgtatgaatc ttgaaaaaat ttatgaagaa
    23581 ggattccatg tctgctacga tttatatgga tcaagaaggg acaatgacga gccttgtgcg
    23641 ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac caataacaag aggagactgg
    23701 agcggagaaa tactgctcca gtctttttat taaggagatt gtgacagatg tataatcaaa
    23761 aaagtttcaa gggaaatccg gtatatgggg atttatatct tgtaccgacg ccaattggaa
    23821 atttagaaga tatgagctac agaagtgttc gtatgctgca agaatccgat cttatcgcaa
    23881 gtgaggatac gcgtaataca caaaagttat tgaatcattt tgaaatccat acaccccaaa
    23941 aaagtttaca tgaacataat tataaagaac gtgtccctca attgattgct ttgttaaaag
    24001 aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc ttccatcagc gatcctggac
    24061 atgagctagt tttagcctgc ataaaagaag ggatttctgt gatctctatc ccaggaccta
    24121 cagctgggat gacggctttg attgcttcag ggctagtgcc tcaaccattt ttattttatg
    24181 gcttccttcc acgtaagaaa aaagaacaga aagaaacatt gaaaaagcta aaagaacaga
    24241 cagttacgct gattttttat gagtcacctt atcggatcac tgctacattg acgaatttct
    24301 tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga attgacaaag gtccatgaag
    24361 aataccttag aggaagtacg gaagagctac tcgcttatat cgaagaacat ccagtaaagg
    24421 gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat gccagtgacg gaagttgatg
    24481 aagagttggg aacgttaaag gaacaagtag aagcaaagat cgcttcaggc gaaaaaccaa
    24541 atgctgctat caaagcagta gcgctgaaaa atgggctgaa aaaacaagaa gtctaccgtc
    24601 agtaccatga attagactga gatagtataa gttgtctata atgaaagact aatgaggaaa
    24661 tcagctacta acttttcaaa tctcttgaac gcgaacgaat gttcttgtat aatgaataag
    24721 aggtgaggat gatggagctg caatttcctt tacataatga tacatcaaga aaaatcattc
    24781 atattgatat ggatgctttt ttcgcttcgg ttgaagaacg agatcatcct gagtttcgcg
    24841 gacatccatt agtaattgcc agacacccca gtgacactgg tgggcgtgga gtagtgacta
    24901 cagcaaatta tgaagcaaga aaattcggaa ttcattcagc aatgagtgca caaaaagcgt
    24961 atgagctttg tccgcaagcc atctttaagc ctggcgacca tcataaatac agcgatattt
    25021 ccaaacaagt acgggaagta ttctatcgtt atacagatat tgtggaacca gttagtatcg
    25081 atgaagcgta tcttgatgtg accgaaaata aaatcaattg caagtcagcc atcaaaatcg
    25141 cccgaatgat ccaagcagat atttgggaga gtgtccagct aacatgttct gcgggagtaa
    25201 gttacaataa gtttttggcc aaattagcat cagactatca aaaacctaga ggaatcacgg
    25261 tagttgcccc tcaagacgct gtgccatttt taaaagcttt gccaattgaa aaatttcatg
    25321 gggtagggaa gaaaactgta cctagaatgc atgagctagg gatttataca ggaaaagacc
    25381 tatatgaatg cacagaaatg atgttgattc gaaattttgg aaaaatgggc tattctcttt
    25441 atcgtaaagt cagaggaatc catgattctc cagtaaacgt tacaagagag cgaaaatctg
    25501 tgggaaaaga gcatacttat gggcagccgt tgcaaacgga agaagcagtg ctgactcagt
    25561 tgcgccaact agctgaaaaa gtagaagaag cactgagaag agtccaaaaa catgggaaaa
    25621 cagtagtttt gaaggtcagg tacacggact attctactgt gaccaaaaga gtgacattgc
    25681 cagaatatat ctataaaaaa gaagcactct tttatcaagc aagccttata tgggaggaaa
    25741 tcttgggagt agaaaaagga atccgattgt tagggattac tttgacgaat ctggacccga
    25801 tgacttatga aaacatcgtc cttccattat gggaaaatca agaaatataa aaacagcttt
    25861 gagagttgaa accggaaatc cgttttctct cccaaagctg tttttatatc aagtcttgct
    25921 ttttgatttg ttcataggtt tctgttgcat ccgtacattt ttcccaaatc acaacttgat
    25981 tggattctaa agatttttgg acgtcaatga tccgctggtt gctacttccg cgaaattgta
    26041 gttttaggtc acgtttgctt aattcaaagc gtccgtcaac aaggatatcg atttgcgaaa
    26101 ggagttctaa tttatcttct gaatcttgta gcaattcatc aaaggtatag cctgtccatg
    26161 accagatatc ttttgtcgta ccgaactctt ttcggatacg tcgtacgact tgcagacaaa
    26221 catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa tccttgcacg taatcgttgc
    26281 gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa tggtttgcca tatcggaaat
    26341 tttgtgcagc tttgttgaag cagccttcgc aggcaaataa gcagccacta acgtaaagac
    26401 tgttgcggac accttcacca tcgacaaaat taaacggttt gtagtcagca atatagtttt
    26461 ggctgtagtc ttttgctaac cattcttttg gttttggatt tctcataaaa aaacttcttt
    26521 catttaaggt tcaaagatat agctattgaa gataaaatct actcgaccac ataaataaga
    26581 acaaactgac ctaaatccat tgttcaagaa agcttgttct tattttgata gagggcgttc
    26641 aaagatacaa agccatcgaa agagggtggt gaaaaaagac ttgtcatagc ctcttattct
    26701 gaataaacgg tgttcaaaag cttgcatctg cagtaataag cccaactaaa cgaaaaatat
    26761 gaggaactat ttttgtttag ctattcttat tacttgatgc aggacagctt tttcacaacc
    26821 tcatttcatg tgtttcacac gtgaggaaat ttctttatgg cggccatgta ccatcggacg
    26881 agcttgcgga tttcctaaat agccacaagt acgtttgact acatcacatg tttttggatc
    26941 atgatttcca cattctggac attcgaaccc tcgttctgtc ggtttgaaat caccttcgaa
    27001 accacattga tagcaatgat cgattggcgt attcgtacca agatacccga ttttgtcata
    27061 tgcaaaatcc catacagctt ctaaagcttt cggattttgt tggagcatag ggtattcaca
    27121 gtagtggatg aatccacctg aacaatattt tggataatcc ttctcaaatt ccaatttttc
    27181 aaatggcgtt gggtttttac gcacatcata gtggaaactg tttgtataat attctttatc
    27241 tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa cggcagaaac ggtcagttag
    27301 actttcactt ggagttgaat agacactgaa atgataacca tattcatccc cccatgcatc
    27361 tgcattagct tttaattctt tgacgatatc aagtgtgaaa gcttttgctt ctgggtttgt
    27421 ttcccattcg ccaccgaaga aagctgaagc aacttcatac aatccaatgt agcctaacga
    27481 tacagtagct cgacggtttt tgaataattc attgacagaa tcggtacgtg aaagacgttt
    27541 cccgaaagca ccatacatat ataaaattgg cgcatttgct ggaatggctt ctttacaacg
    27601 ttctacacga tagagaagcg catctttcat gatttctaag cggtcagaca gtaactgcca
    27661 gaatttattc tgatctcctt ttgattcgat cgcgattctt ggcaaattca atgtaacaac
    27721 acctaaattc attcgtccga catttacttc ttgaccattt tcgtctttcc acccttgcaa
    27781 gaatgaacga cagcccatag gtactttgaa acttccagtt aattcaacga ttttatcgta
    27841 attcagaata tcaggataca ttcttttcgt tgcacattcc aatgctagtt gtttgatgtc
    27901 gtaattagga tcttcagggt ttaagttcac accttttttc aaagtgaaga tcagtttagg
    27961 gaagatagct gtccgctttt cactccctaa tccattgatt cgaatctgta aaattgcttt
    28021 ttgaatttct ctttcaaacc agttttctcc tagtccaaat cctaatgaag tgaaaggagt
    28081 ttgtccattt gaagtaaata aggtattgat ttcatattcc aaactttgca tagcatcgaa
    28141 gatatctttt ttggtttttg cttttgcata ttctttttgg cgttctggcg aatcgatcca
    28201 ttcttccgcg tcttttagat gttttttata gttcaattct gcaaaaggag caagcaattc
    28261 atctgttcgg tcggcagaac agccaccata ttgacttgac gctacattgg caataatctg
    28321 tgagatttgt gctgtggcgg tctggataga ttttggcgat tcgacttcgg cattgccgat
    28381 tttgaaccca ttgttcagca tgcctttgaa atcaatcaag cagcaattcg tcatcggtgt
    28441 ataaggatga taatccaaat catggtaatg gatatcgcct ttttgatgcg catttgctat
    28501 atgtggtggc aacattttca gtccgattga ttttccaacg atcccggctg tcagatctcg
    28561 ctgtgtattg aaaacattac tgtctttatt tgcgttttcg ttcacgacag tttggtcctt
    28621 gttgattagc ttttcaatcg aaaagttgat atctgttgct ttactgcgag caaaatctcg
    28681 tttggtacga taattgatgt actcttctgc taattcatat tcgttttttt ctagtaatat
    28741 atgttccacg atgttttgaa tctcatagat tttgacattt tcagcaaacc ggcttttgat
    28801 ttcttggaca accaagtctg caatcatttg aattttttca taagtaagtg gtgataatga
    28861 gccgtgaatt ttcttttctg ctttgatcag tgcttcattg atcttttgtt catcgaaatc
    28921 aacatggcgg ccatcgcgtt tgatgacttt catctgggat gtctctggct gctgagtaac
    28981 gacagttttt tcttcataat ttactttcat tatcttcaac tcctgctaaa ttgttcttgt
    29041 ctatcataaa acaaaatcgg ggaaggagca actaaatatc ggctatatat agtattcaat
    29101 gtgctgtata attggatttc tatactatat gtagtgtata ttttattgtg aaaaaaggtg
    29161 aaaacttgta gctggtggat ttgttttttg catatttaat tttgctgaat aaaataattg
    29221 aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa cataagagca aattattcgt
    29281 agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa agaggaggag aagaattgga
    29341 aaagatgatc gaagtgaaac atctaagtaa aagctacggc agcaaacttt ctcccgtcaa
    29401 agccttagat aatatctcat tcacagtaga agaaggcgag tttgttggga ttatgggccc
    29461 aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc acgatcaatt tgcctacgat
    29521 gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg aaaaacagtg agctcagtga
    29581 ctttcgtcga aaaaaactag gattcatttt tcaagagttc aatttgatcg ataccctaaa
    29641 tgcaaaagac aatatcctat tgcccctagc agttgaacgg atgactaaag aggaaatgga
    29701 aaagagagta agacatgtag ctcaattgtt gaatatagaa gaactgctca aacggtatcc
    29761 cgatgaactt tctgtcggac agagacaacg aattgcagct gctagagcac ttgttgttca
    29821 gccacaagtg atttttgcag atgaaccaac tggctctttg gactcgaagt cggctacaga
    29881 attgttgaac tacttgaaga ccatgaatca aaaagaaaaa gcaacgatac tgttagtcac
    29941 ccatgatcca tatacagcaa gctactgcga tagaatttta tttattaaag atggtgtgat
    30001 tttttctgaa gttgtcagaa gagggacaag aagagagttc tttgagaaag taatagacat
    30061 gcaggcgacg attggcggag gaggaaaaat gaatgctgta taaactagcc gttaaaagtt
    30121 tttttcggca gtcacgaggc tatcttgttt attttttcag tttgacttta tccgtaatga
    30181 tctattattc cttcagcgca atgacctacg atcaatcttt agtcagaaga gcaagtcaag
    30241 atacttcgat agatggtgga ttgagttttg gtggatttat cattactgtt gttttgcttt
    30301 ttttcgtatt cagtgccaac cgatttttct tgaatagaag gcaaaaggaa ataggcatct
    30361 atcagctttt tggtatgaat aaactgcaga tatcaggtat atatgtgtta gaaatcatga
    30421 taattggatt gttcgcctgt atttctggga tattactcgg tattattttt tccaaactct
    30481 tttcaatgat tttggttcga atgatggata tggatcttac tagtccattt tttatctcca
    30541 ttccatcagt agtagatact ttatttgctt ttttcatcat ccttttagca gtatctgtat
    30601 attcaatatg gaaaatatgg cgctatccaa tgattcgaag ttttggagaa aaagaacaag
    30661 cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt actcgcagtc atcgggttac
    30721 tacttttaac gagcggatat tttggagcta gtcattttag ggaaatcact tcatggatga
    30781 tcagttatca ttatcttgat acattagttt tcttttttcc ttttttaatt tttttcgttt
    30841 gtgtggtcgg tacttatttg tttttctgtt atacatttcg gttatttttg atcatattga
    30901 gtaaatggaa gatttattac tatggtatca attttttgat aatgggtaac acgcaagttc
    30961 atttgctcaa aagttggcgt acaaattctc tgattacagt cattctcgga atttctttaa
    31021 tgacaattgg cggcacggct agtatctcta ctattttatc ctaccaggag aaagtttcct
    31081 ccccgatgga ctatcagttt gatgttgaga cagagaaaaa aatcaagccg attctagcag
    31141 aggaagggca gaagataact aaagaaatca ccttgcatta taaagtcatt gggagtatct
    31201 atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa tctgatcacg gaaacacaat
    31261 atcaagcctt cagaaaagtt aatccagatt tgccgaacat ttctttgaaa tcacctggtc
    31321 atactgtttt gcttgatcaa atgcaaactc tttttcggaa tatttcatta tatggatcta
    31381 gtattggact gccagataat caagaattga agattcaaca aatgcttcca agtgtattag
    31441 gtgacgaaac aatggtatat ctaagtcctg tactgattgt agatgatacc gtttttgaaa
    31501 aagcaaaagg tgtggattat caagtagtca atgtagatgt atcaggtgga aataatgaaa
    31561 aaatagatga acgactgagc aatgacctgt cgataaagtg gctcaatgct gtttattatt
    31621 cttatgatat cgtaaatgga agtctacaag gagaaattag cacaaagaaa cttccggatg
    31681 accaaatgaa ggaagcacaa tttagccaag aaagctctcg tttgaatttc tcttccagct
    31741 attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa tatttttgtc acactatttg
    31801 tcgggatgat cgtgattatt gctactggta gtattttgac agtccggcaa ttagcagaag
    31861 cagaggaaga aaaagaaaat tatcacctat tgactaagct ggggattcca caaagaagga
    31921 tcagacggat gatcttcgag caaaacgcac ttaccttctt tccacccatg atattaggga
    31981 tcactcacgc agtatttgca atcaatgtct tcactcagta tataaaaact gccgattact
    32041 ggttggctta tcttgtgagt ggcttattga tccttatcta tcttttgctt tatgtcatta
    32101 cgagtcagtt atattgtcgg attatcgagg aatgatatac ggaataagac aagataataa
    32161 atagaactaa attttgaata ccatttattt ggaataagag gttgtgacac gatttttgtc
    32221 acaacctctt tattttttat
//
LOCUS       DASOUD010000021        31382 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.021, whole genome shotgun sequence.
ACCESSION   DASOUD010000021 DASOUD010000000
VERSION     DASOUD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.021"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            141..1010
                     /locus_tag="U4A29_000917"
     CDS             141..1010
                     /locus_tag="U4A29_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEO0680056.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1219..2244
                     /locus_tag="U4A29_000918"
     CDS             1219..2244
                     /locus_tag="U4A29_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO0680057.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2283..3233
                     /gene="manA"
                     /locus_tag="U4A29_000919"
     CDS             2283..3233
                     /gene="manA"
                     /locus_tag="U4A29_000919"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO0680058.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3327..5030
                     /locus_tag="U4A29_000920"
     CDS             3327..5030
                     /locus_tag="U4A29_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO0680059.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5032..7065
                     /locus_tag="U4A29_000921"
     CDS             5032..7065
                     /locus_tag="U4A29_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO0680060.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7076..7516
                     /locus_tag="U4A29_000922"
     CDS             7076..7516
                     /locus_tag="U4A29_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO0680061.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7716..8843)
                     /locus_tag="U4A29_000923"
     CDS             complement(7716..8843)
                     /locus_tag="U4A29_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680062.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8846..9523)
                     /locus_tag="U4A29_000924"
     CDS             complement(8846..9523)
                     /locus_tag="U4A29_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680063.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9787..11553
                     /locus_tag="U4A29_000925"
     CDS             9787..11553
                     /locus_tag="U4A29_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEO0680064.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11568..12005
                     /locus_tag="U4A29_000926"
     CDS             11568..12005
                     /locus_tag="U4A29_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO0680065.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12018..13169
                     /locus_tag="U4A29_000927"
     CDS             12018..13169
                     /locus_tag="U4A29_000927"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEO0680066.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13231..13929)
                     /locus_tag="U4A29_000928"
     CDS             complement(13231..13929)
                     /locus_tag="U4A29_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO0680067.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13922..15274)
                     /locus_tag="U4A29_000929"
     CDS             complement(13922..15274)
                     /locus_tag="U4A29_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO0680068.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15497..16897)
                     /locus_tag="U4A29_000930"
     CDS             complement(15497..16897)
                     /locus_tag="U4A29_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO0680069.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17058..17795
                     /locus_tag="U4A29_000931"
     CDS             17058..17795
                     /locus_tag="U4A29_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO0680070.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17920..18399
                     /locus_tag="U4A29_000932"
     CDS             17920..18399
                     /locus_tag="U4A29_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEO0680071.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18396..19163
                     /locus_tag="U4A29_000933"
     CDS             18396..19163
                     /locus_tag="U4A29_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680072.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19393..19962
                     /gene="lepB"
                     /locus_tag="U4A29_000934"
     CDS             19393..19962
                     /gene="lepB"
                     /locus_tag="U4A29_000934"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO0680073.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20066..20668)
                     /locus_tag="U4A29_000935"
     CDS             complement(20066..20668)
                     /locus_tag="U4A29_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO0680074.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20774..21243
                     /locus_tag="U4A29_000936"
                     /pseudo
     CDS             20774..21243
                     /locus_tag="U4A29_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21349..21933
                     /locus_tag="U4A29_000937"
     CDS             21349..21933
                     /locus_tag="U4A29_000937"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO0680075.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21936..23009
                     /gene="prfA"
                     /locus_tag="U4A29_000938"
     CDS             21936..23009
                     /gene="prfA"
                     /locus_tag="U4A29_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO0680076.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23002..23841
                     /gene="prmC"
                     /locus_tag="U4A29_000939"
     CDS             23002..23841
                     /gene="prmC"
                     /locus_tag="U4A29_000939"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO0680077.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23834..24013
                     /locus_tag="U4A29_000940"
     CDS             23834..24013
                     /locus_tag="U4A29_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680078.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24035..25060
                     /locus_tag="U4A29_000941"
     CDS             24035..25060
                     /locus_tag="U4A29_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO0680079.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25235..26479
                     /locus_tag="U4A29_000942"
     CDS             25235..26479
                     /locus_tag="U4A29_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO0680080.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26632..27579
                     /locus_tag="U4A29_000943"
     CDS             26632..27579
                     /locus_tag="U4A29_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO0680081.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27713..28342
                     /gene="upp"
                     /locus_tag="U4A29_000944"
     CDS             27713..28342
                     /gene="upp"
                     /locus_tag="U4A29_000944"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO0680082.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28481..28723)
                     /locus_tag="U4A29_000945"
     CDS             complement(28481..28723)
                     /locus_tag="U4A29_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680083.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28844..28916
                     /locus_tag="U4A29_000946"
     tRNA            28844..28916
                     /locus_tag="U4A29_000946"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28877..28879,aa:Thr,seq:cgt)
     gene            complement(29035..29673)
                     /locus_tag="U4A29_000947"
     CDS             complement(29035..29673)
                     /locus_tag="U4A29_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO0680084.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29796..30428)
                     /locus_tag="U4A29_000948"
     CDS             complement(29796..30428)
                     /locus_tag="U4A29_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEO0680085.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30779..31078
                     /locus_tag="U4A29_000949"
     CDS             30779..31078
                     /locus_tag="U4A29_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO0680086.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10728 a         5460 c         6310 g         8884 t
ORIGIN      
        1 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
       61 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      121 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      181 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      241 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      301 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      361 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      421 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      481 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      541 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      601 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      661 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      721 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      781 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      841 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
      901 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
      961 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1021 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1081 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1141 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1201 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1261 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1321 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1381 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1441 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1501 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1561 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1621 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1681 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1741 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1801 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1861 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     1921 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     1981 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2041 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2101 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2161 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2221 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2281 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2341 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2401 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2461 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2521 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2581 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2641 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2701 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2761 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2821 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     2881 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     2941 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3001 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3061 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3121 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3181 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
     3241 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3301 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3361 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
     3421 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3481 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3541 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
     3601 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3661 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
     3721 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3781 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3841 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     3901 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     3961 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4021 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4081 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4141 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4201 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4261 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4321 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4381 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4441 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4501 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4561 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4621 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4681 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4741 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4801 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4861 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     4921 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     4981 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5041 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5101 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5161 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5221 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5281 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5341 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5401 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5461 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5521 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5581 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5641 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5701 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
     5761 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5821 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     5881 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     5941 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6001 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6061 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6121 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6181 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6241 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6301 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6361 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6421 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6481 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6541 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
     6601 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6661 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6721 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6781 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6841 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     6901 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     6961 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7021 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7081 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7141 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7201 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7261 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7321 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7381 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7441 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
     7501 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7561 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7621 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7681 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7741 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7801 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7861 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     7921 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     7981 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8041 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
     8101 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8161 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8221 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
     8281 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8341 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
     8401 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8461 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8521 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8581 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8641 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8701 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8761 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8821 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     8881 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     8941 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9001 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9061 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9121 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9181 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9241 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9301 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9361 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9421 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9481 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9541 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9601 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9661 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
     9721 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9781 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9841 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
     9901 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
     9961 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10021 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10081 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10141 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10201 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10261 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10321 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10381 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10441 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10501 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10561 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10621 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10681 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10741 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10801 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10861 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    10921 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    10981 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11041 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11101 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11161 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11221 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11281 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11341 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11401 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11461 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11521 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11581 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11641 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    11701 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11761 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11821 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    11881 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    11941 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    12001 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    12061 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    12121 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    12181 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    12241 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    12301 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    12361 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    12421 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    12481 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    12541 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    12601 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    12661 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    12721 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    12781 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    12841 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    12901 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    12961 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    13021 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    13081 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    13141 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    13201 atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag
    13261 atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt
    13321 ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac
    13381 accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg
    13441 ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt
    13501 gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt
    13561 gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg
    13621 acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt
    13681 gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg
    13741 accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac
    13801 gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt
    13861 tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata
    13921 attagccacg gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct
    13981 gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt
    14041 tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa
    14101 cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat
    14161 ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg
    14221 cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat
    14281 cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat
    14341 tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga
    14401 ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga
    14461 cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg
    14521 acacattgtc catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt
    14581 cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga
    14641 tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat
    14701 cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga
    14761 ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa
    14821 tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg
    14881 taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta
    14941 tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata
    15001 caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc
    15061 gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga
    15121 cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt
    15181 tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc
    15241 caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct
    15301 gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta
    15361 ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac
    15421 aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct
    15481 tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat
    15541 ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa
    15601 acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag
    15661 ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt
    15721 cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga
    15781 caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat
    15841 cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa
    15901 tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca
    15961 gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa
    16021 tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt
    16081 ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca
    16141 cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata
    16201 atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa
    16261 acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga
    16321 cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat
    16381 cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa
    16441 gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca
    16501 aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt
    16561 tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata
    16621 atatttccag cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata
    16681 gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc
    16741 tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata
    16801 tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc
    16861 gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt
    16921 cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac
    16981 aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa
    17041 ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa
    17101 aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa
    17161 agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca
    17221 tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg
    17281 agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg
    17341 accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt
    17401 taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata
    17461 tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg
    17521 ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc
    17581 aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac
    17641 gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt
    17701 tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact
    17761 taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga
    17821 aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt
    17881 aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt
    17941 ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg
    18001 aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca
    18061 actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg
    18121 acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac
    18181 agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca
    18241 agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat
    18301 gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat
    18361 acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca
    18421 acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat
    18481 atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc
    18541 aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt
    18601 ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc
    18661 agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag
    18721 ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag
    18781 attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa
    18841 caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt
    18901 actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg
    18961 acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg
    19021 gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc
    19081 gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta
    19141 gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa
    19201 acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa
    19261 cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa
    19321 taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt
    19381 aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg
    19441 gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg
    19501 gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga
    19561 atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc
    19621 atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa
    19681 aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa
    19741 ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg
    19801 agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg
    19861 atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt
    19921 gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag
    19981 ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt
    20041 tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag
    20101 cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa
    20161 agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg
    20221 gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta
    20281 aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg
    20341 atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg
    20401 ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt
    20461 ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc
    20521 cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag
    20581 ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag
    20641 cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt
    20701 taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga
    20761 atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt
    20821 ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata
    20881 tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg
    20941 ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga
    21001 ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt
    21061 tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct
    21121 atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt
    21181 ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag
    21241 tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg
    21301 aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta
    21361 ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat
    21421 aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat
    21481 ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa
    21541 gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc
    21601 gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa
    21661 ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa
    21721 ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg
    21781 ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa
    21841 ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac
    21901 tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg
    21961 aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa
    22021 agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc
    22081 gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac
    22141 tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag
    22201 aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa
    22261 atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg
    22321 atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg
    22381 aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca
    22441 acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa
    22501 ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg
    22561 aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag
    22621 gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa
    22681 cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa
    22741 tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg
    22801 atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata
    22861 atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc
    22921 agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat
    22981 caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct
    23041 tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa
    23101 cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa
    23161 gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat
    23221 ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc
    23281 cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg
    23341 ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca
    23401 agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa
    23461 gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca
    23521 gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa
    23581 tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag
    23641 aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca
    23701 aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt
    23761 cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga
    23821 atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt tttttattat
    23881 aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc
    23941 actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa
    24001 taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac
    24061 agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga
    24121 aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc
    24181 tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt
    24241 aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc
    24301 tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg
    24361 aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga
    24421 actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac
    24481 gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg
    24541 agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc
    24601 tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc
    24661 tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa
    24721 atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac
    24781 tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat
    24841 tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga
    24901 agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt
    24961 agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg
    25021 cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat
    25081 tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc
    25141 gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat
    25201 aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa cgtttgaccc
    25261 tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt tggagctgat
    25321 tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta tcttgacaaa
    25381 taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat ttgtcgatat
    25441 cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt
    25501 ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg tagaaccagg
    25561 ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg gatcaccagt
    25621 caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga caacagaagt
    25681 tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat tgatcgtagc
    25741 aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga
    25801 agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag cagcaggact
    25861 tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata aaacattgcg
    25921 tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag
    25981 tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg gtaaagcagt
    26041 agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa tcatcgccaa
    26101 tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca tttcaggggc
    26161 gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc
    26221 agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc cttttgaaac
    26281 actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca ctactcgcgg
    26341 gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa
    26401 agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt
    26461 tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt tcaatgttgg
    26521 gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga tgctctctag
    26581 tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt catgactgat
    26641 cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt
    26701 atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca agaatcacct
    26761 gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga atggggaaaa
    26821 ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt tattttttat
    26881 attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt gatcagagaa
    26941 cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc accagaagca
    27001 aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa taaattagcc
    27061 aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt aggtggtaac
    27121 atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa
    27181 ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca tgaattcgga
    27241 gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata tacgacgaat
    27301 gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa tgaactaaat
    27361 gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga tgaaggtgag
    27421 gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg cttccaagga
    27481 atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac gcgtgcagca
    27541 atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa
    27601 acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag tcaatcactt
    27661 gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa aaatgggcaa
    27721 atttcaagtg atcgatcatc cattgattca acacaaacta actattattc gggacaaaaa
    27781 ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga tggcatatga
    27841 agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca ctgaaacaac
    27901 acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg caggtattgg
    27961 aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata tcggtcttta
    28021 tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag aagacattga
    28081 tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag caatcatggc
    28141 aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc
    28201 acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct atacagcttc
    28261 tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg atgcgggtga
    28321 ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca
    28381 tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc tttcgtttgc
    28441 atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt tttagttctt
    28501 tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt tctgcagttt
    28561 catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca gtttgcgact
    28621 tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca atttccgatg
    28681 gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct gcctttcaca
    28741 tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc catcggactc
    28801 tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct tagctcagtt
    28861 ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag ctggcattaa
    28921 aagagaagac agatatatca aggattttta atccattgat acatctgtct ttttgtttaa
    28981 aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa aaatttagga
    29041 gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg cccaacttgt
    29101 aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca ccccttgtat
    29161 caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac tgtaatggat
    29221 aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact tactagaaaa
    29281 aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag gcaaaataat
    29341 aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc atttcaaaat
    29401 ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa aatatttggc
    29461 tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt ttttaccaaa
    29521 aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa taatggatgg
    29581 cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata tccctttttt
    29641 aagttctatt ttttttccta aaatgttatt catgataata actcctacta ttcagttttt
    29701 gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta tggatatttg
    29761 tattgctaaa aatttatctt atatattcat tttgattata ctacttttat ttgtaaaaat
    29821 tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag atgttccgag
    29881 cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa atcagtatca
    29941 aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag ggaacagtct
    30001 acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat gtttgaacca
    30061 gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat ataactttcc
    30121 atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa catgcttttt
    30181 gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat ctccattctt
    30241 tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat aattggatgg
    30301 cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag ttcaaaatct
    30361 tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt aactttagga
    30421 aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc taaaccaact
    30481 tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt gtcgctatct
    30541 cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc taataaaaat
    30601 cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa aatcgttgcc
    30661 ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat ctagatgtag
    30721 cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg aaatcgtgat
    30781 gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg ttttaaatcc
    30841 agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct tagaaaaagg
    30901 tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt tagcaatcag
    30961 gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa taggaaaatt
    31021 tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc atttataaaa
    31081 acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc aggctaggtt
    31141 catttatttg aacagagtct gtattttttt attctaaata aagaaacaga tgtttcttta
    31201 tattgtattt tatcctttaa ttaacacaaa aacagctagt ataaactaat aatgttaatt
    31261 aaaagtgtat tttatgaaac tatcgttatt tattaatgtt gcatttttgt aagttaaaaa
    31321 ttggaaatat agattgaaaa aatgaaagca ataagtaaaa taaaatgaat atatgcgaaa
    31381 aa
//
LOCUS       DASOUD010000022        31334 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.022, whole genome shotgun sequence.
ACCESSION   DASOUD010000022 DASOUD010000000
VERSION     DASOUD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.022"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..369
                     /locus_tag="U4A29_000950"
     CDS             1..369
                     /locus_tag="U4A29_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO0680087.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="U4A29_000951"
     CDS             549..1490
                     /locus_tag="U4A29_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO0680088.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="U4A29_000952"
     CDS             1559..2290
                     /locus_tag="U4A29_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEO0680089.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="U4A29_000953"
     CDS             2434..4614
                     /locus_tag="U4A29_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO0680090.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="U4A29_000954"
     CDS             4634..5116
                     /locus_tag="U4A29_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO0680091.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="U4A29_000955"
     CDS             5132..5893
                     /locus_tag="U4A29_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEO0680092.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="U4A29_000956"
     CDS             6024..7358
                     /locus_tag="U4A29_000956"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO0680093.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="U4A29_000957"
     CDS             7665..8150
                     /locus_tag="U4A29_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEO0680094.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="U4A29_000958"
     CDS             8143..8826
                     /locus_tag="U4A29_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO0680095.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="U4A29_000959"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="U4A29_000959"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO0680096.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="U4A29_000960"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="U4A29_000960"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO0680097.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="U4A29_000961"
     CDS             12442..13071
                     /locus_tag="U4A29_000961"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO0680098.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="U4A29_000962"
     CDS             13123..13455
                     /locus_tag="U4A29_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO0680099.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="U4A29_000963"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="U4A29_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO0680100.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="U4A29_000964"
     CDS             14281..15654
                     /locus_tag="U4A29_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO0680101.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="U4A29_000965"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="U4A29_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO0680102.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="U4A29_000966"
     CDS             16709..17149
                     /locus_tag="U4A29_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO0680103.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="U4A29_000967"
     CDS             17293..17994
                     /locus_tag="U4A29_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO0680104.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="U4A29_000968"
     CDS             17991..19112
                     /locus_tag="U4A29_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO0680105.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="U4A29_000969"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="U4A29_000969"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO0680106.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="U4A29_000970"
     CDS             20491..21399
                     /locus_tag="U4A29_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEO0680107.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="U4A29_000971"
     CDS             21434..21706
                     /locus_tag="U4A29_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO0680108.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="U4A29_000972"
     CDS             21717..22763
                     /locus_tag="U4A29_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEO0680109.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23014..25623
                     /gene="clpB"
                     /locus_tag="U4A29_000973"
     CDS             23014..25623
                     /gene="clpB"
                     /locus_tag="U4A29_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEO0680110.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25774..26460)
                     /locus_tag="U4A29_000974"
     CDS             complement(25774..26460)
                     /locus_tag="U4A29_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680111.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26438..26932)
                     /locus_tag="U4A29_000975"
     CDS             complement(26438..26932)
                     /locus_tag="U4A29_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEO0680112.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26952..28172)
                     /locus_tag="U4A29_000976"
     CDS             complement(26952..28172)
                     /locus_tag="U4A29_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0680113.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28319..30154
                     /gene="lepA"
                     /locus_tag="U4A29_000977"
     CDS             28319..30154
                     /gene="lepA"
                     /locus_tag="U4A29_000977"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO0680114.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30248..>31334)
                     /locus_tag="U4A29_000978"
     CDS             complement(30248..>31334)
                     /locus_tag="U4A29_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO0680115.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10431 a         5601 c         6715 g         8587 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggtagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa
    22861 tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta
    22921 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
    22981 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
    23041 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
    23101 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
    23161 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
    23221 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
    23281 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
    23341 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
    23401 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
    23461 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
    23521 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
    23581 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
    23641 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
    23701 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
    23761 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
    23821 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
    23881 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
    23941 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
    24001 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
    24061 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
    24121 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
    24181 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
    24241 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
    24301 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
    24361 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
    24421 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
    24481 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
    24541 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
    24601 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
    24661 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
    24721 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
    24781 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
    24841 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
    24901 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
    24961 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
    25021 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
    25081 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
    25141 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
    25201 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
    25261 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
    25321 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
    25381 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
    25441 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
    25501 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
    25561 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
    25621 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
    25681 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
    25741 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
    25801 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
    25861 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
    25921 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
    25981 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
    26041 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
    26101 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
    26161 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
    26221 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
    26281 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
    26341 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
    26401 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
    26461 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
    26521 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
    26581 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
    26641 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
    26701 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
    26761 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
    26821 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
    26881 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
    26941 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
    27001 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
    27061 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
    27121 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
    27181 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
    27241 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
    27301 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
    27361 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
    27421 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
    27481 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
    27541 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
    27601 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
    27661 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
    27721 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
    27781 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
    27841 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
    27901 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
    27961 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
    28021 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
    28081 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
    28141 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
    28201 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
    28261 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
    28321 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
    28381 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
    28441 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
    28501 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
    28561 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
    28621 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
    28681 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
    28741 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
    28801 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
    28861 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
    28921 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
    28981 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
    29041 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
    29101 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
    29161 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
    29221 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
    29281 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
    29341 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
    29401 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
    29461 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
    29521 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
    29581 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
    29641 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
    29701 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
    29761 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
    29821 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
    29881 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
    29941 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
    30001 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
    30061 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
    30121 tctgaaaatg gacgaggacg aaccgaagaa atagcaaggg tttgtttgaa aacagtgtaa
    30181 aaaataaacc atgtttacat ggatttgcca ccggtttgcc accggtggtt tttttcggtg
    30241 gcaaaaatca agaattcttt ctaagatttt ccatgatatc gaccgtttca tttctcatct
    30301 tatctgttac atgcgagtaa gtatccatcg taattgaaat tctgctgtgg cccaatcgtt
    30361 cagagatttc tttcattttt gcgccatttt cgagaagaag tgtagcatga gtatgtctga
    30421 gagaatggaa gttaaaagag agggagagtg cattcgatat cctcctagta ttccatttca
    30481 ctacacttgg cgtaactagc tcaccgtctt ccttcgtaca tactgcattt gaatcaatgt
    30541 agagctttcc atacttcatt cgattttcta attgttgttt cttatgtttt ttcagaattg
    30601 ctagcaaggt ttgtccaata aaaatcgttc gattggaact gcttgtcttt ggcgtaccat
    30661 atacccatgc gccgtcattc tttaccattt gtttctctac agtaattgtt ccatttgaaa
    30721 aatcgacatc atcccacgtc agaccacaaa cttcgccaac gcgcattccc gtataaaatc
    30781 caatattcaa aggaatatag aaaggatgtc cttcaggagt aatttctagc atatgatcaa
    30841 agtcctcaag agaaatgatt tttagatctt ttttagtcgt tggtcgtgct tcatattttg
    30901 gtatttttac atacagcata ggattttgct tgattaatcc ccaaggataa accgccatat
    30961 tcagcgcatt cttaaggaca gagtgagtaa tagtcattgt tttcttcgag taaccctttt
    31021 taaattcagc attgatgaaa ttttgtaaaa gagcagggga gagatccgta agttttttct
    31081 ttcctaaata accgtttata tgatttttga tggtaaatcg gtagttttca taggtattgt
    31141 attttagatt tagtttaacg tattcctcca tccaaaaatc aaggtattgt tttactcgag
    31201 tatccgtacc taaaaagtat tgtcctgttt cgtcaatatc tgataaaact tttcgtaaag
    31261 cagcttcggc ctctggtcgg gtgtctccgc caactttctc cacttttttt cttgagccat
    31321 catcattgat atct
//
LOCUS       DASOUD010000023        31135 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.023, whole genome shotgun sequence.
ACCESSION   DASOUD010000023 DASOUD010000000
VERSION     DASOUD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.023"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(281..1225)
                     /locus_tag="U4A29_000979"
     CDS             complement(281..1225)
                     /locus_tag="U4A29_000979"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO0680116.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1521..2465)
                     /locus_tag="U4A29_000980"
     CDS             complement(1521..2465)
                     /locus_tag="U4A29_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0680117.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2686..3900
                     /locus_tag="U4A29_000981"
     CDS             2686..3900
                     /locus_tag="U4A29_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO0680118.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3922..5937
                     /locus_tag="U4A29_000982"
     CDS             3922..5937
                     /locus_tag="U4A29_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HEO0680119.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6020..7393)
                     /locus_tag="U4A29_000983"
     CDS             complement(6020..7393)
                     /locus_tag="U4A29_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO0680120.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7419..7703)
                     /locus_tag="U4A29_000984"
     CDS             complement(7419..7703)
                     /locus_tag="U4A29_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0680121.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7705..9720)
                     /locus_tag="U4A29_000985"
     CDS             complement(7705..9720)
                     /locus_tag="U4A29_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO0680122.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9899..10204)
                     /locus_tag="U4A29_000986"
     CDS             complement(9899..10204)
                     /locus_tag="U4A29_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEO0680123.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10293..11324)
                     /locus_tag="U4A29_000987"
     CDS             complement(10293..11324)
                     /locus_tag="U4A29_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO0680124.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11531..12406
                     /locus_tag="U4A29_000988"
     CDS             11531..12406
                     /locus_tag="U4A29_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO0680125.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12558..13400)
                     /locus_tag="U4A29_000989"
     CDS             complement(12558..13400)
                     /locus_tag="U4A29_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO0680126.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13492..15369)
                     /locus_tag="U4A29_000990"
     CDS             complement(13492..15369)
                     /locus_tag="U4A29_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO0680127.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15366..16787)
                     /locus_tag="U4A29_000991"
     CDS             complement(15366..16787)
                     /locus_tag="U4A29_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO0680128.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16790..20179)
                     /locus_tag="U4A29_000992"
     CDS             complement(16790..20179)
                     /locus_tag="U4A29_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO0680129.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20436..21827
                     /locus_tag="U4A29_000993"
     CDS             20436..21827
                     /locus_tag="U4A29_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0680130.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21900..22547)
                     /locus_tag="U4A29_000994"
     CDS             complement(21900..22547)
                     /locus_tag="U4A29_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEO0680131.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22555..23241)
                     /locus_tag="U4A29_000995"
     CDS             complement(22555..23241)
                     /locus_tag="U4A29_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680132.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23247..24320)
                     /locus_tag="U4A29_000996"
     CDS             complement(23247..24320)
                     /locus_tag="U4A29_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680133.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24542..25216
                     /locus_tag="U4A29_000997"
     CDS             24542..25216
                     /locus_tag="U4A29_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0680134.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25221..26561
                     /locus_tag="U4A29_000998"
     CDS             25221..26561
                     /locus_tag="U4A29_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO0680135.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26710..27696)
                     /locus_tag="U4A29_000999"
     CDS             complement(26710..27696)
                     /locus_tag="U4A29_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEO0680136.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28420..29451
                     /locus_tag="U4A29_001000"
     CDS             28420..29451
                     /locus_tag="U4A29_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO0680137.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29560..31092
                     /locus_tag="U4A29_001001"
     CDS             29560..31092
                     /locus_tag="U4A29_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEO0680138.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
BASE COUNT         9243 a         5829 c         5625 g        10438 t
ORIGIN      
        1 ttattcagtt aatcaaatat gaacgaatta gatatgaatc attgtatttg ccgttgcgga
       61 aggatttttt aattaaccac agataaacat tgataacgat tcgcttattg taatcaacag
      121 attaaatttc atatgttcat atgtacggat acattttcgg accgattaat tttaaccatt
      181 ctgtttattt gcttacctat tagatatcaa taaaaaactg cttaacagct atttcagctg
      241 ttaagcagtt ttaggatttt tagttcatat tcattgtttc ttataataca gtgttcaata
      301 cttctcttaa agcacttgtt gatttgctga acattgcttt ttcttcttcg ttgattgata
      361 attcaacaac ttcacgaaca ccattttcgt caacgattga tggcacacct gtaaagatat
      421 ctttttcgtc gtattcacca gttaaataag cagatacagg caatacagct tgttcgttat
      481 tcaagatagc ttttacgata cgtgcagtac tcataccaat accatagtaa gttgcttttt
      541 tacgatcaat gatctcataa gcagcattac ggactttatc agcaatcaca tctaattcat
      601 cttgggcaat acgatggtct ttttctacaa tttcaaacac tggtttacca ccgacagttg
      661 tatgtgacca tgcagcaact tctgaatcac catgttcacc taagatatag ccgtggacgc
      721 tgcgtgggtc aacttttaat ttcaatgcga tttctttacg gaaacgagtt gtatccaatg
      781 ttgttccagt accgataaca cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg
      841 tcaaaatatc tactgggttt gaagcaacaa cgataatacc gtcgaatcct gatcccatga
      901 tttcttttac gatttggcgc atgattgacg catttgtttt aactaaatct aaacgagatt
      961 gaccaggttt ttggttgata ccagctgtga taacaacgat atcagcatct ttacattctt
     1021 catagccacc tgaccataca gccacatttt catcgcccca agccatacca tctaaaaggt
     1081 ccaatgcttc cccttctgct ttttcttgat tcacatcgat caatactaat tcattagaga
     1141 ttccttggtt gatcattgca taagcgatac ttgttccaac aaaacctgta ccgacgatta
     1201 ctactttacg acttgttttt ttcatgaata aacagctcct tttcataact atttgttact
     1261 ttcatgaaaa gtatagtctt gtttattcat tttatcaaca tgttaattat aatagttgtt
     1321 attataattt ttgatggaaa cgaattcctg aatacgcata acagataaat gacaaatgtt
     1381 tacacctata tcccctgata atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa
     1441 attcgctgca acgatataat aagacagcaa tcgcacctaa aacaattttc cttcatatgc
     1501 tttagtgaaa gattttcttc ttaatgttta tttctccatt cattgggagt acatgttgaa
     1561 tacttcttga ataatttgaa taaagaagtc gtttgtttat atccgatacg attagcgatt
     1621 tcttccaacg aaaaactcgt ttcttgaatc agttccaacg caaagtcgaa ttttttcttt
     1681 agaaccagtt cttgaaagga agcatcaaaa tacattttca atttattact aagatagacc
     1741 ggattgtagc caaaatgagc tgctgtttct tttaacgtac aagaagtaaa atgcttatcg
     1801 atataatcta atactctaag catctcattt tctgcgcatc catctgctga tgaaaagctt
     1861 tgttgttcta ataaatgagt caattcgatc agtaatagtg atgtatagag atttaacgaa
     1921 cggatcttat gactgtatgt atcaaaataa atcaacagca tattttcaat caaatgcgtc
     1981 aaacgacttg tttttccgcc gcgaaaatga atgaattgtg tatgttcccc tttttctttt
     2041 gatgcattta tcaaaaaaga agaaagtaga ttatttgtgc ttgccaaatg attcaatatc
     2101 tctgtagata aggtttcttc ttttaataag atgttgatta aaatatcctc ttgccctgtg
     2161 tattcgattc tttgagtagc ttctttatca atgatcacca cgtcactttc ttccatctct
     2221 atcctttctc cattaataat ttgtaaacat gtaccgctat acatataatt gatttcaaca
     2281 aaatcatgtg tatgctcggg aacttccgca tatcgacggt gtttactgat atgaatattt
     2341 ccctgttgaa atagatcccc tcggtggatt tttggcggtg tctgaagatg gtaagctaag
     2401 tttaaatcat tgatattcat tccttgttca cgctgcagag tttcgattga ttcttcgtta
     2461 cgcaaaaaaa atgtctaatt cagctagttc caattgactc cctccttttt gtaagcggtt
     2521 tctttcgctt attagttcac gctgtactgc tattatatgc aaagctattt ttggtttcat
     2581 aaagactttc tatggttggg acatgaactt cctgtgattc cgctattgag agaataacca
     2641 cttcatacta tatttttgat aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa
     2701 aagatgatga aattttctct gctgtcagtc tctcttattt tgacttctgc tggtgcagtc
     2761 acgtctgcaa ttcccagtat gtcagctact tatgccacgc gttctttatc tgctgttgaa
     2821 ttattaacga ctgctccctc ttttatggtc actatttttg ttttgattag tagttttatt
     2881 gctaggaaaa tcggacaaaa acaaactgtg atcatcggat tagtgattgc atcgatttat
     2941 ggtatcctcc ccctcttctc acaaagttac agtattgtct tagcttcaag agctggacta
     3001 ggtattggct ttggattgat caattctttg gcagtcagca tgattgctgc cttttatcac
     3061 ggagatgaac gagcctctat gattggtttt caaagtgcat ttcaaggatt tggtgcagct
     3121 ttaatgacat ttgttgcagg acagctagta cagttcggtt ggcaatatac ctttttagtc
     3181 tacgccatca gcttacctat tcttgtcttg tttgttttgt ttgttcctac acctgaaaca
     3241 actgaacttc aatcacatgg cacagcccca acaagacaag taatcaaaac gaatcctaaa
     3301 atgattagtt atggtatttt tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt
     3361 aaattagcca cagtcctgac gacttattct ctaggaaacg aaactaatgc agcgaatatc
     3421 ttaacaatca tgcaattggc aagtatggct gccggagttt gttttggctg gcttcagaca
     3481 aaagcacatc gctatatgct tacactttct ttactactga tgggaatcgg ctttgtattg
     3541 attgccactg tgataaacct gtatgtgatt gggtttggtg cgatcctaac aggaatttct
     3601 ttttcgttat tcattcctta cttattcaac caagtaagta tccaagcgcc caagtacgcg
     3661 gagagtttca atacttcaat cttattggtt ggcgctaatt taggtacttt cattgcacca
     3721 tatggtttgc tctttctctc ttttgcttct cttgggagta aaacacttgc tgttttcgtc
     3781 aatggcagta tcttgctgtt gattagcgcg cttattagcc ttctagtagt acacaggacg
     3841 aatagaaaga cagtgataag agtagatgaa gaaataaaag aacaattgac aacaaaataa
     3901 atttgatgga ggtcgtgtaa aatgacaaat gaaaaacata aagatagatg gctttggtat
     3961 cctggagact ttgagatccg ccatggactg ctacaaaatt tccaacggga agaacgaggt
     4021 tttgactggc cagcgtactg gtacatggat gattgccatc ggaacgtcaa gtttaagaga
     4081 tattactttc ttgatcaacc ttccatgttt aaagtgacga tccaaggagt tgggtatgta
     4141 gaaattaatg gacaaaaaca tccatgtgga aaatggctga cttgtccagc tggtaaagca
     4201 aaaattcgta tctttgtagg acatacatca gggctaccag cgatgtttat cgagggagac
     4261 gaagtaaaat cagatattgg ctggacagca agtaatttta ttgaagaata tccagctggc
     4321 tggagtccgc tttatgtcga tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa
     4381 tgcgtccatc cagtcactga aacggagatt ggcggaggaa tcttattcga ttttgggcga
     4441 gcagtgaatg gctgtgtaga actaatatct ctggatgacc atatcgaatc tatcactcta
     4501 tgttatggag agtccgatgt agaagctatg gatatcgatt attgctacta taagcaaata
     4561 ggaatgacaa gaaaaatgaa agctcgtaaa cgcgcttttc gctatttgtt tataccagag
     4621 gtttctactg gaatggttca acttcaagtt tttcatgaat ttttatctct ttcgaaacaa
     4681 gctgcttttt caagcgattc ttctttactg aatcagatct ggtcagtatc agaagaaacc
     4741 ttcaagttat gtagtgattt gttttttatc gacggtatca aacgagatcg ctggatttgg
     4801 tctggagatg cgtatcaatc attcatgatc aatcaatatc ttttttttga cgaggaaatc
     4861 aataaacgaa cgatattagc attaagagga caaaatgaaa tcaagcagca tatgaatact
     4921 atcgttgact attcgatctt gtggttgatt ggtatcgaaa accactatat gatgacgaaa
     4981 gactatgaat ttttacgcat cgtctaccct aagatgcaat cattaatgag atacttgatg
     5041 gagcaaacga atgaattagg gtttatttat gggagagcaa aagattggat atttattgat
     5101 tggtctgaaa tggataaaga aggaacgctt gctgcagaac aagtcttatt actgaaagcc
     5161 tatcattcca tgatcacttg cggagatatc ttagctgaaa catatggaga gaacgaaaat
     5221 tatcatactc atctctatca agaaaaatat caactattga aacaaaacct atttgactac
     5281 ttttgggatg aagaacttgg cgcttttatc gattgctatg aatctgggag aagaaatgtg
     5341 acgcgtcatg cgaatatttt cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa
     5401 tcgatcttaa agaatgtctt attgaatgac actatcacac agattactac tccatatttc
     5461 aaattctatg aacaagatgc tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg
     5521 attcttgatt attggggagg gatgttatct catgatgcgg taactttctg ggaagaatac
     5581 gaccctactc aaacaggaaa tgaaaagtac agtatgtatg gagatcctta tggaaaaagc
     5641 ttatgccatg catggggagc aagcccaatc tatcttttag gacgctattt tatcggtttg
     5701 tatccaacaa agccaggtta tgaagaattt atgatacaac ctaatctaga ttcatttgaa
     5761 acactgaatt gtacgctacc tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat
     5821 aagatcgtga ttacttctga tagaacaggc ggaacagtga aaatgaatgg taaaagttat
     5881 cctcttctag caaaacagcc acttgtctta acaagctatt caaaaataaa tgcataaaac
     5941 aaaaaactgc ttgcgacata tcagaagact ttaaatcttc ttctgatatt gtccaagcaa
     6001 tttttatttt gaagtttgct taattctgtt ctttagaaac aatgtttgtt ctttttccgg
     6061 caaaaagaaa gctagataat acgacaagta aaaagaaagc gatgattcca actgtcaagg
     6121 ctatttgacc gtattctcct agtttcccaa gtacaagtcc tggtactaga taatcagtat
     6181 cggcaaatgt agaagaagcg attttaagat ctcctaatac tggaaggata aataatggca
     6241 gccaactaat caataagcta ttgaaagcag aacctaatac cgcccctctt ctaccgccag
     6301 tcgagttgcc gaagacacca gaagcagctc cgcagaagaa atgtcctact accccaggaa
     6361 tgataactgt agtttgcaag cctatcatag caaacatgga tagtgttcct acaaagaagc
     6421 taacaaaaaa gccaattaat acagcattag gtgcatatgg aaaaacgata ggaacatcta
     6481 atgctggttt agaatcgggt acgagttttt tagcaatccc ttggaatgct ggaacgattt
     6541 ctgccaacac cattcgaacc ccttgaagga cgataacaaa cccagctgtg aatgttccag
     6601 cttggatcag tgcaaagatt acagcgttgt ctccattgct tagttctctt tcaacaaatc
     6661 caggaccagc taacaaagca agtgcgacat atacgatcga catcaagagc atgatcgata
     6721 cagtagaatc tcgcaaaaag ctgaaacttt taggaatctt tatgtcctct gtagaacggt
     6781 ctttgtttcc aaaaaacttt ccgatccatc cactagaagc ataaccgata tttccggtat
     6841 gccctaacgc aacttgattg tttccagtga tgttcttcat aaacggttga caaagagcag
     6901 gcgtaatagt taatagtgtt ccttcgaaga ccccgcctaa taagatcatc cacaaatttg
     6961 cagtaaaccc cgcgcttact aaaatgactg ctgttagaca agaaacataa agcattgctt
     7021 gacctgtaag aaaaatgaat ttatatcttg tgaatctagc aagtaaaata ttcacgatca
     7081 tccctactag catgatcaaa gcagtaggtg taccgtattg ttgcaaagca atcgccacta
     7141 ctgcttcatt gctaggaact actccttgag tatgcagagc tgtttggaac atcgaagcaa
     7201 aaggggttag tgaattactg attaaaccag caccgccgat cagtactagg aatccgacaa
     7261 atgttttgat agttccttgg atcacttctg aaaatttctt cttctgaaga acgagaccaa
     7321 gcagtgcaat caaagaaacg aggatggagg gctgactgac gattgacaca aacaaattca
     7381 acaatttatc cattgatagt tcctcccttg aatcattttt attcttttga caagatcttc
     7441 gttacttttt cttttacttc ttcgctgtca atgatcgatt gcaaaggtac gagcttttca
     7501 tcaggtatag aagttagcgt atctttcaaa gaatgctcaa tgaagaaata atcagccata
     7561 tcgctagata cacttccaac atccgtatga cttacttcaa actcgtccac atctgctcca
     7621 agatctgcaa gtgcttgttt gatgttcatc tctaccataa aacttgttcc taatccactt
     7681 tgacatactg caaaaatttt catatcattc gtctcctttt cttatgatcg ctaatatttc
     7741 ttcttttgtt gatgcgtgta ataaatcttg aagattttgt ttattagaaa gtagctttgt
     7801 taaatttgcc aaagcccgaa gatgtgcctc attatcgata gcagctaaac agatgaacaa
     7861 actaatggga tgggctggat tatcaagcag atccacaggt tgttgaagtt tcagcaaaga
     7921 catccctact tcgtttacgc catcttctgg acgagcatga ggtaatgcga tgtgatcgcc
     7981 aatatggata aacgcaccat actgctccac gcgctcgatc atagctttga tataactttc
     8041 ttctatttta ttttctgata atagcggctg tgcggcaaat gtgattgctt cttgccagtt
     8101 ttttggttga tccgataatt ggatcatttc ttctgttaat aattcagata gcataggacg
     8161 ttgatcctcc ttcttttcta aaagtttgtt cattttacga tttaatactt gatagagctt
     8221 ttcttcgtct acgccttctt ttaattcgat ataaggcagt agcgccgaca acaattcttg
     8281 ggcagaaggc attgaaaatc ctggtatcag ccaatctctt tgtacttgat tgatcagctg
     8341 atttttttcc aagtcagtca tgattggatg cagaacatag agagactttt tcttatcagt
     8401 aagtgtgaga ggtacagtag aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa
     8461 gcggtcagct gaattagtct ctgtaaaaaa gatcatcggg aataattgct gaagttcact
     8521 ttttaagatc aatgaagagc taatgccact tggacagacg atcacggctc ttatttttct
     8581 atttctttct tctgcgtccg cttttctgat ctctccgcca aatagtatag tgaaaaaacc
     8641 aatctcttct tcagggatgc tcttccctat tcgtctttct aacggaagta atgcttttct
     8701 tgtcatttcg tagatggaag catattgatg cttaatttga tcgatcatca cattaggtaa
     8761 ataaaaagaa taggaaatac gaaaataggc agggaccaag tgataaaaga cattcatcaa
     8821 taattcacga aacgaagcaa attctacggc catcaggaat tccatgcgat ggatgacttc
     8881 tgaagaacat tccagcaaaa aatcaagtgc aggctcctgg acatctcctt caacgatcga
     8941 tgctaagcaa agagagaaaa atataatctc cgaattttga tgttcggaat agccaaggcc
     9001 agtcaatact tgtcgactaa tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt
     9061 taccagttcc atggatgttt ttccaattcg aatggttgtc aaactcaaat aataagctaa
     9121 ttcatccatt cgatttggtg cgaatcgaag atctgatagt gaaatcgttt tatctagcaa
     9181 atctctaact ttggcgtaat aatcaatgga aagggttgcg gttaaatacg tgagcccaaa
     9241 tcttccagca agtgactgca tcaaacgagc agtgcgattt tttgcttctc ttcttaatat
     9301 tgcttctgac cctagcagcc taaaaccagt tttcctagta tacgacagct gacaatcttc
     9361 cacttctaat tcatttctta tctttttgat atctgacagg actgtccctt tagaaacctg
     9421 aagaaaatct tgaaaatgaa aaacagaaac agacgttgca gtcgaataaa ccatcaaata
     9481 aagaagatct cttcgctctt gttcggtata cacagcttca aatcgagtaa tatccaaaaa
     9541 tttttctgcc cagtcgattg ctcggaattt ctttatacgg atacgggtat catccacata
     9601 ttcggcaaaa ggagttaatt gtttgatcgc ctcgcttgta tttacttcga tgatctctgc
     9661 tgctttttct agactgattt ctcttggtgt tttcaggaga taactaattt tgtagttcac
     9721 agcgctgttc acctcctact tatattctag tatggtagcg cttttaaatc gaggggtgtt
     9781 tggtacaact tacagttgga ttttttttac gactgtaccg aacgatttct atcgaatgga
     9841 tacagaaaaa aagcaggtga ccaaatgtct cctgctttta tgaattgatt tcgtccgatc
     9901 aatgatgatc atgtttattt tttgaggtca gttcttcaaa tttttctttg acatcttcaa
     9961 ccgctttttc gatatctttt tcaaagtgat tgggctcatt aggctttgtt tcctcttcta
    10021 cagaatttga accagataaa gctgtttctt tattttctgt ttcaccagag tttgtgccag
    10081 ctaatgattc ttctttgtct tctgtttcac cagaattagt tcctgctaaa gccacttctt
    10141 cttctgtttt aggggccatt gaacctctta atcgtttgtg tttttcaccg tcataatttg
    10201 tcatgttggt tcctcctagt agttctatac tttttcatcg tacaataaga cagtttatgc
    10261 gtcaaaaaac aaacccgatt cttacaaata tgtcatattt ttccttctga ttcttgaatg
    10321 cttttgattc cttcttccaa aattttcatt tgtaagattg tttcttcatc tttgatgact
    10381 ttttctgcgc cattaactaa tgtctcgtaa atcccttcgt agacacggct gtaatcacca
    10441 acctcggaaa ttactttttc ttcatgataa ctcccttgtt catctatata ggtcaataca
    10501 ccataatgtt cgggcaaatc gataccaaag tcttcatgat caggcatgta aaacattttc
    10561 agatgttctt cttgtcgatc ttttgtttgt ttcacgaaca tccctttttt tccgtaaacg
    10621 acgaaacttg gacgttcttt gatgcggaaa taactggatt ttatcgaaat tttcatattt
    10681 ccatagaaaa aatccagatc gaagtaatca ttcattcgtc ctttccctaa aagttgacgt
    10741 acatcatagc tgatatcatc tggttcgccg aaataagaaa gaacttgatc tactgtatga
    10801 caagcatgtc catataaata gctgttcgca gtagaaaatt catgcgtatt ttctggtact
    10861 tccggtctga aataatcata atgcatttct acttctaaaa tatcccctaa ttttccgctt
    10921 tcgattactt tttggactgt caagaagtca gaatcgaacc gtctgttttg gtaacattgg
    10981 ataaatagac ctttttcttt tgctaatgca aataactctt ctgcttcctt tgacgtttct
    11041 gcaaaaggtt tctctactag tacattctta ccatgttcga gtgccatttt tccatattca
    11101 taatgaaatt gactaggtgt agtgatgaca actaattgga tctcaggatc atcccaaaga
    11161 tcagacagct gatcagtata ttcgatgtct tcgaatgttt tccacactgg ttcagatggg
    11221 gaaaaaattt ttttgatatt gattttgttc gtaagttttc ttgaaaaagg caaatgatag
    11281 cgatttgtac tctttccatt gccaagataa gcgattgtaa gcatgagtgg tgcctccttt
    11341 tattgatgtt ttcagtatat cctagaaaag cgcagaaagt atttataata gctattctgg
    11401 taaaaaagtt cagaaaaaca cttgttatca ttcggtttgg tgaaattaat gttcaaagta
    11461 aggataaaaa tcaaaaagat agtagcggaa ccaagtgttt atcagtaaga tagaatttgg
    11521 aggtcagatc atgcttattg aagaaaaact aacaaaacaa gagttgttta ccacgacaga
    11581 gaaacggatt gtcgattaca tccgtaggaa tatcgaagct gcagtctaca tgacgattga
    11641 agaattagca aaagctacgt atacttccca ttcagctatc attcgtttat gcaaaaaaat
    11701 gggattcagc ggatttaaag aattccgttt cgagctgggg cgggaagttc atcaattgat
    11761 tgctcagttc aatcaaacga ttgatcctaa ttttcctttt tcaagtgaag atagcactca
    11821 gcaaattgca aagaaaatgg cggatttatc gatccagtca ataaaaaaag cacaattgca
    11881 gattgaacat gaagatttag atcaaatcgg taaagtattg aacaaggcac agcgaatctt
    11941 tctttttgca aaaggtgatt ctcaaatcac ggcaaggaaa ttccagaata aaatggtcaa
    12001 gttgaataag tttttgatca tggctgaaga atacagtgat tcttcttgga atgcagccaa
    12061 tttgactagc gaggattgcg ccatttttat tagctacagc ggtcgcatcc atcattatga
    12121 acgaataatg acctatttga agcatgtaga ggcccctacc ttgctgatta ccggaaatca
    12181 gcattcagaa atggccaaac aggctgatat gtgtcttgtt atatcgcagg aggaatatga
    12241 ttttgctaaa gtagcgactt tctcctctca aattgccttc gattacgtat taaatacgtt
    12301 attctcagtg atatattctc aaaattttga agagaatatc cgcaacttga aaaacaaaca
    12361 agaattgatg caaaaaggcc ttcttaaaga atatcggaaa acttaagaaa agatcctcat
    12421 ttagtaataa gaaagatagt gtttgatagg ctctctttga tatcatcata aaaacaggct
    12481 gaagttttct ttttcaaacc tcagcctgtt tgtttacgga acgattcgaa tagatatatt
    12541 atagaaatga ggaatagcta acttctgttt cttattttga acgattgacg aaaaagatat
    12601 ataagaagga ggaaaatcag tatacatgca gctaacaatc catacaggaa aaatcgtttg
    12661 ttctcggata ctttctgtac ttctttttga acttcttttg catgataagg aacccggtgc
    12721 cctctgacaa gtaagcgatg actgttgacc atataaggtg tacaagttag gagtgtcacg
    12781 aaatcctgcc ctttttcgat tctcagatct tttgtatcag tcggttctac cgtcttgatc
    12841 tgatcgactt gataagcgag tgtccgtcca ttgatttcga tatagaaaga atcacctaaa
    12901 gtcagtttcg gtaagtctgt gaacaacttt gcttgaggca gtcctcgatg gctggaaata
    12961 acggcatgtg tattttctcc ccccgtagga taagacgttc cttccaataa tgaggcacct
    13021 ttttctagca gcacagtatt cgtttcatca aaaattggca aatggacatt gatctcaggg
    13081 atcgtcaaaa taccgatcgt atgtttctca aaataggttc tatccttttt cgcaggttct
    13141 tttttcttag taaagggatc tgcacctgga tttccacctt cttttgctaa ttgctggttt
    13201 ttctttgtca tgttttcttg gattttagcc atgacttctt cattttcttt gaccgcttgt
    13261 tgctgataat gagagatgat ctgctgatcc agataattat tcagcgcgtc gctaacaaat
    13321 ggataagcta gtatccctat tcctgagaga agcaagaaaa gcatcagcag atctagaagt
    13381 ttccttttct ttggtttcat ttgtgttctc cttgtgatga ctgtttgata ataaaagaaa
    13441 aaggagcatt tgcgtaatcc gctgctcctt tttccaattc ttgcaaaaag actaaatctg
    13501 actgtgcttg cgtctagcaa agtacagtcc agcaatcagt agaaggacta ctcctgcacc
    13561 gatatacaca tagattccct taccgcctgt tgaaggaagt gtacctttgt gtttatttgg
    13621 tattttttca ggagaaatca actgtcctgc tgttacataa gattgttcat cgatagtaaa
    13681 tgctacacgg tttgttaatg gaacatattt ttctggcgct ttcgtttctt ccagatagta
    13741 cgtgccatat tttagacctg tcacatcgat taaaccgtta cttgtggttg taaaaaccgt
    13801 tgctgattct ttcgccgata cccagctgac ggcttttgtg gatgggtcga tcgataaata
    13861 tttcgctgtg tcactatctt gatcacgaac gacgaattct gctccagcaa gtgtttggtc
    13921 tgatgtaacg tcaccatcta ctttaacaaa tcgtttgccc ccagtaacga catcgactga
    13981 cggtggtgtt tgatcatttg tatgcccgtt atcgacattt gcttggttag aaaatccttt
    14041 ggttggatct gctttttcgt tcaaatgcat atagtaaacg aatttcaatg taccgccagg
    14101 agttaatgaa ggaatataat tcggatcaac tgaaaccgtg aatccgtctg tttgctcagt
    14161 gacagaataa tttactgggt cgatttcttt atttccatca tataaggcat aagcatacgt
    14221 tcctgaagaa tcattatcaa atgttaaagc agcgtcatga gtatcgataa gtttgaatgt
    14281 tgtgtacttg ttttgcgttc cttctttatc agcgatacct aatgggatat tgacagaaat
    14341 ctcatattga attttttcac cgattgcgac atcttttcca ttcaattgag gtgttgtttt
    14401 atcaacttta gacgtatcat ttttgatggt cttttctact ggtgtttggc tatttgccag
    14461 gatctcaaaa ggcgtgattg tttgcttttc aatcatttcc gcattatcag gagcttttac
    14521 ttcttctaaa ttatattttc caacttctaa atgattaacg atcaactggc ctttttcaat
    14581 agatgcctcg gcaaagtcat tgttgccgat gtcataagaa tgaccagtaa tgaaatgttt
    14641 ggctttggtt tgatcagttg tccaagtgta caatccatct gtgacacttt ggatgtattt
    14701 tttgacgctt ggtgttcctt cttctttaga aataataaat tctgctccat ttagtgcttc
    14761 gttttcggca gtaccgattt ttgtaacttt caacgttcca tcattacaga ctgtattttt
    14821 agggtagaga tggatagtat ctagttcttc tgtcccgtat ttataagagc catctgcttg
    14881 tttgatcatc tcatatacag ggaaagctaa aaccatgttt gcggcagctg acactccgtc
    14941 ttttggttct tctttgatcg tgtagactgc atctttccca ttttgttttt taggtaaaga
    15001 taaagtgaca tttccatcag catctgtcgt tcctgaagca actggtgtac caggagtcaa
    15061 agactggact gcttgttttg ctgcatccac ggacgctcct ttatctcgtt gcgcataaaa
    15121 ttcttgagtg acgttataaa ctgaaaatga aatatcggct aatccttggt attgatcgaa
    15181 ttcgctcatt tctttcccgc tgttttggat taaaggatcg ggtaaatcag tcattttctt
    15241 tttatgaagg atgacttgag ctgctgtctc ctctgcaaac actttttttg cgccgaagct
    15301 gtttgtgagt aatggtaaaa taaggaaaag gactcctaac ataacgttta tttttttatg
    15361 atttttcatt ttttcactcc tgttcttttt ttgataaaca accagattgc aaagcttgcg
    15421 aataccatac caacaacgcc taaaagtgta tttttcgttt cgtttgtttt tggaagaaat
    15481 ggacggctat tttctttttt attgactact ttcaatatac tgtccgcctc tttatcctca
    15541 gaaagaaaag ttgaaatggt aaacggaatt tctgtttcac ttaaaatata accagaaggt
    15601 gcttcgatct cttttaatcg ataacttcct gttttcaacc cggaaattga aaaagcgcca
    15661 tttttatcgg aaatcaggtg aagcgcatct gattcgttct ttgtccactt gtatccgttg
    15721 gctgtttgct ggaggtaatt tcctgctttg tttttaataa ggaatactgc gccagctaat
    15781 cgtttatctg gtttatttgt ctcgattttg acaaattgct tactccctgt atatacagag
    15841 actttctttg tactgacacc aaaaccaaaa tccagtgttc cgttgttgtt gatttccttg
    15901 ttcgcctgtg ctgtactgct tagctgcatc tgataagaaa tggtcagttt ttttccagca
    15961 tatttttcca acgaagggat cgaaaataaa acatcaaatc cgtgaggttt cttgtgtatg
    16021 acaaagcctt ctgtcagctt ttctccattc gatgaaatcg ttaaactatt aggtaaaaac
    16081 gttaatgctt catccgcact atctgacaat tcgaactttt gatagtcaag gatatttaca
    16141 ggaattgtcg tatgcaagga ataagtgatt ttttcaccaa tcgtaaaatc atttctaggc
    16201 tcttctaata ccttctcaaa aggtggatca gggtagtcgt tttcttcatt tttaggatac
    16261 aaatggatag ttgaaagttt ttgattgttt tgtccatgta caggaagaac aactaccata
    16321 ttctctgcct tttctttgac gacttctggt actttggatt caatgaaaag atacgcttta
    16381 tctctttttt cttgatcttt agcggccaga gaaaaagaag cgataccatc ttcgttgtta
    16441 agagttgtag ttgttgcttc tgcggtaaac ataccggaag acgcaccgtt ttttgcgatc
    16501 tctgcttgtg cttcttctac cgttttgccc ttttcccgta gatggtaaaa agaatctgtg
    16561 acatcataaa cttggaatgt gacaccattt aatcctcgat acgtttgtaa taaagctttt
    16621 tcttgtccgt catttggatg atttttcggc agttgcccgt ttgggaaaag caatttgtgt
    16681 aaagtaatct gaaccatttc tgtttctgtt tctgttgcta cctgagtaaa agctggttta
    16741 aatagtaaca agaagagaca catactaagc caaccaagtt ttttcatatt tagctgcccc
    16801 ctctttcttt tttcaaaaaa agataccaaa tagaaaacgc acaaccaatc atccctacta
    16861 gatagattcc taaacggcca attcctcccg tttcaggtaa tggtactttt gcctgattcg
    16921 tgatgtctaa agaaatctgg ttgtttttgg ctcctggtga cagaacagat tcatgatctt
    16981 gtccatccac ttgaattgac ccatcttcgt gtataactat agtaactggc tcttttagac
    17041 cttggtatcc ttctggtgta aaagtttcgg tcagcgtata agttccaggt tttaaatttt
    17101 cgaatagaaa ggtatctgtt tcttgtccat cttttggcga ttctaagtcc atttctggtc
    17161 cctgcagtct gaattttgct ccttttaatg tctgtccgtt gtcttctttt ttgtgtaccg
    17221 ttagatcaaa cgcttttagt cgattttgat gagtgagcgt ccatacatta tttgtcatct
    17281 cggtattttt tcccgtgtaa atgatttttc catatttatc gatttggaat tcataatttc
    17341 ccggcgaagt atcatatccg gcaggaccag ctgtttcaac gactcgatat ttaccaggtg
    17401 tgagtgaatc aaaactggca agaccttttt cattcgttgt ttggctgtca attggctgat
    17461 aagtaccatt tgcttctttt ttctgtaggg aaaatgctgc tcctttaaga tttatctctt
    17521 tgtccgtccc ttgcatcgca tacttacgta ctgctacagg aacttcaaca aaaggatttt
    17581 caatagtcaa ctggatcgta tcatcggaag tagtgactgt ttttccatca atggttaccg
    17641 taccatcaga agcgatcttg atttcccaag tagtcttttt gcttaatcca tgcccttctg
    17701 gagcttttgt ttctgtcagc gtataggtca tttctttttg caatttgaca ttttctggaa
    17761 gagaataggt tccgtcgcca tgatctgtta gtaaagtatc aatagaatct cctgttaatt
    17821 tgaaaacagc gccaataaga tccttttcac ctgtagagga atttttcgtt attttcaagt
    17881 ttaacggtgt gaattgttta gtatttttcc atgtcattgc atctatttgt tgagaatcgt
    17941 atcctggtac aggtaattct ttgattgttt ggtaactgaa tgcttgacct tgattattgt
    18001 attgaggtag tgataatatc tcttgataac tttctccgct atttgcagat aatttgtcaa
    18061 tgtctgcacg ttcccacgta tttgttgtat ctttagctgg tttaatgatt cgaatatatc
    18121 cgtttttcca agctgcagca tttgtcgtat gttcccgttg aatctcaaaa gtaacttgat
    18181 ctggacgatc agctagattg ttgtcaaatt cttcccataa ctttttgatg tgaagactga
    18241 ctccgggagc tttagcagat ggtataccga attcagctaa ttcattggta tcaatgtttg
    18301 gctggaaagt tgtcctgccg ttcatttgat accagaaatt tggatggaag tcctcatttt
    18361 ctgtttggat tctcacttgg taatggattt ggatttcttg gtcttttcct aaatagatct
    18421 gattgctctt gatggtattt ccttctatgg aaatagatgg agtgaccgtt gtaggacttg
    18481 tccccacact cttgactgaa agtgtaccag gctgataaat gaatggttct gcgattggat
    18541 catttatttg tccattgctt acagttgctg agatctgtac agcttttttg gctaggtatt
    18601 cagagatatc tgttgcatga tcagctgatt catagtaaag atcgcctttt tcatctgatg
    18661 aaaccatttg tcgcatacga gactctactt ctgcttttga gagaccagct gccggatcgc
    18721 tttgaagttg gatgccaaga ccatgtattt cgattcctcg ttctttgagt gccatcgctt
    18781 ctccgatggt tgcgataaac gtactgtcga ttcttctgga tagattgttt tggtcaggtg
    18841 catcatagat tcttgagatt agagaagtat ttcccggccg atcttgcgta ttagaaaact
    18901 gagttccgta ataattgctg cttgattgtg cgtgtacccg ctgtacttta taggaaaatg
    18961 ttggtacacc atccgtcagc aaaacgatca cttttttatg tccatttgga acggatagca
    19021 tgcttcctgc atctcttagt gctttttgtg taaaagtacc accatttgtc cgtgaaggtg
    19081 taatggattt tacttgattt ttcactgaat caaatgaacc catctgtact gcaccgttac
    19141 tgtagctata tccttcgctt gagtagccga catatcccat attgattttg tctgtgatac
    19201 cgctatctgc taaagtatcg acaaaacgat cgacaccaat ctttacttca ccgatccgat
    19261 tattgtcgtt catacttcct gaccagtcta cgaccaatac gagatcaaga ggcgtgatat
    19321 ctttttgtac atttccacga gcattcaaat aaacatcaaa caatccgggt gtacttgttt
    19381 cctttgcata ctttcgtatc gcatagtctg cttgattacc ggttcctcca tattcgatat
    19441 acgaatgggt ccgatcatta ggatctccat tccaagaatt tataccatcc cattgatttg
    19501 ttccgttttt attgccttga tgatcaagaa catttgtatt tcctgtaggt tgccaactgg
    19561 ctttaggata agtgccctga tcatctgttg tatattctgt aggaatcgtt tgattagatg
    19621 gcacactcac agctggcaag gataatgtag gcaatgtaaa tgggctgaca gaattttcgt
    19681 tttcttttga tgattcttct tctatttcgt ctggctttgg tactgacaca tctgtatctt
    19741 tcttattttc tgtctcatca ataatgtcat attctttatt atcgacgatc agcttgtttg
    19801 cctggctgtg gtaaagtgct ggatcgattg gaaaactcag atcgatagtt tgtttagtgt
    19861 ttgatgagat ttccaacgta attttcccgc tatttacctg ataagcatag ccatctcctt
    19921 cttttctttc cacatcactt aagtaatttt cgactagacc aatttctttt tctatctttt
    19981 cagcactggt gttattcgca tttagcttga tgttcagtgc tttttttcca gactgatccg
    20041 aaatgacttc agctgaaatt gtttctaaaa ctgttggatc atctgtagtg tctgctattg
    20101 cagataatgg cacaaagttt gataaactca atattatcag catgaaaata actttcagtt
    20161 tcttccctgt ggtcgtcatt tttcccctcc tttttctttt tttctgtttt gacgtgaata
    20221 gtgaaaatta ttaaaataaa caggcgttac ataattttat ttattttaat atgataatta
    20281 ttatacgaag ggcgctaaag ggaatcacac caattttcat accaattggt ttcatttatg
    20341 aagttgattc cttcgattaa tgagcgtttc attgaaagaa aagggttgag ggagaaaaac
    20401 atatgatcag gttgttggaa tgaaactata gaaagatgga aaaaatcatt cgaattctgt
    20461 ccatttctga caagtggatg actaaaaaag aacttgccaa acgaattggt agttcagaat
    20521 ctacatttgt aaggtatatc gaagagatca aaatccgctg gggaacggat gtaacgattc
    20581 aatcttcaca aaaattgggg tatcgtcttg aacatttgaa tgtaccagct tatttaaatg
    20641 ttctgatgga aatgtcgcaa agttctacaa acagtcgttt attaaatgag attattgtca
    20701 atccgggtaa aacgattgag tattattgtg aaaaaatttc catcagccgg tcttctttta
    20761 cacgtaagtt gaaacaatgc aatcaaattt tagaaagcta tttattaaaa gtgatcgttg
    20821 accaaggttt tcagttaact agccttaaaa gtgagctcca gttgcgtatc tttgttacct
    20881 ttttcttttt aagttactac ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga
    20941 tcaaagaaat tatggtcaag aataattgtc agatgaattt gtttcaggaa gaaagcccat
    21001 atgagcaatc tttctttatt atgtattttg ttgtgcatct catgagagaa tcccaaggaa
    21061 ctccatttga atggaaagct ggagactctt atgtacagtt gataaatatc gataaaaaag
    21121 acttcttaat cttgaaaaat ctcttaggaa aattgaagta cagcagctat ataaaagtag
    21181 tgaactattt tgtcaatacg attttttcag aattttcgga gaaagaaaaa aatgaaatca
    21241 gtgagcagat acgaaaaaat atgtcctact gttcgttttc atccgctgat tgcaaagaaa
    21301 aaaacgaaga atatgttgtt caattattaa cgcaaattta ttttttctcc cgtattttcc
    21361 catacgatac gactcatttg acgatccgtg caacttactt tgcacagcag atgcaacacc
    21421 gccagatacg tctatatgct ggatttcgtg cagaacttca attttattcc actctactga
    21481 atcagaatct gatttattat ttatctagct atatcttttg gatgcttatc ggtaatcctg
    21541 aaattcacca ttatacttct gataaaaaaa tactgattat cagcgactta agtcttcgac
    21601 atagtcaata tattgaagaa tatatcagtg atattcttgc tgtgcataaa attcactctg
    21661 agacaactgc tattactgaa gatcagctgt cgaaatacaa tcttctcgag tatgatctga
    21721 tcgttacgaa tcaacccatt ttgaatgccc atgttcctca tatattgatt gatgatagtg
    21781 tgtcattcgc aaatgaagaa gaactcatcc gcttattcga gttatgaata atcaaaaaac
    21841 agctatgtat aaaccaccct gataaaaatt tcaggacaaa tacacatagc tgtttctttt
    21901 tattgtattc cttcagcaat ccaacgagat ttatagcgaa agtcttttaa caactcttct
    21961 tttgtccaat tatttgcaat gagatccgtc aaagtgttca ttacgagggt catggaaaag
    22021 tataggaaac taactgcttc ttccgacgat tgaatggaga aatgattgat tcttaataat
    22081 tccagccatt tttcgttcat tggagaatca gccgatagtt ctctacgttt ggttgtctgc
    22141 tgatcatttc gctgggttaa caagtataac ctttgccaat agatactttc gtgatctaag
    22201 tcgcagcacc actctaaata tttttcgatc cccaaaaaga aatgttgttt gttttcgcta
    22261 atgaatttta agatatcttg atggactatg tgagaactgc ttttgatcgt atacaaataa
    22321 gcatcttcca aatcttgaaa atatttataa aaagcaccgc gcgacatccc catcttgtca
    22381 acgatttctg agactttgac ttggctgatg tgctgtgagc aaaatatctc cagtaatagg
    22441 tcatcgattc tctgtttctt tgcgttcgat aaattgaaaa aagtttcttt tggcatccac
    22501 atctctcctt tatctctgat tatacgtgga tagcccttca ttgtcatgaa ggattcattc
    22561 cttctttttc tcttcttctg tcaagtaccc atcttccatt cgataaacgc gatcactcca
    22621 ttgaatcata cggacatcat gagtaaccat aatagtcgct ttttgtttct catgagcttc
    22681 tttaactagt aatttgacga cttcatatgc atgatctgta tccaaactag ctgttggttc
    22741 gtctgctaag atcaagcttg gctcattata taaagcgcga gcaattgcca ctctctgtct
    22801 ttctccacca gaaaggtctt taggataact atttttaagt gactggatat ctaaagattg
    22861 cagcaaggaa tcgatttttg ttgaatcttc tttttttgaa gtaacttggt caactagttc
    22921 aaattgttcc tttaccgtta aaaaaggaat caaattagag ctttggagaa taaatcctat
    22981 ttctttaaag cgtaaatctg ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt
    23041 gatcactatt tctccggaag tcggtgtttg caatcctcct gaaatggtaa ggaaagtact
    23101 tttacctgaa ccagaaggac caatgatgct gacgaattct ccttccttta cataaaaatc
    23161 aattgctttc aaagctgaga cctttgtatg accagaacca aatgttttgg taactttttt
    23221 catcgttaat atttttttca tttttattaa cctcctattg ctgtaattgg gtctactttg
    23281 gttatcgtac gtatcgaaaa caatccgcct agcattgcga caatgatcaa aatcccacta
    23341 tatagcaacc attgtgacca aaaaacagca aaaggcatcg cgcttggtaa aatcagacta
    23401 gtcaaataag caaacaaaag agcaagcaga acgcctacca ccccaacaat aaaggactgt
    23461 gctactaaag atttagcgat gaagaaattt ttgatccctt gtgctttcat tactccaaaa
    23521 atagcagtct tctgaagtgt catcacgtac atgaaaatac cgaccacggc agcaaccact
    23581 aagaacaaaa agtatatcat cgcatttaag gtcatatttt gggcagaata gccaggaata
    23641 ctttcaatga atgtcccaat agttaataac tgtagaccct tcgatgcttt ttctttcgaa
    23701 attttagctg aatcctttat tacgatccca ttgatcggct gttcttcttc agaagcaaac
    23761 ggctgttgtc catacttcaa agcagtccac gtatctaggg acgcatagat gacgggagaa
    23821 actgtgtaat aagtttcagg aaagataccg acaatcgtca aaggatcgtc gtaactgcca
    23881 attttgatcg tatctccaat tttatagcct gaatcagcca gattttgaga gatgatgatt
    23941 tcattctttg ttttaaattc atgccctttc gtgagttttg gtaataaaaa tgcttctttc
    24001 gttgtgccga aaactgtaac attttctttg ttttttcctt tgattgctcc gctgtacaat
    24061 ccgattggtg ctttctctcc tccttcaatg tatttcagat ctcctcttgt taattgtgaa
    24121 gctgcaaata cttgatttgc atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg
    24181 attgcttttt taaactcttc tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca
    24241 tacgcaatca agaacataat accgacgatc aagccatatc gtaatttaga atagcgaatt
    24301 tctttgatag ctagaaacat ttgtttttcc tccctatgat agattgatag tgacaaaatg
    24361 tcacttgttg aatagtgtaa ctctaaagtg acaatttgtc acttgtgttg tttttttctt
    24421 atcaataaat tgtctaagac atctttcgat aaaaccttaa catgagtcat aaaaaattta
    24481 tttaagcctt atttatgctt tccatgataa actaaagtga ccaaatcaat gaggtgggaa
    24541 aatgattaaa ctattagtag tagaagacga taaagtatta tccaataata taaaagaaat
    24601 actcgctgca atcggcgaga taacacaagt ttatgatggt agtgaaggac tgttcgagtt
    24661 ggaaagtgga gtttatgatc ttgcaatctt ggatttgatg atgccggaaa tgaatggata
    24721 tgaagtgtta gcaaaaatac ggaaagaaaa aatacagact ccagtattga ttttgactgc
    24781 aaaagatagt ttggaagata agattgaagg atttgaacga ggtgcagatg attatctaac
    24841 aaaacctttc tatcgagaag aactgatcat gcgcgtgaaa gcattattga aacgctcgct
    24901 aggtctcttt gatgaaaatg ttctgaagta taaggatatc agttgtgatt taaaaagcaa
    24961 tgaagtcttg tataaagaac aagttttgcc catacaagga aaagagtttg aattgctcgt
    25021 ttatttttta caaaataagg gaatcatttt gacaaaagag cagatatttg accggatctg
    25081 gggatttgat tctgatacga cgattacagt tattgaagtt tatatgagca acttacgaaa
    25141 acatctgcga agtactggat tggataaaga aatcaaaacc ttacgtaatg tcggttatat
    25201 actgcaaagg gtgtgatgca atgaatggag atattacacg aaagcaacgt agtcgctttt
    25261 ttgcgacaaa tgtagcagca tttgctttta tcttttttct tttgggattc attactttac
    25321 agatattaaa ccaatctgca tacagagaaa cagatctgtc attagagaaa ataaattttg
    25381 attctcgaac agtacaatta gagatacaaa gatatcagca aggcgatccc tttttaaacc
    25441 cgactcaaac ggaacaaaac agaacaaaca tggaacagcc attaatggaa ccatcaaatg
    25501 accgttttaa cactcaagtg atcttgtggt ctaaaaatgg tgagatattg aataaagcag
    25561 ctattggtgg gcgtttcaac caaatcaaag aattaaaaat ggatacgaaa aatctgaata
    25621 ccatccaaaa tattgaatta aatgaagaca gtggctcatc tgccctctct ttccgttcga
    25681 ttacgaaaaa agcggagacg aatcaagatg gcgttgccta catccaaatt ttagaaaata
    25741 caaatcaaat caagaattca ttagcgactt ttcgcacgat tatgattttg tgtatgattg
    25801 ttttttggat tctctctatt ggtatcagtt attacttgtc tagtctaagc atgcgcccta
    25861 ttattctttc ttggagaaga caaaaagaat ttgttgagaa tgcttctcat gaattgcgca
    25921 caccgttaac gattattcaa aatagtttgg aacatctttt taccaagcct aatcatacta
    25981 ttattgaaga atccgaaagc atcgctcaag cattgtcaga gacgaggcgc ctcactggat
    26041 tgacttcgga tttgctgaca atcgctcgga atgattctga tcagcagctg ctttcaaagc
    26101 aaatgatcaa tacacaagaa tatatcgaac aacttgttaa accatttcaa gagatggcca
    26161 tcatagatgg aaaagagttt attctccata acgatgcaac attagaagta ccaattgatg
    26221 aaaaaaaaat ccatcaagtt ttggttattt tgctggataa tgcattaaaa tatacgaatc
    26281 cagaagataa gatcattttg gattcatctg ttaaccataa agaatggctg attcacgtaa
    26341 agaatactgg cccttctatc agtgaagaag atcaagcaag gatttttgat cgattttatc
    26401 gggaagatca atctcgggcg aaagagactg gcggatacgg gcttggcttg gcaattgcaa
    26461 aacaggtaat cgaacagcat catggacaga tttctgtatc caattatctt ccacaaggag
    26521 tcgattttaa agtccaattg ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg
    26581 gctgtgacaa aaatcttgtc acagcccttt tttgttgcga ataaacggtg ttccagaagt
    26641 agcttcttcg gaaataagcc gaaatttctt taagctgacc acttctgtca caacctcttt
    26701 agcttacgct tacgattttt tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat
    26761 atccaattct tctcggctaa catcttctgt cactttttca acatcttcat gttcttcttt
    26821 atgaatccca acttcttcac gaataactgt atgtttgctt acatctactt gctcttcttt
    26881 gattgggata gtaatcgtgt catcttccaa tttttcgtct gttccctgtg aagattgatc
    26941 tgttactggt ttgcgttcga tgacgatttc ttcacgtttg accggtactt ctaccgtttc
    27001 agtatcgttg accacatgtt tgtggatgtc gacttctcca gttgtcacat catgtgtatt
    27061 tacatctaat ttttcttctt ttagttgaat tgtatcatca tctagatttg gtttgcgatc
    27121 gatgtcttca gtcttgcttg tacccattgt atcagtctcg ccaggtacgt ccatcggatc
    27181 agttcctggt gtcatattca atggatccgt tgtattttga aagcctgtcg tgccacctac
    27241 gtccattgga tctgttccag tgccagtttc taatccagtc cccattgtgc ttgctgggct
    27301 aacagtatct tgtgtatcca ttgtatcttc tgttgtttct ggctgacgat agccttttac
    27361 tgtgatgaca aattttccat cagaaatatc tcctctatat tgatataaag gatcattttc
    27421 tggtgtagcc gtctcagaat cataggtatc aaaagaaaaa gtatctttta ttttttccca
    27481 taaagaacgg tcttcttcac catccatccg attttcatct gtttcaacat ctataccaga
    27541 caatccgtca aatcctagtc tagatgctgc ttcatcagta gtataaataa cgatatcatc
    27601 tcgttcatac ccttcgttac gcaaccgttc aattactgat aatgcttctt ccctcgtact
    27661 atagcttccg atgaccgtta aatttttctc ttccatgtaa attcctccta gcaaatagat
    27721 ttatgattgg tatattgtta cttataaatt tattctatag gagacgattg ttttctacaa
    27781 atatttagat tggaatttga ttaaaacaat ttaattgaaa tttgaaaaca tttatgttaa
    27841 tgaaagtcat attctaataa aatgtcattt gtttctatat gaatttttat gaaaaatttc
    27901 atataatttc cactcgttgt gatggtttaa tttatccaat ccatcaataa gcaaacaact
    27961 tatataaaaa aggattaatt ctcaatataa ttgctcctac catagaataa atcttcatgt
    28021 aaagtaatga agtaccaact atttctaata cttctttaag aattatccca acaatatcgt
    28081 tagacaaacc attattgaat aaatggagag ccaatcgatt ctgaaaaaat gttttctaat
    28141 ttttcggaaa taaaagaaag agcctcctgc cattagaacg aaccaaatcc atttcattgc
    28201 tcatgaagta accacatagc taaaaagagg ataaatcaat taaaagatca aaagaatgac
    28261 tgcactaata aaataactaa aaatgtatct tcttacttca tatcttttag ctagtactta
    28321 gttaaaaatt tgcttcctaa gatcaagaca aactgtctat cttcatttga tgaaatgcgg
    28381 taaaatatag aaaatgaaca ctattggagg gaatttgtaa tggacagcaa aactagaaaa
    28441 caactgcaag aaaagttaaa gcaacgtaca cacttgctcc cttccgtctt tcaacagttt
    28501 ttagaagaaa atcgttcttc cctttcatta ggcagtcgct atgaatacgc aaaagatttt
    28561 catctgttta ctgattattt gagaaaagta ttttctgaag aagtggatga tcagctgatt
    28621 atccatttag aaaaacaaga ttatcagaat ttcttagcta aaatctatcg acatactcgt
    28681 tcgttccaaa ctacagcgaa ccaagaaacg gagcaattgt ttgaaaatag ctattacggg
    28741 atttcaagga agcaatattc attaaatcat tttttacgat ttttgtatca aaaaggactg
    28801 attgaagaag agatttctct tttgggtaca agtttaccgg aaacaaccaa agcagctccg
    28861 cgatatatcg atgcagaact tttcaaagat tttgagcatt tgtttatccc gattgaaggc
    28921 tttcaaacaa gcagagaagc tagctttgaa gaaaagaata gaccgaggaa ccttgcaatt
    28981 actgccattc ttctgtattg cggattaaag atccatgaag tagtagaact taaacgtaaa
    29041 gatgtatctt tgaccaagca gattcttaat ctgaatagaa aagaaaacag actgaataaa
    29101 gtaccaatac caaaagaagc gatgccattt ctggagaact attttaagtt aacagcacaa
    29161 aatacggacg aaaattcttt tgtttttcgt tcctcacatg atcagcaaat ttctcctcgg
    29221 actattcgcc aaattttgag caaaattaca gtatataaaa acgtatcgcc agaactatgc
    29281 agaaaaacat atcgttatta tgcagaacta tatttagatg atgctgacgc ggtaaattat
    29341 ttatgtggta atcgatacct ctcatcccat tcttttcaag atatctggga aaagatggtt
    29401 gatttttctt atcacgaatt agcggcattc gttcgggttt cttccatata aaacaaatgt
    29461 atgatctttt tttcacaata aactatgtta ttatataaag gattatgtta cgattatctt
    29521 gaatttgtat attacttaac aaaagataat ggagtgtgaa tgatggagac cataacgtat
    29581 tcccctttga acttatttac gaattttcaa gaagctgcag ctaacttttc cgagaccccg
    29641 attatctttg atgagccact ttcagctttc ccagaattag gacttgaaac aacttacaaa
    29701 aaatgctgcg aaagtgtgtt gaaacgggct tatcaattgg ctcatcttgg agtgaaatcc
    29761 ggagacaaag ttattattta taagagttcc gcttttgaca cataccttct agctgtttct
    29821 gtgtcttacc ttggtgcagt gcctgtcatg acttcttatc atctaccaac aacaacaatg
    29881 gaagtattta tcgatcgttt agaagaccct ttcattttat ttgatgatga gacaaaaaag
    29941 cgagtgagag aaatttcaaa tggaacaaaa tcaaaacaga ttcctattct ttatttaatg
    30001 gaacaacctg ctttgtctgt ttctcaatca tttttagaca aaaatgaaat ttcttatatg
    30061 acccatactt ctggtacaac tggtatccct aaacttattt gtcattctgc tcattctatg
    30121 ggatggcgga cgaaatggca aaaaacagtt ttcacaaaaa tttcggaaaa gaaattaatc
    30181 gcttttcaca tctctccagt gcattccaga ttcaatattg gtatctcttc tttgatgtcc
    30241 atgggatttc ctatgatgcc tttagcaaac gcacaaagtt caaaagttgt tcatatgttg
    30301 gaagctcacc ggccaattgc actagagacc catccgaata actttgttca gtggcgtttt
    30361 actgctaagg agcatccaga agctttttcg ggtatccgat actatcactc cacttttgat
    30421 gctataaaca accagacaat gaagacattt ttgcgaacgt cgatggcaca ggatgcggta
    30481 tttcttcaag tatatggcca aagtgaatgc ggaccaatga ttcttaaggc tcatacacta
    30541 gagagtttga aaacaagtga tgcaagagat atgggcgttg gactggaaga catgacaagc
    30601 gctcggatta cagattcagt tgggaatgtc ctacctgaaa acacagatgg tcatatacaa
    30661 ttactgtcta aaggacgggc tttgacttat tacaaggaag atgcacggtt ccaggaaaat
    30721 gtttatggcg attggtggga tagcggagat tacggtttta tggataagca tggccaccta
    30781 tttttgaaag atcggcaagt ggatttgatc gaaacgatca atagtacatt agctattgaa
    30841 gactttttat tagattcgtt agattttttg gcagaagtag tgattgtaag ggataaaaat
    30901 cattctccac aaccgattat tgctttagct cctgataaag aaatggattg gaatcgatgg
    30961 tgggagcaag tccatgaact tcctcgcttg aacgtcccta tcatccgtga ttttgatgca
    31021 atcccgcgaa cagctacaat gaaagtgcaa cggcttcaaa tcgagaaaga attaaaatca
    31081 caaaagtcct aactatttaa ggaaagaaag atccattttg gtctttcttt tcttt
//
LOCUS       DASOUD010000024        31065 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.024, whole genome shotgun sequence.
ACCESSION   DASOUD010000024 DASOUD010000000
VERSION     DASOUD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.024"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(61..2424)
                     /locus_tag="U4A29_001002"
     CDS             complement(61..2424)
                     /locus_tag="U4A29_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO0680139.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="U4A29_001003"
     CDS             2872..3093
                     /locus_tag="U4A29_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0680140.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="U4A29_001004"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="U4A29_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO0680141.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="U4A29_001005"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="U4A29_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO0680142.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="U4A29_001006"
     CDS             complement(5894..6205)
                     /locus_tag="U4A29_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO0680143.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="U4A29_001007"
     CDS             complement(6202..6495)
                     /locus_tag="U4A29_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO0680144.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="U4A29_001008"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="U4A29_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO0680145.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="U4A29_001009"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="U4A29_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO0680146.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="U4A29_001010"
     CDS             complement(8333..12685)
                     /locus_tag="U4A29_001010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO0680147.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="U4A29_001011"
     CDS             complement(12897..14606)
                     /locus_tag="U4A29_001011"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO0680148.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="U4A29_001012"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="U4A29_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO0680149.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="U4A29_001013"
     CDS             complement(16103..16903)
                     /locus_tag="U4A29_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO0680150.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="U4A29_001014"
     CDS             complement(16900..17712)
                     /locus_tag="U4A29_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO0680151.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="U4A29_001015"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="U4A29_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO0680152.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="U4A29_001016"
     CDS             complement(18467..19189)
                     /locus_tag="U4A29_001016"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO0680153.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="U4A29_001017"
     CDS             complement(19325..20206)
                     /locus_tag="U4A29_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO0680154.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="U4A29_001018"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="U4A29_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO0680155.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="U4A29_001019"
     CDS             21446..21925
                     /locus_tag="U4A29_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO0680156.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="U4A29_001020"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="U4A29_001020"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEO0680157.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /locus_tag="U4A29_001021"
     CDS             23226..24011
                     /locus_tag="U4A29_001021"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEO0680158.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="U4A29_001022"
     CDS             complement(24357..26048)
                     /locus_tag="U4A29_001022"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO0680159.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="U4A29_001023"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="U4A29_001023"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO0680160.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="U4A29_001024"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="U4A29_001024"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO0680161.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="U4A29_001025"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="U4A29_001025"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO0680162.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            30332..31033
                     /locus_tag="U4A29_001026"
     CDS             30332..31033
                     /locus_tag="U4A29_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO0680163.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8390 a         6625 c         5503 g        10547 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
    30241 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
    30301 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
    30361 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
    30421 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
    30481 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
    30541 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
    30601 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
    30661 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
    30721 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
    30781 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
    30841 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
    30901 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
    30961 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
    31021 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatt
//
LOCUS       DASOUD010000025        30342 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.025, whole genome shotgun sequence.
ACCESSION   DASOUD010000025 DASOUD010000000
VERSION     DASOUD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.025"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(84..2159)
                     /locus_tag="U4A29_001027"
     CDS             complement(84..2159)
                     /locus_tag="U4A29_001027"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO0680164.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2161..3078)
                     /gene="glyQ"
                     /locus_tag="U4A29_001028"
     CDS             complement(2161..3078)
                     /gene="glyQ"
                     /locus_tag="U4A29_001028"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO0680165.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3464..4276)
                     /gene="recO"
                     /locus_tag="U4A29_001029"
     CDS             complement(3464..4276)
                     /gene="recO"
                     /locus_tag="U4A29_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO0680166.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(4419..5318)
                     /gene="era"
                     /locus_tag="U4A29_001030"
     CDS             complement(4419..5318)
                     /gene="era"
                     /locus_tag="U4A29_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO0680167.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5333..5728)
                     /locus_tag="U4A29_001031"
     CDS             complement(5333..5728)
                     /locus_tag="U4A29_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO0680168.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(5712..6188)
                     /gene="ybeY"
                     /locus_tag="U4A29_001032"
     CDS             complement(5712..6188)
                     /gene="ybeY"
                     /locus_tag="U4A29_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO0680169.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6205..8397)
                     /locus_tag="U4A29_001033"
     CDS             complement(6205..8397)
                     /locus_tag="U4A29_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEO0680170.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8421..9392)
                     /locus_tag="U4A29_001034"
     CDS             complement(8421..9392)
                     /locus_tag="U4A29_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO0680171.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10098..11477
                     /gene="brnQ"
                     /locus_tag="U4A29_001035"
     CDS             10098..11477
                     /gene="brnQ"
                     /locus_tag="U4A29_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO0680172.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11633..12079)
                     /locus_tag="U4A29_001036"
     CDS             complement(11633..12079)
                     /locus_tag="U4A29_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEO0680173.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12106..12282)
                     /locus_tag="U4A29_001037"
     CDS             complement(12106..12282)
                     /locus_tag="U4A29_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO0680174.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12444..12872)
                     /locus_tag="U4A29_001038"
     CDS             complement(12444..12872)
                     /locus_tag="U4A29_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEO0680175.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12964..13824
                     /locus_tag="U4A29_001039"
     CDS             12964..13824
                     /locus_tag="U4A29_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO0680176.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13918..14268)
                     /locus_tag="U4A29_001040"
     CDS             complement(13918..14268)
                     /locus_tag="U4A29_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEO0680177.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14284..16011)
                     /locus_tag="U4A29_001041"
     CDS             complement(14284..16011)
                     /locus_tag="U4A29_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO0680178.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(16225..17151)
                     /gene="trxB"
                     /locus_tag="U4A29_001042"
     CDS             complement(16225..17151)
                     /gene="trxB"
                     /locus_tag="U4A29_001042"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO0680179.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17296..18732)
                     /locus_tag="U4A29_001043"
     CDS             complement(17296..18732)
                     /locus_tag="U4A29_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO0680180.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     ENEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18729..20132)
                     /locus_tag="U4A29_001044"
     CDS             complement(18729..20132)
                     /locus_tag="U4A29_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO0680181.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20362..21264
                     /locus_tag="U4A29_001045"
     CDS             20362..21264
                     /locus_tag="U4A29_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO0680182.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21305..23191)
                     /locus_tag="U4A29_001046"
     CDS             complement(21305..23191)
                     /locus_tag="U4A29_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO0680183.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23288..24139)
                     /locus_tag="U4A29_001047"
     CDS             complement(23288..24139)
                     /locus_tag="U4A29_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO0680184.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(24462..26246)
                     /locus_tag="U4A29_001048"
     CDS             complement(24462..26246)
                     /locus_tag="U4A29_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680185.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26246..27997)
                     /locus_tag="U4A29_001049"
     CDS             complement(26246..27997)
                     /locus_tag="U4A29_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0680186.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(28139..28363)
                     /locus_tag="U4A29_001050"
     CDS             complement(28139..28363)
                     /locus_tag="U4A29_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO0680187.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28614..30200
                     /locus_tag="U4A29_001051"
     CDS             28614..30200
                     /locus_tag="U4A29_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO0680188.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8446 a         6563 c         5436 g         9897 t
ORIGIN      
        1 ttaataacag ctatttatat ttataacaaa aaaacagaga cacagcacgt ttcgtgcatg
       61 ctctctgttt ttcaaacaaa agactatttt gtgatgattt gatccaagct tgctaatgac
      121 aaagccatgg cagctatttg actaagttgt gttagtcggt tcttacgtac tgctggttca
      181 tcagccatga ccatcgtttg atcaaaataa ttttcgatca gtggacgaag agcgacaagc
      241 tggtcgtagt tttcgtggat cgtttggtca gcaaaacttg ctttcattgc ttctactgct
      301 ttgtgtaatt ctttctctgc gtcgttttcg aacagctcag gatctacttc tgattgtttt
      361 tcaagctgcg cttttttcgc taagttcacg acacgtgtca aagcttccat tgaaggtttg
      421 aagtcttcat ctgcaagatg ttgtttgaac aattgtgctg ctgcaaatac ttttgtcaga
      481 tctttttgtt ccgcattcaa aacagcatcg atgacatcat gacgaatatt cttcgttgtc
      541 aataattggc gcagacggcc tttgataaag tcgatcactt gttgttgccc ttctgataaa
      601 ccaatgccga aacgatccgt atctttactg ataacatcgc taatcgtttc ttgcagaaca
      661 gacaaaggaa aggcccattt tttcgattcg atgatccgaa cgacaccgta tgcttggcga
      721 cgtaaggcat aaggatcgtt tgaaccgctt gggatcatcc caacagcaaa gaaagaaaag
      781 atgctatcta atttatctgc aatcgctaaa acagaaccta ctgtagactg aggtagctct
      841 ccttcactgg atataggcat gtaatgctca cgaatcgctg tcgctacagc aggtttctcg
      901 cctttcagca gagcatattt ttcgcccatg atcccttgca gttcaggaaa ttcccctacc
      961 atgttggtta ctagatcaaa tttatagatt tcagcagctc gttgcagatc catcaattct
     1021 tcctcagata gtcctacttg ttcgccaatg atacgggcaa tgacttgtac ccgttgcatt
     1081 ttctcataga tactaccgat tttttcatgg aaagtaacag tttttagttt ttcaacgtat
     1141 tggtcgattg aagctttctt gtcttcatta taaaagaatt ccgcatcttc taaacgagcc
     1201 gtcaatactt tttgattgcc tcgaaccaca ttttccaatt ttacccgatc accattacga
     1261 gcagaaataa aatgaggaag cagcatacct tgatcattcc gtacctcgaa ataacgttga
     1321 tgttctttca tagaagtgac tagtacttct tcaggaacag aaagatattt ttcttcgaag
     1381 tcgccagaaa agacagttgg atattcgact aaattgttga cttcttctag taaatcttcg
     1441 tctagatcaa taacccaatg atttttttcg gctaattcat tggcttgtgt aacgatcatt
     1501 tcttgtcttt tttgcggaga cgcaatgacg aattgttcat tcaatttttc ttcatattct
     1561 tttgcatgcg caaatgtcac atcttcgcct aagaaacgat gacctcgaga ggctctgcct
     1621 gttttgacat ccaagatttc gaatgggatt acttcgtcat ctaacaatgc cacgatccag
     1681 tgaatagggc ggatatattc aaaatcgtat tctgcccaat gcatcgttac agggaaagtc
     1741 aggcttttaa tgacatctgc taaacctgtc aatacttctt tggattcctt accatgagtg
     1801 tatttcgtta catagacata ttccacacct tttaattctc taaaagtgat agcgtctgtc
     1861 gtcagacctt gtccgcggac aaatccttcc gctgctttcg tccagtttcc atctgcgtcc
     1921 aaagcaattt ttttggctgg tcctttgact tcttcttcac tgtccgcttg tcgttctgga
     1981 atgtttgtta ctttgattgc taaacggcgc ggtgtggaaa atgtttcgat cgtatcataa
     2041 gtcaattggt tttcatctaa aaattttgct gttttttctt ctaattgttt catgcttggt
     2101 gtgacaacat gagcaggcat ttcttccagc ccgatttcaa gtaataagtc ttttgccatc
     2161 ttattcagcc tccttcacgt tttctggatt tgctgtgttt ttattcaaca aagggaatcc
     2221 taatttttcg cgttctgcca caaagatttt ggctacagca cgagccatgt tgcggatgcg
     2281 agaaagataa ccggcacgct ctgttacgga tactgcaccg cgggcgtcta ataagttgaa
     2341 tgtatggcta catttcaaga tataatcata cgccggatga acaagatttt catcgataca
     2401 gcgtttagct tctttttcaa aacgatcaaa gttgcttagt agcaagtctt ggtcgcttac
     2461 ttcaaaagaa tatttagaat gttcatattc tggttgtttg aagatctcgc catatttcac
     2521 gccatcagcc cattctaaat catagacact ttcaacttct tggatataag aagcaagacg
     2581 ttctaatcca taagtgatct cagaagttac tggtttacaa ggcagtccac caacttgttg
     2641 gaagtacgtg aattgtgtga tttccattcc gtcaagccat acttcccaac ccacaccggc
     2701 acagcccatg gatgggttct cccagttgtc ttcaacaaaa cggatgtcgt gttctagtgg
     2761 atcgattcct aaaaggcgca agctttctaa ataaagctct tggatattct caggtgaagg
     2821 tttcatcacg acttggaatt gatgatgctg atacaaacgg tttgggtttt cgccataacg
     2881 tccatcagct ggtcggcgag atggctctac gtaagcagca ttccatggtt ctggtccgat
     2941 tgcccgtaaa aaggtatatg ggctcatcgt acctgctcct ttttctgtgt catacgcttg
     3001 catcagcatg cagccgtttg atgaccaaaa tttttgtaat gttaaaatca tatcttggac
     3061 ggtcaatttt ttactcatct gttttgcccc tttcaatttt aaagcgttgc tgtcaaataa
     3121 gaatacagga aagaaaacga caaaaaatcc ctatgtctga ttacagacat agggacgaat
     3181 aattcgcggt tccaccctac ttccgaataa agattcggca gcttcattta tcgtactcgc
     3241 aagtgcctct tcaaaaatcg tttgggtccg gctcccactc tcccgaactc gctaaaccac
     3301 gtgtttttta ctgatcttgg tcaatgtacg tattcatttt gcagttttct tgtttctata
     3361 atagatcctc tcagtgaacg atctatttga tctacggtta ataatagacg gaactccctg
     3421 tgtttgtcaa atggttttga ttttttgttg tatgtttcat ttactattct tcttttttag
     3481 gtagatcttt ttcagacatg tctttctctg acgtctttaa tacgtgtgcc caagacttca
     3541 tttgatcaat gaactttttg cttttcaaat ggataccgac atattcatca tacagctggt
     3601 cgatgagttc acggattgct tgtttggttt cttctttcag atggattcca ctgattttat
     3661 cataagaaat agctgaaaac atccgaacga aataaacagc tcgaggatct gcatgataac
     3721 ggtgatcatc catcgaccag tgtctttcgc atatcacacc accatatttg gaagaatagt
     3781 caaattttcc ttttgtttcc ccacatacga cgcagtgtgt ccagattggt gcaattccga
     3841 agcgttgcag tatttggact tcaaaaatat tcgtgatgat ttctgcatca tctccttggt
     3901 ccatccgttg cagtgcttgt tgcgtaaact ggaacaaatg cgggtcgtac tgatgatctt
     3961 cgattgccgc atctactaga ttcaataaat atgtgccata gccgctcaaa aagatgtctt
     4021 cttggatgtg tcgaaacgcg tggacatctt tgacactgct taaaaatgac aaaccttcac
     4081 ttcgaaaatt cccgatataa actgcttcag taaaagggag caaagccgga gctaagggct
     4141 gattttgccg atggatccct ttgatataaa acatcatttt gccagaagat tccgtaaaga
     4201 ttttgactaa tttatctttt tctttgtaat tacggctaga taaaatcaag ccttttgatt
     4261 ctgcaagctg gctcatattc tccctcctaa aaaataaatc tggaaaagca gattctgccg
     4321 atccagatca tttatgttgc gatcttccgc agcttttctc aacacgcgga tttttatcca
     4381 gtttatttct atacctaact tacaagaagc tcagaatatc aatattcgtc ttttcggtag
     4441 ccgaagtctt gcagatagac ttttttgtct cgccagtctt tttgaacttt gacccaaagt
     4501 tctagaaaga ctttgtctcc taaaagattt tcgatatctt ttctcgcttt cgttcctact
     4561 tgcttcagca tttttccgcc tttgccgata atgatccctt tttggctatc tctttcgaca
     4621 ataatcgtag cctggatatg gattttatca ttctcgtttc gcttcatcga atcaacaacg
     4681 actgcgacag aatgaggcac ttcgtctcgt gttaaaaaca agactttctc acggatcaat
     4741 tcagaaacga tgaagtattc aggatgatca gtcacttgat catctggaaa atattgtggg
     4801 ccctctggca tttcactgac taatacatcc atcaaacgtt cgacgttgtt tccttctgta
     4861 gcagaaattg gaatcacttg tgcaaacggc atctgcgttg aataatcttc gatgattccc
     4921 aacaactcgt ctggatggat tttatcgatt ttattgataa tcagataaac aggagaatgc
     4981 acgtttttta gacgttcaat aataaaatcg tctccccgac cgcgtttttg atcagcgctg
     5041 atcataaata agaccgcgtc cacttctttc aacgcactgt aagctgcttc taccataaaa
     5101 tctcctaaac gatgctttgg tttatggatt cctggtgtgt caataaaaat caactgagct
     5161 tctggtgtag tatatactcc ttggatttta tttctagttg tctgcgcctt atcactcatg
     5221 atggcaattt tttgacccac gattcgattc aataatgtgg attttcctac attcgggcgg
     5281 ccgacgattg cgacaaaccc tgatttatgt tctggcatat acgttctcct ttttaattaa
     5341 acaacaaatg atatatttta gggacaaata aaatcaaccc aacgatgacg gaaaaacaag
     5401 cggtcactaa aactgctcca gctgccatat ctttgatttt tttcccaatc ggatgaaaat
     5461 gaaaatccgt aaacatgtcc acgacatttt caaaaacagt attgatgatc tctactatcc
     5521 atacgagaaa aacagctaaa agcagccata accattctat ccgatcaagc tggaaaatca
     5581 cgcccataaa caaagcgagg attccgaacg caacatgttt cctcatgttg cgttcttcct
     5641 caaaaacggt tttgactcct tggacggcaa attccacgga catcaggaaa tgtttgtttt
     5701 tctctacgtt tttatctttt aagtccatag gcatctaaga tctctttctg taaaccaaac
     5761 atttctttct catcctctgg tgtcatatgg tcatacccgt tgatatgcaa gaatccatga
     5821 accgctaaaa agccaagttc ccgatcatac gaatggccgt aagcttctgc ttgctctgca
     5881 gcacgttcag tagagatcat gatgtctccc agtacacgag gcaatggatt ctctccatct
     5941 tcaaaaatca ctgggatttc gtcttcgccc tcttcttcca aggcaaaact gatgacgtct
     6001 gtcggtgcat cttttccacg gtaatcacga ttgattactt ggatcgctgc attgtccatg
     6061 aaagtaacag acatttctgt ttcttctggc aactctaaat atgatgctgc aaattgcagc
     6121 aagtcgtcga tttctttcat tttctcttct gagacttggt ttgtttcatc catgaaagta
     6181 atatccatta tacatcgctt ctttctattc ttcttgttct tcttttatct tctcaacttc
     6241 cgatttcatt ttaggatatt tgatccgaga atggaaggta ctgcttaaac cgctgatcaa
     6301 tgatttttct actttacgga tttcttttaa cgtcaatcca ctgtcgtcca attgtccatc
     6361 ggaaatacgc tcttcaataa gattatggac aaaactttcg attttttcgc tcgttggatg
     6421 gtccattgct cgtactgccg cttcacaact gtcagctata ttgacgattc ctgcttcacg
     6481 gctttgaggt tttggtccag gataacggaa ttgctcttcc gtgacttccg ggttgcgttc
     6541 ctttgcctta acgtagaaat aacgcatcag cgtggttcca tgatgctgtc tgcaaatatc
     6601 gatcaccatt tgcggcatat tataatcttc caatattttg gcaccatctg tcacatgtcc
     6661 aaagataata tgtttactgt cttctggcaa taagaaattg tgcggatttt ccgctcctga
     6721 aggcaagttc tcaacgaaaa agctggcgtg tttgatcttg ccgatatcat gataatagca
     6781 agctactcga gttagtaaag aacgtccacc gatttctgct acagcattag cacttagatt
     6841 ggctaccatc atgctatggt gatacgtgcc tggtgcttct tccagcaact gtttcaataa
     6901 aggatgatta ggattgctga gttcatttaa tacgatcaca ctatcatcat tgaccatcaa
     6961 ttcaatatag gggtgcagac ctatcgtcag caagaatgag aacagtgcac ctgtgaatcc
     7021 gcatactagc gttaaccaag tcgttgcatc cccaaagctc attccttggt agataatcaa
     7081 aatgacatcc atcatcacag gaaaaataat gacccacata gcagcggata acccttgttc
     7141 tgagatccgt ttgcgtctga cgatcgttgc catgagacca gaaaatacat aggacatcaa
     7201 aataactgtc agaaagttcg tgccaatcga atcataaaaa atgaaaagtg cggatactgc
     7261 ttgaaaaata ccagccatga tccctgctct tcgattcaag aaaaagctta aaacaagtgg
     7321 agcaaaagcc gcggggtaaa acagcggaat ataagcagct tgttctgttt ggaagagttg
     7381 gaaaaatttc attagtacga cgcttactga cattgccgtc acgtagaaaa gtacgtattt
     7441 tgttctttct cctgcatttt ggaattgaag tgaattataa atcagtacac cgatttgaag
     7501 caaaacagct aaaaccatgg caatgagtgg gaaaatagaa gtcgtttgat tcgtcaatcc
     7561 tagtaattcc aattttttca tcgctgaagc atcaatttga tttccttcgc gaacaataat
     7621 ttctccttgg aagatcatca ctggttgtac agcttctcgt gcagattgtt ttaattcttc
     7681 tgtctttttc tcattcaata catcattaac tacaatgcct tgatccacta agtagcgaat
     7741 catttgttgc tgctctgttg tgacattcaa atattgaatc tgatcttctg cttcttgttt
     7801 aaaagcttcc aattcgcttt cacgaacttg tttactcatc tgttcgtcaa tgagcttcaa
     7861 actttcatcc ctaaccgtgt gaatttccgt ttcagtcatt tcaaatatcg tcgaataaaa
     7921 attgttcggc agtttttggt aaaacgcaac gttttctgca ttcacgtttt caaaattttt
     7981 cttcagtgta gcaatccgct cttctgtagt aggtttcggg atattttcat cttccttcgc
     8041 cttatctacc ttgtcattat aagatttgtt gatggtatca ttcgtcttat cgatcaattc
     8101 gaataattgc ttgatccgat tgttctggtc atctgccaga tctttttgat aagtatattc
     8161 tggagtcact gcttctgctg ccagttttct tctttgttcg gtttcttccg tgttctcaat
     8221 cgttttattc gcacggatac tctcttctgc tacttggcct tctctgaaat cattggcctt
     8281 ttgtttgaca ctactaaatg taacgccgca gacaatgata aaaaacaaga acagaaggaa
     8341 aggcaatgct gcctttccga gtttttcttg tagtttctgc agtgttttat tcaacatgtg
     8401 cttatcctcc taagcaaagc ttattcttga tgaagatccg ccttttcata agctttgatg
     8461 atttcggcaa ctacagggtg ccgaacaacg tcacttgctt caaaattaac aaaatcgatt
     8521 tttttgattt gctttaatgt tcgttctgca tgaacaagac cacttgtcgt cccacgaggc
     8581 aagtctatct gacttgtatc cccattgaca atcatttttg attgataacc gagacgggtc
     8641 aagaacattt tcatctgagc gactgtcgtg ttttgtgctt cgtccaagat aacgaatgca
     8701 tcatccaatg tccgcccccg catgtaagct aaaggtgcga tttcgatcac gcctcgttcc
     8761 atcaatcggt tcgtatgatc caatccaaaa atttggtata aagcatcata aacaggccgc
     8821 agataaggat ccactttttc tttcagatcc ccgggtagaa agcctaggtt ttcccctgcc
     8881 tccacagcgg gccgcgtcaa aatgattttt tgcacttcgc ctttttttag ggcagcaatt
     8941 gctaatacta cggcaagaaa cgttttccct gttccggctg gccctacacc aaatacgatg
     9001 tcatgtttgg ctaccgattc aacgtatttc ttttgaccgc tgttttttac ccgtataggt
     9061 tttccattac ggtctttgac gatctcttcc tcatacatct caacaaaata atctaatgta
     9121 cctttttgcc ccatcttcaa tgccgtcaca acatctggcg tacttacgtg gattcctcgt
     9181 ttgatcaatt cctgtaatgt ccgaatgact gaggcagcca aggaaatctg ggattgctca
     9241 ccaatgatct gaatgatttc tccacgtgta tggatggttg tttccgttgt ttcctcgatc
     9301 agttttatat gcttgtcgtg tgcacctaaa agcatgctga catcatcagc agctgtcaat
     9361 ctaatctcta aagaactaga ctcttccgtc aaaaatagac atcccctttt tttcgattca
     9421 ttctttccta ttttaccata ctcagaagaa actatatagc aaataagaaa aggttgtaaa
     9481 agagtcgttt aaccatacgt accaagaaac gttaaaaaat agccgtttcc attcgctaaa
     9541 ggtctcgttt tgataaaaag aagctgagaa tgtaagttat atgaaagtgg agaacaaaat
     9601 attccgtttc attcagcagg tattctctag gtatttttct tgtttcaaaa aaagaaaaca
     9661 aaatagttaa aatattcttg catttcaaaa aaaacaggtg tattattacg tcaacaaata
     9721 aacatacagc aaagcgtcga ggaaaacagt aggctattca acgatttatt cccagtgagt
     9781 catgtaagtg agaaatgaca ataaatcgaa tagacgaaca tcatttccga gtcaatgtcg
     9841 aaaacgtaga tcattgtggc tgcctccatt atcaggctag cggataactt tatcaaaaag
     9901 cgtccgtgaa tgaagaagct tttttaagcg aaaaaaggtg gtaccgttct agaatctaga
     9961 gcccttttcc caagtggaaa agggcttttt ttatatttta aggtggtggt tttcatggaa
    10021 tctgatatta gtggcattac taattgctgc aacgtttata tcgtcaatca aaatgataat
    10081 tattggagga aaaatttatg gaagaaacaa aaacttattc atggaaacaa ctattgatgg
    10141 tcagttcact tatcttcggg atgttcttcg ggtcaggtaa tctaatcttt cctgtccact
    10201 taggtcaaat ggctggcgca aactgggtca gtgctggcgt aggatttgcc atctcgggcg
    10261 ctcttttccc attactagct atcttagctg tagtagtgac aaaaagtgat ggtctgtatg
    10321 acttagctaa acctgtcgga aaatggtatg ctgccttgtt cttaatcctt gttcatttga
    10381 cgatcggacc atttttcggt acaccacgga cagctgctac tgcttttgaa atggctgcta
    10441 aaccattttt gcctgaaaaa tataattcat taggtatgtt tttattctct gctcttttct
    10501 ttttacttgc ttatttcctt actgtccatc ccacacggtt ggtcaaatac gtcggaaagt
    10561 atctaaactc cttgtttctt gcattgttag caatcatttt tgtcgtttct ttattccacc
    10621 caatgggaaa tctaaatcaa tctgctgcag atacttacca aacaaacgct ttaacaaatg
    10681 gtgtcctaga aggttataac acggttgatg cagtagcctt gcttgcctta agtgtcactt
    10741 ttgtccatgc agtccgtgac cttggtttca aagataaaaa agtcacagaa ttaacagcaa
    10801 aagctggtag cctgagcgtt gcgctagaag tcatgatcta ttttggtctt gttcttctcg
    10861 gtgcgatgag tttgaataag atgtctttat cagaaaatgg cggaactgca ctttctcaga
    10921 tcactaattt ttatttaggt aacttcggcg ctagcttctt aggagtgatg gtcacattag
    10981 gtgtcttcac taccgctatg ggactcgttg tttcttttgc gcaagatttc cataaactgt
    11041 tccctaaagt cagttatatg acatggctgc ggttaacgac tttcgtctct tttgtcgttg
    11101 caaatgctgg tttagacaat atcatccaat ggtctttacc agtattgatg cttttgtatc
    11161 cattgtcgct tgcgttgatc ttgctttcct taacagcaaa atttttccaa aagacaccat
    11221 ttgtctatca agtaacgatg ttgtttgctg ctgttcctgc cgtattggat atgctggcaa
    11281 attcgcctgc tttagtgagt caacaaagag ttgtagcaag catgctcgaa ttttatcatc
    11341 atcacgttcc ttttgctgca ttaggacttg gctggatggt cccaacgtta cttggttacg
    11401 ctggcagcct actgttttat tacgcctatc gcctatcagg gtataaacaa gaagcaaatg
    11461 aactaccaga agaataacca aatcaaacac aatttaacat aagaaatatt aaacaggaaa
    11521 aaaccagcta gatgaaagaa tcctattctt tcgtctagct atttttttgt tctctttttt
    11581 acgcgcacaa aaaaactgcg gacaatgatt gtccgcagtt taaatgataa aattaagatt
    11641 gtaatagttc tttcacaatg gcatttactt ggtttccatc tgcttttcct ttgactttag
    11701 gcatgatcgc acccatgact ttgccaaatt ctttcattga tgaagcacca gtttgtgtga
    11761 ttgcttcttg aacaagttgt cgaatttctt cgtcggaaag ttgttccggc atgtattttt
    11821 cgactatcac aatttcactt tttacttttt cagctaaatc gtcacgtcct gcttcttcaa
    11881 actcatgcaa agaatcacgt cgctgtttca tttcgcgaga caacaccgtt aattcttctt
    11941 cttctgtcaa gtcgtgacct tctttaatct gctcgttttg tatagatgac ttgatcattc
    12001 ggatgacttg taaagattcc ttatctttcg ctttcatcgc tgttttcatg tcatcgttta
    12061 atctacttag tagtgacaca ctaccaactc caattcatca cagaactaga attttttgcg
    12121 ttttctagct gcttcagatt ttttcttgcg tcttacactt ggtttttcgt agaattcacg
    12181 tttacgagat tcttgtaaag tacccgcttt tgacacggaa cgtttgaagc gacgaagagc
    12241 atcgtcaagt gattcgtttt tgcgaacgac tgtttttgac atgttaattc cctccctccg
    12301 agcttaaaaa gtaaccgtaa ttgttattga aatatatctc aaaaaacaag actgtcctgt
    12361 aaatattata tctaatgctg ttttgaccgt caaggaaatc ttgctttttt ttaaaattat
    12421 caaatgttat tttatttaaa tccttattga caattttcac atcttccgaa aatctcgaat
    12481 ctatgccctt cgatcgtaca tccaccgagc tgttcttcga aataattcat tggacacata
    12541 tggatttctt tcgttgttcc gcacaccgta cagataaagt gatggtgatg gccgatttcc
    12601 ccttgacagc aatgaaaacg aaacttcatc tctccattta aatcggtttc ttccagtaaa
    12661 tcgatttctg caaaatcatg gagatttcga taaatcgtat cgtaacttac tcctttgaat
    12721 tcctgattca tatagtcaaa aacttcttta gcggatacgt agcgattgcg cttcactaaa
    12781 taagttaaca atgcttctct ttttttagta tatttaaaac cgttattttt caatgtttct
    12841 agtgattgtt caatcaatgt ctgttttttc atgacttatt cctccaaatc aaaatgatta
    12901 cgaagtacac attctagagt atggtataat aaacataaaa aaaatgcaag tgaggtttta
    12961 tccatgtctt ccgattctac agaaatcgtt actttttttg tcatctctga ctctgctgga
    13021 gaaactgcga caaaattagc gcaagccact atggcccaat atcctaccgt tgagttcaat
    13081 ttattccgca gaacatttgt gacagatgag aaaacattga tgcaggcact gcaagatgca
    13141 ctaaaagaaa aagcccttgt cctgcacaca ttgatcaatc aagaattgat cgagatgacg
    13201 cgttcttttt gtgaagaaca tcaattattt agttttgatg tgatgactcc gccgatatca
    13261 gaaatcgagc gcttaactgg tatcaagccc attcggcaac ctggcgccct tcatttattg
    13321 aatgagaact atttcaaacg gatcaaagca atggaatttg ctgtaaaata cgatgacggg
    13381 aaagatcctc gtgggtttct agaagcagat gtcgttttgc taggcgtatc cagaacttcc
    13441 aaaacgcctt tgagcttgtt tttagctaac aaaaatttaa aggttgccaa tctacctttg
    13501 atcccacaag cacatattcc caaacagctt tgggagatcg atcctaaaaa aatcgtggga
    13561 ctgacgaata acccagatat tttaaacaat atccgtaaag aacggatgcg ctcttacgga
    13621 ttaaatcctg atactgctta ttccgatatc gaaaaaatac gagctgaact cgattttgcg
    13681 aatgatcttt atgaaaaatt gggctgcgta gtaatcaatg tcgcttcttt atctatcgaa
    13741 gaaacagctt cacttatttt gaatgcttta gacctagaag atcatagtta ttatggaact
    13801 gaaaccaccg aagaaacaga ctaacagttt ttcttgtctt tttcttaaaa aaactggact
    13861 gtgtcagcga agaagattcg ttgatacagt ccagtttgtc tctatttgca cctatcgtta
    13921 ttgatgttcc acatgtttga gcgtctggtt caaaatatcc agtacgtgag aatcatcaag
    13981 actatacaaa atcgcttttc cctcacgatg ggacttgacg acatggtttt ctcttaataa
    14041 acgcagttga tgcgatacag cagattgttc catttctact gcttgggtga ttgctgtcac
    14101 attttgttcc ccgtctttca gatacagcag aattttgaga cgagtggggt cactcaacat
    14161 tttgaatagt tggctgactt tctctacttt tgttttttgc gtgtctggca atccattcac
    14221 ctccaacttg aaaaaagact gacttctaaa gtttaccact ttttccatca gtctcaagtt
    14281 tttttattct tctgtcattt ttttgataat cgcttcataa gcggctactt tttcagctgc
    14341 gtcttctgaa gagtttcctt ttacaccgat ataaaatttg attttcggct cagttcctga
    14401 aggacggata gcgatccagc cattttcttc cagcgtatac ttcaatacgt cagatggcgg
    14461 tgtcgttagt ttttctacgt ttccttcacg gtctgttctc gtcaactctt tgaagtcttc
    14521 tgtttggatg acttttgctc cggcaaattc tgtcggtgct tcttcacgga atttttccat
    14581 caaagcagag atttttgcag aaccttcttg tccgctcatt gtcacagaaa tcgttttttc
    14641 tgcgtagtag ccatatgttt caaaaatcgt ttgcaagccg tcatacaacg ttttgccttg
    14701 ttttttatag taagctgcta cttctgctaa taagagcaat gcttgaatcg cgtctttgtc
    14761 gcgaacgaaa ggtttaacta aataaccgta actttcttcg aatccgaaca taaaagtatg
    14821 ggaatgatct tcttcaaatt gttgaatttt ttcagcgatg aatttgaatc ctgtcaatac
    14881 attgaacatg gtagccccat agtgttccgc aattgctgta ggcaattcgc tagaaacgat
    14941 tgatttcaat accgctgcat tttcaggtaa agtgcctcgc gattggtgtg cttccaaaat
    15001 atattgagca aacagggctc cgatttggtt ccctgtcaat acttcatagg agccatttgg
    15061 caaacgaacg gctgcgccta aacggtctgc atcaggatct gttgcaatca gtaagtcagc
    15121 gccttctttc tcgccaagtc gcatcgcata ctcgaaagca gagtgttctt ctggattcgg
    15181 tgatttgact gtagtgaaat ccgggtctgc gacagcttgt tctggaacta gtacgaattg
    15241 ttcgaatcca gcttgtttca acgcacgttc gcccaacatt tttcctgttc cgtgaagtgg
    15301 tgtgtacaca agtttcaaat ctttacccat tgtttccacc aattcatgat caatcgttac
    15361 tgctttgatt tcttctagat aagcggcatc cacttcttct ccgatgatcg tgatcaatcc
    15421 gctatgttca gcttcgtcgt cagataatac ttcaactttt aacggattgc tgacttcacg
    15481 gacatatttc gtcaatgcat ctgcgtctgc aggaggcatt tgtcccccat cttctccgta
    15541 gactttatat ccgttgtaag ctgctggatt gtgggatgca gttaccatga ttcccgcaaa
    15601 cgtcttcaaa tgacggactg caaaagaaag ctctggtgtt ggacgtaaac tttcgaatac
    15661 ataagaaggg atattgtgat gtgctaatgt tttggctgct tccatggcaa attctggtga
    15721 ttgatggcgt gagtcataag cgatagcgac acctcggcgt tttgtttctt caccttgtgt
    15781 atccatgaaa cgtgcaagtc cttctgttgc ttgtcttacc gtatagatgt tcattcgatt
    15841 gatcccaggt cctaagattc cccgcatgcc tgcagtaccg aattccaacg gtgcataaaa
    15901 agcatcttca agtttttcag gatcttcacc cagatcaatt aattgttttt ttaagttttc
    15961 ttctaaattt tcttcatgta cccattgttc atacacttgt tcccaagaca tgaaaataac
    16021 acctctttct gaaatatggt tcttaataag tatagcattc acttattaga tatgaaagta
    16081 cgaggtctaa aaaacgctta aatcaagaaa ggattctttt atcaagcacg gttgcaagta
    16141 ttcgcataaa tcggattcag tagatttcac gcgaaaaaaa agagtaaaag aacaattgaa
    16201 accgttcttc cactcttttt aaacttatgc agttgtttct tctaaagatt ccaagtattt
    16261 atacacttgt tgtccagcga tcccaccatc accaacagca gtagtgattt gtcgcaaatc
    16321 tttttcacga acatctccga tggcaaaaac gcctggtata ttcgtcttca tttcgttgtc
    16381 tgttggaatc cagccagctt cgttcgtcaa ccctgcttct ctaaatggtg cagttaatgg
    16441 atccagtcca acatagatga atactccgtc tgctgccaaa tcaaactcat tgccggtttt
    16501 gacattacgt gctttcacac ctgtcactac catttcattc ccaacgattt cgtcaacgac
    16561 tgtatcccac ataaaggaaa ttttttcatt cgcaaatgca cggtcttgga tgatcttttg
    16621 tgcacgtaat tcatctctac ggtggacgat gactacttca gaagcaaatt gtgtcaaata
    16681 gatggcttct tctactgctg agtctcctcc tccgatcact actagacgtt tgttacggaa
    16741 aaacgcacca tcacaaactg cacagtaaga aacgccacgt ccagcaaatt cttcctcgcc
    16801 aggtacgcct aatttacggt ggatacagcc tgttgcgata atgactgttt ttccttcgta
    16861 agttttatct tcacaaataa cttctttgtg gctgccgtga tccttgatat cttgcacgat
    16921 tccataagca ttttctgtcc caaatcttga aacaccttca tacatcttat aagctagatc
    16981 aggacccatg attgaatcga aaccaggata gttttcgatt tctgccgtat tgttcatttg
    17041 gccgccaggg gcgccttgtt cgatcatcaa tacggataag tttgaacgtg aagcatataa
    17101 agcagcagtc atcccagcag gacctgcgcc aataacgatt acatcataca tatgtagatt
    17161 ctcctttact taatcaagta agcatacttt acagccaatg cgaaaaaaag tctattgttc
    17221 tgcttgcgtt tctcgaatag ctttattata ctcgaaaaat ccgctgaaaa acaatagact
    17281 tttacaaatc attgatcaat cattcgaaag attttctcca tttgaatcaa ttactttttt
    17341 ataccagttg aatgatttct tcttcgaacg ggctaatgtg ccatttcctt cgttgtcacg
    17401 atcgacataa atgaatccgt accttttctt catctcacct gtaccagctg atactaaatc
    17461 aatacatccc caagtcgtat agcctaatag atcgactcca tctaattcta ctgtatcttt
    17521 cattgcctgg atatgtgctg ccagatagtc gattcgatag tcatcttcca cataaccatt
    17581 ctcatcgggc gtatcgactg cacctaagcc gttttctaca ataaatagtg gtttttgata
    17641 acgatcataa atatcattca tcgttattct caagcctaat ggatcaattt gccaacccca
    17701 ttcacttgct tccagatatg gatttttgac agaagcaaag atatttcctg cagtctgttc
    17761 caagagttcg ttgtcagtag tcgaaactcg agaagaataa taagaaaagg agataaaatc
    17821 aacagtatgt tctttcagca atgcggcatc cccaggttcc attttgatct gaatgccatt
    17881 tcgttccatt tctttcaatg cataagcggg atattctcct cgtgactgga catcaatgaa
    17941 gaaataattt tctcgatctg attttctaga agcccaaaca tcttcaggat tgcaagtgta
    18001 tggataatat gctcctgctg ccagcataca gcctactttg ttctccggat ctacttcatg
    18061 agcaatcttc gttgcgatgg cacttgcaac caactcatga tgtgccgctt gatatttgat
    18121 ttgttcttca ttttctcctt cttcaaaata cagccctgcg cccatgaatg gtgcatgaag
    18181 gatcatattg atctcattga atgtcaacca atatttgaca agtcctttat agcgattgaa
    18241 gatgactcgg cacagatttt cataaaaacc aacgagttct ctagaacgcc acgcaccata
    18301 ttctttgatc aaatgcatcg gacaatcaaa atgtgtaatc gtcacaagcg gctcgatccc
    18361 gtacttttga cattctttaa agacatcctc ataaaatttc aaaccagatt catttggctc
    18421 attttcatcg cccattggaa aaatacgact ccaagcaatg gacatccgat aagtcttgaa
    18481 ccccatttct gcaaacaaag caatatcttc cttataatga tgatacatat cgatcgcaat
    18541 ttttgctgga taaaaatgat ctttatcaaa ttcaaacatt ttcttttttc cagtaatgac
    18601 aggaaaacgg tcttctccga ttggcacaac atctacgttc gctaacccac gtccgccttc
    18661 atcataacca ccctcgcatt gattggctgc tgtagcaccg ccccataaaa aatcttttct
    18721 gaatcccatc atacttcctc cattacatgt gtattgcttg acaattctct tgccagttca
    18781 tcttggactt cgattccttc tgcttttaac cggctgattg tttccttgaa ttgcggtaaa
    18841 tactgctgat gtgcaataaa caactcatcc atgatccgtt ttgctgtctt tcctgatcgt
    18901 ataagcggat tgatcgtaaa cgcttgtaaa gctgttccat aatcaccagt cacggctgct
    18961 tcgatcgtca gcaattccat attcttcatt acttgcaacc agcctctttc agcagcagga
    19021 agggaaccaa acgctacatt tcgtgcgcct tgtgcgccaa cataggcagt gacttctact
    19081 acacaatctg ccggcagatc aggaacagct ccatcattct ttgtagacac cacgatctgt
    19141 gtttctttat ttccatagat cgaagcaatc gtttcacagg cagcatctga ataataagca
    19201 ccacctcgtt tttgcaattg ttcaggttta tgatccaatt ctggatcttt gtataattca
    19261 aacagctctg cttctgtttc tttcacttgt tgcgctcttg taccaatcgt ttgatattct
    19321 tctaaagcat gttccaacat ttcttcttca cggtagtaat agcggtggta gccacaaggg
    19381 atcatgttca tttgttttaa ttgttctttg aaaaacggga tgtcaaagat atttttcggc
    19441 aatccattgt cttcatcgta taaacgatca atcaaatctg ctgtgcggtc atttccttta
    19501 ttatctgcta ctttgtgcca atggaagtga tttagaccgg caaacttgta gatcagctct
    19561 tcttttttct tgccaagcat tgctggttct gtcatcgtcg ccattactgg cacattgcat
    19621 aatccgatca ctttatccca tttgccatat ctgataactg cttctgtcac catcccgctt
    19681 gggttcgcga aattgatcag ccaagcattt ggacataaac gcttcatgtc ttcaacgatt
    19741 gccaaaacca ctggaacggt acgaaaagct ttgaatatcc cgccagcacc atttgtttct
    19801 tgcccaatca tcccataata actagggatc cgctcgtctt tgattcgtgc atctaattga
    19861 ccgacacgaa actgagtcgt aacaaaatcc gcatctttca atgcttcttc ccgatccaaa
    19921 gttgcataga tcttgacttc atacggagaa gcatcccaca tacgctgaga catctgactg
    19981 acgatctcaa gcttttcttt tccttcttct acatctacga gccagatttc tcgtatcggt
    20041 aattcctcat atcttttgat aaatccttcc atcaactcgg gggtgtaact gctgcctcca
    20101 ccaatcgtag cgattttgat tccttttgcc atgttctcac tcctttgctt tttacacttt
    20161 tattatcttt tttgtaaaca aaacaaacaa tagaaattcg tttctttcca gtaacattta
    20221 caaaagttag tgttttctac tggatttttg taaatatctt gtcaaaaaat gactttatta
    20281 agctatataa cataaatccg tttacaaatc attttcaaaa aagttataat ttacgtaaaa
    20341 aaactagaag aaggtatcta catgttatta cgtgaaaaaa tcaaagaaat ctccttttct
    20401 ccttcagaaa atgaagtgat tcagtttctc ttaagcgatt ctccagcact tggcgaaatg
    20461 accatccaag agattgctga gcgatgttat gtccacccgt ccacactcat ccgtgtagca
    20521 aaaaaaatgg gttttgatgg ctgggtcagt tttcgtaatg cttttttagc tgaaacagcc
    20581 tatcttgaag ggaattttca agacgtagat gcgaatcttc ctttttccac cacagacggt
    20641 attatgacga tcgcaaataa aatggcttct ttagaacaga tgacgattca agatacactt
    20701 tctctcattc atcatgacca actgcaaaaa gccaaacaac taatgcttca agcaaaaaaa
    20761 atcgtgattt tcgccagtaa ctcgaatact ttgatttctc aagatttcat gctgaaaatg
    20821 aagcgcatca aaatggatgt cacgattgtc accacttttg gtgaatcgtt ctatgaagcg
    20881 tacaattgca cgagtgatat gtgcgggatt ctgatttctt atacgggtga aaacaatgtg
    20941 atccttcgta ccgcagaaat attgaaacaa aatcatgttc caattttagc gttgaccagt
    21001 atcggtgatt caacgctatc ctcaatgagt acagccgctt tgcgacttac gacacgcgaa
    21061 cgactgtatt caaaaattgg taattttacg atcaatacat cgatttgtta tcttctagac
    21121 gtgttatata gttgtgtttt tgcagaaaat tatcaacaga atttagtcca tctaatttct
    21181 gtaggacaaa aatttgataa acgccctatc agctcagcca tcatgaaaga gcctttcaat
    21241 tttgaaaata aagaagtgga ctaaaaagaa tagttaagtc tattccattc tttcacctaa
    21301 ccgttcaggt cagtgcggtg ataagtgcat cgtctggccc gaccgtctcc ccttgatttt
    21361 ccaaaatatc caaataatcc atgtaattgg tgacgattac aggtgtttct aaactgtatc
    21421 cttccttttc gattttcttt ttgtcgaagc ggagcaacag atcgccttgt ttcactcttt
    21481 ctccttgctg caccaagctt tcaaaatatt tcccttccaa ctgaacagta tccaatccga
    21541 catgaatcag caattccaga cctgtttcag aaaccattcc gattgcatgt ttcgtgggga
    21601 acagggtcat gactgttccg tcaaaagggg cacgtacttc tcctttttca gggtagatca
    21661 agattcctcg acctagtgtt ccttgtgcaa aagctgcatc ctttgctgct ttcagtggag
    21721 cgatttgtcc ttgtatcggt gtttttacaa tttctttacc tgatcttttc tgtctgtcat
    21781 ctgtttcacc tgcttcctcc ttccaaaaga aaaaagtcag cgcaaaggat accaccatcg
    21841 caatggcgat cccaatcaat acattaatca catctgttaa atcaccatct ggtctaatat
    21901 agttgggaat tgtaaagatg cctagtccac cttgattcca agaagttaaa ttcattaacc
    21961 cactatacgc gccccctaac gcaccaccga cacaagtaaa aataaatatt tttttacgtg
    22021 gtaatgtaaa cccataaata gctggctctg taatccccac aataccagaa acaatggctg
    22081 gtattgcaat tgctttgtct tttggatttc taagcttaaa gtagagtgcg gtaagtgctg
    22141 cagtttgtcc aaaagacact actgcaacag gtgtcataat attttgccaa ccattcacag
    22201 aaaattgcat caatcccatt ggtacgattg cccaatggag accaaacatc accagaattt
    22261 gccagaagaa gcctacgatt gctccataaa taactggcga aagtgctaaa agtgtttcaa
    22321 atccagccat taaaatatca gttgcgaatg tgaatactgg tccaacgagt aaaaaaccta
    22381 aaggcattgt aataagaacg gtgaagaatg gaacgaagaa attcgcgatc atctcaggta
    22441 ctaacttttt aattggtttc tgaatttggc tagctaacaa cacaataaag ataatgggaa
    22501 tgacagaaga agcgtagtta cgcgcaatcc agggaatgcc gaatactgtt tgataaatag
    22561 gtgcttcaaa aatagtgcca gaaaatatgg tcgttagtgg tgcttctgct gcaccactca
    22621 aagcttccag cgacaagcta ggattcatta atgctgcgcc aagcaacatc cctacgaatt
    22681 catttacttt aaatttacgg gcagctgtta aaccgataat aacaggcata aattggaata
    22741 cagaatcacc gatattatca aacataacaa atgtgccaga accgcggtca aacgcgccta
    22801 agaagacaag gaatgctgtc agtccacgaa tcatcccgcc agcagctaag atagcaagga
    22861 atggttggaa acagcccgat attccatcaa taagcatatc aaacggtctc attttttctg
    22921 ttgaaacagc ctcatcaaaa gtaaggtttc ctgcttttat aacctcttca aacacgactg
    22981 gcacatgatt cccgatgaca acttgatact gccctcccgc tcgcattacc gttacaatac
    23041 cgtccatatt tttcaagatc tcatcatttg ctttttcttc gttttttaat ttgaaacgaa
    23101 gccttgtgat acaatgtgtc aaaccattga tattttcctt cccaccgaca ttttctacga
    23161 tttttctcgc taattcttgg tattttccca tttctattcc ctccaatata ttagtggaag
    23221 gtttagccaa ctgaatgtca ctacccttat ttcatacgtt cttgttaatt tatcctcctt
    23281 tttttattta tttacaacga ttctcatgca ctaaacgcgc agtatggatc gttaaataca
    23341 ttttttcgtc cgtggacatc tggtagttga acgtctcggc taaaaagtct gctattttct
    23401 caacgcaacc aaacgtatcc ttatattttt tttgaataac ctgaaacaat tcctcagaaa
    23461 cttctcccac ttctgatttt tctccatcta ggacgcgtgt cagaaaaaaa cgtaagtgtg
    23521 tgacgaatcg aaaaaaatgg actgaatctt catcaaatgt aatcttatag taatagcgga
    23581 cgatatttag aatatcctgt attgttatag taaacaggta ggtattgttt tgttggcttt
    23641 gaatgccttg tgcattgact aaatgcaatg cgataaatcc ggcttcatct tctgataact
    23701 tgatgtggta tttatccgag attttcttca ctgttttttt cccgatggca tactctttag
    23761 gaaatagttg tttgatatca tacagcaata catttggtac aaatattccc attttgtgac
    23821 gttcgatagc cgtatacaaa tgatccgtca atgcaatata cacagaatca ttcaactgga
    23881 cttgtagagc ctcttttgta tattcaatga tttcacaaga aatttctaag tactcaagag
    23941 gaatcgcttg tgccaattct tggaactgtt gtgaaagccc gtcatttgtc aactgaaatt
    24001 ttttgtcgat gcatgagtca aaaatcgggt cacccacttt ctttttgaat gccaaacctt
    24061 ttcccatcac gatcatttcc tgatttcttt catccctggt tattacgacg ttgttattca
    24121 gtactttctc aatcagcatg ttttgctcct ttccatgata tatagggctg tattcaaaac
    24181 aaaaagaaaa acccaaaaag ccgtccatgg aaccatggga gactcatcag gtttagcttg
    24241 catcaggcaa tcactatcca agttgtatcg ttttctttat attatcgaaa gcaaacgctt
    24301 tcttagtggc tattaggcac ttttgactag ctgtcgtcgt cttttttaaa tcgggtgact
    24361 acgccactaa cgctcacaga tgaagaatct gggaagctct gttttatttt cactctagcg
    24421 cttaggacaa aatgctacta tgctactgtc acaacctctt tttacacacc actgctttgc
    24481 agtttgtaca tatctgcata caaaccattt tgcgctagaa gtgtttcatg attcccacgc
    24541 tctactatat gacctttgtc taaaactaaa atcaattctg catcccgaat cgtggacagc
    24601 cgatgggcaa tcgcaatcgt tgtccgtcct tgtcgcattt ttgccaatcc ttcttggatc
    24661 aatccttcgg tttcagtatc gatatttgct gttgcttcat ccaatactag tattttagga
    24721 tctgtcacga ttgttcgcgc aaaagtgatc agctgtcgtt gtccactaga atagctggca
    24781 ccgtgttcga tcacttttga gtgatatcct tttggtaatt catcgatgaa cttgtttgct
    24841 tgaacaaact cagctgcatg gcgaatctgt tcgtctgtga tagctggatt catcatccgg
    24901 atatttcctg caatatctcc ataaaacaag aaggcatctt gcaagaccaa tcccattttc
    24961 ttacgtaact cttccattgg atattctcga atgtcatgat catcaatcaa gatttggcct
    25021 tcataaaatt cgtaaaaccg catcaatacg ttgatgatcg agctttttcc gcttccggtg
    25081 tgccccacta atgcaacggt ctcacccgga ttagcgacaa aagagatatt gttcaataca
    25141 ttgtgctctc catcataaga aaagcttaca ttgcgaaact cgatcttacc tgaagtaatc
    25201 gcttgatctg ctccaggatt ttgttgcggt gcatattctt ctgtatccat gattttcaaa
    25261 atacgactac cggcaaccat cccatctgta aaggtgctca aaaagtccat catttgagac
    25321 attggattaa aaaatccctg gacataggtc gtaaatgcat agatcagtcc aacttctacg
    25381 ggagaatgca acgcatcgat cccaaacaaa gtcaacgcca acgcaatcgc caatgcatag
    25441 agaaaattga tgatcggact taacagaatc gaattcgttt tgatcatcgc atagcgcgtg
    25501 tctaaatact cgttgttcac ttgttcaaat tctttagcta aacgagcttc ttggcgaaac
    25561 tgctggataa tacgcatgcc ggaaatcgat tcattcaatt tcgtgttcag ctgacttaat
    25621 ttttcccgca tatttcggta aatacgagaa ctgaatttct ggtaatacca gatgacgatt
    25681 cctaaaatcg gcaagaacac caaagtccac aaggcaattt ctccattgat ttgaaacatg
    25741 gcaacgaaag aagaaacgac tgcaaagatc ccagtcaata ccatcaaaaa gacataccaa
    25801 aattcaaata gcgtttctgt gtcattcgtc actcgagaaa cggtcgatcc tgccggtgtc
    25861 tggtcaaaat aacgcatacc tagtgtatgc agcttttcaa ataatcttac ccgaacatac
    25921 tgataggtct tcaatgaagc catggaatac aaataccatt ggaaaaacca aatgatactt
    25981 ttgactatga ctccaaacaa gtacacacca gcaaaaaaca aaataacttg tgtcgtagct
    26041 gactgtttag ccagatgatc atccataaaa gtttggatga tccttggcaa taggacattg
    26101 atcacagaca gggcaaacgc aaataagata gcaacgatga acgttcttct aaaaggtttg
    26161 gcgaacttca tcagtcgttt gatgatcttc ttctgttctt taagtggaat cgattttgtc
    26221 cattccgatt gataattttc atccatcagg cctcgcctcc ttctatcttc gcttctaatt
    26281 gctgtttttc ccacattcgt tcataccagc cacctaatgc aagcagctcg tggtgggttc
    26341 ctcgttcaat gattttccct tgatcgacaa caatgatttc ttttgcatgc atcacgctgc
    26401 ttaaacggtg agctgcaatg atggttgttt tctcttttct tatttctttt aaattatgca
    26461 agatagcttc ttctgttttg gcatctactg cagaaagtgc atcatctaag atcagcaatt
    26521 caggattttt gatcaatgct cgagcaatcg aaatcctttg tttctgtccg ccagataaag
    26581 agactccacg ttctccaact aaggtatcat aaccttttgg caattccttg atttcattgt
    26641 tgataaatgc taaagaagcc gcatgctcta cttcttcttc tgaaagtttt ggattagaaa
    26701 aacggatatt atctcgaatg gtcatcgaaa acaggaagtg atcctgaggc acataaccga
    26761 ttgatcctaa taaagcattc aatgaatatt ctttgatcga acgtccgcca aattggattt
    26821 tcccttgata atcatcgtat tcacgcatca gtaatttgat gattgtcgtt tttcccgcac
    26881 cagttttccc aacaatacct aatgtttctc cttcttggat cgcaaatttg atttgttcta
    26941 atgctggtga ttcttctttc ggatagttaa aagaggaaat agctacattc agctcgcctt
    27001 ttgccggggt ctgcactgca cccagttgtt ccacgatatg cgtcttttca tgcaaaagtt
    27061 cattaacgcg atcataagaa gcatttcctc tttccaatac gttgaacaat cggccaatcg
    27121 caaacatcgg ccaaacaagc ataccgatat aactaataaa tgagaccatc tgcccaatcg
    27181 agatcgtgcc ttccaagaca tactttccac ctaaaataat tgttgcaaca taagacagac
    27241 cgataatcaa ggtaatgaat ggatcaaata aggagtctaa aaaattgact cgtttgtttt
    27301 taataattgc atcttcaatc ttttcttcaa aatccgcgag atcttctttt tcttgtccaa
    27361 atgttttaat gactttgatc ccagtgatac tttcttgtgt cttatcattg atactagaaa
    27421 aagcttcttg cgaatcacga aaagcatcgt ggagttttga ccccagcact cgtgaagtaa
    27481 cggctagcaa aggcagagga atcagcgcaa tcagtgttaa gcgccaatca ataaacaaaa
    27541 tcatcgcaat gatcgttgtt ccgcccgtaa tcactgaatc tgcaaaggtc aagattccag
    27601 ctcctgctac attctggatt gcattcaaat cattcgttgc atgagccatc aaatctccag
    27661 tccgatattt ttggtaaaag agattgtcca tttctgtaaa atgtttgaat agctgctgcc
    27721 gaagttcttt ctccaaccga gctgcacttc cccaaatatt cgtccgccag atataacgaa
    27781 aaagatattg taagaaagct gcaaggatca atagtaagat ccaccaagaa atgatcttta
    27841 ttgataaatt cttatcggcg atctcatcaa tgatgatccc aatcactttt ggaggaacta
    27901 actgtaccaa ggccactgcg aatagggaga aaatcccaat taaataatgt ttcttttcct
    27961 gacgaaaaaa ccaacctaat tttctaaaaa tcgacatttt actcctcctt tgtctttatc
    28021 aagagcatag gattgtctgt ccttgtcttt tttgctgaat agtctgaata gtctaacaaa
    28081 ttacgaaata aaaaacggac tgaaaaaatc agcccgcaaa aagtctttgg gtcagaacct
    28141 attttttccc ttggttcttc atttgctgca tcatctggcg aattttcttc tcagatggtt
    28201 tttgccccat tgacatcatc attgatcgca acatgtcctc attaacggga ggattttttt
    28261 ctaaatagtc tttcatatat ttacgcgcta ggaaaaagcc acctactgcg ccgattagca
    28321 aagcaataat agcaataagt acgacaatac ctgttgatac catataaatc tctcctttct
    28381 atttccgaat tgctctcaat gatttatttt actaaagttt attgaaaaaa gaaaggcgta
    28441 ttctttagtt tcatgcaaaa aatagtggtt tttttagaga agaacaaaat ttctttcatt
    28501 aaccagcaag acacgccacg aaaaaaaatt aggcataccg aatctttttc tttaattttt
    28561 tctttctgag gtgtagaatg taatcgtaga taaataggaa aggtggtttt ttcttgaata
    28621 atcaatccaa caaacaattt cataagcatc gacttattga acatacaaat ggtttatctt
    28681 tagctgaagt aaatggaaca gtggatgtac caaagggaaa aagttttttt aaaacacttc
    28741 ttgcttactc tggaccaggt gcattagtag cagttggtta tatggatcca ggtaactggt
    28801 ccacttcgat cactggtgga caaaatttcc aatatttatt gatgtcagtt attttgatgt
    28861 ctagtctaat tgctatgctt ttacagtata tggcagcaaa attaggaatc gtgacccaaa
    28921 tggatttggc ccaagcaatc cgagcacgga caagtaagac attgggtgtc attttatgga
    28981 tcttgactga attagccatt atggcaacag acatcgcaga agtcattggt gcagcaatcg
    29041 ccctctactt actttttgat attccattgg taattgccgt atttatcact gtgttagatg
    29101 ttttcgtcct tttattgctg acaaaagtcg ggtttcgtaa aatcgaagct ttggttgtct
    29161 gcttgatttt cgtcatttta ttcgttttcg tctatcaagt tggcttgtct aaccctgatt
    29221 ggcacagttt atttgctggc tttatcccta acagtcatac attctcagac tcacctgtca
    29281 taggtggtga aactccgctg actggagcgt tagggattat tggtgcaaca gtcatgccgc
    29341 ataacttata tcttcattca gcagtttctc aaactcggaa gatcgatcac caagacgaag
    29401 aagatgtagc acaagcggta cgcttttcta cttgggactc taatatccag ttgtcaatgg
    29461 cttttttagt caatgcactg ctattgatca tgggtgtcgc tgtattcaaa accggttctg
    29521 tccaagatcc ttcctttttt ggtctgtatg aagctttatc tgatccatca gctatgagca
    29581 atggtatctt gattgctgta gcaaaatctg gtatcttgtc tactttattt gctgtagcac
    29641 ttcttgcttc tggccagaac tctactatta ctggtacgtt gaccggtcaa gtcatcatgg
    29701 aaggattcat ccatatgcgt atcccgattt ggctaagaag actagtcaca cgattgcttt
    29761 ctgtgattcc tgtattgatt tgtgttttgt ttacaagtac aaaaggaacg atcgaagaac
    29821 ataccgcttt gaataacttg atgaatgaat cccaagtttt tctggctttt gcattgccat
    29881 tctcgatgat tcctttactg atgatgacca atagccaagc ggagatgggg agccgattca
    29941 aaaattcatt tgttgtaaaa cttcttggct ggttctctgt gattagtctg acttacttga
    30001 acttacgagg attaccaggt caaatcgaaa gtttcttcgg cgagcaagcc acctcagctc
    30061 aactagcatt ggctgatcaa atcgcctatg taatcattgt tcttgtcctt ctcctgttgg
    30121 catggacgat tggtgattta tataaaggaa acaaaaaata tgaacagcgt ttgcaagaaa
    30181 cgatagaaga aatcgaatag aaactgattc ttttacacaa aggaggttgt gacagaagtg
    30241 ggcagcttca agaaataaga aggaatttaa gaaaattgct tttcaaattt ttgtgaaatt
    30301 tcggcttatt tccgaagaag ttacttctgg aacaccgtgt at
//
LOCUS       DASOUD010000026        30075 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.026, whole genome shotgun sequence.
ACCESSION   DASOUD010000026 DASOUD010000000
VERSION     DASOUD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.026"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..435
                     /locus_tag="U4A29_001052"
     CDS             <1..435
                     /locus_tag="U4A29_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEO0680189.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="U4A29_001053"
     tRNA            complement(759..842)
                     /locus_tag="U4A29_001053"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="U4A29_001054"
     CDS             1370..1870
                     /locus_tag="U4A29_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0680190.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="U4A29_001055"
     CDS             complement(2060..2404)
                     /locus_tag="U4A29_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO0680191.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="U4A29_001056"
     CDS             complement(2558..3478)
                     /locus_tag="U4A29_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO0680192.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="U4A29_001057"
     CDS             3665..4156
                     /locus_tag="U4A29_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEO0680193.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="U4A29_001058"
     CDS             complement(4180..6555)
                     /locus_tag="U4A29_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEO0680194.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="U4A29_001059"
     CDS             6881..7507
                     /locus_tag="U4A29_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO0680195.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="U4A29_001060"
     CDS             7530..8234
                     /locus_tag="U4A29_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO0680196.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="U4A29_001061"
     CDS             8426..9259
                     /locus_tag="U4A29_001061"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO0680197.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="U4A29_001062"
     CDS             9456..11384
                     /locus_tag="U4A29_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEO0680198.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="U4A29_001063"
     CDS             11522..11977
                     /locus_tag="U4A29_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEO0680199.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12447..12716
                     /locus_tag="U4A29_001064"
     CDS             12447..12716
                     /locus_tag="U4A29_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680200.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /locus_tag="U4A29_001065"
     CDS             13116..13370
                     /locus_tag="U4A29_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO0680201.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="U4A29_001066"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="U4A29_001066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO0680202.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..16816)
                     /locus_tag="U4A29_001067"
     CDS             complement(14543..16816)
                     /locus_tag="U4A29_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO0680203.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16809..17348)
                     /locus_tag="U4A29_001068"
     CDS             complement(16809..17348)
                     /locus_tag="U4A29_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEO0680204.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17484..17702)
                     /locus_tag="U4A29_001069"
     CDS             complement(17484..17702)
                     /locus_tag="U4A29_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEO0680205.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17699..17944)
                     /locus_tag="U4A29_001070"
     CDS             complement(17699..17944)
                     /locus_tag="U4A29_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO0680206.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(18060..18788)
                     /locus_tag="U4A29_001071"
     CDS             complement(18060..18788)
                     /locus_tag="U4A29_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO0680207.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(18947..19990)
                     /locus_tag="U4A29_001072"
     CDS             complement(18947..19990)
                     /locus_tag="U4A29_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO0680208.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(19980..20471)
                     /gene="coaD"
                     /locus_tag="U4A29_001073"
     CDS             complement(19980..20471)
                     /gene="coaD"
                     /locus_tag="U4A29_001073"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO0680209.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(20468..21028)
                     /gene="rsmD"
                     /locus_tag="U4A29_001074"
     CDS             complement(20468..21028)
                     /gene="rsmD"
                     /locus_tag="U4A29_001074"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO0680210.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(21147..21485)
                     /locus_tag="U4A29_001075"
     CDS             complement(21147..21485)
                     /locus_tag="U4A29_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEO0680211.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(21469..21906)
                     /locus_tag="U4A29_001076"
     CDS             complement(21469..21906)
                     /locus_tag="U4A29_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEO0680212.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(21896..23020)
                     /locus_tag="U4A29_001077"
     CDS             complement(21896..23020)
                     /locus_tag="U4A29_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680213.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(23041..26469)
                     /locus_tag="U4A29_001078"
     CDS             complement(23041..26469)
                     /locus_tag="U4A29_001078"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEO0680214.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(26495..27658)
                     /locus_tag="U4A29_001079"
     CDS             complement(26495..27658)
                     /locus_tag="U4A29_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO0680215.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(27661..27963)
                     /locus_tag="U4A29_001080"
     CDS             complement(27661..27963)
                     /locus_tag="U4A29_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEO0680216.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(28077..29849)
                     /gene="recQ"
                     /locus_tag="U4A29_001081"
     CDS             complement(28077..29849)
                     /gene="recQ"
                     /locus_tag="U4A29_001081"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEO0680217.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         8856 a         6100 c         5230 g         9889 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaata aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatagcccc ataataaaga attaatatat acacttgttt
    16201 tttgcggaaa ttcagattgg acaaaggaga taaaagatcc tctttttctc tgaagccagt
    16261 ttcgccagtg attttgcatg gtgatctgtt caatcttttg aatttggaac cgtccagaaa
    16321 ttctatggtt cctgaagtaa gcttcttggt caaaattata aagattccgc ttttttgctg
    16381 gagagatgaa ctcgccttca gcagtcaatt ctattccctt tcctcggaag ttaagccatg
    16441 ttttcaattc tgcttctgtg tttacttgat aagaaagctg gagcttttgt ttattctctg
    16501 ataaccttcc ttccattgtc aaaaaattac catttgcacg aatcgtatct gtaaatacag
    16561 taatttgcgc agatgttgtt ttttcctcta aaaatgaata ctcttgtagc ctataagaaa
    16621 tggataccgt tatgctacac aaaatacaca agtacgcgat aaaacgtcct tcttttagcg
    16681 aaaaccataa aaaaattccg gcaagaagca aacgataacc ccaattgtca gtgtaataca
    16741 acaaacagat aatcatcgac gtaattgccg gaaaaatcaa ccgatttcga acatgccttt
    16801 ctaaaacgct agacatgttt tcctgttaat tcattttgaa gttttgaaaa atgctgtgag
    16861 tctaaagcaa cttggtgtac agatacacct aattgttcga tcaacgcttg ggcataagga
    16921 tcatttcgat agtcatggag ataattgatc cctttcacac cagcttgcaa caaagccttt
    16981 gtacaggcca aacatggaaa atgtgtgaca tagatttctg aaccttccgt cgggatacca
    17041 agttttgcac actgtaggag tgcattcatc tctgcatgga tcgttcgcaa gcagtgtcca
    17101 tccacgacgt aacacccttc atctatgcag tggacatctc cactgactgc tccattatag
    17161 ccgccagcaa tgatcctttt ttctcgaacg agtgtcgctc ctacttctaa acgtgtacac
    17221 gtactacgta aagataacag tactgcctga gccataaagt actggtccca aggaatgcgt
    17281 tcattatgtg acttatcagt aactttttct ttattcgtat cgcatgcttt tgaagatttt
    17341 tctaacatgt tttatattcc tccgtttttt caagttgatt aaaaagtact cattgcttaa
    17401 acaaaatatt ctttaaatat aacgaatcat cctgtctgac aaaagatcct acccttcact
    17461 gccgaaatga agtccgtcat ccattagccc attataaaaa ttcttcgaat ccatttgttc
    17521 aagaaattcc gtataaaaat cgatacttca caatcatggc gttttgctac ttctgtgaga
    17581 acagagatat attctttgat caacttgtta ttccgatatt tttgttttgt ctcatcgact
    17641 gctggtggcg tgataaagaa agcatgatca gaactatagt cttttttcag ctgaactatc
    17701 atccactgca gattttttgg tacgtttctt tagaaagttg tttatgctct gctagatcat
    17761 tcgtcccaat gagaataaat actttatctg ctgtatagct aagtacatct ttggcaaagc
    17821 gttctcgtag agcttgactg ttattgccgc tgacagccgt attcataata tgaatcccag
    17881 atattttatt ttggagatag acattcagca ttggtttttt taatgtttct ttgcgaacaa
    17941 tcaagctatt tcctatccaa aaatactacc tcctcgtcct gccaagctct tagaattgga
    18001 ttattcttag tatccattag cgtccattta ttgctcgtgc cagtcgattt tgaatcattc
    18061 tatttaattg taacgaagtt ttttagtttt tcaactgtct tttcaccgat accagaaatc
    18121 tcctgcaggt cttccacttt ctggaaggat ccgttttctt ctctaaatcg aatgatttct
    18181 tgcgctttga ctgatccgat cccaggaatc tgctgaagtt ctgatggagt ggctgtattg
    18241 atatttattt tgggaggctc ggaatctgct gtaggctgtt ttccttccga ggatgccaac
    18301 tgatcaagat gttctggcag ctcttcacct acagcaggga tatataggac ttgctgatct
    18361 tctatcttcg cagaaaagtt gattcgatcg ctatccgctt cttctaataa tccacctgca
    18421 cgctttagta catcatagac ccgttcttct gaagaaaagg aatagatacc aggctccttc
    18481 acacttcctt tgatatcggt gtacatgacc tttggacgag catcctcttc tacatcgttt
    18541 ccttcagctg atggatcttc tccactggaa ctaaacgaag aaagtatttc ttttgcagca
    18601 gtctcttctg tttctctata aaatagcaaa tttgataata aaaaaccact tgcgatacaa
    18661 aagataagaa ctatcataga catccaaatg atcaatttgg gtgaagctag tattttcttg
    18721 ctgatttttt ctatcattgt tcttttttca aagaatgacc agtaaaaatc attcattact
    18781 cctttcattt aaaattgaag aaacagtgag ctctggcaaa atcgtccctt caacatctaa
    18841 ttacgtcaat tttttctttt gtttttattt ttatcgtgaa gatttttgac gaaaaagagg
    18901 ggttgtgaca gaactcgtgt cacaacccct ctttaattat ttagtttcat ttcattttct
    18961 ctagatagtc aagcgcttct tgtactgttt ttactggtac gattttcatt gaggaattca
    19021 attttttagc ggcagctttt gcttcttcgt agttggtttt tatttcagga tattctttct
    19081 taacatcgtc tgaaatcgta tcatctggtg caaagaaaat ctctacgcca gctttatccg
    19141 cacttgccac ttttttatcg attcctccga ttcggccaac ttctccttca ctgttgatcg
    19201 ttccagttcc tgcaatttgc ttaccatgtc gcaagttttt accagtgact tgttcataga
    19261 tttctaaagt gaacatcaat cccgctgatg gcccaccgat agagccagaa ttaatacgaa
    19321 catctgtatc tgattcgatc tcggtatggt cagtaagcgt gatacctatt cctgcctttt
    19381 tatctgttgg taactcgatc agatcaccag aagctgtttt ttcttcacca ttatgcaaat
    19441 aactgatttc gattgtttgt ccaacttttt ggctttttac ataatccata aatgcttctg
    19501 tactttgaaa gcttttcccg tctacttttg ttactgtatc tccaactgaa agcttgccaa
    19561 agaaattcga tgttttctct acactcatga catatacgcc tttgaattcc attttataag
    19621 gcttgtctgc aagttttaac gcttgttcga ttgctgcatt ttgggaagaa tccatataat
    19681 actgttgcat ttgattatat tcttcatttg tcgccccacc agtcaattct tcttcactga
    19741 tcaattcttc aaaaggggtc attttagctt ttaaagctaa gaaacctgta gcttgcctga
    19801 taccaacaga ggtaagagag aaagacccag aacctttatc ttccttatca ttgaccgtaa
    19861 ttagttcttt cagatcgatc gtggctcccg gtgattctac ataatacggc aagggaacta
    19921 ctacaaatcc aatgatcaac attccaaata tgataatcag tactgttttc aaccattgtt
    19981 tagctttcat ttgattcacg ctttccagct aaagcttcgt tgatcactgg cggtaagtag
    20041 ctgctcacat ctccgccaaa gtgcagtact tcttttaaaa tactggaact cacgtgacta
    20101 tattctggtt ttgctaataa gaagacagtt tccaattcat catatagatg atgattcatc
    20161 tgagcaatct ctcgttcata ttcaaagtct ttgatacttc gtatgcctct gatcaacgca
    20221 tctgcaccaa tctcttttgc tgtttctacc gtcaattgat tttcttgatg catgactttt
    20281 acattaggaa gatgagaaac agcttttgtg atcaatgtca ttctttcttc tgggggaaac
    20341 aaacttttct ttgaagtatt gataaaaaca ccaattacta cctcatcgaa taatttagcc
    20401 gcacgttcca ccgtatctaa gtgacctttt gtaaaaggat caaaacttcc tgggaataat
    20461 gcacgtttca ttgatttccc tcttttctat agatagttac ggccgttatg ccataaatct
    20521 gttttcttat ttgatggaat ccagccacag tttctggcaa ttcgactgtt ttatccgttt
    20581 cgcatacgat aactgcggtt ttagaaaaaa gtcccagttc ttccatttta tggatttgat
    20641 cgattatttt ttgttttgca tatggcggat caagaaacaa gtagtcgaat tgttgtttct
    20701 ccgctgctag ccgttcgatt gctttgtccg catcaagttt tattgtggta aatttctctg
    20761 gttctttcgt aatttcgatg ttttctttga tgatttttag cgcttggtaa tttttgtcta
    20821 cgcataccgc atgactgcag cctctagata ctgcttcgat agctaaacca ccgcttcctg
    20881 caaacagatc caatactgtc tctccatcaa aatatggtcc aatcatattg aatatggatt
    20941 cttttacttt atctgtagtt ggtctggtgt tcgcccctgc taaacttttt aaacggcgcc
    21001 cgccataatt tcctgatatt atccgcatct actcactcct tagaaatggt actggtacta
    21061 gtataccaaa aaaatacttg gacaatgatc aaaaatcgat cgttatccaa gttatttttt
    21121 atttaaaaac tgctcataag agatttttaa agatgttcag ctaatcggat ttctgtattt
    21181 agctcttcaa tttcgaaacc ttcgtctttt aatactgttt tgtttcctat tttttcggca
    21241 aaattcattt cgatatccgg tcggtaagaa cgttctacgt ttcgaacaaa atgtaattta
    21301 cggattcgtt cttctgtttc ttccaatgtc tcttcattta agtaaagcac gacatatttc
    21361 attcttcttg agacataatg caacaagccg aactttttca gattctttaa ttgttttaag
    21421 ctgtagaccc atacgatcag cccgcgtcgt tttgttattt caaattcact attgtctaat
    21481 tgcatgacaa ttacctccgc atccaaagtg ttttttcgtt tcaaaaaacg ggctacctgc
    21541 atctactttg atttcattag aaacttcttc ggcaagtact tggacaattt gatccaacag
    21601 actttgcagc tgtgtttcac tatatcggaa agctgcaaca tgctcatcca tatcaagctg
    21661 tctttttgct tttcgaacag ctcgtttttt ttcacgataa tcaggtgcat actcaccata
    21721 tgccgctaca ttttcaaatg cttctttctt agacaagaac gcttctttca actggcaaac
    21781 ttttgcagat tcttccattg actgtttatg ttcaagataa gaagccatcg ttttactctt
    21841 ttttatcatt tcagcgactt ggaggcattg atcctccagt tgaaacagct cttcgttaac
    21901 gatcactgct atcactactt tctccgtttt cttcttgcaa taagactaac atattttctt
    21961 tagaaaaagc aatgcctaca gaatccgttc ccgggtccat gaaacggtca tggttgtaag
    22021 gatcgctgta ccaatataaa aacgaaaaag tagcgtcaat cgccggatga ataaatagcc
    22081 cactagtatt ttctaatttt tgtttactga ttaacttctc caattcatca ttcgtcagtg
    22141 agaatttcac tgtcgtttga gcactgccta gcgatttttg ccaatcagcc atacgttcct
    22201 ctgacaatat ttcctcaggc cttgtcaaaa aatcatgtag tagcagctga ctgtggtttt
    22261 gaaaggcaag tgtttgattg tatatgggaa gttcgtcttc tcttctaagc atatttaata
    22321 aagtcgtact ttttcgttct ttaacgcgat tgattgattc ccaatcagcc gatttatctg
    22381 cttgataatg tggaaggcct tcaccaaaca cttgatatgg caatagtttc aaaagactat
    22441 ctttatctaa atacacaact gaaaataggc cgtttgttgt ttgatcaaaa aataaaatag
    22501 caaaagtgtc gttatcaaaa gcaattagcg gacgataatt catatcctct tccattagtt
    22561 cgattccaac ttctgtatca tggtactcaa aagtgaaatt agtatagata gtagtgattt
    22621 tcgtaagatc ttgcatcgtc atccctaaat aaaacggcgc gatatcagca ggccctgttt
    22681 gcagtacttt aacagttgtg actctgctgt cttggatgtt cgcttcaaat gtgagatgac
    22741 tagctttatt ttcataatat cttgtttcaa aaccaaatcc gctagttaat cgatcatagg
    22801 cttggccaaa atccttttcc agctgatcga tcggttttcc gatataagta gaaaaaccag
    22861 ttgctttaag ctcctggtat ttccacgttg tcatttggtt tgatatttgt tgatcagagt
    22921 tttccttcac ttcaagtttt ggcggaaaca agactggttc catgtagaat gccacaataa
    22981 ctaatagcag aatcagccaa aacaaaccca gtcttttcat gtttccccac ctttcgctta
    23041 ttatttctca gtcacttcaa tcaaaaggtc accagacgaa atagtgtctc ctgaagacac
    23101 atatacatgt gcgacttctc cgtcaaaacg tgcttcgatg gccgtttcca ttttcatcgc
    23161 ttcagtaatc atcaacgtat cacctttttt aacgttatcg ccttttttca ctaatacatc
    23221 caaaacagaa ccagacatcg ttgctccaat ttgttctcgg tttgtcggtt cagctttcaa
    23281 tcgggtttgg accgtactga tgatcgaatt atctttgata gtgatttcac gtctttgtcc
    23341 attcaaattg aagaacagta cgcgattccc ttcgatatcg ggttctccga tttcatctaa
    23401 acgaatgata agcgtttttc cgcgttcgat ttgtacattg attgtttcac ccaaacgtat
    23461 accttggaaa aatgttggtg tgtccagtaa tgtaacatcg ccaaattgat tatatgcttt
    23521 ttgatagtcc aaaaagactt gcggatacat caaatagctt aatacttcgt cttgttttgg
    23581 ttcatagcca attttttctg ccaattcttt ttttacttcg ttgaagtcga ctgattcagc
    23641 caaaagacct ggacgttcat tcaatgcagg tctgccttta agaataatct tttgcaattt
    23701 tttaggaaat ccgccaacgg gttgccctag ttctccttgg aagaaagtca caacagattc
    23761 agggaaactt agtgtttcgc ctttttcata aatatcttct tctgtaagat cattttggac
    23821 cataaatagt gccatatctc caactacttt agatgatgga gttactttaa cgatatcgcc
    23881 aaacatcaag ttgacagtat gatacatttg tttgatttca tcccagcgat gacctaatcc
    23941 gacagctttt gcctgttgct gtagattcga atactgtcct cctggcattt catgcatata
    24001 tacttctgtt tcaggagcat tcaagccgtt ttcaaacggt ttgtaataca tgcgcacatc
    24061 ttcccaataa tggtttaatt tctgtgcatt ttcaatcgtg atttctggca gccgtggacc
    24121 attgaccagt gcataataca agctgctcat gctaggctga ctagtattcc cactcattgc
    24181 actcattgcc acgtcaacga tgtctactcc tgcttttgaa gcagcagaat aagtgatgat
    24241 tccattcccg ctggtatcat gagtatgcag atggattggc agatctgtcg tttctttcaa
    24301 ttcactaatc aaacgataag ctgcctgcgg tttaagcagc ccagccatat ctttgattgc
    24361 aatgatatgc gcacccagtt gttctagttc ctttgccatg tctttataat actgaacatt
    24421 gtattttgcc cgacttggat cgttgatatc gccagtatag caaatagctg cctctgcgat
    24481 tttaccagta tcccgtacag cttggatact tttttccatt tgtggagtcc agttcaaact
    24541 atcaaagatt cgaaatacat cgattccttg cgctgctgct tctttgacaa attccaccaa
    24601 tacattgtca ggataattag aatacccaac ggcatttgaa cctctgaaaa gcatttgcag
    24661 caacgtatta ggcattaaag aacgaatttt tctcaaccgt tgccaaggat cttcattcaa
    24721 aaagcggtaa gccacatcaa atgttgctcc cccccacatc tcacttgaaa agagttcagg
    24781 aagaccttct ccagtcaaac gtgcaatctg cttgaaatcc tttgtccgaa cacgagttgc
    24841 tagcagactt tgatgggcgt ctctaaatgt cgtatccgtc aacaacaaat tttcctgtcc
    24901 tttgatccat tcgaccaaag catcggcacc cttcgcatct aatacattct tagccgttac
    24961 gtaatcagtt cttgtgatta gatctttagg catacgtggt tcttcataaa atggtttttc
    25021 gccactttcg atacctggaa aaccattcac agtaatttca ccgatatatt tcatcgtttt
    25081 gtttccgcgg tctcgtagtc gaggaaattc aaataatgtt gctgtactat caatgaaggt
    25141 tgtttttgca tttcctgatt ggaattcagg atgtgtgatg acattgagca taaatggaat
    25201 gtttgttttc actccacgga ttctaaattc tttcaagcaa cgttccattt tctgaatggc
    25261 tgtttcaaag gtagcaccat gcgtacatac ttttactagc aatgaatcaa aatacggtgt
    25321 cactacatag cccgcgtaag catttccgac atccagacgt acgccaaaac caccaggtga
    25381 acgatacgta tcgattttcc ctgtgtctgg taaaaagtta ttcaacgggt cttctgtagt
    25441 gattcggcat tgaatggctg aaccatttaa tttaattcct tcttgctgag gcaatccgat
    25501 ttctttatgc agatccaaac cttgagcaat caacagttgc gttgttacga tatcaatatc
    25561 tgtaatcatt tctgtgatcg tatgttccac ttggacgcga gggttgactt cgataaagta
    25621 aaaatcatct ccttcgacaa gaaattctac tgtacctgcg ttgacatacc cgacatattt
    25681 catcaattgg acagctgcct ggcagatttt ttgacgttgc tgttcattca tagagacaca
    25741 aggcgctact tctactactt tttggtgacg gcgctgaaca gaacaatctc tttcaaaaag
    25801 atggatgacg tttccatgcg tatccccgag aatctgtact tcgatatgct taggattagc
    25861 gatgtatttt tcaacataca cttcatcact accaaaggct gcttttgctt cgcttttcgc
    25921 acgttcgtag ccctctcttg cactcttttc atcgtgagct acacgcatgc cgcgtccgcc
    25981 gccgcctaga gctgccttga tcataatcgg ataaccgtat tgtttcgcaa actccaaggc
    26041 atcgtctata gaagcgattg gtccatctgt gcctggaatc gaagcaatcc ccgcttcgat
    26101 agctgcagct tttgctttga ttttatctcc aaaaatatct aaatgatgca gtttgggtcc
    26161 aacaaaaatg atgccttctt cttcgcaccg ttgcgcaaaa ttcaagtttt ccgacaaaag
    26221 accgtaaccc ggatgaatag cgtcagctcc gcattctttc gcgatagaaa tgatcccttc
    26281 aatatctaga tacgcatcaa tcggtttttt tccttgtcct actaaatatg cttcatccgc
    26341 tttgaaacgg tgtacggaat attcatcttc ttctgcatag attccaacag ttttaattcc
    26401 taattctgta caagcacgaa atacacgtac agcgatttca cccctgttag caactaaaac
    26461 ttttttcatc gaaccactcc tacatctttt tttattataa acctaagctc ttgcgttttt
    26521 catccgcact gatgttgagg acaaagccga cacaaatcga aagcatcagc aaactggagc
    26581 ctccttgact taaaaatggg aacgtgatcc ctgtcaaagg aataacgcca gtaattcctc
    26641 caagattgac aaatacttgg atcaaaaaca ttgcgccgat cccaatgcac aaaagcgaat
    26701 tgaacggatc ttttgaacgg atacctacaa ggacaatgcg cacaatcata aagaatagca
    26761 acgctaatat caaaatcccc ataatcactc ccagttcttc cactacgatt gcataaataa
    26821 aatcggtttg tgcttcttgt aaaaatcctt ttttctggat actgttacct aatcccctgc
    26881 caaatagtcc tccgttgaac atggcatagt agccgttgac tagttgatgc cctttattta
    26941 attcgtctga gaaaggattt tggtaaatag caaatcgatt atagatatac tgtaaacgtc
    27001 cgggtaagat ctttccgttt gtgatattga tcagccagat cgtaaaagta cttagacaaa
    27061 agcctcctac tcctacaatc aaagtataca cataattgac tccgctggct aaaagcagaa
    27121 ccaaaataat caaaaagata actgccgcat taccaaaatc gggaagaata gcgactaatg
    27181 cagtaagtcc gataaccatt gcaagtggac gtttcacagt cccaagaaaa tctttttgta
    27241 cactgttttg tcttttagat aaagtcagag acaggtacca gatagaaatg atttttaggt
    27301 attcggcagg ttggatcgag aatcctgcaa tttgcagcca acctttcgcc ccattgatct
    27361 ccttaccaaa aaacacaaca atcagcagca aaatcgtcaa aactgcgatg gctgccatga
    27421 tcaaacgctg gtttttcaaa acatctgttt tcattttata aagtagtgcg atagcaatca
    27481 agctaagcac ccaaaaaata ctctggttaa tgactgatgc ggacggattt tgtccgtttt
    27541 ctaataatag atacgacgta gaactatata ccatgatcaa tcccaataca cataaaatta
    27601 aatagggaat caagatgcta tagtctaata gatgcctttt tttcactttc ttaggcaagt
    27661 ctattcctcc ttgccaactt cttttttatc ataaatttct tggtaaacct cactatataa
    27721 ctgattcaat tctcgttcta gctcacttaa catcagctgg ccctcgttct ttccgattac
    27781 tcctatacgg acagcaaaac cgacttgccg ggaaaagccg tacatctgtg tatctaccac
    27841 ttcttcgaag gctttgcatt gtgagataca gagactgttg ttttgatttc ggattaatcg
    27901 tttgatccgt tctgcttcat catttaatac ttggatcgca agttttttcg acaaagaatc
    27961 catctgtgct tacctcccat tctaattcct taattataac atatttgatt ttcataagga
    28021 gcaaaaaaaa cagatttaac cagttgtttt tattcatttt tacgtattcg ttctttctac
    28081 ttatccagaa cgttagagtc cgcatttttt atccgagata aaaaggcttc gccatattta
    28141 tcaagcttgc tttggccaac tccttttatc tgaagcatct ccaatctgct tgatggacgt
    28201 attcggctca tttctttcaa tgtggaatct gaaaatacga catacggggg aacgcctgct
    28261 tctgatgcta gatccgttcg cagttcccgc agcacttcaa atagtgtatc ggtttcttca
    28321 tccgatagct gctggacttt cttgggttcc tttcgataaa cagattcttg tcctttcaat
    28381 acttgtatcc ctctttctgt tactttcaaa acaggatact caccattgat ggtcagaaga
    28441 tagccattcg atatcaaata ttcaattaac tgcatcgttt ctttttgcga ttggtttttc
    28501 atcagtccat aggtagataa atgtccaaaa cctaaagctt gcagcttttg ttctttcgat
    28561 cctgctaaga ctttcatcag catttgttta ccatagttct cacccatacg ttttaagcaa
    28621 gaaagaacca tctgtgcttc tgtcgttact tctactattt ctctgtcatc taaacagtta
    28681 ccacaacggt tgcacggact gcttgtctcg ccaaaatagt ttaggatgta ttgctgaaga
    28741 caggactcaa tatacacgta ttctgtcatc gcttttaatt tttcatattc tttttgcttt
    28801 tgcccttctt ctctttggga ctgctggata aaaaactttt ggacttggat atcttgagga
    28861 gagaaaagca ggatggcttc actggaaaga ccatctcttc ccgcccgacc agcttcctga
    28921 tagtatgctt ctagagagcc aggaatttga taatggataa caaaacggac attgctttta
    28981 tttataccca taccaaatgc atttgtcgct acaatcagct gcaagcgatc gtataaaaag
    29041 gcttcttgca tttctgttcg ctcattctca tttagaccac catgatatct acccacggaa
    29101 aaatcgaatt ttttaagcaa atgatagaga cggtctactt cttttctcgt tgctgcataa
    29161 atgataccag attgattttt gttgatcttt aaatattcga ttagatattg ttcttttttt
    29221 tgatctttaa ttacttgaaa tcgtagattt tctcgttcaa aaccagtctg aatatggttg
    29281 ttttctggaa tttttaaaag tctttttatg tcagcagcta cttgcacagt cgctgtagct
    29341 gtcaaagcaa caattacagg acgatttggc agttgatcta gctggttcgc catctgtaaa
    29401 taacttgggc ggaaatcatg cccccactga gagatacaat gtgcctcgtc aatagcgatc
    29461 atacggacgt tccatcgctt cattgcttgg ataaaatccg gcatgataaa acgttcagga
    29521 gcgatataaa gtaaacggta ctgctgtcta tcgatttctt ggaagcgtct agctgtttcg
    29581 taaccatcta acgagctatt gataaaagtg gcgggtatac ctaattgatt cgccgcgtcc
    29641 acctgatcct tcatcaaaga gattaaaggg gacacaacca cagtaagccc atctaatagt
    29701 aaagctggaa gctggtaaca gattgatttc ccacttccgg taggcatgat tcctaataca
    29761 ttttcctgat caatgacttt ctggataatt tctttttgac caggtctaaa ctcatcatag
    29821 ccaaagtatt gttttaataa gtcgttcatc ttcccgctcc tttctcttgc cattttactt
    29881 cgattttctt cgaaggacaa gttgatctcc tttcgtgaaa cattatctta ttatgaaaca
    29941 aaaactctct agaaataagt tttttaaaag tcttttaaac gtctttcggc gtattttctg
    30001 aatttatgtc cattgacaaa gatgacttct ccacagaaaa gagttggaca aaaaaagaaa
    30061 ccagctattt tttat
//
LOCUS       DASOUD010000027        29108 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.027, whole genome shotgun sequence.
ACCESSION   DASOUD010000027 DASOUD010000000
VERSION     DASOUD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.027"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1028)
                     /locus_tag="U4A29_001082"
     CDS             complement(<1..1028)
                     /locus_tag="U4A29_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO0680218.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1110..1799)
                     /locus_tag="U4A29_001083"
     CDS             complement(1110..1799)
                     /locus_tag="U4A29_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO0680219.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1905..2273)
                     /gene="rplL"
                     /locus_tag="U4A29_001084"
     CDS             complement(1905..2273)
                     /gene="rplL"
                     /locus_tag="U4A29_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO0680220.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2326..2829)
                     /locus_tag="U4A29_001085"
     CDS             complement(2326..2829)
                     /locus_tag="U4A29_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO0680221.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3033..3160)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3239..3928)
                     /gene="rplA"
                     /locus_tag="U4A29_001086"
     CDS             complement(3239..3928)
                     /gene="rplA"
                     /locus_tag="U4A29_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO0680222.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4032..4454)
                     /gene="rplK"
                     /locus_tag="U4A29_001087"
     CDS             complement(4032..4454)
                     /gene="rplK"
                     /locus_tag="U4A29_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO0680223.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4797..5465
                     /gene="sdaAB"
                     /locus_tag="U4A29_001088"
     CDS             4797..5465
                     /gene="sdaAB"
                     /locus_tag="U4A29_001088"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO0680224.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5478..6350
                     /gene="sdaAA"
                     /locus_tag="U4A29_001089"
     CDS             5478..6350
                     /gene="sdaAA"
                     /locus_tag="U4A29_001089"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO0680225.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6441..7460)
                     /locus_tag="U4A29_001090"
     CDS             complement(6441..7460)
                     /locus_tag="U4A29_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEO0680226.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7633..8538)
                     /locus_tag="U4A29_001091"
     CDS             complement(7633..8538)
                     /locus_tag="U4A29_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO0680227.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="U4A29_001092"
     CDS             complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="U4A29_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO0680228.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9243..9413)
                     /gene="secE"
                     /locus_tag="U4A29_001093"
     CDS             complement(9243..9413)
                     /gene="secE"
                     /locus_tag="U4A29_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO0680229.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="U4A29_001094"
     CDS             complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="U4A29_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO0680230.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9751..10395
                     /locus_tag="U4A29_001095"
     CDS             9751..10395
                     /locus_tag="U4A29_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO0680231.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10451..10954)
                     /locus_tag="U4A29_001096"
     CDS             complement(10451..10954)
                     /locus_tag="U4A29_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO0680232.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10967..11013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11551..12516
                     /locus_tag="U4A29_001097"
     CDS             11551..12516
                     /locus_tag="U4A29_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO0680233.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12615..13787)
                     /locus_tag="U4A29_001098"
     CDS             complement(12615..13787)
                     /locus_tag="U4A29_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680234.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13774..14919)
                     /locus_tag="U4A29_001099"
     CDS             complement(13774..14919)
                     /locus_tag="U4A29_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680235.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14933..15865)
                     /locus_tag="U4A29_001100"
     CDS             complement(14933..15865)
                     /locus_tag="U4A29_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680236.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15982..17061
                     /locus_tag="U4A29_001101"
     CDS             15982..17061
                     /locus_tag="U4A29_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO0680237.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17058..17687
                     /locus_tag="U4A29_001102"
     CDS             17058..17687
                     /locus_tag="U4A29_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0680238.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEFADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17797..18024)
                     /locus_tag="U4A29_001103"
     CDS             complement(17797..18024)
                     /locus_tag="U4A29_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO0680239.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18127..20715)
                     /locus_tag="U4A29_001104"
     CDS             complement(18127..20715)
                     /locus_tag="U4A29_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO0680240.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20735..21394)
                     /locus_tag="U4A29_001105"
     CDS             complement(20735..21394)
                     /locus_tag="U4A29_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO0680241.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="U4A29_001106"
     CDS             complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="U4A29_001106"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEO0680242.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22167..23033)
                     /locus_tag="U4A29_001107"
     CDS             complement(22167..23033)
                     /locus_tag="U4A29_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO0680243.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23142..23783
                     /locus_tag="U4A29_001108"
     CDS             23142..23783
                     /locus_tag="U4A29_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO0680244.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="U4A29_001109"
     CDS             complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="U4A29_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEO0680245.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25224..26123)
                     /locus_tag="U4A29_001110"
     CDS             complement(25224..26123)
                     /locus_tag="U4A29_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO0680246.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26125..26922)
                     /locus_tag="U4A29_001111"
     CDS             complement(26125..26922)
                     /locus_tag="U4A29_001111"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO0680247.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26900..27604)
                     /locus_tag="U4A29_001112"
     CDS             complement(26900..27604)
                     /locus_tag="U4A29_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO0680248.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27735..28310
                     /locus_tag="U4A29_001113"
     CDS             27735..28310
                     /locus_tag="U4A29_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO0680249.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28428..29105
                     /locus_tag="U4A29_001114"
     CDS             28428..29105
                     /locus_tag="U4A29_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEO0680250.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6169 c         5232 g         9143 t
ORIGIN      
        1 acagtattat ttcggttgct tgctgcttca cctagccctt gtactcgtgt ttgattggcg
       61 acttgaccaa attgattccc gacgtctaat aaaataatag ctaaaagcaa aatggccaaa
      121 tggcttccgc caaccaataa agcaataaag cttattgttt gcataacaag tcccatatac
      181 acgatttttc tttcagaata agaatctgcc agtcgtccca cgataggtgc agcaactgct
      241 ccagacaaac caaaaatcgc taatatccct gcttcgaatg ttccccaatt gtaggcggga
      301 ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat
      361 ccactgactg ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg caaactttta
      421 agtgaatcaa ggtatgaaac agctgtacta ctttgctgga cagaacgcgg cattttccag
      481 taaagcagaa gtgtaagaat tccgactgca cataaagcaa ataaatagat catccgccaa
      541 gaaaaaagac tggcaacaat accagatacc gtccgagaaa gcaaaatccc cacgagtaaa
      601 ccgctaagca atgtacccat gaccttccct cttgcttttg gtcccgctag taccgcaccg
      661 taaggaatga tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca
      721 aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag agacggcaga taacgctacc
      781 attcgaatga tcaattttgg acgatccacc acgtccccta aaggaactaa aaacagtaac
      841 cctaacgcat agcctaattg ggtcaacatc gtcaatatcc cgactgcact tgtgctaaca
      901 gataaactag ctgctacttt tgttccaatc ggctggatgt aatacatatt ggcaacaaca
      961 acgccacaag tgattgataa tagtaaaata agcgaagttg ttaaaggtgt tttttcatta
     1021 ctcttcattg atttccccca tttcctatct tagtttacaa cttatggaaa agattggaaa
     1081 tagaaaaata caactattcg ccacacttat tatttgtgct tttgttttcc ttgttttttc
     1141 ttgcgatctt tataaatgaa gacaactaac cctactagca cagtaacact tgcaaccaac
     1201 gtgactgcag ttttgttata tccgtgaaac agagcttctc caccacacgc atacaacaca
     1261 gacgaaggga tgactccaat cacgatcgac aatatgattt gctgtagagg caatttcatc
     1321 gcatctgcag caaaattgac catagaagat ggaataaccg gtatcatata gcctaacatt
     1381 acacctattt ttggatgttt catttgtcgg attgcttgca cccaatgatt cgtttcggtt
     1441 ttcttatcta aaaattttaa gcgatgcata aggaaaattg cagtgatgtt accaagagca
     1501 ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa agctcaaacc agcaatgact
     1561 ccaactactg aagtaggaat acctggaaca gcacacatca ccgctgtcaa tacgatcaag
     1621 agtatcgatg caaaagtccc atgcgaacga atatgtgcga gtagctctgc cttactcgct
     1681 ctcggattta aaaataattg gatgtctgtc tgatattcta aatacaaccg atagcccact
     1741 accagtatca gaatgattcc caacgagatc aacatcaatc ggattatttt ctttttcata
     1801 tttcctcctg aaaaaaatct atattcatta aataatagtt taacataaaa acaaaaaagc
     1861 accgaaatcc aacataggat ctcagtgctt ttaaatagta aaaattattt aacagttaca
     1921 gatgcgccaa cttcttcaag tttagctttc aattcttcag cttcttcttt agatacgcct
     1981 tctttaactg gtgcaggagc gccatcaact acagctttag cttctttcaa gcctaaacca
     2041 gttgcttcac gaactgcttt gataacttta actttttggt ctccagctgc agttaattct
     2101 acagtgaatt ctgtttgttc ttcagcagca gcagcgccac cagcagcagc tacagctaca
     2161 ggagcagcag cagatacgcc aaattcttct tcaatagctt taactaggtc gttaagttct
     2221 aggatagttg cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc cattataatt
     2281 tcctccgttt aattgttctt ttttatttaa tgctttatgc ggcaattaag ctgcatctcc
     2341 atcttctttg tctgccactg ctttgacagc gtaagccacg ttgcggactg gcgcttgtag
     2401 tacagatagc aacatagata gaagtccttc gcgatttggc aattttgcca atgctgtgat
     2461 ttcttccaat gaagagactt taccttcgat gataccgcct ttgatttcta atgcttttgc
     2521 ttctttagca aattcgtcca tgattttcgc tggtgcgact acatcttcgt tactgaatgc
     2581 aacagctgta ggtccagtga atacttcgtc catgccttca aggccagctt ttttagctgc
     2641 acgtgaaaga atagaatttt tgataacttt catttctacg ttagcatcac gaagttgttt
     2701 acgtaaacgt gttacttcat caactgttaa tccgcggtag tcaacgacta ctactgatgc
     2761 agctgctgta aatttttcgc ttacttcatc aacgattgct gctttttttg cgataattgc
     2821 ttcactcatt tttgagtttc acctcctgaa ttttgatgga agagtttttt gcactgaaaa
     2881 ggcaagtcac ttttatagtt actttgctca accagtatta ttcatcacct attcacttcg
     2941 ttcatagtga ttctactcgc tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt
     3001 attgttcatc atctcttcac ttcgttcata gtgattcaca ataaaaaact ctatgccacc
     3061 gcagacatag agggactatt gaatatattc aataaacgtc ctcggtagga aattaaggct
     3121 tgatgccacc tactgtcttc ggtacaagtt aattattaac ctcaactagc ttagcatgct
     3181 aaactagttg agtcaagaat tatttttcag ttggatcaat caacattcta atatttaatt
     3241 agaaagaagc ttggtcaaca tggataccag gtccaaatgt tgttgtgatc gttacatttt
     3301 tcatgtattg acctttagct gctgatggtt tagcttttag caatacatca tggattgctg
     3361 caaagttttc aactaatttt tcatcgtcaa atgaaacttt tccgattggt acatggatat
     3421 ttccagcttt gtccacacgg taagttactt taccagcttt cacttcgttt actgcttttg
     3481 ttacgtccat tgtaactgta ccagttttag ggtttggcat taagcctttt ggtcctaata
     3541 ctcgtcctag acgacctaca gtagccatca tatcaggtgt tgcaacaact acatcaaagt
     3601 caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag
     3661 ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac
     3721 cagttccgtt tggtaataca acagcaccac ggatttgttg gtctgctttt ttagggtcta
     3781 cgttcaaacg gtatgcaact tcaactgttg catcaaattt tgcaatgttt gtttcttttg
     3841 ctaaagctac tgcttcggca acaggataaa ctttacttga atcaactttt tttaatgcat
     3901 cttgcatttt tttgctcttt ttagccattt tggttcctcc ttgattgtgg ttataacggt
     3961 tgtacctccc acgtgttcta tatctttcaa tatagagcca actgagaagc tgctgtctta
     4021 gggtgtatag attattctac agtgatcccc atgcttcgtg cagttccttc aaccatgcgc
     4081 attgctgctt caacgttagc tgcgtttagg tcttccattt ttaattcagc gatttctctt
     4141 acttgatcgc tagaaacttt cgcaacttta tttttgtttg gttcgcctga acctttttcg
     4201 attttagctg cttttttcaa tagaacagca gctggcggtg tttttgtaac gaatgtgaat
     4261 gaacggtctt cgtatacaga aataacaacc ggaatgatca aacctgcttg atcagctgta
     4321 cgagcgttga attcttttgt gaatcccatg atattgatac ccgcttgacc tagtgcagga
     4381 cctactggtg gagctggagt tgctttacct gcaggaattt gcaatttaac gattttttct
     4441 actttctttg ccacgagaca tacctcctta agtccgtgat gtggttaatt ggagatgcat
     4501 atttctcctc ccacgtttta tctgatctga aaaaatccag acaaataatg tgcaaaacaa
     4561 tgcacactgg tacagtatag caaatttatt tgttttttca agattttttt ctgctatttt
     4621 tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt ttcgttctca
     4681 tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc aaacaataac
     4741 acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg gagaaaatga
     4801 aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt ggtccaagta
     4861 gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata tttggcgaac
     4921 agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat cgggggcacg
     4981 gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat gaacgtttag
     5041 ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt cctaagagtg
     5101 aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt
     5161 cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg aatggattca
     5221 aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat gttccaggta
     5281 tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc actatgacag
     5341 tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat gatcctcaag
     5401 tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta aattatttcg
     5461 attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa gcaaaagctt
     5521 atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc cgttcccgta
     5581 gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg
     5641 gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg
     5701 attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct gtacgcaatg
     5761 ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg
     5821 gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt gatttgacac
     5881 atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca
     5941 acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct gcaagtgcaa
     6001 tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag
     6061 tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca ggcttagttg
     6121 aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc tctgctgata
     6181 tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct gcaatgtacc
     6241 aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc ttagctgtta
     6301 cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga taaagctcct
     6361 taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag aggactaatt
     6421 gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc atcgatcgtt
     6481 tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc aaaaacagat
     6541 gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc aatcgcaact
     6601 aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac accagagtcg
     6661 aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac ttcaaaggac
     6721 gcaggacctc catctaattg acgtccacca tgattggata cccagatacc atctgcccct
     6781 gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat aactggaaga
     6841 tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt tgcagctgca
     6901 taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc gctatacgca
     6961 gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc ttcacggtaa
     7021 ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc tactgctttg
     7081 ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa aaactgcggt
     7141 gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt gctgatggaa
     7201 aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt ttcatgtgcc
     7261 aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat tccgaaaaca
     7321 gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat tttctttgta
     7381 ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc gcgaatatag
     7441 ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc aatgtttaca
     7501 atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc ttctttcatc
     7561 ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat ttactctcag
     7621 atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta cttcttggtt
     7681 ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct ctatttcatt
     7741 tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta ctgttccatc
     7801 actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg ttataggaat
     7861 attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag gcccctttgc
     7921 taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat
     7981 tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg
     8041 tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta tgccgtagtt
     8101 ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact cgttattttt
     8161 attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca aatctcctgt
     8221 tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg cttgctgatt
     8281 tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt agccgtggaa
     8341 aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg ttttttcttt
     8401 atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg caaacaaact
     8461 gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg gttctgtcat
     8521 acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca tatgatcgac
     8581 atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt tctgtactgg
     8641 tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg tcaacttctg
     8701 ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact tcgccaactt
     8761 ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata tgattgactt
     8821 cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca
     8881 caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt tcaacaagta
     8941 cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg acttcagttt
     9001 ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata ctttgcgcac
     9061 gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc aagacatacc
     9121 agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata aaaaaacctc
     9181 agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt gtggctagcg
     9241 agttatttta aaatccagcc aaataagctt tggatggctg tatccattac gtaaaacatc
     9301 aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag ttgttttctg
     9361 cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt catgagacaa
     9421 cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta caatatttac
     9481 aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat tttgtgtagt
     9541 tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt tacttctgcc
     9601 cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt tacttgtcaa
     9661 tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa tttgaaatta
     9721 agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt atataagaaa
     9781 aaagatctga caaccgttac agagactagt acattagaag aagcattaaa aattttagaa
     9841 acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt ccgtggaaac
     9901 atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc
     9961 actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt cttcaaagta
    10021 ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa tcatttttat
    10081 ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag cgtagaacaa
    10141 ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc tgctattgca
    10201 aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat cgaaaaagac
    10261 gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa
    10321 acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga aaatttgcaa
    10381 aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt tcttttttcg
    10441 ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg
    10501 gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa
    10561 atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga
    10621 agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg
    10681 tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac
    10741 caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa
    10801 agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag
    10861 gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta
    10921 cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt
    10981 tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac
    11041 ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta
    11101 aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc
    11161 ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat
    11221 aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga
    11281 agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg
    11341 cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg
    11401 ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat
    11461 tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata
    11521 tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat
    11581 ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa
    11641 aataagcata ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt
    11701 gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc
    11761 gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat
    11821 actttctatc agatggaagt agaattccca gttgcttata ttccagcagg ttctggaaat
    11881 gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta
    11941 gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa
    12001 aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca
    12061 aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta
    12121 ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga
    12181 ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt
    12241 ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc
    12301 gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa
    12361 aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca
    12421 acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc
    12481 acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta
    12541 catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt
    12601 tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta
    12661 cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg
    12721 aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta
    12781 ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag
    12841 tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg
    12901 atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg
    12961 atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag
    13021 gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa
    13081 ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg
    13141 tttcgtttac tgatctcttt gcgattaaac gctagattga tgacgctgaa accgctaaaa
    13201 atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc
    13261 atccgctgac tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt
    13321 gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc
    13381 aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt
    13441 atcttgatcg tcgccatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt
    13501 aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca
    13561 tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc
    13621 gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca
    13681 atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc
    13741 gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct
    13801 ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt
    13861 ggtataaaaa ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa
    13921 gttgacgaga tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg
    13981 ttccgacccc atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct
    14041 atccttttgc accagattca tttttggtat caaattacca accaatgtac ctaacaaaat
    14101 cgcacacaac gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc
    14161 tacttgatag ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt
    14221 aaagcttgct gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa
    14281 gcgaatacca tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat
    14341 cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat
    14401 attacgacct gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg
    14461 cgcgagatca acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa
    14521 aaattgatcc atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag
    14581 cgaaacagat gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc
    14641 attcgcttct acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac
    14701 ttgttctaag actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat
    14761 tccagtatta atcgtttcaa gattgccacc ttgatcaatc tcgccaaagg ccgccataaa
    14821 catcaatcct aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag
    14881 cagtattttt gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc
    14941 tcaaatcctt gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta
    15001 tttttccgta actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat
    15061 ttagttcagt agttgttact tggagctgac cttttttata agcgattcca tcagaagcta
    15121 ttgttcgctg aatcgctgac aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac
    15181 gatccgccgt ccgtaccata tcttttagag attctttggt tcctttagca atcacctgtc
    15241 cttgatccat gatcacgatc tggtcgcaaa gaatttctac ttcttccatg taatgtgttg
    15301 tatagacaat ggttgctcct tgtcgattca gctccttgat actttctaaa attttattcc
    15361 gactttgggg gtctacagct actgtcggct catctaaaaa aattaaccgc ggcttgtggg
    15421 caatcccaca tgcgatgttc aaccgacgtt tcaagccacc actaagttct cctggacgaa
    15481 acgaaacata tttttctaat cccactaatt gaatcacctc ttcaatcaag gctttccttt
    15541 tctctttttc ggaaacatat aaaccgcaaa aataatcgat attttcttgc acacttagtt
    15601 cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat tgccttgaca gcataatttt
    15661 caggagtcat tgttgtcccg aacaatttaa tggttccttt gtcataattt aacaaagaaa
    15721 gcatacagtt gatggcagtc gatttcccac tgccgtttgg acctaacaga cctaagactt
    15781 ctccttcttg tacttgcaga tcaaaatgat tcaatgctac aaaatctcca tagcgtttga
    15841 ctaatttatc aatttcgata atcatcatga aaaactcctt ccaaatttct tgttgatctg
    15901 actataataa agtgcaagaa aagaaactag tgacgattgg ctaaaccaca catgacattt
    15961 gtcacaatga aggagtctgc tatgcataaa tctcggctac tcaacgaaac tagctttcta
    16021 ctggttatct cattgctttt gctggtaagt caaggattcg ttgctatgca agttatctat
    16081 ttattgatcg ccgttttatt cactggtgtc agttatttac tgcctacaaa gccaaattgg
    16141 attttccaag ttattctatt tcttttatgt gcctttttac ctgatcttgt cctgtttctg
    16201 ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc ttgattactt gtttttgtta
    16261 ggttttttgt tgatttcgac ggttggtaat catcccccca tcaaggtcgc ctttgtcgta
    16321 attttgctgt ttttttcctt ttacttacga aaaaaagatc aacgaacgac cgatcttgag
    16381 aaaaagtaca ttgtctccaa agatactcat tgggaaaaac aacaagagtt atcggaacaa
    16441 aataaagcat tggccctgtc acaagaaaca caattagcct tgcaaattgt ggaagaacgg
    16501 aaccgaattg cccgagacat ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta
    16561 caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc attagaaaat
    16621 ttaagtgcca ccgttcacac tgggatggat cggattcgtc aaagtgtcca tgacctccat
    16681 gagacatcga tctcttttca acactcctta gagtttctga tcgctgattt tccttcttct
    16741 gttacgattg aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta
    16801 atggtcatca aagaagcctt aaccaacagt atcaaacata gtcatgccaa aaccgttgtt
    16861 ttacattttc gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga tggcaccagc
    16921 ccacaaaaaa attctgctcc cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa
    16981 caaaagggac aagtccatat tttccaagaa aaccagcaat ttcagttaac gatcatttta
    17041 ccaagggagg gacaccaatg ataacagtag tcattattga tgatgatcca tttgtgacga
    17101 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    17161 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
    17221 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
    17281 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
    17341 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
    17401 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
    17461 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
    17521 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaatttgca gatgccttgt
    17581 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
    17641 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
    17701 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
    17761 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
    17821 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
    17881 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
    17941 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
    18001 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
    18061 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
    18121 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
    18181 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
    18241 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
    18301 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
    18361 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
    18421 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
    18481 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
    18541 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
    18601 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
    18661 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
    18721 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
    18781 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
    18841 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
    18901 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
    18961 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
    19021 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
    19081 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
    19141 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
    19201 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
    19261 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
    19321 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
    19381 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
    19441 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
    19501 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
    19561 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
    19621 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
    19681 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
    19741 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
    19801 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
    19861 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
    19921 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
    19981 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
    20041 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
    20101 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
    20161 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
    20221 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
    20281 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
    20341 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
    20401 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
    20461 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
    20521 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
    20581 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
    20641 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
    20701 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
    20761 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
    20821 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
    20881 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
    20941 cgaataaaag tgggccatct ttatgtcggg caaccgcatc tgctacaaca gtttcgatac
    21001 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
    21061 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
    21121 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
    21181 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
    21241 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
    21301 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
    21361 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
    21421 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    21481 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    21541 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    21601 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    21661 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    21721 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    21781 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    21841 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    21901 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    21961 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    22021 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    22081 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    22141 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    22201 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    22261 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    22321 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    22381 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    22441 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    22501 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    22561 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    22621 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    22681 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    22741 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    22801 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    22861 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    22921 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    22981 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    23041 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    23101 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    23161 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    23221 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    23281 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    23341 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    23401 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    23461 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    23521 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    23581 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    23641 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    23701 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    23761 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    23821 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    23881 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    23941 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    24001 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    24061 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    24121 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    24181 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    24241 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    24301 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    24361 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    24421 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    24481 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    24541 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    24601 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    24661 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    24721 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    24781 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    24841 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    24901 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    24961 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    25021 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    25081 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    25141 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    25201 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    25261 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    25321 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    25381 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    25441 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    25501 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    25561 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    25621 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    25681 aatccatgct ttgcaaaaag catcaagcca gatgtatccc gatctagtcg cgtcaccaca
    25741 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    25801 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    25861 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    25921 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    25981 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    26041 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    26101 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    26161 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    26221 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    26281 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    26341 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    26401 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    26461 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    26521 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    26581 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    26641 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    26701 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    26761 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    26821 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    26881 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    26941 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    27001 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    27061 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    27121 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    27181 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    27241 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    27301 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    27361 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    27421 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    27481 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    27541 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    27601 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    27661 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    27721 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    27781 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    27841 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    27901 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    27961 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    28021 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    28081 taatccaaac cggaaagctt acaacaaaac gagctgaatt ccctattgaa gaaggacttt
    28141 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
    28201 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
    28261 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
    28321 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
    28381 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
    28441 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
    28501 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
    28561 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
    28621 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
    28681 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
    28741 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
    28801 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
    28861 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
    28921 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
    28981 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
    29041 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
    29101 attaaaaa
//
LOCUS       DASOUD010000028        27771 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.028, whole genome shotgun sequence.
ACCESSION   DASOUD010000028 DASOUD010000000
VERSION     DASOUD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.028"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..495
                     /locus_tag="U4A29_001115"
     CDS             133..495
                     /locus_tag="U4A29_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEO0680251.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            504..1256
                     /locus_tag="U4A29_001116"
     CDS             504..1256
                     /locus_tag="U4A29_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680252.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            1270..3072
                     /locus_tag="U4A29_001117"
     CDS             1270..3072
                     /locus_tag="U4A29_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680253.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            3349..4389
                     /locus_tag="U4A29_001118"
     CDS             3349..4389
                     /locus_tag="U4A29_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEO0680254.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(4441..6585)
                     /locus_tag="U4A29_001119"
     CDS             complement(4441..6585)
                     /locus_tag="U4A29_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEO0680255.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(6942..8315)
                     /locus_tag="U4A29_001120"
     CDS             complement(6942..8315)
                     /locus_tag="U4A29_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO0680256.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(8315..8710)
                     /gene="rbsD"
                     /locus_tag="U4A29_001121"
     CDS             complement(8315..8710)
                     /gene="rbsD"
                     /locus_tag="U4A29_001121"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO0680257.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(8738..9655)
                     /gene="rbsK"
                     /locus_tag="U4A29_001122"
     CDS             complement(8738..9655)
                     /gene="rbsK"
                     /locus_tag="U4A29_001122"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO0680258.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(9763..10776)
                     /locus_tag="U4A29_001123"
     CDS             complement(9763..10776)
                     /locus_tag="U4A29_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO0680259.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(10857..11456)
                     /locus_tag="U4A29_001124"
     CDS             complement(10857..11456)
                     /locus_tag="U4A29_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO0680260.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            11642..12343
                     /locus_tag="U4A29_001125"
     CDS             11642..12343
                     /locus_tag="U4A29_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680261.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            12353..15064
                     /locus_tag="U4A29_001126"
     CDS             12353..15064
                     /locus_tag="U4A29_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680262.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            15461..17089
                     /gene="treC"
                     /locus_tag="U4A29_001127"
     CDS             15461..17089
                     /gene="treC"
                     /locus_tag="U4A29_001127"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO0680263.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(17149..18330)
                     /locus_tag="U4A29_001128"
     CDS             complement(17149..18330)
                     /locus_tag="U4A29_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO0680264.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            18582..20105
                     /locus_tag="U4A29_001129"
     CDS             18582..20105
                     /locus_tag="U4A29_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO0680265.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(20174..20902)
                     /locus_tag="U4A29_001130"
     CDS             complement(20174..20902)
                     /locus_tag="U4A29_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0680266.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(20915..22195)
                     /locus_tag="U4A29_001131"
     CDS             complement(20915..22195)
                     /locus_tag="U4A29_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO0680267.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(22336..22938)
                     /locus_tag="U4A29_001132"
     CDS             complement(22336..22938)
                     /locus_tag="U4A29_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEO0680268.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            23183..23389
                     /locus_tag="U4A29_001133"
     CDS             23183..23389
                     /locus_tag="U4A29_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680269.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            23701..24420
                     /locus_tag="U4A29_001134"
     CDS             23701..24420
                     /locus_tag="U4A29_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO0680270.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(24501..25184)
                     /locus_tag="U4A29_001135"
     CDS             complement(24501..25184)
                     /locus_tag="U4A29_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO0680271.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            25402..27588
                     /locus_tag="U4A29_001136"
     CDS             25402..27588
                     /locus_tag="U4A29_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEO0680272.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         9227 a         4958 c         5504 g         8082 t
ORIGIN      
        1 ttgtcttgaa ctcttttttt agttgtttta aagaaaatga caaaattgaa agaagaatga
       61 tagttgattc gaaggcagaa ttgttcgctt ttttctataa tcagtatata aagtaagaga
      121 gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg ttttcatcgt tgtggtactt
      181 gcaggggtga aaatcgcaga ttacgtagtt atgggtggag aagaatacta tgtacaaatt
      241 acgacagatg gtacaaaaac agtttcaaaa tcagactctg gacaagaaac agtcaactat
      301 agatatatcc tgcctggcta tacgaaagag ggtaaggaaa aagaactgga cttcaaggga
      361 caattgactc gaccgcttcg taaaggagca tatttaaaaa tcacctggaa caaaaataaa
      421 ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc cgaaaacagc actggaaaaa
      481 ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga agtacagaat gtaacaaaag
      541 tatacggaaa aaacaatgag aaaaagacgc aagcattaag cggcatcagc ttcgatgtag
      601 aaaaaggcga atttattggg atcatgggcg cttctggttc tggtaagtcg acacttttga
      661 atattttatc cacattagat aaaccaacag atgggcatat ccgaatcaat caaaaagatg
      721 tgaccacatt aaaaggaaat caactagcag attttcgtgc gaatgagatt ggctttattt
      781 tccaagattt caatttatta gaaagcttga cagcacaaga aaatatcgct gttcctcttt
      841 ctttacaagg cgtcagacca aaagaaatca agcaacgtgt ccaaaaaatt gcagaacgtt
      901 tatccatctc tcatattttg gaaagctatc cttctgagat ttccggtgga caaaaacaac
      961 gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat tctattaggg gatgagccta
     1021 caggtgcgct ggattccaaa agtgcccggg atcttttaga tacgatggac gaattgaata
     1081 caaaagatca tgtatcgatt cttcttgtga cgcatgatcc gttttctgca agttattgcc
     1141 aacgtatttt attcatcaag gatggcggta tccatcaaga agtgaaacgt ggagatcaaa
     1201 gccgtgattc cttctatcga gaaatactat ctattcttgg aaacttggaa cagtaggggg
     1261 agattgacta tgctttggaa attatcactt actgggatca agagccgttt aagagactac
     1321 ttggtgttat tctcaggatt agttatggca tctgctattt tttacatgtt tgaatcaatg
     1381 gcaagcaacg aagctttttt gaaaagtaat acgatcgttg cggctacagt tatcattttc
     1441 cgattcggtt ctgttttact agggatcatc acattcgttt atattttata tgcaaattcc
     1501 tttttgatga cgatgcggca aaaagactat gcaatgttta tgatgctggg agcaaaaggc
     1561 agaaaaatcg cacagatgat tttttctgaa acattcttag tagggatcgc agctacttta
     1621 gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc accgattatt agtggatcag
     1681 ttagatctca atatttcgca ttttacacct tttagtattc acgggttgtt gatcactgtt
     1741 ttgttttttg ctgttttgtt tttattagct gctgttgtca atgctttttc gattgtcaaa
     1801 aaaccgattt tagcattgat tcgtgcggat cagacaccaa caagaatcaa gcagcataaa
     1861 ttcttgtttt tccttgaagt agtactggga attgcttttc taggctgcgg ttactatatg
     1921 atgaaacacg tggggaccta tcaagtcttt ggtatagggg tagccttagt tacgatcata
     1981 ttaggaactt atttcatttt ccattctgtg atcattttct ttttatcatt actaaagaaa
     2041 tcagaacaga tttcattgaa aaaattgaac aatttcacat tgtcacagtt aaactttcgt
     2101 attcgtgaat atacacaaat gctctcaatg gtcgctatgc tgtttgcctt ggcactgggc
     2161 gctttgacgg taggattagg ttttaaaaat gaaattgcag acttgacaga aaacaattct
     2221 ggatatgatc tagtgatgaa taatcctcgg gcagaagatc agcaaaaaat caaagaactt
     2281 tcaccaacac tttctgcttc ttatactcaa aaagaagatg cgggaacgat ttactataat
     2341 gcaagtgaat ttgcggctca accattgatc atgataaaac atgaagatgg aacaccgccg
     2401 aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg aactgactgt acaagaggaa
     2461 cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa tcaaggtagt cagtcaatca
     2521 gaatttgatc aactgaactt gccacaatcc actttacagc tagttcaggt aaaagacttc
     2581 caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa ataaaatgaa caatccatct
     2641 ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt atgaagtgta taatggtcta
     2701 ttctctggtt ttgaatttat gggattcttc ctaggtttag ctttcttgac catgcttgct
     2761 agctgtttga tgttcaagat cttgtcagga agcaagagtg acattgctcg ttatgaaatg
     2821 cttgaaaaga taggaacacg acgcgggttg cttaaacaat cgatccgtag agaagtaggc
     2881 gtgttattct tagcaccagg aatcttaggt gtagtccatg tcttattcgg attacaaatg
     2941 tttggtctat tcatgcaaaa tccatacaaa gatatttggg ttccgtttac gatcttcttc
     3001 gtattgtatt tcatctacta tgtactgacg acatggttat atactggaat cgtattgaaa
     3061 aagagattgt gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagct
     3121 cctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     3181 gttcggaaaa agaggttgtg acaatatttt tgtcacaacc tttttttcac ggtcttttat
     3241 tagaatatag taaatttttt ctcgttactc acggaataag ctgttaagaa aacggtttcc
     3301 atgtatactg gaatcagtgc aaatgaaaga aaagagggtg caaggaacat ggaaatcaat
     3361 gaacaaaatt ttggcgaaaa ctatcacttg attacgatca cgaatcaaaa tgggacatct
     3421 ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc aaacaataat cgataatgaa
     3481 aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat atctagaaaa agacccttat
     3541 atcggtgctt caatcggacg tatggctgga cggattacga atggcacatt caaattagat
     3601 gatgaaacgt tcgaagtaaa aacagacccg gacactggtc attgtttaca tggtggtgca
     3661 cctagctttg aagccaagca gtggcattat acagtcatca atggtgaaaa cgaagcatcg
     3721 gtcattttct atcagactag cccagatatg gaaaacggat ttcctggaac tttagatgta
     3781 gaagtacggt atacgctgac gaacgatgat acatggcgag tgacggtcca agggatcagt
     3841 gaccgcaaaa cagtattcaa tcctacaaat catgtctatt tcaatttaac cggtgatcca
     3901 gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc gattatttgc tacgctcaat
     3961 aaagatacaa cgcctacagg agaagtagtt tcagtaagtg gcacagcatt tgacttccaa
     4021 cgtcctaaaa aattaggtga ggtctttgcc agtgattttc gccaatcaat gatagtgggt
     4081 ggcatcgatc atccattttt gttgaaaaaa caatctggtt tactaaatgc tgcggaattg
     4141 acaagcccag atgaaaaagt atcgatccaa gtaaaaacag atgcttcagg aatcgtgatt
     4201 tttacagcaa attttggaga agatggaccg gaaatgcgcg ggaataaatt agtaaatcat
     4261 ggcggtatca catttgagac tcaagaactg ccaggagcgg agcgacttga aagttttgga
     4321 gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa cggaatacaa aataaatata
     4381 aagaaataga ataaattaaa aagggagtgt gacagtgtca catccccttt ttaatttatt
     4441 ctatttcttt aaaggcagat caagccagaa tgatttgtct aaacttaaca aggttagtac
     4501 aggaacagtt gcagctttaa ctgtacgagt agttgtttct ggtatctgtc ttgccgtaaa
     4561 attgttccac aaatcccaga ttgaagcgac acggaagatg gaagaagcgt tgttgacgcg
     4621 ttttatcgtt gaaaattcta atcctgtgat tggcacgcca tctgttgtcc atttgattgg
     4681 cacatcgaat cgcttagaca tattgaggat ttcagcgttt gcttcttcag ttgttgctgc
     4741 ttcttttttc ggttcatcat attttgtcag gtcgtacgct tcgatgatcg cgttcaattt
     4801 ttgtgaatat tggcgatctg tagcataacg tccttccaaa tatttggttg cttctttgta
     4861 gtcttttgtt tccgacttcc aagttggttt gtagaaatca ggattgccag tcagtccatt
     4921 tttgatcaag tcggcatagt cttccaaact ttccttgtaa gaaggatatt tacggaattg
     4981 tgcgcggatt tggtagcttt gtccggaact atcttgttca agtgtattga aggtcacgct
     5041 gcttccttta tatgatcctt tgataccaaa aaggttatga tttggttcgc ttgataataa
     5101 gctgtttcca gatccacttt caaggatagc ttgagccatc atgactgatg cgtagattcc
     5161 ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt ttcaagaact gatcagttgt
     5221 ttggttttga ctgaaatgga acgagccatc taatccatct aaaatagatg agccgcctgt
     5281 cagcgaacct gaagcagcag gtaaacctgc taagtttact cggacagcaa aatcaggcgt
     5341 gaaatctgaa atagaagtta ctcgaacttt atctcctggt tgaggcgcat ggataaattg
     5401 tccgttccca atatagatac ctacatggtg agtaacaccg gaagaaggtc cccagaagac
     5461 gagatcgcca ggttgggcct gacttactgg aatctgcgta cctgcatatt gctgttcgcc
     5521 tgtccagcca ccgatataat gaccggttgc tttcatatat acgtaataag tcaaaccaga
     5581 acagtcaaaa gtatttggac cttttgctcc ccatacatat ggttttccaa gatgcttgaa
     5641 tgcttctcga acgattgctt gttggacatt cgttgtattt gtaggcaata cagcatcaat
     5701 tggtgatttt acaacaaaat cagaagaagc agaagatgga ttctctatac cgctttgatt
     5761 tgctgtattt ggacgagatg aagcattgtt cgttccgcca ttcgatgaac cattgtttgg
     5821 atggtttggt tctctctgag tatttccatt gttattcgat ccagatgggt ttgtttgatt
     5881 cgaagaattc ccgctgttcg aattattgtt attattgttt gaattatttg aaccattatt
     5941 attgttgtta ttacccacat ctggtttgtt tggcgtttcc ggatctggtg tactagggac
     6001 ttcttcacta ggatcagttg gtttctctgt tgaagtatcc ggtccagctt taaattctgc
     6061 agtaatagat aaattgttcg tacctactgt aaaggtcttt gcatcataat ttacttcttt
     6121 ggcaccatta acagcgactg aagcaatttg ttcaccttca gctggcgtaa tagtgtaagt
     6181 taccttcgtt tccgctaata aaccttctac cgttccactt tccgtaatat ccaaaggttg
     6241 ctgttcaggg gaatccgctg tcgaagcagt cagttggatc ttaccaatca aagaaggagc
     6301 aattgtaagg gaatatgtct cggctggctt ttcattttct gatggatcag ccggatcaat
     6361 ttcagttggc tcggtcgatc ctgttgttga gttagttgtt ccagttgggt cttctgtaaa
     6421 agaatccgtt gtttcactag tagctgagct ggatgtagtc gtatctgttg tgactgtttg
     6481 atcagctatt tgattatcta cggcaaatac ggtattggtc gacaaaacag acggagcaat
     6541 cagccaagta gcagctagca aacgtatcgt ttttttattt ttcataataa actagtcctt
     6601 ttcctttcat tgattttctg tcttattcag cattcattag acattttttg gaacagataa
     6661 tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg tattctaaat aaaacaattt
     6721 gtaaataagc aacgagcatt cattcgtttt tctttgacgt tcattttatt gcatagacca
     6781 cctttgtttg gggataaaca agtggggata aaaatggtta tgcatatgta aattatacca
     6841 aagtttggaa agtatcacaa aaatttataa aaataatgat cctaaaaaga aaaagactga
     6901 aatatgtgat aaacatctca tacttcagtc tttttcatta actattcata agacattagg
     6961 tcttcatcaa acccgctaac gagagtagtg atcattgcta cgactagact aacagccagt
     7021 cctgctaagt accagaaaaa agtaccggaa atgaatgcag gcaatgtggt cagactacca
     7081 aatacaaaag cgatcgcttt cacttgggca aatcccatta cagctccacc agcagctgca
     7141 ccaatgcaag cagcaacaaa tggttttttc aatgggatcg caattccgta aacagttggt
     7201 tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac cggagatcga acgggttttt
     7261 gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag ccattccaaa attggctgct
     7321 cctgtcaacg gaacgattcg gtcgaaccca taagcagaga tattactgat catgacaggg
     7381 tttacagccc attggatacc aaatattacc aaaatattcc aaccaccagc gatgattgcg
     7441 ccactgatcg gtccgttaat atccatcatc caagtaattg cggcagctaa accttcgcca
     7501 aggtaaacac ccgcaggccc gaataccatt aacgtcaaag gaaccgtgat aactaatgaa
     7561 agtaaaggta caaatactaa ttgcatattt tcgcgaatat attttttgag gaatttttca
     7621 acaaaagaat aaaagaaaat acctaaaatc gccggcatga cagtagacgt atactgcatc
     7681 agtactacag gaatccccag aaaatcagta gctgctttgc cactgtctga aagtaaagag
     7741 gtcagattcg gttcaatcag tgctgcagca atgacagccg caatatatcc attcacttta
     7801 aacgttttgg cagcagaaaa agcaagaaag attggcagaa aataaaagac ggcattcgac
     7861 gcagcagaca aaatatgata agttccgctt gtttcactaa gccagccaat cgtagtcgct
     7921 aaaacaacca gaccttttaa tataccagaa ccggcaaaca gcggaatgat cggcgtaaag
     7981 atttccgata tcaaaccaaa tagtttttcg atcggattta gcttttcttt cggagctgaa
     8041 tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca atccctgatc catgattatt
     8101 tctgcatatc tatcgagtaa aatgaaatac ttaatggaag ggatttgctg ttttaatttt
     8161 tctgcattgg ctactggctg ttttttcaat ttgatacgaa gccgagtcgc acatggaagg
     8221 acagaatcaa tattgtcttt tcctcccaac tgttcaatca gcatatgaat ctgctcggac
     8281 agtaccatct gttcaggatt cattgtcgtt tccattagaa gaccactcca ctcactaata
     8341 taatattggc gtaaggtgtc atttcgcctg tacgaataaa tgcatgacag ttattcaact
     8401 cttttttcat ttcttcatgt gggataaagc taatcggtgt atctggtaac aataatttga
     8461 ttttttccaa tatttccggg ttcattgtct ttatttcttc cgccaattca atcgattgta
     8521 cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa acttggaaga ccgtttgaga
     8581 cagccaaatc tattttttca gtaccaaagg ggacaggcat gcctgcatcc ccgatactta
     8641 atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat atctgaattg attacttttc
     8701 cttttttcat gtttgtttct tcctctcatt gttaatttta gactgctgat tgttctaaag
     8761 cttcgcggta agggatagaa ggttgcgcac cataagactg cacagcaaca gaagaggcta
     8821 gatttccata aaggatcgct tctttcaagt tactaaggtc tggctgaagt gtgctgctta
     8881 atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac ggcatccact ttaaaagcag
     8941 ggacgatacc gtgttctttt tctgtatgat agaaagcgcc ggcactgcct agtgtaatga
     9001 ttactgttcc aatacctaat tcatggagtt tttcggctgc tgcgaccaaa ctgttatgat
     9061 ctgtcactct gattcctgta atgatttctg tttctgtttc atttggtata atgatatctg
     9121 tcttttcaag tagttcagta ggaatcgttt cacgagctgg tgcaggattc aggatcgttg
     9181 tttttcctgc gtcttttgca atagaaaaag ctaggatcgt cgcatctaag ctactttcaa
     9241 actgagcaat aataaagtca ctgtttgtaa tcagttgctt gttttttagg acttcttgtt
     9301 catggaaagc attatttgcc cctgcatgga tcaagatact attttctcct gctgcgtcta
     9361 ccataatcat tgcttgtccg gttgtggcgc cttggatttc agctatccca gatgtatcga
     9421 tgttttcttt tgtcagtaga tctaataact gttgtccttc gctgtcagcc ccaacgcctc
     9481 caataaaaga tgtccgtgcg cctgaacgtt tggctgccac tgcttggttg gcacctttac
     9541 ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc gcctggcttg ggcatgttgg
     9601 ttaaacgaag ggtagtgtcc atattaatac ttccaattac tgtaaccgtg ttcattttac
     9661 aaacctccaa ctatctcatt tattttgatg ggaaaattat tttttaaaac gttttagtaa
     9721 aacgttttaa tagttcgata aaaatatagc acaccttgaa agctatttca agggtgcagt
     9781 tgaaaatctt ttttctaatc gtacaggtaa tcttttttct tcccattctt tttctgggaa
     9841 ttggatccga tctaacaata acttggcaga tgtttgtccc aattcgaaga ttggttgtgc
     9901 gatcgttgtc agtttcggct tgatatactc gcacatatct atattatcat agccaataat
     9961 gctgtagtct tcagggatgc tttttccagc ttcctctaat ccgcgatata atccaaaagc
    10021 taattcatca tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt ctgctgtgat
    10081 ctggtagcca ccttgttttg agaactgtgt aggtaagagg atcagttggt cgtgagggat
    10141 ttggtaaaca tcaagagcag atttaaaccc ctcaatccga gcgtggacat tttccggtgg
    10201 attttcagga tagacaaggg cgatcgtttg atgtcctaga ctaagcaagt gcatgcccgc
    10261 taggtaacca ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt cagatttttt
    10321 ctgatccaaa acaatgaagg gccggccttg ttttttcaaa ttttcgttga ttgcatctgt
    10381 agagacagca gaagtcgcga taatgaatcc atccacacct cgacgtgtga gctcggctag
    10441 atattcgatt tctttttgat gatcagaatc agcattacat aatatcgtga cgaaattctg
    10501 cttatacaag atatcctcaa ttcctcgcat cagtgtactg aagaatggat tcgtgatatc
    10561 aggaacgagt acaccaattg ttttcgtttc cttggtaatc atttgtctag caaaatagtc
    10621 aggctgatag cctaattctt ctttggcagc taaaactttc tgaacagttt tcggactgaa
    10681 tttctccgta tttccattta atatcaagga gacagttgtg attgaaacgc cggaaagttt
    10741 agcgacttct ttgattgtga tctttttttt caccatgttc atactcctat ctaaatcctg
    10801 tggttatttt cccatgaaat caaaaagaag taaatgaaaa tcacgtgtgc cagtacttat
    10861 ttgctttgtt ggatagcttc gattatttgt ttcgttgagc gtactcgtgc agttttcgga
    10921 aatatctttt ttatgaacaa ttgatgggat tctttgtcac gatcactgca agcatcttca
    10981 gcaagcacga tatggtaccc gtgttgaaag gcatctaatg cagtagcata tacaccgttt
    11041 gttgtcgcta caccgcttaa aatgatagtt tcgattccac gccggcgaag ttgaagatca
    11101 agagaagtac caaaaaaagc acttggattg tattttttga tagtcaacat attatctgta
    11161 tctttcaaag aatccttcaa aagtaaatca gtgaattctg gaggtacaga agcagtcatt
    11221 ccacccatat cagtttgttg actcaaataa gggaaagtgg agatgtctac gttcactaaa
    11281 actttcaagg cagcggtacg tttcaatgtt cttactaaca aatcattgtt tgtcagaata
    11341 gatgctgcag tgtgtggaga gagtgtaccg ttatttacga tctctttttg catgtcgatc
    11401 gcgacaaaag cagtttttga taaatcaaaa atcatatttt cctttaaatc attcatgatg
    11461 ttccttcttt caaataacaa tgttttttat gtaagtaaat cataaatcgt ttatttgtca
    11521 actaaattaa aggattcctt tctttagtct agagaaattc agtggtaaaa taaataagtg
    11581 tgagaaaacg cattcgatgc atagatatag cgagaaatat cgtgaaaatg gaggaatcag
    11641 agtgaagtat gtcgaagtaa aaaatgaata caaacgatac aaaatgggag aaacgaccat
    11701 tacagcaaat gacgatattt ctttttcaat tgaaaaaggc gaattagtga ttatacttgg
    11761 tccaagtgga gcaggaaagt caacgattct taatatttta ggtgggatgg atacacctga
    11821 tgaaggtcag atcattattg atgacacaga tattgcgcaa ttttcagata aacagcttac
    11881 tgcttaccga cgaacagatg tagggttcgt tttccaattc tacaatctcg ttcctaatct
    11941 aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct cctgatgcgc ttgatcctgt
    12001 agaggtattg aggcaagtgg gcctagctca tcgattgaat aactttccgt cccaattatc
    12061 aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta gctaaaaatc ccaaacttct
    12121 tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg ggcaagcaag tattgaaatt
    12181 attgcaaaac gctagcagac aacacggcaa taccgttttg atcattacac ataattctgc
    12241 tttagcgccc atcgctgata gagtcattca cattaatgat gctaaagtaa ggtctgttga
    12301 attaaatgat catccttcat ccattgatga gattgtatgg tagggggatt ttatgaaaac
    12361 aagaacatat cttaaagcag gttttcgcga aattcggcaa tctaaaggac gattcattgc
    12421 aatcatcctt attgtcatgc taggaacact tttatttgta ggtgtcaaaa ctgctggacc
    12481 tgtcatgcaa aaaacgatgg atcactatgt tggtacagca ggactttctg atttgcaaat
    12541 cgtttctact ggtggcttga cacaaaaaga tattgctcaa gcagaaaaga tcccagatgc
    12601 aaaagtagaa acaggaaaac agatctatta ttccaatact ggcaaaaacg aagtgattca
    12661 aatattttct tataacaaaa ataacaagca gaacaagcta caattaactg atggaaaact
    12721 cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg gaagatgaag ggtataaaat
    12781 cggtgatatt tatcagattg attcagatga attgaaagaa aaagagtata ccatcacggg
    12841 ctttgtccgt tctccattgt tcatcaacaa tttggagaga ggatacgcga acgtcggaaa
    12901 tggtagcgtc gattattttg tctatttacc tgaatccgtt tttaaaacag atatttattc
    12961 tgtcttatat cttgatttta caaacgtaaa gaatttgaat acgtacagca aagcttacaa
    13021 agataaaatg gagcaaaatc aagaaaaggc agaaaagtat ttacaagatc gacccgaaga
    13081 acgcttagaa gagctgaaaa aatcggccaa cgaagaatta gaaccggcac agcaaaaaat
    13141 agcggatggc aaagcacaaa caacgcaagc aagaacacgg ttagaagatg ctaaaaaaca
    13201 gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa gcgcaacgaa caatcgctgt
    13261 tcaaacatta cctcaacaag aagctcaact tgctgaacaa gaaaggcaat tagttgagaa
    13321 agaacgtgaa ctggcctctg cagaggaaga attgaatcga aatgcagaaa aaattgaaaa
    13381 tttaactaaa ccatcatacc tatacaatga acgttcagaa aatgtcggtt ttcaagaatt
    13441 tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt tttcctgttt tctttttctt
    13501 tattgccgca ttgatcacct ttacgacaat gacacgtatg gtagaagaaa accgcaggga
    13561 aatcgggaca ttaaaggcgt taggttatac gaaagttgaa atcgcaaaaa aatatgcaat
    13621 ctatgcaagt ctagcctcag ggataggtat tgttttagga acgatattag gaacgaatct
    13681 tttgccaagg atcatttttg agctgtctaa cgagcggtat gatattggga gtgcgcttat
    13741 tttttatgac tggccgccga ttttacaagc agctgccgca ttcttcattg ccgcatttgg
    13801 cgctgcaatg atcgttttat tcaaagattt aagagaacgg cctgctgcat tgcttcaacc
    13861 gaaagcaccg aagccaggga aacgcatttt tcttgaatac atcactccgc tgtggtcacg
    13921 ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc cgatataagt caagaatgct
    13981 tatggcgatt atcggaattg ctggatgtgc cgggctgatg gttgcaggcg tgggattgaa
    14041 agattctcta agttctgttt cagccaaaca atttggaccg atcattgact atcaagcaat
    14101 cgttacattg gattcatcca cggaagatag tcgtcaaaaa gcagcggata tgctgcagaa
    14161 gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa accgtagaat tgagaaaaaa
    14221 aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt aaaacagctg atttggatcg
    14281 ttttatccat ttaactgatc ttgaagagaa cccacgctcc ttgcctaaaa caggaatcgc
    14341 tattacgcaa aaagtagcag aactatttga cttatctaca ggagatcata tttcgcttta
    14401 tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa atcgtgaaaa actatctagg
    14461 tagctttatt tatatgtccc ctgaatatta tgcttcagtt actgatcaat cgatgatgga
    14521 taatgcattt ttgctgcgta cagataagat gacaacaaaa gaagaagaaa tgctttccga
    14581 gaaactgctt gccacagacg ctgtgacaaa tacgtctttc atgtccaaac aaatcgaaac
    14641 gcaagaagaa tcaatggcga acttagactc ggttgtctta atctttgtcg tattatcagg
    14701 actgctggct tttatcgtct tgtataatct aactaatatc aatatttccg agcgtgtacg
    14761 cgaattatct acaataaaag tccttggatt ttttgataaa gaagtcacga tgtacattgt
    14821 tcgagaaaat atcatcttta ctcttttagg aatcatcgga ggatttggtg taggatatat
    14881 attgactgac ttcatcttgc aacaagcgtc tatggagaat gtcatctttc cattagtgat
    14941 cacatggtgg gcttatgtct tatcagcagg attaactatc gtatttacgg ttattgtgat
    15001 gattgttacc catttcaaat taaagcatat cgatatgatt gatgcgttga aatcgaatga
    15061 ataaaaacag aggttgtgaa atcagcgttt tgagctaata acgagggtca gctgctaatt
    15121 gaacaccatg aatcagaggt tgtaaaaaag ccgtcctgca tcgagcaaaa agaatactca
    15181 aataaaatta gctcctcatg ttttacattt gagaagactg attgccgtag ataaaagctt
    15241 ttgaacacca tttatagaag aaacaagaat attttgctat tttaaacaag aggatagaca
    15301 aatacatgtc tgatctcttg ttttttattg ggcaacagac ctcttagaat gtataaagat
    15361 aaattttaca aacgctttct gttcagttat aataataaat gataacttgt atatataagc
    15421 tgtggtaggt aataaatcag ttgaatggag gtaaatatcc atgacattta aagacaaagt
    15481 catttaccaa atctatccca aatcttacaa agacagtaat ggtgatggaa ttggagattt
    15541 aaaaggaatc aaagaaaaac tcccgtatct gaatgatcta ggcatcgaca tgatttggct
    15601 aaaccctatt tatccaagcc ctcaaaaaga caacggttat gatatctctg attacacagc
    15661 catcgatcct atctttggta cgatggagga attcgaacag ctaatagccg aagcaaagaa
    15721 ataccggata gaaatcatgc tcgatatggt gctgaatcac gtttcgacag aacatgaatg
    15781 gttcaaaaaa gcactagcag gggaccagca ttatcaggac ttcttcattt taagagaaga
    15841 gccaacggat tgggtatcaa aatttggtgg aagtgcttgg tcgccatttg gagatacggg
    15901 gaaatattat ttgcacttat atgatcggac acaagctgac ttgaactggc ggaaccaagc
    15961 agtaagagag gaactattca aagttgttcg tttttggatg gaaaaagggg taaaaggatt
    16021 ccgtttcgat gttatcaatg ttatcggtaa agacgaacaa ttgaaaaata ataaagaaaa
    16081 tgatggcaaa cctgaatata cggacaaacc aatcacccat acgtatctaa aagaattgaa
    16141 ccaagcaacg tttgggacgg attccactat catcacagtc ggtgaaatga gctctacgac
    16201 gatcgaaaat tgtatcttgt atacagaacc tagtagagaa gaattatcaa tggtctttaa
    16261 tttccatcat ttgaaagtcg attatgaaaa tgggaataaa tggtctactc ctccatttga
    16321 ttttgcagaa ttaaagcgac tatttcatac atggggagaa aatatgagtg agggtaacgg
    16381 ctggaatgct ctatttttta ataatcatga tcagccacgt gcgctcaatc gatttgtagt
    16441 tatcgatcag tatcgaaaac aaggagcaga gatgctagca accatgatcc atttgaaccg
    16501 tgggacgccc tatatttata tgggagaaga aatcggcatg atcgacccag actattcatc
    16561 gatttccgac tatattgacg tagaaagttt gaatgcttat aagttactcc ttgaacaagg
    16621 attcacagaa aaagaagcgt ttcgtcggat caaagcaaaa tctcgcgata actctcgaac
    16681 acccatgcaa tggtcagaca cagaacaagc aggatttact acagggaaac cttggttaaa
    16741 agttggaggc aagctagaag agattaatgt agaaaaagaa ctatcttctg aagattctat
    16801 tttgtcctat tacaagaaat tgattcggct gagaaaaaca tatccaatcg ttgcacaagg
    16861 agattatcat gcttatgaag caaatcatcc ggaagtttat ggctttcttc gccaatttga
    16921 aggacagcag ctgcttgtat taacaaattt ctatgataaa gatacaagga tcgccatccc
    16981 agaaggattc acagaagcca atattcttat cgataattat tccgatactg ttcttcatca
    17041 aagtctggag ctaaaaccat accaggcaat tgctttgctg aaaaattaaa acaaaaacac
    17101 aaaacgtcgt tcctacgagc tattctcgta agatcgacgt tttgtgtttt atttaaaata
    17161 atcttcttca gaaaatgaag agttttcata ctctccttta tcgccataaa atttattgta
    17221 gcgttgtttg aatttacgga aaataaaagt aacgagtaat ttgactaacg tatagatagg
    17281 aataccaaaa ataacgccca caattcccat tagatcaccc atgacaagta aaacgattaa
    17341 gatcgtaatc ggatggatct gtaaacgatc acccattact cgaggaatca ccaagtcacc
    17401 gtggagaagt tgtacggcaa accagacaat gacaaatttg acagccataa aagtagagtc
    17461 ttggaatgca atgatcaaac ctggaaagaa ggcgataaac ggcccaatat atggaatgat
    17521 acataacaac ccagcagaaa tagctaaaac actagcaaat tctaatccga tcagtaagaa
    17581 acaaacccag tagactgctc caaggataaa tgaagcaatg atttgcccct ttagaaatga
    17641 acccaattgt tgattagcaa tctttgtcaa atttttaaaa tctgtacgaa atgctggtgg
    17701 gacaatcgct aaaactgact gataaaattt ttgaggatct ctcaaaagga aaaaagcgac
    17761 aattggacca gtaaataaag taataaaaat agctgtaaca gttgaaaaaa tgttgctgat
    17821 ccgttgtgcg ccactttccc aataatttcc aataaatcca gctatatcag acgtcacgtc
    17881 gtcaatagaa gcaaaaaatt gattaaatga atcggcaaaa ggtgttttct ccaaaaattg
    17941 tcggagactc tcttgaaaat cttgaaacaa atctggcaaa ctactgatca atgtctttgc
    18001 ctgttcttca agaaaaggaa taagccatat gcttccaagc cccaataaaa ataaaataat
    18061 cacataaacg ataatgactg acaagttcct cgaaaggtgc ttgctcaaac gtttgatgac
    18121 tggttccaaa agataataca agatcagtgc aaacaataat gggggcaaga tagtagcgat
    18181 aatgattccg acaggcttga atataaacga gatctgatgg aaaaaataaa tgatcagcgc
    18241 aagtaagata actattccta aaacatagta agaggttttt cctcctaaaa agcggatcaa
    18301 acgattcgtt gtatctgttt cttttttcac tctttttcct cctttatcta taacttcatt
    18361 atagacaaaa gcgggtataa aaggaaaatg atagaggttg attgcaaaaa tatcgtcaat
    18421 cattcatata aactttctca tcaattaaaa taagaaaacg cttgacagtt cttttaacca
    18481 tatgataaat taaatcctgt tgctaaaacg taactattac ggtttgcgaa aagcgaatcg
    18541 gaacattcaa atgaaagaag atgagaagag gagtgtcata aatgaaacga ttgataggta
    18601 tattggccat gctggtttta gcaggtatgt taactgcttg cggggcaagt gggaaagccg
    18661 aggacagtaa agaaaaattg agcgtgatga cgacctttta tcccatgtat gattttacaa
    18721 aagcaatcgt cggggatgaa ggagaagtag agttattgat tccagcaggg actgattccc
    18781 acgattatga accttctgct aaagacatgg caaagatcca agatacagat atctttgttt
    18841 ataatgatga aaacatggaa acatgggtcc ccgcaatcca aaaaacatta caagaaggca
    18901 atgtccatac gataaaagcg acagaaggta tgcttttact tccgggaagt gaagaaggac
    18961 atgatcatga ccatgaacac ggggaagaag gccatacaca tgagctagac ccacatgtat
    19021 ggctagctcc aagtttagca atcaagcagg tagcaaacat acgtgatcag ctgattgaag
    19081 cttacccaga aaaacaggaa gtatggacaa aaaatgcagc agcctatacc gagaaacttc
    19141 aagcactcca tcagctttat caagaaacct ttaagcaagc aaaacaaaga tcttttgtca
    19201 cgcaacatgc tgcattcaac tatctagcgt tagaatatgg attgaatcaa gtatcgattg
    19261 ctggtctttc ttctagtgaa gaaccgtctg cggcgagaat tgctgaactg aaacactttg
    19321 ttaaagaaca tggaatcaac tatatttatt ttgaagaaaa cgccaaagat agtattgcca
    19381 gaacgttagc aaacgaagca ggtgtatctt tagaagtatt gaatcctttg gaaggattga
    19441 cgaatgaaca aatagaaaat ggagaaaact atctttctat catggaagca aacctcgaag
    19501 cgttgaaaaa aacgacagag acagaaaacc cgctagaaga tactttgacc cccaaaaaag
    19561 aaaagagcgt ctacaacggt tattttgaag acgaggatgt acaagatcgc tctctttcag
    19621 attgggcagg aacttggcaa acagtagata cgtatgtaga agatgggact tttgatcctg
    19681 tttttgaata taaagaaaaa ttgaatcagg ataagacagc acaagaatat aaagaatatt
    19741 atacgaaggg ctatcaaaca gatattgctt caatcaagat cagtgagaaa gagatggcat
    19801 ttacgtttaa ggatggaaca acgaaacgtt caaattatcg ttatgtaggg aaagaaatct
    19861 tgacctacga ggcaggaaac cgtggcgtaa ggtatctatt cgaagcagaa gataaggaaa
    19921 gtggcgcttt tagatatatt caattcagtg atcacacgat tgcaccggct gactccgaac
    19981 attttcattt atatgcagga aatgaaagtc aagaagtatt gctgaaagaa atggagaatt
    20041 ggccaacatt ttatccagat acaatggatg gacgtgaaat tgcacaggaa atgcttttgc
    20101 actgataggc aaaaaagaac cgaagggttt ggatcggctg gtcatttagt cggtccaaac
    20161 tattcggttc tttttatgaa atcaatttct ttaatccacg gatagaggct gggatggtct
    20221 cgccgtttgc caacgtgact tctctggttt ttttattaat cgaccgcaca ttttgtgtat
    20281 tgataacata ggatttatga caacggataa aattccgatg cattttctcg atttctttga
    20341 tcttctcata gaattgtaat tgggcatgag tcaaatggac ttccagttta tgtggcgctc
    20401 ctagcgaagt agaaaaatat tggatgtctt tgatcggtaa aaatttgatc tcatcattaa
    20461 actctatttt gaagatatct ttttggattt gggacatttg aaaatcttcg aaaattttgc
    20521 tcaggatatg ctcgacattt tcttttaaat cataaagatc ttctttgacg atataatcag
    20581 tcggttcgat gttgcttttt aagattttca tcgacatttc cgtatgggaa gtcacataga
    20641 ttatttttcc taaaggatct aattcacgga tcaatttacc gatcgctaca ccatccattt
    20701 cactgtcttc tagttcgata tctaaaaagt aaataccaaa agaagaagtt ctgatcttgg
    20761 ctgcattgag aagttcttcc ggatcagagg tagaaatatg gacataactg tctaagtttt
    20821 caatcatgat tctatttttg atcaccgttg ataaaacttc taattgtttt ggatcgtctt
    20881 cacaaatata aatgggaatc atatcattac tcctttattt tatttgtata taaatcgttt
    20941 gagaaaaacg ctggttttca attgatgttt caaggaaaag ataaggattg gtctttgtca
    21001 attcatccaa gataaacaag ccaaggcctt cgttttttgg atttgttttt gttgagaacc
    21061 ctttttgttt caactggttt aacggtaatg tatttgttgt tgtattgatc acacgaatca
    21121 tcacggaatg attcttcttg agtatggtca attcgatttt tttcttcttt gcagcactag
    21181 ctccttcaat cgcattgtcc aagataatac caagcatacg tacaaggttt accgtatgtt
    21241 tcgtatccag ttcgattggc tcaggaaaat gaagctggac gatcaaccct ttttcctgtg
    21301 ccatcatgag ttttaatgac agtaaactac ggatctcagg aactttcata ttatccaaca
    21361 tacttagatt ggccggaaaa aggttcatca tctggcgtgt aggtaatatc gtttgatcat
    21421 aataagaccg taatccttct aagtcgcctt ctgaaatata gctgtgaaga gaaaaaagca
    21481 gattttggta atcatgtttg aattctctca agtcttgata gtttttttct agatctttga
    21541 tataatcctg ttgcatcctg acttgttgct ctaataactg atcagctaat ttcttattag
    21601 agattttcca ataataaacc aagatgatca ggccaacaca cagatataga atgaaaaaac
    21661 tcgtattcaa agctaagaca tcaggtgttt caccagaaaa acgggtataa aaaataatga
    21721 tatagtatgt aaataatatc aatgtcccga tcgtaccgat catacggcgg tccacagtgt
    21781 gaaaccaagt ctctaacgat ttttttaaac aaaaagctaa taaagcagat aaaaaagcgg
    21841 ataagacaat gtgaaaatac tgcaatgaat ctcctggttc aataaccgaa gatttcaaaa
    21901 caaaaaaatc gtcgatgctt gttaagtgat ctcctaaaag cgacgtcaat aacccaaaag
    21961 gaagcgcgta aaccaattgt tgacgcgaag tcgaaaagaa gaaaaaagta agcatcagca
    22021 ttcctatagc aattagagca gtaaaaatcc ctaaaatcga atagacccat ccaatcaaaa
    22081 tacaagaaat cagaatgact cccgcaaaca gatatttgta tcggatgtga tggtcaatca
    22141 ataagataca aattgtgtaa atcaaatatt gcaacgctat ggaccataaa aacataaagt
    22201 acagctccca ttgattttta ccaaattata gcacaattag cgtattattt ttttaatttt
    22261 tcagggattt tgaattcaga actcatacca aagcagcagc cagatacaaa aaagtcagat
    22321 aagttagtta attgtttaat gattgttttt ttcatgaaag attcctccaa aattcatcaa
    22381 gtagtttgta ataggaagca acgtagctat ttctaaaaca atcccaagta gcaaataggt
    22441 agaaaagtaa gaagctgaga aaaataaagt aatgagagaa aaaattagaa acatgctgat
    22501 cgccttcttt aataatgggg gacgtttctc gggttcaatg ggattgattg ctgtacctct
    22561 tgatccatag cgaagcagta acaaaaagca gctgattaac aaaaagacca tcattgggaa
    22621 ggagatagtc aattgcagat gtttcatcac caagggaacg ccccataaat aaagtagtgt
    22681 ccacagcaga caaacgaatt cagagtcgga atgcaatcca taagcatatc tgcggatgca
    22741 gatataagaa agaaaggtga ttcctgtttc cgcccatgag cctgtcacca gagaaaagac
    22801 tagaacgacc gctagttttc caagattatt caataaaact tctaaggcaa attttacttg
    22861 gacatacacc atatcttctt ccgatttgtc tccgctgaat aaggcttcta ataatttgtt
    22921 cacaacttta tctttcatct catacgtacc tccaccacaa attttatgct aaaaaaaata
    22981 aaaaacgtct tttgtttcat aaaaagtggt tttgacatca aaaccgtcat gatctcctaa
    23041 aaaaatcttc gttttccaag aaccttacca aaaaaagtga cagttttgat acgaaaagta
    23101 ctttttatga caagtttgtg ctttttcgag tgatttatgt taaaaaatta ctgaccttat
    23161 aaaaggtgat ggagggattc ttatggaaaa tgaacaattg tttgaagtcg gcgaaacata
    23221 tgcatttaaa tctgatttat ttgaatcacc aataaaagga aaaatagaaa aaatctatga
    23281 aaatagtgtg atgatacgtg tcatgaaatg cgcagtagaa gacaatgata ccgctcataa
    23341 tctgcactgg cagttagttg tacgcaaatc aagtacaata gaagaataaa aacagataaa
    23401 atgttgagag cataaagagg ttgtgagtct cccgtttagc tctgagttac aaggaataat
    23461 gttaaaaaac agcttttcat gttttatgaa tattgccttg tgaccgaggt gttggcagga
    23521 gaatactgtt tatttgaaaa aagggctgcg acaagacttt tgtcacagcc tttattttca
    23581 tgaataggag ttacttttgg ctacttttag agaagtagct tttaaggagc agataaagtc
    23641 aaggctttta tttctataaa tgaagtagta aaatgaagaa cagattttat tacaggaagg
    23701 atgccgataa ttgtgatcat tgttgcagga acgatcggtg cagggaaaag cacactaact
    23761 gaaatgcttg cccaagattt agaaacaaaa cctttttatg agaacgtgga agataatgaa
    23821 gtattgccgc tattttattc taatccagaa aagtatactt ttttattaca gattttcttt
    23881 ttgaacaaac gctttttggc aatcaaagat gctttcagcc atgatgacaa cgtactagac
    23941 cgctccatct acgaagatag tatgctgttt catttgaatg cagatttagg gcgtgtttct
    24001 gaagtagaag taaagcagta tgaaggtctt ttagaaacga tgctgaagga gttagaagaa
    24061 atttcgccac aaaagaaacc agatctactg gtttatattc gtgtatcctt tgaaactatg
    24121 ttagcgagaa tcaaaaaaag aggccgggaa tatgaacagc tagaacaaga tcctgaactc
    24181 tattcctact ataaagaact caatcgacga tacgaagagt ggtatgaaca gtttgatata
    24241 tgtcccaaaa tagtgatcga tggggacaaa tatgatttcg tagccgatcc ggcatgtgga
    24301 gaacaaatcg tacaagaaat caagatgcgg gcaaagaaaa tgacagaaga ggcagatgcc
    24361 cagtcgatcc gctacactac aaataacccg aaagcaaagg gaatctttac aaataaataa
    24421 tataaaaact aagcaaattg ctcactcgca gatatttctc tgtgagcagc atcttgctta
    24481 gtttttttga ttcattgaga tcaaatagtg aaccgattcg tatgataaaa ttgccataaa
    24541 tgaaatgtct gagtgagggc catgatcaaa aataaaatgg aaacgatcaa aatcgaatgg
    24601 gcgccaaaca gatgatgaca gaaaacaagg acaatagctc ctgcgataaa ggcaagaacg
    24661 acacttagat agtggaaaaa tcgatcaaaa tctgcttttt ctttagaaat acaataatca
    24721 tataagttcg ttgctacccc ttttaaattt ccagttgtaa ataaattagt gtaggcacgc
    24781 ccgttgattt tatcaaaagc tacccactgg atcgatgcag taaaagcaat caaaatcgtt
    24841 actaatgcgg gatgctcatg gacgggtgtc aaactaacaa agaaaaaaat cactgcttca
    24901 taatatagaa caaacaagcg ccagaaatgt ttttcttttt ttctaaaata atcaattaga
    24961 tactttgtta gaagaatccc taagaaaaag aaaagggtag acaatacttt tttaccaaca
    25021 cttagccatt ctccgtcaaa tgcttggatg atcgatagaa tgatattgcc agtttgagca
    25081 gaggcaaaag caccatattg gatataagta taactgtcca tggctcctcc gataaatgtc
    25141 agcaaaagtc ctattgtacg cccctcgtga aaggaagtct gcattttttt catcaccttt
    25201 tatgaatatt tttgtcttcc ttatcttagc ataaacgatt gaaaaaaagg aaaaattttc
    25261 agaaaactaa aatcatatcg cttcccattt tcatttttag aaaggtctgt tagtataaag
    25321 aaaaaataga caacgaaact ttaaataacc aacttgaaat aaacaaattc aaagaagaaa
    25381 gatgggaagg aaatcaatca gttgaatgaa aaagaaattc tccgattaca aaaagaattg
    25441 aaacgctggg agaacaagta taaaaagctt caacaagtct ttcggctaca agaagaaatc
    25501 attgctacct atgaagcggc ttatggcgaa gcacccggac ttattgagga agaagtacaa
    25561 gtaaaagaaa gtgaaggaaa cagctctttc tttaaaatga tggagaaacg ttcctataat
    25621 cgcttcaatc aagaacaaaa agcagctatc acatatgata tgaagaaaca tcttcgaatc
    25681 atagcaggag ctgggagcgg caaaacgcaa acaatctgtg caaaagctgt ttatctgatg
    25741 acgcaaaaac aagtagacga agaacgcata ctaatgatca cttttacaag gaacgctgca
    25801 aatgaactaa aaaaacgagt agataatttc agccagagaa aaaccgctgt ccatatcgga
    25861 acattccata gtatcttttt taggatatac aatgaaattt gtcgaaaatt tcctgaagta
    25921 gcaatgcaag gaatacaagg agacttttca aaggatactg cacaaaaagt gaatgctgtt
    25981 ttgcaacagc tgatacgtaa atataatctt tatatatttg atcaatatgg agaaaaaacg
    26041 attgcttctc ggttagacta ttggcagaat atgaaccttt cagtagaaga aatggttcag
    26101 ttagttaaag aaaaatatga ttccattgat aagaatgcac gacagccgat atcagaacga
    26161 ctgtatggat tgctcacgga actccaagaa caaaaaagaa gccagcaatt gctagagttc
    26221 aatgatgtac tccaaaatct gaaaagcgct ttggagaatg aggagatcca gcattatatc
    26281 ggccaaaaat atgattatct attcattgat gaatttcaag atactaatcc acttcaatgg
    26341 gaaatcgtga aacggatgac aaaggaaaac aggatcaagt tgattgttgt tggagatgat
    26401 gatcagagta tctatggatt cagaggatca gagccagcat acatcaagaa ctttgaaaaa
    26461 gaatatccta ccaaaactct tttcttattg actaattatc gttctcgggc agctattgtc
    26521 caaggagcaa atagattgat cagctataat aaaaatgatc ggataccaaa ggcgatgatt
    26581 ccagcacaaa aagaagcagg tgttatggaa gcctatttgt tttctgatac taaagcagaa
    26641 agtcagtggc taatcaaaca aattcagaca tttattcaaa aaaatgggaa atataaggaa
    26701 tcaatcatat tataccgatc agcttcacaa acgcagcagc ttgtccaatc tctgctgaaa
    26761 acgaatattc cttttgtact ggaagcagat agtccatatg aaggtatctt tggtatcaag
    26821 tctttccaac atttctatca aaaaattgtt cattggcaaa cagcactgaa tctgcaaaag
    26881 aaaaatcagg cctaccagca attgctccgg cagctgatgg cagactgcta cctgaaaaaa
    26941 agtgaatgcg atcaatactt ttctcctaaa tatcaaaata tagccgttgc agattatatt
    27001 ttgtcgataa gccccaattt aaaatcgaaa tctgcagaaa tccgagagtt tgaggaagcg
    27061 ttgcgtaaaa cagaccagca tcaagaaatc tattcgcttg ttcaaagcta tcttcatctg
    27121 ccacgtatag ctaaggaaat cgaccagagt gatcaagaat ggatacgaga agaagtaaag
    27181 cagcatgcta ctttcagaag actccaagcc ttatcgcaag aaacccaagc gacagctaaa
    27241 gaattaaaaa cccgcttgat tgcttacaga caaggaaaac ttgatgcact atgtatccag
    27301 agcattcaca aaagtaaagg tcttagttac cgaaatgtct ttttgattgg ttgtaacgaa
    27361 ggaagtttgc cttataacgg agcgacagaa aagaaagtaa tcgactttaa tgaaataaaa
    27421 gcagaaccag tgacgacgat agaagaagaa cgaagactat tttatgttgc gatgactaga
    27481 gcaagaaatc gtctgtatct atgtgtacct caaatgaaaa attcaagaaa attgaaagta
    27541 tcgagattta tcaaagaaac cgggagtcgt gtgaaaaaag gaaagtgata gaagagaggg
    27601 tgtgacagaa gtggtcagtt tcaagaaaga agaaggaatg tacgaaaatt gcttctcaaa
    27661 tttttgtgaa atttcagctt attttcgaag aagctatttc tggaacacag tttattcgga
    27721 aaagggaggt gtgacaagaa ttttgtcaca cctcccttat ttattttgaa a
//
LOCUS       DASOUD010000029        25957 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.029, whole genome shotgun sequence.
ACCESSION   DASOUD010000029 DASOUD010000000
VERSION     DASOUD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.029"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..288)
                     /locus_tag="U4A29_001137"
     CDS             complement(13..288)
                     /locus_tag="U4A29_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO0680273.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(531..1841)
                     /gene="der"
                     /locus_tag="U4A29_001138"
     CDS             complement(531..1841)
                     /gene="der"
                     /locus_tag="U4A29_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO0680274.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2023..3252)
                     /gene="rpsA"
                     /locus_tag="U4A29_001139"
     CDS             complement(2023..3252)
                     /gene="rpsA"
                     /locus_tag="U4A29_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO0680275.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3387..4067)
                     /locus_tag="U4A29_001140"
     CDS             complement(3387..4067)
                     /locus_tag="U4A29_001140"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO0680276.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4116..4751)
                     /locus_tag="U4A29_001141"
     CDS             complement(4116..4751)
                     /locus_tag="U4A29_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO0680277.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4817..6220)
                     /locus_tag="U4A29_001142"
     CDS             complement(4817..6220)
                     /locus_tag="U4A29_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEO0680278.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6207..7238)
                     /locus_tag="U4A29_001143"
     CDS             complement(6207..7238)
                     /locus_tag="U4A29_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0680279.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7269..7490
                     /locus_tag="U4A29_001144"
     CDS             7269..7490
                     /locus_tag="U4A29_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO0680280.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7655..8392)
                     /locus_tag="U4A29_001145"
     CDS             complement(7655..8392)
                     /locus_tag="U4A29_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEO0680281.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8545..9885)
                     /locus_tag="U4A29_001146"
     CDS             complement(8545..9885)
                     /locus_tag="U4A29_001146"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEO0680282.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10122..11645)
                     /locus_tag="U4A29_001147"
     CDS             complement(10122..11645)
                     /locus_tag="U4A29_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO0680283.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12023..12634)
                     /locus_tag="U4A29_001148"
     CDS             complement(12023..12634)
                     /locus_tag="U4A29_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO0680284.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12757..12882)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12964..13680)
                     /locus_tag="U4A29_001149"
     CDS             complement(12964..13680)
                     /locus_tag="U4A29_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO0680285.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13686..14255)
                     /gene="scpB"
                     /locus_tag="U4A29_001150"
     CDS             complement(13686..14255)
                     /gene="scpB"
                     /locus_tag="U4A29_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEO0680286.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14239..15036)
                     /locus_tag="U4A29_001151"
     CDS             complement(14239..15036)
                     /locus_tag="U4A29_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO0680287.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15006..15377)
                     /locus_tag="U4A29_001152"
     CDS             complement(15006..15377)
                     /locus_tag="U4A29_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO0680288.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15396..16283)
                     /gene="xerD"
                     /locus_tag="U4A29_001153"
     CDS             complement(15396..16283)
                     /gene="xerD"
                     /locus_tag="U4A29_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO0680289.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(16379..16852)
                     /locus_tag="U4A29_001154"
     CDS             complement(16379..16852)
                     /locus_tag="U4A29_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEO0680290.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(16957..17820)
                     /locus_tag="U4A29_001155"
     CDS             complement(16957..17820)
                     /locus_tag="U4A29_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO0680291.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(18016..20115)
                     /locus_tag="U4A29_001156"
     CDS             complement(18016..20115)
                     /locus_tag="U4A29_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEO0680292.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(20335..21444)
                     /gene="rpoD"
                     /locus_tag="U4A29_001157"
     CDS             complement(20335..21444)
                     /gene="rpoD"
                     /locus_tag="U4A29_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO0680293.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(21468..23309)
                     /locus_tag="U4A29_001158"
     CDS             complement(21468..23309)
                     /locus_tag="U4A29_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO0680294.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(23491..25806)
                     /locus_tag="U4A29_001159"
     CDS             complement(23491..25806)
                     /locus_tag="U4A29_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEO0680295.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
BASE COUNT         7084 a         5603 c         4410 g         8860 t
ORIGIN      
        1 ttttcaactg tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac
       61 tttgctagct ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc
      121 gcgttcgcga acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa
      181 agcatcttga atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa
      241 atcagtagct gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac
      301 ctcctccaca agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga
      361 agtttaaaaa gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg
      421 cttaatagca aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa
      481 acgaaactgt ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc
      541 ctacgaggga taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt
      601 tctaagaaac gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat
      661 gtaggtggtt tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct
      721 gttggtgttg gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag
      781 atacgaagat tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt
      841 tgtttagtca ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca
      901 cgaatctctt gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta
      961 ttcacgacga taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct
     1021 tgttcccgaa tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg
     1081 gcacgcattg cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta
     1141 cgcataccag ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg
     1201 tcgattgcgt ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa
     1261 atcgcattga tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc
     1321 gtgtcgtcgt cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata
     1381 tcaccaatcc ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa
     1441 taaaattcat agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc
     1501 actggtttat tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct
     1561 tcgcgtccgc tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga
     1621 tgcttgatct gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg
     1681 ctgaattctc gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga
     1741 gtatcttcga caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg
     1801 acgttcgggc ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag
     1861 tttccaatct cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt
     1921 aaacgaagta tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga
     1981 caaaagcagc tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc
     2041 ggttgctttt tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa
     2101 tccagtattt tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc
     2161 tggtttttct tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc
     2221 taatactttc actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg
     2281 tttatgtgaa atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa
     2341 ggcaccgaag cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt
     2401 tgtatcgatt tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc
     2461 ttgttcagga ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc
     2521 acttggtttt gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc
     2581 gcctaggtcg acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc
     2641 tccctcatgg atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac
     2701 cgctttatgc gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct
     2761 caatgttttg cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc
     2821 agggacaaaa ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac
     2881 aggtgcttcg atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt
     2941 agcatctaaa cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt
     3001 actgataacg actaaatcta atacatcacc gacttttact acttcgctga tatcttctac
     3061 cggcaaagtt gataattctt ttgccggaac gactccttca actccagccc cttcgattcc
     3121 gacaatcact tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt
     3181 tacttcttgt acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc
     3241 aaattctgtc ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt
     3301 ttagtttaat tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac
     3361 aattgttttt catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca
     3421 gcttttattg ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca
     3481 tcctctgctt gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt
     3541 atctctttcg tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa
     3601 cgtctttctg cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt
     3661 aatacagctg tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt
     3721 cgttggacag caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct
     3781 tttgtgacat ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc
     3841 tgtccatctt cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct
     3901 tcttcatctg aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg
     3961 gcaccggtat ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt
     4021 ttcccagatg aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt
     4081 tttctcagaa aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca
     4141 gggtaaagca taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg
     4201 atccctgcac gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa
     4261 ccggctgcag ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct
     4321 tgataagttg cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac
     4381 gttacacctg tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt
     4441 gaagtctcag ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa
     4501 gtttcagaag aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa
     4561 gcacctgctg gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat
     4621 gtatttcctt tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg
     4681 tagattggtt gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct
     4741 tttctgctca ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt
     4801 gtcttgtcct aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttt
     4861 cccaatgaag ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg
     4921 ctccatcaat atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta
     4981 agatgaattt tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga
     5041 attctttttc tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact
     5101 gctggatcag ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat
     5161 attccaaata aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat
     5221 ggatacgttc atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc
     5281 ctgctcgacc gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca
     5341 cataacggat atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact
     5401 gcagctgatt ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac
     5461 cgtgatagta tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt
     5521 tttttgttgc acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt
     5581 cttgttcttt ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt
     5641 gtctgatctc ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat
     5701 ctgttgctgt cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc
     5761 ctaactgttg atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat
     5821 ctactacata taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca
     5881 acatttcggg actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc
     5941 tttcagattg cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta
     6001 attgcgtcac ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt
     6061 ccattaaata ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag
     6121 cgagagtatc ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg
     6181 aatcaaatcc aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc
     6241 aatagataga tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat
     6301 tcgctaaagc gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct
     6361 gcagagagaa tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga
     6421 tctgttactg ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca
     6481 ttcataaaca tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa
     6541 tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg
     6601 atacatctgc tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa
     6661 ggaagcagcc ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct
     6721 tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca
     6781 cgtggcaatt ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc
     6841 tctgctggaa gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat
     6901 ttaatgagcc gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta
     6961 tgttctattt gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt
     7021 tcatttattt cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc
     7081 tgcttcaatt ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata
     7141 agtactgaag tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg
     7201 tgtcctgatg aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat
     7261 ggttagaaat gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt
     7321 gtcaaatcga agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc
     7381 aagaaccaga ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag
     7441 cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga
     7501 aactttaaaa acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct
     7561 tgttccgtga aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact
     7621 agttttttca cggaccctat aggacccatt tatattattt caactttttc agccagtttt
     7681 ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc
     7741 ctttttgctc accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact
     7801 ctgttctttc cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat
     7861 cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt
     7921 cgggataact tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga
     7981 cataacgact tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg
     8041 gaaaatcttc tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca
     8101 ttttgacaat agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac
     8161 tttctggctc tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct
     8221 ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt
     8281 gcgcttctga cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga
     8341 gtaatcctaa aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc
     8401 accttttcta tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat
     8461 aagagagaaa tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata
     8521 tccgcccttt ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata
     8581 aatttccatt gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa
     8641 gtcaggagta tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa
     8701 agtagcgccg cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc
     8761 caatagattt ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa
     8821 ttcttcataa gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc
     8881 tttcttgaat gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat
     8941 ttccaaactt gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac
     9001 gacagcttca ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa
     9061 atcaaatact tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc
     9121 atctttcgcg tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc
     9181 gaattcgtgt tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc
     9241 tgcatcagga acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg
     9301 tcctgtcaag taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc
     9361 tggcgcaaat ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca
     9421 taaattttct tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc
     9481 atcaatcaaa gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga
     9541 tccgcgacca actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt
     9601 attttcgtcc cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc
     9661 agagccaatc tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag
     9721 cttttcactg atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg
     9781 tccttgtcct gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga
     9841 ttctaatgct tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta
     9901 atatgttaaa aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga
     9961 cgttttaagc ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac
    10021 actcactgaa tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc
    10081 ttcattctta tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca
    10141 cctaggatca cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac
    10201 cacactgaaa catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt
    10261 gtaattaagt ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg
    10321 acgccggcaa attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa
    10381 gttgaaggaa tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca
    10441 cttgaagata atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat
    10501 ccattgttca taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc
    10561 aaatcagaag caccagctaa aaattcagag acaaattcag actctttcat gccataagtg
    10621 attgctacta cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca
    10681 cctaatggaa gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag
    10741 tttgtcaatg attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg
    10801 atgatcatga taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg
    10861 attacttctt gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg
    10921 ttgtatacta atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat
    10981 acaatagaaa tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc
    11041 atcgaaatcc caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct
    11101 agacagccta cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct
    11161 acagccatca tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg
    11221 taagtagagc cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt
    11281 cctttgaatt tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata
    11341 atacctaaaa atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt
    11401 tctttagtta gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata
    11461 tcccacagcc cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct
    11521 gcatcgtcta cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca
    11581 ataataataa ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt
    11641 tccatcttct tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt
    11701 tatcaaaaag agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa
    11761 aagttcgttt ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc
    11821 attgtagcgt ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt
    11881 tcacatttgc aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa
    11941 caaataaaac gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga
    12001 aatttcttga tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt
    12061 gttttcgact taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga
    12121 tgaatccttt gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca
    12181 tgcccagcat ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga
    12241 aataatttgc gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa
    12301 atttgtttct tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa
    12361 agatcgttgt tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg
    12421 tagtcagcag atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga
    12481 ataagaacat gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag
    12541 ccggcatgat cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta
    12601 ctgcaaccat tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc
    12661 ttcagcgaac tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata
    12721 gagttagagt tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat
    12781 actgtcggta tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta
    12841 taaccaccgg tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt
    12901 acatattcat tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag
    12961 tgttcatttt gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc
    13021 cccaggacgt aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac
    13081 aggaagacca actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt
    13141 caattccacg atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg
    13201 agatgttttt cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt
    13261 agcgatccct tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag
    13321 gcgctgagca aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag
    13381 tcgacctaca ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct
    13441 tcctttgtca tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc
    13501 gtattcttct tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt
    13561 tatccctaat tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc
    13621 ttctgctttt cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat
    13681 tacaattatt ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca
    13741 agtgaaattt ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa
    13801 tctaagaagt agccagttgt tccatataat atcggccgac ctggtccttc tactctacct
    13861 ttttcttcaa tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct
    13921 cgaatctgct cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt
    13981 tcgacggcgg cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga
    14041 gccagctctt ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct
    14101 gagtgcgagt tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct
    14161 tcgctgagat ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata
    14221 aaaagaaggg cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata
    14281 aagcataatt gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa
    14341 ttctagcaaa gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga
    14401 atcgaaagta cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc
    14461 ttcgatcgtt gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg
    14521 ttctaagatt ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa
    14581 cggcttttca gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact
    14641 tcgttcctct gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa
    14701 aagctgagtg accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgac
    14761 gaattcttgg acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac
    14821 taagtattct cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata
    14881 ttgctccgtg atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag
    14941 atgaagcagc agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa
    15001 ctcattcact ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt
    15061 tcagttgcac ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag
    15121 cccatattct caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg
    15181 ttcgttgccc agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag
    15241 aaaaaaagtt cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt
    15301 ttacctgtca gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc
    15361 cggtaatgtg ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta
    15421 taaacatccg tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc
    15481 gcgtgaccta acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg
    15541 gcaaagctat gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact
    15601 aactgcttca gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca
    15661 aatacatgca tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt
    15721 tccagccatt ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca
    15781 actgtctgta ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca
    15841 ctgacgcgca tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac
    15901 gtttttgtcg tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt
    15961 ggtaatttct gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata
    16021 cgctcttgcc gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt
    16081 gctgtcgctt ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg
    16141 acatcctgcc atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca
    16201 cgttcatagc tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga
    16261 taatcgttga tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac
    16321 cgcttcattc tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc
    16381 agacggactg gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta
    16441 gccgatgatc ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta
    16501 aaagatcctc ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga
    16561 aatgtttggc gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt
    16621 tatcgacaat tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa
    16681 tttccggact cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct
    16741 cattttccaa aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt
    16801 catgtagctg ctgttttgtt tttttcatcg ccgaaatagc attcatgacc atttactcaa
    16861 ccccaacaat ttataataat tacaaataaa aaaagatata acactatgtt aattaaaatt
    16921 attataagct agttaagaaa aacatgcaag ggattctcaa ttagattttt tgactagttt
    16981 tgtcacaccg ttttcttgtg taatcaatcc ttgtttcaaa agattaccta atgcgcgctt
    17041 aaatgctcct ttgctgatac caaaagcttt catgatttct tctggatccg atttatctgt
    17101 aaagttgatt tgctgatctg cagaacgttc taaaatggtt aaaatcattt gtgcatcatc
    17161 tgatatagct tcatatgctc ttggcttcaa tgaaagattc agtacaccgt ctggtcggac
    17221 accaatcact cgacctttta tctgctcgcc tagacgaggt tcattatatc gttcagaagg
    17281 atggataaac ccaagataga aatcttccgt taaaacgtac gtacctgtta atttcaagcg
    17341 gtatgctgtt cctatcacat ctttgttttt catttcttct gttccatatt tgcttaaaga
    17401 tttgaatatt ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat caactctaag
    17461 ggaaatcatc aagcgatccc cttttttagg ccatagctct gtcattgtcg gcaattcatc
    17521 aagagatacg acaaagtctt tatctggaag accgatatcg acaaacgcac ctaaatcttt
    17581 tcgtgtagca gtaacgattc caaaaccgta atgaccgata cggcttttag gaatttgtgt
    17641 agtgatccga ttttcatgtt tttgattttg gtaaccgaat ccttcaactg cttcaccgat
    17701 tttgtgttca ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta
    17761 gtttttttca ttttcatcaa taataatacc tgtaaatata gttgctaata aatcgttcat
    17821 ttttttctcc ctctatagtg aaaggttttc ttaatacatt tctttgctat cataacataa
    17881 tgttatctac ttgaatagct gagccttggt ttggaaaggt gaaaaaagga gtatgacaac
    17941 agttgtgtca tactcccctt tttcgaataa acggtgttaa acggcaggct cacagcctct
    18001 ttcatttgcc taaaattaat tgctcccatt tcctccgaac catccagttt tacagttgat
    18061 ggttacgatg taaaatggca aagcactttc tccaatgcct ttcatcaatg cagattttgt
    18121 tgaagtggca tatagattat aatagccttc cccattttcg attctggctt tttctaatct
    18181 gtaacctcct tcaacgatat atccacgttg gatacatgta gcaatcactt tgctttggac
    18241 acctggtgcc cacgtccatg ctggattaga tgcatcggca atatcacttg ctctggcaga
    18301 tttgattggc tgattcgctg agtttgaaga ggaaggggtc gaggatgccg cttcttgctg
    18361 tgctgcttgt gcgaccgcag cttgttgagc ggcttgagct gcagctgctg cttgagcggc
    18421 ttgttttgct tgttcttcag ctgctttttt cgcttcttct gcttttttct tttcctcttc
    18481 ttcttttttc tctttatcag tgagcatctt atctacttgt ttcaattgtt cattcaactg
    18541 agcagataga tctgtatttt tcactttttc tactgcttct tttgccgtct tgtattggtc
    18601 gcgtgttgct gaatccacta cttttccgtc tttaaagact atagctgttt tttctttagc
    18661 tgtttgcaat tcagcatatt gatttttagc ttctgtttgt gcttgttgaa tcaattttcc
    18721 aaaatctgtg ttatccgttt cgattgcttg gatctcttta tctgctttca acttgatgtc
    18781 atctgctaga tgatcatcga cgatggcagg tgctatgaac cactcattca ctttttggat
    18841 atctcggatc ttagattgga tatcttggta agttgtttct aactctgaat atcctttttc
    18901 attcttaaga ctggataaat cattttttag ttttgataaa tcttgatgaa tcatatctgc
    18961 acgaataaat tgatggtgat catctgtata aaaggcagca agtttctcat gaagttcttc
    19021 catttgttgc tgtctttttg cttctgctgc tacttcggct tctacttttt gatgatggtc
    19081 ataataagcc cagcttcctc caccgagtag aagtactgct gctaaaacga gataaggtac
    19141 tttttttgat tttttctttt gcgctttatc tggatctgtt ttttctaagt ttgattcttg
    19201 atgtgttttt tcagtatctg gcacgattgt tattgatttc gcttctgctt caaacagctt
    19261 aggctggttc tcttcttttt ctatttttgc ttcaagatca gtaccctctt tgcttacaga
    19321 ttgattttca gcatcggatt cactatttat ttcatcgggc tctgttgact ttttgtctgt
    19381 gttttcgagt atttcttttt tctctgcttc cggcagttta ttcgtttttt ccttttctaa
    19441 ttgtttttca gtaaatactt cagttttttc tgtgatatcc gtttttacgt cttttatttt
    19501 tactgtttct ttacctgtgg cgttttctct atcagagtct ttttcagttt cttgttcctt
    19561 ttctggctct actttgtttt cctgtgcaat tttttcactg atcattttga gcagctcttt
    19621 cgcgtgctct tcttttgtat cttcacgatg tgctttgata taagcggata gtatcgaatt
    19681 ttcttcctca ttatcgacga tttctttcag ttcagctact cttctttttt gttttgattc
    19741 aagcgtttct tcttgattct ttcctgattc ttccgaagtc tcatgaacag ttgctttctt
    19801 ctgttcattt ttctctatat tagttacttg atcgtgagca ggtggttctt cgtcttctgg
    19861 ttctgctttt tcgaataatt ccattacaga ttctagaggt aaatctttca attccgacca
    19921 ttcgattgga tcgtttaatt ctgtatcagg aaagactgca tcttcagttg atttatcctg
    19981 catagctgca tctgtatttg tttcttcaat tgattctttt atttgctgat cttctgcagt
    20041 tatttgatga ccgcagtctg gacagatagt attctgctcc atttttttcg atccgcaatt
    20101 tggacatttt ttcatcactt agcgatcggc tcctttacta ggtttacgct accatttaat
    20161 ctatcatatt ctatacgtca cgccaatagc ttatttttag gatttactta aaaataatga
    20221 atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag ctttttttct
    20281 tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc acgcttattc
    20341 taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta atgctttagc
    20401 ctcgatctga cgaatacgtt cccgagtaac accaaacact ttccctactt cttccaatgt
    20461 tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt cccgatcagt
    20521 caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg cgtgttctgc
    20581 tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat cttcttcacc
    20641 gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc ggactttttc
    20701 tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc caaggtcttg
    20761 cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat gaactggaat
    20821 acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt
    20881 agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg ccttcatcag
    20941 tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta cataacgttt
    21001 agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc
    21061 gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa gggagacacg
    21121 gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga
    21181 aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc tgtgaattga
    21241 tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt ggatcaattt
    21301 gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata aatcatcata
    21361 tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg cttcatacgt
    21421 ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta tttggcttgt
    21481 tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag ctgtttgttt
    21541 ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc agctaagcca
    21601 gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc ttctgccacc
    21661 ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa aaagtctaaa
    21721 aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa aaaataaact
    21781 tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt acggacagaa
    21841 acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg cgtcaaaggt
    21901 ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc ctgaggttct
    21961 tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg
    22021 ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat tggtgaaaca
    22081 actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt ttcattatct
    22141 aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc gcgcccatga
    22201 gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc aggatcaagc
    22261 ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag caattcaatc
    22321 cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac caattctttc
    22381 gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat agaagctaat
    22441 ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc gaacaacaaa
    22501 atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa aacttgtctt
    22561 ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt ttcctgccct
    22621 ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt
    22681 ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg cgtgacaaac
    22741 aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac ttgctgaaga
    22801 aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc taaagacaac
    22861 ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa tagcatgtga
    22921 tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc agattcagga
    22981 ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg ttcgaagtct
    23041 gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag aaaagtaaac
    23101 acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa
    23161 aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt accagacttt
    23221 ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg tactgtttcg
    23281 ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt gatatctttt
    23341 gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta actgaaatca
    23401 gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca ttgctttttc
    23461 aagtataaca catttctatc tcattaagca tcaatttgct actcttttca gtaactgatt
    23521 ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca tgaatacaaa
    23581 gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat ggaacagcat
    23641 tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca atgggttgaa
    23701 gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt aatacatcaa
    23761 agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca ccgcaatgat
    23821 attcactact acaagcaagg catttggtaa agcattaata agtgctgtag gcaaataacg
    23881 gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat acccttcaat
    23941 cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg ctgtaacggc
    24001 acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc ctgagacttt
    24061 cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg tagtaaaatc
    24121 agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt ctgccatagc
    24181 aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc
    24241 aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct gcggactcac
    24301 tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt cagtaatttg
    24361 agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga tattggagac
    24421 agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat gaagataagc
    24481 tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag caaccggctc
    24541 aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca acataagtac
    24601 gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt cttccaccaa
    24661 acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg cgccccattt
    24721 gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa gctcaaaata
    24781 atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat acgtccccat
    24841 aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct ggatactcat
    24901 tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat gggccagcgt
    24961 ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg ttacccctgt
    25021 cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta acaaagctgc
    25081 tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg cttccaaaaa
    25141 tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt tgcgaatcga
    25201 atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca agcggacagc
    25261 ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg tcaaaaagct
    25321 tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag
    25381 agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta tctgttctcc
    25441 tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg attgttcttt
    25501 accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc gtacaagatt
    25561 tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa ttgctaaata
    25621 cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa agttcagaaa
    25681 attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg atgcatcttc
    25741 ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct gtgtaagtcc
    25801 attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt gtttaagtct
    25861 agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa acacacgaat
    25921 gattttacaa aaaagatgcc aacctttttg tttgaat
//
LOCUS       DASOUD010000030        25943 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.030, whole genome shotgun sequence.
ACCESSION   DASOUD010000030 DASOUD010000000
VERSION     DASOUD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.030"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..212)
                     /locus_tag="U4A29_001160"
     CDS             complement(<1..212)
                     /locus_tag="U4A29_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEO0680296.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLV"
     gene            478..822
                     /locus_tag="U4A29_001161"
     CDS             478..822
                     /locus_tag="U4A29_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEO0680297.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            823..1242
                     /locus_tag="U4A29_001162"
                     /pseudo
     CDS             823..1242
                     /locus_tag="U4A29_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1334..1684)
                     /locus_tag="U4A29_001163"
     CDS             complement(1334..1684)
                     /locus_tag="U4A29_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680298.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1650..2981)
                     /locus_tag="U4A29_001164"
     CDS             complement(1650..2981)
                     /locus_tag="U4A29_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEO0680299.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3237..3803
                     /locus_tag="U4A29_001165"
     CDS             3237..3803
                     /locus_tag="U4A29_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO0680300.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3948..4035
                     /locus_tag="U4A29_001166"
     tRNA            3948..4035
                     /locus_tag="U4A29_001166"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3980..3982,aa:Ser,seq:cga)
     gene            4369..5343
                     /gene="bsh"
                     /locus_tag="U4A29_001167"
     CDS             4369..5343
                     /gene="bsh"
                     /locus_tag="U4A29_001167"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEO0680301.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5668..7563
                     /gene="asnB"
                     /locus_tag="U4A29_001168"
     CDS             5668..7563
                     /gene="asnB"
                     /locus_tag="U4A29_001168"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEO0680302.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7758..8204)
                     /locus_tag="U4A29_001169"
     CDS             complement(7758..8204)
                     /locus_tag="U4A29_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEO0680303.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8432..8581
                     /gene="rpmG"
                     /locus_tag="U4A29_001170"
     CDS             8432..8581
                     /gene="rpmG"
                     /locus_tag="U4A29_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO0680304.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8669..9187
                     /locus_tag="U4A29_001171"
     CDS             8669..9187
                     /locus_tag="U4A29_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO0680305.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9264..10220)
                     /locus_tag="U4A29_001172"
     CDS             complement(9264..10220)
                     /locus_tag="U4A29_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO0680306.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10386..11192
                     /locus_tag="U4A29_001173"
     CDS             10386..11192
                     /locus_tag="U4A29_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680307.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11189..12178
                     /locus_tag="U4A29_001174"
     CDS             11189..12178
                     /locus_tag="U4A29_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO0680308.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12175..13179
                     /locus_tag="U4A29_001175"
     CDS             12175..13179
                     /locus_tag="U4A29_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO0680309.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13311..13853
                     /locus_tag="U4A29_001176"
     CDS             13311..13853
                     /locus_tag="U4A29_001176"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO0680310.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13873..14571
                     /locus_tag="U4A29_001177"
     CDS             13873..14571
                     /locus_tag="U4A29_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO0680311.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14592..14804
                     /locus_tag="U4A29_001178"
     CDS             14592..14804
                     /locus_tag="U4A29_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO0680312.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14804..15766
                     /locus_tag="U4A29_001179"
     CDS             14804..15766
                     /locus_tag="U4A29_001179"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO0680313.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15784..16179
                     /locus_tag="U4A29_001180"
     CDS             15784..16179
                     /locus_tag="U4A29_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO0680314.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16342..16707
                     /locus_tag="U4A29_001181"
     CDS             16342..16707
                     /locus_tag="U4A29_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEO0680315.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16704..18515
                     /locus_tag="U4A29_001182"
     CDS             16704..18515
                     /locus_tag="U4A29_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO0680316.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18577..18744)
                     /locus_tag="U4A29_001183"
     CDS             complement(18577..18744)
                     /locus_tag="U4A29_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO0680317.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18992..19981
                     /gene="galE"
                     /locus_tag="U4A29_001184"
     CDS             18992..19981
                     /gene="galE"
                     /locus_tag="U4A29_001184"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO0680318.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19993..21477
                     /gene="galT"
                     /locus_tag="U4A29_001185"
     CDS             19993..21477
                     /gene="galT"
                     /locus_tag="U4A29_001185"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEO0680319.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21501..22481
                     /locus_tag="U4A29_001186"
     CDS             21501..22481
                     /locus_tag="U4A29_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0680320.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22672..23394
                     /gene="tsaB"
                     /locus_tag="U4A29_001187"
     CDS             22672..23394
                     /gene="tsaB"
                     /locus_tag="U4A29_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO0680321.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23378..23920
                     /gene="rimI"
                     /locus_tag="U4A29_001188"
     CDS             23378..23920
                     /gene="rimI"
                     /locus_tag="U4A29_001188"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO0680322.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23927..24391
                     /gene="rimI"
                     /locus_tag="U4A29_001189"
     CDS             23927..24391
                     /gene="rimI"
                     /locus_tag="U4A29_001189"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO0680323.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24388..25404
                     /gene="tsaD"
                     /locus_tag="U4A29_001190"
     CDS             24388..25404
                     /gene="tsaD"
                     /locus_tag="U4A29_001190"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO0680324.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8400 a         4447 c         5432 g         7664 t
ORIGIN      
        1 acaagcgggc gtctaccaca gtttgatttg tttttcttat actgttgata gtactctaag
       61 gctgattttc cttgcttgaa gaacaggtat actttatgga tcgtttgtct tgaacgattc
      121 aagaaatgcg cagttttcgc aacagattga tttattttga aataagactc tatcattaca
      181 agttcatccg ttgtaagatg ggtataggtc atttgtactc actccttata attctttgga
      241 cggaactatt ttgagtgtag cacaaatgac ttttttagtt gtctagctta attttacaat
      301 cggcgaataa aaaaagcgtt agcaactatg cttaaaaagc atatcgactg aataaactaa
      361 gcattggaca ggctgaaaga ctacatttaa aatgaaatgc gaagaaacaa tagaaaataa
      421 agttgcctta gaaccatagt tgaatcacct aacagtcgtg aagggagaat aagagaaatg
      481 gcagaaaaac aaaccgcagg acgagatcaa ttaggagatt ttgcaccaaa atttgcagaa
      541 ttgaatgatg atgttttatt tggacaagta tggtccagag aagaattatc tgctcatcag
      601 cgtagtatca tcaccatatc tgcattaatt tcaggaggaa attttgaaca attgccagct
      661 catctgaaga ttggcaagca aaatggcatt actagtgaag aaatagcaga aatcattacc
      721 catttagctt tttatgtagg atggccaaaa gcttggtcag cttttagttt agcaaaagaa
      781 atctatttag aagaaaatag cgaagataag aaagaggcgt aaatgacgac aaaacacgaa
      841 gaaatacaca atggcgtgat ttttccagca ggtgaaaaaa acgaagctta cgctcaagta
      901 tttaccgggt aaagctatct gaatatgtta gtgaatgatc ccgaagtttc tgttggtgta
      961 ggtaatgtaa cttttgaacc aggttgtcgc aatcactggc atgtccataa agacggctat
     1021 caaatcttac ttgtgaccgg aggagaaggc tggtatcagg aagaaggaaa gccagcaaga
     1081 cgattaatgg ctggagatgt cgtcgtaacg cacaaaggga ttaaacattg gcatggtgcg
     1141 acaaaagaca gttggtttga acatttggcg atcactgctg gtaattcaga gtttttagaa
     1201 cctgtaacag atgaagtgta tgaaggattg gaaaatcaat aatgtcaagc ttgaacagct
     1261 cctgaaagta gattttaatc gaactttcag gagctgcttt tttgattcag tcttttataa
     1321 atagaaaata gggttaataa taggaatgag aagcacgtcg aaagatctta atattgataa
     1381 tcggctgggc atacatgatt tccacgtcat caatcaaact acctaacaga tgataatctt
     1441 cccgatcatg tcggggatta cctaatagtt cttgataata gatttccata ctagggtccc
     1501 gaggattgtt cagcaaatca gcaagtacat ctaaatcttc tggctgagtt tctgccgtac
     1561 cctgtatctc tatgaaaagc ccttcctcag caaattctac tccaatacgt atttcagtta
     1621 tttcatggga atataaataa gtaagaagct catcgatgat tctttttgat aattcttttt
     1681 ccaagtgtct ttttttcctt tctgacagcg gtaaagttat ctagtatcgg tacaaggata
     1741 cctgcaacga aaccagttga aaaaccactg ttatatagat ttagtcctcc gtgcaaagtg
     1801 ctgacgttat ggacaagagc aatatgtagt acaccagcta ttacaccgta aaaactgccg
     1861 taaaaaccgc ttatgggcgc gagacctgtc ccaaacacag ctgcaacaag catgccagta
     1921 ctggtgatct catgaaaagt tccaaacaaa gaagcaataa atattccgac tagtatcgga
     1981 aaactgtttt ttaaatgaca accaaaagca ctgaaaccca ccgcagataa aatagcaccg
     2041 atgacaggtc catttaactg accgccaacc agcaaaacat aagaagacag catcagacca
     2101 actaagccta tgttgatcaa agtagcacca ataccagaat tagcaatgaa atcagttgtc
     2161 aattttccag atgaatcgaa aatttctcga atccccaaaa gccgaaagga attataatag
     2221 aaaccaattc cgactgtcag cgaaaaaaag ccaaaaataa tccaaatgag aggaaagtga
     2281 tattcattga aaaccaatgt attttcgacg attgaatagc tgaataacct caaaacagca
     2341 gtaaaagtca tcgcaatcaa cccagaggtg aatccaatat tataaagtgt gaatccacga
     2401 tggaaaacta aagtttgagc agctaagggc ggaagaagta gaccaattgc tatgccagct
     2461 aaattcccca ataaaatacc tatcagtaaa ggaaaacgca taccaaaagt gatataacta
     2521 acgaccggtg ataatgcgct accaaacagt ccgatcatga tgtactgaga aaaaggttta
     2581 ttgacaaata cagcgtatag atacacacct aaaatgattg agatagaatt ataaaggttt
     2641 ttgccaaaaa aagagaaacc ggtaactgtt agaatcgaag caaataaggg accgtttaaa
     2701 atcaccttgt gccgataagc taaaagcaca gaaagtaagg tcaaaatccc tacatttatg
     2761 aatgcgcttc cgaaaccgcc taaagcaaaa taatctgtca aaagattgct tggcgaggta
     2821 agaatagtta gcaatccact ccaattatca gtaggagtat tagaaagcaa gccaataaaa
     2881 agaaaaaaaa cacctgtaaa accaagataa aaatactttc ttttctgtga aacgattcgt
     2941 ttttgaggat gaatataaat aacgtcagtt tcgttttcca ttacacacct tctttttcat
     3001 tttctttaac tataacttga aatgaaagat ttggacaact attatttagg tcaaaactaa
     3061 aaatgtaagg gaacccattg aatttaacca aattttttta attttgcaaa aaaaagttaa
     3121 agaagactga aaaaaaattt aaaatataac gttttgagta aaatgtttgc aaaaaatgct
     3181 aaaaataggg tacaataaaa caacatatac tgcagtaaag aagtggggga gttttaatga
     3241 gaagaaaagt ctataccaag gatcaaattc taaaagctgc ttatgatgtt gttgcaaagg
     3301 aagggtttaa aggatttact gcgagaaata ttgctaaaaa gatggggatt tcgacacagc
     3361 cgatttatct ggaattcaaa aacatggaag atttaaaaaa tacgttgctg gattctatct
     3421 ttgaacaact aaaagaagat gttttcccta ttgaacgtac tggtgataaa atcattgatc
     3481 tcggtttgaa ctatatctac tttgcaaaaa agcatcatag tctttacatg gcattgtaca
     3541 ttgatgaaca tggggacgga aagaaaatgc atgatttttc atacaattat ttccgtgaaa
     3601 tagttaaaga agatgaaaga tacaatcaat tatcggatga atatgtcgat gctctgcata
     3661 ctggtacatg ggtagcagtg actggcttag cgtcattgat gtcatcaaac atcatgcatc
     3721 caacagaaga acaaatcatt gcactgatca aacggacaat tgactcgatt ttagcattag
     3781 ataatcctga aataatcaga tagtgttcta tcagtatgaa tttgatcggt aagtccgttg
     3841 gctgcaaact gtcaatggac ttttttgcta aaaaaagcga tttctgttat ttatctatta
     3901 gaaataggct tgacatttcg tgaaaaaagt ttaaaaatag tacttacgga aggttggcag
     3961 agtggtaatg caccggactc gaaatccggc gaaccggcta ataccggcgc gcaggttcaa
     4021 atcctgtacc ttccttatat caacgattag aggttgtgaa gaagctgtcc tgcatcaagt
     4081 aataagaacg gccaaacaaa aatagctttt cctatttttt cgtttgattg ggcttattac
     4141 cgcagatgca agcttttgaa caccgtttgt tcggaataag gggctgtgac aagacttttg
     4201 tcacagcccc ttattgttgt acttttaacc tacaatattt tttgattccc cctgagtact
     4261 attttttata aatgctagta aatcaatctt ctttggcctt tttttgataa aaaaaatact
     4321 agatgtaaga taaaagtgta aaaacgattt gttttaggac gggaaacgat gtgtaccgca
     4381 atcacttatg caacgaaaga ccattatttt ggaaggaatt ttgattatga gatgtcttat
     4441 aatgaagtgg tcactattac tccaagaaat tatcgtttcg attttagaaa ggtaaaaaat
     4501 ttagataaac attatgcaat gataggaata gcagctgggg tatccaacta tccactctat
     4561 tatgaggcta caaatgaaaa agggttgagt atggcaggac tgaattttcc tggcaatgct
     4621 gattataaag aattacaaga aggaaaagat aacgttgcac catttgagtt tattccatgg
     4681 atactagggc aatgctcaac catagacgaa gctaaagaat tattggctac tatcaatcta
     4741 gtcaatatcg attttagtga aaaactaccc ttgtcacctt tacactggtt attagctgat
     4801 aaagaaaaat cgatagtaat tgaaagtatg aaagatggac ttcatttata tgataatcct
     4861 gtcggtgtgt tgacaaataa tccgccattt gattatcaat tatttaatct gaacaattat
     4921 cgctcgctat caaatggaac accagaaaat catttttcaa accagatcag cttgaatgtt
     4981 tatagtcgtg ggatgggagg attgggctta cctggagatc tctcttctgt ttctcgattt
     5041 gtaaaagcca catttactaa aatgaatgct gcatcaggtg attcagaatc tgaaagtatc
     5101 agtcaatttt ttcatatact aggctcagtc gagcaacaaa aaggagtatg cgatactgga
     5161 gaaagaaaat acgaatatac gatttattct tcttgctgta acgttgataa agggatttat
     5221 tattatcgta cgtatgaaga cagtcaaatt actgctgtaa atatgaataa agaagacttg
     5281 gatcgtcatg aattggtcag ttatccagtc ataaaagagc aacagataaa ctatgtcaat
     5341 taattaatga atagatccgt ttggtatcat attttctgat acagtcataa ttagacacga
     5401 tctttaggtc gtgtcttttt ttgctggttt ttttggaaat agcatccact aatatttttt
     5461 taacacgaag agaataaggt agcctcaaaa aagaaaacat ggtaaattag atattgggac
     5521 tttattttct gtaaaaacag aaactttcat cttttgtttt cacgtgacga aattgtaaga
     5581 gagaaaatat tcgtccgcta tttattctat gataagatgc atatgaatga aataaacagt
     5641 aattttatat taggatgtga aagttaaatg tgcggtattg taggttttgt taacgacaag
     5701 gacaacaaaa aaacaattat taatacaatg atggatcgaa tcgttcaccg tggacctaat
     5761 agttcaggag aatatatcga taaacatgtt gctttaggat tcagaagatt aagtattatt
     5821 gacttagagg gcggtactca gcctatttac aatgaagatc ggacaaagat cattattttt
     5881 aatggcgaaa tctataatta ccagcctttg agagaagaat tgattgcagc tggccacgtt
     5941 tttcaaacac atgcagatac agaagtcctt ttgcatggtt acgaagaatg gggaacagaa
     6001 ttattgcaaa agatccgcgg aatgtttgct tttgctattt gggataatga aaagaatgag
     6061 ttattcggtg caagagacca ctttgggatc aaaccatatt attatgcaga aatgaacgga
     6121 acatttatgt ttggttcaga aatcaaaagt tttctgcctc atcctgattt taacaaagaa
     6181 ttgaacaaag aagcgttgaa accttatatg actttccaat attctccatt aaacggcgaa
     6241 acattcttca aagatgtcta tcgtttgcct gaaggccatt attatacgta taaagatgga
     6301 aaaatagaca ttcagcaata ttgggatgca gattttgaaa caaaagaaac gcattcccga
     6361 caagaatgga tcgaaaaaat cgacgaaacc gttcaagctt ctattgaagc acatacggtc
     6421 agtgatgtag aagttggttc tttcttatcc agcggggttg attccagtta tgtaacctct
     6481 gtattgaaac cagatcattc tttctcaatc gggtttgatg ataaaaccta taatgaagcg
     6541 attgaagcca gaaaactaac cgaactattg gacttagata atacagcagc agtgatcgac
     6601 ggagacatgt cgttcaaagc attccctttg atccagtatc acttagatga acctgattca
     6661 aatccttctt gcgtaccttt gtacttttta gcaaatcttg cttcacaaag tgtgcgggta
     6721 gtccaatctg gagaaggggc ggatgaactg tttgcgggat accaaacgta tggattccat
     6781 acgaattcaa aattcatccg tgtgattgct caaggattga aaaaattacc aaaaggaaca
     6841 cgctataatt taggacgtaa aatcggaaaa atgaaaaatt tccatggacg gatccattta
     6901 tatgaatcac ttgctcctgc aaaagaatat tttatcggtc atgctcgagt gttcgaagaa
     6961 tcagaagcat cagaagtgtt aacaccaaaa tatcaaacag caccatctgt ggatgaaatc
     7021 atgactgttc attacgagaa aacagaagga atcaaagatg aagtcaataa gatgcaatat
     7081 gtcgatcttc atcaatggat gccaaaagat atcttgttaa aggcagataa actttctatg
     7141 gcaagttctt tggaagtccg agtaccttta ttggatatcg aagtcatgaa attggctcaa
     7201 caaataccaa gtaagttttt attgaatcaa aacaacacaa aagatatttt cagacaagct
     7261 gcgaacaaac atcttccgga agaatggtcg aatcgtgtga aattaggatt ccctgtgcca
     7321 atcaaagctt ggttgaaaga agaacacggc tacgaacaag tcaaagcatt attcgaagca
     7381 gattttgcaa aagaattttt tgatcaagaa aaaatcatga agttgctaga tgaccatcac
     7441 gaaggacgta aagaagagca aagaaaaatc tggacaatct tcagtttctt gacatggtat
     7501 aaagtctact ttgtagatga aacgattccg caagcagaag caatcgacta tgtgactgtt
     7561 tgatgaaaat caaatatcaa taaaaaagag gttttctcct accgtttaac actgagtgat
     7621 aagaaacaat ttcaaaaaac agctcttccc gttttataga tattatgact tatggttcgg
     7681 agttggcaga ggaacaccat tgtttcgaac ataagggggt tgtgacacga ttttcgtcac
     7741 aaccccttta catatgttca ataatcgatt tctttttctt ttgtcccatc ttttttataa
     7801 atgaacaaag ttgattcttt gttttgtgct acttgctttc ctttttcgat tgcttctttt
     7861 tttgtatcaa aatggttgct tgcctttttt gcccctttag atttcacgat ccattgttct
     7921 tcctcatatt ctacgatcac atctgaatca agcagttttc cagcacgagg attcgtatcg
     7981 tgttcatccg tcttgcttgg atttttttct ttttcgaaag ttcgtttttc tgattcacta
     8041 gcatcttcat accatttttt tgcttgatca atggcaattg ggatcgcacg gttatcaggg
     8101 tacccttcat ctaataatgc atttgcgatg tcgattgctt tttttcttgt caatttatct
     8161 aagtttttca ttgatgcagg aaagtccttc atgttccatg gcatattagt atcccccttt
     8221 attaaaaaac ataatctata taaacaagtg taagggtatt tatacaaaaa gaaaaagatt
     8281 ttgtttgaga agtaaaaccg ttgtatttat cgtcaagtag ttttacttaa caaaatcgtg
     8341 aaaaacacta gcaatctaga aaaaatcatg gtaagataat caagtctgag agtcatctta
     8401 gaggattctt gtaaaaatgg gagggaaaaa catgcgcgta aacatcactt tagagtgtac
     8461 ttcttgtaaa gaacgtaact atctaacaag caaaaacaaa cgtaacaatc ctgatcgttt
     8521 ggaaaaacaa aaatattgcc cacgcgaacg caaagttact ttgcaccgtg aaacaaaata
     8581 atgtttttaa agttctcaat tccttgctgg tcaaagaatt gagacttttt ttatctcaaa
     8641 acatctaaaa tcataaggag cggataatat gtttatttcc atgtgggcac aagataaaaa
     8701 tggtctgatc ggcaaaaatg gtttgttacc atggcgattg ccaaatgata tgcgtttctt
     8761 tcgcgaacac acaatggatc gaattttagt gatgggaaga aagacctatg aaggaatggg
     8821 ggatctttca cttccttatc gccatatcat cgtgttgaca acgcaaccaa attttcgcac
     8881 gaaagaaaat gcggaagtaa tgcattcgat cgatgaactt ttgtcgtata gtcaaacgat
     8941 ctcagaagat atctatgttt cgggtggtag tcggattttt caagaacttc ttccttacac
     9001 tggtcggatc tggcgaacat tgatcgatag cacgtttgaa ggcgatacgt atattgggaa
     9061 tattgatttt tcagacttcg cccttataga ggagtatgag gggataacgg atcaagaaaa
     9121 tctatatgcc catcgttttc aaaaatggga aagagtaaaa aatagggttg cttcaaaaga
     9181 agaataaaat ctatgcgtct agtttgttaa taagaaaaaa agcatctcac gactaatcgt
     9241 gaagatgctt tttttagtta aaatcaattt agtttttctt tgatatcatt gaccatttcc
     9301 tgactggcaa taggtccgtt atatagccaa ctagattcag gtccgaattg cagtacatga
     9361 ttgtctgtca ctgcttttag attcttccag cttggttcat tgaacatggc agcatctttg
     9421 tcactgttca caaggatgat gtagtcagca tctaattcag ctagcttttc agaggaaaca
     9481 gcagaccagt cagaagtggc ttttttggag acctcttcca ctagatcagg aacaccaaat
     9541 tccagatcgt cgtatacgat tcttccactt gaccgatttt tgctgaccat aaaggctgag
     9601 ttgttggtca cccacaacac tgcagctgtc ttgttctcga ttttatctgc tagttcttct
     9661 cgagttttct tgatggattt ttgataatcg gaaatgattt tttctgcctc tttttctttt
     9721 gccaaagctt tgccgacatc ttttagttgt tcttcccatg tgacatctgt tccgtttttt
     9781 acaacataag ttggcgcgat tttgctatat tgttcgtatt tcccgccttc gacagtagcg
     9841 gaagaagaaa tcaacagcaa atcaggctca aagcttaaca ctttctcata aggaaggtca
     9901 taagaaatcg ttgggacatc ctttaaatca tcttgcagat aatcttgaat ggaaccactg
     9961 ccaacagtcc actgggcgat aggtttttcc cctagtgcaa tcagatagtc ttctaagtaa
    10021 ctgccgatga tccgtttggg cgcgtttgga atctctactt tatgtccaag tgtatctgtg
    10081 actgtatgag tactattgga tgaagtcgtc gaattggcag ttgtagctgt ttctttatct
    10141 gaagaggggg agcaagcgct cagtactcca gccagcagca atatgctaga taatgtgaat
    10201 acttttgttt ttaatttcat ttctttcctc ctaacataat tgagaatctt tctcattggt
    10261 tattattcct gatatttcga acgctgtcaa ccgcttcatt tggctataga tatgaaagta
    10321 gcgcttgacg aaatgttgaa acagcgtcat aattgagaat gattttcagc gaaaggatgg
    10381 gtgaaatgac gagattaaga gcagctgaca ttgcagccgg ctacgaaaac aaaaaaatca
    10441 ttgaaaacat gtctgttacg attcctgaag ggaaaatcac tagtttgatt gggccaaatg
    10501 gaagtggaaa atctacttta ttgaaaagtt ttacccgtat actacctgtg aaatcaggaa
    10561 agatcattgt caataactct gatatctcta catacagttc aaaagaactt gcaaaaaaaa
    10621 ttgcattatt agcacaaact agtgaacacc ctacaggtat gacagtagaa gaagtagtat
    10681 cttacggacg atacccttat caaaaactgt tcagtggcat ggacacgcaa gatcatgaag
    10741 caattgagtg ggcgttggat gctacaggtc tagctggatt tcgtatgcgg gatatcgcaa
    10801 cgctgtctgg tggacaaacc cagcgtgtct ggatcgctat ggcgcttgct caggaaacag
    10861 atatcttgat cttggatgaa ccgacaacat ttttagatcc ggcacaccaa ttagagatcc
    10921 tacagttatt gactgaaatc aatcaaatga aacaaacaac gatcttgatg tcgattcatg
    10981 acattaatca tgcttcacgt ttttcagatt atttattagc aatgaaaaac ggtaagttga
    11041 tcacagaagg tacacctgaa gaagtgatca caaaagagtg tatgaaaaaa atatttgaga
    11101 ttgatgcaac gattgtcacg cttccaagaa caaataaacc agtttttcta acctatgatt
    11161 tgttgccaaa agagcaggag gatcaattat gaggactatg aagaaaaata gactcctgct
    11221 tttggtctgt tttgttcttt tgattatttt ttttgttgct ctttcaaatg gcgcagcgac
    11281 tgtgggaaaa gcagattttt ggcaggcgtt gtttgatttt gatagatcaa aacaaagtca
    11341 ccaaatcata cgaaatattc gtttgcctcg tgtggcggga gcatttcttg tagggagttg
    11401 ttttgcattg agcggtgcat tgatgcaagg agtgacaaga aatcctttag cagattcagg
    11461 cttactagga atcaacgcgg gtgcttcgtt ggcaatggcc cttagttttg ctttttggcc
    11521 taaagcttct gatttttctg tgtttctaat gtcattggca ggttcttttg ctgtaacttt
    11581 gctcgtattt ggttttttgc agttttcgaa aatgggcatg aatcctttac agctgatttt
    11641 agctggcgta gctattagtt ctttttttac tgctatcagc caagcactta cccaattctt
    11701 taatctgcag caggatcttg ctttttggtt tgtaggcgga gctgccaata tcacatggaa
    11761 acaattagca gtattgtttc ctttatatat acttgctgtt ttggtttctt tttcattagg
    11821 aaaagcaata tctttgattc atttgagtga aacacatgca ttagctcttg gaagtcatcc
    11881 taaacgaacg cggttgattg ttttctgtct agttgcagtg ttagcagcca ttgccgtttc
    11941 gttagtcgga cctgtttcat ttattgggct gttggttccg ccagttgttc gcggaatggt
    12001 tggaacggac tatcgttatg ttctacctgt ctctttttta gttggcggtg gattggttat
    12061 gttggcggat tttacagcga ggatgatcaa tccccctttt gaaacacctt ttggattagt
    12121 cattgcattg attggcgttc cgtttttact gatacaagta aggagggaac aacggtgaaa
    12181 aaaatatatt ggacgttcta tttcttgctt tttgtacttg tcatcctgtc tataggtagt
    12241 ttaatgattg gtacgccttt tatttacctg gatcagcttt ttgccacgtt catagggaaa
    12301 gaatcatctt ctttggcgct gattgttaga gaatttcgag ttcctcgatt actgatcagt
    12361 ttattagcag gagcttgtct tggcgtttct ggttatatct tgcaaggagt aacaagaaat
    12421 gaattggcag attctagtat attgggcatt aatgccggag caggattttt agtgatgctt
    12481 tatttaggtt tcttttcgca ctattcgctg ccgtttctat tgctagttgt agcctttcta
    12541 ggtggtgttt tagctgcttg ctgtgtctat cttgcggcct atgatcgaaa tggctttttg
    12601 ggtatgaatc gaatcttatt gtccggtatt gccgttaatg ccggcctttc tgcattaaca
    12661 ttgctatgca cgatccaatt atctaaggaa aattatggat ttgtgaatgc atggttagcg
    12721 ggatcaatct ggggtgcgag ctggccttat gtttgggcat tgcttccatg ggcggttatc
    12781 ttaattccgc taggaggact ttatgcacag aggattgtat tgctatcatt tggtcaagaa
    12841 cgcgcgcaag gattaggcct taacgtggct aaaagtcaaa aaatcctttt gttgttagct
    12901 gttgctttgg caagcggtag tgtagcggtg acaggtagtt tatcttttgt aggactacta
    12961 gctcctcatg ctgcgaagct agtcgtacgt aaagaaaata actggacctt tgttctgagc
    13021 ggtttgttcg gttccatttt tctgcttagt gcagatatgg ttgctagagt gattctacta
    13081 agtggggaag tacctacagg aattttgatt gccctttttg gtgcgccata ttttctgtat
    13141 gcacttttta tcagacaaaa acacgcgctg aacagttgaa attcattaaa aaaggaaggc
    13201 caatcatttc taggtcttct ttttattcta tttatgcatt tatttgaaag ttcgattgct
    13261 atttttcttt ttttcctata ccattttatg agagaaggaa gggtgagttt ttggaccaat
    13321 tgaaagtaag attacgccgg aaaggaatcg aaaagctgaa aaatctggcc aaaagtggca
    13381 agaaaaagga aaaagaacag cgaatctata aacagctatt cgccagcgag tactggaagt
    13441 cagcaaaagt gatagggacg actttatcga atgaatttga actggataca cagccggtta
    13501 tccaacgagc agaaaaagaa caaaagaaaa tagttgtacc taaaactttg ccgaaacgtc
    13561 agatggcgtt ttatgaaatg gatgaacagt ccgttcttgt acgttcgagt tttggtgttc
    13621 ttgagccaca atcagagaag ctctatttgc cggagatgat cgatctcctc attgtaccag
    13681 gtattatcta caatagagcc ggttatcgaa tcggttttgg cggtggttat tacgaccggt
    13741 atttaaataa ttttcataaa aaaacatgta gccttgtttt tcgtgaacaa ttgaatgaac
    13801 aatggcaacc ggagagcttt gatcagaaga tccaacaatt gtttattgac taaaatgaaa
    13861 tgggaggttt tcatgaatta tcaacagcaa ataaaattgc gtgcatggtt aagacgaccg
    13921 ttatggacct atgctttttt agggatacag acagcggtct tcctcatgat ggaattattt
    13981 ccgcgacttg agatccctta ttatgccgga atgtatggtc cttatcttgt tcacttcaat
    14041 gaatggtggc gtttagtgac accaatcttc atccattttg gactgatgca tttcgtaatg
    14101 aactcactga tactgtattt tatggggcag caaatcgagg caatctatgg ccattggcgt
    14161 tttttcttga tttatatgtt cagcgggata atgggaaata cagcaagctt tgcttttaac
    14221 gaagcgaatg ttctgtctgg tggtgcaagt acttcgatat tcggtttgtt cggtgcgttg
    14281 tttatcttag ggtttcattt taaacataac ccagcgatcc agcagctagt cagacatttt
    14341 cttttattta ttgtgttgac atttgttttt ggtcttctgg atacctctgt agatgtttgg
    14401 ggacatgtcg gtgggctaat tggcggatta gtgttaggga atgttttggg tctaccgaaa
    14461 caacagacaa gctattcgat acaccaaagg attctttcta cactagtttt tgtatttctt
    14521 tttgtaatat gtattttact gggtttaaaa aaatatggtt tacttgtata atatgaagga
    14581 agtgcttaaa gatggagagc ttatatgatg tacagcagct gttcaaaaaa tttggcatct
    14641 ttatctatgt gggtgcacgc atctatgaca ttgaattaat gactatcgaa ttgcgtaatc
    14701 tgtatgatag ccgtttgatt gatcatgata catacatgac agcgtggcgc atattaaaaa
    14761 gagagcaccg aattgaagaa agccgaaaga aaggaaattt gtaatggaca agaagattat
    14821 tgggattgat ttaggtggaa caacagcaaa atttgcaatt ttaacaccag aaggagagat
    14881 tcagcaaaaa tggagcatcg ataccaatat tttggatgat ggtaagcata tcgttcctga
    14941 aatcattgaa tcaatcaatc atcgtttgaa tttatataat atgaaagccg aagactttat
    15001 cggaatcggc atgggtaccc caggaagcgt ggacagcgaa gctggaacgg ttattggtgc
    15061 gtataactta aactggacag aagttcaatt tgtgaaaaaa ctgatcgagg caggaacagg
    15121 aatcaaattc gctatcgata atgacgcgaa tgttgctgct ttaggtgaac gatggaaagg
    15181 tgctggagaa aacgatccag acgttgtatt cgttactcta ggaacaggtg ttggcggagg
    15241 tatcgttgct ggcggaaatt tgattcacgg cgtgagcgga gctggaggag aaatcggaca
    15301 tatcactgtg gatcctgaag ggttcgaatg tacttgcggt aaacgcggat gtttagaaac
    15361 tgtatctagc gctacaggtg tggtacgcct aggtcgtttc ttggcagaag aatacgcagg
    15421 ggattcaaaa ctgaaagcaa tgctagacaa cggagaagaa gtaacaagta aagatatctt
    15481 tgaaatggct caagaagacg atccgtttgc attgatggtt gttgatcgcg tatgcttcta
    15541 cttaggttta gcttgcggaa acttggggaa tacattaaat ccgtcaagta tcgtgttagg
    15601 tggaggagta tcggcagcag gagaattttt acgtagccgt gtagagaaat attttaaaga
    15661 atttactttc ccgcaagtaa gagaatcaac aaaaatcaaa ttagcagagc ttggtaatga
    15721 agctggtgtc attggtgctg cctcattagc cttgaaattt gcttaaaaaa acggaggcat
    15781 tacatggttt tatgggtaat taatatcatt ttgctattga ttgtgcttgc aatcatcctt
    15841 tgggaagtct atctacgagt aatggccaaa cgttcagcaa cgactttgac agaagaagaa
    15901 tttcgagaag gaatgagaaa agcacaagta atcgatgttc gcgaaaaaga tgtgtttgat
    15961 gctgggcata ttttaggtgc aagaaacatc ccttacactg ttttgaaaga ttcattagga
    16021 tctattcgaa aagaccagcc tgtctatatt tacgatcaaa agaaaagttt gagcatccgt
    16081 acagcaaata aattacgtaa agcaggttat caagagattt atttacttaa gggtggatat
    16141 gacggatggt ctggaaaaat caaaagtaaa aaagtataaa acagatgatt gtatgacagc
    16201 tttaaaaacc gaatcaatca ttagattttg accaagatca catgaacggc gcaatggata
    16261 gttgtgccgt tcattttttt tattttgtgc tattatctac atatataagc taagcgaaaa
    16321 ggtagaaaga aggaaacaac tatgatcaaa ttaaaacgtg tttaccttga tgctgaaaaa
    16381 gaggatggct atagaatact cgtggataga ttctggccac gaggaatttc aaaagaaaaa
    16441 gcgcagttag atctgtggtt gaaagaaatt gcacctagta atgaagcacg caaaactttt
    16501 gaccatgtac cagaacgatt tgcttcattt gcaaagaaat accagcaaga attgattttc
    16561 gaagaagaaa agaagcaagc tatttttaaa ctcagtcaac ttatgaaaga acatccagtc
    16621 attacttttg tttatggtgc aaaaaatgaa catcagaacc atgctgtttt attgaaaaaa
    16681 tttatcgaaa tggaggcatg caaatgaaaa tagggatcat tggtgccgga cctagaggat
    16741 taagtgtagc agaaaggatt ctccataata atcaggacag acattctctt gaactttttc
    16801 ttttcgatcc caatggtcct ggaggtcgag tttggcggtt gaatcagcca ccggagttat
    16861 taatgaattc tgtttctcaa caagtgacgc tttttacaga tgaaacattg tccagtggag
    16921 gattggtaac acagggaccc aacttgtttc aatggagtaa aggacctgct acagattata
    16981 tcgaacagac ggtaaccaaa aataagcaac taattataga agaaatcaat cgtctggaaa
    17041 aagatcagca atctactcga tgtttatacg gactctatca acgctggttt ttctcaaagt
    17101 tgcaagaaga atttccagct tctattcagt ttgtttattc tttagtaaaa aaagtaagca
    17161 aaagaaaaga tggttatctt ttacagacag atcagcaaag ctattctatc gatcagcttg
    17221 ttttagcaag cggtcactgg gaaaatgatg agacagaaga agaaagagtg tgggtagact
    17281 acgcaaagaa aaatgtttta ctgtatcagc ttccttctaa tcctgcagat gtagatcttt
    17341 cgaagatacc gcctaaagaa aatgtctttt taagaggtct aggcttaagt ttttttgatt
    17401 atgtcggatt gtttacagta tcaagaggag ggcgcttcga gaacgaaaag ggaaaactgg
    17461 tctatcatcc atcaggcaag gaaccgattg tatacagtgg atcaagaagg gggctccctt
    17521 attaccctcg aggaagaaat caaaagcaag gcggagcaat ggcatggcca cggttactaa
    17581 caaaagaaaa cctggagcaa tggcatcagt cgggattttt gactggcgaa acattttttg
    17641 actatctgaa aaaagacgtt gagttgtttt attacaagaa aatcatcgaa gaatatcact
    17701 tgccgattcc aaagaaaaat tttgagcatg attttctctc cctctcttca gaggagtgtc
    17761 tcggaaagca tccagcacta atcccttata gatgggattg gtcgtattta gaaactcctc
    17821 ttgcctatca gaatgagccc ttttcagaag ctagccgaat atttattgaa gggcagataa
    17881 aagaagcgga aaaaggcaat tgcacaggag catttgcttc aacatttgat gcattaaaag
    17941 actggcgtga tccaatccgg caggctattg aatggcacgt tttttctgct aaggaataca
    18001 aagaaatact atggggatgg tttactcatc taaatagctt tttaacgatc ggtccgccaa
    18061 tcattcggac gaaagaatta gcagcactga tagatgcggg tatttttcat ttgatcgaac
    18121 cgccatttga tattcagcaa aagcacaacc gctatgttat ttctctacaa gataaagaaa
    18181 tcagcagtcg ctatttgatt gaagctcgat tgccacaaac aagcttttct cgttcaagta
    18241 atcctgtcat aaaaaatttg aaggaaaaca aacttgttcg ccctttttca ttttcagata
    18301 atacaggaag ttatcattca gaagctatag atgttgagcg aggtacgaat cgcgtgattg
    18361 atgcttcagg ggaaatccag tctgactttt attgctatgg aatacctgtt gaaggtgtgg
    18421 attggctaac tgcagcagta gctagacctt atacagatcc atggaatctg agacaggcag
    18481 ataaaatagc acaacttata ttgactgaca aataaaaata cgcatgatga cttatctgtt
    18541 ttttagaaag tcttcatgcg tatttttaaa taattattaa cgagaaacgc gtttacgcat
    18601 tgctttttta cggttttctt cgatcattgc ttctttttcg tcaattggat cgatcactgt
    18661 ttttttcaca gaagcaacca aaccagcaac ggcagcggct gttgccagac ttcctactaa
    18721 tattccagat acaaattttt tcattgaatc cacctcattt attatttgtt atatctatta
    18781 tggaacaaaa gagaaaaaaa ggctagtttt acgtttgttt gtaaaccttt gcaaaaaatc
    18841 cttgaaaatg tttactaaat gttttattat aaatttacca tatgaattta cgaaatttta
    18901 ctttgtttgt tttcaccata aaagtaaact tcgtttttta aattatatag aatcaacaaa
    18961 cacaatttta ctaatcaaag gagtgttttt tatgacaatt ttagtactag gcggagccgg
    19021 ttatatcggt tcgcacgctg tagaccagct agttcaaaag gggtatcaag tagctgtggt
    19081 agataatttg ctcacaggtc acaaacaagc agtacatcca gatgctcatt tttatgaagg
    19141 agacatccgg gataaagaat ttctacgttc cgtatttgaa aaggaaccga ttgaaggagt
    19201 aatccatttt gctgcaagct ctttagtagg agaatctgtc gaaaaaccat tgatgtattt
    19261 caacaacaac gtatacggta tgcaaatctt acttgaagtc atgcacgagt tcaacgtgaa
    19321 taaaatcgta ttctcttcta ctgcagcaac ttatggagag ccgaaagagt ctccaatcac
    19381 agaagataca ccagctaatc ctaaaaatcc gtatggtgaa agtaaattga tgatggaaaa
    19441 aatgatgaag tggtgtgacc aagcatatgg catgcgctat gtcgctttgc gctacttcaa
    19501 tgtagccgga gcaaaagcag atgcatctat cggagaagat catacaccag aaacgcatct
    19561 tgtcccaatc attttacaag tagctcttgg tcaaagaaaa gccttggctg tttatggaga
    19621 cgattatgat acaccagatg gaacatgtat cagagattat gttcaagtag aagatttgat
    19681 tgctgcacat attttagctt tggaatactt gaaagaagga aatgaaagca acttcttcaa
    19741 tttaggcagc agcaaaggat actcagtcaa ggaaatgcta gaagcagcta gagaagtgac
    19801 aggaaaagaa attccagcag aaatagcacc aagaagagct ggagatccta gccgattagt
    19861 cgcttcaagt gaaaaagcga gagagattct gggctggaaa cctgaataca cagatatcaa
    19921 agcgattatc aaaacagcat gggattggca tgtcagccat ccaaatggtt atgaagaata
    19981 ggggaatgag atatgtcaat cagtcaaacg atttgtgatt ttgccacact tgcgatcgaa
    20041 gctggcggat ggatggaaat ggaccgctta tatttacaga atcgtctttt agggatggta
    20101 ggagaagatg ctctagaaag cattgaagta agaccagtta aggaagaatc tgtagactta
    20161 ttggatcagc ttgtcgctaa ggcaagagaa aatggtgttc tttcagaatt gccagcagaa
    20221 caagagattt ttgaagcgca attaatggat tttctcacac cgccaccttc agtcgtcaat
    20281 gcattctttg cacagcacta ttcgaaaagc ccagaggaag caactgagta tttctttaat
    20341 ttaagtaaga aaaatgacta catcaaaaca agagccatcg caaaaaacat caaatttccg
    20401 gcagagtcgg agtatggtga actggaaatc acaatcaatt tatctaagcc agaaaaagat
    20461 ccaaaacaaa tcgcagcaga acgcgaggcg gtaaaagtag actatcctac ctgcatgctt
    20521 tgcatggaaa acgaaggata tagaggtcgc ttgaactatc cagcccgcac caatcaccgg
    20581 attattcgaa tgaatctaga cggtgaaagc tggggattcc aatattcccc ttatgcttat
    20641 tacaatgaac attcgatcat tttatcagaa caacaccgcc cgatgaaaat cacaaaggaa
    20701 acatttgcta gactgcttcg gattactgaa gtcttgcctc attactttgt tggttcgaat
    20761 gctgacttac caattgttgg aggatcgatt ctttctcatg atcattatca ggcaggacgt
    20821 catgaatttc ctatggcaaa agcaccgata gaaaaactta tcaaactaga agattatccg
    20881 caagtcatcg caggagtcgt gaaatggccg atgtctgtga tccgtttgca atcaacagag
    20941 aaaaacttgc ttattgaagc agctaactca gtattggaaa aatggaaagc atattcggat
    21001 gaaacagtat ctatcagagc tttttcagca gatggtacac cacaccatac gattacgcca
    21061 attgcacgaa gaagggacaa ttactttgag ttggaccttg tccttcgaga caacaatgtt
    21121 tcagaagaac atcctgatgg gatttttcat ccacataaag atgtccaaca tatcaaaaag
    21181 gagaacattg ggttgatcga agtaatgggg cttgctgttt taccacctcg tttgaaacca
    21241 gaactagtag aagtagagca tttcttatta ggagaagaaa accagatggc tgaatatcat
    21301 cgtccgtggg cagaacaatt aaaaaaagaa catcctgatt tgacgaaaga tacggtcacc
    21361 gatgtcgtac aaaaatctgt aggtcaggtt tttgcacgtg tactggaaga cgctggagta
    21421 ttcaagcgag atgaaaaagg acaggcagcg tttgaacgat ttactgcgat tttataaacg
    21481 taaaaaagga ggagagacaa atggcaacaa ttaaagacat tgcgcagttg gcaggagttt
    21541 ctcctgctac tgtttcaaga gttctcaact atgatcctga tctttccgtt ggcttagcca
    21601 caaagcaaaa aatttttgaa gcagcagaac gcttaaatta tacgaaacat cgtaaaaatg
    21661 cagcaaatac taagcaaacg cttcgcctga tccagtggta cgacgatacg gaagagcttg
    21721 aagatcttta ttatttatct attcgcctag gaatcgaaaa aaaggcagaa gaagcaaacg
    21781 ttactttatt gaaagaaacg tgggattcca ttagtgatct tccggcagat ggaacgatcg
    21841 cactaggaaa attcgacgaa gctcagttat tagaaatgaa aggatcccaa aatgccttgc
    21901 ttttggtaga ttctgacggc acttctcaag gaatcgactc tctagttgtc gactttaagt
    21961 caagtgttca aaaagtcatt gaacactttg tctcccataa agcgcaacat atcgctatgc
    22021 ttgctggaac agaatatacg aagaaaaacc atcagcggct aaaagatccc agaattatgg
    22081 catttcaaca agttttggca gaaaaaggct acgacgaagg tccaattatc gaagcagatt
    22141 tttctgtcga gtcaggatat caagcagtga agcagtattt aaaaacgaat ccgcatgttc
    22201 ctgatgcgct ttttgcagct aatgatgcat tagcgattgg cgcactgaaa gcgattcaag
    22261 aaaaaggttt agtggtacca gaagatattt ctgtgatggg cttcaatgat gtcagtgtag
    22321 cgaaatatgt aacgccctcg ctttctactg taaaagttta taccgaatgg atgggagagc
    22381 tagcagtgga gacgattctt caattaagta attccaaagc accggtttca agaaagatta
    22441 ttgtggaaac agagttgatt attcgagaat caacgaaata aaagatggaa aagctgtggg
    22501 aaaatggagt gataccgcag cttttttgtg tttcaggaag gggcaaaatt tgcccgccag
    22561 cagataatca tgtatcattg tactagtttt gcctttggta ttttcatcct cagaataaaa
    22621 aagtatgcta caatggaagg cgagaatgtc atagcagaaa ggatcaaata tatgattaca
    22681 ttaggaatag atactgcaaa ccaaactttg gcgatcggtg tggtagaaga cgaacaaatt
    22741 cttggacaaa tacaaacaaa tatcaaacgc aaccacagtg taacactgat gcctgcaatc
    22801 gaccaattgt ttgcagatct tcaaataagt ccaaaagaca ttgaccgaat cgcggtttct
    22861 gatggtcccg gttcttatac agggcttcgt ataggtgtaa cgacagcaaa aacgattgct
    22921 tatacattag ataaagagct agtaggagta tctagtctga aaaccatcgc ggctaattgc
    22981 atcggtgtgg ataaaattat tgtgcccatg tttgatgctc gaagaaaaaa tgtatacgct
    23041 ggggcatacc gctggaaaga aaatagatta gaaattgtac ttgaagatgt acatatttct
    23101 gcttctgatt tgttcacaca actacaaaag ctgaatgaag ctatttattt cgtcgggagt
    23161 gattgtcaca aattcttcta tgaaataaag gaaatattgc ctagtgcgat gattaataca
    23221 gttccagcat gggatattcc aagtggtgta actgtggctc aattaggtgc tttagctgaa
    23281 ccagttgcag agaaacaagc ttttcttcct cgctatttaa aacgggtaga agcagaagaa
    23341 aaatggttgg agacgcacac acctggagac gaaagttatg ttgaaaaaat ttagagcgta
    23401 tgccagaagc ttttttcaag ataaaatgat ttatacagaa aaaacaatcg aacaaatcga
    23461 tcgtcgttac gtactaagag aattgactac agaagatatc aaagagctgc tggatgtcga
    23521 acgagaagtc tataatggcg aattgccatg gacaaaaagt gcctttatat cagaattgaa
    23581 gtcaccgctt cctcatctat atctaggtat ctttgataat ggaagaatga ttggttttat
    23641 cggttcaaga gcgttcggtt tagattgcca tatcacgaat attgccgttc gaccggcata
    23701 tcagcgtaaa cgaatcggtt cgctgcttat tgacgaaatc gagaactttg ctataatgaa
    23761 tcgctgtgag acgatgtcat tagaagtgcg tatgagtaat caagatgcac aaagacttta
    23821 tcggaagtta ggttttcaag caacaacgat caaaaaaaga tattacactg aaacaaaaga
    23881 agatgcgttg gatatgataa aacttttaga aaatgagtga gattagttga tttgcagaaa
    23941 aaaagatttt gatgaagcgt acttggcaga atgcgtatgg atgattagcg aaaatgctta
    24001 tccacatggt tcaccttgga cgaaaatgca gtttttggca gacatccaac agcctcactc
    24061 tgattatctg cttttagttg aagataacca gatctgtggc tttattggat actcgaaagt
    24121 aatagatgaa gtcgagatca caaatattgc tgttgcagtt tcagaacaag gaaaagggca
    24181 tgcacggcat ttgttacaat tactgattac ggaacaaata aaagattcgc tgcatgtttt
    24241 tttagaagta cgtctatcta atgaagcagc aagaaaactt tatgaatcag aaaagtttcg
    24301 tatattaggt aaacggaaat catattatcg caatccgacg gaagatgcga tcattatgag
    24361 cagaaagtta aaaacggaga cgatgaaatg aatgaagaat tgattctggc gattgaatca
    24421 agttgtgatg aaacgagtgt agcggtcgtt agaaatggaa cagagatctt atctaacatc
    24481 gttgcttctc aaataaacag tcataaacgt tttggtggcg tagttcctga agtagctagc
    24541 cgccatcacg tagaacaaat caccttatgc ttggaagatg cactggtaga agctggtgtg
    24601 tcggcagaag atttatcggc agtcgctgtc acttatggac caggacttgt cggctcgttg
    24661 ctgattggta tttctgcagc taaggctttt gcttgggcac accaattgcc gcttattcct
    24721 gtaaaccata tggcagggca tatctatgca gcccgtctgg ttaaaccatt ccagtttcca
    24781 ttgatggcac ttcttgttag cggaggacat acagaattag tctatatgca agaagatgga
    24841 tcatatgaaa tcattgggga gacaagagat gacgcagcag gagaagctta tgacaaagtt
    24901 ggaagagtat taggtctttc ttacccaagc ggaaaagaaa tcgatcagct agctcatcag
    24961 ggaaaagata attaccattt tccacgagcg atgatccatg aagataatta tgatttcagt
    25021 tttagcggat taaaaagtgc atttatcaat ttggtgcata atgcacaaca gcgtggagaa
    25081 gatctagaca aaaatgattt agcagctagc tttcaagcaa gtgtgatcga tgtattgatt
    25141 actaaaacac ttcgagcttg tcaaaattat ccagtcaagc aactgattgt agctggaggt
    25201 gtagcggcca atcaagggtt gcgtgaagga ttgcaggatg ctttggcaac taaactccct
    25261 gaggtagaac tagtgattcc ccctcttcga ctttgtggag ataatgctgc aatgattgga
    25321 gcagcagcac atgtggaaat gcaaaaaaga aattttgcta gctatcagtt gaatgctgat
    25381 ccaagcttgg ttttatctga ataaagaggt tgtgaaaaat ctgccctgca tcaagtaata
    25441 agaacagccc aggaaaaacg gcttctcatg tttttgacct ggctgttctt attaccgcgt
    25501 atgcaagctt ttgaacgccg tttaatcaag agttcgtaag cctgtcgttt aactctgatt
    25561 cacaaggaac aatttcaaaa aacagctcct catgtttatg aacattatga cttgtgaccg
    25621 aagagttgac aggtacacac cgtttaatcg aaataagggc tgtgacacgc ttttgtcaaa
    25681 gccctatttt ttacagctta ttcatcatta tccgttcctc tgattgccct cactgattaa
    25741 tcgtttattg tcgaggaata tcgaataatc taggtcggat gaggacaaga cgcttcctct
    25801 agttaatcta tcagtatttt ttgcaaacag tgaatattac ataactgaaa cacacataga
    25861 aaatctttga gtcttgccca gtgatctaaa actgccggat ctttcagaag ttttagggag
    25921 gaaaataagc agtgaccctg ata
//
LOCUS       DASOUD010000031        25867 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.031, whole genome shotgun sequence.
ACCESSION   DASOUD010000031 DASOUD010000000
VERSION     DASOUD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.031"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(375..1328)
                     /locus_tag="U4A29_001191"
     CDS             complement(375..1328)
                     /locus_tag="U4A29_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO0680325.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1351..2106)
                     /locus_tag="U4A29_001192"
     CDS             complement(1351..2106)
                     /locus_tag="U4A29_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0680326.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2103..3062)
                     /locus_tag="U4A29_001193"
     CDS             complement(2103..3062)
                     /locus_tag="U4A29_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO0680327.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3055..4005)
                     /locus_tag="U4A29_001194"
     CDS             complement(3055..4005)
                     /locus_tag="U4A29_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO0680328.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4155..4607
                     /locus_tag="U4A29_001195"
     CDS             4155..4607
                     /locus_tag="U4A29_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO0680329.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="U4A29_001196"
     CDS             complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="U4A29_001196"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO0680330.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="U4A29_001197"
     CDS             complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="U4A29_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO0680331.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8159..8968)
                     /locus_tag="U4A29_001198"
     CDS             complement(8159..8968)
                     /locus_tag="U4A29_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO0680332.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8977..12558)
                     /gene="smc"
                     /locus_tag="U4A29_001199"
     CDS             complement(8977..12558)
                     /gene="smc"
                     /locus_tag="U4A29_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO0680333.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12578..13264)
                     /locus_tag="U4A29_001200"
     CDS             complement(12578..13264)
                     /locus_tag="U4A29_001200"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO0680334.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13612..15393)
                     /locus_tag="U4A29_001201"
     CDS             complement(13612..15393)
                     /locus_tag="U4A29_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO0680335.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15419..16333)
                     /locus_tag="U4A29_001202"
     CDS             complement(15419..16333)
                     /locus_tag="U4A29_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680336.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16360..17322)
                     /locus_tag="U4A29_001203"
     CDS             complement(16360..17322)
                     /locus_tag="U4A29_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680337.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17322..18266)
                     /locus_tag="U4A29_001204"
     CDS             complement(17322..18266)
                     /locus_tag="U4A29_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680338.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18267..19271)
                     /locus_tag="U4A29_001205"
     CDS             complement(18267..19271)
                     /locus_tag="U4A29_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680339.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(19539..19781)
                     /gene="acpP"
                     /locus_tag="U4A29_001206"
     CDS             complement(19539..19781)
                     /gene="acpP"
                     /locus_tag="U4A29_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO0680340.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(19903..20904)
                     /gene="plsX"
                     /locus_tag="U4A29_001207"
     CDS             complement(19903..20904)
                     /gene="plsX"
                     /locus_tag="U4A29_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO0680341.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(20953..22989)
                     /gene="recG"
                     /locus_tag="U4A29_001208"
     CDS             complement(20953..22989)
                     /gene="recG"
                     /locus_tag="U4A29_001208"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO0680342.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23106..24788)
                     /locus_tag="U4A29_001209"
     CDS             complement(23106..24788)
                     /locus_tag="U4A29_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO0680343.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(24840..25202)
                     /locus_tag="U4A29_001210"
     CDS             complement(24840..25202)
                     /locus_tag="U4A29_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO0680344.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            25472..25660
                     /locus_tag="U4A29_001211"
     CDS             25472..25660
                     /locus_tag="U4A29_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO0680345.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7080 a         5648 c         4550 g         8589 t
ORIGIN      
        1 cttattgccg agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa
       61 aatatctaaa tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga
      121 ggcgatcttg atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat
      181 caacccgtat gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa
      241 accatccgta aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat
      301 aggctcttgc tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc
      361 ctctgttatt tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat
      421 tctaaaccgc ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt
      481 ttaccagcat tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct
      541 ccaccaatag cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca
      601 tattcataag aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca
      661 aagccaaata aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc
      721 ccttcattca caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc
      781 tgatcgattg cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag
      841 atctccccta aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa
      901 gaaaggaaaa tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca
      961 gaaatgatga ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt
     1021 ccacctgctg attctacttt tttataggat gaaagatact caggaaggct gcttgtagct
     1081 gctccaacta ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg
     1141 aatacgacga ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt
     1201 atcgtcaatg tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg
     1261 ttgctatttc cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat
     1321 tttttcatct cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc
     1381 aatctcacaa atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat
     1441 ttcttgggtc gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc
     1501 ataacttgca gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg
     1561 tactaaattt tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa
     1621 aggttcatcc agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg
     1681 ttggagttga ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag
     1741 gtgttccagt gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata
     1801 aggaaatcgt ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga
     1861 ttgtcgtagg atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt
     1921 atggtctaaa tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac
     1981 cgttgacttt ccagcaccat ttggaccgat aaaagcagtc aacttctttt caggaatcgt
     2041 taactgtaca tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc
     2101 aatcatagga tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca
     2161 aattcaacga cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt
     2221 cctcctgtca gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga
     2281 taagtagaaa agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga
     2341 cccacaagag ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca
     2401 aaacgatctg tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct
     2461 agcacatgtc gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc
     2521 aaaagatact gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca
     2581 tattcattcg gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct
     2641 aaaatcatcc ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag
     2701 aaaaataaca atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta
     2761 cctgtctgct gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg
     2821 acatataacg actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc
     2881 tgaaagctga ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc
     2941 ttactcctca atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt
     3001 cctgtaacaa ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc
     3061 atatcgtcgt ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat
     3121 cacactgact ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac
     3181 taagaacaaa ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata
     3241 tattctagag acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat
     3301 caacatgact gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta
     3361 atctattccc agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc
     3421 aaatagccaa accaagagca acaatggaac aacagcatac aataactcat aatttcctcg
     3481 tatcacagtc gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg
     3541 ataagcaaaa aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg
     3601 aagcatcaat tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc
     3661 tgcaaaagca aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa
     3721 aatcatagcc actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga
     3781 aacaaattta ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc
     3841 cacaaggatc aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg
     3901 cccatctaac tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat
     3961 actacttaaa ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct
     4021 aattgagaat gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt
     4081 tcatatgttt taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa
     4141 ggagggtatt ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg
     4201 gtacaccttt agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct
     4261 gctatcttag tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa
     4321 aagaactcaa aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg
     4381 tccaaaacca atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg
     4441 attacgtcac tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa
     4501 atgaacctgt gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa
     4561 aagaaaaacc aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac
     4621 ataaattttt acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt
     4681 caaaagcttt ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa
     4741 tcttctgcct tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc
     4801 ttcgatgatc ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat
     4861 caaatcgata ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt
     4921 gtaagcttca gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga
     4981 gttctccctt agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg
     5041 ttctttcaac atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg
     5101 attcgttcca cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat
     5161 actgtcacca ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat
     5221 cacaaagaaa cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc
     5281 acgaaaagca atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat
     5341 cgtgatacct aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa
     5401 tctcggataa gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg
     5461 gaacccagct tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac
     5521 gatatagctg tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa
     5581 ttgcagacgc aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat
     5641 ggcatcgaac ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata
     5701 gggacgttca tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg
     5761 actcgatagc tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat
     5821 ttgagtcaat gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat
     5881 ttgcttcatt ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg
     5941 ttgttctagt gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc
     6001 ttctttttct tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc
     6061 tggattgata cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg
     6121 aacacctgtt gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata
     6181 aaattctttc tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata
     6241 ctgtttcata gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact
     6301 gttgcgaata ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc
     6361 actcattggt gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct
     6421 cgctgttttc aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg
     6481 gctgaacaat gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac
     6541 catctgtttt cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa
     6601 attttttaat tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact
     6661 taatggccag atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc
     6721 gagatactga aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt
     6781 ttcgctaaaa tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg
     6841 atcggttttt gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa
     6901 tcgagcttgg tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat
     6961 cattaagtga cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa
     7021 cgaaattgtt tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc
     7081 tatgttaaaa acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt
     7141 agacatgggt tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg
     7201 atcaaacggc tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg
     7261 caattcatta cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa
     7321 aacaagaccg gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg
     7381 accagtggtt gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg
     7441 gattactcgt tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc
     7501 tgcagtatca actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa
     7561 tacgacagct gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc
     7621 gccccagaca actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa
     7681 tactttttta ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc
     7741 tacaccatta acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc
     7801 atttacttca ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt
     7861 ttggacttct gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt
     7921 gattcgtatc gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc
     7981 aaaaaagtct tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa
     8041 tgttttacgc gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt
     8101 tttctcttcg ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct
     8161 actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca
     8221 cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag
     8281 catattggat catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt
     8341 tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca
     8401 taaattccaa aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg
     8461 gtatttcttt gattttagga tctaattctt cttggtcata agcgattact gccttattaa
     8521 gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca
     8581 acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag
     8641 acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg
     8701 atacttccat cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc
     8761 cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg
     8821 ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt
     8881 gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt
     8941 ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt
     9001 cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg
     9061 catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac
     9121 gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc
     9181 ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat
     9241 aatggaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag
     9301 actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc
     9361 agttggttcc gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat
     9421 tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc
     9481 catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt
     9541 tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac
     9601 tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat
     9661 atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg
     9721 cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt
     9781 ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag
     9841 cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt
     9901 taatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac
     9961 ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg
    10021 ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa
    10081 ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc
    10141 tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt
    10201 ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta
    10261 atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc
    10321 aaaacgttca agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa
    10381 acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc
    10441 ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa
    10501 ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg
    10561 tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac
    10621 cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc
    10681 taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac
    10741 ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc
    10801 cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg
    10861 ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg
    10921 ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag
    10981 ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac
    11041 cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg
    11101 tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc
    11161 ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga
    11221 tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac
    11281 tgatgcttct acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat
    11341 atattgacgt tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc
    11401 aacatattga tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa
    11461 ttcttgctgt gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc
    11521 tgctgctttc atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc
    11581 tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa
    11641 aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg
    11701 atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga
    11761 ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg
    11821 tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac
    11881 atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc
    11941 ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt
    12001 gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac
    12061 accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc
    12121 tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa
    12181 ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt
    12241 ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact
    12301 attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc
    12361 aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac
    12421 ccagcggata gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt
    12481 gacgctattt tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc
    12541 aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt
    12601 ccgccgcagc ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat
    12661 catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca
    12721 atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat
    12781 gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga
    12841 atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata
    12901 ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca
    12961 aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga
    13021 tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat
    13081 actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg
    13141 ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca
    13201 aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt
    13261 ccattttcca cattcctttt atggatattt tttcgatact tccttattat actggttttt
    13321 tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg
    13381 acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg
    13441 gctttccata tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt
    13501 ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa
    13561 atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg
    13621 tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca
    13681 gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa
    13741 tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc
    13801 aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg
    13861 agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt
    13921 attccaagca gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa
    13981 agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca
    14041 ttgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa
    14101 gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt
    14161 gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat
    14221 ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa
    14281 acctgcatag aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg
    14341 taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt
    14401 ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt
    14461 atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc
    14521 atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat
    14581 ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc
    14641 tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg
    14701 atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata
    14761 tgaccagata ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc
    14821 aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata
    14881 ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt
    14941 attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga
    15001 ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag
    15061 cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg
    15121 agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc
    15181 tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc
    15241 attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga
    15301 gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat
    15361 cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact
    15421 agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac
    15481 tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta
    15541 agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca
    15601 agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg
    15661 tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa
    15721 aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg
    15781 tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa
    15841 aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg
    15901 gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt
    15961 agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc
    16021 cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt
    16081 catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt
    16141 tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga
    16201 tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa
    16261 attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt
    16321 ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg
    16381 gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa
    16441 aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag
    16501 ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga
    16561 agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg
    16621 actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga
    16681 tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc
    16741 ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc
    16801 accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa
    16861 aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta
    16921 gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg
    16981 cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat
    17041 ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc
    17101 ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc
    17161 tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa
    17221 tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg
    17281 cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct
    17341 ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca
    17401 tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg
    17461 tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag
    17521 atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg
    17581 tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat
    17641 aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga
    17701 acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca
    17761 acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta
    17821 tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct
    17881 tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc
    17941 tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa
    18001 cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg
    18061 gctgttacct tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac
    18121 tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca
    18181 tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg
    18241 tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa
    18301 tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct
    18361 tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga
    18421 ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt
    18481 ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac
    18541 agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca
    18601 gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct
    18661 tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc
    18721 gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca
    18781 cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta
    18841 ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc
    18901 tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat
    18961 gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc
    19021 gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg
    19081 ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat
    19141 tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca
    19201 tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg
    19261 ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa
    19321 ggacattaca cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa
    19381 ttattaataa ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag
    19441 aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg ataaaaagct
    19501 gcatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg gctgctgata
    19561 taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga aatttccgta
    19621 ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc agcttttaaa
    19681 tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa atgatttgaa
    19741 ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac ctccaagaat
    19801 catacacttg tcaattacct acaccatggc taattttact ggcatttttc aggaatgtcc
    19861 accttttttt caatggaacg gatcgcttca gatatccaaa tactattctt cgcttttttc
    19921 aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga tttggcggat
    19981 cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg gtgctttcaa
    20041 gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca aagaacgtaa
    20101 gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta gaatagcaga
    20161 ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa cagcgttgcc
    20221 tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac gagcttctac
    20281 gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt ttttcgtcac
    20341 ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag gacgtccgat
    20401 acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca aatgttctgg
    20461 tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt ctttccctac
    20521 tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc ctacgatgaa
    20581 caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat ctgcttcacc
    20641 gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc gaattgcttt
    20701 tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga tgtttttttc
    20761 atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt ctacttctgg
    20821 aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt gatctccgcc
    20881 cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca gtataaagta
    20941 tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga ggctttgcgc
    21001 taaaggctga tactcaggaa gcatctgcca atcctttatt tgccaaaggg ctgcagcctc
    21061 ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg caaattgagg
    21121 cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca gatctttttc
    21181 gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca cacctagttc
    21241 atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc caacacgtcc
    21301 acgaagttgg tgcagctgtg ccaatccaaa tcgatccgca tccatgatca gcatgacggt
    21361 cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata tctgcatctg
    21421 gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt taccatgtaa
    21481 caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga tttccactgc
    21541 attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat acatttgatg
    21601 tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag gtgttcttac
    21661 ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag atacatccat
    21721 ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca taaataagac
    21781 atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa aacggtgctg
    21841 ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga tcaatgcatg
    21901 tgtgccgatc acgacgttaa tttccccttg ctctagtttt tcaaggagct cacgtcgttc
    21961 ttttgctttc gttgaccccg taatcaatgc gatggttact tcattgggat caaacagctg
    22021 ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca acgcaccttg
    22081 gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt ttccgctacc
    22141 tacatctccc tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc gacagatttc
    22201 attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag taaattgttt
    22261 caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt ctgctttttt
    22321 caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac gtcgtttggc
    22381 ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag aagctcggtc
    22441 aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca atgtgccgta
    22501 ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg atttgttgac
    22561 gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca tcccgttcaa
    22621 ggctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata aaatgacttt
    22681 ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt cttgcatcat
    22741 acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag gcgagactac
    22801 gattcctttc aaagtaacct tttcttgatc ctggatctcg tttagatttc tttcctgaat
    22861 atcttcgtag cgaaaaggat aataagtcag taagtcttca atcgtttgga tgcccaagct
    22921 ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg gatcatctaa
    22981 agaacgcata aatttttcct ccctccttta acaaaaaaag gacttggtca gcgatcatac
    23041 ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat attctgacac
    23101 agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg gtggatctcg
    23161 atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc ttgttcaact
    23221 gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat catttgttct
    23281 agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt tccatcgatc
    23341 atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc tctgatcgcg
    23401 tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc tttgttttct
    23461 tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga aatcgttctt
    23521 gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc tgccatgaag
    23581 atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg aatcgcttta
    23641 acaatgtctt ccgtacttgg attcattgtt tgtccaccac tgataacata attagcacct
    23701 aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat accatacggg
    23761 atacgtggta tttctttttt cgcaaactcg ttcacttctt catcgtgttc caaaatcgtc
    23821 tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa tttttgtcca
    23881 taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat ctcgtcatca
    23941 ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc ttcgtaatcg
    24001 aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt acagtaacca
    24061 aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc tgcattgacc
    24121 atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa ttctccattc
    24181 aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact gtctacgaca
    24241 ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg ctttgctcct
    24301 tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt cttttctcca
    24361 gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt cattactgct
    24421 ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcagaagc atttaatgag
    24481 acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat aacgccggaa
    24541 tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc tcctactttt
    24601 tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt catatttgtt
    24661 cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc tgcatttact
    24721 tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact tatttctgtt
    24781 acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca gtctcctgcc
    24841 tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc agttacgcct
    24901 aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac ttcagagatt
    24961 ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc ttcttggcgg
    25021 acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt atctttaatt
    25081 tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc accaacaact
    25141 gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat ttttacagcc
    25201 atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat attttatcat
    25261 agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc aaaatttcag
    25321 gtataacctt gtttaattta caattattct tctcaattgt caattgtatc tatgaaaatg
    25381 tacttgcaaa ttcccattct atatgatatg ttagtctggt atgagctgaa ttattctatt
    25441 gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact ttacaggtcg
    25501 caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac gtactgttaa
    25561 acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag tttgggtgtc
    25621 aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata cttggaccgt
    25681 tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact tttgattcct
    25741 accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt tattctaaat
    25801 tttatttttt tgtgaaaact agactctttt taagcagaga aaaacaagga tatgataaac
    25861 tgaatac
//
LOCUS       DASOUD010000032        25516 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.032, whole genome shotgun sequence.
ACCESSION   DASOUD010000032 DASOUD010000000
VERSION     DASOUD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.032"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            150..278
                     /locus_tag="U4A29_001212"
                     /pseudo
     CDS             150..278
                     /locus_tag="U4A29_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            392..631
                     /locus_tag="U4A29_001213"
     CDS             392..631
                     /locus_tag="U4A29_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680346.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            743..937
                     /locus_tag="U4A29_001214"
     CDS             743..937
                     /locus_tag="U4A29_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680347.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1023..1694
                     /locus_tag="U4A29_001215"
     CDS             1023..1694
                     /locus_tag="U4A29_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO0680348.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1747..3723
                     /locus_tag="U4A29_001216"
     CDS             1747..3723
                     /locus_tag="U4A29_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEO0680349.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3738..4490
                     /locus_tag="U4A29_001217"
     CDS             3738..4490
                     /locus_tag="U4A29_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO0680350.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4506..5261
                     /locus_tag="U4A29_001218"
     CDS             4506..5261
                     /locus_tag="U4A29_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680351.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5274..5534
                     /locus_tag="U4A29_001219"
     CDS             5274..5534
                     /locus_tag="U4A29_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680352.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5531..7621
                     /locus_tag="U4A29_001220"
     CDS             5531..7621
                     /locus_tag="U4A29_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEO0680353.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7648..8112
                     /locus_tag="U4A29_001221"
     CDS             7648..8112
                     /locus_tag="U4A29_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680354.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8109..8351
                     /locus_tag="U4A29_001222"
     CDS             8109..8351
                     /locus_tag="U4A29_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEO0680355.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8369..8671
                     /locus_tag="U4A29_001223"
     CDS             8369..8671
                     /locus_tag="U4A29_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680356.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8741..10804
                     /locus_tag="U4A29_001224"
     CDS             8741..10804
                     /locus_tag="U4A29_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680357.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10804..13416
                     /locus_tag="U4A29_001225"
     CDS             10804..13416
                     /locus_tag="U4A29_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEO0680358.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13413..15932
                     /locus_tag="U4A29_001226"
     CDS             13413..15932
                     /locus_tag="U4A29_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680359.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAAEMADNDKSGLSDLQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLSKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKS"
     gene            15984..16316
                     /locus_tag="U4A29_001227"
     CDS             15984..16316
                     /locus_tag="U4A29_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680360.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16317..16910
                     /locus_tag="U4A29_001228"
     CDS             16317..16910
                     /locus_tag="U4A29_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680361.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17073..19097
                     /locus_tag="U4A29_001229"
     CDS             17073..19097
                     /locus_tag="U4A29_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEO0680362.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIIVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            19097..20302
                     /locus_tag="U4A29_001230"
     CDS             19097..20302
                     /locus_tag="U4A29_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO0680363.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            20318..20962
                     /locus_tag="U4A29_001231"
     CDS             20318..20962
                     /locus_tag="U4A29_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680364.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            20973..21779
                     /locus_tag="U4A29_001232"
     CDS             20973..21779
                     /locus_tag="U4A29_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680365.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21801..22031
                     /locus_tag="U4A29_001233"
     CDS             21801..22031
                     /locus_tag="U4A29_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680366.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTAERK"
     gene            22500..22808
                     /locus_tag="U4A29_001234"
     CDS             22500..22808
                     /locus_tag="U4A29_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680367.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22810..23232
                     /locus_tag="U4A29_001235"
     CDS             22810..23232
                     /locus_tag="U4A29_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680368.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            23252..24841
                     /locus_tag="U4A29_001236"
     CDS             23252..24841
                     /locus_tag="U4A29_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680369.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24853..25188
                     /locus_tag="U4A29_001237"
     CDS             24853..25188
                     /locus_tag="U4A29_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680370.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            25329..>25516
                     /locus_tag="U4A29_001238"
     CDS             25329..>25516
                     /locus_tag="U4A29_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680371.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9496 a         3961 c         5250 g         6809 t
ORIGIN      
        1 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
       61 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      121 taagaaaccg ttaggtttag ctaagtggtg agtagttcac aatgacgctt acgaatgata
      181 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      241 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      301 agataaaggc ttagaagaag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      361 gagtaaggaa ttcatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      421 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
      481 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
      541 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
      601 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
      661 tttatttttg caaaaaacaa ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa
      721 cagaaaaaaa gtgtcgaaat aaatggaaga aaagttgcta aaaacgatga aacaaaaaca
      781 ccttaaaaga ttatctgtca tgcaatatat caatgatatg cagataacag gaaaagagaa
      841 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaac
      901 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
      961 taaaactttt tctatggaaa tgagaaaaag attttttgtt ttgtctgaaa gggggtagct
     1021 ggatgagaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
     1081 ttggctatgt tagctaccaa aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa
     1141 agatgcccgt agatcaaatc aatgcacatg caaagagtta taaggaggat gcaacgaacg
     1201 tcaataatga tatgtctttg gggcaaatgt tgtccataca acaagaagca atcgaaatgg
     1261 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccaa
     1321 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgaag
     1381 atgctgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa atgccaggtg
     1441 ttttattttc tgatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     1501 atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acctgagtac tttaaattaa
     1561 tcgatggcgt tcctgaagag aactctttct tgtctttgcc aaagaaagga gagaaaccct
     1621 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     1681 gagaactaca ataacaaggt tttaatcaac gtgagtcgtt gaaatatgaa atgaggagag
     1741 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttattgg aatgttagga
     1801 atcattgcag gctgctttca gggatatcga gcatatgcag aagatgtcac gcaaaaaacg
     1861 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     1921 aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata tcctacaggt
     1981 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     2041 gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga cgagattgta
     2101 caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caacaaaatt
     2161 acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc ctatgtcaat
     2221 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     2281 aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc aggattttta
     2341 aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga attaacgaaa
     2401 tttggtgacg atggcgcaga ccaaagcaaa caaacccttt taaaaggggc aaaatttgag
     2461 ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa
     2521 ggaaagctga ctgcgactga tttgacgtta ggaaaatact attttgtgga agttccttcg
     2581 gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt aggcgcagac
     2641 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     2701 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     2761 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     2821 catattccaa cagacattgc aggtggcgca gacggtatca cagtaaacgg tgtgaaaagt
     2881 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaggg actttcttat
     2941 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
     3001 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
     3061 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
     3121 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
     3181 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     3241 gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac aggaatcaaa
     3301 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatggt
     3361 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
     3421 aatgcagcga tcctaaaatc agataaagaa ggtcactttg aaatcactgg tttaactgaa
     3481 ggtgaatatt cactggaaga aacaaaagcg cctgaaaact atcaaaaatt aacgaaagag
     3541 atctctttca aagtagacaa agattcatac aaagaagaaa accgaatcac gatcaaaaac
     3601 aatcaaaaag cttccgtacc aatgaccggt agcaacggtt ttcaaacgta tgtattaatc
     3661 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
     3721 tagaaggatc aaaaccaatg attatcagga agcctaagaa aaagcgaatc atgggtaaat
     3781 ggatcattgc tttctggtta ctttcagctg taggtgtctt acttttgatg cctgctgaag
     3841 cgagcgtggc gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg
     3901 cagcagaaac ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta
     3961 ttcttacaat tctaagtatt tctttaaaac taccaattta tgacggaacg tccgacaaaa
     4021 ttttagaaaa tggtgtaggt attacggaag gaactggtga tattacagga ggaaacggta
     4081 aaaatccttt aatcgcaggt catagtggac tttataaaga taatcttttt gatgatttac
     4141 cttcagtaaa aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc
     4201 aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact
     4261 tagaatcaaa tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca
     4321 acacacaccg ttttctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag
     4381 atgaagaaaa taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc
     4441 tttcagtcat gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa
     4501 ccaacatgag aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg
     4561 cctacacaca gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa
     4621 tccaaacgtc agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag
     4681 ttgattcttc agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc
     4741 acgttcaaaa gattatttct tcagaacaat tagacaacca ggggcatgaa aaacaagaag
     4801 gagtaaatgg cgcgaagttt tctgtttatg atgtatcgga cattttacaa aagatggatg
     4861 ttaaggatct cactactgac caaattgaat cccaattaaa aaatcgagtg aaaaaactat
     4921 ccagtgatca attgaagcta gtttctaatg aagaaacaaa gacaattgat caacaaacag
     4981 gtgtctttga attctcggta gaagtacagg caaatcagaa acaagcttat tatattgtca
     5041 atgaatcaag tcctgaaaac atttctaata gcgaggatat tttgctatta acacctgttt
     5101 ctgataaaaa cggtgaattc ttaaaggatg tgtggatcta tcctaaatca gaagcttcgc
     5161 aacctaaaga agaagtaaaa aaaatcgttt ctactggcgt aaagaaaaac ttctttgaga
     5221 attgttggga ttttgttact cacttgtttt ctagagatta aggaagaaat aaaatgaaac
     5281 tgatcaaatt attattattc aatttttctt gtttaaccgc agtctttttg tactgcggtt
     5341 tattattttc taaactatct attttgccaa gctaccaact atacctaatg aggattttaa
     5401 acataccagc tgcttccatg ccgctagata ttatggaaaa gctcattagg aatccattat
     5461 ttactacgat caatttgatc gttattgtcg gaatttcact agtcatttat aaaaaagagg
     5521 ggatgaaggg atgaatttta aacactcgtg gacaagacgg ttaaaatatg tttttcccat
     5581 aatgatgatg ttaggtacat tgtttgggtt aaaaacagcc aatgtctttg ctgaaaaagt
     5641 tattgtcgat ccgtcaaatc caatccagaa cgtaaagaat aattttatta ttccaaaaga
     5701 tgttcctaat gcaaaaacaa atattactgt aaacggagcg tcaaaagttt acaaatatca
     5761 tgttgatgcc aacaaaggtg gttttacaga cgactacgta ggattagtgg agggaaacaa
     5821 aaatatacgc tatccttctt tccaaaaaga atacggtgaa acaaaccttg ttttagaagg
     5881 cgtaacaacc aacaccaaag tcaatattga ctatggaaaa atcggtacgt ataacgggaa
     5941 aaaagtcaat atcaagctag ttctatctaa tattcactta tactcggata cgttgccgtg
     6001 gaatctacta gataacaact atacaaaaac tcattttcga gacgatggct ataaaaatac
     6061 gaatggtgca atgagtaaat ctaaaaaaag aacagtactt tggatctcag acaatctttt
     6121 ctcaggaatt gtttaccatt caacacaaat gaatgtgcag ctagtagcca catatgagga
     6181 cggtagcccc gttcaatttt caggagatac atttatcagt tttaactctt tgaaccctgc
     6241 aggaggaaaa agcacggatc ttaaaggaga atatgcccac tatgacaaaa tgaatacgac
     6301 agattggtat gtcagaaaag atacggttct ttcagaattt aaatctttct acaacaatct
     6361 gaacgtggta ggtggccacc caggaggaag ctctaagctg acacaagcgg ataatgattt
     6421 caataatttg catgataaat taggagatcc gaagtttggt caaggtactg tgtcctttaa
     6481 aatatctgaa gcaaatccga cgtttgtgat tggttcttct aatgttcaaa cctggtttac
     6541 tttatcttct gcgacaatct ttagtgttgt tcctgatcaa cctgaaaaaa caggggttga
     6601 taaaaatgga aataacgtga acgataaaat gttgcaagta ggagacacga ttcaatatcg
     6661 aatcaaacag aaggtgaatc gattaggagt ggatttacta gcggtttacg atcgatttga
     6721 gttaatcgat aatttaccaa aagaagtaaa ttacgtcgat gcacacgttg aaagtggtac
     6781 aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac accaggtaaa
     6841 atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg aaacttacga
     6901 attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca
     6961 gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg tttatttccc
     7021 taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac gaaatgacgg
     7081 taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat atctagtcac
     7141 acaaaaatgg cacacaaaag gcgtagacgc tgcgtcagat cactacaaac aatttagtat
     7201 tcaagatcca attgaagctc gtttgacata taaagaaggt tctgcgcaag taatagataa
     7261 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
     7321 gctgaagtgg gaagcttccg ctgacttctt aagtaacaat cttttagatg gacgagaaat
     7381 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
     7441 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
     7501 taacttgcca caggtcattg ttcctaaaac aggttctaca catttagtaa cgatttcagc
     7561 ggtcagctta ctattacttg tgttagcaac ttttagttat gcggttctga gatacattta
     7621 aaataaaaaa acgaaagaat gaataaaatg aaaaagttaa taatagtagt tacttccttg
     7681 attgccatga ttgttatttt agtcggatgt agtaatgaaa aaaataaaca aactaatcaa
     7741 gataatggcg tattgaaatc aggaactatg tggaaagaag aagtaggtgg tcttgtttat
     7801 aatttgaaaa ttatagacga gactacctgg gaatactctg agagtgtatg gcatccagat
     7861 cctgtacaaa taacagtcaa gaggcaaaaa gactataaag gtttagaaag atacaaaata
     7921 gtagattcag ctggtgttag ggaatttata aacaaatctg attctttatt tattgttgtt
     7981 ccctatgaga agaatggcgt aaaaaaaata atttttcttg gatctagcaa ggatgaaaag
     8041 cgaacgaaag agaaattgat tcacgatgga tcacaatcca ataaatataa gttacaaaag
     8101 actagcgagt gattgctagt ctttttattt gtcctgaagg aggaatcaca gtggcaaaag
     8161 ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgccgcggt gtatctgaaa
     8221 cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg ggaaaagaca
     8281 aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc gatgaaatta
     8341 ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga attttttaac
     8401 acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaactg ccgcaactag
     8461 tttaaaaaac aatctgatga tttttgctgc tgctgcggcc gttgcttcca ttgttattgg
     8521 gggtcttttc ttcctatttg gacgaagagg agctgaaaat gggaagatgc acgtaggcta
     8581 tgtattgatg ggggtaggag ttattttagc agcagcttct attgtggttt cgttatttgg
     8641 attgtttgga caaacacctc aaggattata agaaagtaaa aaagtaggag taagaagtag
     8701 tcgatacttt ttactccttt tttatgaaag gagaatactc atgtacttta cgattgcagg
     8761 caataccaaa acagataaag agttaataaa aaaagcaacg aaggaagtag gaaagcgtac
     8821 ttctttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
     8881 gttagggaaa gtactggaac acgaaagtcg atcttctgtt aagttgactc tcacgttaca
     8941 tgacacagaa ggaaaaatag agaaagctgg tacagaatac gtttttggcc cactggatta
     9001 tcaaatttca gaaggtttct atcctacatt gatggattta atccgccttg atgtgtttaa
     9061 agaaacggat gaaactatga gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt
     9121 tttagaagaa gagttagaga gcgaccaaag aaaagtgaat caagggagtg aagtcgaagt
     9181 aaatgaagaa gtaccatggg aacgaccaat atttccaaag aaggagtcaa aagaaaaaga
     9241 gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga
     9301 gaacgatgtg atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
     9361 gacggccgaa gtagaagttg atccagttga agcgaatgaa gagtcaacat ggactgaagg
     9421 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagaaaaaa acgaaataga
     9481 gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg
     9541 gatgaaagaa aaaaacaaat tatttttaaa tgagctttat tcgaaatatc aattacccca
     9601 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
     9661 tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt
     9721 attagccacc tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac
     9781 ggaacagtcg tctaattggg agaaagcaaa agaggaacta aataaagaag aacagcgagt
     9841 gattgaagaa gaagtacaaa aaattaaaga gcgatacgaa aagaaacgcg acaatgcgta
     9901 ttccttgtgt gtagcaaaga tcaataattt tactcagacg aatcaagaag cgttatcgaa
     9961 agaaaaagaa aacttgttaa aagaacgtat taaagaacga aaagaagaat tttaccgagg
    10021 tttgcgtaca gataagctaa agattagcaa agaattaaat gaacaactga ataagctata
    10081 tcaaacaacg actgaagttc ttatggaaaa atacgaaaag atccagcaag aattacaagt
    10141 gcaaattcca aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga
    10201 agaaaaacaa cggcaaaaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca
    10261 ggatcttgcg gaacaacaaa tggcaataga aaaacaacgg ttaaaccaag aaaaggaaaa
    10321 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagctcagtt
    10381 aaattatcta gcacaacctc aaccaacgcc ttcagtagct gctgttcaaa ccccaatgaa
    10441 caaccaaaca aatctaactc cttcaccaca gcgttccttt aacggatgga tggtgggatg
    10501 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
    10561 tcaatcttca caggctgatt ctgtggccac aacgacgtca atcaaggaag aagcccaaga
    10621 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    10681 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
    10741 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    10801 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
    10861 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
    10921 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
    10981 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
    11041 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
    11101 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
    11161 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
    11221 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    11281 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    11341 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    11401 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    11461 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    11521 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    11581 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    11641 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    11701 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    11761 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    11821 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    11881 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    11941 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    12001 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    12061 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    12121 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    12181 ggctataaag gattttgtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    12241 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    12301 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    12361 ccagaattag aaaaagaagc cgttctaaca ttagatgagg agaaaagtaa cttattagta
    12421 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    12481 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    12541 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    12601 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    12661 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    12721 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    12781 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    12841 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    12901 gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc
    12961 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    13021 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    13081 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    13141 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    13201 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag
    13261 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    13321 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    13381 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    13441 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    13501 aagtcttaca agattatggt aattatctgc agtatcaaga tgtatttagt tggatttgga
    13561 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    13621 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    13681 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    13741 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    13801 ttatggccat gatcttgatt tttggtggga gttctttaat cgatgaaggg ttaaatatca
    13861 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggatcacctg
    13921 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac gttactagca actgacccga
    13981 ataaagtaga atccctacca acagataaaa gaaatgcttt aacagcgaaa aattttaaaa
    14041 cagcgaatat caatgggatt attactcctg atcaagccgc tgagatggcg gataacgata
    14101 aaagtggatt gtcagattta caaaaggatc ggttaaaggc attacaatac cgattggaac
    14161 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    14221 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatctta gctggtgaat
    14281 cttctttggc ggtggcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    14341 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggccaac
    14401 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    14461 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    14521 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
    14581 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagtaatt
    14641 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    14701 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    14761 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    14821 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    14881 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    14941 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
    15001 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    15061 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtt aatgcagcag
    15121 taaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    15181 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    15241 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    15301 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    15361 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    15421 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta tcaaaacgaa
    15481 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    15541 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    15601 atagtcgttt gaatcaattc aaggataatg atcaaagttt tgttcatcaa tcacctgcag
    15661 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    15721 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    15781 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    15841 ttactgcacc aacaaaaata aatgtaacaa gagaaactaa aaacaggaac aatgaattga
    15901 aagaacaatt attgaaggat atcaaatcat aaaaggccta cgagttaggc cttttttcta
    15961 tgtatgaaaa ggagaaatca tttatggata aatatattaa catgaaaaat ccaccaagat
    16021 ttagtgggga atggaagttt ttcgcctttt tctttttaga tctattaacg attggtggtt
    16081 gctttttttg ggcgaaaata gtcaataatc ttgtccactt accactcttt tttgcactct
    16141 tgaattatgg cttatggatt gcgatagctt tagtgattgt ttggcgaccc tataataacc
    16201 caggtcaacg ccagattttt gcgattatcg agtctatttt ttttacggac aagaaccatt
    16261 atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat gaataaatgc
    16321 gagtcaaagg tcaaaattca gttgacagaa aatccatttt gcagtttgga aaaccagaag
    16381 agcgctctat cttggactta tttccttata aatcctgtac agaagaggga gtcttaatta
    16441 ctaaagaaga tcattttcaa cgttattatc gtgtagtttc tacagatgtg gaaggattaa
    16501 atgaacaaga gaaactggaa cgaatgaatc agttgaccac tgtcatgcgt acgtatgtgc
    16561 caacgattaa gttagttagt ttgaccacag aaacggatct aacggagcaa atcgtgcaaa
    16621 agcggcagtt attagataaa aatcgtatgg aacagtcctt aggaagaaat ctacagcgat
    16681 taagaaaata tgaaaagaaa ttagtagagg aaatccagga attgaaacga gcagaacagg
    16741 aacgccctga tttaagcttc tttttcgtca ttgaaggaaa gacaatcaag gatttgcaaa
    16801 ccagaaaccg tcagttaatg cgatctagtg gcattttagg gttaaaacca ctctacaaaa
    16861 aagaattgat caagattctt tatcgaatga ataatatgaa tgatgagtag gaatggggaa
    16921 gagagaatga aatatccgtt gatatggtta gaaaaagatg ttccgaaaga agagaccact
    16981 agaaaatttc ctcacacttt aagcctgggt aagttgggaa aaggaaaatc ttttaaccaa
    17041 gtaaagaatc aaacaagaag aggtgactgt acatgttaga agcaattaaa acagcttttt
    17101 caccacaaaa tgatcaaaag gtacttgatc cattaaagca actaggaaat cgggaaatag
    17161 aaattttaaa atccaaaggg tatgatttag actttttaaa acaagtgatg ccacaaggtg
    17221 gattaaattt cgatgaagag tatgtgacct atggaaatgg ttattcacga tgtgtgacag
    17281 tttatcacta cacaaaaaat cctacgcttt tttggttggc caatctcatg aacaatcctc
    17341 atacagtggc aacactagat gttagcacag atgaaaagca aaaagtgatt gatcaaatca
    17401 atcgtgcctt agatgaatta aatgatcggt cagataatga acgaaaagcg actgaccgaa
    17461 ataaaagcgc ggaagaatac ctagaactag cggaatatgc ccgaaaattg acttctaatg
    17521 gggaaatttc taaacaagtc aaagtgagag tacatgttta cggaaacacg attcaagaag
    17581 tagatgaacg agcacaggaa ctgatcgaaa atctcaaagg gatggattat cgtgcagtga
    17641 cctatctgca caaaacgaaa agagactggc aatcgatgtt caccatgttc gatgaacaag
    17701 ctgaatggtt tggaaatatc aaagggcaat cactaccatc gctaaacatt ggcggaggtt
    17761 tcccgttcca ccaccaaacg ctaaaagatc caggtggaac acaatatggg gaaaccatga
    17821 caggcggccc gtttgtttgg aatgccacgt ataaaaatag tgtgcggttg tctaccaata
    17881 ccttgcttct aggaatgatg ggagctggta aatcgaccgt tcttaaaatg atcgcggaag
    17941 cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac tttattccct
    18001 ttttaaagga atataacggc attatagtgc gtttagacgg atctgacggg atgatcaatc
    18061 ctttagaaat atttgccaca cgtacgtatg atgaagcttc cagtttatat gacgatggat
    18121 cagtcaaaga tttgaagatc aatgaagcag cgtcctatca aacgcactta gacaaagtgg
    18181 tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca gaatttaaga
    18241 cctatttaaa tcgtttttat gaagagtatg gaacagtacc acgcggtttc acttcctcaa
    18301 actcgcgttc aaatacggag acgcaagtca cagggaaaga tccagaagct tatccgacgt
    18361 tcaaagaatt tttagacttt ttaggacaac tggaattacc aggtgcttcc caagagaaaa
    18421 agaatcgaaa agaagaaatg gaatccatcg tagaatcctt atgtgagacg tatggcatga
    18481 tttttgatgg tcatagtacg attcgacacc ttgaccaaca acagttggtc ttttttgaca
    18541 ttgactctat tacaggttta gatcgtgggg tacaacaatg tcaaatgtac atggctatga
    18601 cgttgatttg gaatcaggca ttgattcaag gaagaagaca acgacgttta attaaagaaa
    18661 aacaaatgga agaacaagac ttcaaatgct tcttggcgat gattgatgag tgccataatg
    18721 cgattaatcc agattttatt gaaacggtaa attatgtcac aaactttcaa cgagaaatgc
    18781 gtaaagtaga agcaatgact gttttagcta cacaatcccc acaagaaatg gtgccagaaa
    18841 atatgtcttc tgataacttc tcgaaactga agaaagtctt tgaattttct tctacgaaga
    18901 tctttatgcg tatggatgaa tctattttac gtcatataga aggattagtt ggtaaatcaa
    18961 tgacaaactc agaattagaa tccattcctc aacaaaatca aggagacgct gtgatcaatt
    19021 ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa ttaaaattgt
    19081 ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta atcaagcagt atgttaagcg
    19141 tcgtgcaaag cgacgcttat ttttgtggtt gtttggtact tctgcaggtt tgattactat
    19201 tttgattact gtgttcgtga ccctttttct aatacttgcg gcgggttcga ttgataattc
    19261 tgattcagat tcaagtagtg gtggcgaagc ttttacagga gaatattcgg aaggcttacc
    19321 gatttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc aatatgctgt
    19381 aggtgcagca gtgaaatata aattattacc aagtgtgatc ctttcgcaat acggttatga
    19441 atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc aactattttg ggattacttg
    19501 gtttgatggt tgtctgtttc ctaaaggaac tgcacgagga attggtggca ttgaaggtgg
    19561 ctggtacatg aagtttccca actcaaaagc tgccttctca tattacggct ttatggtagc
    19621 cacacaatcc aattttaatg catgtgtagg aaataaaagc cctggcgcaa gtttattgat
    19681 cttaggacga ggcggttatg ccgctgcagg aattacggaa gatagtccgt attatacagg
    19741 atgtatgtcc attatcactt ctaataaatt aaccgaatat gatgaattcg ccatcaaaca
    19801 ttggggtgaa ggtggcaata acaacggcac gatcactggt gaatggacga atccattccc
    19861 tggcagcagt ttagataaaa gttccttcag cggtggtcaa ttatttggaa cgaatccagg
    19921 cggtgaattc cgtccgaacg gcttccatga tggattagat tttggttcag ttgatcatcc
    19981 aggaagtgag attcatgcgg ttcatggcgg aaaagtcgtg tacgttggca atcctggtat
    20041 ttcaggtttg ggcgcgtgtg tgatcgtgat taattacgat ggcttgaata tggtgtatca
    20101 agaatttgca aatagtacag gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg
    20161 tcaagtaatt gggatacgag atacagcaca cttgcattta ggatttactc gaatggattg
    20221 gcgtcaagca caaggacatg cattcattga tgatggtaca tggatcgatc cgttaccatt
    20281 tttgaatagc agtaaaaaat agatcaggag aaaaaatatg gaaaataaaa cgttaaaaat
    20341 cgtcttaaca gctgcaatta gcgttagttt aatcggcaat attgcgttgt tttggcagct
    20401 gaaagaggaa caagcagcaa atacctataa aattgaagaa gtggctaaaa acgcgtctga
    20461 gaagctcaaa aaaaattcct ctgaggcaca ggcgaagatc gacaagttaa atcaaaccat
    20521 caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga
    20581 ggaagtaact gaagcacaaa gaaagacggc agaagagttc ggtcaattgg ccattgataa
    20641 atcattaagc agaaatgaat taacggaaaa actaaaagat gtcgcaaccc aagaagtgat
    20701 cgacaaatta agtcctacag acgatgatca ccaacatgat gattacggaa gttactcctt
    20761 tacattaggt gacgtgcaaa cttacgctca aacaagcagt gtaaaagagg aacaaaattt
    20821 tgtcgtattt gttgattata caatcggcaa tccgcaattc aaagatgtca aaccacaaaa
    20881 aataaaaggt ggattaactg tcactgaaaa acaagtagat ggtcaatgga aagtcataga
    20941 ctttagttat ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga gaaagacaac
    21001 gtttcaaaaa acgaaaaagt tatcttcaat ggctgggaat ctttatgatt ctcggctttt
    21061 tgattcttgc tagtccgatt tatacgaatc agaaggtaga tataccagat gaggtaagca
    21121 cgaacgcctt tttaaacacg aaagagctga caatggtttc aaaagaaaaa ggagcgaacc
    21181 agcaattagt ttttgaattt gcgatcgatg atgaagacca aagcttgtta aaagaactgg
    21241 ctaatcttaa ttatcgcgta gaagtaaaga cagctaaagg ggattatcaa gcaatacgag
    21301 ccaaagtgat taaggtgtct gatgattatt ttgcagtgaa gctaacgcat gtaccagaag
    21361 aatatatcgc catgcgttta accattattc ctgaaaaaat tgatccaaaa gtggatatgc
    21421 aagaaccaca agatctgatt tattacgtgc acgaagataa ggttaaagat caagtgaaag
    21481 atgaagatta tgaacaacac gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa
    21541 aagcagctca aaaacaaatg gaatctttcc aagcaaccat tgatttgaat gaagaattag
    21601 tgaagaagct aaaagatcaa ttgccttatc aagtcgcaga tgatcaggaa aatactgaaa
    21661 acaagattaa tagctaccag caagaaattg aaacatcgaa aacgcaaatg aaagatcaag
    21721 aagagatcat tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa aagatttaga
    21781 aggacaggtg gagtgaaaaa atgaagcgaa caaacttggc tgacaaagat actttcgctg
    21841 agcagaaagt attgattaaa gcctatctca aagttgtaaa agctaaagaa atatgtcgag
    21901 aagtgaattt atctaacaca acaatatctg agttgaagaa tggtcgaaag aatttagata
    21961 ctttaaaatt cagatcattt gaagctctat atgtagctgc taaaaaatat tttacggctg
    22021 aaaggaaata aaaacaggag gattttatgt gaaaaaagta tatcaaaaat gtttatggat
    22081 cagctatatc accgtaacac aaacatcaag cacataaaac atttaacact cctcctttct
    22141 ggctgaagat ttcaaccaaa caggaagaca gcgtatcaat tatagcatac actttttctt
    22201 aaattcattt ttcatatgac aaaatatttc cttctaatcg acaaagttag aaggattttt
    22261 tattgtctaa atacacaaat aaccttaatt tataaagagc ataaggaaac gtgggaacac
    22321 tttttaaaaa aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taattgtggg
    22381 aacaaaatat ttaaaaatag gatagtgaaa ggatagcaaa ggatagtaaa aggatagaag
    22441 caggatagcg aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata
    22501 tggaagtaag aataagaaat gtagatgaga aaatcgtaag agaaattgat cgaagatgtg
    22561 acgaactaag taaccgaacg ggtaataaat ggagtcgtaa cgattattta aagctactcg
    22621 ttgaaaatga ttttgatcgt cctttgatgg aatataaaca agataaattt gatcaattat
    22681 taaatcagtt aatagataaa ttttcttttg ttcaaagtca taatacaaaa gtattagaag
    22741 aatatatttg tcagaataat caaattatcg aacttttaat tgaacaaagt agaggaggag
    22801 aagtttaaca tggccaaagt tatagacaag catatttatt tttatgaaac acatatagat
    22861 gttctagatg aaattattgc aactcatgca gaggttaaaa actatagtga agccgttcgg
    22921 tttctatgta tgaattatga ttcagaagtg gagaaaaaag taaattccaa tcaagcaaag
    22981 ttgaatgcaa tgggaaaaga aatttctatt ttaaccgaaa tggtttcaga gtttgcggat
    23041 ctccatttga aagatacagg gattttaatt ggtacaaatt caactgttta ccaagaagca
    23101 aaagagcgtg tggaaggcaa gataaaagca aatcaaacga agaaatattc tcctaagata
    23161 aagaaaacaa taagcgaatc agtgactaca aaaagtgaag tatttgatcc aacaaggttc
    23221 ttaaaacgat aaaaattaga aggagaatgt gatgagtaaa tcacctgcag tcattctcac
    23281 ttgtcaattt accttgccca ataaaaaatc tttttcaaca tacattgatt atatgacaag
    23341 agaaaaagcg ctggaagaaa aggaacgacg tacgcccgag gaagaacagg aactgcgtct
    23401 aatcaagaat gctttagagg atttttatat tcctgaagga gaaacttttt cggagagtaa
    23461 gacgggtaaa gaattcagcg aaacgtcgaa tgaagcgcaa tcgttgatga aacatggaat
    23521 caattttgac aagttagaag aacaagattt taccaaatat ctttcttata tgacacgtca
    23581 ctatgcgtta gaacaaaaga aagtactgac gcatagtgaa caaaaagaat accaacgctt
    23641 gaaccaagca ataaaaaaat atcaaacaga gccacacaaa aaagcttcag gaaatcttcc
    23701 tggagttttt tctatggctt cagacgaagt gaaaggaagt gatctgaagg agatcaagca
    23761 aaccttccaa agaggacaac aaaaaggctc aatcatttat caagatgttg tctctcatga
    23821 tcattcgtat cttgaaaaaa taggattgta cgatcccaaa acagatactt tagatgaaga
    23881 aggtttaaaa gcagctggaa gaaaaatgat ggagacattg ttcgagaaag agcaactgaa
    23941 tgaaacaggc tactggatgg caacgattca ccgaaatacc aaacatatcc atattcattt
    24001 tgcagttgtt gaaaaaaaaa atacgcgtaa accatacact gagattgaaa atggtgtgag
    24061 ttatatcgta ccgaaaggga agagaaggca agcaacatta gacgaaatga agactaatta
    24121 tgtacatgag ttagaacgtt tttcttatga aaaaaatcga atgggtatgg agaaacgaaa
    24181 tttattgaca cggaaaagtg atttgcgtaa caccttaacc aaagtagtga aagagccagt
    24241 acgttatgat cgacacgcaa tgagcttatt aactgaagta tataaaaact tacctaaaaa
    24301 gcgttcggaa tggcattacg gggatgaaaa tcgaaccaaa ctttcaccat acacaagaga
    24361 gaaactcgat gagttaaccc aatatgtgtt aacaaaagaa cctggatatc aagaatatat
    24421 acagatttca aaagcattga aagaagaagc aaaaagtctt tatggggaaa gcaagagaga
    24481 atcaaaggat gcagaaaaaa atgctctctt tgatttaaaa aagcgcacag gaaatgccat
    24541 tctaacagaa ctgaagaaac aggatagtca aataaaacct ttaatagaaa ggatggatca
    24601 gatgcaatct atttacaatg aacgatcctt tcgaacactt aaacagttgc caacgaatga
    24661 atacaaatat agttacgaag aattcatgaa gcgcaggaaa caattccaac aaagagttgt
    24721 cacgcaacgg agtttgcgtc aattaaatcg aacgattaat gcccatgaag aaaaatacca
    24781 cgcaagtaaa gcatacgaag agcaacaacg aagaattcaa atggaacaag aaagacagta
    24841 ggaggaatag caatgaaaca atatttagat caatggaaag ttatagaagg atcattaaga
    24901 gaagaacgaa tcgaacaact tcccgattgt ttagaaaaag agcatttgtt tcagataaga
    24961 gaaatgttga taaacgaaca gtttgatcca aatcaatttt tagtcgttga gtacccagct
    25021 actggtgtgt actgttgtaa tcatgtgaag ggtgaaaaat attttatcat tcaagaatat
    25081 gagggcaagt tagcgcctta ttatactact tgggaaatga atgaagaggg gatcaataac
    25141 ttcccgtgta aaagcattga agagtctatt tcactcacag aatgttagtt gttaagtgaa
    25201 taaaagaagt aaaagagtcc tgaacgtgat tgtccaggac tctttttttt cactgaattg
    25261 ttttccgcca attcattgag tggtgcgatt tccgtctaca ttatatcaaa agaaaggagg
    25321 aagaagaaat ggcttattca acgattggag ttgttgttga aaaaagcaga gacaatcttg
    25381 tttttgtgac agaaattcaa acaggcagag cttttgtagt cacagacaaa gcggcaaaag
    25441 catatcaaag tggcgatatt ttaacgttaa atctgactac gaagacgttc gtcgatgcgg
    25501 cagaagatta tccatt
//
LOCUS       DASOUD010000033        25361 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.033, whole genome shotgun sequence.
ACCESSION   DASOUD010000033 DASOUD010000000
VERSION     DASOUD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.033"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(135..512)
                     /locus_tag="U4A29_001239"
     CDS             complement(135..512)
                     /locus_tag="U4A29_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680372.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /locus_tag="U4A29_001240"
     CDS             768..1496
                     /locus_tag="U4A29_001240"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO0680373.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="U4A29_001241"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="U4A29_001241"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEO0680374.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="U4A29_001242"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="U4A29_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEO0680375.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="U4A29_001243"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="U4A29_001243"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEO0680376.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /locus_tag="U4A29_001244"
     CDS             4631..6070
                     /locus_tag="U4A29_001244"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO0680377.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6093..7145
                     /locus_tag="U4A29_001245"
     CDS             6093..7145
                     /locus_tag="U4A29_001245"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO0680378.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="U4A29_001246"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="U4A29_001246"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO0680379.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="U4A29_001247"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="U4A29_001247"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO0680380.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="U4A29_001248"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="U4A29_001248"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO0680381.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="U4A29_001249"
     CDS             10664..11578
                     /locus_tag="U4A29_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO0680382.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="U4A29_001250"
     CDS             complement(11711..13276)
                     /locus_tag="U4A29_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO0680383.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="U4A29_001251"
     CDS             complement(13269..14720)
                     /locus_tag="U4A29_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO0680384.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="U4A29_001252"
     CDS             complement(14734..15750)
                     /locus_tag="U4A29_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO0680385.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15970..17058
                     /locus_tag="U4A29_001253"
     CDS             15970..17058
                     /locus_tag="U4A29_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEO0680386.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17112..18638
                     /locus_tag="U4A29_001254"
     CDS             17112..18638
                     /locus_tag="U4A29_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO0680387.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18640..19845
                     /locus_tag="U4A29_001255"
     CDS             18640..19845
                     /locus_tag="U4A29_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0680388.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20102..21079
                     /gene="guaC"
                     /locus_tag="U4A29_001256"
     CDS             20102..21079
                     /gene="guaC"
                     /locus_tag="U4A29_001256"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO0680389.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21343..22767
                     /locus_tag="U4A29_001257"
     CDS             21343..22767
                     /locus_tag="U4A29_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO0680390.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23228..24337
                     /gene="pdhA"
                     /locus_tag="U4A29_001258"
     CDS             23228..24337
                     /gene="pdhA"
                     /locus_tag="U4A29_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEO0680391.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24340..25317
                     /locus_tag="U4A29_001259"
     CDS             24340..25317
                     /locus_tag="U4A29_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO0680392.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8204 a         4317 c         5696 g         7144 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15841 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15901 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15961 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    16021 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    16081 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    16141 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    16201 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    16261 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    16321 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    16381 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    16441 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    16501 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    16561 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    16621 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    16681 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    16741 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16801 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16861 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16921 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16981 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    17041 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    17101 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    17161 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    17221 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    17281 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    17341 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    17401 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    17461 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    17521 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    17581 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    17641 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    17701 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    17761 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17821 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17881 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17941 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    18001 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    18061 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    18121 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    18181 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    18241 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    18301 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    18361 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    18421 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    18481 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    18541 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    18601 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    18661 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    18721 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18781 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18841 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18901 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18961 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    19021 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    19081 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    19141 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    19201 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    19261 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    19321 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    19381 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    19441 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    19501 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    19561 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    19621 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    19681 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    19741 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19801 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19861 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19921 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19981 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    20041 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    20101 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    20161 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    20221 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    20281 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    20341 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    20401 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    20461 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    20521 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    20581 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    20641 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    20701 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    20761 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20821 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20881 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20941 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    21001 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    21061 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    21121 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    21181 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    21241 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    21301 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    21361 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    21421 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    21481 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    21541 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    21601 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    21661 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    21721 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21781 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21841 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21901 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21961 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    22021 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    22081 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    22141 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    22201 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    22261 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    22321 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    22381 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    22441 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    22501 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    22561 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    22621 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    22681 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    22741 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22801 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22861 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22921 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22981 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    23041 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    23101 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    23161 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    23221 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    23281 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    23341 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    23401 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    23461 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    23521 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    23581 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    23641 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    23701 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    23761 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23821 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23881 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23941 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    24001 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    24061 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    24121 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    24181 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    24241 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    24301 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    24361 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    24421 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    24481 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    24541 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    24601 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    24661 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    24721 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24781 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24841 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24901 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24961 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    25021 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    25081 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    25141 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    25201 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    25261 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    25321 aagaagggac gaaacaaagt catggcttat caatttaaat t
//
LOCUS       DASOUD010000034        25311 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.034, whole genome shotgun sequence.
ACCESSION   DASOUD010000034 DASOUD010000000
VERSION     DASOUD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.034"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(88..807)
                     /locus_tag="U4A29_001260"
     CDS             complement(88..807)
                     /locus_tag="U4A29_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0680393.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(938..2089)
                     /locus_tag="U4A29_001261"
     CDS             complement(938..2089)
                     /locus_tag="U4A29_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO0680394.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2306..3046)
                     /locus_tag="U4A29_001262"
     CDS             complement(2306..3046)
                     /locus_tag="U4A29_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO0680395.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3050..3373)
                     /gene="rsfS"
                     /locus_tag="U4A29_001263"
     CDS             complement(3050..3373)
                     /gene="rsfS"
                     /locus_tag="U4A29_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO0680396.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3430..4023)
                     /gene="yqeK"
                     /locus_tag="U4A29_001264"
     CDS             complement(3430..4023)
                     /gene="yqeK"
                     /locus_tag="U4A29_001264"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO0680397.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4007..4657)
                     /locus_tag="U4A29_001265"
     CDS             complement(4007..4657)
                     /locus_tag="U4A29_001265"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO0680398.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4667..4978)
                     /gene="yhbY"
                     /locus_tag="U4A29_001266"
     CDS             complement(4667..4978)
                     /gene="yhbY"
                     /locus_tag="U4A29_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO0680399.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4994..6103)
                     /gene="yqeH"
                     /locus_tag="U4A29_001267"
     CDS             complement(4994..6103)
                     /gene="yqeH"
                     /locus_tag="U4A29_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO0680400.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6100..6627)
                     /locus_tag="U4A29_001268"
     CDS             complement(6100..6627)
                     /locus_tag="U4A29_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO0680401.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6807..7592)
                     /locus_tag="U4A29_001269"
     CDS             complement(6807..7592)
                     /locus_tag="U4A29_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO0680402.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7585..8454)
                     /locus_tag="U4A29_001270"
     CDS             complement(7585..8454)
                     /locus_tag="U4A29_001270"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO0680403.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8455..9834)
                     /locus_tag="U4A29_001271"
     CDS             complement(8455..9834)
                     /locus_tag="U4A29_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO0680404.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9842..10261)
                     /gene="fabZ"
                     /locus_tag="U4A29_001272"
     CDS             complement(9842..10261)
                     /gene="fabZ"
                     /locus_tag="U4A29_001272"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO0680405.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10286..10762)
                     /gene="accB"
                     /locus_tag="U4A29_001273"
     CDS             complement(10286..10762)
                     /gene="accB"
                     /locus_tag="U4A29_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO0680406.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10765..12000)
                     /gene="fabF"
                     /locus_tag="U4A29_001274"
     CDS             complement(10765..12000)
                     /gene="fabF"
                     /locus_tag="U4A29_001274"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO0680407.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12019..12756)
                     /gene="fabG"
                     /locus_tag="U4A29_001275"
     CDS             complement(12019..12756)
                     /gene="fabG"
                     /locus_tag="U4A29_001275"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO0680408.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12768..13685)
                     /gene="fabD"
                     /locus_tag="U4A29_001276"
     CDS             complement(12768..13685)
                     /gene="fabD"
                     /locus_tag="U4A29_001276"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO0680409.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13792..14016)
                     /locus_tag="U4A29_001277"
     CDS             complement(13792..14016)
                     /locus_tag="U4A29_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO0680410.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14069..15031)
                     /locus_tag="U4A29_001278"
     CDS             complement(14069..15031)
                     /locus_tag="U4A29_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO0680411.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15041..15481)
                     /locus_tag="U4A29_001279"
     CDS             complement(15041..15481)
                     /locus_tag="U4A29_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO0680412.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15787..16752
                     /locus_tag="U4A29_001280"
     CDS             15787..16752
                     /locus_tag="U4A29_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO0680413.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(16809..17039)
                     /locus_tag="U4A29_001281"
     CDS             complement(16809..17039)
                     /locus_tag="U4A29_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEO0680414.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17125..18012)
                     /locus_tag="U4A29_001282"
     CDS             complement(17125..18012)
                     /locus_tag="U4A29_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO0680415.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18101..19324)
                     /locus_tag="U4A29_001283"
     CDS             complement(18101..19324)
                     /locus_tag="U4A29_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO0680416.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(19341..20387)
                     /locus_tag="U4A29_001284"
     CDS             complement(19341..20387)
                     /locus_tag="U4A29_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO0680417.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(20551..22278)
                     /gene="ptsP"
                     /locus_tag="U4A29_001285"
     CDS             complement(20551..22278)
                     /gene="ptsP"
                     /locus_tag="U4A29_001285"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO0680418.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(22278..22544)
                     /locus_tag="U4A29_001286"
     CDS             complement(22278..22544)
                     /locus_tag="U4A29_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO0680419.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23023..25257
                     /locus_tag="U4A29_001287"
     CDS             23023..25257
                     /locus_tag="U4A29_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO0680420.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
BASE COUNT         6990 a         5525 c         4388 g         8408 t
ORIGIN      
        1 aaatcacaca aaaaagcaag acaatctgtt agtgagattg tcttgctttt cgaaaaagac
       61 tgaatacttt tcgttaaaac tggttgctta gtcttctaat gccacattat ggaacacatg
      121 ctgtacatct tctagatctt ccagtgcatc aatcattttt tcaaatgttt cttgatcttc
      181 tggagatagt gttacctcgt tttgagggat catttggatc tcagcgattg aaaaatcagt
      241 gattcctttt tcttttaatg cttcttgtat actatggaaa tccgctactt caccgtaaac
      301 gatgatttgt ccttcttctt ccattacatc gcgaacatcg atgtcttttt ccattaggta
      361 ttcaaggatc tcgtcagcgt cttctcctgc aaaagcaaag attgctgtat tgtcaaacat
      421 gtaagaaacc gctccggaaa cacccatgtt cccgccattt ttcccaaatg ctgcccgtac
      481 gtctgctgct gttcgattca cattgttcgt caatgtatca acaatcacca ttgagccgct
      541 tggtccaaat ccttcatagc gcaattcaga gtatgtttca tctcctgcac cttttgcttt
      601 ttcgattgct cggtcaataa tatgttttgg tacattataa gttttagcgc gatcaatgac
      661 gaaacgtagt ttttggtttg catgcggatc aggatcacct gattttgctg ccgcgtagat
      721 ttcaatccca aattttgcat aaatccggct attattagca tctttagcag ttttctttgc
      781 tacgatattt gcccatttac gtcccatatt ttcacttcgc tttctgattt gatagtttca
      841 taacgcaaaa acgtcctgtt ccattatact tgtgaatcac gattctgtga agcttttttt
      901 ctttcttcct tcattcttct catttttagt caattatcta tactcggatt gggatttgcc
      961 cgaaattctg ttccggaata ttgcctccca attgatagat ttgatcactt ctaactaaca
     1021 gttccgcttc ttgtgcctct ttttttccga ttcgggaata aatagaaatc tctttttttt
     1081 cttttaagaa tcttcttcca gcatcggtaa atccaagtag tcgaagataa tttttatttt
     1141 gttggacagg aatctcttgc tttttgatat tcagcaagac ataacaagat aatcgttgga
     1201 tccgtgtcca tgtatatctt tttgttttta cctttgacac aaaatcctca aaactatcag
     1261 cagttgcagc tgctgttttc atgcgcgatt ctattccttc ggtcatctga tagatatctg
     1321 tcagttccag caaagtagaa gataacaacc gataacgcaa atacggccaa taagattccc
     1381 aagtctgcag cgaacaagtt gaaagatctt cttctgtctg aggcggcaca agtccagcta
     1441 ttctttttcc ttcttctaac gcatggcgaa tggctgttgc actggctatc ctctgatctg
     1501 tgatgatttt ttcatgatag cctgcacttt tacgtcggat cggatgtaat tccatagggt
     1561 ggacatactt ggcattttcc tttgcataac ttaatcccaa gatatgattt ggcgaagaaa
     1621 aatcaagcgt aatatcggga tacagactgc gaaaaacctc agtcattttt tgcggataac
     1681 tatacgtttg atcagacagt tcttgaaatg cctcttctat cctatcttga ttctcttgga
     1741 caaagcgtcc gaaactgctg taatcaaacg aagtctcttc ttctgttcca aaacacaaac
     1801 tgtcacagtc cagtgtttgc aagattttta ctgcaccttt agcaaaatag tcagctgact
     1861 gaaccgccca ttcgacggga agctccacta ctaaatccac accattttgt aaagcttcat
     1921 tcgcgcgatg ccatttatcg atgatagccg gttcgcctct ttgcaaaaaa ttcccgctca
     1981 ttacagcaat gacgacctct gctcctgtta tttttcgtgc catttccaca tgatagcgat
     2041 gtccattgtg aaaaggattg tactctacga taatcccgca gcttttcatg cgcatcctcc
     2101 ttcatagtta tcgataaaaa agaaatagag acaagtaaaa aaaggcaaaa atcggaaaaa
     2161 atacccgaac tatataaact attctcgcaa acacaatact tctgcgacga aaagctgcac
     2221 tgcaagagca atgcctatat cgttcggata tataatttat gttgaagatt tattcttttc
     2281 ttgttaactc gagcaggcaa aaccgctagt ctttgtatgc tacaaagaac cagcgctttg
     2341 atttctctga tgctgcttcg tctgtaaaat cagaaaatgc ttcgactttc gaaaagccgc
     2401 tgttttccag cattcttaaa taactttcca atggataggt acgctcttga tgtagttcat
     2461 cttcacgaat aaatcgacca gacccttctt gttcttcaac gaaaaaggta agaaaatgtt
     2521 cgatgctgtg tttctcgctt cctggataac tatcccacaa aaaagcaaaa ttatcccctt
     2581 gataatgata gctatattct ggaaaaacct catctacttg ataggtggag tgtacgtcaa
     2641 ataaaaatcg gccattttct tctaacgctt gatagacttc atcaaacact tgctgtacct
     2701 cttgtctatt tttcatatag cacagcgaat cagaaaaaca ggtaattgcc tgatattgtc
     2761 cgatttctga taaatcgagc atattgcctt ccacaaattg gatcgatgcg tcttcttcat
     2821 atgctcgctg gctggcaatc atcaacatct cttctgaaag gtctaaccct gtcactttat
     2881 atccatcttt tgcaaaggca accgctaatg ctccagtacc gcatgccagt tccaaaatat
     2941 tttttgtgtc ttcttgtaaa tgacgtttcg aaaaagctaa ccattgctga taaagtgttt
     3001 cgtccatcac ttcatcgtaa atgaaagcaa atgtttcata agccatatgt taatccaacc
     3061 attcactaag atcgactagc ggagcatctg accatagttt ttccagatta tagaaatttc
     3121 gttctggcgc atggaaaaca tgtacgatca catctccaag atcgatcaat acccattttc
     3181 cgccttcttt tccttcaata cgttttactt catatttgtt ttcttcttct ttatcaatga
     3241 tttcttctgt aattgcgttg atttgacgtt cgctatttgc tgaacagatc atgaaataat
     3301 ctgccaacaa ggaaacattt cgaacgtcta aagcgataat gtcttctgcc cgttttgaat
     3361 ctgctgcttc aatcgcaatt tttaattgtt ctttactatc tatgatgatt tcctcctaat
     3421 atgagcagct tatttttttg ctacccagta attatatgtt tcgattgttt ttggataaat
     3481 ctgttgttct tgcgcaagca aatgggccaa tgtatgtttt gtttcaaatg ctacagcttc
     3541 gtctagatca gcccatgcaa tggctcgtgc atcttgtacc ccaggaaaat ctcgacctgg
     3601 ttcaatatag tctgctacat aaatgatttt gtcaaggaga ctcatcttag cagccccagt
     3661 cgtatgcacc cggattgcat gaagaatctc tgcatcggtg attcctaatt cccgctctac
     3721 aaacgaagcg ccaacaagac catgccagat tgcattatta taattcaata actccggatc
     3781 aaaaccgtct cttacgatta ccattttgaa ctcctcatct ggtcgttctt ttgcataatc
     3841 atgtgtcaat gcagcaatac tagctttttc aggagaagca ccgtattttt tggcaagagc
     3901 cacggctgtt tcttctactc caagcacatg cttgaagcgt tgttcgctca ttctcatctg
     3961 tattttttgc atcaggtttt ctctagaata agcagtatat tcattactat agtccatatt
     4021 catacaaccc ttcattttga atataatcaa ttactttatc tggaacaagg tagcggatag
     4081 aacagccttc tttgatcttt tgacgtattt tcgtggaact gatgtcgatc tcaggtacat
     4141 ctacccaaat tacaggatat ggagtatccg ttgtgtatcc tggtcgacgt atacccacaa
     4201 agttgaccat agaagtcagc tcatcgattt tataccattt tggcaaatat tctaccatat
     4261 ccccaccgat aataaagtaa taatctgtat ccggattgtt ttgcgtcaat tccttcatag
     4321 tatcataggt gtaacttttc ccaccacgag ctaattcaat cgtttcaatc tgcaaaaaag
     4381 gattgtcttc caccgctaat tccaacatgt tgagtcgatg ttttgcatca atcgtctgtt
     4441 tctcatcaac atgaggtggc tgataagaag gcatcaaaaa tacgcggtca agacctaaat
     4501 tccggccagc ttgttcagcc atcactaaat gtgcaaggtg aacgggattg aatgttccgc
     4561 ccaagatccc tacttgcttt ttcttctcta agaattgagg catttcttca ggaaataatt
     4621 gtgaacgaac aaatgtctta gcttgcgtat tcatcatggt cactccttat attgctttta
     4681 cttcttttga tagcctttga tacttttctt ttgtggaagg tttgtataat acaataacac
     4741 gtccaatgat ttggacgatt tcgcaatcta tttctttttc taatacttga gctgcttctt
     4801 ctgcaatctc atctgtattt tgaagtaacg atactttgat cagttctctt ttttctaaag
     4861 cttcttcaat ttgaacgatc attgctgaat tgatcccacc ttttccgatt tgaaaaatcg
     4921 gctgaagatg atgcgctttg ctacgtaaaa aacgcttttg ttttcctctt aaactcataa
     4981 ttttccttct tccttaaatc aatgctttgc gtttcaatac atctacaccc tttggcgccc
     5041 agcccgcaat tactactggc gctgttacag tgatccagcc tagaccggca aaaacgatgt
     5101 ctgttttttc tttcacagaa aactcaaaac gaacaagctc cggaaatgcc tcgacttcat
     5161 caggacgagg aggctgtaat aatccaccta catgtttttg ataaaattcg tctgcttttt
     5221 ccagtttcgt tcgatgaata tttacatcat ttgacacata agcaataaat gaagcacgat
     5281 tgccgctgat aaaatcaaat cttgcaagtc cgcccaagaa aagtgtttgc ccttcattca
     5341 gctgataaac tttaggtttg atttctttta acggtgcaat gatccgcaaa tcttttttac
     5401 ctaagtagtg cgccatttga tggcgatgga taattcccgg tgtatcaatc aaaaaatgcc
     5461 cgtcatctaa aggaatctcg attttatcca aggtcgttcc tggaaactgc gaagttgtga
     5521 ttacatcttg tactcccgct gtatttttga tgatttgatt gataagcgtc gattttccga
     5581 catttgttac tccgacgata tagacatccc gtccatcgcg gtattcttcg attttttcta
     5641 atagttccgt catctcatga gatttcttag cactagttaa taatacatct actggacgaa
     5701 ttcctgcttc gtaagcacgt tccttcatcc attgtgttaa tttagttttt ttcaatgatt
     5761 taggtaatat atccactttg ttcccaacta aaagtactgg attgtcaccg acaaaacggt
     5821 gtagtcctgg gattagtgaa ccattgaagt caaaaatatc caccacattg acgatcaatg
     5881 catctttttg ccctaattca ttcagtaagc gcaaaaagtc gtcatccgtc aatgagacat
     5941 cttggatctc attataatgg cgtaatctga aacaacgctg acaatagact tccccggaag
     6001 ctaaaccttt ttccaaagcc gtttgcggcg tgtatcctaa ttcaccgggt ttgtttgttt
     6061 gtataacggc accacagccg atacattgta attcttctgt cattcgattc cacctcgcca
     6121 gcccatatca gggtgttttg ctaatagata ggccataatt cttttttccc aaaaacgatt
     6181 aaattgtgtc ttccaactat ctgttgcgat aatgggtttt acaaggatgc tacgtatccc
     6241 tgcacgattg gctccacgta tatcagtcat aatctgatcg cctaccatta ctatctcatc
     6301 atctgctaaa tccaattgtt tctttgctat gttgatccct cttgctaaag gtttcattgc
     6361 tcgtgcaaca tacaaaagat caaatttttc aatcgctcgc gcgacacgtt ttgaattatt
     6421 gtttgaaaca acaacaacag ggattcctgc atttttcatt tctaaaatcc aagtaagcaa
     6481 ctcttctgtt ccatctggat tattccatgc aatcaacgta ttgtctaaat cggtcaatac
     6541 tgctttgatg cctagattct ttaattgggc aggtgtaatc tgatagatgg cttctaccat
     6601 ccatgttggt ttgtaatttg aaaacatgct gtctcctcat ctgcaaaaaa tgaaggagca
     6661 cataaagtac tcctcaaccc cgtattatac tgcatatcag caaaaaaaac tacctctata
     6721 ctcgttcgtc tttcagaaaa agcatttctt tacaaaatga ccaaaaataa agattgatca
     6781 cagaaaaatt tagaaacaaa aaacaactaa tattggcgga acctcttgta tcgtttttcc
     6841 agtaatttat gctgatctaa tcgaacaagt tccgtcaatc tttcaatcaa cgcttttttc
     6901 ataatgtgtg cgactttata tcgtggcaac tgctcgccgt tgaacgtttc aggaataact
     6961 cgatcaacga ttcctaactc gcgtagttct ggcgcagtaa ttttcatcaa ctcagctgct
     7021 tctgcggcgc gactaccgtc tttccacaaa atcgacgcaa atccttctgg agaaagcaca
     7081 gcataaatcg tttcttccag catccagact tcatccgcta aacctagagc aagggcaccg
     7141 ccacttccac cttcaccaat gatgatggcg atgatcggta cctttaaatc agacatttcg
     7201 agcagatttc tggcaatagc ctctccttcg ccgcgctctt ctgctcctac accgcaatat
     7261 gcacctgctg tattgatcaa ggtaatcacc ggtctattga acttttccgc ttgtttcatc
     7321 agtcgtaaag cttttcgata gccttcagga tggggagaac caaaatttct gcggatattt
     7381 tcctgcagat cccgtccttt ttgaataccg atcactgtta ccggtttgcc atctaatcga
     7441 gcgattccac caacaatcgc ttcgtcatct gcaaataaac gatcaccatg gcattcctcg
     7501 aaagaatcga agatctcttc aaaaaaatcc aagcttgtcc atcgttcttt agctcttgct
     7561 aactgaacga catcatgtgc tgtcttatcc acgatctatc catcctttct gtgtatgcaa
     7621 ttcaagaagc gtatggattt tctgtttcaa ttccatgcgg ggaacaatct gatcgacaaa
     7681 accatgagat aataagaatt cagccttttg gaaatcttct ggaagttcct gcttgatcgt
     7741 ctgttcgatg acgcgtcttc cagcaaaacc gatcaagctt tggggttctg ctagaataat
     7801 atcgccttcc attgcaaaac tggcagttac tcctccggtc gtcgggtccg ttaaaacagt
     7861 tagataaaaa agaccttcat tgctatgccg tttgacagca gcagagattt ttgccatctg
     7921 cattagagaa aagattcctt cttgcatccg tgcgccacct gaagctgtga acaagacgac
     7981 tggaagactt tcctttgttg ctcgttcgaa taatctggtg atcttttctc cgacaatcgt
     8041 tcccatactt cccatgataa aattaggatc catcactcca ataatgaagg gaatcccctg
     8101 aatggttgct ttaccagtga ggactgcttc atgaagtcct gttttatctt gcattttttt
     8161 aattttttcc gtatattcgg gaaatgctag cggatctttg gttacaagat ccgtatccca
     8221 ttcttcaaaa cttttttcat caataatcaa agctagacgc tgccatgcac cgattcgaaa
     8281 agcgtatccg caatgagggc atactttttc ttcaccgatg tctttcgtat aaaggatatg
     8341 tttgcaattt ggacactttg cccacatgtt atcaggaaca gatggcgagt tagcaggttt
     8401 gctctctcta ttaggattga tccgaatata tttctttttc ttaaacagag ccatctatgc
     8461 gcctcctatc tccgtttctg gtgtccaatt cggtaaaaat tcttcttgta aaaatgctgt
     8521 gctgtaatca cctgctatga ctgcaggatg actaatcaaa tccatctgaa attctgcgtt
     8581 cgtgatgatt ccttctgtga taaattcact taaggctcgc tgcatcttca ttaatgcttc
     8641 aaaacgcgta tttccatgga cgattatttt tgcaatcatt gaatcataat atggcgggat
     8701 cgtatatccc gggtaaactg cactgtctac tcgcagtccc atcccacctg ctggaagcag
     8761 taagttttgg atcttccctg gagaaggagc aaaatggaaa gcaggatttt ctgcattaat
     8821 ccggcattcg attgcgtgtc cttggaatac tacttcttct tgacacacat tcaaaggctc
     8881 accggctgct atctccacct gtttttttac taaatcgatt cctgtgacca tttctgttac
     8941 tggatgttcc acttggattc gagtattcat ttccatgaaa taaaaatcgc cttcttcatc
     9001 catcagaaac tcgatcgttc cggcattttc ataattgact gctttagctg ctcgaacagc
     9061 agcgtctcct agtgcttctc tttttgtttg tgagatcact acagaaggag attcttccag
     9121 tactttttga ttgttgcgtt gtaaagaaca atctcgctcg cctaaatgaa tgacatgacc
     9181 aaatcgatca cctaagatct gaacttctat atggcgagca ggataaatga ttttttccaa
     9241 atacatatca tcatttccaa atgccgctgc tgcttcttgc tgagctgaag aaaaatgctg
     9301 tgggagctct tcttttgatt gaactttccg aatgcctttc ccgccaccac ctgcagctgc
     9361 tttcagcatc actggataac caattctgtc tgctatttcc aatgcttctt ctactgtaga
     9421 taatacacca gtacttcctg gaatgactgg gacatttgct tcttgcatca attgacgcgc
     9481 attgatctta tttcccattg catcgatcgt tgcactttta ggaccaataa atgtgatatt
     9541 gcactcttca cacatggagg caaatcggct attttcagat aaaaaaccaa atcctggatg
     9601 aattgcttcg gcttttgtca cgattgcagc gctcagtact tcctgaacat tcagatatga
     9661 atcaacggct tttgccgggc caatgcagat tgcttcatct gctaactgtg tatgaagtgc
     9721 ttcggcatct gcttctgaat atatcgctac ggtttggatc cctagttcgc ggcatgcacg
     9781 aatgatacgt acagcgattt caccgcggtt agctatcaat actttagaaa acatatttca
     9841 cctatccaat cataaaggtt aattccgctt cggctacttt tttaccgtct actttagcga
     9901 ttcctttgcc tattccggct gaagctttca ctttcaatat ctcaacttca aggtataagg
     9961 tatctcccgg tgtaactttc ttacggaatt ttgctttgtc caatccgccg aaataagccg
    10021 tctttccctt gaattgttca agagaaagta atgcaactgc tcctgcttga gccattgctt
    10081 caacaatcaa tacccctggc ataacaggtt cttctggaaa atgtccttga aaaaagggct
    10141 cattgatcgt tacatttttc ttagcaacga tccgttctcc ttcaaccatt tcttccactc
    10201 gatcaatcaa aagcattgga taacgatgtg gaatgatttc ttttatttct tgtatgttca
    10261 ttgttttccc tctttcctgc tttgcttatt tttgctgaat cctaaagagt ggctgtccat
    10321 attcgacaac tgactcgttt tctacaagga tttctgtgat cactccgtcg gcctcactag
    10381 ttatctcatt catcagtttc atcgcttcaa taatgcagat aacttctcct ttggcaactt
    10441 gatcacctac ttttttgaaa gctggtttgt cgggcgctgg ttgcagataa acgattccca
    10501 cgattggcga aacaacctcg attccatcaa atactgccga ttctttttct ttcattattt
    10561 ctggagcagt tgttgcttgt tcagatatga cattgactgg ctccgaaact ggcaacgttt
    10621 cgtttgtttg aaccatattc atgcctcgac ttgttttatt tttattcata tacaattcaa
    10681 attgaccttc acgcaaatcg aattccgtta gcgaggattg atcaaattga ctgactaatt
    10741 ctctgatttc attgatatcc aaaattattc ctcccagcgt ttcatgcaca gaaccgcatt
    10801 atgtccgcca aaccctaatg aattactcat tgcataagta aaacttgcag gtttgctttc
    10861 gtttttgaca actgtcaagt caatctcagg atcttgtttt tctacattga tcgtaggtgg
    10921 gataaattga tgttctaatg ccagcaaagt tgccaaggct tcgatcccgc cggctgctcc
    10981 caataaatgt ccagtcatcg atttcgtgct gctgacgtaa gtatctctga aattttcacc
    11041 taatgcgatt tggattgctt ttgcttctgc ctggtcattg gctggtgtac ttgttccgtg
    11101 agcattgata tatccgactt gttccggctg tacaccagct tcttccatcg caagcttcat
    11161 tgctttagat gcaccagaac catctggagt aggagctgtc atatggtaag cgtcacagtt
    11221 tgcaccgtat ccaacgattt ctccatagat tttggcaccg cgagcttgtg catgttctaa
    11281 agattcaaga actagaacgc ctgctccttc acccatcaca aaaccattcc gattttcgtc
    11341 aaacggaatc gaaccacgat taggatcagt tgattctgag agtgctgtta aagctgcaaa
    11401 tccagcaatc cccatctcac aaatcgttgc ttctgttcct ccagcaagaa tgacatcaga
    11461 atagccatgt ttgatgttac ggaatgcttc cccaattgaa ttcgttccgc tagcacaagc
    11521 tgttacgata gctgtacaaa taccttttgc acctacacgc aaagcaatat ttccagctgc
    11581 catgttggca atcgccattg gtacgaacat tggagaaact cgttttgcac ctttttcatg
    11641 tagacgaata acttggtttt cgattgttgg aagaccaccg atcccagatc cgatgatgac
    11701 gccaaaccgg tcttgattca ttttttcttt atctaaaccg ctcatctcca ttgcttctaa
    11761 agcagcgtag attccataga tcgaaaacat atccattcgt ttgctatctt ttttaataaa
    11821 atatttatcg taagggaaat ctttgacttc acccgctaag gtaatccctg tttctgaagc
    11881 atcaaattta gtgatcggtg cgatgccatt cgtcccctct tttaagctat tcaaaaagct
    11941 gtctgcgtca tttccaatag gtgaagtgac tccatagcca gtaattacca ctcgattcat
    12001 ttgtttgctc tccttttctt atccgtgcat gaccaatccg ccatcgacat tgatgacttg
    12061 tccggtgata tatggatttt gtgctaagaa aactgctgtt tgtgcaatat cttctacttg
    12121 tccgaaacgc tgcatcggaa tttgcttttc cgcttgttcc ttgactttgt ctgctaaaac
    12181 ttccgtcata tcagttgtga tgaatcctgg cgcaatggca ttacatgtaa tgcctctagt
    12241 tgcagcttct cgtgccacag attttgttaa tcctactacg ccagctttac tagctgcata
    12301 atttgcttga ccaatatttc caatcaaccc agatacacta gagagattaa tgatggcccc
    12361 ttctcgctgc ttcatcattt ttttcaatac gtgctgcgtc atattgaagg ttccaatcaa
    12421 attgatatcc agacattttt tgaaatcttc tgcatccatt cgcatcacaa gtttgtcatt
    12481 tgtgattccg gcattgttta ctaacacatg gatcgatcca agcttttctt ctgcttcttt
    12541 gatcatttgt cccgctttct cataatcaga gatgtcaccg gaaactccta cgcattttac
    12601 accgaatgct tcgatttctt ctatcttttc ttttggaatc tctccgcgac cattcaaaat
    12661 gatatttgct ccagcttttg caaaagctaa ggccatcgct ttgccgattc ctcttgtact
    12721 acctgtgata aatacatttt ttccttttac atccataacg ttcctcctta tagtattgct
    12781 attgtttcat caaatgtctg cttattctca acacgcagaa ctggtattgt tttatcgatt
    12841 tttttcataa atccgcttaa tacttttccg gggccgactt cgatgacttg ttcgatgccg
    12901 attgttttta acttatggat actctcatag aaacgaacag gttgcatgac ttgctgttca
    12961 agtagtcccg cgattgtttc tttcttcatt atttccgttg ttgtgttgct tatgatagga
    13021 aaagaaggtt cagagaattg aatctgactt aggtccttcg ccagtttttt tgctgccggt
    13081 tctaaaatag ctgtatgaaa aggcccgctg acatttagag ggatcattct tttgaaccct
    13141 ttttctttta atagtaaaac agcttcgtct actgcttttt cttcgccacc aatcacgatc
    13201 tgctgaggtg tattataatt tgcaggagat acgatgccgt acttgctagc ttcatgacag
    13261 ctttcctcga tcgtttcaat cggcgcattc atcactgcaa ccattttccc gcttccagct
    13321 ggggtagctt ctgtcatata agcgcctctt tttgctacta acgcaactgc ttcagtgaag
    13381 gatagcgcat tactagctac taatgccgaa tattctccta aactcagtcc taaagctgca
    13441 tctgggatga ttccatgctc cttcaataag cgataatacg cgatgctgac tgttagaata
    13501 gctggctgtg tatattgcgt ctgatccaaa cgttcatttt ctgtaaagca aagttcagcc
    13561 atatcgtagc cgagaatctc acttgcttca tcaaatgtct gctttacaac ggcttcttca
    13621 tataaatctt ttcccatacc ttgatattgt gcaccttggc cactaaataa gaatgctgtt
    13681 ttcatgtttt tctccaatat atccagaata tatgtttgat tcttcctatt caaaaaggcc
    13741 gagacattcc tatttcagga aatgtcccag cccccaacct aattttaatg attatgcagt
    13801 ttgtttttct acgtaagtca ctaaatcttg aaccgttttg attccgtctt ctgtttcgat
    13861 tttcacgtca aattcatctt caatttcatt gatgatttgg aataaatcca agctgtcagc
    13921 ttctaagtct tcttggatgt cagtagtcaa tttgatttct tcagtttctt ttcctaattc
    13981 ttctgcgatg atttcttgaa ttttttcgaa taccataata atatattcct ccgatattgt
    14041 ttgcttttta tttttataat ttaacagtct acaactccat gagaatactt ccccatgtta
    14101 aacctccgcc atagcctgta aatattactt tctgctgttt tcctaaggtg atctttccac
    14161 tagaaactgc ttcatccaat aagataggga tgcttgcagc agatgtattc ccatatttat
    14221 ccatattggt caggaacttt tcttgcggca tctttacttt tcgggcaatt ttctcaataa
    14281 ttctgaggtt tgcctgatgc aaaagcagat aatcaactgg tttgtctttc atgacttctt
    14341 ggatattgtt tgcaacgtct cgtacggcaa aatcaaaaat atctctgcca gtcatttgca
    14401 aataatacga accttctgaa tcttctgaat agaacggatt tttattttgc tggtagccag
    14461 cggtcaatga gctacttctg gtaccgtcag ctgctaattt ttctttcagc aattttttct
    14521 gtggattcgc ttctaagaga atcccacctg ctccatcgcc aaacaacaca gcagtcgtac
    14581 gatcagacca atcgattagc ttagatagtg tttcaccacc aatcaccagt cccttagttg
    14641 agccgcagcg gatcattttt tcagccatgc ttaacgcata aacaaatcct gagcacgcag
    14701 cactgatatc aaaagcgata gcatgagttg ctcccaattt cccttgtact tgtgctgcta
    14761 cagatggtgt cgtgtaatcg ggcgacattg ttgctactaa aataaaatcc aaatctttag
    14821 ggtttagatt tttctgtttt atcaattttt ccgccactaa aatgcataaa tcagaagtct
    14881 cctgatttgt tacacatctt cgttcacgaa tacccgtcct tgacgaaatc cattcatcag
    14941 aggtatccat catcttggaa agatcattat tagtaataat cttttcagga aggacgctgg
    15001 ctgttgcagt aatcattcca aattgttcca ttaaatatcc tcacttatag tcttgcaaga
    15061 agtcgtgcaa gtttttcagt gcatccagca aggcatgctg ttcctccgta gccattccgt
    15121 ctaagatgtt cttgaccatt tcacgatgaa aatgctgatg gacccgaaat agtaactttc
    15181 cttttttcgt tagtcccaac tttacaacgc ggcggtcatc ttcactgcgg atacgttcga
    15241 catagccttt ttttactaac ttgttaatag cagtggtcaa ggtcccaaca gtaattgata
    15301 gttctttggc tacttctgaa gttgtttttt tcttgtacat cccgattgcc tcgatcgtgt
    15361 gcatctctgt gattgacaga tctttgaatt gagatttttt cagctcagac tcttcgattg
    15421 tcaaaatatc attgaagaca ctaacaagat aatcatttac tgtttttaaa tctggttcca
    15481 tgttccaccg tccactaatt agtttgatta tcaaatcttt tgatgttcaa actatatctt
    15541 aacttttttc aaaatgcaac cttaaaagat cattttttta ctatatgagc tttttttaag
    15601 attttactca tttaaaacaa ctgataaatc agcatttata atttctttta ttagaaaaat
    15661 catctatctc acatattttg attatcaaag tattttctgt gaacagaact atagaattcc
    15721 ctaaaacttg tccattattt caaaatttgt tatagtcttt ttattcaaag cgtaaaagga
    15781 gatcctatgt taaaagaaaa aatgaaaact ctccccatgc tgccgggtgt ctatcttatg
    15841 aaagaccgag atggtaaggt catctatgtt ggtaaagcaa aacggctcaa aaatagagta
    15901 agcagctatt ttcatcagaa taaacagcat tcaaaaaaag tattacgtat gattcatcat
    15961 ataacagatt ttgatttcgt cgtagtagac acggaattag atgctttatt gctcgagtgt
    16021 caattgatcc aacattaccg tcctttttat aaccgacaaa tgaattattt cagtaattat
    16081 aattatgtac atattaccaa taagggattt gtattaacag atactcctac ggctcgaaca
    16141 tacgggcctt ttcgtctcta taaaaaaatg ccgtctattt tgcgtataat ggaagaaact
    16201 tatcaaatgc cttggttgtc tgaaattagt ttgttagctt tgcgagtcca gcttcctgac
    16261 ctacaggaaa tgacttttga acagaaaaaa aaggaactcc aagggctgtt tcaaggccga
    16321 aataaaaagc tactgactta cttgaaaaaa agacaacaac atttcatcta tcagttgaat
    16381 ttcgaaaaag ccggtatgct tcaaaaagat atcgaattag ttacttattt tattagacgg
    16441 atccaggaac aaaagcagtt tttaagaaca ccatcgctca ccttcagcat gccattagca
    16501 gcagatgaga gtcagaaaaa gcactacctc gtttgttatg gtcaacttgc agaaacaatg
    16561 attacctctg gtgacgtttc tcctgacttc tattatgaaa agaaagaagc tcatttatct
    16621 ttgaaacgtc aattgtcaaa agaagaaatc gatcctgtac aaatcttgat cagttaccgc
    16681 aaaaaattag aaaaagaaca gtttgaaatt gaactgttaa acaaaaaaga agctgaaaag
    16741 caactaaatt agttgcttct cggcttcttt taggcatctt caacttcaaa tgtcaattat
    16801 cctaaaattt attggattaa gtcatagatt tccatcgcta cgatatcgat attatcaaat
    16861 tcgtattgct gatgagtatc tgtttcagtt aattcaaatg tatcggtcgc tttatcataa
    16921 gttactgtac acttctctac gccttctttt tcaaaacggc gaacttgcag ttcattatct
    16981 gctgactcga ccatagcctc cagtcgtttt atgattgcga ctaattgtga atgtgtcatc
    17041 ggaggtcctc cttttcttat ttctgttata ttgtatcaaa agcatagcct acttgcattg
    17101 attttctcta aaaaaactcg ttttttatga ttttttttct ggtatttttc cttcaggcca
    17161 ccaaagtttg atcaaagctt gggttccgtc attctcaaaa cctttatctg ccaactcttc
    17221 atatagtttc aatgcttggg tagttgctgg caatacaaga tccatttttt ctgcttcttc
    17281 taatgcaatc tttaaatctt tgataaaatg tttgacaaaa aaaccaggtg tgtaatcttc
    17341 ttttaaaata cgaggagaat aattgcttaa agaccagtta gctgcacttc cacctgaaag
    17401 cgtctcaatc actttcttta aatcaagtcc gtttttttgt gcatagacaa gcatttccgt
    17461 cattcctgtc atcgtacctg caatcatgat ctgattagcc atttttgtat gctgaccttt
    17521 cccagcagaa ccatgcaatg taaatgtcgt accaaattct ttgaataaag gcagtacttg
    17581 atcataaacg gcttcttctc caccgaccat gatcgtcagt gtaccattct ttgctcctaa
    17641 gtcaccaccg gataccggtg catctaacgc atctgcgcct ttttcttttg ctgtttttgc
    17701 aattttttct gccaggcttg gtgtacttgt agtcaaatca actaaaatat gtcctgagat
    17761 atctgcagaa aaaatccccg actgtccata gtagatttct tctacatctt tggggtaacc
    17821 taccattgta aagagaatat tactttgttc tgctaccgct tttggtgtat ctgcccagat
    17881 agctccttct gttactaaag gatcagcttt tgcttttgtc cgattataga cagctacttc
    17941 gtgtcctgct tctagtagat ggcgtgcgac agcacttccc atgacgcctg ttccgataaa
    18001 accaagtttc attttttcga cctcctaaaa atcaaaaatc cttgcttcta tgctagtata
    18061 cgctagaaac aaggattttt tcataaaaaa tgtttgaatt ttatctttta aatgaattta
    18121 attttatttt gattcgttca atcgagtctt cttcttcttt tcttcgcatc tcttcttcat
    18181 gataaacaac catgtcttga taaaattcga tcatagcttc gccgaaatgt gtagatgaaa
    18241 tatcataaag tttagcttct aaaacttttg gatctttagg aacttcactg gaaatataat
    18301 caataagtgc tggtgcaaag tcttcgtcgg tttcaaaagt tttccctaat gaaggatgat
    18361 cgaacaatcg gttcaaataa gcatttcctt ccacgacaca aggtgttccg gctgccatcg
    18421 cttctgtata agtaagccct tgtgtttctg aagtagaagc actgacaaaa taatctgccg
    18481 ctttataata aagtgctaca tcattgttgt ccacttctcc tgtgaactgc acaaattctt
    18541 ctaactgcag ttctgagatc aattcctcta actcttctac ataaggtcct tttcctacga
    18601 taactaatct ggcgttagga taagcggaga tgacctgagg aaacccatta atcaaggctt
    18661 ggatattttt ctcataagaa atcctgctta aagaaagaat catcaaatgg ttttcctgaa
    18721 gacctagtga ctcccttaat ttgctgatat cctgctgagt gatatcagaa cgtttgaatt
    18781 tttcaatgtc gataccagta ggaatcacgc gaagcggtgc tattacacca tagctgcgta
    18841 atgtatcaat aacacgttca cttggacaaa ctacacctgt cgtatgattt gcaaataatc
    18901 tagacaagta ttttacatgg gattgcctga tcacttttcc tttaccgata taatgaaggt
    18961 agtcttcata catcgtatgg taagtatgga tcagaggtat tttcaacttc ttagctacca
    19021 tcttcccaag tatccctgct ccgaattctg tatgggtatg aataagatct agttctagtt
    19081 ctttggcaac caaataagca taccacatgc ctctaacaac aactcttcga tccttgaaag
    19141 aaataaatgg tacacttggc attcgaatca catctttttc caacttatcc gcattaggat
    19201 cggtcgttgt aaaaatatag acttcatgcc catgtctttc taattcttgt tttaatgttt
    19261 tgatcgatgt agcaactccg ctgacttgcg gaaagtaggt atcggtaaaa aagccgattt
    19321 tcatgttttt cgcctccaaa ctagtccttg acctgattta ctttttttaa tttgctagag
    19381 actttttcat tcagtacact ttcgtataca tttttaagtt cttcaccgat tttattgata
    19441 cttcgctctt ttgctgtttg ataaccggct tgtgtcgtac tcggtagttc ttgattggta
    19501 atctgttgga tcagtcggat aaattcttcg tttgtcttcc ccatatagca gttcttgcta
    19561 tcttctaacc agccattata cactggaata tctcgaacaa gtacgttttg tttgcttgct
    19621 aaagcttcta aaacaacgat cccttctgtc tcttcataag aagggaaaaa gaatagatct
    19681 gcacctgaat acgcaccttc tatgatctct ccacgaatgt aaccaggaaa caggacattt
    19741 tcaggatgat cttcttttac aattttccgg atgttccgag gaatcgagta catgggaaca
    19801 tgaccaaacc aaataaatgt gtattcgggc atcttcttag caatttctac gaaatcaatg
    19861 attcctttac gttcgaaaaa caaaccgaca caaatgatta ctttttgatc cggagacagt
    19921 ttgaaatatt ctctgaactt ttgttctttc attggatcag ggtgatattt ctctaagtcg
    19981 atcccattgg aaatagcttg aatcggcaca cggataccat agctttccaa caactgtttt
    20041 gaataaggtg ttggcgtgat cagatagtcc gctttttggt acaatcctac cagatatttt
    20101 tttacgatag gagaaagctg gttcgatccg ataaaggaat ttcggaaatc ttcctctgta
    20161 gaatgcgcat gatagacaac tttttttcct tctcttctgg ctttattgac catattatga
    20221 ctattgattc cgtatgtatt gatgtgcagg atgtcataat cttctttttc atctagtgta
    20281 taagagatac cgacttccgt cagagcacgt ttttgatgat caagcgcacg cccgattcca
    20341 gattttgcaa gcatcttttc actttcaaaa tataataata ccttcacctt acgtccccct
    20401 tcacttatat agtataacat agcctacaaa ctgtcactca aacttaagcc ctatttttgt
    20461 ctattcttac tgacaaaaac gttttcttaa aaaagaacaa aaaaactggc gtagctcttt
    20521 tacaagccac accagctttt gatacgttgt ttattttaca tattcatgaa caagttcaac
    20581 gacttcttcc atcatatcgc attctttcaa tgcacgatca gcaagttcag ccattttcgc
    20641 tgtgtccaag cgtttcatta aactacgcgt tttaagaata gatgtcgcac tcattgagaa
    20701 ctcatctaga cccataccta ctagcaatgg aacagctgtt tgatctccag ccatttctcc
    20761 acacatacct gcccattttc cttcagcgtg tgctgcatca attacatttt tgatcaaacg
    20821 taaaattgat gggttatatg gttggtacaa gtaagaaacg cgttcgttca tacggtctgc
    20881 tgccattgtg tattgaatca aatcgttcgt acctacagag aagaaatcta cttcttttgc
    20941 aaacttgtct gctaagactg ctgctgctgg aatctcgatc atgatgccga cttggatcgt
    21001 atcagatact tccttgcctt cacttatcaa tttttgtttt tcttcttcaa agatcgcttt
    21061 tgctgcgcgg aattctttga gtgtagctac catagggaac atgatacgta aattaccatg
    21121 aacagatgca cgaagcaatg cacgcatttg tgtacggaac atttcgtcgc cttgttcaga
    21181 taaactgata cgcaatgcac gatatcctaa gaatggattc atttcatgag gtaattgcag
    21241 atatggtagt tctttatctc caccgatgtc cattgtacga acaactacag gttttccttc
    21301 cataccttct aatacagctt tgtaagcttc atattggtca tcttctgttg ggaagtctgg
    21361 agaatccata tacaggaatt cagtacggta cagaccaact gcttctccac cattattatg
    21421 gacaccaact agatcttttg gtgttccgat gtttgcagct aattcaaaat gcttgccgtc
    21481 agctgtgaca gtttctgcat tttttaattt ttcccattct gctttttgtg ctgcgtaatc
    21541 tgcacctgct ttttcaaatt cagctttttg ttcatctgta ggatcgatga ttacatcgcc
    21601 ttcgatcccg ttgacagcca aaatatcgcc ttctttgact ttagcagtaa tttcttttgt
    21661 cccaacgatt gctggaattt ctaaagaacg tgccatgata gcagaatgtg aagtacgtcc
    21721 accaatgtct gttacaaacg cttttacaaa attgcgatct aattgtgctg tatcacttgg
    21781 agtcaagtca tgtgcaacaa cgaccacttc ttcgttgatc attgatggat ttggtaaagt
    21841 aacgcctaat aagtgcgcta aaatacgttt agcaacgtca cgaatatctg cagcacgctc
    21901 ttgcatataa gcattatctt ccattgcttc gaacataccg atatacatat ccgttacttc
    21961 ttttagcgct gattcagcat ttacgctgtt gtctttaata ttttgcttga tttgacctac
    22021 catttctgga tctgaaagaa ccatcaaatg ggcatcaaat acttgcgctt ctgcttcacc
    22081 taagctttgc gctgcttttt cacgaatttg ctgcaattct tcagtagatt tagctaatgc
    22141 agcatccaaa cgagcttctt cagccgcagt atcttctact gttgccttac tgaatgttaa
    22201 atccggttga actagcaggt aagcttttgc aacggctaca ccgtcactag cggcaatccc
    22261 ttttagcatt tcaaccatta ttcagataag ccttctttct tcattgtttc aacgattgct
    22321 gccattgcat cagcttcgtc agcaccttct gcagtaatag taacatcaga accttgacct
    22381 acacctaaag acataacgcc catgattgat ttcaagttta ctgatttacc tttatactct
    22441 aagtttacat cagagttaaa tttgcttgct gtttgtacta atagtgtagc tggacgagcg
    22501 tggatcccag tttctgctac tacgtgaaat tctttctttt ccatattaat cggtctcctt
    22561 taagtgagtg ttgtttttat gtgagggttt tcttttttag taattcagag aatcctaaca
    22621 aatgccaccc tttcatctgt aaagattaac attttttttc gttagataca acagttttca
    22681 ttcaatttca gataaaagta ttcaaattat tccggattct ttttcatcct tttcttcaca
    22741 ttttataaca aacgtgttta tttccttttt cttgtcgctt tctcttctcc tgacgtttat
    22801 cattctttcc tttgaccctc tttgttttca tatgaaacga attacttgct tcccttttta
    22861 tttttagtat aataagagtc aaaggtcagg aatggtcaat agagatttca ccttttctac
    22921 ctcataaatc tcttaaaaaa ctaggggtta ttcttttacc tcatgaaaaa cgagatgtaa
    22981 aatatggatg tacccatttt tgtgaaagga cgtgaatgat tcatgctttg tcaaaactgc
    23041 ggcaagaatg aggcaacgat tcacttgtat gccactgtca atggtcagcg gacacaactc
    23101 gattactgtc agagctgtta tcaaaaaatc aaaaatcaac aaaatggagg tctgactaat
    23161 atggcccaaa atgatccatt tggatttggc agtttggacg atcttttccg ttccatgtct
    23221 cgccaaatgc aacagcaagg aaattatgag caaacaccac caacacaatt tggcggaaac
    23281 aacaacttca atggtggtca gccacctcaa ggcaatacag aaggtttatt gggagaatac
    23341 ggaatcaata tcacagaaca agcacgacaa ggtgatatcg atcctgtgat cggacgcgat
    23401 gacgaaatca aacgagtcat tgaaatttta aatcgccgaa ccaagaacaa tcctgtcttg
    23461 attggagaac caggtgttgg taaaacagca gttgttgaag gtttagcaca aaaaatcgta
    23521 gatggtgatg taccgcaaaa attaatggac aaagaagtca ttcgtttaga tgtcgtatct
    23581 ttagtccaag gtactggtat acgaggacaa tttgaagaac gtatgcaaaa attgattgaa
    23641 gagatccgtc aagcagaaaa tgtgatccta ttcatagatg aagttcacga aatcgtcggt
    23701 gctggttctg ctggtgatgg aaatatggat gctggtaata ttttaaaacc agctttagct
    23761 cgaggagaat tgcaaatggt tggtgctact accttaaatg agtaccgcat catcgaaaaa
    23821 gacgctgctt tagaacgtcg tatgcaacct gtacgggtag atgaaccaac tgtcgaagaa
    23881 acgatcagta ttttgaaagg tttgcaaaaa cgttatgaag actatcatca tgtaaaatat
    23941 actgatgagg ccatcgaagc agccgcaact ttatccaatc gttacgtcca agatcgcttc
    24001 ttgccggata aagccatcga tttgctagat gaaacaggtt ctaagaaaaa cttgacgatc
    24061 caaatcgttg atcctaaaac aatcgagaaa aaactgcaag aagcagaaga acaaaaagtt
    24121 ttagcttctc gagaagaaga tttcgaaaaa gcagcttatt atcgcgatca gatcaataaa
    24181 cttcaaaaaa tgaaagaacg tcagctcact gaagaggaaa cacctgtgat taccgaaaaa
    24241 gatatggaaa aaatcgttga acaacggaca ggtattccag tgggtgaatt aaaagaaaaa
    24301 gaacagacac aactgaaaaa cttagcagat gacttgaaag ctcatgtcat cggacaagat
    24361 aacgctgtag atcgagtagc gaaagcgatt cgccgaaacc gtgttggatt aaacaaacag
    24421 aatcgtccaa tcggctcctt cttatttgtt ggaccaactg gtgtaggtaa aacagagctt
    24481 gcgaaacaac ttgcctacga actattcggt tcacaagatt caatgattcg tttcgatatg
    24541 tctgaatata tggaaaaaca tagtgtctct aaattgattg gttctcctcc aggttatgtc
    24601 ggatacgaag aagctggtca attaacagaa aaagtacgga gaaatcctta cagtcttgtc
    24661 ttgttagatg aagtagaaaa agctcatcca gatgttttgc atatgttctt gcaaatctta
    24721 gatgatggtc gattgacaga tgcacaagga agaacggtca gctttaaaga tacgatcatc
    24781 attatgacaa gtaatgccgg aactggtaaa gtagaagcaa acgttggttt tggtgcggca
    24841 cgcgaaggtg ttactcgttc tgtattgaac caattaaaca actactttac accagaattt
    24901 ctgaaccgtt ttgacgggat catcgaattc agtgcattat caaaagaaaa cttgatgaca
    24961 atcgtcacac taatgctaga cgatgtcaac caaatgctgg cagcacagca acttcacatc
    25021 gaagttccta cgaatgtcaa agaaaaatta gttgacttag gctatgatcc atctatgggt
    25081 gctcgtcctc ttcgacgcac gatccaagaa caaatcgaag atggtatcgc tgaattttat
    25141 cttgatcacc cttctatcca tgagttgaaa gcaaaactgg ataaagatgg aaaaatcatc
    25201 gttacttcta aaccagaacg tttggctaaa gaatcagccg aagaaacggc tgaataaatc
    25261 cagttttata gcgtgagacg gtatttcttt gagaaatgcc gtctcattct t
//
LOCUS       DASOUD010000035        24196 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.035, whole genome shotgun sequence.
ACCESSION   DASOUD010000035 DASOUD010000000
VERSION     DASOUD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.035"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            171..320
                     /locus_tag="U4A29_001288"
     CDS             171..320
                     /locus_tag="U4A29_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO0680421.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            331..1845
                     /gene="dltA"
                     /locus_tag="U4A29_001289"
     CDS             331..1845
                     /gene="dltA"
                     /locus_tag="U4A29_001289"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO0680422.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1842..3047
                     /gene="dltB"
                     /locus_tag="U4A29_001290"
     CDS             1842..3047
                     /gene="dltB"
                     /locus_tag="U4A29_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO0680423.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3092..3325
                     /gene="dltC"
                     /locus_tag="U4A29_001291"
     CDS             3092..3325
                     /gene="dltC"
                     /locus_tag="U4A29_001291"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO0680424.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3328..4596
                     /gene="dltD"
                     /locus_tag="U4A29_001292"
     CDS             3328..4596
                     /gene="dltD"
                     /locus_tag="U4A29_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO0680425.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4737..5819
                     /locus_tag="U4A29_001293"
     CDS             4737..5819
                     /locus_tag="U4A29_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO0680426.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5819..6622
                     /locus_tag="U4A29_001294"
     CDS             5819..6622
                     /locus_tag="U4A29_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO0680427.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6692..7432
                     /locus_tag="U4A29_001295"
     CDS             6692..7432
                     /locus_tag="U4A29_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO0680428.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7443..8447
                     /locus_tag="U4A29_001296"
     CDS             7443..8447
                     /locus_tag="U4A29_001296"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO0680429.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8492..10336)
                     /locus_tag="U4A29_001297"
     CDS             complement(8492..10336)
                     /locus_tag="U4A29_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680430.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            10548..11066
                     /locus_tag="U4A29_001298"
     CDS             10548..11066
                     /locus_tag="U4A29_001298"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO0680431.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            11194..11877
                     /locus_tag="U4A29_001299"
     CDS             11194..11877
                     /locus_tag="U4A29_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO0680432.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            12186..12259
                     /locus_tag="U4A29_001300"
     tRNA            12186..12259
                     /locus_tag="U4A29_001300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12220..12222,aa:Arg,seq:tct)
     gene            12418..12777
                     /locus_tag="U4A29_001301"
     CDS             12418..12777
                     /locus_tag="U4A29_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEO0680433.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            12900..14531
                     /locus_tag="U4A29_001302"
     CDS             12900..14531
                     /locus_tag="U4A29_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEO0680434.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(14593..15234)
                     /locus_tag="U4A29_001303"
     CDS             complement(14593..15234)
                     /locus_tag="U4A29_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO0680435.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    15420..15496
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            15516..15824
                     /gene="rplU"
                     /locus_tag="U4A29_001304"
     CDS             15516..15824
                     /gene="rplU"
                     /locus_tag="U4A29_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO0680436.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            15851..16195
                     /locus_tag="U4A29_001305"
     CDS             15851..16195
                     /locus_tag="U4A29_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO0680437.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            16208..16501
                     /gene="rpmA"
                     /locus_tag="U4A29_001306"
     CDS             16208..16501
                     /gene="rpmA"
                     /locus_tag="U4A29_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO0680438.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            16935..19742
                     /locus_tag="U4A29_001307"
     CDS             16935..19742
                     /locus_tag="U4A29_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEO0680439.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            19933..20424
                     /locus_tag="U4A29_001308"
     CDS             19933..20424
                     /locus_tag="U4A29_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0680440.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            20527..21516
                     /gene="manX"
                     /locus_tag="U4A29_001309"
     CDS             20527..21516
                     /gene="manX"
                     /locus_tag="U4A29_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO0680441.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            21549..22352
                     /locus_tag="U4A29_001310"
     CDS             21549..22352
                     /locus_tag="U4A29_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO0680442.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            22373..23287
                     /locus_tag="U4A29_001311"
     CDS             22373..23287
                     /locus_tag="U4A29_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO0680443.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            23370..23744
                     /locus_tag="U4A29_001312"
     CDS             23370..23744
                     /locus_tag="U4A29_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO0680444.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            23883..24128
                     /locus_tag="U4A29_001313"
     CDS             23883..24128
                     /locus_tag="U4A29_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO0680445.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         7765 a         3994 c         5085 g         7352 t
ORIGIN      
        1 ttaaaagtac gagaaattat aaagaaaaaa gcagtgattt gatgtgctat ttttataaaa
       61 gctatgtgac aaaactgtta gtaaaaaaac ggtttctgta agctagacac atgtttcttt
      121 ttgtcaaaat tagttacact ttgtgtgagg ttatatgagg agggaatcca atgaacaaat
      181 ggcgttctaa tccaaaattg aattattggg gaaaattcat cattcgaaca attttttata
      241 gtgcagtact gctgttcctg atttatctgt atcactataa aaatattcaa ggaggaactt
      301 ttatctataa cgaattttag gaggaagagt atggaaatca aaacgattat tgaagcaatc
      361 gatgattggg gaataaatga acctgatcga gttgcttatc aggcggatga gacgcatact
      421 tttggtgaat tgaaacaagc atcagacgca cttgcttatt atttagaaaa aaaggtagaa
      481 ggcgatggac caattgtcgt ttttggcaat ttggagtttg aaatgattgt ctcatttcta
      541 ggggttgtca aatctggcca tgcctatttg ccgattgaag agcatacacc caaggagcgt
      601 atcctatcga tctttcgggt ggctaaacca tctatggtca tcagtatcgg ggactggata
      661 gaagatattc cttcggttcc tgtcataaca aaaaaagaat ttcaagaaat caagaagata
      721 ccagtaggat tttatcccag aaatagcgta aaaggtacag aaaattttta tattattttt
      781 acttctggta ctactggaga accaaaaggc gtacaaatca gtcatgataa tctagtcagc
      841 tttgttcaat ggacgatgga agactttggg atcaagcctg gtatgcattt cttagcccaa
      901 gcgccatttt cttttgattt atcggtattt tctatttatc ctgcattagt ttctggcgga
      961 atgctgaagc cgttaaataa agcagttgtt caagactttc gtcaattatt cgcgacattg
     1021 ccagccttga agttgaatgt gtgggtttct actccatcat ttatggatat ttgcttgatg
     1081 gaacctacat tcaatggaga aaatgtgccg gaattggaca tgtttttatt ttgcggagaa
     1141 gaattgacga aaaaaactgc agaaagtcta cttgatcgat tcccaaatgc acggatatat
     1201 aatacttacg gtccaacaga agcaactgta gctatttcaa gtattcagat tgaccagcat
     1261 gtactagata gttatgaccg actaccgatt ggctacgtaa aaaaagacac gaaagtttct
     1321 attgtccaag aaggtaaacc tgtttcaaaa ggagaaacgg gtgaaatcat tattgcagga
     1381 ccaagcgtat caaaaggtta cctgaacaat cccgaaaaga cagctgctgc gttcttttgt
     1441 caaaatgact tagcaagtta ccatacaggg gatgcaggta gactagatga atatgggctt
     1501 ttattttatg aaggacgtat ggatcagcag gtcaaattac atggataccg gattgaactc
     1561 ggggatatcg aacattatct gctccaagac aaccgaatca aacaagctgt cgtcgtacca
     1621 aaataccaag gaacaaaagt acagcagctg gttgcttttg tcgtattaga aaacaaaacg
     1681 aaacagccgg attttcagtt gactaaatct atcaaagaac aattactaga agttgtaatg
     1741 gattatatga ttcctcagaa gttccattat gtcgatctgc tgcctcagac agttaatgga
     1801 aaaatcgatc gaaaaaaatt aacagctgag gtgaatcctg aatgatgtta cctcatatga
     1861 tcccttatgc agagccgttt tatttttttc ttttagccat tgcgcttgta ccgatcgttc
     1921 tatctttatt gatatgggaa aaaagattac cagtttatca atcagcagtg acacttttct
     1981 ttttatttgt cagctttgga ggtaattatt ggcaacaggg agcggcgctg attgggtatg
     2041 ttttgtggca gacgatactt gtatggggct attttgttta tcggcaaagg aaaaatcaga
     2101 caggatggtt ttttgccgcc gttattttag cgataattcc actgttttta acgaaagtga
     2161 ctcctttttt tgaacatgga aaagcatcgt tacttggttt tttaggaatc tcttatttaa
     2221 cctttaaatc tgtacaaatg atcatggaaa taagagatgg catgatcaaa acatataagc
     2281 catttcgtta tatccagttt ctgctgttct ttccaacaat ctcttctgga ccaatcgatc
     2341 gctataggag atttgaaaaa gatcagctcc atccgcctgt tcgtgataaa tatgttgatc
     2401 ttttgggaaa aggcgtccaa aatatttttg tcggtttttt atataaattc attatcggtt
     2461 attattttgg tcaagtactg cttcctgtag tgggacgcat tgccttacag catggtggta
     2521 tttcatgggc attagtaggt tatatgtacg tgtacagtat gtatttgttt tttgattttg
     2581 ctggctatag cttatttgca gtcggtacaa gctacatgat gggatatcag acaccaatca
     2641 atttcaataa acctttttta tcatggaata tcaaagaatt ctggaatcgt tggcatatga
     2701 cgctgtcctt ttggttccgt gattatgtat atatgcgatt gatgttcttc atgatgaaga
     2761 aaaaattttt taaaagcaag atcgtaatgt caaatatcgg ttattttgct ttgtttcttc
     2821 taatgggtat atggcatgga ttgacgtggt actacatcgt atatggactg tatcatgctt
     2881 gtctgatctg tctgacagat gcatggatcc gatttaagaa gaagcataaa gagcagttgc
     2941 cttctaacaa atttacgcat ggcttagccg tatttttgac atttcaagca gtatgtttta
     3001 gcttcctgat tttttcagga tttttagata aacttttttt tagctaacga atagtgacat
     3061 attaatttat ataacaaagg agaaaaggaa aatggaagaa caaattttat caatcttaga
     3121 agaaataaca ggaacagatg aagtaagaaa aaatcgtgat attgatctat ttgatgaagg
     3181 gttgcttgat tctttagcat ctgttcaatt attagtcgag ctagacggag agctggatat
     3241 ccaagtccct gtatctgaat tcgagcgaga ggattggagc acaccgaata aaatcattca
     3301 acaagcaact gcattgaagg gttgagtatg aaaaagaaac tttttgctat tttcggcccg
     3361 gttgttttag ctgttggctt actgtttgtg ttttttacct cctcctatca agtagatatc
     3421 tctaatccag aaacgattag acgtgcctct tcttccatgt cagtgaacgt gttgaaagga
     3481 gatgcgataa aaaattatgc attatctgaa aaacaatata ttcctttttt tggctcaagt
     3541 gaattaagca gaatcagtcc atttcatccg tctgttctgg cagaaaaata ccagcgaaac
     3601 tatcgtccat ttttgctggg agcacccggg acacaatcgt taagccaata tatgatgatg
     3661 cgttctgcag gagatgctat gaaaaacaag aaagtcgtct ttattatttc tcctcagtgg
     3721 ttcgttaaaa atggtgtaaa aactgattac tttaatacgt attattctga acttcaaaca
     3781 tacgactggt tgttttcaat gaagaaagta acgcctgctg atcgctattt agctagaaga
     3841 ttattaactt tctcaaaagt aaaagagaat gatacgttga cggcgattct gcaaacgatc
     3901 aaaaaaggga agctaccttt acctgaatct ttgaatcagc tgcgcagcca atggaacatg
     3961 ctgaagagag aggatgaagt attcagcaat attgggttga aagatcgaca gcaaaaaatc
     4021 gatcatgagt caaaacgatt accaaaacaa taccaagaaa cagaattatc cattttagca
     4081 aatcaaattg gagaacgaga aacgacgaat aatccattcg ggttgaaaaa cgatttttat
     4141 acgcatcgta tccgagccca cgagccagag ctcaaacagt cacaaaaaaa ttgggattat
     4201 cgtttttcgc cagaattttc tgattttcag ctggtcttgg atcaattagc aaaaaaccat
     4261 aacgaagtat tgttcatcat cccaccggtt aacgagaaat ggagcgatta taccggacta
     4321 tctcaagaaa tgttacaagg atttgccaaa aagatcaaat ttcaattaaa tagccaagga
     4381 ttcaatcgaa tagctgattt tgtcaatcaa gcgggtacca actattttat ggaagacacg
     4441 atccatctag gctggaaagg ctggttggct gcagatcaac agatcagacc gtttttagaa
     4501 gaaaatcata ttactgctag taagtatcat cttgacgatg ctttttttag caaatcttgg
     4561 caacatcaaa taccagataa actacaatta aaataaagaa aacaggcgag tgttgctcgc
     4621 ttgttttctt tatttttgaa tattctgaat aaaagataaa aataccaagt ttgcctgaac
     4681 aaaaatcata agcaatggta taatcacaat atgaatatag aaatggggga aaacatttgg
     4741 atacaaaaga agaacagcta cttattgatt taacttctgc aaaaggagta cctggaaatg
     4801 aagaagaggt aagagaagta ttcagagaat atgccaaacc attcgctgat gatatttttt
     4861 atgacggttt aggcagtgtg attgccaaac atggtgctgg cggaggcccc aaggtcttta
     4921 tttctggaca tatggacgaa gttggtttta tggtaacaaa aataaccgaa aaaggattct
     4981 tggaattcca aactcttgga ggttggtggg ggcaagtcat gcttgctcag caagttgaaa
     5041 tcaaaacacg tgaagggaaa atcgtccacg gtgtgattgg ttccaaacca ccacatgttt
     5101 taacacctca agtaagaaat aaaccttatg aaataaagga tatgttcatt gatattggcg
     5161 catcaagcca agaagaagcc aaagagtggg gcatacgtcc tggagatatg gtcactccat
     5221 atattgaata caaacgaatg aatggcagta aatatttact ggcaaaagca tgggataatc
     5281 gtatcgggac agctgtttct ctgcgagtat tagagaatct atctaaagaa gcacacccaa
     5341 atgttttatt cgcaggaagc gatgtgcaag aagaagtagg cttgcgaggt gctcgaacaa
     5401 gtacacacct tgtaaatccc gatattgctt ttgctttaga tactggtaca gcaggagaca
     5461 ctccaggaat gacaccaaaa gaagcagatt cgattttagg aaaaggccca cagattttga
     5521 tttttgatgc atctatgatc ccgcataaaa aattattgaa ttttgttatt gatgtagcag
     5581 aggaaatgga tattccattc caatatacag tgatttctgg cggaggaact gatgcaggac
     5641 aaatgcattt gacaagagat ggtgttccgt ctgtagccat taccgttcct gttcgttatc
     5701 tgcattccca tacatcagtg attcatgaag atgattattt gaatacagtg aaactagtca
     5761 cagaattagt gaaacggctt gatagtgaaa cagttgaaaa aattcggagt tatgaataat
     5821 gaataataga atacctattt ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca
     5881 atggggaatc aatacttggc ttgttttctt atctataata tttacgagcg tcatccagaa
     5941 cttttttgac ggtttcagtc cttttccgga agaaagcatg cagcagcact tattgagttt
     6001 tctagtcgat aatttacttt tattcgcatt caactatgca ttgttttaca ttgcgctagt
     6061 agttgtacgg ggtggcagac caaaaagtaa ccttttattt tcagttttcc aaagaaagta
     6121 ttttggttcg atacttggca tcaatttgat caattcagtc atcaactggc tactcaacag
     6181 tttaatcttt ctgcccatgt ttttaatcag cggagtgagt acttatatgc agctgctttt
     6241 cagttcaaat gcggtaccta cgggtgtcat gccagaagat tttcttgata tcagctttgt
     6301 tttatcgatg atagggatga tttttgtttc agcactcatc atgagtattg ttggcggttt
     6361 attccaattt gctgcatgga caaagttcga ctatcctgat ttgtcggtta ttcaatgttt
     6421 gaaatatggc tggtttttat tgaaagatag aattggtacg tatattttgt tgcagctgtc
     6481 ttttatcggc tggtatttgc taggagcaat cgctttgttc atcggtcttt tgtgggtcat
     6541 tgtttatgtg aatgtgtcag ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg
     6601 tccagacgcc tattttactt agcatgtaag cagtttattc atcaaaaaaa taacttttgt
     6661 ggtaggataa atctttgagg gggaatgtac gatgaaatta actgttttag gatgcttagg
     6721 agcgtatcct tataaaaatc aagggacaac aggttatttg cttcaatctg acgggttcaa
     6781 tttgttgatc gatgcaggta gtgctacgct gatcaaatta caagaacacc ttgatccact
     6841 tgatctagat gctgtgatcc ttagccatta tcatcatgat catattgctg accttggtgt
     6901 attacagtat tactggcaac ttcatcctaa gagaaatccg gagacattgc tttcaattta
     6961 tggacacaca aaagattcca ttcactttga agaattgacg ttggaaaatg tctcaaaagg
     7021 acatccttat tttgaagctg aagaattaaa aattggtcct tttctgatta cttttatgga
     7081 aacaatccat ccagtagtat gttacgccat gcgtatcgta gaagaaacaa cggggaaagt
     7141 attcgtattt acaggtgatt caggttattt ggaaagtttc gctgattttg cgaaagatgc
     7201 tgatttgttt ctagcagata cctatctttt tgaaggaaat gaacatcatc atgcccattt
     7261 cacttcaaaa gaagctggag aacttgcgaa atcagctaac gtaaagaaac ttgtacttac
     7321 acacctacct cagtttggtg atttagaaca attgaaaaat gaagcgcaaa aggcagcagg
     7381 caaagggatt gatatagaac ttgctgcagt tgacaaagtg tttaacattt aggaggaaga
     7441 acatgatttt tgtaccaaat gaaaaccacg acccaagaat caatttagca attgaacaat
     7501 atttgctgca agaaatgcct ttagatgaac ctattttgct cttttatatc aatgaacctt
     7561 caatcatcat cggcagaaac caaaatacgc ttgaagaaat caatcgagat tatgtggaag
     7621 aaaatggcat tcatgttgtc cgtcgcttaa gtggaggcgg agctgtctac catgattttg
     7681 gaaacctgaa ctttagtttt atcatgcctg atgatgggga ttcattccgt gactttgcga
     7741 aagtaacaca gccaatcatc caagcacttc atgaattggg tgttgctggg gcggaattga
     7801 aaggccgaaa tgatctagtc attgatggca tgaaattttc tggcaacgcg atgtatgcta
     7861 ccaacggtcg gatgtttgct catggaacaa ttatgttcga ctctgatatc aatgaagtag
     7921 tcaatgcatt aaaagtaaaa aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt
     7981 ctcgtgtaac gaatatcaaa ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag
     8041 aatttcgtca agaaatcttg ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa
     8101 cgtacgaatt gactgatgaa gattggaaaa aaatcaatcg aatctctgag gaatattatc
     8161 gaaattggga ttggaattac gggaagtctc cagatttcaa tttctctcgc caaaaacgtt
     8221 tttctatcgg ttcgattgaa gtccatttga atgtctcaga aggaatgatc aaagaagcaa
     8281 aaatctttgg cgatttcttc ggactaggag atatctctga cgtggaaaaa aaattggttg
     8341 gacagaaata tgataaagca tctctatcaa aaatagtcag ccaaattgat gtgaagaaat
     8401 attttggtgc gatagaacca gaagaattgc ttaccttact ttattgaaaa taaaaaatag
     8461 cggaatccac atatatggat tccgctattt tttatacagg attcgggtaa tttcgagaga
     8521 gttgaacagt gatccgatca atgaaagtat ctttttcctg cgttagtgtt cctgtgtaac
     8581 gatccgctac gtctttcatc atcattaagt caaaacgttc ctgatcatct aattcttctg
     8641 tgatgcctcg actaaatagg tttgtatcgt aatgtgcact atgttcgcaa caaatgacga
     8701 aatgttcctc ttgttcgtgt atcgacaaat aaatttttcg atacagtgga ttttcgatgg
     8761 catgagtttc tctaaaggaa tgctctaaag cgtggatcaa aagttgagcc agatcgtcat
     8821 cgttgatagg aagttcttcg ggaagatctg cttggaaggt tatctgaata ttccgtttgg
     8881 cagctaactt ctggtaacgc gctaagataa gatttgtcac ctggtggttg caaaaagaac
     8941 acagcatttc gaaatttttt gcttcttcta atatcttttc cacatagttt ttagcttttg
     9001 tttggtccgt atgcattaaa tcttgcagat tctccatctc ttggacaaat tccaggcgaa
     9061 gtgtatttac ttcttggata tgctgtcgaa gctgctcata atgttgctcc aacaaatcat
     9121 ttttgatttt taagaaatct acttgtctcc gatctttgtc ggtatttaag atgtgatcga
     9181 ttagattgta gctgataata acgattagaa tgataatata taaaatcgta gaccaattga
     9241 tcgttgtttg atagatacct aatgccgttt ggatgtaaat caagcaatgg ataatggcta
     9301 ccaagacgat ccaaggtgta caagctacat aaaaacggtt attcttatat gcttcgccaa
     9361 tgcatgcaag tgaagtgact aaagtgctta taacactagc aatattagca atcggcatta
     9421 attcaggaag ttctgcagga gtaaaagttg cataaagcag aacgccgata aaaatcaaaa
     9481 gttggaacgt acaaaaaatt ccgtaccatt ttttatagta aaacattcgg ctccaatagt
     9541 agcagatgat gaaagctgac gttaagataa taaataaatg agataaaaaa gaatgaacga
     9601 atggttcaaa aagaatacca cttaaaatat cttctgatac agcttcaaat cctaatgcca
     9661 aagcaaagca acttaagaga ataagtgagt attcaagacg tttttgttta tacatcaagt
     9721 agatcgtata gaaaaaggtc cctatagcga taaaaacagc gattatcatc gtcaatactt
     9781 gcggcactga ttgtgaataa ataaaactaa tcaatggtcc agttgttcct agataaactt
     9841 tcggtggcag tccagcatag ttctgataag gagatgagac tgtaagggtc aatttttttc
     9901 cgatataatt tgctggtaaa tctatcacag ccaatgagag tccgggattg ctttcttcct
     9961 gataatcttt ccgttggtat aaaatctttt cgcctagata tacagtaatc cattgatggt
    10021 ttcctttgat tagtaaattt gcttcgtcca acggtttttc aagtgtcgtt tccataatga
    10081 atgtttcgtc tttatccacg gtaggaatcg tacgagtgta cttgctgtca aacttaaaat
    10141 caggatcatc ttttgtatag aaaacccacg aatcttttat ttctttcatt tgattgctgt
    10201 ccgcaggaga aaaaataaaa taaaagaaat aagcaattcc tgttaaaaat aacaatgata
    10261 atataatgat tatgctccac tttatacgta aataaaaaga ttctttttta ttatatatgt
    10321 tattaaaaaa agacataaag gtattccttt ctaaaacttg taatgttagg gtaaacgttt
    10381 tttaaactaa cgtcaattat ttataataaa aaatgagata aagatgagga aatagaatta
    10441 gaaacatata attattaaag ggaaacattt ttacagctat aatttgaaaa aaatttatcg
    10501 ttattatatg attaagaagg attgaataaa gaaagaagtt gaaaacgata cctgattaca
    10561 aaaaaataac ttttataagt tctataacgt tgattatatt tttaattctt attcttctcg
    10621 tcgtcttggt tccccgaatt tttggtttga ctccttatat tattgaggac aaagaaatga
    10681 gtccatatta tgcacaagga aatctgatat atgtacgacc gaaaaataca aatgagattt
    10741 tagtcggaga tattattaca tattatgaaa attcaggaaa aaaaatctcg acgcgtcgcg
    10801 taatagccgt tgaaaacaac cacgaagatt tttatacaaa agctgataca aagacttata
    10861 ttgagcctgg agtagtaaaa aagagaaata ttatcggtag tcccatcttt cagattccat
    10921 atattggact agtcctttca aaaacggcat ttgcatggat tcaggggtta ttctttacga
    10981 ttgctttttg cctaacagga atcacagcat ggaatacctt tgtagagttg aaaaagaagc
    11041 ggagacgaac acagaaattt ttgtaagaat gttaaaaaca taaatgtatt ccagagatcc
    11101 aacgactctt ctgaacatga aacacgagaa aacactcatg cagcttgtag atttagtcag
    11161 ttgttcgtat aatgtgtgaa gaggaggagt agtatgcgca atgaaatgat gcatcgtcct
    11221 gtagtcaaag aagaactatt ggattttatg cgtacccagc aaaaaaaatt accgggtgag
    11281 ctaggcaaac tggaagaaga agctcatgta gcagaagtac cagttattcc ccatgaaaca
    11341 gttgtatttt tgaaattttt acttggacag cttcagcctg aacgaatctt ggagatcgga
    11401 gcagctatcg gtttttcatc aagtgtcatg gccacgacaa tcggagaaaa cgctcatgtt
    11461 acgacaattg atcgtttcga tgtcatgatc gagaaagcta aaaaaaacta tgaacgtcta
    11521 ggactgacag ataaagtgac tttgcttgaa ggtcaagcag cagatatctt gccggagttg
    11581 tctggtccgt atgactttat ttttatggat agtgcaaaat caaaatatat tgaattttta
    11641 cctgaatgct tacgtttgtt aagaaaaggt ggcgttttga tggtagatga catcttccaa
    11701 ggaggaacga tcttgctccc agatgaagag attccccgtg gcaaacgagc tatccatcgt
    11761 aaattaaatg aatttcttcg tgttgtgatg gatcatcccg atttgacatc gacaatcttg
    11821 ccattaggtg atggtgtgat cttaatgaca aaggaatcag aaacaatcga gttgtgatac
    11881 atgattatgg cagcctgtag ataatcgatt gaatcgaggg ctcaattcga acttgatgta
    11941 aaagtttcta tatttgtaag tcaattaaag gatacaggaa atctaaataa acaggagtag
    12001 catgaagtca atgtagctcc tgttttcaat tgataacctt agaacagctg aaggagaatg
    12061 tctcgtttta aaagattgat ttagtagttg ggccaaaata attcattatt tgctaattaa
    12121 ataactattt cgtattgaca gaagcaattt aattatctat aattattgat gttccgctga
    12181 ttcttgtcat agtagctcag ctggatagag catccgcctt ctaagcggac ggtcgggggt
    12241 tcgaatccct cctgtgacgc taaaaaaccg ttagaaactg aggtttctaa cggtttttct
    12301 ttgttttttg tgccaatatt gtttgttttc aaaggcaaat aaaataaaaa aacatcctta
    12361 tcgagtacaa ttataaggag caaaaaaata aaactcatca gaaaagagga agaagatatg
    12421 acgatactat ctagaatttt agtgactcta gttgcaattg aattttttta tatcatgtat
    12481 atcgaaacgg tgagaactga ttctgacacg acaagtaggg tttttaaaat gagtaaagaa
    12541 gagttaagca gaaaatctgt tcaaacattg tttaagaacc aaggagtgta caatggacta
    12601 ttaggagtgg gactacttta tggagcttat ctttcttccg catctaaaga aatcacaagt
    12661 atgctattaa tatctatttt ttttgttgca ttatatggca gtttgactag cgatcgttgg
    12721 attttactca aacaaggtgg gctagctctg ttggcattgc tttctatttt cttataaata
    12781 tagaaaaaat aagaatttat ctaacaggaa actttgtgaa aaatattact ttatctcatg
    12841 aaatatgata ttctaatgaa tggtatttta gaaaatttca tgaaaaagga gttagatagg
    12901 tgttcaaaaa ttatgtttcc tcactaaaaa aagaattttc aggttatgat ctttcaagat
    12961 ttcagaaaga tcttttggct gggatcacag tagctgctgt agcattgcct ttagccttgg
    13021 cttttggggt atcaagcgga gctgatgcgg ccgcaggttt gattacggct atcattgccg
    13081 gtatcgttat tggcggttta tcaggcggtt tttatcaaat atcaggtccg acaggtgcga
    13141 tggcagctat cttgatgtct attgcggcaa caaagggaat gcaaggtgta ttgcttgcta
    13201 cttttttagc gggtctattt ttacttgcag caggactttt ccgattagga acactgactt
    13261 cttttatccc agcacccgta attacggggt ttacttcagg tattgctatc attattgcat
    13321 taggtcagat cgataattta tttggtgttc actcggaagg tacaaacgtt attgaaaaac
    13381 taagtagtta tcaaacgata ggatttccga taaacacagc ctctttgatc atgggagggc
    13441 tagtcatttt agggatgttt atctatccaa aaaaatgggc gaaacgaatg cctagttcgc
    13501 tgctggctat cattttggca acagcagtta tgctattgtt ccatttacca gtagcaactg
    13561 tgggtaagat tccacaaaca ttgatcagta gtaatcgttt gaatatggga gattttagct
    13621 tttctgcctt gcaacaagtg gcagttccag ctattagcat tgctttatta ggaatgattg
    13681 aaagcttgct atgtggtgct tcggctggcc gaatggcaaa taagccctta gatagcaatc
    13741 aagaattaat ggcgcaagga attggaaatc tgttgcttcc tttctttggc gggattcctg
    13801 ctactgcggc catcgcccgt acaagcgtgg ccatcaaatc tggtgcacaa actagattag
    13861 ctggtatgat acatgcagtt gtcttactgc tctccatgct tgtttttgcg ccagtgatgt
    13921 cacagattcc aatgccagca cttgcaggtg ttttgattgt gacggcttgg cgtatgaatg
    13981 aatgggaaac gatcaaggag ctatttacta ataaatattg gtcagcagtt ctattgttta
    14041 tccttacgat gatttgtaca gttatctttg atttaagtat tgctatcgtg atcggggtga
    14101 tcggcggctg tgtatttttt gttgtcaaaa gcgctgctat cacgatttca ttagaaacga
    14161 tcgattggca aagaatgggg ctgccagaaa cggaaaagct gaataactgg acagtagtgt
    14221 atattagtgg tccactgttt tttatgtctt cagaaaaact caaaaataaa ttggaagaac
    14281 taaatcatca cgatggtgtc attttctcca tgcgaggcgt accaagtatt gatttgacgg
    14341 cacgtgcagt ttttgatgaa tttcaagaaa aagcagagca aagaggacaa acgattatct
    14401 atacttcctt gcagccagaa gtagaaaaac aactttctca cttgtgggaa catacattca
    14461 cgcatcaaca tcaaacagtt gctcatgcgt tgacagcttt acatgcgaaa tatactgcaa
    14521 ttagccatta aaacaatgaa aaagaacaga caagactgta aactgaagat tttatggtct
    14581 tgtctgttct ttttaatatt gctgtaaaag tgaagctagt acttgccagt cttgatggat
    14641 aacaggtaca agggagcgat tgtagaaaat agctgctgga tgaaaaagtg gaagaattcg
    14701 atagtttttt tctgaccatt cgtaggcatc cttttgttct gttaattgta agacaggccc
    14761 tgtatagagt tgaccatgat ttttggtaac cgtatattct ttgccaagta aacgttgaag
    14821 accaatgttt ccaagtgtcg caattaattt tggctggacc ttcatcagtt cgtaatccaa
    14881 tattggtgca tgcgcaaata cttcggaacg agtaggtgtc cgatttggat aaacagtttc
    14941 tgactgttgt gtccgtttat tgatccgatg agtcacgcga tacgggcggc tccttacagc
    15001 actggtgata tacacctctt ctcgtgttaa gccagttgaa gaaagtgcct gcatcaattc
    15061 ttttccagct tgaccgctaa aaggaacatg cgtatcaatc tctgttttcc ctggggcttc
    15121 tcccacgagc atcagtttag gatgaagagg tccttgccca gcgataaatc cttctacagg
    15181 acggccgttc attcttaaag aagcttcttg tatcagttct tttgggtatt ccatttatgc
    15241 tcctcctttt tcttttatta taagaataaa agaaacacaa tgcgactatt ttatttggta
    15301 gaactgtgag gatagaaaag cagaagtatt gcttgaattt ttgttttact ttttgatttt
    15361 aaatttagtt gactaacggt tgctgttctg gtaagctagt actgttgtaa ttgtggactc
    15421 cacagctaca accgctcaga acaagtaata agcacagcat atgctgtcac ttcggatggc
    15481 gagtctaagt atatcagaag gaggtgcttt atagcatgta cgcaattatt aaaactggtg
    15541 gtaaacaagt aaaagttgaa gtgggacaag caatctatgt tgaaaaactt gacgttgaag
    15601 caggagaaaa agttgttttt gacgaagtta tcttagtagg cggagaatct acaaaagtag
    15661 gcgctccaac tgttgcaggt gcaactgttg aaggtactgt tgaaaaacac ggcaaacaaa
    15721 agaaagtcgt tactttcaaa tacaaaccta aaaaacattc acaccgtaaa caaggtcatc
    15781 gtcaaccata tacaaaagtg gttatcgacg cgatcaacgc ttaatagcgt ttgagaaaga
    15841 aggcctatat atgatcaaag gaacatttaa gcgaaatgac gcaggtcaga tcgtttcatt
    15901 cactttgacc gggcatgctg atgctggtcc ttatggaagt gatatcgttt gtgcgggtgt
    15961 ttctgcgtta gcgatcagca ctgtcaacgg gattgcctca ttagccggtt ttgagccgat
    16021 tgttgaaatg aatgaagaag aaggcggtta tctttatacc gaagtcactt caggaatgac
    16081 acaagaacaa aacaatattg ctgagattct attagaaaat cttttattag gattacaatc
    16141 gatagaagct gaaaactcag aatatatcca aattaaaaca attaatgata aataggaggt
    16201 gcagaccatg ttgttatcta tgaatctaca attatttgcc cacaaaaaag gtggcggttc
    16261 tacatctaac ggccgtgact cagaatcaaa acgtctaggt gctaaacgtg ctgatggaca
    16321 aacagttaca ggtggatcaa ttctttaccg tcaacgcggt acaaaaatct atccaggtgt
    16381 aaacgtgggt atcggtggag acgacacttt atttgcaaaa gttgatggcg tagtacgttt
    16441 cgaacgtaaa ggccgtaaca aaaaacaagt gtctgtttat ccagtagctc aagaagctta
    16501 attgaatctt atgattaaag aggttgtgac aagacttttg tcacagcctt ttttgtttat
    16561 aaaaataaaa gttgtagtgg aggctacgaa gtttactttg tctttttaca cttaatattt
    16621 ttttgccaat ttggaatcag gatgttttga taactatcgt tttcaattga tggaagctat
    16681 attgtttgct atcagtgaaa taattatatg gtaaacagtg ttctttttct tgtttccatt
    16741 ataaaaatcg tatcgggatt tttcgttctt ttaaattgaa catttctatc tatccgcgtg
    16801 ttatcaacaa aacaaaatca ggtaaatagt tgacgaaaca gaaataaata catagtatta
    16861 tgttttgaag cctaagtaca atagtctata gaaaaggagc gataccattg gcaaaacgca
    16921 ttgaccgaat ttatatgtat gtcaaagaaa aaaccgctca tttaacaaag gcagaatatg
    16981 atcaaggagt aacaacacag gaaattgctg aagtgttagg gattcagcga acaaatagca
    17041 gtaaagatct gaatcagcta gtgcgcgaag gaaaactgat aaagacagac ggacgaccag
    17101 tacgttacat atatcaaaca ctcgttacgc acgtaaagcc agttacaaaa catgtagtca
    17161 gttacaaaga aacgccggtc tctgaagaaa aaaatacact ccaaatagat actaaggata
    17221 tctttgcaaa aatcattgga gctaacggca gcatgaaaaa ttcagtggaa caggcaaaag
    17281 ctgcgatttt gtatccgcca agaggattaa attgtttgat tacaggacct accggatcag
    17341 gaaagactta ttttgcacac gcgatgtatc attttgcaaa agcgcatcat gtaatagatg
    17401 aggaaacgga actgattgtt ttcaattgtg ctgattatgc caataaccct gagcttttga
    17461 tgagtcattt atttgggtac gcaaaaggtg cttttacagg cgcagatgaa gaaaaaatgg
    17521 ggattatcga tcaagcggat ggcggtatgc tgtttttgga tgagattcac cggttgccgc
    17581 ctgaaggtca agaaatgatc ttttacttta tggatcatgg aacttacagt cgtcttgggg
    17641 aaacaacgaa atcccatgaa gcaaatgtac ggatcgtagg tgcgaccaca gaagacccag
    17701 gttcttctct attagaaact tttgttcggc gtatcccaat caatatcaaa cttccggctt
    17761 ttgaaaaacg tcctgcaaat gagaaagtcg atttagtaaa aatcatgatt gcccacgaag
    17821 caaaccgcac gcagcgaaag atttctttga cagaagatgt agcaaaagca ttgatcggca
    17881 gtgtgaccta tggaaatata ggacaattga agtcaaatat ccagttagta tgtgcacgag
    17941 gatttctaaa tcatatgcaa tcacccgaaa tctcgatcac aatcgatgat ttgacagaag
    18001 ggattcgtag cggattgatt caattagcaa gcaaccgcag tgcaatggca gagctttcta
    18061 aaatattgga gccaaagata actgtagcac caaatgatac gatcatgaaa atccaatcgg
    18121 actcctatga gttaccttat aatctatatg atatcatcgg agataaagca gctttactga
    18181 agtcggatgg tttggatcaa gaagcaatca atcatttcat ttctacagat atcaacatcc
    18241 atctgaagtc tttctataaa gaccatggtt tttcttttaa tgcagacagc aaattagcag
    18301 aatttgtaga tccgaaaatc atagaagtga ccaatcagat ttatgtaatg gtcaaaagat
    18361 ctttaccata tgagtttcag caaaacttta tttatgctat gagcttgcat atcagttctt
    18421 ttttgaaaaa aatcaactta ggagaagaac ggcatacgaa tgataacatc cgtgagatgg
    18481 cttttgattt tccagatgaa tatgctgttg cgaaagaagt gcgtagttat atcgaaacgt
    18541 attttcaagt ggagattcca gaaagtgaag actattactt gaccgttcta ctcgtctcgt
    18601 tacgggcaaa tcaagcaagc ggcagaatcg gtgtagtcgt tgcagctcac ggaaacagca
    18661 cagcaagcag tatggtccaa gttgtccaac agttgttaga tgcagagaac gtacgagcag
    18721 tagacatgcc tttggatatg gatccgaaaa cagcattgga ccgaattgaa cgcaatgtac
    18781 aagaagtaga tgaaggaagc ggggtcatac tcttagtaga tatggggtcc cttgcgtcgt
    18841 ttaattcgca aattcaaaga gacactggaa ttccagtacg tactgttgat atggtaacaa
    18901 cttctctagt tttggaaact gtacgaaagg tctcagtatt agggacagat ctagatatgt
    18961 tgcatgaatc attgaaaaat tttcgaggtt acgcagaaat atcttctgta gaatctgttc
    19021 aggaagcaaa acctattcat aagaaaaaag ctgttttggc tatctgcgca tctggcgaag
    19081 gtacagcaca aagaatcaaa gaattgatcg aacgagcggt aaacaagcgt caagaaacag
    19141 aattagaagt tctagctttg tcgattgtgg aagtgaaaga agaactgcca gttatccaaa
    19201 aagaatatca gatcatagct actactggga ttactgattt gaatatccaa gccccattta
    19261 ttcctttaga acgttttatt gatcaaaata tcgaagtcat tttggatcag ttgctgatgg
    19321 aatcagaatt ggaggaaact gaattcatct ccctagatga agagtcagct aaaaatacgt
    19381 gtgttgagtt tatctctgat aacttcatct ttattaatgg tagtaagtta atcgatccaa
    19441 tgtggcaatt ttctacacaa atctctcaga cgacgggaat cggcgatgaa gaatatggat
    19501 tcaaaatcaa cctagtgatg cacacagcag gaatgatcga acgtattatt cggaatgaac
    19561 cattgactgt ggaagaaaac gaattgacaa atacaacgaa tgatcctctt tatagccaat
    19621 tggcagcctc agttgttctg ttggaagatc agataaaagt aaaagtgccg atagaagaaa
    19681 tgtattatct gctacgtcta gtccataatc agttagataa aaaagaatac actgtgccat
    19741 gaatacacaa aaacgataca cttttatagt gtatcgtttt tgtgtattgg ttaagaatcc
    19801 tttaaattca attagtgtat cgtaaaaaag ttggcacgct ttttgcttat agtagtgtgt
    19861 gctaggaggt ggttgttcac acagaaatga taggggtttc tgtgaagtag tgaggaagaa
    19921 ggagttttgc agatggatat tcgattggca cgtatagatg atcgtttaat ccacgggcaa
    19981 gtcgcaacag tttggtcgaa gcaaacaggt gttgaacgta tcattgtgat cagcgatgta
    20041 gtcgctcaag ataaattgag aaagtttcta ctaaaagaag ctgcaccgcc ggggatcaca
    20101 gcaaatgtaa taaccgtgca gaaaatgatt caaatctatc atagtggatt attatcacga
    20161 gcaaaagtga tgcttttatt caccaatccc caagatgttg aagcggttgt ccgctgcggt
    20221 gtacagttga agtcgttgaa tatcggcggt atgagtttta ctgaaggcaa atcaatgatt
    20281 accaattttg tttcagtaaa tcaacaagat attgattcct ttcgttactt gaacagtcaa
    20341 gaagtggagt tagagattcg aaaagtacct gctgacagga aaatcctttt gatggaacta
    20401 ctagcgaaaa tcaaaaaggt gtagcaagaa tggttgttgg aaaaggtcaa ataattctgt
    20461 tgtaatggtt agcaattatt aatatttttg aatttgttac aacaaaagta aataggaggt
    20521 ataaatatgg tagggattat cctagcaagt catggacaat ttgctgaagg aatcttacaa
    20581 tccggttcaa tgatctttgg cgaacaagaa aacgtcaaag ccgtgatttt gaaaccaagt
    20641 gaaggtccag atgacttgag agccaaattg gaagaagcgg ttgcttcttt tgataatcaa
    20701 gacgaagtgt tattcttagt tgatttatgg ggcggaacac cgttcaatca atcaaacaca
    20761 ttattcgaag agcacaaaga taaatgggca atcgtcagcg gtttgaattt accaatgttg
    20821 attgaagcct acgcatcacg cttttcaatg gaatctgctc atgaaattgc agcacacatc
    20881 attgaaacag caaaagacgg tgtaaaagta aaaccggaag aattagaacc ggcagaagca
    20941 ccgaaagcag cagttgaaga cgcacaacct aaaggagcac ttccagaagg aactgtggtc
    21001 ggagacggaa aaatcaaata tgtattagca cgtgttgact cacgtctgtt acatggacaa
    21061 gttgcgacag cttggacaaa agcagtgcaa ccaaaccgta ttatcgttgt ttcagatgca
    21121 gtatcaaagg atgatctgcg taagagacta atcgaacaag ctgctcctcc aggtgtaaaa
    21181 gcaaatgtta ttcctattag caaaatgatc gaagtagcaa aagaccctcg ttttggaaat
    21241 accaaagcat tgcttttatt tgaaaatcct gaagacgtat tgacagctgt tgaaggtggc
    21301 gtagacatca aagaattaaa tgttgggtca atggcgcatt cggtcggtaa agtcgttgta
    21361 agtaaagtat tgtctatggg acaagaagac gttgaagcgt ttgagaagct tgaacaaaaa
    21421 ggtgtgaaat tcgatgtacg taaagtacct aatgattcac gcgataacat ggatgatatt
    21481 ttgaaaaaag caaaagctga attagcaaaa gcgtaataaa taatattaaa atttatagga
    21541 ggcttatcat gtctattatt tcaataattt tagtcgtatt agttgccttt ctagctggta
    21601 tggaaggtat tcttgatgaa tttcaatttc atcaaccact agtggcatgt acattgatcg
    21661 gtttagttac cggtaacctc gaagctggta tcgtacttgg cggtacgctt cagatgatcg
    21721 ctttaggttg ggcaaatatc ggtgccgctg tagcaccaga tgctgcatta gcatcagtag
    21781 catcagcaat tattttagtt ttaggtggac aaggtgttaa aggtgttcct tctgcgatcg
    21841 cgattgccgt tcctttggct gttgcagggt tgttcttaac aatgatcgta cgtacaatcg
    21901 ctgttccaat cgttcatttg atggatgcgg ctgctgaaga aggaaacatc cgtaaggttg
    21961 aaatgtggca catcattgct gtatgtttac aaggggttcg tatcgcggtc ccagcagcag
    22021 cgttgctatt tatcccagca caaactgtgc aatcattcct tgaatcaatg ccagcatggt
    22081 taacagatgg tatggctatc ggtgggggaa tggtcgtagc tgtaggttac gctttggtaa
    22141 tcaacatgat ggctacaaaa gaagtatggc cattcttcgt tattggtttc gttgtagctg
    22201 caatttctca attaacattg attgctctag gtgctttagg tgttgcatta gctcttattt
    22261 acttgaacct ttctaaaatg ggtggttcat caaataatgg aggcggaagc aactctggtg
    22321 accctttagg cgacattttg aatgactatt aatcttgaag gaggaaaata aaatggcaga
    22381 agaaaaaatc aaattatcaa aaaaagatcg tttagctgtt gcgtggcgct ctaccttcat
    22441 tcaaggttca tggaactacg aacgtatgca aaacggtggc tgggcatttt ctatgattcc
    22501 agcaatcaaa aaattataca aaacaaaaga agaacgttct gcagcattaa aacgtcactt
    22561 ggaattcttt aatacacacc catacattgc ttctccaatt ttaggggtta cattggctct
    22621 ggaagaagaa cgtgcgaatg gcgcgcctgt tgatgatgta gcgattcaag gggttaaagt
    22681 aggtatgatg ggtcctttag ctggtgttgg tgacccagtg ttctggttta cagtacgtcc
    22741 aatgctaggt gcactaggtg cttcattagc tatgggagga aatatccttg gaccaatcat
    22801 cttcttctta ggttggaaca ttattcgttg ggcattcatg tggtatacac aagaatttgg
    22861 ctacaaagct ggttctaaaa ttactgatga cctttctggc ggattgttac aagacgtaac
    22921 aaaaggtgct tcaattttag ggatgttcgt attagctgcc cttgtacaaa gatgggtatc
    22981 aatcaaattc ttaccagttg tatcaactgt aaaattagat aaaggtgctt acatcgaatg
    23041 ggataaattg ccttcaggtg gtgaaggaat caagagtgca tttgaacaag ttaataatgg
    23101 cttagccctt tctcctgaaa aggtaacaac attacaagac aacttagacc aattgattcc
    23161 tggtttagct gcattaggat taacattgtt atgtatgtgg ttgcttaaga aaaaggtttc
    23221 tccaatcgtt atcatccttg gtttattcgt agtcggtgtt gttggacacg tcatcggact
    23281 tttataatcc ataaaaatat agctcgggcg ttgtcccgag ctttttttat atagtatact
    23341 gttcataaac aaatatctgg agggagaaaa tggttcaatc aatcaataca aaagtagact
    23401 tggtaattga tgctacttcg tttacgggtc tgacagatta cgggcaaatc atgataggtg
    23461 ataatggttt tgagttttac catgcgcgag atagacgcaa atatatccaa attccatggg
    23521 aagaagtgga ttatgtacta gcatcagtca tgctaaaggg aaaatggatt cctcgatatg
    23581 ccattcaaac gaaaaaaaat ggtacataca cattttcttc caaagaacct aaaaaagtgc
    23641 ttcgtgcgat cagagaatat gtagatgaag atagaatggt acaatcattg agtttctttg
    23701 atgtagtgaa acgtggtatt cgtagtatgt ttaaacgcaa gtaaaaaata aagaaaactt
    23761 ctaagaggag gttttcttta tttttttact ttgcagaaga aaatatgaaa tagcaaaacc
    23821 aattatttgt cccctttaaa tcccatatgc taaaattgat tagcaaagaa ggaggttata
    23881 ttatgagttg gttatggaca ttgattgttg gagccgtgat cggagcaatt gcaggtgcga
    23941 tcaccagtaa aggaaattcg atgggctgtg taacgaatat cattgctgga ctagtcggtt
    24001 caagtattgg acaagcgatt ttcggcgaat ggggaccaaa agctgcaggt atggcactta
    24061 ttccatctat tttaggcgca gtgattttgg tagcagttgt ctcatttttc tttggaaaac
    24121 gatcataagt ttttacaaaa tagtaaacta aaaggagttg cagcgagact gtgactcctt
    24181 tttggcttta tttatt
//
LOCUS       DASOUD010000036        23407 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.036, whole genome shotgun sequence.
ACCESSION   DASOUD010000036 DASOUD010000000
VERSION     DASOUD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.036"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..1695
                     /locus_tag="U4A29_001314"
     CDS             34..1695
                     /locus_tag="U4A29_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO0680446.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1765..2112)
                     /locus_tag="U4A29_001315"
     CDS             complement(1765..2112)
                     /locus_tag="U4A29_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEO0680447.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2196..3137
                     /locus_tag="U4A29_001316"
     CDS             2196..3137
                     /locus_tag="U4A29_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680448.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3138..4181
                     /locus_tag="U4A29_001317"
     CDS             3138..4181
                     /locus_tag="U4A29_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680449.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4187..5257
                     /locus_tag="U4A29_001318"
     CDS             4187..5257
                     /locus_tag="U4A29_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680450.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5247..6200
                     /locus_tag="U4A29_001319"
     CDS             5247..6200
                     /locus_tag="U4A29_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0680451.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6212..7222
                     /locus_tag="U4A29_001320"
     CDS             6212..7222
                     /locus_tag="U4A29_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO0680452.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7507..8328)
                     /locus_tag="U4A29_001321"
     CDS             complement(7507..8328)
                     /locus_tag="U4A29_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO0680453.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8425..8703)
                     /locus_tag="U4A29_001322"
     CDS             complement(8425..8703)
                     /locus_tag="U4A29_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680454.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8971..10980
                     /gene="metG"
                     /locus_tag="U4A29_001323"
     CDS             8971..10980
                     /gene="metG"
                     /locus_tag="U4A29_001323"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO0680455.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11459..12046)
                     /locus_tag="U4A29_001324"
     CDS             complement(11459..12046)
                     /locus_tag="U4A29_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680456.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12221..13543
                     /locus_tag="U4A29_001325"
     CDS             12221..13543
                     /locus_tag="U4A29_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0680457.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13698..14474
                     /locus_tag="U4A29_001326"
     CDS             13698..14474
                     /locus_tag="U4A29_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO0680458.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14467..15036
                     /gene="rnmV"
                     /locus_tag="U4A29_001327"
     CDS             14467..15036
                     /gene="rnmV"
                     /locus_tag="U4A29_001327"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO0680459.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15029..15913
                     /gene="rsmA"
                     /locus_tag="U4A29_001328"
     CDS             15029..15913
                     /gene="rsmA"
                     /locus_tag="U4A29_001328"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO0680460.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16364..16993
                     /locus_tag="U4A29_001329"
     CDS             16364..16993
                     /locus_tag="U4A29_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680461.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17117..18226)
                     /gene="ugpC"
                     /locus_tag="U4A29_001330"
     CDS             complement(17117..18226)
                     /gene="ugpC"
                     /locus_tag="U4A29_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO0680462.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18435..18857
                     /gene="mgsA"
                     /locus_tag="U4A29_001331"
     CDS             18435..18857
                     /gene="mgsA"
                     /locus_tag="U4A29_001331"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEO0680463.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19180..19716
                     /locus_tag="U4A29_001332"
     CDS             19180..19716
                     /locus_tag="U4A29_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEO0680464.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19756..21519)
                     /locus_tag="U4A29_001333"
     CDS             complement(19756..21519)
                     /locus_tag="U4A29_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680465.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21522..23237)
                     /locus_tag="U4A29_001334"
     CDS             complement(21522..23237)
                     /locus_tag="U4A29_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680466.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7537 a         4288 c         4769 g         6813 t
ORIGIN      
        1 tattctgaaa attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg
       61 gttgcggtat gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt
      121 agttcagcaa aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa
      181 gaaatgccta cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat
      241 aacgtttatg aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct
      301 agcgagttgg cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac
      361 gcaaaatggt caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga
      421 acagtagacc cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca
      481 gaagacatta cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac
      541 tatgaattag aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc
      601 ccatcatttt tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct
      661 gcaagtgata aagctgttta taatggacca ttcacattag aagattttga tggaccagga
      721 acagatacag aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa
      781 ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa
      841 gatggccaag cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat
      901 ccagcttata cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa
      961 aagaattcac cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt
     1021 gaagctttag taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca
     1081 tcagatatgg taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta
     1141 gtttcttatg acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc
     1201 gttgattctt tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc
     1261 gaatatgttc aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct
     1321 gttccattct ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc
     1381 ggatgggctg ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat
     1441 tcatacaacc gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca
     1501 tcaacagacg ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc
     1561 atctctgatg atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat
     1621 gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca
     1681 tacgttgaag aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg
     1741 tcgcaacctc gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga
     1801 aagagcaatt agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga
     1861 cgagagcgag gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga
     1921 tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag
     1981 aaacggcaat gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt
     2041 gatgagattt tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga
     2101 tttttttttc atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac
     2161 atctgtcaga agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat
     2221 taaaacgtgt ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct
     2281 tgatgcaatt gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa
     2341 tcgagatgct aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt
     2401 atctttctaa tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag
     2461 tcgcacattt gttagctgga agaatcggac catccttgca gttagggtta caggcaatta
     2521 tctttggaac agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat
     2581 gggcagatac atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat
     2641 ttgctgtttt attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat
     2701 gggatggctt tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg
     2761 actctgctcg gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat
     2821 tagcaaaagc aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata
     2881 gtttgattcc attgatgact ttattaggac cgttagctgt agcattgatg accggatcac
     2941 tggtggtaga aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca
     3001 caacaaatga ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt
     3061 ttgttatttt gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag
     3121 aagggagccg aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc
     3181 ctgcggatga atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg
     3241 caccttcttt gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg
     3301 ttatttcaat ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt
     3361 ctcctcatga tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg
     3421 gaatcaatat cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat
     3481 atgctcaagc aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac
     3541 gtgatgtatt aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg
     3601 ctgcattact agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg
     3661 gacgtgtcga taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg
     3721 ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta
     3781 tggccatcac aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa
     3841 aagatcaaga atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat
     3901 tcaaacatat tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc
     3961 catctgctat tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt
     4021 cagcttcatt aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc
     4081 tattatggat tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg
     4141 atggattgcg tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt
     4201 agaagtgaaa gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg
     4261 cggtgtcaat tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag
     4321 tggaaaatca gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga
     4381 tgctggtcaa atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca
     4441 aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc
     4501 gacgatgccc attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa
     4561 aaaagaggga caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga
     4621 gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat
     4681 cgcgattgca ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct
     4741 tgatgtgact attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga
     4801 tacatcaatt atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt
     4861 cgctgtcatg tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa
     4921 cccgcaacat ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa
     4981 cgatcgattg tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga
     5041 cgcattctat ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt
     5101 ttttgaacta tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa
     5161 agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca
     5221 tacttacggt caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga
     5281 aacagtattt caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc
     5341 acatttatga aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta
     5401 ctggccgtac gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc
     5461 aagatataac gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga
     5521 tgatctttca agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg
     5581 ccgaaggaat tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg
     5641 atgatctatt gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat
     5701 tttcaggtgg gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt
     5761 ttatcatttg tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca
     5821 atttgttgca agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt
     5881 tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag
     5941 agatgggaac aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt
     6001 tatctgctat tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc
     6061 aacctgaacc agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg
     6121 tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat
     6181 ctcttctggt tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta
     6241 tccgacagaa gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta
     6301 taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc
     6361 gctcacaccc gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc
     6421 tttagcaaat gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc
     6481 cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca
     6541 aacccgtacg atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat
     6601 caagataaat cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg
     6661 atataatgaa gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc
     6721 tttagctaaa tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc
     6781 tctagaagta actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg
     6841 gaaattgaat gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag
     6901 caattatcag aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg
     6961 agaagattat cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt
     7021 agaatctttt ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt
     7081 aataacaaaa ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac
     7141 cgacggagca gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag
     7201 acattcggtt atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct
     7261 tgttaataaa aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc
     7321 actaattatg acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct
     7381 attgttaatt ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt
     7441 aactcacaaa gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc
     7501 acaaccttat tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc
     7561 tacaggacgt tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg
     7621 atcgatcgtt ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat
     7681 cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac
     7741 acggttgaat gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga
     7801 agactctcca gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt
     7861 gttactatga cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag
     7921 cggttcttct ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg
     7981 attaggcgta ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt
     8041 ttcttccttt gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc
     8101 accaggcaat gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt
     8161 cagctgatct tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt
     8221 gtcatattct ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt
     8281 atgttcataa tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc
     8341 attgacagta aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt
     8401 tacttgaaaa atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa
     8461 taaaaagaaa agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa
     8521 gccacaaata acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa
     8581 actgttcaaa tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa
     8641 tattaaatgt gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg
     8701 catgattgtt ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga
     8761 gaaataaaaa gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac
     8821 ctattcatcc ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt
     8881 cattttgaaa aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt
     8941 cgcatcatgc gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact
     9001 acgccaatct attatccaag cggcaaactt catatcggca actcgtatac tacgattgca
     9061 tgtgacgcaa ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga
     9121 gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa
     9181 gaatatgtag ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt
     9241 tatgataaat tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt
     9301 gatcgtttat tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt
     9361 tctgacgaag aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt
     9421 aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca
     9481 tacttcttcc gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc
     9541 gaattcatcc agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga
     9601 ttagaggatc ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat
     9661 ccgaaacatg tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga
     9721 tatggctccg aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc
     9781 ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat
     9841 ttgcctctac ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg
     9901 tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat
     9961 gcactccgtt attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca
    10021 gaagattttg tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat
    10081 cgaacgattg ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt
    10141 gtcactccat ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat
    10201 gatgcaatgg aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct
    10261 cgtgcaaaca aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag
    10321 aaagctgaat tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata
    10381 ttacttcagc caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac
    10441 tctgaaacaa tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta
    10501 gttgcaaaag gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt
    10561 caagaaaaaa tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa
    10621 gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta
    10681 gaactaaaag tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg
    10741 caattccgac tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa
    10801 ttttatcctg atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa
    10861 ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat
    10921 ggctctttac aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag
    10981 aaattcaaag ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag
    11041 caattttcgt aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa
    11101 ctatccaaaa taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga
    11161 ataatactaa taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga
    11221 ttttaattga ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc
    11281 aatgacttta ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt
    11341 tctatcactt tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa
    11401 aaaaacaatc atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc
    11461 acttgatttt atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa
    11521 gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt
    11581 gatagagctt tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta
    11641 taaaccaatg attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga
    11701 aataaaaata gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca
    11761 ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg
    11821 aatacaacat cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga
    11881 acaaagtcag caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa
    11941 ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt
    12001 tggataaata atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct
    12061 tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag
    12121 ggagtttttc actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac
    12181 tgaaaattat accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg
    12241 agaaatcaat ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt
    12301 cttcttcaaa actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc
    12361 ttggcttttg ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac
    12421 agcaatcccg gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc
    12481 cagcttttta ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt
    12541 atcgattatc tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt
    12601 gttgattgca gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt
    12661 cggtgcttct gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt
    12721 catatgggga ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg
    12781 aaaagatgtt acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta
    12841 tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt
    12901 gcctaaagca attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt
    12961 tgtttttttg aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga
    13021 agcatcagac gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat
    13081 ctcggtatac ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat
    13141 ggctttagaa gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac
    13201 ggttccttat gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg
    13261 ttctttcgac tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat
    13321 ttttatcggt gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt
    13381 accactttat ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat
    13441 gacgatgatg acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat
    13501 cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga
    13561 cagaaaaagt acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa
    13621 gttcttctat tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat
    13681 gaaaaggaga gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca
    13741 atgaagatat tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg
    13801 tcgtgggctt cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa
    13861 atatatacag tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg
    13921 aaaataaatt acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct
    13981 tggattatca ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga
    14041 ttgctatcgc aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag
    14101 acacgtatca aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt
    14161 tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta
    14221 gcggtgtcgt cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa
    14281 tcgaacgatt acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa
    14341 agcgaaatga accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc
    14401 ttccgacaga agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa
    14461 gcgaacatgg ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca
    14521 agaaggctgc gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat
    14581 gaagagatcc tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc
    14641 gatcctgatt tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca
    14701 aaacatgctt ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc
    14761 gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt
    14821 tcccaaacgg ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct
    14881 ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg
    14941 aacggcaaac aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc
    15001 caagcaatga aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc
    15061 tagaacaaaa gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa
    15121 ttttctgact gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca
    15181 tacgaacgtg atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca
    15241 tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac
    15301 gatgcaaccc tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc
    15361 aacagctgtt agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc
    15421 ttactatatt acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga
    15481 actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa
    15541 agcttatggt tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat
    15601 cgtaccaaaa acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac
    15661 aagaagagat ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa
    15721 agcggcattt cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa
    15781 agatgaccag acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa
    15841 acgtcgtggg gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga
    15901 aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag
    15961 taataagaac agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac
    16021 cgcagatgca agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc
    16081 taagtcacaa ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta
    16141 ttacttgatg caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt
    16201 gtcacatctc ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca
    16261 ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact
    16321 aagtcgacat ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt
    16381 tcgaaaacta acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat
    16441 gcctaaaatc atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat
    16501 tgcgatgttt tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt
    16561 ctttttaaca acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt
    16621 gattggggct tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac
    16681 ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc
    16741 agaaatgcta gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc
    16801 tcaaggatat ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt
    16861 agttgattat aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc
    16921 cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa
    16981 aacgacatat tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa
    17041 tcctctaaga tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc
    17101 tctaacaggg ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt
    17161 ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg
    17221 catctacttt tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc
    17281 caagtaattc ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg
    17341 ctacttgttc actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat
    17401 aacctttttc aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc
    17461 catctgtgat aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa
    17521 taaagccggc aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt
    17581 gttggataaa tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct
    17641 gatcgtgagt aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag
    17701 cacgcatggc tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga
    17761 cttttgcatc ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata
    17821 aagcagctgg ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca
    17881 cacgtttttt gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat
    17941 caaaaacagt catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt
    18001 ctttaggtgc cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat
    18061 cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa
    18121 aaacgataaa ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg
    18181 cattgtcata ttttttatat acgtttttca gagccatttc taccataata cgttccacac
    18241 tttctttttt agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat
    18301 gtcatgttga acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata
    18361 aatatccata agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa
    18421 aaggagtgtc tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg
    18481 tgaaattagc aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga
    18541 cgactgggca gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac
    18601 cgttaggcgg ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga
    18661 tttttcttcg agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc
    18721 gtttatctga tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac
    18781 taagaggact ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc
    18841 atccactttc attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat
    18901 gtagctataa aagctaaaca aactgataat atgcgatata atacgaacgt attggataat
    18961 ataaacaata atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct
    19021 gtgggaaaag atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga
    19081 tgggatgttg cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg
    19141 catgatacga tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac
    19201 gcacgtatga ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg
    19261 gtagcaatcg agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg
    19321 ggcattcttt ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga
    19381 atggccctct tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa
    19441 ggagcaatct acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg
    19501 gctacgctta gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg
    19561 atgtaccatg tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta
    19621 atatggttac cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa
    19681 aaaattttaa gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca
    19741 gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc
    19801 atctttttga agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac
    19861 taatatctgg tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt
    19921 tccttccatc attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga
    19981 gctggtagct tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt
    20041 caacagttgg cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa
    20101 tttttccggc aaacgttcta caaattcata catataagag gcttttaggg catcattgat
    20161 ttctgcgtca cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa
    20221 tagccaagta tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga
    20281 gcggatgtca tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag
    20341 caaattaaca agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc
    20401 tggataagca gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga
    20461 aacatgatca aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg
    20521 caaatcaggc ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa
    20581 agcggtttgc agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact
    20641 tgagtagatc gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc
    20701 caataggccg ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga
    20761 cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt
    20821 tagcagttca aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg
    20881 gttaccgatt cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc
    20941 gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga
    21001 acaaagaacg accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat
    21061 gatgattgcc actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt
    21121 gtccatatca tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg
    21181 aagtggcaat cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta
    21241 agctaaccga ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat
    21301 accaccgaat agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat
    21361 catttgatcg atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat
    21421 gattcctagt atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa
    21481 tcggcggagc gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat
    21541 gcgacgattt cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca
    21601 gcaatcttac cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt
    21661 tgggagataa taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag
    21721 tctgtttgat aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca
    21781 atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt
    21841 tccatgactt ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg
    21901 attttcagag cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt
    21961 tcacgaatag tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg
    22021 agatgatcca gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg
    22081 acatcataga aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca
    22141 atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca
    22201 gaatcaggag tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa
    22261 tcgggagtag tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc
    22321 acccgatttc ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc
    22381 agtgccaaaa gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag
    22441 ctaccaatat ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg
    22501 atcaaggttg tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc
    22561 gctagatcat ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg
    22621 cggatgacac gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt
    22681 tcttgcactt tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag
    22741 aaaataggca ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg
    22801 accactgacc cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa
    22861 gctagctgaa gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa
    22921 tgattcaact cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct
    22981 gtaccaaatc cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga
    23041 ccaatcacag acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc
    23101 atttttagtc cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca
    23161 gcttcaagaa atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa
    23221 cgtaataact gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa
    23281 gtactaccac attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa
    23341 tgaaagtttt attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag
    23401 aaaatga
//
LOCUS       DASOUD010000037        22772 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.037, whole genome shotgun sequence.
ACCESSION   DASOUD010000037 DASOUD010000000
VERSION     DASOUD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.037"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(68..1330)
                     /locus_tag="U4A29_001335"
     CDS             complement(68..1330)
                     /locus_tag="U4A29_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680467.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1323..2222)
                     /locus_tag="U4A29_001336"
     CDS             complement(1323..2222)
                     /locus_tag="U4A29_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680468.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2367..4145)
                     /gene="uvrC"
                     /locus_tag="U4A29_001337"
     CDS             complement(2367..4145)
                     /gene="uvrC"
                     /locus_tag="U4A29_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO0680469.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4327..4641)
                     /gene="trxA"
                     /locus_tag="U4A29_001338"
     CDS             complement(4327..4641)
                     /gene="trxA"
                     /locus_tag="U4A29_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO0680470.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4728..7088)
                     /locus_tag="U4A29_001339"
     CDS             complement(4728..7088)
                     /locus_tag="U4A29_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO0680471.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7203..7751)
                     /locus_tag="U4A29_001340"
     CDS             complement(7203..7751)
                     /locus_tag="U4A29_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO0680472.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7809..8246)
                     /gene="zapA"
                     /locus_tag="U4A29_001341"
     CDS             complement(7809..8246)
                     /gene="zapA"
                     /locus_tag="U4A29_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEO0680473.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8448..9365
                     /locus_tag="U4A29_001342"
     CDS             8448..9365
                     /locus_tag="U4A29_001342"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO0680474.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9621..10532)
                     /locus_tag="U4A29_001343"
     CDS             complement(9621..10532)
                     /locus_tag="U4A29_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO0680475.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10935..11513)
                     /locus_tag="U4A29_001344"
     CDS             complement(10935..11513)
                     /locus_tag="U4A29_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO0680476.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11533..12201)
                     /locus_tag="U4A29_001345"
     CDS             complement(11533..12201)
                     /locus_tag="U4A29_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680477.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12216..13286)
                     /locus_tag="U4A29_001346"
     CDS             complement(12216..13286)
                     /locus_tag="U4A29_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680478.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13457..14821)
                     /locus_tag="U4A29_001347"
     CDS             complement(13457..14821)
                     /locus_tag="U4A29_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO0680479.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14818..16377)
                     /locus_tag="U4A29_001348"
     CDS             complement(14818..16377)
                     /locus_tag="U4A29_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0680480.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16433..18913)
                     /locus_tag="U4A29_001349"
     CDS             complement(16433..18913)
                     /locus_tag="U4A29_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680481.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18897..19232)
                     /locus_tag="U4A29_001350"
     CDS             complement(18897..19232)
                     /locus_tag="U4A29_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680482.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19246..20280)
                     /locus_tag="U4A29_001351"
     CDS             complement(19246..20280)
                     /locus_tag="U4A29_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEO0680483.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20370..21050)
                     /locus_tag="U4A29_001352"
     CDS             complement(20370..21050)
                     /locus_tag="U4A29_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEO0680484.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21334..22437)
                     /locus_tag="U4A29_001353"
     CDS             complement(21334..22437)
                     /locus_tag="U4A29_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEO0680485.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         6503 a         4786 c         3870 g         7613 t
ORIGIN      
        1 ataggtctat acaaaaaaga agctgggaac aataaatgtc cagcttcctt ctatattttt
       61 ctatatttta tgcttttttt cgttcatttc tcataattga aatcgattgt ttcaatgaac
      121 tccataatcc gccttcgcta tacacgagta cattcgattt gtacatgttt gcagacaaga
      181 tcatcaataa gaatgttacg gctacaagta ccaacaagga aatcaatgct ccacccatac
      241 cgactgtctc atttgctaag cgaaccggca taatatatga agataagaat ggaatatacg
      301 aagtgatccg aataacgata ttgtttggat ctgaagctcc tagtatcaat ccaagcatat
      361 atccgcctag agatagataa gtcactggca aaattgcttt tgctgtatct tctgctttgt
      421 tgaccaaaga gccacataaa gctgctaaaa cagaaaaaat caaaatgcca aagatcatga
      481 atagaagaga ataccacaag aatggtccaa tcaagttatc caatgagata ccgtctaata
      541 tatttttgac catttccata tctttgaatt gactgtatcc tataccaaaa ataataccgt
      601 ataatgccat ttgcgttaat gcaacaagta aaacacctgt tagttttccg taaaaatgct
      661 tttgtgcagt cgtactggac aggatcactt ccatgatccg cgtgcctttt tcagaagcga
      721 tctcttgtgc aatgatctgc gcatacgtca aaataataat gaacaagaca atcgttgcta
      781 cataacttac gacatattgg actgtcgagt tatcttctcc aactgtcatt tttccttttg
      841 aatcaaaatt gactttttgt cgagtcaaac ttgcttgttg gctcaagcta gctacttctt
      901 ctggtgaaat gcctaattga cttgctcgaa gcatcgattg caagccagtc agttgttgtt
      961 gtatcagtag ttgtgtactt tgtcctaatg aattttcgct ataaagcatc ccatttatct
     1021 tatcattctc agttgagact actaaatacg catcgatttt ttcttccgaa agctcttttt
     1081 gtgctttttc ttcagaatca actgctttga actcaaaatc atctgttttg acttggctca
     1141 tttgctgact gatttgctca ttatcagcca atacgccgat tttattgaca tcactatttt
     1201 gttgagcaaa gtttcctgct acataaatga tacccagcat gataaaaggg actaatatca
     1261 tgatcaagaa agaaatcgac ttaacatttt ttaaatagac atctttggta ataatccaaa
     1321 acttacgcat cgatttcacc tgctttcaat ttgaaaatct cttccaaagt tggcggttgc
     1381 tggttaaaca taggaatgta gccgaactga gtcgcacgag taaagatttc tttcccagct
     1441 tcaggatctt ctaaggtaat ctctaacgta ttgtcttcat gaaggatcac ttttttcacg
     1501 ccttcgattt cttctacttc ctgttggctt aaaccagatt ccaagaataa cttcgtacgg
     1561 ccaaatgatt cacgaatctc atgtactttt ccattcaaaa ccatttttcc gtttcgcaac
     1621 atgatcaaat gatcgcagat tttttctaca ttatccatat tgtggctaga aaaaatgaca
     1681 catgaaccat gttttttcaa ttccaatatt ccatctttta gcaattcagc atttaccgga
     1741 tctaacccgc tgaatggctc atccaaaatg atcaatttag gttcatggat taacgtggcg
     1801 atcagctgga ctttttgctg atttccttta gacaaagatt ttactttatc cgtctttttc
     1861 ccttttacct gaaatttttc catccaaaaa tcgatttttg gttcaatctc ttttcttgat
     1921 ttccctctaa gttctgcaaa ataaagcagt tggtcttgaa tcgttacttt cggataaagt
     1981 ccccgttcct ccggaagata accgatgaca tcataatctt tctccccaag atgatggcca
     2041 ttccataaca cttctccttg gtcttgtgtc aaaaaatcca agatcaaacg aaaagtcgtt
     2101 gtcttcccag ccccgttttg gccaattagc cccatgattt ttccatcggg gatttggaaa
     2161 gagacatgat cgacagctgt gtagttccca aaagttttaa caagatcctt gacttctaac
     2221 atttcttcac tccttcttca agaaatcaca tatatcctta gacaccagac gtaagcgatt
     2281 tattatttac caacaaaacg taaacgctct ttttgttgta cataaaatga taggacactt
     2341 tactttcttt gtaaagtgcc ctttcctcat tcctgatgta atttatcaaa tacgttttgt
     2401 gcgacatttt tcggcagtcc gatttcttgt aattcttctg tcgtggctgc tgtgatattt
     2461 ttcaacgatt tgaactcttt aagcagcatt ttttttcgtt ttggtcctaa cccttcaatt
     2521 tcatctaatc gggaagcaaa actgttttta ctgcgtagct gccgatggaa agtgattgcg
     2581 aatcgatgga cttcatcttg gattcgctgg agtaagaaaa actctggtga atttcgctca
     2641 agcggtacta cagccaaatc tataccgaat agcaactcat ttgttttatg tttatcgttt
     2701 tttgccatgc cagcaatagg aatatcgatt cctaattgat tttctaagac atcttttgcc
     2761 acatctactt gtccttttcc tccatcgatc aagatcaaat caggtaaagg caacccttcg
     2821 cgaatcactc gtgaatatct gcgatagatg acttctctca ttgatgcata atcatccggt
     2881 ccgttgactg tttttatttt atatttccga tattcttttc ttgccggttt tccatcaatg
     2941 aatacgacca tcgcagccac aggattcgtc cccatgatat tagagttatc aaaagactcg
     3001 atacgtattg gggtcgggat attcatcgcg tttcctaaac gttcaactgc tccgatcgtc
     3061 cgttcctgtt ttctggcaat cagatcaaac cgttcattca aagcaactga ggcattctta
     3121 ttcgccaatt caactaattt tttcttttct cctcgttttg gttgcagcac tttcgtagct
     3181 aacaaagctt ctacactggg cttgtcgatc gtatcaggaa tcaagacttc tttaggaatg
     3241 aaatgctcat tttcctgata aaactgaccg aggtaagtca agaaatcttc ttctgcttcg
     3301 ttatagaaag gaaacattga aacatctcgt tcaattagct ttccttgtct tacgaaaaac
     3361 acttggacac acatccatcc tttgtcaatc gcataaccaa atacatctcg atccagtaaa
     3421 tcagtatttg tcattttttg gcgagtcatg attgtctcaa tggcacggat ctggtctcgg
     3481 tattctgcag ctttttcaaa ctccatgttt tcagcagctt tttccatttt ctgatggatc
     3541 tctgactcga ttttttcgtg tcctccgttt aaaaactttt tcacttcatc aacgattttc
     3601 gtatagactt tcggatctac gtcgaaataa taaggacaca gacattgtcc caaatgatag
     3661 taaagacaag gctccttcgt ctgactaggt ttgcattttc tcagaggaaa taatcgatcg
     3721 aggatcttct ttgtctcatt cgccgcaccg acatcgggat atggtccaaa atacagtgct
     3781 ttgtctttta gtactttacg agtaatcacc aaacggggat atttttcatt cgttatctta
     3841 aggaaaggat aggttttatc atcctttaac ataatgttgt atttaggatc atttttcttt
     3901 atcagattga tctctaagag caatgcttcg atattcgatt ctgtaacgat gtattcaaag
     3961 tcttcgattt cactaacaag ccgttccgtt ttcgtattat ggctgccagt aaaatatgag
     4021 cggacacgat tttttaatat tttggctttg ccgacataaa tgatcgttcc atttttatct
     4081 ttcatcaagt aacagccggg ctgatcagga agtaaagcta atttgttttt gattcgttcg
     4141 ttcatctggt actccccttc atattcaatt aaagttatta tagcatgtct gtcaaaaaaa
     4201 caatccagcc tgacaacgta attatactca aaaaatatta atttgaataa ccttaactca
     4261 accgcaaaaa aaccgaggac ggaactggat tcagtcccac cctcagctta tttttgctgt
     4321 aagtttttat aaatattgat cgatcatttg acgtagttgt tcttttgaat gaacaccaac
     4381 agctttttct actacttgac catctttttt caacattaat gttggaatgc tcatgatacc
     4441 gaattgttga ggagtttctg gattttcatc cacatccatt ttagcaattc tgaattcttc
     4501 ttcatcatat tcttgttcca actgatccaa aatcggtgct tgcatacgac atggaccaca
     4561 ccaagttgcc caaaaatcaa tcaatacaag tcctttatct gtttcttcat taaatgtctt
     4621 atctgtaatt acttgtgaca tatcaaagac ctccattttt aaaatctatg attagtatag
     4681 catttctata cttggatagc gaaccatctg cttaccttta gtaagcttta cttaaattta
     4741 acaattgtcg caccatttcc gccttggttt gccggtgcaa actcaaagct tttgacgctc
     4801 cgatggtttt taagataatc agtaattccc ttgcgaagtg cgcctgttcc ttttccgtgg
     4861 acaatagtca cttgaggata accagctaga atggcagaat ccaaatattg atctacctca
     4921 cttagcgctt cttcgtatcg ttttcctcga aggtccaatt gattcggcac gtgactacta
     4981 gattcagatc gtacagcatg gataactcgt tgtgtcggct ctttttgagg agcaacaggc
     5041 gtcagctcgt cttcggacac attcattttt aatataccga tttctacttg ccactgattc
     5101 ccgtttttac gtaaaagcgt ccctctttgg ccgtaagtcg taaccaacac ttcgtctccc
     5161 ggtttcaacg ttttttgttc tttcgccttc ttcaatactt tatttttctt caaagtttct
     5221 tcttgatgta gatttgctaa ttgggtcttt gcatcgatca actgatgctc tttcacattt
     5281 ccttgcccaa tctgctgttg cattttacga atatcagcaa tgattttttc tgctttttct
     5341 tccgcctctg acacgacttc gtttgctttt ttctttgctt tttccatttc tttttcacgt
     5401 tcttcaaaga aatagctgta agcttctttc aaatcatcgt gaagttcttg tgcctcagaa
     5461 acaaaatgac gcatttccag atattcggtc tctgccattt tccggcgatt ctctaaatct
     5521 gtgatcattt cgtttaaatc ttggctctca tcattcatca actgcttcgc ttcatcaata
     5581 acttctgtat caagacctaa tcggctagag atttcaaacg cattgcttcg tccaggaacg
     5641 ccaatcagca aacgatacgt tggacttagt gtatccacat cgaactccat gctggcatta
     5701 atcgtatttg cccgattgta tccataaact ttcaattcag gataatgcgt tgttgccatc
     5761 acataagcag actttttacc aagatcatct aagattgcga ttgctagagc agcaccttct
     5821 tgcggatctg ttcctgcacc gagctcgtca aataatacca gacttttttc attgactttt
     5881 gataaaatat ctacagtgtt cgtcatatga gaagaaaagg tcgatagact ttgctcaatc
     5941 gactgttcgt ctcctatatc cgcaaatact tcctcaaaaa taccgatttg actttcttca
     6001 cctgcaggaa tcggtaatcc tgcttgcccc atcagttgta acagacctaa agtcttcaac
     6061 gtgatcgttt tcccacctgt gttaggtcct gtaatgacga tggcttgata gtcttttccg
     6121 attgtgatgt cattagaaac tactttttcc tgatcgatca gtggatgtct tgcttgtttc
     6181 aagacgacat gattgtcttc actgattccc ggcacgatgg cttttacttc tttaccaaat
     6241 cgtgcttttg cattcatcag atccatttta ccaatcacat aagcattgtg gatgatttct
     6301 tgacgatggg gaacaagttc agcagaaagt tcacttaaaa tacgttgaat ctcatttcgt
     6361 tcagcgattt ggtattgacg caaacgatta ttcaaatcca ccacttgttt tggttcaata
     6421 aataaagtct gtcctgaagc actttgatcg tggactacac cgccaaatac accgcggtat
     6481 tcttgtttga ctgggataac gtagcggtca tttcgcatgg tgatgatcgc atcacttaaa
     6541 tactttgcat ttttcccacg gacgatccca tctaactgtt cacgaacagc ttgttcactt
     6601 cgccgaatgt tctgtcttat gctctttaac tcaggagaag cgtcatcagt cacgcgtccg
     6661 tcttcatcga ttgcttcctt taaacggcgg ctcaaaacag ggatcgtgac taactgatct
     6721 gcccacatat acaaacgtgc aaattcgatt tcactgtcag aaagatcatc aatgaaacga
     6781 ttcaattcgc ttgtcgtaga caatactctg gaaacttgtg caagctccac tccattcaag
     6841 tctgctccga tctcgatccg tttcatatgg ggacggatat tttcaagttt aggtataggg
     6901 atcccgcctc gcagacgttg cactttcaat ccatcctcag tttcttgaag ccagtttgtg
     6961 atcgtttgtt tctcactcac tggtaccaat tctgccagtt cttcttttcc ttgtgcagtc
     7021 actacaaatt gtctgaccat ctgtttgact ttttcaaatt ccaatgtttc aaggattcgg
     7081 ttattcattt tatcacctta tctttcacta tgaaaaaaga gctgtgccgg acatacgcta
     7141 cgcagccttc ttaaataaac tactctgtac aaaaaacgaa atattttcca gcactatttt
     7201 ccttaattca aaatattcgt tacccaaagc tcatacatct tgttggtaag aaatggcgtt
     7261 ctctcaacga taaaacgagc gaaactatgc tcgccgaatt gattttggac cacatctaat
     7321 ggaacaaagg caagtaactg caaaatcaaa aatatagtaa cgtaagccat gattagtgac
     7381 aggatgcctc ctgcaatcca gtctacctgt ctcaagatcg gcacatacgt caatccatgg
     7441 acaaaaaccc ccacaaatcg ggtaagcagc cagccaatta gcaaaataat cagaaaagca
     7501 gttcctgcat aaaaagcttc atctaggtga aaagcaaata cctgatcaaa aaatgctaac
     7561 tttgatgttg gggtgacagc tgggtatgga atgtataatt ccaaatgact tgctaatggt
     7621 ttgtaaaaat gctgggcagc aataaatgac aatacatatc caatcgcgaa tatgatttgc
     7681 aaagcaaacc cgcgacgtgc tccagaaaaa aacgcaatta gcaataaaaa taaaatcagc
     7741 aaactcagca tgctacgttc ctttctcttc taactttttt aaatttttct tattttgata
     7801 aaaggatctt aattagaaga tttttgttta atttgtcctt ttcgtttctc gtttaaaatc
     7861 tgttgtgctt ccacatgatt tttaatggta tgatctgctt gaccattttc tttcaatact
     7921 tcccgagctt cgttctcgat tgcttcaatg cgtttcacac gattttcaag ctctgtaaat
     7981 ttgatcattt ttttctttaa ttctgctgtt tcttcttcca gctccaagat tctttcttgt
     8041 ttcttcaatt gatccgaaag cgcattgaca gccatcaaga ttgctgcttg ttcattatcc
     8101 atttgtggtg ataattgttt caattctgct aactgttcat tgatcagctt cgttacgata
     8161 tccatatgat gcttggtttc ccgtccgata atagtatatg tttggttggc aatcactgct
     8221 ttgtagcgtg ttttttcgtg cgccatacga atcatttcct cctatttgat gaaagtttta
     8281 tttagtgtac catgaagttg gttctttcac cgttctccac aaatattttt cacatactta
     8341 actattatat gataaacttt aaaaaaatgc tatgtctcgc aatctcttag aaaacgaaaa
     8401 gtcgtgagtt gcggtatagt tttatgtata aaaacgagga gaattttatg agcaacgtca
     8461 ttatcaaagc aacaaacgac caacttagtc aaatgaaagc atactatcgc tcttatttgt
     8521 taaataaaca gattccttat actagctttg ctgcaaaaaa agacgggacg accattaccg
     8581 cttatacatc cggaaaagtg atgtttcaag gtacgaatgc agcaatagaa gctgataaat
     8641 ggggggaggc acaggataaa caagccaagc cagcaaaaac agcctcttct ttgccaaaaa
     8701 atatcagtca attatccgtt ttaggtagtg atgaggtcgg aaatggcagc tattttggtc
     8761 cgctaacagt atgtgctgct tatgtgaaac gtgatcagct tgcagaatta cgtaaacttg
     8821 gtgttagaga ttcaaaagag ttaaaagata gccaaatcat ccagcttgct gacatcttaa
     8881 aaaaaaccat cccctataaa ctgcttgtct tatctccaga aaaatacaat gagatccaac
     8941 caaaatacaa tgccgttcgg atgaaagtcg ctttgcacaa tcaagcgatc catttgctgc
     9001 taaaaaaaat cgagccagaa aaaccagaag cgattttgat cgatcagttt acgccggaag
     9061 caaattttaa aaaatatgcg cgtttagaaa aaaatcaatt acaagaaaaa ctgtacttcg
     9121 ttactaaagg agaacaatat catttagctg ttgctgctgc ttctatcatc agtcgtgcca
     9181 gctttttgga agagttacac aaagcatcag ctgaagctgg aatcaccctt ccatcaggag
     9241 ctggaacgaa atctgaccaa gtagcggcaa aactattgga gaaaggcggc atgcctatgc
     9301 ttgaaaaata tgccaaactt cattttgcca atacagaaaa agctttgaag ctgctaaaaa
     9361 aataatggaa ctgtgacaaa agtcatgtca cagtcccttg ttccgaataa acggtgttct
     9421 catgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc tgttttttga
     9481 aattgctcct tgtgacgcgg agttaaacgg caggctcaca acctcttcct taaacggtgt
     9541 tcaaaagctt gcatctgcga taataagccc ggccaaacga aaaatatgag gagctatttt
     9601 tgtttggctg ggcttattta ttactgaaaa taagtggtca atgcttgtgt caaacttgca
     9661 gcaatctgct gctgataact gtctgttact aattccttta aatcactatc attattcata
     9721 taacccatct cgattaaaat agaaggttga agattttctc gaagaacttg atagttccca
     9781 gtggagattc ctcttgaatc cagaggcaga tcagtgaagg agtcagcaat tgtctgtgct
     9841 aatgtttcct cttgtccata gtaataatat gtcgtgattc cagttgcttc attcgcatat
     9901 tccgtagagt ctgcgtgtaa actgataaag acatctgcct ttttttcttc actgtagtcg
     9961 catatatctc caagctgtac taattcatct gtagtatggg tcaaataaac cgttgctcca
    10021 gcatcctcta gcgccttttt tacttttatg gctgtactta atgttatatc tttttcttct
    10081 gtttgttcat cattgcttaa tgccccagta tcatttccgc catgcccagg atctaataca
    10141 atcactgcat cagatagctc ttccggtttt gtttgtatat tctttgtgac tttggttcca
    10201 tcttcgaggg ctaaatatcc agtatagcca tcatttgtct tgatctgata agcatttttt
    10261 gtttcagaaa gaaactccgc cagctctccg ccgtttatag ttgcaagtgg ggtagttagc
    10321 gagtcatctg tatatagtgt acggtcattt tttccgattt tgattaatgt ctcttgttct
    10381 gtatcatcaa atgattcctt tgtctttgta accgtactgt tcgtactgct gaccgtttgt
    10441 ccaactatct ttttttgacc aaaaacatct atttgaaaac agataataat gataatgatt
    10501 gcaaatacaa aagctccaat tatttttttc attcttgact ctcacctcgt agatccagac
    10561 taagggtcaa aacttaaaat cagcttaagt acgcaaaaaa ggggctgtaa aagatataac
    10621 cgtagaaaat aacattactg ctaaaatgat ttttttcatt tttggcatat cctgaaatct
    10681 ccttttgact attcgtggtg attcatctac ccaataaata gtttccttca ttctacaaac
    10741 aattaatttc ttcgttatta aattaaaaca cttcctggaa caccgacata ataaataaaa
    10801 atataccctg ataatcatga gagaataaca aatttgtatt tatgaaaaac tacctatata
    10861 aaaaaaacaa gatataacga tcttcgtcat atcttgcttc ttcacctaac tatttttctc
    10921 cctatataac agctttacgc ttctcctaat acaccattca aatataaatc gatcaattgc
    10981 gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg tgctaacact
    11041 aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac agattggttt
    11101 gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa gaaaagatga
    11161 tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa caaatcgacc
    11221 agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg
    11281 cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt ttctaaaact
    11341 gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggttg ggtgatccca
    11401 actttttgtg cgatctctct tgttgaagtg ccgagatatc ccttggacat aaacaagtca
    11461 gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac catttttcca
    11521 cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa atattctatc
    11581 gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat ttttgtcttt
    11641 tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca aactagcagt
    11701 tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga ttgcgaccct
    11761 ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat ccacgccaag
    11821 ctgctcaaat aattcttttg cttctttttg acgatgttca tgtttgatct tatctactaa
    11881 taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca aaataaagcc
    11941 aatttcttta aaacgtagtt taacgcgttc actttctttt tgattactga attcttgatc
    12001 attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt
    12061 acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat cgatactcat
    12121 ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt tttctacttc
    12181 tttaaattct attgctttca tgctcttcct ccttcttatc caattgcctt caacggatcg
    12241 atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac cagcatcaac
    12301 aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc aggtagaaca
    12361 agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga taaaatgaat
    12421 gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc tttcattaca
    12481 ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat cacaatagcc
    12541 gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc attatatcca
    12601 ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg taaattactg
    12661 attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg
    12721 aagcggatct cttggtaagc attgatggtt gtatagagaa ctggtgagac attgaatttg
    12781 gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa cttaactgta
    12841 tctcctacgg ctagaccata ttcttctttc aaactaatat cgcctactgc ctcatcatcg
    12901 ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt atcgataccg
    12961 aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac gactccaggt
    13021 gtctgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt cttagtaatc
    13081 atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc tgcctcccac
    13141 ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt caaaaaaaat
    13201 accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa ctttgaatgc
    13261 ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc attttataca
    13321 acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt cttccgctat
    13381 ttcattatat gaatttttta ttctcgattg attaatattt attatatccc cacacaataa
    13441 aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct ctcttagcta
    13501 gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtgttca gacagaaatc
    13561 caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata tccgcaagca
    13621 ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt atttctaatg
    13681 tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcatcg tattgtggaa
    13741 atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc gcttcatctt
    13801 ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt atcttttgtc
    13861 tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt gccatatgta
    13921 cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa ctgactggga
    13981 cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca ttgactatca
    14041 cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta ggacgagttt
    14101 gggcagaaaa aatgaccata tctgccttta ttgcttgaga tagaagttgt acggatacta
    14161 ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa ttgatttgct
    14221 tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat cttgataaaa
    14281 cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa gcatctgcta
    14341 attccaatcc aatataacca gcaccgaaga tcactacttc ttttgcttct tttaaagcgg
    14401 caagtacttg cttagattgc actagatttt tataggtgta gacattttca gcttcaattt
    14461 gtttatacaa ggtgacagga gaggaacctg ttgctacgat taatttatca taggttatgg
    14521 tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga tcaagttcag
    14581 ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc atgttatggg
    14641 caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca tttgaaacat
    14701 agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt
    14761 ttcttagatt tagggcagct gtgatgcctg catgggaagc accaatgatc acgattttca
    14821 tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa attggttcaa
    14881 ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag cggacttctg
    14941 tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt ctttctcgta
    15001 ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt ctttcacgat
    15061 atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta attctaactg
    15121 cttgatcagc gtccgcaaaa tagatgcata gcgagcaaat aattcaaagc gggcattttc
    15181 ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc tttcacataa
    15241 aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt cttggccaaa
    15301 aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc
    15361 aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt ctatcgattg
    15421 tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc ttatcaaaaa
    15481 ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa
    15541 aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa
    15601 tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa gattgctata
    15661 atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa gccatatttc
    15721 aaatttcaat cgtccttctt ctgtaatagt aaaactaaac tctttttgca gcagatctaa
    15781 aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagataag gacgtccaaa
    15841 ccaaaataat tggaaataaa aataacaaat ttgactttct ggaccggtca gccgcccgct
    15901 ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc tgcgagacaa
    15961 agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg agccattttc
    16021 caacagataa agaagtatct gatattttgt agaatagcgc aagtagcaat gataataaac
    16081 atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt ggataacaat
    16141 ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt ctttcaaggt
    16201 agcccgatca actgataagc gggtaagtac gttattaacg gaaatctctc ctttttcctc
    16261 taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca aactgaaaag
    16321 ttccaagtcc actcctccaa gcatttctga ttttaacagc agaagtttcc ttctcatcct
    16381 tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc acttagctgg
    16441 ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca ggttgagcat
    16501 tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca acgagaacct
    16561 ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt
    16621 gttttgatcc agaatgattg aagtataatt ttgttccgct taattcttga ccttgatcat
    16681 tcttaaataa agaagtcaat gtccctgtca gttgccaagg agacgtgctt ggaagccgag
    16741 tatcccgcac atcaaaagaa aaatcgcctt ttgcttcata ataacttgtc ttcaacggtt
    16801 tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt aattcttgtt
    16861 tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc ttagcaccat
    16921 ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg acggattgta
    16981 cagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta tctcctattt
    17041 tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct gcgatagttg
    17101 taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt gagatcgtga
    17161 tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa
    17221 tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg ctaaggtttt
    17281 ttagctgact atctgcactc acagatggaa aattagccac tgttatttga ggaagagtgg
    17341 tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg ttagtaagta
    17401 catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca ggtagtgtag
    17461 aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt tgatctgtcc
    17521 atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc gaaccctttt
    17581 ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa aggagctttc
    17641 cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct
    17701 ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc ccactgtaca
    17761 cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc agtattgctc
    17821 cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga ctaaatggca
    17881 actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg gtagagcctg
    17941 gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta ttatattttc
    18001 ctgttgaatc acttgttgag atgtaactat tccacgtatt tatctctgca cttgcagaaa
    18061 atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat ctacctgcag
    18121 ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa tcataaaaat
    18181 caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg gtcaaaattt
    18241 tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa
    18301 catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa
    18361 tatctgccaa tagagtattt gtatcccacg accccatccc aaaacgcaag gtatggcttc
    18421 ctgctgataa taaactaccg ctagtaacct tgatgggggc accggcaata tacaagtagg
    18481 cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc ggcaaattga
    18541 tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt acatttgtta
    18601 gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga tcatttgaac
    18661 catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt agttggacaa
    18721 gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt tcatacgtgt
    18781 atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga
    18841 taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat
    18901 tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa ccacgcaaga
    18961 agccataatt ctataccaat gatcaaataa agaaaacgga tcttttcttc attcgtctcc
    19021 gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg ctgttttgat
    19081 gcgccattga ctgaaccact tggatgcgga actggactta gatcataaaa tgtaatcgta
    19141 cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag caaacttgca
    19201 ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg ttgtttctcc
    19261 ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca ctagaactgc
    19321 agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa aaacggaatt
    19381 ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa atcgccattc
    19441 attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc
    19501 accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa attcattaga
    19561 cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata tcaaattgct
    19621 gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg cgttgacctc
    19681 gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac tttcttgtaa
    19741 aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg tagcattttc
    19801 tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc ccttaaagct
    19861 ttttgctggg attttcactt ctataggtat agttgtttct gcatgtgctg atacattgat
    19921 ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct catctaacgt
    19981 tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg gattgacaga
    20041 gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta gtgtctgatt
    20101 ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt ggttctctgg
    20161 aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga ttgacggaaa
    20221 aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat
    20281 tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta tagtattttc
    20341 accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac gaggttccaa
    20401 gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt gatatttgct
    20461 ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt aaatattggt
    20521 gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc agtggattcg
    20581 ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact
    20641 tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg tgcttggcca
    20701 tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt ttttgaagcg
    20761 tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg caattcagga
    20821 actacatcca atgttaaaga acctgtctgg ccagttgctg gattttccgg atctgctggc
    20881 tctactggtt ttgaaggatc agtagggtta agaggctcaa cggtatttgt tggttcagta
    20941 aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt tgccaagccg
    21001 cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat aataaatcac
    21061 tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc tcatgaattc
    21121 atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt
    21181 ttacatgaat attcttcctt ttttattaag tatgaaacaa tatttcaatt ttattttttc
    21241 gtttcaaatt attctattag tgacatcctt gaactataaa aataggagta cggcatcatc
    21301 tgccgcactc ctattttttt cgtcatttat ttcttaaata aaacgattct cacgcaattt
    21361 tatttttttg atatcttcta ctgtttgtgt tccagctaat tgcatgacca tttccaattc
    21421 tgttttaaag aaatcaaaca cttgatggac tcctgtactt cctcctaaag acaatccata
    21481 aataacgggt ctgccaatag caacaagatc ggcacctgat gcgattgctt tgaaaacatg
    21541 ttgtccacgt cgtacaccgc tgtcaaacac gattggtaca cgtttatcta ctgcttctgc
    21601 cacatattgc aacgaatcaa acgcagcagg ccccccatct aattgacggc caccatggtt
    21661 agatacccaa attccaccag caccagattc taatgatcga tagacatctt cttctgattg
    21721 aacacctttc acgtagactg gcaaatcaga atgagcagcg ataaattcta catcttttgg
    21781 actaagtttt tgtttggaag atttatatac agcatccatc gtttggccta ctcctgattg
    21841 ataagcctga acgatcggca ttggcagcgg gaaggtaaac ccgtttctgc ggtctgtttc
    21901 acggtttccg ccaactgttg catccgctgt caatacgatt gcttttgctc cattacgctt
    21961 ggccacttca agaatatcca aattgattcc attatctttg ctcatataaa attgaaacca
    22021 ttgcggtgct ttctccccgc ctgcttctct tatctcttct aaagtacaag aagcgtaaga
    22081 acttgctgta tagatcgtcc caaaatctgc tacacctttt gcagaagctt tctccgcctt
    22141 cacatgagct aatccatgag cagcaactgg tgccatgatg attggtgcag tcagcatttc
    22201 ttcgtcaaaa tgtgtagttg tatcgggtaa ttctacatct cttaatacat gaggaataat
    22261 caaacgatga ttgaatgccc gttcattttc ttgataagta aataagtcgc cagcaccact
    22321 gctgatgtaa ccataaccac cagctggaat cacttgtgaa gccgcgattt ctaaatcttc
    22381 catattgata aaatctacta ttccttcatt tgtacctgct tgatatgttt tttccatctc
    22441 aatgccttct ctctataaaa tttgaatgat gaacaatatg atagcgttta cactttttat
    22501 attactcttc gacattgata caggcaaata atctgtttac atttcagcca ttccagatgt
    22561 cactactctg gaagaaatga ttgtaataaa aatagataaa aaaccgatgg gactttgaca
    22621 aaagtagtgt caaagtccct ttttttgaat aaacggtgtt caaaagccat tttctcggta
    22681 ttaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt cttactactc
    22741 gaaactaagc gactttttca caacctctcg at
//
LOCUS       DASOUD010000038        22676 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.038, whole genome shotgun sequence.
ACCESSION   DASOUD010000038 DASOUD010000000
VERSION     DASOUD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.038"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            43..213
                     /locus_tag="U4A29_001354"
     CDS             43..213
                     /locus_tag="U4A29_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680486.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            433..1140
                     /locus_tag="U4A29_001355"
     CDS             433..1140
                     /locus_tag="U4A29_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO0680487.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1200..1844)
                     /locus_tag="U4A29_001356"
     CDS             complement(1200..1844)
                     /locus_tag="U4A29_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO0680488.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            1971..2258
                     /locus_tag="U4A29_001357"
     CDS             1971..2258
                     /locus_tag="U4A29_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680489.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2366..2686
                     /locus_tag="U4A29_001358"
     CDS             2366..2686
                     /locus_tag="U4A29_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0680490.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2767..4080
                     /locus_tag="U4A29_001359"
     CDS             2767..4080
                     /locus_tag="U4A29_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO0680491.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4077..4796
                     /locus_tag="U4A29_001360"
     CDS             4077..4796
                     /locus_tag="U4A29_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEO0680492.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4924..5475
                     /gene="raiA"
                     /locus_tag="U4A29_001361"
     CDS             4924..5475
                     /gene="raiA"
                     /locus_tag="U4A29_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO0680493.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5697..8231
                     /gene="secA"
                     /locus_tag="U4A29_001362"
     CDS             5697..8231
                     /gene="secA"
                     /locus_tag="U4A29_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO0680494.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            8358..9459
                     /gene="prfB"
                     /locus_tag="U4A29_001363"
     CDS             join(8358..8429,8431..9459)
                     /gene="prfB"
                     /locus_tag="U4A29_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO0680495.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9564..10250
                     /gene="ftsE"
                     /locus_tag="U4A29_001364"
     CDS             9564..10250
                     /gene="ftsE"
                     /locus_tag="U4A29_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO0680496.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10234..11127
                     /locus_tag="U4A29_001365"
     CDS             10234..11127
                     /locus_tag="U4A29_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680497.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11279..12727
                     /gene="cls"
                     /locus_tag="U4A29_001366"
     CDS             11279..12727
                     /gene="cls"
                     /locus_tag="U4A29_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO0680498.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            13064..13921
                     /locus_tag="U4A29_001367"
     CDS             13064..13921
                     /locus_tag="U4A29_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEO0680499.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14036..14956
                     /gene="pstC"
                     /locus_tag="U4A29_001368"
     CDS             14036..14956
                     /gene="pstC"
                     /locus_tag="U4A29_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO0680500.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            14956..15840
                     /gene="pstA"
                     /locus_tag="U4A29_001369"
     CDS             14956..15840
                     /gene="pstA"
                     /locus_tag="U4A29_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO0680501.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15855..16661
                     /gene="pstB"
                     /locus_tag="U4A29_001370"
     CDS             15855..16661
                     /gene="pstB"
                     /locus_tag="U4A29_001370"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO0680502.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16677..17435
                     /locus_tag="U4A29_001371"
     CDS             16677..17435
                     /locus_tag="U4A29_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO0680503.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17448..18125
                     /gene="phoU"
                     /locus_tag="U4A29_001372"
     CDS             17448..18125
                     /gene="phoU"
                     /locus_tag="U4A29_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO0680504.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18347..19381
                     /locus_tag="U4A29_001373"
     CDS             18347..19381
                     /locus_tag="U4A29_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEO0680505.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            19378..20145
                     /locus_tag="U4A29_001374"
     CDS             19378..20145
                     /locus_tag="U4A29_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEO0680506.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            20245..21813
                     /gene="liaX"
                     /locus_tag="U4A29_001375"
     CDS             20245..21813
                     /gene="liaX"
                     /locus_tag="U4A29_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEO0680507.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            21989..22297
                     /locus_tag="U4A29_001376"
     CDS             21989..22297
                     /locus_tag="U4A29_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO0680508.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            22297..22647
                     /locus_tag="U4A29_001377"
     CDS             22297..22647
                     /locus_tag="U4A29_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEO0680509.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         7466 a         3933 c         4910 g         6367 t
ORIGIN      
        1 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
       61 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      121 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      181 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      241 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      301 tctgacaata ggagtaggaa gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      361 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      421 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      481 aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      541 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      601 gaaggaatct tagaaagaaa gatcggttcg ggtacatatg ttgcgcgaga aaaagtacaa
      661 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcgcaaaa tcgagtaccg
      721 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      781 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      841 cctatttgtt ttgaagttgc cagtgtgcca caaaagctga tcgatggcta cagcaaagca
      901 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
      961 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1021 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1081 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1141 aaaacaggtg atccctgatt ccttacttag aaataaaaga gaatcagaat cacctgtttt
     1201 tatatatgat cagttaccag ttgctcgtga taagaaacag gaccctcttc aaattggact
     1261 tgtccgttca gcagctcaat gatggattct ttaaagtggt cagcactttt ttcatctaca
     1321 gcacagatga cgctaacttg atcagtatat tggatttctt ttataaaaaa atgttggagt
     1381 tcaagataat tctgtaattt tcctagttgc ggatacgtga tcatcacaga gatctcctgt
     1441 tgcaaggtac cttctacgat tccaatgtca gacaatgctt gagagacagc atgagtgtat
     1501 gctcgtatta atccgcctgt gccgagtttc gttcctccaa aatatcgagt gactactgca
     1561 gctacattga tcagttgatt ttttttgagg acttccaaca tcggtacgcc agccgttcca
     1621 cttggttctc catcatcgct gcttcgctgg atttcatttt tttctccaat gatgaaggcg
     1681 ctgcagttat ggttcgcttt ccaatgttct ttttttattt cttgaatgaa actttttgct
     1741 tggtcctctg attctattcg tctaagagaa cagataaagc gtgatttttt gatctcaatc
     1801 tcactttgtc cattttcttt tattgtacga tagcgctcga tcatcttgtc acctcttttt
     1861 ttcattttct ttattcagta taaataaagt tctaggttgt ttcaacactg taacggttgc
     1921 gcaatcgctg tagtatgcga taataaagac aaaggtaggt ggatctgggg atgcaggaac
     1981 gaatgaaaaa gtacgatgca attacgcatt atttgaagaa caatggcggc tctcaagtaa
     2041 cgttgacctt tacccaattt gacgaactgc tgtttcccag taacggactt cccaaaacag
     2101 caagggaaag tacagattgg tgggcaaatg attataaaca ccctgaaaaa ggtgcctatg
     2161 gctggataaa tgcaggctat gaagtagttg tcatcaattt ggacaaagaa tatgtcgtgt
     2221 tcaataaatt agtgaaatct agttggctat ttgattaaat aaaccgcgtt tagaaaacgt
     2281 tgacaaattt ctttcttttt atataataga gtaacagacg cgaagaaaac gtttgcgtag
     2341 tttgttttaa aaaggagaga aaagtatggc taaaaaaaca atcatgcttg tatgttcggc
     2401 tggtatgagc acaagtttac tagtaacaaa aatgcaaaaa gctgctgaag caaaaggaat
     2461 cgactcggat attttcgctg tgtccgcttc tgacgcagat aacaatcttg caaataaaga
     2521 tgtagatgta ctgttattag gacctcaagt tcgctttatg aaagctgatt ttgaaaaacg
     2581 tctggaacca aaaggcattc ctttagacgt catcaatatg gctgattatg gtatgatgaa
     2641 tggcgaaaaa gtgttagatc aagcaatcac gctcataggt gaataaatag aacagtataa
     2701 ctatcgaaca agcgatccaa aaaatagatc gcttgttttt tcgtatattt ttttagggcg
     2761 aataatatga atcatttttg gggtagaagg cttctgacga gagatattaa agcaatagac
     2821 tgtgaggagg taaatttacc aaattatgag aagatcgcag cagtggaacg tttgggcaac
     2881 tggattcttt gtcatcgctg tggaatgagg acgtccgtca ttgatggaca gcttgcagat
     2941 tacggttatt tttgtacaca ttgtctatat ttagggcgat gcgacactca acaagattta
     3001 tatttgtttg atcagcctga agcaaaacct cgagaagtcg tctttttttg gacaggacag
     3061 cttactgaaa aacaaacgga aatcgcaaag agaatacttt gccattcaga aaaaagacat
     3121 catcttattt gggctgtaac aggtgcaggg aaaacagaga tgctttatcc tattttagtg
     3181 gaaactttaa aagctggagg aagagtagcg atttgtacac cgcgagtcga tgtttgtaat
     3241 gaattgtttt tacgctatcg ttatgttttt ccagaagaaa aaatcacgtt gctccatggg
     3301 aatacagcaa cggcttatac ctttacttct tttgttatct gtacgattca tcagctatac
     3361 cgtttttatc ggtcctttga tttaatagtg atcgatgaga tcgatgcgtt tccttataac
     3421 ggggatgcag gattagctca tgctgtgcaa acagcgatta aaccagacgg acgattcatc
     3481 tacttaagtg ccacacctga taaaaagtta ctaaaggaaa tcaagcagac gtttgacatc
     3541 cacaaacttg ctttgcgctt tcatcggcgt ttacttcctg aacccgtctt ggttttcatg
     3601 aataattggc gacaaaaatg caagaacaag aagaagataa aatctttggt gcggatcatt
     3661 catcaattaa ttaaagaaaa ccatatcctt ttattttgtc caagtatcac gatgatgcat
     3721 cgtttgaaag ccacacttca agaggctctt ccgagatatg agatcacaga tgtttcttca
     3781 agagacaatc agcgggcgga aaaagtccag aaaatgcgag aagcaaaata tgacatactg
     3841 ttaaccacga tgatccttga acgtggagtc acatttgaaa gaatatccgt tatcgtttta
     3901 ggagcggatc atccggtttt tactaaatct tctttggtac aaatcgcagg aagagccgat
     3961 cgtaaagggc cttatacgaa cagtcaagta tactttttct atgaagaaaa aactgcagct
     4021 attcgaaaag cttgtcagga aatcaaagaa atgaatgaag aagggaagaa gatgctgtga
     4081 ggtgcagttg ctgcagtaaa gatatcgtcc aaaatttaac attcagcgag attattggtg
     4141 tttcgaaata cagattacct cagacttgtt cagtttgtcg cgcacaattt catttattgg
     4201 aaacaagagg ctgttctggc tgtcaaaaac caatccattt caatctagag agtacgaaaa
     4261 agaaagtaaa gtctattccc gtttactgca gtgactgtca agaatggaag aagaaatatc
     4321 cgacctatga ttttcagcac cgtgcctttt ttgtgtacga tgaaaatatg aaggcctggc
     4381 tcaatcaata caagtttatg ggagatatcc gactagcagg cacctttgca tataaatgga
     4441 cgcagataaa aaaagaatac ccgacataca tctattgtcc aataccttta tctgaaaaaa
     4501 ggcgaacaga aagaggattc aatcaagtaa gcgagatgtt gaaagcagca agaattcctt
     4561 ttcagatgct actcaaaaga gagaagcatc tgctggctca agcaaaaaac acgcgaaagg
     4621 aaagattgga gatgcctcaa ccttttgaac ttgcagtcac cttttctaag atacaaaata
     4681 aaaaaatatt gattattgat gatgtctata caacaggaca gactcttttc cacgcagcag
     4741 attgtttgca ggtcgcacag ccgaaaatca tccgaaccat ttctttagca agatgaatta
     4801 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
     4861 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
     4921 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
     4981 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
     5041 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
     5101 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
     5161 ttggttgttg acaaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
     5221 tcccgtgaaa cgggcatgaa tactgcagat gctactgctt tattcaatga agaggacgaa
     5281 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
     5341 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
     5401 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
     5461 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
     5521 agctgccgca agtttttttg agtttcctcc tcttttagat gaaatatgaa taaattatga
     5581 agttcttact ttaatacgct taagatattt caattatcta taactaatga taaaatgaaa
     5641 agtaaagtct acatagtctg gataatacac agaaggaaaa ggagattaca caacgcatgg
     5701 ccaatttgat tcgttcactt atcgaaaacg ataagaaaga actaaaacgt ttagatagca
     5761 ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc attaactgat gaacaattac
     5821 aggcaaaaac tgctgaattc aagggcagat accaaaaagg agaaacatta gatcaattac
     5881 ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg tgtattgggg ttatatccat
     5941 accacgtaca attaatgggt gggatcgtgc ttcacgatgg gaatatccct gaaatgcgta
     6001 ctggtgaagg taaaaccttg actgcaacga tgccggtgta tctgaatgcg ttgactggcg
     6061 aaggggtcca cgtagttacc gtcaatgaat atttagcaac ccgtgactcg acggaaatgg
     6121 gcgagcttta taatttctta ggattgacag ttgggttgaa tatcaactca aaatcttcgg
     6181 atgaaaaacg cgaggcttat aattgcgata tcacttatag cacgaataat gaattaggat
     6241 ttgactattt gagagacaac atggtcgttt accgtcatca aatggtacaa cgtccgctga
     6301 attatgccat tgtcgatgaa gttgactcga tcttgattga tgaagcgaga acgccgctga
     6361 tcatttcagg gcaagcagaa aaatcaacag ctttatatac aagagcagac aacttcgtga
     6421 aacgtttgaa agaagaagaa gattataaaa tcgatataca atcaaaaaca atcagtttaa
     6481 ctgaagcagg gatcgaaaaa gctgaagaaa cattcggttt ggacaactta tatgacattg
     6541 aaaatactgc attgacccat cacttggatc aagcattgag agccaactac atcatgcttt
     6601 tagacatcga ttatgttgtt caagatggaa aagtattgat tgttgaccaa tttacgggtc
     6661 ggatcatgga tggacgccgt tattcagatg gtcttcatca agcgatcgaa gctaaagaag
     6721 gcgtagagat cgaagacgaa acgaaaacga tggcgacgat cactttccag aactatttcc
     6781 gtatgtacaa gaaacttgcg gggatgactg gtacagctaa gacagaagaa gaagagttca
     6841 gagagattta taatattcaa gtataccaga ttccgacaaa ccgtccggtc atccgtgacg
     6901 atcgaccaga tttgctttac ccaacattac aaagcaagtt ccatgctgtc gtacaagata
     6961 tcaaagatcg ctaccgcaaa ggacagcctg tcttagtcgg aactgttgca gttgaaacat
     7021 ctgagttatt atcagatatg ctgaatcaag aacgtattcc tcatgaagta ctgaatgcga
     7081 aaaatcactt taaagaagca gaaatcatca tgaatgcggg tcaaaaaggt gcagtgacga
     7141 ttgctaccaa catggctggt cggggaacag atatcaagct agggcttggc gtaagagaac
     7201 ttggtggcct agcagttatc gggacagaac gacatgaatc tcgtcgtatc gataaccagc
     7261 ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc acaattctac ttgtctttag
     7321 aagatgattt gatgaaacga ttcggttcag aacgaatcaa agctttctta gatcgaatga
     7381 agatcggtga cgaagatgct gtgatccaaa gcaagatgct gacgaaacaa gtagaagctg
     7441 cacaaaaacg cgtagaagga aacaactacg acacacgtaa gaacgtcttg caatacgatg
     7501 acgtgatgcg tgagcaacgt gaagtaatct ataaacagcg tcaagaagtg atcatggaag
     7561 aaaaaagctt atctaaacca ctgatgaata tggtcaaacg gacaatcagt cgtgtggtag
     7621 atgcgcatac gcaattagaa aaagaaaatt ggaatttgga aagtatcgtc gattttgctg
     7681 gcagcacgct tgtccatgaa gacagtattt cccttacaga tatcgaagga aaaacctctc
     7741 aagaaatcaa agaatatcta aacgaacgag cacaagaagt attcaatacc aaagcagctc
     7801 aattgaacgg accagagcaa ctgcttgaat ttgaaaaagt cgtgatctta cgtgtcgtgg
     7861 actctaaatg gacagatcat attgatgcaa tggaccaatt acgtcagtca atcggcttgc
     7921 gtgcctatgg tcaaaacaat ccattagtcg aataccaaac agaaggttat aagatgttcg
     7981 aagatatggt aggtgccatt gaatatgaag tcactcgtat cttcatgaaa gctgagatcc
     8041 gtcaaaacgt acaacgtgag caagtttctc aaggaacagc ttctcactca gaagatggag
     8101 atgccaaaga aaacagtaat actagcgcga aaaaacaacc aatccatgtg aacaaggttg
     8161 gtagaaatga tccttgtcct tgtggaagcg ggaaaaaata taagaactgt cacggaaaag
     8221 atgcaaacta atttctgaca gcaggattgg tgagacaaat tcttttttaa agaatttgtc
     8281 tcaccttctt tctgttattt gacgattctg ttagactaga tataataaaa aaagtatagt
     8341 gaaagaagga aaaaaacatg gaaatatccg aaattcgaaa tctcctgaca gaaatgcagg
     8401 aaaaggtcac aagcttcagg aggtctcttt gacttagacc agctggaaga agacattgcg
     8461 gaagcagaag caaaaatggc tgaacctggt ttttgggatg atagcgaagc tgctcaaaaa
     8521 gtgatcagtg aaaacaatgc aaataaagaa acttatgatc aattccattc tcttgctgaa
     8581 gaactggaag aaatcgaatt gatggtggag atgttgcagg aagaacctga tgcagtaatg
     8641 caagaagaag cagaagcacg tatcaatgcg ctagatgaaa aaatgaatac gtatgaatta
     8701 tctatgttgt tagatggtcc ttatgatcga aacaacgcca tcgtcgaatt gcatccaggg
     8761 gctggcggaa cggagtccca agactggggc agcatgcttt tacgcatgta tacacgctgg
     8821 gcagagcagc atgggtatca agtagagaca ctagattatc aagcaggcga tgaagcagga
     8881 atcaagagtg tgaccctact gatcaaagga cacaacgcat ttggctatct gaaatcagaa
     8941 aaaggtgttc atcgccttgt acgtatctcg ccttttgact cagcaaaacg tcgtcatact
     9001 tctttttgtt cggtagatgt gatgccagag cttgatgaaa cgatcgatat agaaatcaat
     9061 ccagatgatt tgaagatcga cacatatcgg gcgagtggtg caggtggtca gcacatcaat
     9121 aagacagaat ccgctgtacg aatcactcac ttgcctacag gaacagtagt agcgagtcag
     9181 gcacaacgtt cacaattgaa aaaccgggaa caagcgatgg ggatgctgaa agctaagctt
     9241 taccaattag aagtggaaaa gaaagaacaa gaagcagctg cactgagagg agaacaaaaa
     9301 gaaatcggct ggggatcaca gatccgttct tatgttttcc atccatattc tatggtaaaa
     9361 gaccatcgga caaacgtaga gacaggaaat gtccaagctg ttatggacgg agatcttgat
     9421 ttatttattg acggctattt gaaaatgcat ctgcaataag agccctttgt taaattgaaa
     9481 taaaattgta agattttgca agcaagttga aacattgcca tgctataata gctaaagact
     9541 gaaaaagata tggagagaac gccatgattg aaatgaagga tgtaatgaag aagtactcaa
     9601 acggtaccac tgctatccgc aatctctcga ttgtcatcga acaaggagag tttgtgtatg
     9661 ttgttggtcc gtctggagct ggtaaatcta cattcatcaa attgatgtat cgagaagaaa
     9721 aagcttcaaa aggaactttg aacgtcgcag gtcatgatct aatagccatc aaaaaccgcg
     9781 aagttcctta tctacgcaga gaaatcggcg tggtgttcca agactataaa ttattaccaa
     9841 gaaaaacagt atatgaaaat gttgcgtacg ccatgcaagt tatcggcaga aaacctcggg
     9901 atatcaaaaa acgagtaatg gaagtgctgg atcttgttgg tttgaaacac aaagtccgag
     9961 tctttcctag cgaattatct ggtggggaac aacaacgtgt ttctattgcc cgagcaatcg
    10021 taaatacacc taaagtactt atcgcggatg aaccgacagg gaatctagat ccggaaaact
    10081 catgggaaat catgaaacta ttagatcgga tcaatgcaca aggaacaact gtcgtgatgg
    10141 ctacacataa cagtacgatc gtaaatacga ttcgacaccg agtaattgct atcgaaaacg
    10201 gccgcattat tcgagaccaa gcggaagggg aatatggata cgatgattag aacctttttc
    10261 cgccatttat tagaaagtat caagagtttg aaacgtaacg gctggatgac agtagcttcg
    10321 gtcagtgcgg taacgatcac gttgacttta gttggtgtgt tcatggcggt catcatgaac
    10381 gcaaccaaac tagcacaaga tatagaagga aacgtggatg tatctgtgtt cgtagacatt
    10441 ggtacaaaag acgaagagat gaaaaaatta gaaaaagaat taaatgactt agatcatgtc
    10501 aaaaaagtcg aattttcaag caaagaacag gaattgaaga aaatccaaaa cgaaatgggc
    10561 gattcttgga aattgtttga aggggataat aacccgttat atgatgtata cgtagtaagt
    10621 gctaccgatc cttcttatac aaaacaagta gcagaaaaag cggcagattt gaagaatgtt
    10681 tcccgttctg attacggtgg ggcttcctca gatcggattt tccaaattgc tggtgcagtt
    10741 cgaacatggg gattagttgc ggcggcattg ctgctgtttg tagcaatgtt tctcatttct
    10801 aacacgattc ggatcacgat tttatctaga cagcgtgaaa ttcaaatcat gcgtttagta
    10861 ggggcaaaaa atggatatat ccgctggcca tttttccttg aaggtggttg gatcggctta
    10921 atcggtgcca ttcttccgat actgatcatt acatttggtt acgcatggtt ttaccaattg
    10981 atcaatccgt cattattacg atctcattat tcattgatcc atccagaaaa tatcgtttgg
    11041 aaaatcaatc ttttgatggt cggtattggt gtgatcattg gttcactcgg ctcgatcatc
    11101 tctatgagac gtttcttgaa attctagtgt cacaagataa atataatcag aaagttggac
    11161 gatttcgttc cagctttctt tttttatcag ctaggtggag taaatggggc ggcttttttc
    11221 tctttacttt aaaaaacaac ttgggtattc ttaagttggt agaaaaagag gtgaagccgt
    11281 gaagattatc gttgacaatt tttttacgat ccttttgatt atgaatattt tgctttcctt
    11341 tattatcgtt tttcgagaaa gaaaggaaac tgcgcaaaca tgggcatggc tgctcgtttt
    11401 gatgttcatt ccagttgtag gatttatttt atatattttt ttaggaagag ggatttccaa
    11461 agagaaaatc tttgatttga agatccaagg gaaaatcggt aagaatctag aaatcgaaga
    11521 agataaacaa gcattgatgc gagggcttta tcctcatccg cctactggac aagtagatgt
    11581 gaaacaattg atctatatgc ttactgtatt tgaaagtacg ctttatacga ctaaaaatga
    11641 aatcacttta tttacagatg gtcgtgaaaa attcgatgcg ctgatcgagg atatcaaaca
    11701 ggcagaagac catatccatt tccaatatta tatttaccgc agtgatacat taggtgaaga
    11761 agttcgagag gcattgacag aggcagcaag acgcggagta aaagtcagag tccttcttga
    11821 tgcatggggt tccacacaag tttcttctag tttttttcag aatttgaaaa aagcaggtgg
    11881 agaagtcgca tttttctttc ctctattcgt tccgtatatc aatcctcgaa tcaattatcg
    11941 caatcatcgt aaaatcgtag tgattgatgg gaaaatcgga tatacaggtg ggttcaatgt
    12001 tggaaacgaa tatctaggat tggtaaagaa atttggttat tggcgagaca atcatctgcg
    12061 tatctatgga gaagcggttt acaccttgca aaaccgtttt ttgatggact ggaattctca
    12121 gcatacaaaa gaagtaggat attcaccgga atttttcccg accattcatt ctacaggtga
    12181 tatggctgtt caaatcgtga caagcggacc agataccgaa catgagcaaa tcaaaatgac
    12241 atatttgaaa atgatttcgt tagccaaacg ggagatattg atccaaacgc cgtattacat
    12301 accggatgga tcgattcatg aggcattgaa gttggctctt ctctcaggcg taaaagtcca
    12361 tatccaaata cctaacaaac cagatcatct tctcgtttac tgggcaacgt attctttcgc
    12421 tgcagagctg atcgagtatg gggcaagaat cgaaacatat gaaaatggat ttatccatgc
    12481 aaagacgatg attatcgatg gcggcatcgt ttccgttggt tcagctaata tcgacgttcg
    12541 ctcatttaga ttggattttg aagtcaatac actgatctat gatgaacgaa tggcttctcg
    12601 cgtaagaaaa gcattctttg aagattcaaa gatttcgacg catctcacaa aagaagtgta
    12661 tgaaaaccga gggatgatga tcaagatgaa agaaggatta gcaagactga tttcgccgct
    12721 cctttaaaat acaaaaagga caggagagcc atcaatagat ggaaatctcg cgtccttttt
    12781 tctcggggcc atttctgaga gtacgtcaga aatggagaaa gcgtctcgca tgagcgaaat
    12841 taactttggg gtatctcttg ttttttgggg aaagcaagaa ataagctaat atatgctatt
    12901 aaaagagaca catttcaaag ccagtatagc atagaaactt ttaatgagat agcttttttt
    12961 gcaattatga gaatttattt tgttatttaa tgattttgat tcgttttcat caagtaatga
    13021 aaatgataga ataaacatac tttaggatta aaggagattt ctaatgaaaa aactaaaatt
    13081 attcttacca ataataggat tagctattct tttatcaggc tgttcaaagt ggattgatcg
    13141 aggagaatcc attactgcag ttggctcttc tgccttacag cctttagtag aaacggttgc
    13201 tgaaacctac caggcagaaa atcctggaaa attcatcaat gtacaagggg gcggaagcgg
    13261 aactgggttg tctcaagtac agtcaggagc agtcgatatc ggaaattctg atttgttcgc
    13321 ggaagaaaaa tcagggattg atgcttcggc attagtcgat catcgagtag cagttgttgg
    13381 aatcactcct attgtgaaca aggatattgg cgtaaaagat atcagtatgg aaaacctgaa
    13441 aaaattattt acgggcgaga tcaaaaactg gagtgaagtc ggtgggaaag atcagccagt
    13501 agttatattg aaccgtgcat caggaagcgg gacccgtagt acgtttgaaa aatgggtatt
    13561 ggacggagaa acagctgttc aggcacaaga acaagattca agcggaatgg tgcgccaaat
    13621 cgttgcggac acacccggag caatcagcta tgttgctttt tcttacgttt ccgatgatgt
    13681 ggatacgttg aacgttaacg gtgtggaacc aacagatgaa aatgtgacaa cgaacaaatg
    13741 gacgatttgg gcatatgagc atatgtacac aagagaccat ccaaaaaaac tgacaaaaga
    13801 atttttagac tatatcttat ctgatgaagt acaagataat attgtcggag agctaggcta
    13861 tatccctgtt tcacatatga aagttgaacg tgattggcaa ggaaacattg ttaaagagta
    13921 atctttacac aaaatttaca aacttagcta aacttttaca tgttattaaa accaaattga
    13981 tgttcaattt gtacactgag tgagtatgtt aaacctgatt aagggggagt catctgtgga
    14041 agatgtgaaa aaagtattat taacaaaatc aaaacgttca aaaatggaac agcgcggaaa
    14101 attcattagt tttttgtgta tcgcgctgat cgtgttggtt gtattatcta ttttttattt
    14161 tgtcgctagt aaaggattgg ccaccttttt tactgataag atcaatgtat ttgattttct
    14221 gtttggtaca gattggaatc caagccaaat aggagcaaat gggaaaccca tggtaggggc
    14281 attgcctatg atcacaggtt catttattgt gaccttttta tctgctattg ttgccacacc
    14341 atttgctatt ggagcagcag tgttcatgac ggagatttct cctaaaaacg gaacgaagat
    14401 cttgcaaccg gtcatcgagc tacttgtagg tattccgtct gtcgtttacg gatttatcgg
    14461 tttatctgtt atcgtgcctt ttgtcagaag tatctttggc gggacagggt tcggtatctt
    14521 agctggtaca tttgtgttat tcgttatgat cttaccgact gtcacaacga tgactgtgga
    14581 tgcattaaag gcagtgccgc gacactatcg ggaagcttca ttagcgctag gtgccacaag
    14641 atggcaaaca atctacaaag tggtgctgcg agcagcagtg cccggaattt taacagcagt
    14701 cgtttttggt atggctcgtg cgtttggcga agccttagct atccaaatgg ttattggtaa
    14761 tgcggcatta atgccaacag gacttactac tcctgcttct acattgacgt ccatcttgac
    14821 aatgggaatc ggaaatacaa tcatgggaac agttgaaaat aatgtgttat ggtcattagc
    14881 gctgatcttg ctattgatgt cattattctt taatatcgtg atccgtatga ttgggaagaa
    14941 aggagagctg aaataatgaa cgcaaaacgc tcagataaaa ttgctacagg tgttctatat
    15001 gtggtttcag ggatcatcgt attgatctta gcagctttat tactttatat cttagttcga
    15061 ggaataccgc acatttcatg gtcattcttg actgaacctt ccaaagccta ccaagaaggc
    15121 ggtgggatcg ggattcaatt atttaactcg atttatttgc ttttgattac catgatcatc
    15181 agttttccta tctctttagg tgcgggtatc tacctttcgg aatatgccgg aaaaaactgg
    15241 ctgacagacg tgatccgtac gtctatcgaa atcttgagct ctttgccttc tgttgttgtc
    15301 ggtttattcg gtttcttggt tttcgttatt caattccagt atggtttctc aatcatttct
    15361 ggtgcattag ctttgaccgt attcaacttg cctcttttga ctcgaacggt cgaggaatct
    15421 ttacaagcgg tccattacac acaacgagaa gctggattgg cgttaggttt gtcacgctgg
    15481 gaaacagtga taaaagtggt tgttccagaa gcgacaccag gaattttgac tggtgtgatc
    15541 ctaagttcag gaagaatttt tggtgaagct gccgcattga tttatacagc tggtcaaagc
    15601 gcgccagcat tagattttac gaactggaat ccattaagtg tttctagtcc gatcagcatt
    15661 ttccgccaag ctgaaacact ggctgtccat atttggaaaa tcaacacaga aggtacgatg
    15721 cctgatggtg tttctgtttc ggctggcgct tctgccgtgt tgatcattgc tgttttactg
    15781 ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc gaaaaatgac ttcagcctaa
    15841 ggagaagaag taaaatgaaa aaatataatt ggaatgaaac aaatgtcatt accatgccaa
    15901 aagataagga agttgcatta tatacgaatg atctgcatgt ttggtatggc gacaatgaag
    15961 caatcaaggg agtggatcta gaattcgaaa aaaataagat cactgcattg attggtccct
    16021 ctggctgcgg aaaatccacg tatcttcgtt ctttgaatcg tatgaatgat gggattgcta
    16081 atacgaaaat tactggaaag attatgtata aagacgtgga tatcaatgcg cctcaagtgg
    16141 atgtttatga aatgcgtaaa cggatcggca tggtgttcca acgccccaac ccttttagca
    16201 aatcgattta tgacaatata acttttgctt tgaaacagca tggtgaaaaa aacaaaaaga
    16261 aattagatga aatcgttgaa acaagcttga aacaagcagc tttgtgggat caagtaaaag
    16321 atagcttgga taaaagtgcc ttggctttat ctggcggaca acagcaacgt ttgtgtatcg
    16381 ctcgtgcaat cgcgatgaaa cccgatatct tgctgttaga cgagccggca agtgccttgg
    16441 atcctatttc gacaggaacg gttgaagaaa cacttgtaaa cttgaaagaa gactacacga
    16501 tcattatcgt gacgcataat atgcaacaag cagctcgtat cagcgactat actgcctttt
    16561 tctatctagg aaaagtaatg gaatatgaca aaacaaacaa gatatttacc agaccaaaaa
    16621 ttcaggcaac cgaagattat gtgtccggtc attttggtta agacggaggc ctcaacatga
    16681 caaaagaaat tatctcatcc aaagatcttc atctgtatta tggaaagaaa gaagcactaa
    16741 agggaattga tctaagtttc aatcaaggtg aaatcacagc gatgatcggt ccttctggtt
    16801 gcgggaaatc tacctatcta cgttcgttga atcgaatgaa cgatctgatt ccaaatgtaa
    16861 cgatcaccgg aagtgtttta tataaagaca aagacatcta tggtccgaaa acagatgttg
    16921 ttgaactaag aaaagaaatc ggcatggtgt tccaacagcc aaatccattt cctttttcta
    16981 tttatgaaaa tgtcatctat ggattgaagt taaaaggtga aaaagataaa aaagtccttg
    17041 atcaagtagt ggaagaaagt ctgaaggcag cgtccgtttg ggaggatgtg aaagataaac
    17101 tgcataaaag tgccttgtct ttatccggtg ggcagcagca gcgtgtatgt atcgcacgtg
    17161 ttttggcagt gaatcccgaa atcatcttat tggacgaacc aacaagtgca cttgaccctg
    17221 tttctactgg gaaaatcgaa agtatgctgt tagaattaag agagcggtat acgatgatca
    17281 tcgtgaccca taatatgtct caagcttcac gtatttcaga taagacggca ttctttttag
    17341 atggtcactt gatcgaatac aacgatacga agaagatatt catgaatcca gaaaaacaag
    17401 agacagaaga ttatatttca ggtcgatttg gataaggagg aaacggaatg ctacgtacac
    17461 aatttgaaga agaattgctg aatctccata atcagttcta cgaaatgggg atgatggtga
    17521 gcagcgctgt gcacaagtca gttcgtgcat acatcaaaca cgataaaatc ttagctcaag
    17581 aagttatcga aaacgatata aatatcaata atatggaaac gcgtttggag aagaaaagtt
    17641 ttgaaatgat tgcattacaa cagccagtta caactgactt gcgtatgatt atcacggtca
    17701 tgaaagctag ctctgattta gaacgtatgg ccgatcatgc ggtgtccgtt gctaaatcca
    17761 caatccgcgt aaaaggacag acgagaatac cggaaatcga aaaagagatc tccgatatgt
    17821 ccgattacgt gaaaaaaatg gtggacaacg tcctagttgc gtatgtcaaa acagacgaaa
    17881 aagatgcgcg tacaatcgcc aatatggatc aaagagtcaa tgactatttc aatcgaatct
    17941 ataatgcaac catcaaaaac atgcaagaaa atcctgaaac agtcatcagt ggcacagact
    18001 atttgaatgt tgccagctat ctagaacgaa tcggagacta tgtgacaaac atctgtgaat
    18061 ggatcgtcta cttggctaca gggaaaatca ctgaattaaa tacgaaccac aatgagattt
    18121 tctaaactga agttgtgatt ctgctgttta tttaggaaaa gaggttgtga aatcagcgtt
    18181 ttgtcctgag tattagagaa acaaacggta aaatagttcc tcatattttg ccgttttttg
    18241 agctaatacc gaaggtcagc tgattgaaca ccgtttattc tggaaaaggg tttgtgacaa
    18301 aacatgtcac aaaccctttt cattattcca atagaaagga taaagaatgc aaaaaaagat
    18361 tagcatcatc atcactctat tttttatttg tatacttgct tcatggttta ttcagcgtca
    18421 agctgctcta tatgacgtac ctgtcatcga gatcgaaaga gtgagaatag aagaggatca
    18481 acaattgatt acaggaagat tgatgaatac ggaccaaaaa ggagaagtga tttctttaac
    18541 ggctccttat caaaaaaatc aaatcgaaac agcaaatata acaacaagac aaatctatct
    18601 tgtccaaatg catggagatg aatggcaact agtagcaaaa aaaaatgatg gctggctttt
    18661 tttctttaca agtgtcttca ttggcaccat gttgcttatt ggtggaaaac agggcgtatt
    18721 tgctttagga agtctcatca tcaacatcgt tttactttta cttttgttgc tcctctttac
    18781 caaaacagaa ggtatttctc tcttaaatat agcaatctta tttgtcgtta tagggataat
    18841 cgttagtata ttggcgttag acggatggaa tagaagcagt ataatcaaaa tttcggctgc
    18901 agttacaagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
    18961 tcaaggactg cgctatgaag aactgaatta tctgacacga ccgtatcgtt ctgtttttct
    19021 ttccagcgtg ctgattggga cagctggagg gacactcgat accgtgatca cagtcattgc
    19081 gacattagaa gaattgacca agcaacaacc gaaaatatca gcgaaacagt tatggctttc
    19141 aggaaggcaa gtgggccgag atgtgattgg ttcgatggct aatatccttt tattcgtcta
    19201 tatcagcgga gcgattccat cgcttctgtt atatttagga aatcaatggt ctttcaaaga
    19261 aacggtagaa cgacatttat cattagaact tttacgcgtc attgctggca gcttaggaat
    19321 agtattatca atccccatcg cggtcctctt ttttctcgct gtaaggaggt taaaaaaatg
    19381 aatgtactta ttatacttgt tgggattttt gcaatcagtg tgctatttgt cggtggtacg
    19441 caaggtatgt atattctttt aggattattc attaatttag gaatcttttt cctattatta
    19501 ttcggctatc atcagaagtg gccgatattg gtattgtcga ttatagggtt cctattgatc
    19561 gctgtggtta tcttattttt tatcaatggt tataatctga aaatgcgagc cgcttttgcc
    19621 agtatactga tctttttgtt ttgtttccta ctattgattc ctatcactga ttttttagcg
    19681 atccaaggct ttacttcgat agaactagaa gaactatctg gcttagataa gacgttagca
    19741 attgatttta gactacttac gaggtcgttg cttctgatca gtcttagcgg agctgtttta
    19801 gatgcgagtg tagcgatttc tagtggaaca ttcgaagtat atcaggcaaa tccgcaaatg
    19861 tcttttaacc aattgcgcca tgctagcttt gccattgcta aaaaaatatt ggcatcaaca
    19921 gtgatgacgc ttctttttgc gtttatgggg agttctttag cgttgattct ttggtttatt
    19981 gacttagaca tttcttttca gcaaataatc aatgaaaaat catttgtctt ggaatatacg
    20041 atggctattc ttaccacact ggccgcacta cttgtcctgc ccttaacttg cgtgactaca
    20101 gcatatctct ttacaaaaaa gaaagaacat ctcaagatgg agtaaaagtt acaacttaag
    20161 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
    20221 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga cttagtgaaa
    20281 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
    20341 gacgaaaaac aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
    20401 caggcaagtc aattgatcga tgaacttgaa aacggtgaag aaaaaatttc tgagccagtt
    20461 gatccaaaag aaaaagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
    20521 gcaactgaag cgaataaaac ttcggcgcgt ctagacgaag taaatgctca attcgctgac
    20581 aataaggcag cacgtaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
    20641 ttaggcgagt tgacagaaga agaattagca cagcgtcaaa cgttagaagc agaaatcaaa
    20701 gagttagaag caaccggcga tacactatta gaagaacaat tgaagctgga agcagaatta
    20761 aaagatatcc gcaagaatca atggtctgag aaaaaagaaa catttacgga taagtttgaa
    20821 ttgccagatg attggaaaga tcaagcgaca gatacgctga atcaagtagg cgaaaaaatg
    20881 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
    20941 acagtaaatg ataacatgga atggaaagat gtcagcttac gcgtccctgg aattgcgaca
    21001 acgaaatttg aacacgaatt ttattatgaa gctcctgcag caagtatcct tgatatcaag
    21061 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagtg atgatgtgaa agtggaagcc
    21121 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
    21181 cagatcgagg tcaatgaaga tcatatctcc ttccaaattc caaataagcg tgtgcgtgca
    21241 gaccttgttt tctacttgcc taaacgagta tacgaccatg cagctatcaa acttctaaat
    21301 ggcaacatca tgattgagac attggaagct aaagatattt atacaaaatc tacgaatggg
    21361 aacatcatcg tgaaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
    21421 atcgatattc gaaacgggaa catcttagat tctattatag aaacagtaaa cggtacagtg
    21481 acatttggtg ccacaccaga aaacctttct gtgtctttag taaatggtga tgtacgtctt
    21541 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg gaatgtcaaa
    21601 gttgctttac cagatagcat cggtttggaa ggacacgcta aaacaagtct tggaagcatt
    21661 aacagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
    21721 ttgcaattcc gtcgtgtttc agatgatgag atcgcacaag tgcagctttc aacgacaaca
    21781 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
    21841 tgagcaataa gaagcgattt tttaaaatag cttcccctat tttaaaaaaa tgtgccttat
    21901 tgccgatagg ctatctcttg aacaccgatt aagaagaagg ttgtcacaac gattttcttc
    21961 atttaaaata gtagaacaga ggtgagacat gatgaaaaaa aagttgacaa aatccaataa
    22021 aaatgttgtt ttgacaggta ctttagcagg gattggggaa tatatcggga ttgatcccac
    22081 tgttatccgt gtgctttatg tattcttaag cttagttgct tttggaagtc caatcatttt
    22141 atacatttta ttagctttga ttatcccatc tgcgcgttct gcacgaacaa actatggaca
    22201 taacaatgca tattatgaaa aaaacaatta tcgtgaacaa acaaaaaaac gcaaagaagc
    22261 ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca gcgtattttg
    22321 gttaatacgt taacattcat atcgttggct gttctttttc ctgcgatggt ccatgtgagc
    22381 agtatttgga cggccgtcct tgccagcttc gtattatctg tgttgaacat gtttgtcaaa
    22441 ccgattttga cgatcctgtc cctgcccttt acattagtca cattgggatt gtttacgttt
    22501 gttatcaatg cagcgattct taaaatgact tccttttttg taggagcaga acattttagt
    22561 ttttcttcat ttggtgcagc gttattcgta gctatcttga tgtcacttgt caatgtaatt
    22621 gttagtgaac ataacagcag aaaataaaaa aagctgtgcc gagatctcgg cgcagc
//
LOCUS       DASOUD010000039        22662 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.039, whole genome shotgun sequence.
ACCESSION   DASOUD010000039 DASOUD010000000
VERSION     DASOUD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.039"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(400..2781)
                     /locus_tag="U4A29_001378"
     CDS             complement(400..2781)
                     /locus_tag="U4A29_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEO0680510.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEETGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2771..3418)
                     /gene="recU"
                     /locus_tag="U4A29_001379"
     CDS             complement(2771..3418)
                     /gene="recU"
                     /locus_tag="U4A29_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO0680511.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3482..4021
                     /locus_tag="U4A29_001380"
     CDS             3482..4021
                     /locus_tag="U4A29_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO0680512.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEVRLISMDDLQNFSE"
     gene            4111..4530
                     /gene="gpsB"
                     /locus_tag="U4A29_001381"
     CDS             4111..4530
                     /gene="gpsB"
                     /locus_tag="U4A29_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO0680513.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4552..4919
                     /gene="rnpB"
                     /locus_tag="U4A29_001382"
     ncRNA           4552..4919
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U4A29_001382"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5128..6294
                     /locus_tag="U4A29_001383"
     CDS             5128..6294
                     /locus_tag="U4A29_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO0680514.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6340..7836
                     /locus_tag="U4A29_001384"
     CDS             6340..7836
                     /locus_tag="U4A29_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEO0680515.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7917..8600
                     /locus_tag="U4A29_001385"
     CDS             7917..8600
                     /locus_tag="U4A29_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO0680516.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9245..10435
                     /locus_tag="U4A29_001386"
     CDS             9245..10435
                     /locus_tag="U4A29_001386"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEO0680517.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10537..11490
                     /locus_tag="U4A29_001387"
     CDS             10537..11490
                     /locus_tag="U4A29_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEO0680518.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11559..12908
                     /locus_tag="U4A29_001388"
     CDS             11559..12908
                     /locus_tag="U4A29_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0680519.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13091..13414)
                     /locus_tag="U4A29_001389"
     CDS             complement(13091..13414)
                     /locus_tag="U4A29_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680520.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13543..14706
                     /locus_tag="U4A29_001390"
     CDS             13543..14706
                     /locus_tag="U4A29_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEO0680521.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14776..16125
                     /locus_tag="U4A29_001391"
     CDS             14776..16125
                     /locus_tag="U4A29_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO0680522.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16170..16601)
                     /locus_tag="U4A29_001392"
     CDS             complement(16170..16601)
                     /locus_tag="U4A29_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0680523.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16989..19631
                     /gene="alaS"
                     /locus_tag="U4A29_001393"
     CDS             16989..19631
                     /gene="alaS"
                     /locus_tag="U4A29_001393"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO0680524.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19831..20427
                     /locus_tag="U4A29_001394"
     CDS             19831..20427
                     /locus_tag="U4A29_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO0680525.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20549..21697
                     /locus_tag="U4A29_001395"
     CDS             20549..21697
                     /locus_tag="U4A29_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO0680526.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21735..22148)
                     /locus_tag="U4A29_001396"
     CDS             complement(21735..22148)
                     /locus_tag="U4A29_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEO0680527.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22227..22658
                     /locus_tag="U4A29_001397"
     CDS             22227..22658
                     /locus_tag="U4A29_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO0680528.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
BASE COUNT         7318 a         3963 c         4752 g         6629 t
ORIGIN      
        1 ttgttttcga taaaaaaaga tggtgcagac aaattatgat aattaagcat actatcagat
       61 aactcaaaat gaagccttct ttcaaaaaat tattcaaaac catatattgt attttttatt
      121 tgataataac aatgggatta ttaagaagtg gcactaaata aaagactagt gtgtatttag
      181 ccgatttaat tataaacaag taaatggata aagtatatgt aatgataaaa gaaaaatgga
      241 ttttctaatt tttaagctaa atgttagatc acacatgtat atgctggata gtcttataaa
      301 aaatcatgta atacagcttg gtacatgatt tttatttgta gctgtttttt acatagacac
      361 aaaaaagaaa ccacgcttgt agacgtggtt tcttttttac tatctgtttc cagaagctga
      421 ggtgctgctg gatggtgtat ggtcatttgc agcatccgct ccgctagttg ctggctcact
      481 ggaagaagca ggaggagcag atgaagacgg tgtcgagctt tcttcggatg aagaagaagg
      541 aaccgttgtt gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc
      601 agcagttgat tcgctttgac tagtagagct agagctttga gtcgttgtct ctgttgttga
      661 gcttccagga gtctggacat agctggaaga tggaatagtt gttgatggtg taattgcatt
      721 cgatctagct gtatattgat ctttgtagta cagttcaccg cccacacgaa ttaatccgct
      781 tggcatctcc cagtctgtat ttgtcacgtt agctgaaaca tactgcataa gctcgcgata
      841 aacatcagag gctacgtgtg acgattctga agtgacaggt gtcatttttt tattgtaacc
      901 tgtccacacg gaaatcgaat aatttggcgt atagcccgca aataggatat ctggatacac
      961 cccagaagaa ataccaagtt ttgcatactc gtcatctgtg taattggatg ttccggtctt
     1021 accggcttga tataacccag cgatttgcgc attcgtacca gtttcttcag tgatcgtatc
     1081 ttttaaaatg tctgtgatca tataagctgt ttctggagac atggctgttt ttccatctgg
     1141 ctcataagtt tcttctgttc cgtcttggaa gacgatttta ttaacatatt gaggtttgta
     1201 ataagtccct ccattggcga aagccgcata ggctgctgcc atttttaagg aagaagctcc
     1261 gtattttgtc ccatcttgtt cttccgtatt actagagatc gcattcgatt gatggatcgt
     1321 actgtattcg atcccaagat tttttaagaa agaagccact ttatctgaac cgacttcatt
     1381 gaataatttg actgctggaa cattacggga taagtaaagt gcttggcgta aagtaatggt
     1441 gcccatatat tgattatccc agtttcctac aggagtcgaa gtcccttcat agttatacgg
     1501 tgcgtcagta atagtttttc cagtagaata tttcaaatat tcaaaagctg gtccgtaatc
     1561 tgtgacaggt ttcatcgtag aaccaaaatc acgggaagta ttcactgcta agttgttgcc
     1621 taaagttacg tcttctgcga tgtgacgtcc gccaatctgg gcttttactt tcccggtatt
     1681 ggtgtcgata agggtggagg caacttgcat ctcgtcgtct gggtaagaga catactgatc
     1741 cgtgttgacg atatcatata gtttcttttg ggcgtccaga tccaaattcg tataaatctc
     1801 taaaccgtct gtgtagacat tcttatctgt tttttcttgt acttcattga tgacttcttt
     1861 gacataatta tcgacgatct tagtattgtc atcggattga gttagttctt gcagaccatc
     1921 cgttaccggt acattaacag cttgatcata ttctgtttga gagatttttt cgttttgaag
     1981 catcgtatac aaaacagtat ctcgtctttt cttcgcctga tctggataaa cataaggatc
     2041 ataggcggaa ggggcttgag gcatcccagc aagcaatgct gtttgcggta aagaaagttc
     2101 tgataatttc ttaccaaagt acatctcaga agctgtctcc atcccgtaca agccgtttga
     2161 catatacact ttatttacgt agtaggtcaa gatctcttgt ttcgattttt tttgttccaa
     2221 tcttacagcc atccacgctt cttgggcctt gcgtttcaga gtctgatcct cggcactggt
     2281 ggagaagaag gaaagtttga tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc
     2341 agaggtaaag ttagagaggg cagaaccgat gatccgaata gggtcaaccc cgatgtgttt
     2401 ataaaaacgg cgatcttcta ccgacacgat cgcatcttct aatgttttcg gtaattcatt
     2461 cgcagaaatt ttttcacgct tttctgcgcc taaatcttcg aatagttcgc catcttgtgt
     2521 atatagtttg gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt
     2581 tgcgtaatac cagaaaagtc ctacacctgc taaaaagagg atacagaaaa atgagagtat
     2641 tcctaaaagg atcttgataa ataatccttt tttatgtgtt ccagaggact ttccagaacc
     2701 tttgccagaa ttcttcttca cactcttttt aggagtaggc tgtttctgtc gtctagagct
     2761 tctagtttgt tcatttgcca ttcttgcttt ctccttcaat agataatgtt gataaataga
     2821 agtcaaccgc gtccaaataa ggaatccgcg gagaaatacc agttttgatc tctattccta
     2881 gttcttcgat caaagataaa ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt
     2941 aagcaatcaa atgaacacta ttcaaataaa aacagcgctt caaagacgaa aaccataaca
     3001 agacaaagca gaccccattt tgttcgaggc atgctttcat atgttcgatt tgatgggcat
     3061 ggaagttttt aaagggaaaa gatgttttgt tcttggtttc ttttgcttca aagtccaagt
     3121 aaaagcctcg atagacgcca ttatagtctg tagttgaggc ttgggtaaag taggcttcct
     3181 tgatcacagc agcacttctc ttaggatagt ctactttcac gatttggata ggggtaggct
     3241 ttttatggat aaccgccaac ccgcgggaaa gataatattt attactttca ttgattgctt
     3301 cttcaaagcg catcccccga ttgccgaatt cagtgatctg atttcttttt tcttgaattt
     3361 tttcgacggg agaggcattc ttataataag gttggccatt tggatagcgc aataccatct
     3421 ttaatcacac tcctaggcca cattatagca aaaaagcgga tagaaagaaa aggaatctga
     3481 gatggataag tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt
     3541 taaagaaaat gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag aactccatca
     3601 gttagcagaa gaagggctgg aatgggtact tgtcagtggg aatttgggcg tcgaactatg
     3661 ggctgtgcaa atagtagcgg aattgaaaga agaatatccg gaactatctc ttggtatttt
     3721 gtatcctttt tctgattttg gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt
     3781 agcagaacaa ttagcagatt atgtcgaagc tgttagccat caaccctacc aatcacctag
     3841 tcagctaaag aatcacactc gttttatact tgagcataca gtgggctgtc tgctgatcta
     3901 tgatgaagaa ttccctggaa aaacgaagta tttttttgaa gatgcacaga attaccaagc
     3961 agatcatccc tacgaagttc ggctgatcag tatggacgat cttcaaaatt ttagtgaata
     4021 atcgatattg tgtttgattt atcaactttt ttcctataca atgaaaaagg tagaaatgtt
     4081 attacgtaaa tgaaaactag aggtgtaaat atggcgaatt tggtttatag tcctaaagac
     4141 attttacaac aagaattcaa aacaaaaatg cgcggctatg atcccgttga agttgatgaa
     4201 ttcttagaca atatcatcaa agattatgaa acttacagca aagaactcct tgctttacaa
     4261 gaagaaaacg atcgattgag tgcaaaagta gctcaattat ctaaaactca aggagcagct
     4321 caaacacgcg tgcaacaaac agaggcacca aagagcgcag cagtgaccaa ttttgatatc
     4381 ttaaaacgct tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga tcaacaagca
     4441 agtgcagtca aaccagcaca acctaatcca aataactaca caaatgcaga cacaagctta
     4501 gatgataatg aaaaaacacg tcaattttaa tttttgatta atgaatttgc ggttttcggg
     4561 taatcgcggt ctgacttgat caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc
     4621 tgtagtgttc gtgcttagcg aaaccataag ctaaggtact ctttggagta acggcaggaa
     4681 aacaagctaa ggcttcggct atgcttaagt atccttgaaa gtgccacagt gacgaagcgg
     4741 ctggggaaac ccatccggtg gaacgcggta aacccctcga gcgagcaacc caaacaatgg
     4801 taggggcgct ttttgtctgg aagaagaaca ggacaaaaag gcagtataac tgcagataga
     4861 tggttaccgc tttgtttttt ctccctggaa aagcaaagta cagaacatgg cttatagaaa
     4921 accgcaaaca tcagggaatt aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa
     4981 acatttgaaa gctatttttc aatacttcag atgaatcgtt cttattacaa aacaaacttt
     5041 tgtatcaccg gttattcgga atttgagtaa aagtgtgggg taggactgat gtcccacctc
     5101 ctttttttat aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca
     5161 actgcggcaa gcgggctaga agcactggtt ggtaaagaat taagagatct tggaatcgaa
     5221 tgccaagttg agaacggacg tgcacgtttt gaaggaacaa tggaaacgat cgctacagct
     5281 aatttatggt tacgtacagc agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat
     5341 acgtttgatg aattgtttga aaacgtaaaa gcattgcctt gggaagattt tcttccacta
     5401 gatgcagctt ttcctgtagc tggaaaatcg atcaagtcga aactatatag cacacctgat
     5461 tgccaagcga tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca
     5521 gcttctgtac cactgacgga aactggcgca cattttcagt tggaagtagc tttgctgaaa
     5581 gaccatgtga tggtaactct ggatacgaca ggaccaagtt tattcaaacg tggttatcgc
     5641 ttagaaaaag gtggagcacc attgaaagaa aacatggcag cagcacttat tatgttgaca
     5701 aattggcgaa aagaccgtcc attttatgat cctgtatgtg gttcaggtac gctttgtatt
     5761 gaagcagcct taataggcca taatatcgca ccaggattca atcgtgagtt tgcttgcgaa
     5821 tcttgggatt ggttcagtca agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa
     5881 gcagattatg atattgaatt agatattact ggttcagata tcaatggacg gatgattgaa
     5941 attgccaaag ccaatgcaga agaaatcgga ttaggctctt cgattacctt taagcaacaa
     6001 gcagtcaaag acttcaaaac ggataaagaa tacggggtca tcgtggctaa tccgccttac
     6061 ggagaacgtc taggagaaga agaaactgtt cggaaattat ataaagaaat gggcgatgta
     6121 ttccgtccat taaaaacatg gagtaaatat atcttgacta gcgacttagc gtttgaggaa
     6181 ttttacggac aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt gcgtacggat
     6241 ctattccagt attggggaac tcgtccgcct agaaaaaaag agaaagacga ataatcttac
     6301 taattgattc attcaattgg atgtttggag gaatagggaa tgaaagaaaa agagttttta
     6361 caagaagtaa aagaaatcaa tcttctacaa caagctttag gaatcctcga ttgggataca
     6421 caaacaggta tgcctgaaaa agctagtgca taccgaagtg aagtagacag ctatctttac
     6481 agcatttatt tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg ctatttttct
     6541 gaacatccgg aagaattgtc ggaagttgga actgccgttt ttgccaaagt aaaagaagag
     6601 tatgatttgg aacatcgcgt accggaagat cttatgagaa aatatacagc tgccgtttct
     6661 tcagcacacg gacaatggca aaaagctaga gaaacacaac aattttctga ttttcaacac
     6721 gcattgaatc aaaatattga attaacaaaa cagttgatac cttactggag aaaagatgaa
     6781 aagacgaatt atgatgtttt gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt
     6841 gataaggtat ttgatcagct taaagaagga attctttcga tccgccgtac gctccaagaa
     6901 aaagggactg aacctgaaac agacttttta aatcggagaa tgacgaaaaa acaacagaag
     6961 agatttgtag tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat
     7021 gacactgtcc acccatttat gactggaatt aatccgaatg atgcacgaat cacgacacga
     7081 tggaacgaaa cagatttcaa aatggctgtt tttggagtga tccacgaggc tggtcatggt
     7141 atgtatgaac aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca
     7201 atgggtatcc atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc
     7261 ttctggaaaa agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt
     7321 ccttttgaca aattttatcg ttcattgaac catacaaaat caagtttggt tcgtatcgag
     7381 gcagacagtt tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt
     7441 ttcaatgaag gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag
     7501 tatctgggaa tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca
     7561 ggtgcaagct ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta
     7621 cttcattctt tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct
     7681 ccaatcaaaa actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat
     7741 caattaatca tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg
     7801 aaagacctgt attttaatgt atatcaggta aaataaaata caatttacaa agatagctga
     7861 aattcatcta atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg
     7921 ataaattaca agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac
     7981 atgggagcga tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac
     8041 cgcaggcaga ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata
     8101 aagtcgttaa aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc
     8161 caatagtaga ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta
     8221 aaaatctctc tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc
     8281 gtatcgaagc attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg
     8341 ggcacccgat ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc
     8401 gaaaaggcac gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa
     8461 tgaatactga ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct
     8521 tagaagaatt ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga
     8581 tcaaaaataa caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg
     8641 gatcattttc cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc
     8701 gttcagctcc gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat
     8761 tgtaacttgt gattcggaat tggcaggaga acaccgttta ttcggaagaa taggactgtg
     8821 acaagacttt tgtcacagtc cttttttcga attcaacaag taaaactttt aagaaatatt
     8881 cagagagtaa aattaaaaat tgctgataaa cgaagatgga aattttatgt atttttattg
     8941 gtctatgcaa taaataaatt aaatattcag aatacaattg attttaacga aagggcttct
     9001 atcaatggtt gtgttgtatt tatctgccca ggtagagtaa aatagatcat tatctaaaag
     9061 gtaattgttc atactatata agtcatttga aagtaaaaag atactgtaaa taaaaaatgt
     9121 ggttaaataa ttctcataat cagtgtaagc gttatcaaaa atatagcata cttgctttgt
     9181 agatgaatga cacgatatat gtttggtatg ttctgcaaga cagatgaata tggaggtgga
     9241 gacaatgtct tccaaagaac taacaatgta tttagagaaa gaattggatc aactgaaaga
     9301 aaaggggtta tacaatacga tcgacatact agaaagtgaa aatggtgctt gtatcatcgt
     9361 agacggaaaa aagatgatca atctagcatc caacaattat cttggatttg ccaatcgcga
     9421 agaattgaaa aaagcatgta ttgaagcaac cgaaacttat ggtgtaggag caggagcagt
     9481 ccgaacgatc aatggttcgc tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt
     9541 caaagggaca gaagcagcaa tcgcctttca atctggattc aactgcaata tgggcgctat
     9601 ctctgcagta atgacaaaag aagatgctat cttatcggat gaattaaacc atgcatctat
     9661 tattgacggt tgccgcttat ccggagcaaa aatcatccga attaaacatc aagatatgaa
     9721 agaccttgag aaaaaagcaa aggaagcgat agaaagtaag aaatataaaa aaatcatgta
     9781 tatcacagat ggtgttttct ccatggatgg agacattgca cgattgccgg aaattattcc
     9841 gattgttgaa aaatatggac tgatcaccta cgtcgatgat gcgcacggct caggcgtaac
     9901 tggaaaaggt gcaggaaccg tcaagcattt tggattaagc gacaaaatcg atatgcaaat
     9961 gggcacgtta tcgaaagcta taggtgttgt tgggggttat gtagctggat cgaaaacact
    10021 aatcgactgg ttgaaagcta gaagtcggcc attcctattt tctacttcgc taactccagg
    10081 tgcagctgca gctgcattgg cttcgattac tttgatgcaa gaacatccgg aacttgtcga
    10141 aaaagtttgg gaaaatgcta attattttaa agaagaattg aaaaaagtag gctataacat
    10201 cggtatgtct gaaacaccta ttactccagt cattttggga gacgaaaaag tgactcagac
    10261 tttctctaag aagctgatcg aacatgggat ttatgctaaa ccaatcgttt atccaactgt
    10321 gcctcttggg acaggcagga tcagaaatat gccgactgcg gaacatacga gggaaatgct
    10381 tgatgaagca gtagcagttt atcaaaaaat aggaaaagaa atggaaatta tttaactaaa
    10441 tttagttttt tcgtctattt aaaattagag aacatgttca cgcatatatt gttaatcatc
    10501 tgaagatagt agtaaaaaag atcagggagg atcatcatga aaaaagtatt ggtgacagga
    10561 tgtttaggac aaattggttc agagttgatc acacggctac gtcaagaact aggcagtgaa
    10621 aatgtcattg cgacagatat acgcatgcct gaagtcattg agaaaaacga tatctttgag
    10681 atcctagatg taatggatta cgaaaaaatg cgcagcatag cagaacaata tcaagtggac
    10741 acattgattc atttggctgc tcttttgtca gctgttgcag aagcaaaacc acaattagcc
    10801 tggaatttga acgtgactgg gctggtcaat gcattggaag tcgctagaga attccagctg
    10861 aaatttttta cgccaagttc aatcggcgcg tttggtccaa atactccaaa agatcatacc
    10921 ccacaagata ctcttcagcg tcctacaacc atgtatgggg taacgaaggt agctggagaa
    10981 ctactatgtg attattatta tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct
    11041 ggattgattt cttatgcaac cttgccagga ggcggtacga cagactacgc agtcgatatt
    11101 tattattcag caatcaaaga aggaaagtac actagtttca ttgatcaagg tacatcaatg
    11161 gatatgatgt atatgccaga tgcgatcgat gccattataa aactcatgaa tgcagatcct
    11221 gatcgattgg ttcatcgaaa tgccttcaat atatcggcga tgtcttttga cccggaagag
    11281 atcaaagcat ctattcaaaa aatcatacca gattttgaaa tggattatga tgtggatcct
    11341 gtacgtcagt caatagcaga atcttggcca aattcactag atgtttcttg tgcgcaagag
    11401 gaatggggct tttcgccgaa atacgactta gatgtgatga caaaggatat gttggaaaaa
    11461 ctgaaagaaa aattggtaga gacggtataa aacaagaacc acatccaaaa agttttttat
    11521 acttttggga ttggctatgg tatgaaaggg gacaagggat ggaagaatat caagccacgc
    11581 cagtaaaaga agtaaaaata gaaaatgaat taaaacgtac gatgggattt tttactgctt
    11641 tatcgactgt tatgggcaca gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag
    11701 cagaagttac aggatccgcc agtttgcata tgttttcttg gtttctcggt ggtatgattt
    11761 ctgtctgtgc aggactaaca ggagctgaac tggcagctgc tattccagaa acaggaggga
    11821 tgattaaata tatcgaacgg atttacggaa atacagcagc ttttttattg ggctgggcgc
    11881 aagtcgtgat ttattttcca gcaaatgtag cagccttgtc gatcattttc ggtacacagt
    11941 tcgtcaatct atttggtctt tcccagtcaa tgattgttcc cgtagctgtt acagctgctg
    12001 tatcgatcct gttgatcaat tttttaggtt ctaaagctgg aggggctttt cagtcgatta
    12061 cgcttgtctg caagcttatt cctttgtttg tgatcgtcat tttcggtttg ttccgacaag
    12121 aaggagtaga cttccaactg tttccgatcc aagcaggaga aaatctttca tttttctcag
    12181 ccttgggagc gggcttgcta gcaacgatgt tcgcatacga cggttggata cacgtaggaa
    12241 atatttcggg agaattgaaa aagccggcga aagatttgcc aaaagcaatt tctttaggaa
    12301 tcatcggaat catgatcgtt tatttattag taaatgcggt gttcttaaga accgcatcta
    12361 tcgacggagt cgcaggaaac agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg
    12421 gcttcggtgg aagactggtc acagttggta tcttgatttc tgtttatgga accatcaatg
    12481 gctataccct tactggaatg cgtctccctt atgttattgc aaaagaaaac aacctgcctt
    12541 ttagcaagct ctttgctaaa cttcatgata aaactaaagt tcctgtagcc gcaggtatct
    12601 tagagctagt catcgctatt ggcatgatga tgatcggtgg ttttgataca ctaacggata
    12661 tgctgatctt tgtcatttgg attttttata cgatggtctt tgttggtgtc attcttcttc
    12721 gtaaaaaaga acctgatttg tttcgaccat acaaagttcc aatgtatccg tttattccac
    12781 tagtagcaat cattggaggg acttttatcg tatcaagcac gcttatcact cagacctttt
    12841 tagcttcgat ggggatagta ttgacacttg ctggtatccc gatttatctc tatttaaaac
    12901 gtagatagct aaaacattcg aattttatta gaactgcact tctctgccaa acaaaatatt
    12961 ttatttaaat gaggagtacg tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt
    13021 aaatgtcgtt ttgtctacga tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga
    13081 tttctgtctt ttactttgat ttgcgtaact gaactattcg gcgtacgccg cgataaatat
    13141 ttaataaaac caagcaacca gaaatgaaaa tgggaagcca aatcagatag acagggtagc
    13201 caagcacttt agacaaagca tgtggagtca ttttatccat ttcaagaagt gcttgctgcc
    13261 aagtctgaag attgaagact agcatgaaac ccgaaataat caggagaata aatcctaaat
    13321 aatgaattgt agttcttttt cttacataag cgatcgtagc ataaaggaac agtaaaaaaa
    13381 taggtacgcc aaaaacaaag tacatattca gcatctcgac accccctttg tgtttatctt
    13441 actttacttt gaaagaaatg agaaataata gcctcgttac ttgcttgaaa cagcaaagga
    13501 ctgctaagat aagagagata caaagaatca aggaggagtc atatggcaag ttatatcgaa
    13561 ccgattcgtt ttgcgctgat tgtttttcca tttttagcct tagcgatctc atctgttttt
    13621 tttatttatg aatatcgaaa gtatggaaca tttctagtaa caagggcagt tattttgtat
    13681 tctttcgttt tttatttatt gtgtgcgtat tttttggtga tcttaccact tccggcaaag
    13741 gagacggttg cccaaatgac agggccaaag atggagctgc atttatttgc ttcatggcaa
    13801 catttcagag acaacacggt attggtcttg tctgaaccta gtacctacct gccggcaatg
    13861 aagcagagtg tatttttgga accagctttt aatgttttat tactcatacc atttggcatt
    13921 tatttaagat attatttccg gctttctttt atgaaaacga tgattgctag tttctgcctt
    13981 tctctatttt ttgaattgac gcagctaagt ggactttatt ttatctaccc aagaccatac
    14041 cgcttatttg atgtgaatga tttagtgaca aatacattgg gtggaatcat tggttttgtc
    14101 gttacgccgt tatttacatt tatgcttcct acaagaagac gaatggatga actttcttat
    14161 gaaaaaggaa agactgtctc attgactcga cgcttggtcg cttatttgat cgactggaca
    14221 tttttatctc ttattaattt ggtagcggcg attttcttta aaattgcgtt cgattcaggg
    14281 gcagttactt ctcatatctg gtggctttta ttagaagtag gggtatactt tatgattgta
    14341 ccgtttataa ccaatggatg gacacttggc aaaaaaattg tgcgtatccg tttggtggaa
    14401 gaagaccgcg aacgaatttc tttaaaagca ttgatgatcc gctacgggta tctgtacttt
    14461 cttttttatg gtttaagctg gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg
    14521 aatacggtgt tgcagatgag tgcagtattc atttctctcg gtgtcttgtt actacaaggt
    14581 atttttattt taaatatctt gctatcattt ttgagaaaaa aacggattct gttttatgaa
    14641 aaagctagtc acacatatac tgttagcacg attggccaaa caaaggatga tgattctcga
    14701 aattagctga gcaaaaacgc tttgtctgtg ttataatgcc aaagtcgtga taatttaaag
    14761 agaagaggtg cacgaatgaa taattttgat atattccgct tcaaaccgtt tatcaatgaa
    14821 gcgttaaccg caaagggctt caaacagccc acagaggtcc aggaacgttt gattccttta
    14881 attgcaaaag gaaaaaatat cattgggcaa tctcaaactg gtacaggaaa aacacatacg
    14941 tttttgttgc ctttgtttga aaaaatcaat gcagaaaaag aggaagtaca agttgttatt
    15001 acagctccaa gtagggaact agctgcacag atctatgaag cagctacaca aatcgctaaa
    15061 ttttctacac cggagatccg tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa
    15121 ttaaatcgtc tgcatcacca acagccacag gttgtagtag gcacgcctgg tagaatatta
    15181 gacatgatga atgaacaagc gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa
    15241 gcggatatga cattggatat gggtttttta aatgaagtag accaaattgc aagtcgatta
    15301 ccagaaaagc tgcagttttt agttttttct gccactattc cagaaaaact tcgtccattc
    15361 ttacgaaaat acatggaaaa tccaatcatt gaagaaatca aaccaaaagc agttatttct
    15421 gaagacattg aaaattggct gatttcaaca aaaggtaaag atgagaatca aatgatttat
    15481 caactgctga caattggtca tccttattta gcaatcgtat ttgccaatac aaaacagcat
    15541 gtggatgaaa ttgccgatta cttgaaaaac caaggcttga aagtagcaaa gattcatgga
    15601 gacatcaccc caagagagag aaaacgagtg atgcgacaag tacagaatct ggagtatcaa
    15661 tatgttgtag cgactgattt agctgctaga gggatcgata tcgaaggtgt ctctcatgtt
    15721 attaatgcag aaattccaga agatctagac ttctttatcc atcgtgtcgg acgtactgga
    15781 cgtaatggct taaaaggaac ggccattacg ttatattcgc caaatgatga gcaagccatt
    15841 gatgcgatag aaaaattggg cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc
    15901 gtcaaaacat acgatcgtaa tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat
    15961 cccacgctga ttggtcttgt gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa
    16021 aaaatcgatt gggcaattgc agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag
    16081 caaagccgta ctgctcgtaa aaataagaaa aattcacata aataaaaaac aagaggttgt
    16141 gacaaaaggc gtgtcacaat ctcttgtttt taagctagac tttttttcat tccgatacaa
    16201 agtacttcat cttcaataaa aggttctgtg caagtatgat acccttgttt ttcataaaaa
    16261 tctacagccg tcatttcagc tactacataa ctagtctgac aaccttctga aaccgcccgt
    16321 ttttcagcag cttcaagcaa ctgaccgcct aacccttttc ctcggtattc ttttgctacg
    16381 caaaatcgtt ccggcttgaa gcaagtaggt gttttctttt cataacgcaa agtagctact
    16441 ggtctttttt gatcaagaaa tagataatag gtttgttgcg ggatatcttt tccgtcgaat
    16501 tctagttctg caggaatccc ttgttctaat acaaaaactt gatatctcaa aaagcagcta
    16561 gctgcgcgta tccattcatc cgctccaact atttttttca tcatattcct ccttgtcatg
    16621 aatagcagag atgtattgat tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc
    16681 aaaaggaaat gaaaataaaa attgagatat tgacagtgat caatagaatg cgtatactaa
    16741 ttaagtacga aaaggatatg atttgttttt taagactcac agaaaattct ttcttggctg
    16801 aaaaaagaat agtcactaat caaattgctc cctttttcta ttttggtaga aataccgacg
    16861 cagctcagcg ttatcgagtt taagaggtaa tgattacaca tcattgcaat caaggtggta
    16921 ccgcgagttt tcgtccttgg ctgtgatggt gtttttttgt atttaattta tagaaagcag
    16981 gtaatgacat gaaacaatta agtagtgcac aagttcgaca aatgtttctt gatttcttcc
    17041 aatctaaagg acatacgatt gaacctagtg cgtctttgat ccccgtgaac gatccaacct
    17101 tattgtggat caactctggt gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc
    17161 cagaaaatcc aagaatcaca aatgcccaaa aatctattcg aacaaatgat atcgaaaatg
    17221 tcggtaaaac agcacggcac catacgatgt ttgagatgct gggtaatttt tcgattggtg
    17281 actatttcaa aaatgaagcc atccactggg catgggaatt tttgactgat ccaaaatgga
    17341 tggcattcga tccagaaaaa ctttatgtaa cagtttatcc aaaagataca gaagcaaaac
    17401 gcatctggca tgaagaagtt ggcctgccac tagatcacat tattgatgta gaagacaact
    17461 tctgggatat cggtgcaggc ccatgtggtc cggatacgga aattttctat gaccgtggag
    17521 aagcttataa cgatttagca gaagacgatc ctgaaaacta tccaggagga gaaaatgaac
    17581 gttatttaga aatctggaac ttagtatttt cagaattcaa ccataagcct gataacacat
    17641 ttgaaccact tcctcataaa aacattgata cggggatggg attggaacgc atggtctcaa
    17701 tcgttcaaga tgcgtcaacg aactttgaga cagacttgtt tatgcctatt atccacgccg
    17761 tagaaaagct aagcaaacaa gtaaaatatg gtgaaaaccc agtaacagat atttcttaca
    17821 aagtaatcgc agatcatatt cgcgcccttt catttgctat tggcgacggt gcattgccat
    17881 cgaatgaagg gagagggtat gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga
    17941 aaaaattagg tattgaacaa gcattcttat atcgtttagt ccctgttgtt ggggaaatca
    18001 tggtaagtta ttatcctgaa gtacttcaac aaaaagaatt tattgaaaaa gttatccgta
    18061 cagaagaaga acgtttcaat gaaacaatca atgaaggttt ggaaatctta aatcaagtga
    18121 tccaagaagt aaaaaacgaa aacgggacga ctctagcagg aaaagacatc ttcaaactat
    18181 acgacacata tggcttcccg gtagaattaa cggaagaggt ggcagaagat gaaggactga
    18241 aagttgatca tgctggtttt gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg
    18301 cccgcagtac tgaaaaatca atgggggtac aatctgcttt attgacagat atcaaagtag
    18361 aaagtaaatt cattggatac acagaacaag aatcagatag tcaattattg gtcatcgtta
    18421 aaggtgagtc tcttttgaat gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa
    18481 cacctttcta tgcagaaatg ggtgggcaag tagcggatca aggatggatc atgaatgaag
    18541 taggcgaaat cgtagcagtt gttacagatg ttcaaaaagc gcctaatggt caatttatgc
    18601 acaccgtaga agtaaaagca ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc
    18661 aatcgatgcg caatcggatc atcaaaaacc atacagcaac ccatctactg cacaaggcat
    18721 tgaaagaagt acttggcgag catgcaaatc aagctggttc tttagtcgct cctggacatt
    18781 tacgattcga ctttacccac tttggtcaaa tcacttcaga tgaattagct gaaatggaaa
    18841 gaatcgtcaa cgaaaaaatc tgggaagcta tcccagttga aacaattgaa acagatatcg
    18901 atacagccaa aaacatgggt gcaatggctt tgtttggtga aaaatacgga catgacgttc
    18961 gagtagtaaa tattggcggc tggtcaatcg aactgtgcgg aggcacacat gtagcaaata
    19021 cagaaaacat cggaatcttc aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac
    19081 gaatcgaggc tgtaacaagt aaagaagcgt acgaactact tgaagaagaa gaaaaacaac
    19141 taaaacaagt agcaacaatt gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg
    19201 aacaattgca acagcaagta agagagctac aaaaggaaaa tgaacaatta gctagcaagt
    19261 tagcgaatca acaagctggt gatgtattca aagatccaga aacgatcaat ggaaaaacaa
    19321 tcattgcagc acaagtaaaa gtgaaagata tgaatcagct gcgtcaatta gctgaccaat
    19381 ggaaacaaaa aaatatttca gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca
    19441 gtctgctagc ggcaatgacg aaagaatcaa atgaagctgg actaaaagct ggcgacttga
    19501 ttaaagcaat cgcaccgaaa gtaggcggtg gcggtggtgg acgtccagac atggcacaag
    19561 ctggcgggaa aaatcctgct ggtattccag atgcacttaa agctgcgaaa gaatgggttg
    19621 cgcaacaata gggcgtttac gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca
    19681 tgattatggg ggcgaagaca aacaagtctt gccctcatac ttttttcaat tggatggcgc
    19741 atctaatcaa atggtcggat tcggatggtc tttagttgac gaaagtgaca gaaattttta
    19801 cgattagtat aaataaaaag gggtgaaaaa atgaatagta gaaataagac ctttcgacta
    19861 gtcttacgtg cgatcttgtt agcaatcatc atcgtacaag cgatggttcc ttggctgggg
    19921 tttattccat taggattcat tagtttaaca attatccata tcacagtaat tattgctgca
    19981 gtagtattag gcccaaaaga tggcatggtg attggcttgt tttggggaat cgctacaatt
    20041 gttcgtgcct atgccatgcc aacaacgcca tttgatacat tagtgttcac gaatccgatt
    20101 atctcggtag ttcccagagt acttgttggc ttagtagcag gcttggtttt ccactggatt
    20161 tatcaaaaaa ataaatcaat aacgatcagc tcagcctttg caggggtact gggttcattg
    20221 gtcaatacag tcttagtgtt aggattcatg ggattatttt atacaggtgc aacggcagat
    20281 gcctatggtg tagactctgc tcttttattt aaaaccttag caggggttgc agcaatcaat
    20341 gggataccag aagcaatagg tgcgggaatc ataacaccat tactagcaaa agcgttattt
    20401 gcagcaacac cgttgaaacc agaataatga ccatctttta atagtaatag gacgcacgat
    20461 agatagaaaa aggaagagag gagaggaaaa caatcgtcct ctccatcttt ttacctgagc
    20521 aacaagcaaa ctagaagggg taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca
    20581 aataccgcaa cagcggacaa aaaaaggagt actagcgggt atcttaggat tggttacgaa
    20641 tctgatcttg tttgtctcta agctaatgat cgggcttttt tcaggaagtg tctccattat
    20701 ggctgatgca gtgaacagtc tatcagatac tgcttcatcc atattgactt tgatcggatt
    20761 taaaattgct gcaaagccag cagatcagga acatccttat ggacacgaac gcttcgaata
    20821 tatcagtggg ttatttgttt cgatcatcat tacttatgta ggatttcaat ttttagagtc
    20881 ttctgctgaa aaaatcatcc atccagaaaa tgtctcttta acacccatcg tcttttttgt
    20941 cctaatcatt tccatctttt tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca
    21001 atccattgat tctgaaacat tgagagctac agcaacagat agttataatg atgtctttac
    21061 tacactggct gtattgcttt cagcagcaat cgaatgggta actggatggc gtattgatgg
    21121 atatataggt tttgctttag cattatatat cctttatagt ggattgacga tgcttcgaaa
    21181 ttttgtatac gaactattag gaagtcgccc aacgccagca gagattgaag aaatggaaga
    21241 gcatttaaat gcttgcgatg atatacttgg ctatcatgat ttattggtgc ataactatgg
    21301 acccaataaa cgctttgcat cagtccacat cgaagtagat gaatcaatga atttgaatgt
    21361 ggctcataag attattgatc tgatagaaaa agactttaaa aagtctttgg atgtggaact
    21421 tgtctgtcac ttggatccaa tgcctgttga taacgaaaaa tactataata tcttgcggca
    21481 gttaaagcaa attcttcttg aaacaggtac agggcttaat attcatgatt ttagagtaat
    21541 tcatcaagga aaaacgctgc aatttgatgt agtggtccct gagaaattcc agtttgttga
    21601 tacagaacta gaaagaatct tacgtagtca aatagaagaa aaaatcggag attaccaatt
    21661 ggaaattacg tttgatcaca attatctttt atattaaatg agaaactata aaaagactcc
    21721 ttaaagtttt taatttaaag agtctttttt tcttttaatt gtttatggag agcctgacgt
    21781 gcaagattat ctgctccttt atttttgttt tcgggcaacc attgcaggat caataaagaa
    21841 aattcggata gaagttcttg gattctttga aggtagggaa ggaagagctc gttcttcgtt
    21901 gcgtttttgt cgaccgtaga aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca
    21961 ttctgtaagt cgtttttctt tagaaattct aaagttttta aaaagacttc gaactctgct
    22021 tgatggtttg tccctataga aagcgggata gatagctgtt cttgtttacc ttgatagaca
    22081 atcaagattc cacctccact aggaccaggg ttgccttttg tagaggcatc aatgtatact
    22141 tttaacataa aaagagattc tcctttttgt tcaaactaat gttatggtaa gattatatca
    22201 ttactgaatc ggtgagaggt tagagaatga aaaaaaatat tcgatggcag cctgaactgg
    22261 cacatacgat catctattgg tcattgacct tgatcattct gttttttagt ctgattcttt
    22321 ctttagaaaa cacaggtcct tattggaaaa gcaattttgt tttaggtatt tttcttgttt
    22381 ttctatttct aggtttgcgg cgttctttta ttttaaaatc aaactctgtg attatacgtt
    22441 atgctgcttt ttggaaagac cgagaaatat ctgtcggaca gattcgatct ttgacgccaa
    22501 ctaatactgg ggtgcaaatg atgttgcaag atgagaaagc cccatctgtt ttcatgatgc
    22561 gaaaaaaaga tcaagtagtc ctttttgatc attttgaaaa aaacggaccc aaatcagaat
    22621 caattcctta tccttatacg aaaaaaagaa aaagctaatt tt
//
LOCUS       DASOUD010000040        21309 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.040, whole genome shotgun sequence.
ACCESSION   DASOUD010000040 DASOUD010000000
VERSION     DASOUD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.040"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..808
                     /locus_tag="U4A29_001398"
     CDS             68..808
                     /locus_tag="U4A29_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO0680529.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="U4A29_001399"
     CDS             complement(1232..1402)
                     /locus_tag="U4A29_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO0680530.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="U4A29_001400"
     CDS             complement(1596..1775)
                     /locus_tag="U4A29_001400"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680531.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="U4A29_001401"
     CDS             2162..3274
                     /locus_tag="U4A29_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEO0680532.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="U4A29_001402"
     CDS             complement(3352..3645)
                     /locus_tag="U4A29_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEO0680533.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="U4A29_001403"
     CDS             3813..4127
                     /locus_tag="U4A29_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEO0680534.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="U4A29_001404"
     CDS             4148..6127
                     /locus_tag="U4A29_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO0680535.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="U4A29_001405"
     CDS             6140..6466
                     /locus_tag="U4A29_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEO0680536.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="U4A29_001406"
     CDS             6482..7000
                     /locus_tag="U4A29_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680537.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="U4A29_001407"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="U4A29_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEO0680538.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="U4A29_001408"
     CDS             complement(8436..9941)
                     /locus_tag="U4A29_001408"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO0680539.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="U4A29_001409"
     CDS             complement(10033..10623)
                     /locus_tag="U4A29_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO0680540.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="U4A29_001410"
     CDS             10802..12448
                     /locus_tag="U4A29_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO0680541.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="U4A29_001411"
     CDS             12497..13216
                     /locus_tag="U4A29_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO0680542.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="U4A29_001412"
     CDS             13695..14642
                     /locus_tag="U4A29_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0680543.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="U4A29_001413"
     CDS             14654..15574
                     /locus_tag="U4A29_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO0680544.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="U4A29_001414"
     CDS             15615..17072
                     /locus_tag="U4A29_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO0680545.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="U4A29_001415"
     CDS             17417..18031
                     /locus_tag="U4A29_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEO0680546.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="U4A29_001416"
     CDS             18024..19748
                     /locus_tag="U4A29_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO0680547.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="U4A29_001417"
     CDS             19745..21196
                     /locus_tag="U4A29_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0680548.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6869 a         3608 c         4317 g         6515 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgg
//
LOCUS       DASOUD010000041        21097 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.041, whole genome shotgun sequence.
ACCESSION   DASOUD010000041 DASOUD010000000
VERSION     DASOUD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.041"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="U4A29_001418"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="U4A29_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEO0680549.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="U4A29_001419"
     CDS             complement(2017..2919)
                     /locus_tag="U4A29_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO0680550.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="U4A29_001420"
     CDS             complement(2916..3608)
                     /locus_tag="U4A29_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEO0680551.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="U4A29_001421"
     CDS             complement(3651..4292)
                     /locus_tag="U4A29_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO0680552.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="U4A29_001422"
     CDS             complement(4330..4644)
                     /locus_tag="U4A29_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680553.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5984)
                     /locus_tag="U4A29_001423"
                     /pseudo
     CDS             complement(4632..5984)
                     /locus_tag="U4A29_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5981..6913)
                     /locus_tag="U4A29_001424"
     CDS             complement(5981..6913)
                     /locus_tag="U4A29_001424"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEO0680554.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="U4A29_001425"
     CDS             complement(6910..7686)
                     /locus_tag="U4A29_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEO0680555.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="U4A29_001426"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="U4A29_001426"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO0680556.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /gene="carA"
                     /locus_tag="U4A29_001427"
     CDS             complement(10930..12006)
                     /gene="carA"
                     /locus_tag="U4A29_001427"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO0680557.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="U4A29_001428"
     CDS             complement(12011..13291)
                     /locus_tag="U4A29_001428"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO0680558.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="U4A29_001429"
     CDS             complement(13310..14236)
                     /locus_tag="U4A29_001429"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO0680559.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="U4A29_001430"
     CDS             complement(14249..15532)
                     /locus_tag="U4A29_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO0680560.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="U4A29_001431"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="U4A29_001431"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO0680561.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="U4A29_001432"
     CDS             complement(16320..17231)
                     /locus_tag="U4A29_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO0680562.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /locus_tag="U4A29_001433"
     CDS             complement(17228..17692)
                     /locus_tag="U4A29_001433"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO0680563.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="U4A29_001434"
     CDS             complement(17697..18395)
                     /locus_tag="U4A29_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO0680564.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="U4A29_001435"
     CDS             complement(18382..18768)
                     /locus_tag="U4A29_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680565.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="U4A29_001436"
     CDS             complement(18915..20585)
                     /locus_tag="U4A29_001436"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO0680566.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="U4A29_001437"
     CDS             20768..21001
                     /locus_tag="U4A29_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEO0680567.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
BASE COUNT         5900 a         4744 c         3743 g         6710 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaacgata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaa
//
LOCUS       DASOUD010000042        20947 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.042, whole genome shotgun sequence.
ACCESSION   DASOUD010000042 DASOUD010000000
VERSION     DASOUD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.042"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1258)
                     /locus_tag="U4A29_001438"
     CDS             complement(<1..1258)
                     /locus_tag="U4A29_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0680568.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFI"
     gene            complement(1224..2702)
                     /locus_tag="U4A29_001439"
     CDS             complement(1224..2702)
                     /locus_tag="U4A29_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0680569.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(3007..4842)
                     /gene="tdc"
                     /locus_tag="U4A29_001440"
     CDS             complement(3007..4842)
                     /gene="tdc"
                     /locus_tag="U4A29_001440"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEO0680570.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            5312..6739
                     /locus_tag="U4A29_001441"
     CDS             5312..6739
                     /locus_tag="U4A29_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0680571.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(6831..7196)
                     /locus_tag="U4A29_001442"
     CDS             complement(6831..7196)
                     /locus_tag="U4A29_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEO0680572.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(7193..7558)
                     /locus_tag="U4A29_001443"
     CDS             complement(7193..7558)
                     /locus_tag="U4A29_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEO0680573.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(7750..7998)
                     /locus_tag="U4A29_001444"
     CDS             complement(7750..7998)
                     /locus_tag="U4A29_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680574.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            7997..8125
                     /locus_tag="U4A29_001445"
     CDS             7997..8125
                     /locus_tag="U4A29_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEO0680575.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            8182..9876
                     /locus_tag="U4A29_001446"
     CDS             8182..9876
                     /locus_tag="U4A29_001446"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO0680576.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            9880..9999
                     /locus_tag="U4A29_001447"
                     /pseudo
     CDS             9880..9999
                     /locus_tag="U4A29_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            10527..11660
                     /locus_tag="U4A29_001448"
     CDS             10527..11660
                     /locus_tag="U4A29_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEO0680577.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            11691..12011
                     /locus_tag="U4A29_001449"
     CDS             11691..12011
                     /locus_tag="U4A29_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEO0680578.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(12040..12504)
                     /locus_tag="U4A29_001450"
     CDS             complement(12040..12504)
                     /locus_tag="U4A29_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0680579.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(12636..13889)
                     /locus_tag="U4A29_001451"
     CDS             complement(12636..13889)
                     /locus_tag="U4A29_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0680580.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(13918..14235)
                     /locus_tag="U4A29_001452"
     CDS             complement(13918..14235)
                     /locus_tag="U4A29_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO0680581.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(14261..14581)
                     /locus_tag="U4A29_001453"
     CDS             complement(14261..14581)
                     /locus_tag="U4A29_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0680582.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(15064..16479)
                     /locus_tag="U4A29_001454"
     CDS             complement(15064..16479)
                     /locus_tag="U4A29_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO0680583.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(16496..17812)
                     /locus_tag="U4A29_001455"
     CDS             complement(16496..17812)
                     /locus_tag="U4A29_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0680584.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(17962..18660)
                     /locus_tag="U4A29_001456"
     CDS             complement(17962..18660)
                     /locus_tag="U4A29_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO0680585.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(18949..19905)
                     /locus_tag="U4A29_001457"
     CDS             complement(18949..19905)
                     /locus_tag="U4A29_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO0680586.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(20018..20851)
                     /gene="msrA"
                     /locus_tag="U4A29_001458"
     CDS             complement(20018..20851)
                     /gene="msrA"
                     /locus_tag="U4A29_001458"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO0680587.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         6596 a         4299 c         3538 g         6514 t
ORIGIN      
        1 gaataaagcg tgtcgtgctt tcgaataaca agctgaaagc aattgaagta aaaaacacca
       61 acaaagcaag atctttatat ctttgtgtag tgttccgttt cttttcttta ataaattgaa
      121 catgtgcaca caagatcgaa gaccacacaa atagaaaaca actcgttgtc atgcctgcca
      181 aaagctgaaa aaacaatcga ctatcagcta caaaaaaatg tagacaaaga ccggcaaaaa
      241 ataacattcc tgtaattaaa ataccgtgta aaggtacgcc tttcttagaa agcgcacaaa
      301 accaatgggg caaacttttt tcttttgctg tttgatataa aatcctgcta gtactgtaga
      361 ctgcactatt tgctgaggac aaagaagcaa ccaaaatcat tagattgaca attgctgctc
      421 cgccttggca atggatcact tgcagcatag tcactaaagg actttctttt aaatctaaac
      481 aatgccatgg aacaacaaat aaaatcgaca aaatcgttcc tatatagaaa aagagaatac
      541 gccaaggcag acagttgatt gcttttttca aattcttttg cggatcactt gtctcgccaa
      601 cggttaagcc gatcatttca attccagcaa aactgaataa aaccatagga aatgagacta
      661 ccattccagc aaagccaaac ggtagtaatt catcgatctg tccaaaattt tctttgattc
      721 cgatagactg ggtatgaaaa tctgagacac tcaatcctat acccaataag ataaaaagga
      781 aaatccccaa gatcttgatg attgagaaaa aagattccaa ttcaccaaac cacttgactg
      841 caacaaggtt gactgccatt aagatcatta gtgcgctacc tccgcttatc aaagctggta
      901 catgtacaaa ccagaaatgg atatacaatc ctattgctgt taattccgca agtccagctc
      961 caagccagca aagccaataa gtccaattga caaaaaatgc ccactttttg cctaaataat
     1021 agtcaacgaa atcactgata gagttaaatg tactatttgc taaaaataaa tcacccatgg
     1081 cccgcatcaa accataaata gccaaaccgc atatcaaata aacgagaata acagctggcc
     1141 ctgctgatgc aattgccttt gctgaaccta aaaataatcc tgttccaatt gcgccaccta
     1201 atgcgattaa ctggatattt cgattagtta gagtccgttt cattgccggt ttttccatca
     1261 agttcacctt tttctttttc aagcagcatg gcgacttcct gttcccaaga aggtttcttt
     1321 attttttcaa tacagagtgg tactacaaac aaaatgacac tgatggctgt gactgcaatt
     1381 agattgatcc atgttccagc tttcatcgtc agtccgacag aaaatccgat tgccgcaaat
     1441 aaaacaataa cgacagtcca aactccaaga ttgctctttt tctctttttt tccaattcta
     1501 aatggtcgat tcaactttgc atgtgtataa cgcaatctca aaactgcgat ccccatgatg
     1561 acatagacga tacagtagat aacagtagtt gaatttacga gcatcaaaaa tgcttgattg
     1621 acgccaggaa tcagcaaata aaaaagtgca aagattgaga taatgacaga ctgagtcaaa
     1681 ataacggatt tggatacgtc aaattgattc gtcttccaaa aattgaattt tggtggatac
     1741 gcaccttttc tagcactttc tgtcactgtc tttgctggtc ctgaggccca agcagataat
     1801 tggactgcta caccaatgaa gacaagtaaa ctaaacagat tcacaataag atgtggcaaa
     1861 ccaagaattt ggcaataaat cgaaatagat tgcgcgatat tattcaattc catttgtcct
     1921 tttggtacga catttgctac aactaaagca ttcaaaatat tgacgaaaaa tataaaaatt
     1981 aacgcggcaa atactccgcg aatatacgtt ttaactggat tttccaatcg agtgatatac
     2041 acagaggaca tttcgatacc agtaaaaata aacatgatcg gcgcaaaata aacaaatgag
     2101 gatgcagtcg tagtatctgg tattaatttg tcccatgaaa aactgcctaa tgttccggtt
     2161 ggtacgatcc caactttgat ccacgcagca aaccctaata agagcatcat cgtcaaaggc
     2221 acgtacaatc ctaaccaaac accgactttt ccgccgattt ttgccatatc aaatttcaaa
     2281 ttcaaaaaag tgatgatcca ataaacgact aaaatgacaa ctaatgtaaa tttgctgttc
     2341 aaaccaagcg gcacattatc gataatatta ccaaacaacg gagcgagtgc tgaagcgacc
     2401 atcaccatcc caggaaacat ttggacccat aacagccatg agacaacgaa cccccatttt
     2461 tttcctagag cattcgatac ccaaagttct ggaccacctt tttggggaaa cattccagca
     2521 aattcaccag agatcaatga gattggaaaa gcgtacaata atactgcaac cagcatgtaa
     2581 aaaatcatcg tccagctcgt tgctgctaca tctggaatat ttcgtacact tgctacaagg
     2641 gcgcaggtca ttccgataaa ggtcatggtc gtgatttgct ttgatgaacc agtatctttc
     2701 atctttattc cttctttcaa tagcgacata tgtttttgtt ttcttttatt tttgttcaag
     2761 aacagaaatg acaaggtttc atcgattcag tacaaaaccc agtatcaccg aaacatccat
     2821 tgaacgatct tagtgaactg ggtttgatac atgaaacgaa cgatccaact tatagaacgt
     2881 tttataaaaa agaatatctt ggcgaggaat atatgaaaaa cagggggaaa tacaattgtt
     2941 ttgatgaagc aaataattgt ctgttctcca tatgtctctt gttattcttt cacacaaaaa
     3001 tcagttctat tggatgattt cgtttagttt cttttccatt gctttggcga ttttttttgt
     3061 ataaaaattg aaaattcgat catcattcaa ataaggagtc atgatagccg cacgtagcag
     3121 agtgattttc tgttcttttt tccattcttc tttcgttaga cccattcttt caacaaaacg
     3181 aatcggagaa tcaccgtaat cttcttgtgt aaatgtagta tgagaagtaa taaaccgttc
     3241 accataaaca tcgccatcca tataagaaga ccgatcgaac attttttcat ttaattcatt
     3301 gatagcgttc aaatctgtac agccttgttc tttgaatacc caattgacca tattaaaatc
     3361 aggatgattc aatggataga cttcgattgt ttttccttta acagtaaaag tcagatgatc
     3421 gagaaattct ctgaatcttt gagctgcctc gattgaagcc ccaatcaatt gaccatatcc
     3481 ggtcacattc aatggcaata cacgatgtgc tgtccagact gcggcggcgg ttgctccagc
     3541 ttttgatcct tccaaaatat aagctcctaa catatctgga gcctttactg atttttcaaa
     3601 cacatatggc gcgaaataag aaatgatatt tctcatattt ttatgtttga ttacgattcc
     3661 tccagcagca taagggacat agcccatctt atgaggatct atcgtgacag aatccgcttc
     3721 tgaaatagct ctgaaccctt catatacctc tttgtcaatc gttacgcagt ggtggaaaac
     3781 atgatgttct tcaaagaatt cagctaagga agcatacggc acaaattctc ctgcttcgtt
     3841 tagaaacagt gaacgcgcat agccgccata tgcggcatca acatgtaaat agaaatagat
     3901 cccttcatct tttaaccttt ctcttaattg gacgatttta tctacactgt ctacttgacc
     3961 ttcttctgtc gttcctacga cagcaaccac acctagaatt gggatttttt gtccagctaa
     4021 ttcacgaatt gttttttcca aagcattaat atccattcga taatcctctt gaactgggat
     4081 agcaaccatc tgatctaagc cgactccgca aatatctaac gccttcatcc aagaatagtg
     4141 ttttgtttga gggaccagcc atttccctaa tctttggata ttttttccgc tacgggaaga
     4201 agctgctttg acctcatcca tttcttcgtc cgtaaagctt tcggtcattt ccaaaatttc
     4261 ttcaacagat aaattcaaaa gttcccactc agacattttt ttcacttttt caggcagcac
     4321 ttccttgaca gctaaaggaa tcgatttgat gcaacgggcg taccataatc cttcaagatt
     4381 agcaatagaa ccatctgctg caatgtgtcc ccatccgtct gtcatattga ataatgaagc
     4441 aaaatcctgt cctacttctg cttccatttg cgaagttgcc atagaagatt ccaatgcaac
     4501 attatttggg ttccacagca tcgcatagtt gtaagctaat aaagcaggca tcaatgtttc
     4561 agcattcatt tgtccccagt aacggcctgc tgaatgccat gggatcgatc ctgcgcgtaa
     4621 tcgctgactg acttcatcca agacctctat catatggtcg cgggtggcta gataatctgg
     4681 agtgagctta tctccttcgc taattgcagg catatctgaa ggaatataat tttctctcca
     4741 tccaagatgt tcatctacca tcttgttcaa caacatttta taaactggtc cattttcagc
     4801 tttgtcgcct ataaagacgg ccttgatatc catatctttc atttttcttc cctccatatt
     4861 ttgttggtca atgtcttgca gatacatgtg tttatctgta acacctcttc actatagcgc
     4921 caaaaatgag aaattttttt ttataacatc aaatccacgt agatttttac aaacaaaaat
     4981 aaataaagtc ggatttttct gataattcaa cgcaaaaaag cgaacaattt ttcaataaat
     5041 cagtcttcta aaatagataa cctaaaaaaa gcgaacaaac aaataaaatg actgtccttc
     5101 attcactaaa catattttta tttttttata ttgttaaaaa ttcacaattt attttttaat
     5161 agaattatct gttttccaaa ttttataaca taaaaaacct tttttaacgg catttatatg
     5221 ctttttcaga aattaactga tgatttcaaa aagaaaacat gtcataatat ttcccatatt
     5281 gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa gaatatattg agaaaaatat
     5341 tttaagacag ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa
     5401 gctctccaat ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa
     5461 aaaagaattt gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata
     5521 tttttcagaa catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt
     5581 tttaaaaact tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga
     5641 atgtgaattc atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttactt
     5701 taatgactgc gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg
     5761 tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga
     5821 atcgtttttt gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt
     5881 ttatgataaa gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca
     5941 gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat
     6001 ttacaattat gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga
     6061 agaattcgcc tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca
     6121 gttgattgcg gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat
     6181 aaaagaacta gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc
     6241 gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat
     6301 gccagaaaaa ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac
     6361 aattttttcc agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat
     6421 gcgggctttt gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat
     6481 ctatatcgtc acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt
     6541 aactactttc gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa
     6601 gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc
     6661 ggtccaatat gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc
     6721 agctgtgcaa aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg
     6781 aagatgccca tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt
     6841 tggacatcta ggggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact
     6901 accaacgccg ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc
     6961 ccagctgaaa caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata
     7021 gcattgttct cgcttactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa
     7081 aaatccttga ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt
     7141 cccagtacaa gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta
     7201 cctctcctct tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga
     7261 ctgttcgaat gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa
     7321 ctgactcgat gatatcagca acgactagta cttccaagct taacaaaata taacttgcta
     7381 gcagttttct tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag
     7441 accaatcaaa aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac
     7501 aaatatttaa aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat
     7561 acgctaccta ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg
     7621 ttcgtctatt taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc
     7681 tcccattttt ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta
     7741 aaataaggct tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt
     7801 acccaaattt ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct
     7861 atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc
     7921 tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa
     7981 caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct
     8041 tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt
     8101 atatgatgaa ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg
     8161 gaagacattg gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt
     8221 gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc
     8281 accttttatt cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg
     8341 gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc
     8401 ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct
     8461 tctgttactg agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac
     8521 cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa
     8581 ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca
     8641 gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt
     8701 tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct
     8761 gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat
     8821 ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc
     8881 gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa
     8941 ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca
     9001 ctgactcaag aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat
     9061 gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg
     9121 gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga
     9181 aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg
     9241 acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat
     9301 gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat
     9361 atgtctattg tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt
     9421 atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt
     9481 aaaatgaatg gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct
     9541 ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc
     9601 cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca
     9661 aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct
     9721 tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat
     9781 tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct
     9841 actctagctc gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc
     9901 ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg
     9961 gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaagct
    10021 gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg
    10081 ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
    10141 ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat
    10201 aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc
    10261 tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat
    10321 ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa acacacctct
    10381 ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat
    10441 caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt
    10501 ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa
    10561 tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta
    10621 tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt
    10681 tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc
    10741 aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc
    10801 attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt
    10861 tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg
    10921 aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc
    10981 ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa
    11041 acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat
    11101 tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac
    11161 agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca
    11221 cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg
    11281 attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct
    11341 tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa
    11401 cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt
    11461 ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa
    11521 tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc
    11581 cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa
    11641 aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa
    11701 tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac
    11761 gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat
    11821 taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat
    11881 atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa
    11941 tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa
    12001 aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg
    12061 atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta
    12121 tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg
    12181 catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg
    12241 ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc
    12301 aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg
    12361 aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac
    12421 tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa
    12481 tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa
    12541 aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac
    12601 ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt
    12661 ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa
    12721 ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt
    12781 ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca
    12841 aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg
    12901 ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca
    12961 atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct
    13021 aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt
    13081 ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt tgcttcaatg
    13141 gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag
    13201 aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt
    13261 cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat
    13321 gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat
    13381 gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt
    13441 tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata
    13501 aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa
    13561 atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag
    13621 aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc cccgaatcct
    13681 ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg aatcggtaag
    13741 ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac cataaatgag
    13801 tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg tataaaatgt
    13861 ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta ctgtagatta
    13921 cgcgttcaat cgacgataca gatcaatgat ctcttttgct aaatctgtaa aagctataga
    13981 tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt gatttccttg
    14041 tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag attcctctgc
    14101 atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc
    14161 cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg cttctagatt
    14221 tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt attagttttt
    14281 ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata ttgattacat
    14341 ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata aagcgtactt
    14401 gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc gcatctgaag
    14461 cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt tgcatttttg
    14521 tgactaggag acttgtactc attccagctg aacatacaag catgattgtt ttcttttcca
    14581 ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg tgccaacttt
    14641 aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt tgcatacaca
    14701 ctaaaaaaga aagcgcacac taaaaaagaa agcgcacact aaacacacaa attaattttt
    14761 attacctttt ttcttttaac gtgcacaaaa aaagttgtga cacaaaactt gtcacaactt
    14821 ttttcaaata aacggtattc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa
    14881 aacataagat gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca
    14941 caacctctca ataaacggtg ttcaatcagc tgaccctggg tattagctca aaacgctgga
    15001 ttcacaacct cttttatttc aaataaccag taaatacaat gaacatctac aaaaaacttc
    15061 tatttattta tagatttcgt caggcaaatc caattctctc ttatcaatca aagtctgata
    15121 ccacgaagca gattttttca atgttctgct tcgttcatcg ttcaattcga ttcgaactaa
    15181 tccatatcga ttcttaaatg cattcattgg tgacacacag tcagtgaatg cccataacat
    15241 atagccttcg caatttgcgc cttcttctgt tgctttgatc aagtaatgca aatgctgtcc
    15301 gataaattcg atacggtaat catcttgcac gattcctttc tcatccatat actgttcttc
    15361 atattctctt cccataccgt tttctgttat cagccaaggg atattatgat aattttcttt
    15421 tagatacatg gctatatcat acatgatttt cggatagatt tcccatcccc tggaattatt
    15481 cattttcttc ccaggtaaat caaagacttc ataatatcgt tcaggatgga aaggtgtctc
    15541 ttcattccat tgataacgag gggcgcgaac gcgtttagga taatattgat tgattccaag
    15601 gaaatctact gtatgatgct gaatcgtttc taaatcctct gttgcaggat cgaaataaat
    15661 cgattgtttt tgacacaact ccaggaactc ttctgtgtag tatcctttta ccatcggatc
    15721 aaaaaatact cgattataaa acaaatcata catataagca gctttttggt cttctggaga
    15781 attggatcga gcatagacca tttcaggatt taaaatacag ccaatccttc caccataatt
    15841 tccgctgcgg taaatctcta ctgctttagc cgttgctaaa actttatgat aattccacat
    15901 catccacttt tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat agcaacgcgt
    15961 ttggggaacg attggttcgt tgaatgtaaa ccagtttttt accctatcac cgtaacgatc
    16021 aaaggcaatt tgagcatact tagcataaaa atcaactact tttcttgaag accagccatc
    16081 atatttttcc aaaagaaaag ctgggacttc ataatgttcc aaacaaatca tcggttctac
    16141 gtctgcttcg atcaaagagt cgatcatttc acttatatgc tgagcatatt cttcatctac
    16201 tatacagttc tcataatcag taaaaaaacg agcccaattg atagaagtcc gataatgtgt
    16261 cagatggatg gatttcatca gatccacatc ctgctggtat tgttccataa aattagttgc
    16321 tacggctggg ccatacccat tatgccaaac aaaatgttca tttttatacc agctatctat
    16381 aaacgaatcc tgtccttctt tttttccttt ccaaccttcc gtttgccaag cagaagatgc
    16441 agcaccaaga ataaaatttt ttggaatagt aattttcatg atttcctcct gttttttaga
    16501 tcccatcatt ttccacacga tttgctactt ttacaaatgg cagatagatc aaaatagaaa
    16561 caataataca aacaagttgc gtaataactg caccgatatt tcctgctgtt gccagataag
    16621 cattgacgat cggcggcatt ggccaaggga ccattacaac tgctttcgcc gcaaatccta
    16681 aagcggttgc aaaataacca atcgttccag tgataagagg cgttaagata aacggaatag
    16741 ctaaaattgg attcagcatg atcggcatac caaaaataac tggttcattg atgttgaaga
    16801 tcccaggagc aagggataac ttggtgattt gtttcatatc ttcccgtttt ccaactaaca
    16861 atactgcaat cagtaaaccg atcgtgactc cagacccgcc catactcata tacatatccc
    16921 aaaatggcat agtaataatg ttcggtactt cttttccagc ttcaaaagct tccgtattta
    16981 cagcaattgc cgctaaaagc aacggttccc gtatcggctt caccatttga tttccatgga
    17041 taccaatcac ccaaaagact tgcgcaataa acatcaatag caaaataccc ggtaaccctt
    17101 gtacgacacc ttctaatggg cgttgtacga cattgtaaat gatatcataa aggtacattc
    17161 ctgtaagttc tttgatgata aatcccgcag ccgcgatacc cgatacagtc agaatcgtcg
    17221 ggaataaagc agaaaaacta gcagaaacat tcgttggcac ggtatcaggc attctcagct
    17281 gtagtttctt ctgtttcctc aaccagcaat aaagttcaat agaaaggatg gagaccaaca
    17341 ttcccaaaaa taaccctttc gtatctgtat attgtttagc caatacgttt tctactataa
    17401 cagtctgatt ttcattcact aattcataaa cagttggatt aacagataaa taagacatca
    17461 ctgcgatgat accagggaat acaccatcca ctttattcag cttggctaat tcgatcccta
    17521 tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac atagctgatg gcagatgcga
    17581 taggctttaa ttcttctaaa aaacgcaaga aacttatctg tgctaagcca ttttccgagt
    17641 caaagaccat tgctgaaaac aatgtgccaa aagcacccgt aataattacc ggaatcaatg
    17701 cagcaaatgc gttttttatc accatgatat accttaaatt attgattctc gttgccacgt
    17761 tccctaaaac attggctaat ttatccataa aactcattcc agatcccctc attcctgctt
    17821 cttctccttg acgtcatctc tttttgtcta ttagtcagcg cttacatttc gatgaacaga
    17881 aactatttac tcctcttttt taccaaatat aaaacaaaaa ttcatctgat gtatttatca
    17941 taaaatacat cagatgaact gtcaattagt ttctttttct cctattattt tctgtaattc
    18001 tctccgattt attgccatat ggacagtcat tgcatcaaac gcagcaagtg cattccgcct
    18061 ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc tcgattttca gatgctggat
    18121 atcataattt ttattaaaca aagcaattga ttcattaatg attgggataa tatgcagcaa
    18181 ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta attgaacatg
    18241 ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc gttaattttt tcatttggac
    18301 gatttgattc ttcgttgcat attccgctgc caaacctgcc atcttaggct ccaataaata
    18361 tctcagttca aataagtctc gtatcatccg atcctgatct tcaatcaaag aaaaaccgag
    18421 tgggtcattg gcaatccctc gcttatcact gacaaatgtc cccgctcctt gccgaacttc
    18481 aaggatattc ctggaagcta gagcccgcac agcttctcgc accgtgcttc gccccacctc
    18541 taaataatcg gacaattcat attcattcgg cagtttatct cctacttgca actgcatttc
    18601 ctttaaatat ctaaggatct tgtcagtagt tacttctact agggttttat tttcttccaa
    18661 accacatacc tccttttcat ctgatgtatt ttttcgtaat tattataaca cgtttgaatc
    18721 atctgatgat tttttgtata caaaattgat taatccgttc atagacatac aaacgaagaa
    18781 gatatttacc ataaaaaaga ctaggataaa attcactcct agccttcgat catttttatt
    18841 ttgaacgaca aaaaatatga tgttcaataa cagacgatct tcattcgatt cttgatgtca
    18901 tcttttgttt tttttaatat atggataaaa agtttgatta catcgtttct attctagaat
    18961 ttggtcgatt ctcaattctt cttctggtga gaaatctaaa ttatccatca ttcgaacact
    19021 ttcttgtaac tgacttaacc gacttgctcc gaccagtaca cttgtaacag atttttgtcg
    19081 caaattccat gcaagggcca tttgcgctaa tgattgtcct cgtgatacgg cgattgtttg
    19141 cagtgctttg attttttcca gagttgatcc aacttgttct tctgaaagga atggtatttc
    19201 cttacgatgt gctctagaat cttcaggaat accatgtaga taacgatttg tcagcaaccc
    19261 ttgagcaagc ggactgaacg tgattgcccc tagcccttct tcttctaata ctggagatag
    19321 atcgtcttct agtagtgcac gagaaaacat attgtagcgc atctgatgaa taatgaacgg
    19381 tgcttccttt ctttttaaaa tttcagtcat tttttttgta tcttctccat tatagttgga
    19441 tataccaata tataatgctt ttccttgtct tacaagttgc atcaaagctt ctgctgtttc
    19501 ttccaaaggt gtatcagggt ctgggcgatg atgatagaag atgtcaacat aatctagccc
    19561 cattctttgg agactttggt cacaacttga aatgatggat ttacgagagc cccattcccc
    19621 atatggccct tcccacatat gataacctgc tttgctagaa ataatcagtt catcacggta
    19681 ggcttgaaaa tcttctctaa aaatacgccc aaagtttttc tctgcagctc cagcagggcc
    19741 tccataatta ttcgcaaggt cgaaatgggt aattcccatg tcgaatgcgc ctcttaaaat
    19801 ctctctttga ttatggatag gatcataatc accaaaattt tgccataatc cgagagataa
    19861 aatcggtaat ttcaaacctg aatgaccgga tttacgatat tccatttttt cataacgtgc
    19921 actatctgca atatacaatt tttctcctcc tcatcaaatc tttttaaaac gctttctttt
    19981 ttctattttt agtttactac attttaaaga aaaggagtta atcaaaatga ttttttcgta
    20041 tatttttttg ccatttgttt tttactcgtt gatagatcag aaattgatta cgtgcttgct
    20101 ggattttttt ataacgtttc ggttgttttt tgtaaaattg ctgatgatag ttttctgctg
    20161 gccaaaacgt accggctggt tcgatttttg tgacgattgg ttggtggaaa gttcctgaac
    20221 tgtctagttt ctgttttgat tgttccgcta attctttttg tcgttcgact tgataaaaaa
    20281 tcactggacg atattgcttc cctctatctt gaaattgtcc aaatgtatct gtgggatcag
    20341 taatttgcca atagattgtc agcaactcgc tataacttat ttctcttgtg tcataaatga
    20401 tctccacagc ttccacatga cctgtgtaac caccacttac ttgatcataa tttggttttt
    20461 caacatgtcc gcccgtgtat cctgacaaaa cagataggat cccttttttc gattcaaacg
    20521 gctcgaccat acaccagaaa catccccctg caaatactgc acgttcttga tagtcagcat
    20581 cttgcatggc atgcttggaa aagtcatatt tttgctcctt atcaaatcct tccgtaatca
    20641 ataaataaaa gtccattaca tcaggcgtca gattcattcg gatagctaat ggacgtagtg
    20701 ttgcttctag ttttgataac tgttctttaa cggatatatt ttctcttatg tcattctttg
    20761 tctgaatcaa tacttttcgt tcccaatctc gtgtattcgg attcaaaatc agattataaa
    20821 gtgtttgaat gaatatttgt tcctcatcca tagattctcc tcctttccga agaagacaag
    20881 ctagtgactt tagctgtaat ttatcataaa gtgaggaaat aagcgattac ttcatccccc
    20941 tacagat
//
LOCUS       DASOUD010000043        20945 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.043, whole genome shotgun sequence.
ACCESSION   DASOUD010000043 DASOUD010000000
VERSION     DASOUD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.043"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            591..1280
                     /locus_tag="U4A29_001459"
     CDS             591..1280
                     /locus_tag="U4A29_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0680588.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="U4A29_001460"
     CDS             1267..2475
                     /locus_tag="U4A29_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO0680589.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="U4A29_001461"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="U4A29_001461"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO0680590.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="U4A29_001462"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="U4A29_001462"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO0680591.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="U4A29_001463"
     CDS             complement(6578..7273)
                     /locus_tag="U4A29_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEO0680592.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="U4A29_001464"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="U4A29_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO0680593.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="U4A29_001465"
     CDS             complement(8472..8663)
                     /locus_tag="U4A29_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEO0680594.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="U4A29_001466"
     CDS             complement(8684..9574)
                     /locus_tag="U4A29_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO0680595.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="U4A29_001467"
     CDS             complement(9561..10328)
                     /locus_tag="U4A29_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEO0680596.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="U4A29_001468"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="U4A29_001468"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO0680597.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="U4A29_001469"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="U4A29_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO0680598.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="U4A29_001470"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="U4A29_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO0680599.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="U4A29_001471"
     CDS             14899..15282
                     /locus_tag="U4A29_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680600.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="U4A29_001472"
     CDS             complement(15450..16208)
                     /locus_tag="U4A29_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEO0680601.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="U4A29_001473"
     CDS             complement(16226..17044)
                     /locus_tag="U4A29_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO0680602.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="U4A29_001474"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="U4A29_001474"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO0680603.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="U4A29_001475"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="U4A29_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO0680604.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="U4A29_001476"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="U4A29_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO0680605.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="U4A29_001477"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="U4A29_001477"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO0680606.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5989 a         4261 c         3837 g         6858 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaa
//
LOCUS       DASOUD010000044        20790 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.044, whole genome shotgun sequence.
ACCESSION   DASOUD010000044 DASOUD010000000
VERSION     DASOUD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.044"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(274..1239)
                     /locus_tag="U4A29_001478"
     CDS             complement(274..1239)
                     /locus_tag="U4A29_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEO0680607.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1473..2318
                     /locus_tag="U4A29_001479"
     CDS             1473..2318
                     /locus_tag="U4A29_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO0680608.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2509..3696
                     /locus_tag="U4A29_001480"
     CDS             2509..3696
                     /locus_tag="U4A29_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO0680609.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3702..4058
                     /locus_tag="U4A29_001481"
     CDS             3702..4058
                     /locus_tag="U4A29_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO0680610.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            4060..4398
                     /locus_tag="U4A29_001482"
     CDS             4060..4398
                     /locus_tag="U4A29_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO0680611.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4474..4730
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4793..5827
                     /locus_tag="U4A29_001483"
     CDS             4793..5827
                     /locus_tag="U4A29_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0680612.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5820..6506
                     /locus_tag="U4A29_001484"
     CDS             5820..6506
                     /locus_tag="U4A29_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680613.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6530..7378
                     /locus_tag="U4A29_001485"
     CDS             6530..7378
                     /locus_tag="U4A29_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO0680614.1"
                     /translation="MKKKIFGLAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDNIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7575..8348
                     /gene="sufC"
                     /locus_tag="U4A29_001486"
     CDS             7575..8348
                     /gene="sufC"
                     /locus_tag="U4A29_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO0680615.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFNGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8361..9647
                     /gene="sufD"
                     /locus_tag="U4A29_001487"
     CDS             8361..9647
                     /gene="sufD"
                     /locus_tag="U4A29_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO0680616.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSTVDQLGKNITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRQELVEVIEGKLNG"
     gene            9644..10879
                     /locus_tag="U4A29_001488"
     CDS             9644..10879
                     /locus_tag="U4A29_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO0680617.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHRAIDVQALDCDF
                     FAFSGHKMCGPTGIGALYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10866..11336
                     /locus_tag="U4A29_001489"
     CDS             10866..11336
                     /locus_tag="U4A29_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO0680618.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11341..12732
                     /gene="sufB"
                     /locus_tag="U4A29_001490"
     CDS             11341..12732
                     /gene="sufB"
                     /locus_tag="U4A29_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO0680619.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13801..14466)
                     /locus_tag="U4A29_001491"
     CDS             complement(13801..14466)
                     /locus_tag="U4A29_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0680620.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            14646..16628
                     /locus_tag="U4A29_001492"
     CDS             14646..16628
                     /locus_tag="U4A29_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEO0680621.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            16632..17270
                     /locus_tag="U4A29_001493"
     CDS             16632..17270
                     /locus_tag="U4A29_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680622.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            17298..17726
                     /locus_tag="U4A29_001494"
     CDS             17298..17726
                     /locus_tag="U4A29_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680623.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            18183..19094
                     /locus_tag="U4A29_001495"
     CDS             18183..19094
                     /locus_tag="U4A29_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEO0680624.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            19311..19748
                     /locus_tag="U4A29_001496"
     CDS             19311..19748
                     /locus_tag="U4A29_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680625.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            19843..20727
                     /locus_tag="U4A29_001497"
     CDS             19843..20727
                     /locus_tag="U4A29_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO0680626.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
BASE COUNT         6956 a         3611 c         4333 g         5890 t
ORIGIN      
        1 ctttttcacg acctcttggt aaacggtgtt ccagaagtag ctttttcgga aataagccga
       61 aatttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctggc
      121 cacttctttt acgacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc
      181 ccaaccaatt caaaaacatg aacagctgtt tttgtttggc cgttcttatt acttgatgca
      241 gaacagcttt ttcacaacct attgaatcga ttattaagcg atttttttag aaacaaattt
      301 tccatgtttg ctatgttctt taattaagaa acgtacgtaa tgagcaagaa cgtagaagac
      361 aactacactt gcaaacagac attccgcaac ttttaagaaa tccaatacgc tactatagat
      421 acctaaactg ttgaagtaat gaacagtagt aaatatagct gttgcagaga taacaagcgg
      481 gaatgtaaag gctgcatagc ttggataaaa acgaagcttg aacaaacgtg gaagcataga
      541 cagcacaacg aaatacaata cttgagataa agcgaacaat gctacgacaa gataaaaatt
      601 aggttcagca aagtttttaa gataacctgt taggcaaaga gaaccaggtg ctgcaacgat
      661 cgtaatcagc ggacgtgtcg gatgagccat atttcttacg aaaaagacac gtttgatgat
      721 gatcggcaat aagaacgcat agcagatcaa tgaaatccaa aagaaaattt ttccaataag
      781 cgggaagaag ttcccggcag ttacagtgat gaccccaaat ccaacataaa tgataaacca
      841 gcttggataa attgcttcaa ttgaaagatc agctttgata acatggtgat aattgaaata
      901 aaagacaagg atcacttgga agataacagc gcacagccaa atgaatttga caattggtgc
      961 aatactttca tatgaaacaa aataagtaca aataaccatg agtgacatcg taaaagttgg
     1021 tgaaacggag gcgatgattg gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa
     1081 caatttaaca aaaatgaata tcataagaat catagagata ccgccagtta gattgcctaa
     1141 agcaacatgg tgatagtctt taaacagatt tccaagggaa gctaacccaa ggatcaaccc
     1201 gcagatcggg atcggaataa ttttcaaaaa gttcttcata tataatattc tcctttttat
     1261 ttaatagatt ttagtaccaa gatgaacgaa tacattgtaa aatgaatagg aatgtatgat
     1321 tacgagcaag cagacagctg attgtcttca tctaagttac tagttggatc agcaactgtt
     1381 ttgttgtaca tttctattat agatggtttt ttgtatattt aaatcaaata gtttttatag
     1441 taattatcat aaaaagttat ggaagtggtg ccatgtttaa gttattaacg acttttcgag
     1501 cagtatatga aacaagaaat ttttccagag cagcagaagt actgttttta tctcaaccag
     1561 cagtctcgaa tcaaatcaaa cagttggagc gggaattgaa tatcgatctt tttcaacgta
     1621 acggaagaaa agagatgctt ccaactaagc aagccgactt attatatgaa cggacactgg
     1681 ttttgttaga agaatgggaa gaaactcagc aaaaattgct caatgaacaa aatgaagaag
     1741 aagtatgtcg aatcgttgct tcccacacct ttgctgtcta tcctttgcct cagttgatga
     1801 aacatctgat caaaaggttt cctaaagtgc attttgagat cgaaatcgcg aattctcatg
     1861 aatcattaga acaagtagcg aaacatgaat ccgattttgg ttttatcgaa aagccattag
     1921 agacttccgg cgtacaaaga tactctttga tggaagacca gctagtcatc gctggggacc
     1981 caaaaagcaa attgtggctt gtaagagaag caaactctgg tgtttttcat tacacgcagc
     2041 gttattttga agagatgaat atccaagata agaagatgat cgtgaaaagt aacgatgtaa
     2101 ttgttgcgtt gctgaaggaa ggaatcggtc gctcgatttt gtctaagcgc tctgttccag
     2161 aaaatgtgcc aatgactccg ttgaatttcc atcgttattt ttatttcatc caacgaacca
     2221 atctggtttc tgatcagctg ctagagattg ccgaagagat ccttcgatac aatgaaagca
     2281 cagaaacttc tcacagcgag gagaatctca gttggtagat aattgcatgt atcaaaaaaa
     2341 gttctatgat acactaaaga taagcagtag gcttttgtac tgcatttgct caagcaactg
     2401 cacaaacgga aagttaattt ttcccaaatg tctatggaaa gactttccat tatctaactg
     2461 gaccgctata ataatcagca gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa
     2521 ttaaaagctt cgaacgataa tcgtattgat tatggcgtga ttttaccagt ctttctgtta
     2581 tgtttgattg gtctgctctc attatatgta gcacttagcc atgatccgaa caatccagca
     2641 gtcttgaaag gcgtagggat gcaagttgtc tggtatttag ttggagcggc agcaatcgtt
     2701 gtcatcatgc atttgaattc caaatggatc tggaaactga ctccttactt ttatggtttg
     2761 gggttagtgg taatgggact cttgctcaaa ttctatgatc agaatttagc tgcttcaaca
     2821 gggtctaaaa actggtttcg attcggtgcg tttactttcc agccagcaga gttgatgaag
     2881 attgcgtaca tcttgatgat ggcactgatc gtaactaaac ataatacgca agtaaaagaa
     2941 cggacgatga aatcggactt ttggctgatt gggaagctct tgatcgtgac aataccggtt
     3001 ttagcattga tcatggccca agatgacttt ggaacgatgc ttgtctttct tgcgatcttt
     3061 ggcgggattt tcttgatgtc tggaatctct tggcgcatca tcgcacctgt agtaatcttg
     3121 gcagtagtcg ttggagcagg aacgatcttc ttggttacga ctgaaggcgg aagagacttg
     3181 ctttacaaag ttggcttcaa atcttatcag tttgcacgta ttgattcatg gctggaccct
     3241 ttccatgata cgtcaggaat gagttatcag ccggcccaag gacttttggc aattggtacc
     3301 ggtggcttgt ttggtaaagg attcaacgta agcaatattt atgtgcctgt tcgtgagtcc
     3361 gatatgattt ttaccgttgt cggtgaaaac ttcggcttta tcggtggtgc atttgtcatt
     3421 ttcttgtatt ttattttaat ctatcgtatg attcgggtgt gtttcgatac gaataatgaa
     3481 ttttacgcct atatcgcaag cggtttgatc atgatgctgc tgttccatgt ctttgagaat
     3541 attggagcaa atatcggatt acttccttta acagggatcc cattgccttt cattagtcag
     3601 ggaggatctt cgattttagg gaacatgatc ggtatcgggt tgatattatc gatgagatac
     3661 caaaatgaag cacctgcaag atcttcaagg aggtaagaaa attgtacacc ttttactggt
     3721 acccgaaatg ttcaacatgc aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt
     3781 atcaaactgt tgatatgatc aaagaagtac cgaaggcctc aacgattgag acttggctag
     3841 aaacgagtca agtaccaatc cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac
     3901 ttggactgaa agatcaagta ggcgagttta cggcttcaaa agcgagccaa gtcttatcga
     3961 cagatggtat gctgatcaag cggccactga tcttacgaga ggaccagctt gttgcaattg
     4021 gattcaatga aaaaatttat gaaggagaac tgatataaaa tggcaggcaa gcatttgaag
     4081 aaaaaggaca acctttggat cttatttaat ggaaaagaat actgtgttgg tttgacaaat
     4141 gaagcgcaag aagaacttgg agacatcacg tttgccaatc tgccaaaagc aggacaatcg
     4201 ctaaaaaaag atgaacctct gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc
     4261 agcccgctaa cgggtacagt ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc
     4321 ttgaatgatt cagatgaaat gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa
     4381 gaatttgacg aattataaaa tcagaaaatc acttgaaaaa agttgacact gattcttcca
     4441 gttgctataa tcataagcaa tcctatatga aacgagaaag ctttgaaaag aagagtacat
     4501 gtcacggacg tttgaagaga gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa
     4561 gatggtctta gagcagagta agcgagcgta agcaaactta ctacgggagt gcccgttaca
     4621 gcgcgacagt atacggaaag aagctaagtc caagtactgg aagaggctgt tattgcgaag
     4681 taacggtaaa tcaaggtggt aacacggaag ttcaagcttt cgtccttttg cttaaggcaa
     4741 gagggcgaag gcttttttgt ttgaaaaatc ataagagaaa gatgtgaatg gcatgtcatt
     4801 aatccaacta acgaatgtca aaaagacatt caatgggaaa aacggaaaca tccaagccgt
     4861 caatgacgtt tcactaaacg ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg
     4921 cgcaggaaaa agtacattag tccgtctcct gaatggactt gagcttccca cgagcggcga
     4981 agtgatcgta aacgatcaag atattacaaa attaaaaaac aaagaattac gtacattccg
     5041 aaagaaaatc ggtatgatct tccagcattt caacctgtta tggtcaagaa ctgtacttga
     5101 aaatatccag ctgccgctgg aattagctgg cgtaccaaag agcaaacgta aagaacgggc
     5161 agaagaactg ttgcgtttag tcgggttaga aggacgcgga caagcatatc cttcacaact
     5221 ttccggcggg cagaaacaac gcgtcggtat cgcacgagca ttggcaaatg atcctgagat
     5281 cctgctttgt gatgaggcta ctagcgcatt ggacccgcaa acaacagaag aagtattaga
     5341 cttattgtta gcgatcaata agaaattgaa tttgaccatc gtcttgatca cacatgaaat
     5401 gaatgtgatc cgcaaaatct gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga
     5461 agaaggagac gtattgactg tcttcagaca accgaaaaaa gaagtaacca aacgctttgt
     5521 acaacaagac atcgaaccag aagctgattc gacagaacta ttagctgaac tcatcaaaga
     5581 aaatccaagc ggccgtttgg caactttgac ctttagcgaa agaaatgcca acgagccagt
     5641 catttcccaa gcgattcgac gctataacat cgatgtcaac gttgtgtatg gaaagatcaa
     5701 acaagtgaaa gaaggttcct ttggttcatt gactgtcttg atgactggtg aaaccaaaga
     5761 attagatcaa gcagaagtgt tcatcaaaga acaaggagta ggaattgagg tgatccatcg
     5821 tggataaaag cttttcagaa acatatctag attttaaaca aatcaacccg gaaacgatga
     5881 atcaggctat ccttgataca ctatatatga cattaatctc aatggtctta gtgacgatca
     5941 tcggattagt tttgggattg atcctttact ctattggacg caaaaacaaa ccgtatgctc
     6001 gaatcattta tggaatcgta tcgatcatca gtaatatttt ccggtcaacg ccatttatga
     6061 tcctgatggt tttgatcatt ccatttacta aagcaatcgt aggcactatg ctaggtgcgc
     6121 aagcagccat tccagctctt gttttatccg cagcaccatt ttatgcccgt ctagtagaga
     6181 ttgcttttcg tgaagtcagc cctggtgtac tggaagcagc agatgcaatg ggagcaagtt
     6241 actgggaaat catctggaag gtcttgatac ctgaaagtat tcccgcattg atttctggat
     6301 taacagttac aacgatctcg atgatcggat ttactgcgat ggccggcgcg atcggcgcag
     6361 gtggacttgg aggtcttgct tggcaggaag gctaccagcg tggtaatctg acagtgacat
     6421 tcgttgcgac tttaattatt ttactaatcg tatttatcgt tcaaggaatc ggtgatttct
     6481 taactaagaa aacagacaaa agataatagg atataggggg atgtaggata tgaaaaagaa
     6541 aatttttgga ttagcagcag cgcttttgct gactgtagga ttagcagcat gcggtaacaa
     6601 cagcgacagc aaagattcag caaataaagc agacgacacg acattgaaag tcggcgcttc
     6661 accgacaccg cacgcagaga ttttggaaca tgtgaaacca ttattgaaag atgaaggaat
     6721 cgatcttgaa atcgtcaaat tcgatgacta tgtattaccg aaccaatctt tggaagaagg
     6781 cgacattgat gccaattact tccagcatat tccttatttg aacaaagaaa tcaaagaaaa
     6841 aggatatgac tttgtcaatg ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa
     6901 aaaagtcaaa gatgtttccg aattaaaaga tggtgcaaca atcattactt caaattctga
     6961 atcagactgg ggacgcatca tcacaattct acaagatgct ggcttagtca aagtcaaaga
     7021 tggagtagat ctggaaacag caacattcga taatattgaa gaaaacccta agaacttgaa
     7081 gttcaaccat acgatcgacc catcactttt agcttctacg taccaaaatg atgaaggtga
     7141 cctagttgca atcaacgcaa acttcgctta cggcgcaggg ttgaatccat tagaagatgc
     7201 cgtattgctt gaaaaggaca actctcctta cgtgaatatt attgcgactc gtaaaggtga
     7261 tgaagacagt gacaaaatca aaaaactgat tgaagtgctt caccgcaaag aagtccaaga
     7321 atggatcaca gacaaatggg gcggctctgt taaaccagta aaggctgatg caaaataagg
     7381 tatggaacaa aaaagatgag aaataagtct gtaatggctt atttctcatc ttttttatat
     7441 ctattctaat ttagaaatca aaattgttta ataataaata aaagatattt gattttagta
     7501 aggaaggtca ttgcgcttca ttctcaatta gtctaaaatg agtgagaacg aaagagaatt
     7561 tgggggtata atgcatgtct gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa
     7621 aagaaatcct taaaggcgta aacctaacaa tgaaaaccgg agaaatccat gccatcatgg
     7681 gacctaacgg cacaggaaaa tctaccttat ctgccgctat catgggcaat ccaaactatg
     7741 aagtaacaga aggcgaaatt ttgtttaacg ggaaaaatgt tttagacttg gaagtagatg
     7801 aacgtgcgcg acttggctta tttttagcta tgcaatatcc aagtgagata ccaggtatca
     7861 cgaatgcgga attcatgcgt gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt
     7921 ctgttatggc atttttgaaa aagttagatg aaaaaatggc tttactaaat atgccagaag
     7981 aaatggctga acgttatctg aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga
     8041 tcctgcaatt gttgatgtta gaaccaacat ttgcaatttt agacgaaatc gattcaggtc
     8101 tggatatcga tgctttgaaa gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt
     8161 tcggtgcatt gatcatcacc cactatcaac gattattgaa ctatatcaca cctgatgtcg
     8221 ttcacatcat gatggaaggc cgagtagtca tgacagggaa tgctgaccta gctaaacgtc
     8281 tggaagcaga aggctacgct ggtatcagta aagaattagg aatcgactat aaagaagaag
     8341 aagcgtaagg gggagcaaga atgaaagaaa aaaactggct cgattatctt gacgcggtaa
     8401 atgatttttc tttaagtaaa ggagaacctg attggatgag aaccttccgt caagacgcac
     8461 tagcgaaagc agatgaactg ccattgccgc atatcgatcg ggtgaaattc catcgctggt
     8521 cgttatttga tgtgaaagaa acacaaacca tcagtgaaac aggcacgatt ccggcatttg
     8581 atgcaatgaa agacaatcct gtgcttgtcc aacaaggttc atggacgatt tttgaacaac
     8641 tgcctgtgga attagcagaa aaaggagtca tttttaccga cttgttcact gcaatgatcg
     8701 aatatccaga actagtccaa gaatattata tgaaaaaagc agtaaacatg aacgaagacc
     8761 aattgactgc actccatgta gcttttatga acagcggtat tttcctgtat gtgcctaaaa
     8821 atgtcgtgat cgatgaacca ctcgagtcct tattcattca agatggcgca tccgatgagc
     8881 acttcttcaa gcatgtgttg atcgtggcag atgaacatag tgaattttct tatctggaac
     8941 gtttccagac aaccaaagaa caagtagcaa aatcatcagg taatatcatc gtggaagtca
     9001 tcgctaaagc tggttcaaaa atcaaatatt caaccgtaga tcaattagga aaaaatatta
     9061 cttcttatat gaatcgccgt gggcacattt tacgagatgc ttctgtcgac tgggcaattg
     9121 gtgtcatgaa tgacggtcat gtgatcgctg actttgactc agatttagca ggagaaggtg
     9181 cacacgcaga agtcaaaatc gtggcaatca gctcgggcag acaaatccaa ggaatcgata
     9241 cgcgagtaac caataaagcc cctcatacga tcgggcatat tttgcaacac ggagtgatcc
     9301 gagaaaaagg aacattgacc tttaatggta tcggccacat cttgaaaggg gcaaaaggtg
     9361 cagatgccca acaagaaagt cgtgtcttga tgctttctga taaagcacgt ggtgatgcga
     9421 atcctattct tttgatcgat gaaaacgaag taactgctgg acatgctgcc agtgtaggac
     9481 gagttgatcc agaagaaatg tactatctga tgagtcgagg attgcataaa gaagaagcag
     9541 agcgcttagt gattcgagga ttcctcggtt ctgtcttaac agcaatccct gtagaacaag
     9601 ttcggcaaga attagtcgaa gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa
     9661 gctacgcaat gattttccta tcttatttca agacgtcaat gatgaacctt tagtttatct
     9721 ggataatgca gcgacgaccc aaaagccaaa acaagtactc caggcattag cgcactatta
     9781 cgaacacgat aacgccaatg ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca
     9841 tgattatgaa caagcaagag aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga
     9901 agtgctgttt actcgaggta cgacgacaag cttgaattgg gtagccagaa gcttcggcga
     9961 ccgctatatc aaagcaggag acgagatagt gctctcttac atggaacatc attctaatgt
    10021 tatcccgtgg cagcagttgg caaaacgaac aggtgctgtc ttgaaatata tcgaactaac
    10081 agaagaaggc ttcctggata tggaggatgc agcgaaaaag attacggaca aaaccaaaat
    10141 cgtctccatt gctcatgtat ccaatgtctt aggtgtcatc aatcctgttc aagaattagc
    10201 tgaacttgtc catgaaaacg gcggggtcat cgtagtagat ggtgctcaat ctgccccgca
    10261 cagggccatt gatgtccaag cactagactg tgatttcttt gctttcagcg gacacaaaat
    10321 gtgcggacca acaggtattg gtgctttata tggtaaacga aaatggttag aagaaatgga
    10381 acctgttgaa tttggtggcg aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa
    10441 agaactacct tggaaattcg aagcagggac accaaatatc gccggcgcta tcgcattagg
    10501 ccatgcaatc gattatttgc aagaactcgg catggacaat atccatcgat atgaagaaga
    10561 actagccgct tatgtgttgc caaaattaca agcaattgat ggattgactg tttatggtcc
    10621 acaagacccg aattttcgga caggagtcgt tgcattcaat ttggatgggt tacatcccca
    10681 tgatgtcgcg acagctttag acatggaagg ggtagccgtc agagcaggtc atcattgtgc
    10741 gcaaccgcta ttgaagtacc ttgaagtatc agcgactgct cgcgcgagct tttatttcta
    10801 caacacaacg caagacgccg accgtttagt tgaagcaatc atggcaacaa aggagttttt
    10861 ccagcatggg actatctaaa ttagacaatt tataccgtca agtgatcttg gatcattctt
    10921 cccatccgca tcatcgtgga agcttgactg actcgacaag taagatcgaa ttgaacaacc
    10981 ccacatgtgg agatgtgatc gaattgcaag tcgatgtaaa agacggaatc atcaaaaata
    11041 tcgcctttga cggcagtggc tgctccatca gtacggcaag tgcctcgatg atgacagacg
    11101 ccgtcatcgg aaaatcaata gaagaagcag aacatttaac tgtagacttt ctacagcttg
    11161 tccaaggaaa agaagtcaaa gatcccgacc aattaggcga tgctgctatg cttggtggtg
    11221 tagctaaatt tccagctcgg atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag
    11281 caatggaaaa cgatggtacc gcagctttcc atcattacca caaggaagag gagtgagaac
    11341 atgggcgtac cagaattaga agaatataaa ttcgggtttc acgatgatgt cgaaccggtt
    11401 ttcagtacag gagaaggctt gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc
    11461 gaaccagaat ggatgcttga attccgtctg aaatctctag aaacatttaa caaaatgcca
    11521 atgcaaacat ggggccccga cctttcagac atcgatttcg atgccatcaa atattaccaa
    11581 aaaccaagtg acaaacccgc tcgtgactgg gaagacgtac cagaaaaaat caaagataca
    11641 tttgaaaaga ttggtatccc agaagcagaa cgagcttatc tagccggtgc ttccgcacaa
    11701 tatgaatcag aagtcgtgta ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc
    11761 tttactgaca ctgactctgc tttgaaagaa taccctgaat tattcaaaga gtacttttca
    11821 aaacttgtac cgccaactga caacaaatta gcagcgctga attcagccgt atggtcaggt
    11881 ggcaccttta tttacgtgcc aaaaggcgtc aaagtcgatg ttccattaca aacttacttc
    11941 cggatcaatg cggaaaacac gggacaattc gaacgtacgc tgatcatcgt tgacgaagga
    12001 gcaagcatcc actatgtcga aggttgtacg gcacctactt attcaagtaa tagtctgcat
    12061 gccgccatcg tagaaatctt tacaagaaaa gatgcgtatt gccgttacac aacgatccaa
    12121 aactggtcag acaatgtgta caacttagta accaaacgag caaaagcata cgaaggcgca
    12181 accgttgaat ggattgacgg aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt
    12241 tacttagatg gaaaaggcgc acgaggcacc atgctctcca tcgcctttgc aggagcaaac
    12301 caaatccaag atacgggtgc aaaaatgatc cacaatgcac caaatacatc tagctctatc
    12361 gtctctaaat caatctcaaa agacggcgga gaagtaaact atcgaggaca agtaaccttc
    12421 gccaaaaaca gtgcgggttc gatctctcat atcgaatgtg acacaatcat catggacgaa
    12481 tggtcgaaat ccgatacgat cccatttaat gagatccaca acagccaagt ctctttagaa
    12541 cacgaagcca aagtctcaaa aatctcagaa gaacaactct actatctgat gagccgaggc
    12601 ctgaccgaat ctgaagcaac tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa
    12661 gaacttccaa tggaatatgc agtcgaactc aaccgattga tcagttatga gatggaaggg
    12721 tctgtggggt aatacttaaa taaaggctag gaacctgata acatcaaggt tcctagcctt
    12781 tttataataa aatgaagcga aaataatttc ttttaaagag ccgagattat cagctatata
    12841 agagtgaatt tgatgcaaaa aacagagccg aaacagtctt ggtgttcgta gctctgctta
    12901 tatgtttcga atttaagaca aagagttggc aaaagatgtt gataaggaag ataagataac
    12961 tgtgttatat aaactaggta actaactgta acaagctaat tttaagagat tcttattaga
    13021 agacgtttag gaatataact aagaaaatga ttatacaggt aattgtcatt tcaataatgt
    13081 tttctaattt tcgcgtcttt gttttaagaa aaaggccaga atcagttgga aaaaagttcg
    13141 tacgatagtg aagaggggca aaatttccta ctaatattcc cactaacaag agataaaacg
    13201 gccaggttgg tgtgattcct ctataaaaaa atgcccatgc taccgaaatc ccatagatta
    13261 acaactgaac aatttttaca atgaaaaaat tgttctgcca ttactcaaag tctcttcctc
    13321 cttgtatcgt ttatttgttt cattttatca ttatgatagg cgcttgaaca atccattttg
    13381 tattttattg agtactcttg gataaactac cagcgattat catatttatt tatgttactg
    13441 taagccatca tataaaagta aatgagcaga tcatagaaat ataaaaaaga actaaaattg
    13501 atctaaatta tgtgcagctt gactacgtac caatctatat tacttactag attggtcgtt
    13561 aatctattcg gatcgaatga gcaaaatatt tttgtgaatg tccatccttg attatgattc
    13621 caaaaagaat cattgggagg ctgtggcgta aatattatac tataagccag aaatacctat
    13681 cattctgaac ttttttattt aggaactttc atctcaaggc tttttcaatt aatttgtacc
    13741 cctgtttttt ggataagatc atgtgcattg attttttcta tctctacgtt catctcctac
    13801 ttaatgccta tctggctcct gagggcttct ttttaaggcg tcgtcatagt cttggctgag
    13861 gatttcttgg taatctgagg gaatggcttc ccaagtgagc aggcgaacgt tcatgacgtg
    13921 ttttcctttg tgtttgatta cagcatggtt tttctctttt tctacctctc cagcgtgaaa
    13981 agacagccat gtaccatggc caaagatatc attacatacc caacgttcga tggaattgtc
    14041 cacgtatcct acagggagtg tggtatagcc gatttgtggt ttcaaaaaag gattgaatcg
    14101 atcgatcgtt tcatttttgt attgataatt gataagaaaa agatagacca aacaaaagac
    14161 gagcagaaag aaagatagtg tctttttgag tgagaagggt tgttttgatg aataagattt
    14221 cttgttaaca agaggttcat ctggcaatgt tggttctatt gattcggtag ctgattcaat
    14281 agaaggaaaa tcaatcggca gacctaaagt atgatgtaaa agtgacagca tttcaaggtc
    14341 tggcattgta cgagaatttt cccaactaga aatcgtttta tcggaaacac caattttttc
    14401 gcctaattct ttttgcgtaa gcttacaatt tctgcggtaa gttcgtattt tttctccggt
    14461 gttcatcttc ttctcctttc gattctacag tatatagatt tattctacat gacctataca
    14521 cttgatagat acgttttttt gatgaaaaac ggtatgattt tatcaaagaa tcatttaagt
    14581 aagggagagt ggtgataatc aaaaaaaagt tcacagtttt actcacaatc atagtgaccg
    14641 gtatcatgag tggttttgcc ttggttagta taaataaaca ggtactctat cggacaaatg
    14701 aatttttcca tcaaacagat acttatactc cgattcgatt gccacaacat tttattgggc
    14761 aagaagaata tgagcagcta atcatggtat ttgaaaatgt ctcatcagaa actgagcttt
    14821 cttatatgaa aaaaacagta aatgaatcca atgaagtaga tacatcttgg aacttcaaga
    14881 tacagccaaa gcttgaaatg acattgtatt atcaagggga tgtcaaatct tctcacgaga
    14941 aggtacccta ttctaatacc tcctttcgcg taacgtccat tgaagaatcg atcaaaataa
    15001 aagattttga aggtgaattt ttcttgatga cgagagaaaa agaagcctat gaacgggtca
    15061 tttcactatt cactgatcga ttcaatcaag catttcacac tgattatgcc actaatattt
    15121 ttgaagactt tgaaaatagc tattcgtatt cggttatttc aaatacagac gtatataatt
    15181 atcgagtgta tttaactatt ggattgattc ttttattcct tttgttatca tcctttttct
    15241 tattgatgaa taaagaaatc agtttattgt tgaataatga cttttcatta catcaagtat
    15301 tgtggtgttt attggggaaa aagaaaatca ttattacatt cataattgca ggaatcattg
    15361 gtggttttat ttttatgtat tataaggagc aatttcttta tttgttccag atgtacgttt
    15421 tcttgttcag cggaatatat ggtatctctt tgatttctct tttattagtt gaaaaaatcg
    15481 cttcgttaag agaagcgttc aactatatcc aatacattct ttttacattt ggattgttgt
    15541 tttctcttct tttgaccgtt gccaatgtcg aattagcaac tatcgttttt tcttcttcac
    15601 agttagctgt ttcccatgaa ccaccaaaag aaatcgctgg tgaggactat catgtatttt
    15661 atccagtaac catcgggaaa aaccacgtgg attttattta ttccaatcgt tttgcatcag
    15721 cagcagatca ggaattatat gaaactttga ataaaaacgg aagtatatta gtcaatgtat
    15781 cagattactt gaatgaagaa aatgaacagt ttggcagagg gataaaaata aatctgaatt
    15841 atcttaagaa atttcctttg attgatgtct ctggaaggtt gatccaaata accgaaaaag
    15901 aaacgcaccc tgttatattg atccctgaac gatatagaca tacagattta aaaaatgacc
    15961 tggcgcaaat aacagaatat tatcaagaaa tcagtgaaaa agaaccgaaa tttctattta
    16021 taaaaaacaa tcaacccatt tatacattta ttccttctat cccctggatc gaacattatc
    16081 cggttgttga aatactgaca ttgaaaaaca gtatgtattt ggaaagaaat attctcagcg
    16141 gagaaatcta tccaccgtta aagatcaaga taggctcttt gtcgaaagaa aggttatttg
    16201 agctgattga agaaagtcag ttacgtgata acttgcagtt atcatttccg tatactcaga
    16261 ctgaagagat agctgtcaaa gtgttaagca gatcatttca ttacttgata cagatatttt
    16321 tactcacttt cttttctttc ttcttgttga gctatgtcat gttatgcata tattttgtct
    16381 acaattgtcg gaaaatcgct atctttcgtt caagtggtta ttcgctcttt gaaacgtata
    16441 aagatttctt catgatgaac ctcatcaaat ggggaacgac aagtgtaatc ttcctttttc
    16501 ttatagaaag agagcctaaa tatcttttta atattttttt ctttagcttc attgatttta
    16561 ttctttcagt tgtttttatt ttgtctacag aaaagaaaag tcagttattg ctgatgaatg
    16621 gaggttaaca attggagccg ttgatagtga taaaaggtgc gaaaaaaatc attcgaaaca
    16681 aaacgattct tcatttggat cgtttcactg cggatgctgg ggatcttgtg actgttgttg
    16741 gagaaagtgg agcaggtaaa agtacgttat tgaatatatt aggattgaat gatcagttca
    16801 gttcaggaca attctatttg ttcgatcaag agacgaaaca attaactggg agagagaagc
    16861 gcttgataaa aagaaatacc atatcttttc tttttcaaga ttttgggttg attgaatatg
    16921 aaacaatcga ttttaattta gatattggat tgaagttttc gaatttaaat gtttcagata
    16981 agcgaacagc aaagtatgag gcattgaaaa aagtaaatat aaacaagaag ctaagtacgc
    17041 ctatctacac attatctggt ggtgagaagc aaagaatcgc ctgtgcgaga gtcttattga
    17101 agaatagcaa aatcatttta gccgatgaac cgactgggtc attggataaa agaaataagg
    17161 ataaagtagt tgaaatctta tcgcaacaag cttcccaagg tacgttagtt atcgtagtca
    17221 cgcatgatcc atatctgacc aaaataggaa acaagaagat catgctttaa aaatcaaaaa
    17281 ggaaaggatg tcgttttatg aaaaaagcag ttattatttg tgcactaggg ataggttttg
    17341 ttggaggaaa cgctatgtca tctgaagtgg aagcaaattc agttcacggt gaaatctatt
    17401 acaaatcttt aaagccagga gagattcatt ttgatgctaa aggtaatatt attaagtcaa
    17461 atttaccagt agaccgggaa atttctagaa cagcaattaa tcatagctct ggaagatggg
    17521 tttattatag tgatacaact tgtacgagaa aaacaggtca ttctaaccac tatagtcgtg
    17581 tctataatcg tcacggtgca aaagcgaaag ttggaaactc tattcgatca gcaaatgcaa
    17641 caaaaggagt atgggcctat gcaagtgcga caggaaatag aactgacaca ttctcatgtt
    17701 ggtacaatcc aaccggttgg tattaagtat ccaaaagttt atataaaata gaaaaaacat
    17761 ataatgaaac agcgttaaaa gttatttttg gtagttttaa tatattcatt tttagttgta
    17821 taaaaatata tcataataaa aacaacaaaa aaatgtctgg aaaatcgaac ataaaagtgt
    17881 ctgatttgaa tatcccagca gcggaagaag aaaaacaaaa tcgataacat ttgttctaag
    17941 aattagttac gttaaatacg gaacgcaaga tagacttgaa aaacggtaac aaaagaagta
    18001 gtatattaag gtgtgatgtg ctacttgaca tttattctgc tgagcgaact ttctggattg
    18061 tgtagcctta tatctattca ggacgtatcg agcagattag taaaggtgtt cttgatgatt
    18121 ttcatcttga acacctttgt ttttagcaat tcgcaaaaaa cgaaaagtag gagaattgat
    18181 aaatgaaaaa aagattttta gcacttgcca tcgtactagg cactggctta ttgtcaggct
    18241 gtacgaatgc cggtgaaaaa acagccgtta gttataaagg cggtacaatc agcgaacaag
    18301 aagtaatgga tagcttgaaa aaaatgcaag gagcagattc cgctgtgcaa cagttgatcg
    18361 tttatcaagt atttgaagat aaatatggag atgacgtatc aaccaaagaa atcgattctc
    18421 aatatgatca aacaaagaaa caattgggtg attcatttga ttcacaattg aaatctgcag
    18481 gttacacaga acaaacattc aaagacagta tcaaacaaag tctagccttt caagaaggtc
    18541 taaagaaaca tatcaaattg accgatgaag atttaaaaac ggcttgggaa tctttccatc
    18601 cagaagtgga agctcaaatt atccaagtag caagtgaaga tgatgcaaaa gatgtgaaga
    18661 aagctgctga taagggagac gatttctcta aattagccaa agacaagtca actgatacta
    18721 cgacaaaaga agatggcgga aaagtgaaat ttgattccac tactacaacg gttccggcag
    18781 aagtcaaaga agcagccttt aagttgaaag atggtcaagt ttctgatgtg atcacttcta
    18841 ccaatgcttc aacttatact acggaatatt atgtggttaa aatggtgaaa aaccaaaaca
    18901 aaggcaacga catggataaa tacaaaaaag aattgaaaga aattgcaaca gacacaaaat
    18961 taagtgacag taccttccag aataaagtaa ttggcgaagt attgaaagac gctaatgtaa
    19021 aaatcaaaga caaagacttt gaaaatgttt tgagtacatt cacttcagat tctagtacag
    19081 caagtagtaa ataagcggta taaataaaaa atatgaacta tttaaatcaa taaaactgtt
    19141 catctaaatc tttttattag aactaaatca acagtagttt aattactact tgatttagtt
    19201 ctttttattt taaaaatttt ttttacttag aatccagcga ccctagatat ttttctgtat
    19261 aatcttaaat aaagaaaaca atatcaaaaa atgcacgtag aagtagataa atggagtggt
    19321 tttgcaaatt tcttttattt ctagaagtta ttcacgtata cggatttgtt tttctcacaa
    19381 ataaaaaggc aggaagtaca atggatcatt tgtatataaa atatattcat caattttccg
    19441 ttttggtgtt aggcacacta attatagcgg ggattgtcgc atttattatt caaggccgtg
    19501 acatattaac agtacatcaa gagtttttca gttatgcatt tggtattcct ctgatcgcta
    19561 taaggtctga taacaaaaag tggaagaaac gctattacat tctcttgcta tttttgtgtt
    19621 tttatctgcc acctaagatt tttactttat taggtataca atgcctatca ttttctttta
    19681 tttcctatat cattgtttta attttcctca gtttcatagt atggatgttt tatcttgtca
    19741 aaaaataaat gaatgtttca tgaacagtac ttcatcacag cctaattttt ttctttatgc
    19801 tatcgttata ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa agttttatta
    19861 gtggtaaacc ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt agcgatagca
    19921 aaattggaat cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc aggggatgca
    19981 aaaaacttta ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt catgggcgga
    20041 gatggaacag tcaacgaagg aatcagtgga atcgctgaac aagaacatcg gccaaatttc
    20101 ggatttttcc cattgggtac ggtgaatgac ttggctcgag cattagggat accgctagaa
    20161 cctgaggaag cgatcaatca tttttcgatt gaatcagtca agccattgga tattggaaaa
    20221 ataaatgacg actattttat gaatgtcgtc gcaattggct ctatccctga atcaatcaat
    20281 gatgtagatg cggagaagaa gacgaaattt ggaaaactag cttatttcat gtcaggaatc
    20341 aaacagttag cttccacaca atcctactcg tttcacgtag aagttgatgg aaagaaggaa
    20401 gaaattgaga gcagtacctt gctgatagga ctaactaact cagtcggcgg ttttgaaaca
    20461 ttacttccaa atgcaaaagt ggatgatggt aagctccatt tggtttattt aaaagattct
    20521 tctttgatcg atacgcttaa aacattgcca gacctattaa aaggagtaga tgaatctacg
    20581 aaaaatctag cctaccttac ttgtgagcaa ttagttgtgt cattagctga tgaggaagaa
    20641 ttgacgacga atgttgatgg agatgaaggg gataaattac ctgtgacaat caaagtattg
    20701 ccctcacatt tacaggttta ttgttaaaat ggaggttgtg accgaggagt tggcgggaga
    20761 acaccgcttg ttcgggataa gggactgtga
//
LOCUS       DASOUD010000045        20762 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.045, whole genome shotgun sequence.
ACCESSION   DASOUD010000045 DASOUD010000000
VERSION     DASOUD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.045"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(300..1610)
                     /locus_tag="U4A29_001498"
     CDS             complement(300..1610)
                     /locus_tag="U4A29_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEO0680627.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1622..3040)
                     /locus_tag="U4A29_001499"
     CDS             complement(1622..3040)
                     /locus_tag="U4A29_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO0680628.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3280..4848)
                     /locus_tag="U4A29_001500"
     CDS             complement(3280..4848)
                     /locus_tag="U4A29_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEO0680629.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4852..5262)
                     /locus_tag="U4A29_001501"
     CDS             complement(4852..5262)
                     /locus_tag="U4A29_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEO0680630.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5259..6182)
                     /locus_tag="U4A29_001502"
     CDS             complement(5259..6182)
                     /locus_tag="U4A29_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEO0680631.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6179..7057)
                     /locus_tag="U4A29_001503"
     CDS             complement(6179..7057)
                     /locus_tag="U4A29_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEO0680632.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7044..8381)
                     /locus_tag="U4A29_001504"
     CDS             complement(7044..8381)
                     /locus_tag="U4A29_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEO0680633.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8384..9487)
                     /locus_tag="U4A29_001505"
     CDS             complement(8384..9487)
                     /locus_tag="U4A29_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO0680634.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9561..11069)
                     /locus_tag="U4A29_001506"
     CDS             complement(9561..11069)
                     /locus_tag="U4A29_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO0680635.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11035..11727)
                     /locus_tag="U4A29_001507"
     CDS             complement(11035..11727)
                     /locus_tag="U4A29_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEO0680636.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11749..12723)
                     /locus_tag="U4A29_001508"
     CDS             complement(11749..12723)
                     /locus_tag="U4A29_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0680637.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13023..13895
                     /locus_tag="U4A29_001509"
     CDS             13023..13895
                     /locus_tag="U4A29_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO0680638.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSVATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14353..16551)
                     /locus_tag="U4A29_001510"
     CDS             complement(14353..16551)
                     /locus_tag="U4A29_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0680639.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16783..17775
                     /locus_tag="U4A29_001511"
     CDS             16783..17775
                     /locus_tag="U4A29_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0680640.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17835..19256
                     /locus_tag="U4A29_001512"
     CDS             17835..19256
                     /locus_tag="U4A29_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEO0680641.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19249..20748
                     /locus_tag="U4A29_001513"
     CDS             19249..20748
                     /locus_tag="U4A29_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEO0680642.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
BASE COUNT         6248 a         4257 c         3549 g         6708 t
ORIGIN      
        1 ctctttttta aaagtttttt tcgatttcgt ttttcgaaat ctttcagcaa ctcatttatc
       61 ataacttatc gtttgttatt tgtcaagaac ttttttgaag tttttttcga tagaatcaag
      121 cttcgcaagt ggttcttttt aacgacttag ttatcttatc acgtctaaaa acatgtgtca
      181 actctttttt ggaattttca taaaatgttt caatgaagcg cgacaacgtt gtttaatata
      241 tcattaaaaa aaataaaggt caagctaaaa tattatttaa gcttgacctt attataattt
      301 caacgattgt caaaagtatt gtttgcagct gtgttgccag tttctataga taaatcacag
      361 cttttgaaac ataagttttg tactgcttcg attcgcccga attcttttgc agttatttgg
      421 caatgagaaa agcagacatc ttctataggc ttttcaggaa atgctactaa atcaaaagct
      481 cgggaaggaa gtgagtaacc tggagaaagg catgcctgta cgtttttgat gtgtatgtct
      541 ttgactttcg tcatttgctt tgattcaggt atttcttcag ccactgcttg ccaactttct
      601 ggcatttgag caaggtcatt gctatttttt ctattgtatt cattatgcca gtccattatc
      661 caagaaaaag gaaattgtac atcaatcata ttcagatttt ctaccctgat attttcgatc
      721 actccccctc tttcttttga agatttcatt cgaataccac agtctgactg aaaaaaatcg
      781 atatcgtgaa tatatacgtc actaactcca gcactaactt cgctaccaat agttacaccg
      841 tatcctgaac ggattttaca tctagcaacc tcgattcttg atgattttcg attgactctt
      901 gcaccattgc catctctgcc agacttaata gcaatacaat catcaccaca ggccaaatca
      961 cattcataga cacgcacatt tgttgaagaa tcaatatcaa tcccatctgt acttggtcca
     1021 tcattgttgc ggatgattac tttttctact aatacatcgt tagaataagt gatttgcaag
     1081 ttccaaaaac ctgatttctg aaaaatcaaa tcacgaacaa caacatgttc tgcatgatac
     1141 agtaaacaag cgcgtggacg tttaatagca taatccgcaa tccaacgaag tcccttttga
     1201 tcgtattcag ctcttgtccc gcttttttga tcttttcccc aatacagctc ccaccaatgg
     1261 tctccttgac cgtcgattat tccttttcct tctataagaa tatctttcgc cgagagaaca
     1321 ttcaaaatcg ctgctggcca tttcatttct acgccggcta ctcgtgtatc gatttctgga
     1381 aatgcttcga tatctgttct ccccttgagg actgcacctt cttcaaagat aagatgactc
     1441 ttatctttta aaaaaagggc tccgactaaa taaatgcctt ttggaataaa tacaggtaag
     1501 tgttctttcg caccttgatc gattgctttt tgcagtatta ttgtatcatc ttgttgagtg
     1561 tcgtttgttt taaagttcaa agcattgata atcccttttt ttgtccgcaa tacgtttctc
     1621 cctatctttc attgattttt ttcaattctt caccaataaa tacagttttt gaacgattca
     1681 gtgggtaacc tactagcaaa atgattgcgg atacaaataa gcagaatgct ggtaacaagt
     1741 ttgccatcgt ataaatcctt tcttgtacta aaactgattg gatggctcca cctgttgtag
     1801 acgattggta accaatgagt gccagcataa atccgcctac cgctcctgca catgcttgtc
     1861 ctacttttct cgcaaaagaa tttactccat atactgttcc atcttctctt aagcctgtct
     1921 tatattgatg ataatcaatc acgtcagtaa tgaatgccca gaccatcaaa ttgaataaac
     1981 cagatcctaa agtagcgata aataaaagaa tcagatatac ccatgcattt gttatatgaa
     2041 ggaaatacat cagcaagtac atacatgaag aagcaagtaa cgcaaatacg cttgcttcct
     2101 ttttaccaaa taattttgtc agctttgtag caaatggtgc taaaactact actgttccgt
     2161 aagtaaataa aagagcaaca gacattgctt gtttattatg gaagtaatca ttgaataaat
     2221 aagtcatatt cgtcccagct aaaatctgat tgatcacaat gaaaatgtca acaatgacta
     2281 aaataatcag tgctcgatta gctaacaaac cttttaccaa ctgagaagca gatacttttt
     2341 cgtttttgac aactcttact ctttcagtag tcaatttaca agttaacata tatgcgacaa
     2401 atgctattgc agcacatccg attgaaatcc aaaagaaatg attccctgaa acgacttgtt
     2461 gtccactctt gttatgggta tacattaaaa tcggaatgaa gaatcctgta gttgatccgc
     2521 caatcgcaga accaatgctt cggaacgtgg acaacgaggt gcggtcatta ggatttgagc
     2581 taattgctgc tgccattgct ccatacggga tattgattgt tgagagaaaa atcccataaa
     2641 caatgtatga aacaaaaata taagcaatct tcaccgtcat tggaagatgt tggacgaatg
     2701 gcaaaaacaa aatgacagtc actaagcaaa atggatactt catttttctg atccaaggaa
     2761 taaatcggcc agtttctgtt aactctgaga catcacataa tcttccaacc atcacatcag
     2821 caaacgcatc caaaattctc gaaatcaaga ataacgtacc tacgattgcc cctgaaacgc
     2881 caagaacatt cgtataataa atcatcaaga aactattgac taaacctaaa ataaaacagt
     2941 tacctagatc accgaacatg tagcctatct tatcggaaat cccaaattcc tttttttctt
     3001 tttttattgt atttgctgta acagcatttt gtgaaagcat ttgaattcct cctacctctt
     3061 ctagtaaacg ttttcaaaat tataactctt tttttcttgt ttttatattc acaatgttgg
     3121 ctttttattg cattatcttg acagataaaa aacaaaaaat aatctaaaaa cattgatgca
     3181 taagattgat aacttttttt acataacaat atatacaaac aaaaaaattc actaactagt
     3241 gaaaataagt tagtgaacct catttattaa agaattaaat tacagatgat cttttccatc
     3301 atacttgaaa aactcaaaat cagcataact tcctcttttt ttatttagat catgcactgc
     3361 tatcccgata aaatttccag taaaccctcc tgagagtata cacatgtttt tttgcggacc
     3421 tactttcttc cattcttcag ttgatacgtc tttcaaatga aactgtgctg tctctttctt
     3481 tacagtgact ttcaaaaaag tcttcttttc tgtgagtgga atcgtccaat cttctatttg
     3541 tgctactccg ttttctgatt ctatcatacg taggcatttt cccttgtcct cattccaagt
     3601 aacataacaa taaatataat tggattcatt taagtacagt accagtccag ccatctgatt
     3661 aaaattatta ggcatgtatt caaatgctgt ctccgcatca aaagtaaaat ccgtttgccg
     3721 aatagctaac aaatgatgtt cgaaggtaga ttgaagagat tcaccggaaa tcagacgtaa
     3781 aaaacctggt ctacattttg tatcacacca atcagcattt ggcaaaatgc gtctggcatt
     3841 ccagttcttc tttagctctc cttcaaatgt atccataaac tgatgatctt tttcttgttg
     3901 tacctcttca tttgtctcaa tcattatttc ttccatcgga gttgtctggc tatctttaag
     3961 tcttaaccaa tcgtcggacg tccaatacac ttcttgaata gcggtttctc ttcccaaaat
     4021 agccgcaccg tttaatggtc tggtacaaag atatgtcatg taccatcttc ctgacggggt
     4081 ttgtactaag ctcccatgtc ctgtacattg caatgtagaa gcaggtttgt cactagctgt
     4141 cagcatagga aaaccaggat caagctcgta aggaccagta atattctttg ctcggcaaac
     4201 tgtaactgaa tgtcctcttc ctgtaccgcc ttctgccgtc atcaaataat aatacccgtt
     4261 acgaaaatac aaatgtggtg cctccgtttt cgctaattct gtacctgcaa atattttata
     4321 gatgggacca tcaagcttct tcttctctgc atcatattct tgtaatacga taccgctgga
     4381 tttattcggt gtgtccagac gataatccca tagttcattg acaagccatt tttttcccgt
     4441 ttcaagatca tggaacaaag aaggatcaaa gccgttacta tttaaataaa ttggttcgga
     4501 ccaaggacct gtgatctcag atgcagtgat caaataattg tggacatctt taaacggacg
     4561 gcctgcactt ttgacatctg tgtaaacaag ataaaacaat ccatcagcat aactgatctg
     4621 tggcgcccat atacttccat ttgtaggatt tccttgcaaa gatacttgag tggtcaaaat
     4681 atccgttgta tgtgaccagt tgactaaatt tttcgactca tatactcgga ttcctggaag
     4741 ccattcaaaa gaggagacaa taatgtaata ccgctctttc actcttaata tattaggatc
     4801 tggattgaat cctcttaaaa taggattttt cactttgata gtctgcatga ttcaggctcc
     4861 ttttctcatt cttactcgat ataaactgaa tgccccagct gcgaaataaa aaaatgcaaa
     4921 aggaaacgca ataaactgag tgccaatcaa caatatgaat cctgttataa aatacaaacc
     4981 agtggcagtt gttaagtatt ttcctttagc agtcaaaaac aatgctactg ctaaaaaagc
     5041 aagtgaaata aacaacacaa cggcaaacag accagtcatg tcctgtaata atccaaatgt
     5101 attttctaaa gacaaagttg aatgatctac agcaagcgac tgataacttt ttttaaactc
     5161 tgacgcagac atattactta aagtaactgc taacccgcca aagaaaatca aagatagact
     5221 gccaccgacc attcctagca atagttccca ttttcttttc atttctgaac cccctgcttt
     5281 tctaaatgta tccattcagg tcttttgatc gatgtttcat aattgttatt acgttcagag
     5341 aatttgacag tctgttcatt tttcttgtca tcccaattat cccaaccagt tgggtgaaca
     5401 tgcccaccta tcttacacgc ttcaaataag acatttgcat acggacgcca cggtcttcct
     5461 aaatagacgt ttctagtatc aggttctgca gtcaaggtac agctttcaaa acgataccct
     5521 gttttttgat ttttcggcgt agatgctgct gtgatgtatc caggattttt actggctaag
     5581 ctatggatat gacactgatg aaaaacggca tctgccccac caaatatgaa atccaccgtt
     5641 ccttcaatcc agcaatattc ataggtctga cgacaatact caggattggc agaagccggt
     5701 gtacaaaaag gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca taaggtatcc
     5761 tgataaccag atagtcgaca gttttttaaa gatacgcgat ctcctgaatt gaataaagca
     5821 acagcctgtc ctaccttttc tcctggacct gctgtatttt cgatgtgaag attttctaat
     5881 acgacattcg atccttctaa gaaaaaagta gcacttcgga aagtccctaa ctggctaccg
     5941 tcaagatact cctgttttgc aaaacgatta ccggagataa ctactgttcc tgctcccaca
     6001 agtttaaaat tggataatct gctttctata tattcatcat atcttccttc ttttactatc
     6061 aagacctttg gatggctatc cggcaggcct gccaaataat cgattccttc ttggatagtt
     6121 tcaaaatccg tcggggtatc tatagcattc tttccaatta tcagtttgtc tttcatcctc
     6181 ataaatgctc ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt tccatatgac
     6241 cgcctgttaa agaaaaagaa gaaaagtttt ctggactacc cattgattga tactttttgt
     6301 ccagcccttt tctcagcttc agtacgcctt cgtccaagca catcgtatcg ttttttccaa
     6361 ctaagctaag acgagggcgc ggagcgattt tttcttggat tgctaacgtt gtataagctg
     6421 tcaatagatt tggcacatag taataaaacc catgatgatc taggcggcga tgtagcaaaa
     6481 gtatttcgat gtccacctgt gccgctagat caatacaaac tttgacacga gaatccaatg
     6541 ctgtcaccca ccagcttaat aagcctccca tagaaagacc aatcgttgcg agccgttgag
     6601 tatctgtgtc ttcccttgtc tccaaataat cgatcaagct gatcacatca taaatacgca
     6661 ttccccataa cgtttttcct gttaataaaa attgtttgaa caactcgctt tcagaaatac
     6721 ctccgcgttc ttcaaatccc caagcatcaa tcgaccaaac agcatatccc attcctgtca
     6781 gtacttctgc aaaagatgga gaagctaagt aaggagcacc atggatcaat tcacttttcc
     6841 cgactgaaaa atctccgccg tgagaatgca ggtaaaggac cggcggaaac ggtccttctt
     6901 tttctggata agcatagagg gctggtactg tttccaattc atttaagtac aattgatagt
     6961 attcaagcat aaaaccgttt tgtttttctc ggtataatcg ttttccacta ggggtatgtc
     7021 tgctagatgg agcacctaaa aaatcattca actgcataag caaagttttt acgaacaacc
     7081 agttcatcca tattttctga ttgatttgat tggaatttct caatactgat ttctttagta
     7141 ttttctaata gaaatgctgg tccctcgtga ttttcaatcg acatctggtt aaaggaaacc
     7201 ttacccgcaa agccaatata aaatccttgt ctgctcattt cctctattcc tgtcatcata
     7261 gcaggaacac cgggaactgc atcttgggcc atcgagacat cgatattgtc aaatgtgatt
     7321 tcttgtatga attgttccgc taatccataa ataaaacctg cagaagcatg gacattcctt
     7381 gccgtgatat tcgaaaaatg gatccttctg aatgctggtg tccgttcatc tatcggataa
     7441 gccttttttt cccatacata cggttctttc cctcgcggac cacagaaata ataaagattc
     7501 agtataaatg gacacatcac attatccatg actatattgg atactcgaat atcttcaacg
     7561 atcccgcctc gacctcgtct agatttcaat cgtattcctc gatcagtttc ttggaagata
     7621 caatttgaaa tggtaatatt acgaatacta ccactcattt cacttcctaa tacgacaccg
     7681 ccatgtccat ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc gatcctttcg
     7741 tacgtatctt cagtccctgc cttgattgca atacagtcat ctcccacgtc aatatggcaa
     7801 ttactaatac gcacattttt gcatgattct ggatcaattc cgtctgtatt tggtgaatcc
     7861 gctgggttca agatcgtcaa gttatcaaac gttgcattac tgcaaagaat cgggttgatc
     7921 gtccaacttg gtgactggat caatttaata tctttcacag taattcgatg acagttatgg
     7981 aaactcatca atttaggacg aggataagcg agattatctg gttcatttct aaaagtatgc
     8041 caccattttt ttccatttcc atctaatgta ccgaaaccag tgacagagat gttctctaca
     8101 ttttgagcat agatacagga agcataaact tttcgatgga ctccttccca acgtgaatga
     8161 acaacaggat aatcttttgg atcgtcagag aatttcagaa ccgctcctgc gcttaaatga
     8221 agttcgacat tgctttttaa aaataaagct cctgttagaa attcacctgc tggaacaact
     8281 acggtcccgc ctccgtcact tgctgcagca tcaatggctt gttgtattgc ttcagtattc
     8341 aattcatcaa ttgatgcgcc aaatgtcaaa atatcataca tatttataat gcctccactt
     8401 caatcaatgc ttgcaagaat gcaccaacgc ctttttgatc attacaaata attggttcac
     8461 tgatgtagta agcatatgaa ccatctcgct gatctgctcc gcctaaccct gctacttgac
     8521 agtttttatt caagttcagc cagccttctt ttgtttccag aacaaattca tcaagtaatc
     8581 cttgatgtga tttcttcact tgtggaagcc attcttcttt gttcagcagt cccaaacgta
     8641 ttcctttagc cattgcgtaa acaaacatac tactgccgga agcttccaga taatttcctt
     8701 tccgttttgt ttctgttgtt acttgatacc atgtcccgct gttttcttct tgatatggaa
     8761 gtaatgcagc cagtgtcttt tctagaattt ctttcagcaa agaacaatct gtttcttcat
     8821 gaagtaattc ataggtatct gctgcagcca tcaaatacca gccaatcgat cggctccaaa
     8881 aatgatggga tagacctgtt tttggatcag cccatggttg gaccttcttt tcatcccatg
     8941 catgaatcag caaatttgtt tttggatcga atagatgttt ataactgatt tcaaactgtc
     9001 tcaaaacatc ttggtaatct ttttcttcac taaaattctg gataaattca gcataaaaag
     9061 gagaacccat atataagcca tccaaccaaa tttgataagg ataaatttct ttatgccaaa
     9121 aagctccttc ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt tctgctgctt
     9181 ttttatattt ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt cctgtattta
     9241 catggtcgat attgtattct tcaacgctgt agccacggat cgttccatct tcttgcacga
     9301 aatagtcgat attgtctttt atgaaatcaa aataaatttt ttcttgggtt cgttcccaaa
     9361 gtaattggaa ccctttaagt acgacgccat agtcataaga ccattttcct ttataccact
     9421 tttcttcata taagcgaggt gttctttcca tgatactttt tgctaatttc tttgaccagt
     9481 tttccattat ttgctctcct ttaaaaatta aaataggatt tgggacaaat accaagaagt
     9541 atttgtccca atcgtctata ttactgtgcc gctgctttgt atgcttcctc gtactcttgt
     9601 gtgattcgat cgcctccttg tgctttccag tcttctacag cttttttgaa tccatcttcg
     9661 tcaagctgtc ccatgatgta ttgcaccgta gcatcaatga cgattttttc aatttctgat
     9721 cctacttcat tgttcgtcgg tgaatccaat ggcacagaag gatccattac agcaaattct
     9781 tcattttctt tgatcaattg attagagaat tctttttcag ggtctgtatc tttgaaagag
     9841 aacgtcactt cactaggacg agagcttgat aacggttgca cctcttgttg ccaaagatcc
     9901 atatcgataa attcgataga accatcatca ttcaattcat aatgcgttcc ttcgataccg
     9961 ccagtcatca atttatacac atcttcttct aaaagatcat caacaaattg aagtaatttc
    10021 ttcaattgtg cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc gccaatacca
    10081 tttccttctg accaaacatg ataaccgccg ccttcaccat ctgagatctt gtttacaggc
    10141 actaattcca tatcatcttg aataccttga cccatcgtct tcagattctt gatatccacc
    10201 attccagtgt agattccagt tcttccttgt gcaaattgtt cttgctgatc agtcttttgg
    10261 gtaactgcaa agtcttgtgc caaatagcca ttctcaaata gatctcttga aaaattcaaa
    10321 gctttcatat actcttctgt atcaaattct ggagtgaatt tgccattatc gtctacagcc
    10381 cagccatttg gtgctccaaa ataagaagtg aaaagtttaa agctggtata acgaatatca
    10441 ctaggcgcac gatcgccaaa accaactgtg tcgtccacac catttccatc aggatcgttt
    10501 tctgtaaatt gtttagctac ttcataaagc tcatccatgg tagttggaac atctagtcct
    10561 agattatcca accagtcttt tcgaatgact aagccagcac gagcgtagtc tttttgtatc
    10621 ggtacgccgt acaaggcacc gtcgatactc gcagcttcga ttctatcttc agaaatttga
    10681 cttaaatttt tatagtcttt taaatattct gatacatccc agaatacgcc tgattttaac
    10741 gcattgcgga cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat atctcctagt
    10801 tcatcagaag ccaatgcagt tgttaatctt tcttcttttg aagcatcagg aatccagttg
    10861 aatgtcagtt ccgtatcggt tttttcttcg atcaagttca caatcgtgtc tgtaggcggt
    10921 gaagcatgat gcaaaatatt taaccaagtg attttagctt ttccatcttc tgtctctgaa
    10981 gaactgttac ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc aaaactaaga
    11041 cctaatttta cgagcgttgc ttttttcata aattttctcc ttcactgttt taataatatt
    11101 tttgttttct tctgtttttt ctgtatatga gttcttccat tccaaatggc aaaatataga
    11161 atttgctgtc cacgtgactg cggaaaagaa gagactgcca ccgataaagg ttaaaacacc
    11221 tggcattgtt tggatgatca tcagataaat ccctaccata cttaatatca acactaatga
    11281 gtaatgaaga tgtgagaatc cgaataaaaa tgccattttc attttgaaaa aatatccttt
    11341 ccaatcataa tgagccatga ttgggaaaat atagagtgct gagagcacat atatgaacgc
    11401 agcaatatac aaagcgatcc taagatacca attgtgtatg aaagaaagat ccacaagtaa
    11461 aacaatcccg atggcgccat atatccagct catcagcaag gtttcacgat agttttcttt
    11521 aaaatatgtg aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca gccattttct
    11581 aatcactgca accatcgcat acgatgcagg accgaaagta aaaactccca aacctgcgat
    11641 gactaaaccg ccccacagaa aattcaaata aaccaactgt aaaatacgat ttagccttcc
    11701 gtttatttta tctaatgact gcagcatatc tttccctctc tttctatctt agtaaacgcc
    11761 gtcttctcct agctttttgg caatcttatt agctaaaatt accataaaca atcctacgac
    11821 ccctttaaac aatcccattg ctgtactgaa acttaactgt ccattcttca atccagctgt
    11881 ataaatatag gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca ataacaagta
    11941 catatgctcg aaccctaagt ctaatgtctg accgattttt agaatcagca aggtaatgat
    12001 tactggacga attgccggca atgtaacatg ccatgttttg cgtaagcgtc ctgctccatc
    12061 catttctgca gcttcataca actgtgtatc gactgcagtt atcgctgaaa ggtagatgat
    12121 cgtagaccag cccaactctt tccagatcac ttgtccgata tatactgtgc gtgtccattc
    12181 aggggatgtt agaaaactga ttttttcaaa tcccattgtt gcaaggaatt cattcaatac
    12241 ccctccgtca acattcaaaa atacataagt aatcgaaaca atgattaccc aagacatgaa
    12301 gtgagggatg taaatgatcg tttgaatccc gtttttcaat cgtttgttcg ttacttcatt
    12361 taatagtaaa gacaaaatga ttggcatcgg aaaaaacacg aagatattta atccgaacaa
    12421 gatcaacgta tttcttaata atgtaaagaa tgtcggttct gtaaacaaac gaatgaaatg
    12481 tttgaaaccg acccaaggac ttcccattat tcctaaaaat ggttgataat cttgaaaagc
    12541 aataacgagt ccccccatag gaagatacct aaaaatcaag aaataaagga ctcccggcag
    12601 aatcatcaca tataaaaatt tattcgtttt gattcctgca attatttttt ttcgtcttct
    12661 gagcttttct tcttttttcg atagcgtcag ttgaaatggt tcttgtttct ttacactttc
    12721 cataagcccc tccttagttt tgtgaatgtt tgctgcttat gacttcatga tagataacta
    12781 tctgaattac gtatataatc atttttaccg taagtaaaaa cgcttacata gcaatgattt
    12841 ttgtgctata caaaacgata aagtgtattt tttcacaaat aaactacgca tagcaaacct
    12901 tctatttttt tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg
    12961 atgataaatt gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct
    13021 atatgaaaaa gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc
    13081 taattttgct agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg
    13141 cttctacaca agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag
    13201 acgcgtatcg ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg
    13261 gtgttacagg tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc
    13321 tttccagaaa gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt
    13381 tgttcagcgg ggggatgatt ccaacgttct tagttgttcg ttctctaggg ctggtcaatt
    13441 cttactgggc catgatcctg cctgtggctg ttaatgcaat gaatatgatc attatgagaa
    13501 actttttcca agccttgccg gatagtttgg aagaatcagc aaaaatggat ggttgcacag
    13561 attttggtgt atttttcaaa attatgctgc cattagcgtt accgtctgtt gctactatct
    13621 cgctttttta tgctgttact tactggaaca cttatatgac tgctattttg tatatcaacg
    13681 attcatctaa atggccgata cagattcttt tacgccaaat cgtcattgta tcaagtggga
    13741 tgcaagcaga aagtagtgcc gttgatgtga ttccaccagc acaaacagtt aaaatggctg
    13801 ttatcgttat tgcaacagtg cctatgctga ttgtttatcc ttttgttcag aaatattttg
    13861 tcaaaggagc actcgtaggt tctgtcaaag ggtgattatg tactgaacgc aaaatggtag
    13921 ttgtgtcaga agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat tgcttcttaa
    13981 atttttgtga aatttcggct tatttttcga agaatctact cctggaacac cgtgtatcag
    14041 aggttgtgaa agagacgttc agccttgagc gatgataaaa actctcacaa acattgcgac
    14101 gagtaaccca caggagcaat aagaagcgat tttgaaaaat agcttcccat atttcacaaa
    14161 attatgcctt attgtcgata gcctgtctct tgaacgccat ttattcgtaa aaaagaactg
    14221 tgacaagact tttgtcacag ttctttttgc gtgcttagcc ttgaaattta actatccata
    14281 aatacaaata ctagggttat tctaacttct tttattgctt gaatcagtcc acaagaaaaa
    14341 cagcctcttg attcacaaat atttttttcg atattctccc ggcgtaatcg attccttctt
    14401 tttaaagaaa cgaataaaat tttgcggatt tcgatattgc aatctttctg caatctcttt
    14461 tacggagagg ttagtctctt tcaaccattt ttttgctacc tctaaacgat gattttgaac
    14521 aaaatcacca aatttttctc ctgtttcctt tttaaataca ttacttaaat agttagggtt
    14581 ataatgcaac ctatcagcga tcacatctaa agacagatca cgatcatatt ctgtgtagac
    14641 gatatgcata atattctccg acaaattttt aaattcatga tcagtgattg cttgagagca
    14701 ttctgtgatt ggcagaacta attgttctaa aacaagtttt tctagttctt tgggatgata
    14761 attgttcagt gcttttacat aaatttgttt catgttatca aagttttttg aatctactcc
    14821 caataactgt cctaattgaa cgagttcatt gatcaagcgg accgaaacta cttcccgact
    14881 caaaggattt ttattttgtg caaacaatgc atcaatcaat tcatgtacta aattcttcac
    14941 ttcctgattt ccagaacgaa tggcttcaaa caatcgattt tgttcttcta tcggatatct
    15001 aataagtgtt ttttcatgag cagcaggaac gatttcctga taaaaaataa ttgaacttgg
    15061 accagtattt acacgataat acagggcttc ttttgcccga tcaaccgatt gcttgctttc
    15121 attcagcgtt tcgaaccggt tactgatgcc tatactgata gttaaattca agtattcttt
    15181 tacacgtttt tgaatgagtc ggcaatactc catgactttt ttgcttgctt ctgcatcatc
    15241 tttatccatt cgataaatag tggcttgcat ttcttcattc aaaacgatcg gcagcattcg
    15301 attttccttt ggcacaattt ccgtgatcat gttgttgatc gctaaaagta gcaaatcacg
    15361 ctccccaccg tgactatctt ctaacagatc gatttgaacc aaagctgtat aatagcagtc
    15421 attttgagaa ctataaccaa attgctgcaa ccgctgatct agttctcgcc tttctactcg
    15481 atttctaaat aaactaagta caaatagcgt ttccagctgt ggcttttggg taactaagtg
    15541 tgcacttaac gcttcttttt ctcctatgat ccgactgact gattgagcga ccaaatctag
    15601 ttctttccca ctgaatcctt ttgatttaag attcagacgt tcctgtattt gagaaatcgg
    15661 acgggcaaat ctgtccgaaa ataagtagga aagaagacca atgagtccta ccagtgttaa
    15721 agacactgca actagcccga ttctcagatt gcggatcatt gcgccaattg cactagagtc
    15781 aacttctaat ctgtaaatcc aattattata atctgatttc agtactatat acgttttatc
    15841 tttttctctt atcactttca gctgattgta atctagatcc acgtccgtta atacagttgg
    15901 ttgctcttta ttttttactg tctgagtagt aaaaaggttg ccaacttcgt tttcaataga
    15961 aagtatgtct tctccacttt caccaacaac ttgttctatt gtatgtttct ctatcgtagc
    16021 aattcccaat gcatattttt ctttttcaaa cattggcaaa gaaatcacca tttcgattcc
    16081 tttgtttttc ttttgccaaa ataaattatt tttgttattt atgtacttct gttgatactt
    16141 ttcaatttcc tcttcggtca gttgactcaa cgatccattg attactcccc atttaccagc
    16201 caaactgact aaatcataat tattatcatc cataacaatc gtttctataa aatttagttc
    16261 tttcctgatt tcagaataag tggagtaatc tgtatatgtc agcggatgct tgaatatgtc
    16321 cgagaaattt ttggtcgaac catacgtaga aaacgaatat tcaatcgttc taattttcca
    16381 ttcaacgtta gttttaactt gcctgagcaa attctttttg tctgttaaat aactcgattc
    16441 gatcgattca taggtattct ggtaaatgaa taaactaaat cctatgacaa gtacggctcc
    16501 taataaaaac atcggtaaaa atattttata aaatactgta cttttcttca ttttcttcct
    16561 ctcctccttt taaaataaaa ggctttcatt tttctgtaaa attgatactc atctaattaa
    16621 atgagcattc cccgctttca agaatagcat aattaaaaat aaaaatccaa gtaattttta
    16681 acgtgtgcaa tcttttcata tcaaagatct attgacttat cgtagttatc gtaatatgat
    16741 gaatgcgtta acaaaaagtg aaaaagtaag gaaaatgatg gaatggtaac gatcaaagat
    16801 gtcgctaaag cagctggcgt ttctcatacg acagtttctc gtgctttgaa taacagcagt
    16861 ttaataaaaa atgaaacaaa acaaaaaata cgacaactag ctaaggaaat gaattattcg
    16921 ccaaactata gtgcaaaagg tttagtgaat caaaaaaaat atctaatcgg actatttttc
    16981 tcaagtttgc ataaaggaac ttcatcaaat ttcctagcag attccattgc aggcatccat
    17041 tcagtattag acacaaatta cagtttatct gttgaaagta tcgatgttgt tgatttaaca
    17101 gaaatcaacc tgcaacgcta cgatggactg atcgttatga gtcaaagtga tgatgatcag
    17161 cctttcatcg actatctcaa agaaaaagaa tttcctttcg ttgttgtcaa tcgtcacatc
    17221 aatgatccag cggttatcaa cgtcactgcc gatgatgcgg ccggtgttat gcaggcagta
    17281 gattacggta ttggatgcgg acatacgaag atcgcttata ttggcggaaa gaaaaatttt
    17341 cgttcgacaa aagaacggga aaaaggggta ttgaaaagtc taaaaaaagc gagcatccct
    17401 gtcaatccag cctatttttt aacaggagat tacagcttag aaagcggcct ccaaaacatg
    17461 gaatacctat tatcactttc tgacctgcct actctggttt tttgcggcaa cgacgacata
    17521 gcgattggtg cattgagagc tgtacattta gctggcctcc gtgttcccca gaacatttct
    17581 ctgatcgggt tcgatgattc tccagtcgtc tcctatctca atccgccgct taccaccgta
    17641 cgtaagcctt tgaaagaaat gtgtcaagaa ggaacaaagc ttttactaga tttgatcgaa
    17701 ggcaggacga tcgaacaatc taaaattgag ctctccacct tgttgatcct tcgtgagtcg
    17761 atcaattact tgtaaactac aagtaatttt atataaaaat gttaacgtgt gcataacaca
    17821 gaaaggaagt ttatatgaaa aaactgacaa aagaattagt gaaaaaaaac gaagcacctg
    17881 taaaagttct tcagtttggt gaaggcaatt ttatgcgtgg atttattgat tggcagattc
    17941 agcagctgaa taaccaacaa ttgtttaaag gtaatgttgc aatcattcaa cctttagaac
    18001 aaggtcttgg aaatatgatg aaccagcaag ataatttata tacggtcatt ttacaaggac
    18061 tcagcaatgg cgaaatcgtt gatacttccg agattatcac cgtagtagaa caaatgatca
    18121 atccttatga tgaatgggat gcttatctat ctctagcaga aaatgacgac ttggcctata
    18181 tcgtctccaa tacaacagag gcaggaatcc aattcgtgga gactgataat ttaacagata
    18241 ctcccccaca gagcctcccg ggcaaactga cagccttttt ataccgacgt ttcaaattaa
    18301 acaaagctgg ctttaccatc attccttgcg aactgattga cagaaacggc gaacgtctaa
    18361 aagaaatcat tttacgctac gcagaaaaat gggatttaga acctgctttt gttgagtggt
    18421 tagaaaaaga aaatgttttc tgttgtagtt tagttgatcg catcgtccca ggttatcctc
    18481 gcgaagatgc aggtgttttc aatcaacggc ataactatga agatcaattg atggtcaaag
    18541 cagaaccatt catgctttgg gtgatcgaag ggcctaaaga attggagaat caactaccat
    18601 tcaaacaggc tggcttgaat gtcatcatca cagatgatat gactccttat cgtgaacgta
    18661 aggtgcattt gcttaatgga ccgcatacag cgatggtccc tcttgctttg ttggcggaat
    18721 tggaaactgt agaagaagta atgaaagatc ctttattctc cgcatatgtt gatcagttat
    18781 tcaatctaga attgattcct atgctttctt tacctaaaga tgaattagcc atttatgctg
    18841 accaaatcaa agaacgtttc ttgaatcctt ttgcccatca caaattagaa gcgatttcat
    18901 taaatagtgt ttcaaagttc agcacccgtc tgcttccagt gttcaagaag tatatcgaag
    18961 aacaaaatca ggtaccaccg ttaatcactg tttcactagc tgcgctactt cttatgtatc
    19021 gaggcgatca agtaaagcca catgatgatg aaaagacgat aagcgaattt actgatgctt
    19081 ggtctgataa tggaacagct attcctcgat tattgcaaaa cgctgcgctt tggggagaag
    19141 atttgtctca aataccgaat gtgacagata cagttcaaga aatagctgca aagatcgatc
    19201 aaaaaggtgt ccggtcagta ttacaagaaa tgaaaggaac gatggtccat gcttgaaacc
    19261 ctgcaaccaa caatgaaatt aaatcgacga gacagtgtgg tcgtcgcatt aacccctatt
    19321 ccaaaacaaa cacaactgac gattgatcag caaacgatca ccactcttga agatatcccg
    19381 caaggtcata agattgcatt gggtgatttg aaagctggag acaacgtgat caaatatggc
    19441 tatccaatcg gtcatgtgac gacagacgta actgccggtc aatggctgca cactcataat
    19501 gtaaaaacca atctttctgg tgaattggat tatcgatatg aaaaagatgt ccatccaagt
    19561 cattaccttt ttgagaaccg aacatttcaa ggttacttgc gaaaaaatgg aaaagtaggg
    19621 attcgtaatg atttgttttt agtacctacc gttgggtgtg tgaatggcat tgctgaactg
    19681 atcgtcaaac agttcaagga aaaacatcca gatctgggac agtttgacca tattacgatt
    19741 ttaaagcatc cttatggctg ttcgcagctt ggaaaagatc atcaaaatac tcgagaaatt
    19801 ttagcggacg cagtaaatca tcccaatgcc ggaggagttt tagtatttgg attaggttgt
    19861 gagaacaata cagttcctga attcaaaaaa attttaggtg aatatgatga agaacgagtg
    19921 aaatttctag tggctcaaga tgtttacgat gagatcgaac aaggtgtggc tctattagaa
    19981 gagcttttaa cagcggcgga aaatgaccag cgcatatcag tcccgctctc taagttgaat
    20041 gtcggtttga aatgcggcgg ttctgatggt ctatcgggaa taactgctaa tcctttactt
    20101 ggtgcatttt ctgactactt gattgctcaa ggaggcagca cgatcttaac agaagtgcca
    20161 gaaatgtttg gcgcagaaca agtattgatg gcacgagcgg aaaatgaaga agtttttgac
    20221 tcaatcgttg acttgatcaa tgacttcaaa cagtatttct tgtcttacgg ggaaccagtt
    20281 tacgaaaacc catctcctgg taataaagca ggtgggatca ctacattaga agataagtct
    20341 ttaggctgca cgcaaaaatc aggaagctct cctgtcgttg atgtgctaca atatggcgaa
    20401 aaaatcagaa aaccagggct tagtttattg caagctcccg ggaatgatct tgtagctgcc
    20461 tctgctcttg cttcatccga ttgccagttg gtcttgttta ccacaggaag agggactccg
    20521 tttggctcat atgtcccaac ggtcaaggta tctacgaata caactctttt tgaacgcaaa
    20581 ggtcattgga tggattttaa tgcaggcgtc ttattagaac aaccaatgga agtaatctta
    20641 gaatcgtttg ttcaaaaaat catcgctgta gctagtggag aagaaacgaa aaatgaacaa
    20701 aatgacgttc gagaaattgc catttttaaa aacggagtaa cactttgatt ttcataaaac
    20761 ag
//
LOCUS       DASOUD010000046        20287 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.046, whole genome shotgun sequence.
ACCESSION   DASOUD010000046 DASOUD010000000
VERSION     DASOUD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.046"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1385)
                     /gene="cls"
                     /locus_tag="U4A29_001514"
     CDS             complement(<1..1385)
                     /gene="cls"
                     /locus_tag="U4A29_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO0680643.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETV"
     gene            1726..4392
                     /locus_tag="U4A29_001515"
     CDS             1726..4392
                     /locus_tag="U4A29_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEO0680644.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4757..5509
                     /locus_tag="U4A29_001516"
     CDS             4757..5509
                     /locus_tag="U4A29_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO0680645.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5531..6685)
                     /locus_tag="U4A29_001517"
     CDS             complement(5531..6685)
                     /locus_tag="U4A29_001517"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO0680646.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            6870..9311
                     /locus_tag="U4A29_001518"
     CDS             6870..9311
                     /locus_tag="U4A29_001518"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO0680647.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9641..10345)
                     /locus_tag="U4A29_001519"
     CDS             complement(9641..10345)
                     /locus_tag="U4A29_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680648.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10532..10777)
                     /locus_tag="U4A29_001520"
     CDS             complement(10532..10777)
                     /locus_tag="U4A29_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680649.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10794..11645)
                     /locus_tag="U4A29_001521"
     CDS             complement(10794..11645)
                     /locus_tag="U4A29_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO0680650.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11811..12815
                     /locus_tag="U4A29_001522"
     CDS             11811..12815
                     /locus_tag="U4A29_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO0680651.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13056..13412)
                     /locus_tag="U4A29_001523"
     CDS             complement(13056..13412)
                     /locus_tag="U4A29_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEO0680652.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            13780..14967
                     /gene="tuf"
                     /locus_tag="U4A29_001524"
     CDS             13780..14967
                     /gene="tuf"
                     /locus_tag="U4A29_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO0680653.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15155..17746)
                     /gene="mprF"
                     /locus_tag="U4A29_001525"
     CDS             complement(15155..17746)
                     /gene="mprF"
                     /locus_tag="U4A29_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO0680654.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(17772..18533)
                     /locus_tag="U4A29_001526"
     CDS             complement(17772..18533)
                     /locus_tag="U4A29_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO0680655.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            18700..19332
                     /locus_tag="U4A29_001527"
     CDS             18700..19332
                     /locus_tag="U4A29_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO0680656.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            19391..20251
                     /locus_tag="U4A29_001528"
     CDS             19391..20251
                     /locus_tag="U4A29_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEO0680657.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
BASE COUNT         6528 a         3636 c         4156 g         5967 t
ORIGIN      
        1 actgtttcag ttgtcatgga tgaacaatgt gtctcatctt ctttgaactg agtagcaagt
       61 tcttctaaaa actcaggatc gtaaatgaca gcacttgttt caaaattcaa tcggtagcta
      121 cgaatatctt gatttgctga acctaccatg cagatttcat catccattat caatgttttt
      181 gcatggagga agccgttctc atagacaagg atttgtacat tttccttgat cagctgccga
      241 gcataatatt gtgttgctcg ataaataaaa ggatgatccg gtttgttggg aatcatgatt
      301 ttcacatcta caccagaggc agttgcgatt tttaaagcag caatgacact ttcatcagga
      361 acaaggtaag gtgtctgtat ccaaacacgt tttttagcag aagtaatcaa tttgataaat
      421 gaaagcttga tttgttccct ttcgttattc ggtccgcttg aaacaagctg gatggatgta
      481 tcagcaagct tagattcatc tcgatctgct tttttaaaga aataattcaa atgatagccg
      541 accttttttt cttcaggtac cgagacgttc cagtctgtta agaagcgaag ctggagcaaa
      601 gaagaagcgg caccgaaaat ccgtatatgc gtatcgcgcc aatagccgaa tttttttgtt
      661 gtatttacat attgattggc aatattgaaa ccaccggtat aactaatctt tccatcgatc
      721 acaacgattt ttcggtgatc atggtaattc aatcggaaac gaagtaatgc cctttgtgag
      781 gtaacaaatg tatggacatg tccaccattt ttgattagac gattgaaatc ttttgctttt
      841 gtgccatgag agccaaatgc atcatataat attcgaactt ccacgccttc tgcggctttt
      901 tcttctaaaa catgtaagac ttgctggccg atattatctg tcacaaatgc gtaatattcg
      961 atatggatcg aatgtttggc tttttttaga tcttgaagca gtgaatccaa tttctcttgt
     1021 ccgtctgtga gaagagtgac agaattcatt cttgtcagcg gcatacgatt taatgaagag
     1081 aagaagtcaa caaattgctg tttgtcttta tcgcccatgt caggatcgta atgttcaatg
     1141 gtatctcctc taaaagactg aaagttttct aattctttca agtcactttg tcggagatag
     1201 aattttttct tatccgttaa tccacgtccg aaaaataaat ataatacaaa gcccacccca
     1261 ggaagggcaa acaatactaa gagccatgcc caaatggctg ctacatctcg gggtttgatc
     1321 aaaatagcca ccaaagcaat aagtgcattt aatagataaa gggcggttat aatactagat
     1381 accacaaaaa tgttcctcct tacatacaat aacattagta tagcatgata gaagaaagaa
     1441 gcctaccaag gtgaatatac ttttcactcc tttcatgaaa atgtaggtaa atcgggctaa
     1501 agtagttgct ttttgtaggt taataaggta ggatattagt acagttgaat atctccttag
     1561 gggagtaacc ggcaacgttt gttgcgataa tatcaacagt aagttaacca aatggttttc
     1621 tggtattgta tgaaatggtg agacctaaga caaatgcgta tttgtcttag gtctcttttt
     1681 tttcagacag gactagggcc aagatcaaaa aatgggggat gaaatttgga aaacaaggaa
     1741 caagcacaac aagcggtcta taaagaaacg atcgactcgt tgatgcagca aacgcagagc
     1801 tcaaccgatg gattgagcac cacgcaagcg aaggaaaaat tagaaagaga tggcttgaac
     1861 cagctaaaag aaaagccgaa gaagccaact tggaaattgt tcttagaaac tttcaaagat
     1921 gcaatggtta tcgtattgct gattgtcgca ctgattcaga tggtcatggg tgactacatc
     1981 gaattcttag ttatttttgc agtattgatg ttgaattcta taatcagtgt tgtccaaaca
     2041 aaaaaagcag aaggttcttt agaagcgttg aaaaaccttt cagcgcccga tgctcgagtg
     2101 attcgtgatg gtaaggaaaa aacgattccg gcaaacgaac ttgtggtagg agatatcgtg
     2161 ttgttagacg caggcgatta tgtacctgct gatggacgtt tactagatgc gggatcatta
     2221 aaagtagatg aaggtatgct gacaggagag tcgacttctg cagaaaaaga agtaacggat
     2281 atcacaagta cggtaccaat cggagatcgt acaaatatgg tattcagtgg aacgatcgta
     2341 acttacggac gcggtaaatt cttagtgact tcaactggga ataatacgga gatcggacaa
     2401 gttgctggcc ttttggaatc aacaactgag caagtcactc cgttacaacg aggtctggat
     2461 aaattcagta aaaaactaag tttggctatt ttggcattgt ctttattgat tttaggtatc
     2521 caactgtttc gtatttatct tggagaaggt acaggagata tgactgcaga tatcgtcagc
     2581 gcagtgatgt ttgccgtagc cgtagccgta gctgctattc cagaagcatt acagtctatc
     2641 gtaacaatcg tgttatcttt gggaacgaat aaaatggcaa aacgtcatgc cattattcgg
     2701 aagcttccag cagttgaaac acttggttcg acaagtatca tctgtacaga taagactggt
     2761 acattgacac aaaacaaaat gacagttgtt gactactttt taggtaatgg cgatactggg
     2821 gatttcacaa atgacccttc aaaatggaca gctgacgaac gtcgcttgat cgaaatcagt
     2881 gttttagcga atgatgcatc gatcagtgaa gacggtacta aattgggcga ccctacggaa
     2941 gtagcgttta tcgactttag tgaaaaatta aaccagcctt atcaggaaat ccgaaacaac
     3001 tacccaagac aagcagaact tccttttgac tcagatcgaa aattaatgtc tactggacac
     3061 acaattgata acaaaaatta cttgtttgtt aaaggcggac cagatatcgt cttccaacgc
     3121 agtaaaaaag tattgctaaa tggtgaagtc gttccgatga cggatgaatt gatgaaaaaa
     3181 ttccaagagc aaaatgaagc tttttcaaca cgcgcattac gtgtactagc ttttgcttac
     3241 cgtccacttg ataattttga tttatcattt gaagatgaag acgatttgat ccttgttggt
     3301 ttgcttgcta tgatcgatcc tccacgtgct gaagtgactc aggcggtaac agaagcaaga
     3361 agtgcaggaa tcaaaacaat catgattact ggtgaccaca aaacaacagc tgttgctatc
     3421 gcaaaacaaa tcgggatttt tgaagaagga aacctggctt tgaccgggac ggaactagat
     3481 gaattgacag aagatcaact aaacgaagtt ttagaaaaag tcactgttta tgcacgtgtt
     3541 tcacctgaga ataaaattcg tatcgttcgt gcttggcaaa ataaaggtca tatctcagct
     3601 atgacaggtg atggtgtaaa tgacgcacca gcattgaaac aagcagacat cggaattgct
     3661 atgggaacgg gtacagatgt ggcaaaagat gcagcagcga tggtattgac ggatgataac
     3721 tttgcttcta ttgtcagcgc agtagaagta ggacgtaatg tgtttgataa tatcaagaaa
     3781 gctatttctt atctatttgc aggaaattta ggtgcgatca ttgcgatcat ctttgcctta
     3841 gttgctgact ggtccaatcc atttacagca ttacaactgt tgttcatcaa tttagtcaat
     3901 gattcactgc cagcaattgc attaggtaca gaaaaagctg aacctgctac gatgaaccgc
     3961 ccgccaagag atccaaattc aggaatcttt acggacaaaa cattgatttc tgtaacgtat
     4021 cgtggtgtgc tgattgggat tgctgttatc tttgctcaat ggcttggcat gcagcattca
     4081 cctgaattcg gtgtagccat gtcattttca acattgattt ggtgtcggac attgcaaaca
     4141 ttcccagcac gttcaaacac acaaacagct atccaagcag ggttcttctc taacagaagt
     4201 gtcttactag ctgtagttgt atgtagcgcg ctttactgct taacaatgat cccaggactg
     4261 aaagaagtct tctctatccc atcagcattt ggattgacag aagtctttac atgtatggga
     4321 atcgcacttg tgacaattgt tttgatggaa atcactaaat tgatcattgt acgagtacaa
     4381 ggaaataaat aaaacgaatc aacgaggttg tgagcctgcc gtttaattct gagtcacaag
     4441 gtgcaatttc aacaaacagc ttctcatgtt tgttgaatat tgtgacttgt gaccgaagag
     4501 ttggcaggag aacaccgttt aaggaagaag ttgtaaaatc agcattttga gctaatactg
     4561 agggtcatct gattggacac tgtttattcg gaaagaggga ctttgacacg actttggtca
     4621 cagtcccttt tttttattta tgatagacgg aaagccaatc attttattat tcatcaaaag
     4681 agatagaata gataaaagag gagaatgatt tattccgaaa agtgaaaaca tataacaaaa
     4741 aattagagga aatcaaatga atgaaaaaga atgggatgaa tttgctgaag aatattatga
     4801 aatccagcaa gaatcgcaag taactattgc tgaagatgta aaaaattatc tgaaaacgga
     4861 gaaagtcctg cctgctgaaa cagcagcaga cgttgctgga ggatctggca gatttctttc
     4921 cttagcaaaa gaaattaatc gctatgattt gatcgatttt tcttcaggaa tgctagaaaa
     4981 tgcaaaaaaa gaagcagaaa aaaatgagtt gccaaatgtt tcctttatca aacagtcgtt
     5041 agaagaattt atccaatcaa atgagacata tgaaatggtt tttacagcag caaatccagc
     5101 attggtacat cttattcaat tagaaaaatt gatgaaaaag tcaagcaaat ggtgcgtgat
     5161 aatcagagta gtggaatcaa tcgatgatgt tttttatcct atcgaaaagc aactaggaat
     5221 gatagaagaa gatcctaata catcgccagt tgtaatggat caatttgaaa catacttaat
     5281 tgatgaagga ttcaaagtga agaaaaaaga ttttacttat tttacacagg aatcaattac
     5341 tgaagaattg atgaagctat actatgacgc gtacgcaaaa gatcctgttg tgaccaggta
     5401 cattcgcaaa ctattcagtg aaaaacgaga aatccttagt acgactaagc tgacctatag
     5461 actgctcctt tggtccgcag ataaaaaaag aacctttcac gactcgtgaa gggttctttt
     5521 tatgatttta ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag
     5581 gtacatctga agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg
     5641 tttcatattc gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg
     5701 cgaagagata ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac
     5761 cagaaccgta gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca
     5821 gtagagaagt tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt
     5881 aacggatgct ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt
     5941 ctaatacgct ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg
     6001 cctgataatc ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga
     6061 atgattcgat atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat
     6121 ctggacgcca gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata
     6181 aaatacgcgg gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac
     6241 caccgcttgc caagccgtaa cgagcaatat cacttgctat gactagtact tttcgttctg
     6301 gatgattttt gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct
     6361 cttttacttc aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg
     6421 cactcgcttt tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct
     6481 cacggtcttt ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt
     6541 catttgcacg attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat
     6601 catccccacg gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg
     6661 aaagacgatc aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact
     6721 ttactaaggt atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt
     6781 taaaatttaa tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga
     6841 atggtatatt tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa
     6901 gaacgccgat aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca
     6961 cacttgtgac taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag
     7021 taatatttgg aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt
     7081 tgaacagcgg tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg
     7141 gtatgaaagc agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga
     7201 tagccggcgg aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa
     7261 caaatgaata tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt
     7321 caaatgcaca tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag
     7381 aagagcagga tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg
     7441 gtgtattttc tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg
     7501 atgaagcagt tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct
     7561 cagaagaagg aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg
     7621 tgatccttgc atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca
     7681 aagaagtagc ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca
     7741 ttcaaaaatt gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca
     7801 atgaagcatt tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa
     7861 aggtcaatat atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac
     7921 gtatcttgac tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt
     7981 cattatgtat tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa
     8041 cacataagga tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc
     8101 aattaataga aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac
     8161 tttctgaaga gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa
     8221 tggggatcgc gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag
     8281 aggaaccatc tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg
     8341 cagaaacacc tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc
     8401 aagcagtgat caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa
     8461 gttatccttc aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca
     8521 tggggagttt ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg
     8581 gcgctaacat gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc
     8641 ctgaagaaga gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg
     8701 cctgttgtga aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg
     8761 ccaaaaaaat ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc
     8821 acaacaaagg gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac
     8881 gtgcggttag cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa
     8941 cagactggca gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg
     9001 ccacagtagg cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag
     9061 atattgattc agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc
     9121 tggctgcttt gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag
     9181 ctcgttcatt agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa
     9241 aattacgtga agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc
     9301 gggaaaaata aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt
     9361 tactgcagat cttgcttttt tctctatcag aaaatatgtt tattattaga attttttata
     9421 gttaaggtta ggcatcttaa gaaatcaggc aactccaaga tatcttcagg tagaagagaa
     9481 gtcgcctaac attgtaggat cataggtgat ttgttggaca ggtcaagctg atatttgagc
     9541 ctgatgttag aaggaatttc tttttacttg ctgtagaaat tattatatga ctaaaaaggc
     9601 tgggatttta aatcccagcc ttttatgaca aatagaattt ttatcgatca gtaatcgctg
     9661 tcgctgcaaa ttgttcgatc aaaggaatca aagtcaattt gactttgtat ttccgttcga
     9721 gtgctttttc gataacgaac tcattttcag tagtcataga aatagttgct aatttacgag
     9781 gttcatcagc atttttttca ttaataacta cttcatgttc attttgttct gtgtaaaatt
     9841 taggatactc agcaacggct tttgaaaaat cagcaaaact taaagcggca ttcgtatcag
     9901 ttgagcgtgt attttcagtt gtgtccgctt ttggtttttc cgttttttct tctggcacac
     9961 tgactgtttc ttctgtaact ggtgtcgcaa cagcttgcgc agttggttca tcgtttttca
    10021 atcgttgatc attccagtaa gacacgattt caataagaag attgtcacgg aatcgttcga
    10081 aaatcccgtt ttctcctgaa cgaattctct ttaatacagt attgaagttg tcatcgattg
    10141 gcacgttgat ccgtgtatct tcatttgttt tcccagctaa tccgatcgtt aagatttgac
    10201 ggtttttggg atcaagcatg acttgtaacg gcaaaccata acgtcgcaag ctctcttctt
    10261 ctagagaacg gaataattgt cctcttacac catccaatga aggacggaat tgatttaata
    10321 atttttgttt ctgttttttc ttcatatgtt ctctccttcg ctcataccct ttcgattata
    10381 agaaaaaaac agccaaatga aaaggagtaa tgcaaaaaaa cttgcgtata cgcaaattac
    10441 aacagaaaaa tttgcgtatt ttcccgtttt ttgaaagaaa aataaaaaat ggaacggatt
    10501 atgtatatat acataatccg ttccattctt tctatttttt cggtatcaag gcaaaaacag
    10561 gtagttcagg tgaataaatg acttcatgtt cgtgccaggg tttgaccatt agcgaatgat
    10621 cgaaccgttt tttcagttct tcgatgattt gatcatgtgt ccagtttctt gcaggaaaat
    10681 gttggatttt ctctgcatcg atcaaaaaga caaaatcgga atgaaaagtc tgggtaactt
    10741 cttccgagag agaattcaag cgttcgattg gatccataaa atcacctttc gacttaaaat
    10801 tgggtagtat ctgggtcttt tgctttgccg actacaccat ctaactgatc gatttgtttc
    10861 atttcttctt ctgttaattc aaaatcaaat aattcagtat tttctttgat tctgcttggt
    10921 gtcactgact taggaagagg aaggaattcg tgttgcagcg accaacgcaa agcaacttgt
    10981 gcaatcgtct tgtcatgtgc ttcagcgatt tgtttcattt caggcacatc gaagatttta
    11041 ccagtgccta atggactata tgcttcgagg atcatatcat gtttttttgc atactcaact
    11101 acagttgtct gtaattcgcc tggtgcaagg aaaacttgat tgaccatcgg cataattttt
    11161 gctgtttttg ctagtgtatc tagatggtgg ggtaagaaat tgctcacacc gattgctttg
    11221 attttacctg cttcatacag ttcttcaaat gctcgccatg tatctgcatt tgcttgttcc
    11281 caattatcac gaaaagcaac agggtttggc cagtggatca aaaacagatc taaataatct
    11341 gtttggagct tcttcaatga ttcttcaaaa gaagacatca caagttcata gctgtggttt
    11401 gcattccata attttgttgt caagaatatc tcttctctag gaattccact ttccttaatc
    11461 gcttggccga cgctttcttc attcttgtat ccttgggcag tatcgatatg gcgataacct
    11521 gcagccagcg cttctttaac agaggaaaca gcgacatccc catcaggtgt ttgccatgtg
    11581 ccgaatccta ctaccgggat ttcgtaacca tttgatagac gataagtgct tgttaatgaa
    11641 ttcatacttt cagctccttt tttattcgtt caactataag tgtagcgcat ttttcgaaaa
    11701 aaagaatcca ttttgtttct actcttgaaa tttttgttta aaagaaaatg attttgattc
    11761 tggtattttt gatttttttt ccgtaaacta agacagaagg gaatgagaaa atggatatct
    11821 atttaaaaaa agcaattctg catattattg acagagaaac tggttctcca gtcttttcac
    11881 aaaaagagct ggatctgaca aaagaatata ttcgtgattt tcttcaaaag aagatccaaa
    11941 aaatctcttc tgctcaaaca aaaactggac aactagcaga agaaagcatg atcgttgaat
    12001 cacttaaaat cgctcaagaa aattttgtgg aagcaagtga agcaatcgtt cagcggtggt
    12061 tcgatatcta ccaagaaagt gaagaagcac ctagtgcaga tgtatttgtt gtcttgtatg
    12121 aattagatac agtgatgtac ttatctttat tgaaagtcaa ttatcgtgag gcttacactc
    12181 actttgttga agcagacgaa gcagggatcg acaaccgctt gattttgaat cgagcaattc
    12241 taggggcgaa aacacaaaaa gcagatgaag cattcgctgt gaatttgact gattggacct
    12301 acgaattaat tgaaaaaaaa tatgagtttt ctggcaaaaa agagctttat ttttctggaa
    12361 gagtgatcga aagtgttcct gcaccttctt tagaagaaaa tgtaaaagtg atccaaaaag
    12421 tagccaagca tcttggaaaa aaatttgaga cagaagaatt tgatatcatg gctgatttga
    12481 aagaagctgt ctatgatacg atagaagaaa aaggtcggtt ggatcatgag atgattgcgg
    12541 aaaaagtctt caaggaaaac atcacggcaa aaatggcttt taaagaagaa gtacaagagc
    12601 aaggatttgt cccagaagca cctcctgtaa aagaagtaca agaaatttct gagaaaaaat
    12661 ttggaaaaca aaaattcaag ctatcaaacg ggatcgaact gatcgtacca gtagatgtat
    12721 atcgcaaccc agatttgatt gaatttgtga ataatccgga tggtacgata tccgtgatga
    12781 tcaaaaatgt ggatgaagta ttgaatcgtt tataaaaatg actagaatat taggtgttca
    12841 agtaccagtg atcagttaaa tgaattgtgt caagagaagg atcggaaaag acgaatataa
    12901 caaaaaaaga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    12961 aaacggcgga agcagaagca actccttctt atttcttgga gttaaacgct tctgtcccag
    13021 cctcttttta tctaataaaa ttttaagacc atttactaat tatcgatttt ttctgccgca
    13081 tttttaggca cttcagaagc ctttatttct tcccagctta atacaccttt acgaggattg
    13141 actttgagtt taagatacgc attcatcttt aatggatgtt ctctttcttc gcgaagcgtc
    13201 acctgttttt tttcaccatc tttattataa gccgcttgct tgtaggtata agtcgtgaat
    13261 ttctctccgt tatctgcttg ggtagctgac atttcgccat tcgtcgtgat ttttgtataa
    13321 taagcttcgc cgccgtaaaa ataatgataa gctgcaaaac ttccgccgag aaatatgcct
    13381 aaaacgacaa gtacgccaat taattttttc atgtacttca cctctttcct tattattaac
    13441 gaaatagaag atttcgtcta ttgaaagttg ttggcaacag ctgaatttta aacttttcct
    13501 cattttgttc ttattgcttc aaaagaaagt taaggactat cttgaagttt ttctcaaaaa
    13561 acacattttt ttatttataa aaggtacttt tttcgcgtat aagacagacg agtgctctta
    13621 gttgactttg accaatattg acgtataata agcttagttc gaatcttaca aaaagtaagt
    13681 cggacgtatt ctttttgatt tagtcaaaaa aagactataa taaaaaggtg agagctgaaa
    13741 tttttcagtg atctatatta tttaggaggt ttgtaaatta tggcaaaaga acattatgac
    13801 agaagtaaac cacacgttaa cattggtacg attggacacg ttgaccatgg gaaaactact
    13861 ttaactgctg cgatcactac agtattaggc aaaaaaggcc tagcaaatcc tcaagactat
    13921 gctagtattg atgcagctcc agaagaacgt gaacgtggga ttacgatcaa taccgcacac
    13981 gtagaatatg aaacagaaaa acgccactac gcgcatattg atgcaccagg acacgcggac
    14041 tatgtgaaaa acatgattac aggggcagca caaatggatg gtgcaatctt agttgtttct
    14101 gcaactgacg gtccgatgcc tcaaacacgt gaacacattt tattgtcacg ccaagttggt
    14161 gtaaaatacc tgattgtttt cttgaacaaa gttgatttag tcgatgatga agaattgatc
    14221 gatttggtag aaatggaagt tcgcgagtta ttgagcgaat atggtttccc aggcgatgac
    14281 actcctgtga tcaaaggttc cgcattaaaa gcattgcaag gcgatccaga tgctgaagct
    14341 gctattatgg aattgatgga tacagtagat gaatatatcc caacaccaga acgtgataca
    14401 gataaaccat tactattgcc agtggaagac gtcttctcaa ttacaggtcg aggaactgtt
    14461 gcctcaggtc gtattgatcg tggtgctgtt cgtgtcggtg atgaggtaga gatcgtaggg
    14521 atcaaacctg aaacacaaaa agcagttgta acaggtgtag aaatgttccg taaaacgtta
    14581 gattacgggg aagctgggga taacgtaggc gtgttgttac gggggatcca acgtgacgat
    14641 atcgaacgtg gacaagtact tgctaaacca ggttccatta ctccacatac aaaattcaaa
    14701 gcagaagtgt acgtgttgac aaaagaagaa ggtggacgtc atactccatt cttcaacaac
    14761 tatcgtccac agttctactt ccgcacaact gatgttacag gaacaatcac attgccagaa
    14821 gatacagaaa tggtcatgcc tggtgacaac gtgacgattg atgttgactt gatccatcca
    14881 atcgctgttg aaaacggaac gacattctca attcgtgaag gcggacggac agtaggttca
    14941 ggtatcgtta cagagatcga agcataagaa acgcgactcg aatattttgg taatgaaaaa
    15001 tgtttaaaat aaaagagcgg tccaaacagg atttctgttt ggaccgctct ttgttcttca
    15061 attgtataag aagagattgt agaagcatga actttcgttt tacttcagtc tcttttattc
    15121 atcatgagag tgttcatcta tcggtatgca gtttttaata ctttcttcgt atccattttt
    15181 tcaaaccact tattctacct tgtgtctgct caactggcgc attatcaata atcagcaaag
    15241 caatcataac atatgcaatc caactatctc tagaatatag agtataacgt gagacccagt
    15301 ttgtagcgta cttgctttta tattctctta atccatggaa agaataaaaa tgagaaccaa
    15361 actcgtaaac caagtatgca atcctttcct gtaaaaaact ctttctggaa gttccgacat
    15421 ttgaaagagg ggccatccct aaattgaacc attggatatg ttcttctttc atataggcaa
    15481 acaaatgcaa gaacaagaaa tccatactgc cagaaggtgc ttttttagaa tcatatcgca
    15541 tcagatcaat cgttcctact ttattgttcg tgtaagtagg catgatcgtt gcaaaagcga
    15601 caatttcttc ttgggtattt ttgacaactg caataggcgc tctttgtaga taatcttcag
    15661 agaaaaagcc taaagaaaac cctttttcct ttcgtgaacc caaccaatta tcagagatat
    15721 ttttgaaagt actcatttgc tcagtagaaa atggtggttg taagacatca aatgtaaatc
    15781 cttctcgttc gatccgattc aagacagcac gagtgccctt catttttttc cctgaagttg
    15841 taaaactatt caggtctaca taagcttctt cccccatctt aatgaaatcg tagccgaatt
    15901 catgcaaaat cataacaatc tcttcgcttg tttcgtagaa aactggtaaa tagcatagtc
    15961 gatctgtttc ttcgataaaa gcttcgattg cttctggaaa atcttctttc ttaccagaag
    16021 gatcacccat tacaatacat ttattattga agcaagcgaa ttggagaaga acagttggtt
    16081 caccgtcttt ttcataggca aacatccgct tatcttttaa gaagatcaat tgactatcgg
    16141 agtttcctcc ataagttgta aggatcttca gggccttttc ttcattaaag gcttcgccaa
    16201 tctgtttctt ttctccttga aggaaatgaa caaatagaac gatcatgaaa ctaacagcga
    16261 tgatggccaa caatcccgaa aaccaaatct tttcagaagg aaataagaaa aaggaaataa
    16321 aatggtgacg ccgatgaggg aaatctggca gattgtatac cccaataacg atgtaaagca
    16381 aggacagtgc accaataatg attccatcga tcgtacgcca ctcccatgaa tagatcaatt
    16441 gttcccgaaa taattcgttt ttagaagcaa gaatgatcag aagcagaata gacaagaaaa
    16501 ccaccggtgt gaaactaaaa tcacttaata aaacataaag taaagctaga acaatcaaga
    16561 agattgtagg caaatatgct cgtttcacac gtgctgcgat ccctctgccc ataatgatta
    16621 aaagaaatcc caatagtata ctaggaaatt ggataataaa atgaaatttc aatgggttca
    16681 aactatgaag ccagcgaaac tctgtaaaag cttgcgggat agtggcagaa agtactagca
    16741 tgattccaga aaagtataat aagacaacca cgattttatg cgcaatttct gttgctaatt
    16801 gtttagggat accagaataa cgctgatcaa aagtggaacc gatatttttg aagaaaaaga
    16861 cgattccaat caaaaacgga atgatgtagt agaacaggcg atagagcaag atccacacaa
    16921 cgactatttc tcttggcaca ccaatagcgg acaacccgat aatcatcatt acatcgaaac
    16981 tcccaatttc tcctggaatc atagacacga ttcctatcac tgaagcagca atgaataatg
    17041 gaattgtttg ccacagatca atccggattt ccattaaata accaacagaa aggaaactaa
    17101 acaggacacc ggtccattcc aacaatgaaa ctagcaatag ctgtccttga gttttaagtg
    17161 aggtaccacc aaggaatcct ttctttttga aagtagtaaa taaaaagacg acaggaaaat
    17221 atagaccgcc gccgatcaac catatccaat attgcttcaa aaaaggagca actggcgtga
    17281 agactacaag taaaaaagaa agcagactgt aaatagaaag tccagccatc aaaaacaaga
    17341 aaactttcga taatgcttgg atcacgtttc tgctctcttt cttttgtcca tatagttcag
    17401 agcgaagccc cacactaaca aacccgccga aaccggcaat attgttgatc gtattgatca
    17461 gccagcttgt ctcaaacagg taacgaggat tctgcttttg ttcaagcatc cgattcaaaa
    17521 taatatcata accaaccata ggtaacacag agataagacc aatcaatagc attagtccgc
    17581 ttttccatac aggtatagtg gcaaacgttt ctcctaattg ctggatagat agagttttac
    17641 cgatggacat caattcaccg acaataatga caatgacaga aatcaaaaag attgttttga
    17701 acagtcctag gtggtttttc agccattgta tggtattttt taacaaataa atcaccttct
    17761 attcctaata gttatgtttc agaaagaaat tgaagaactt ctcgattgaa gcgttcagga
    17821 tcttttctag ctaattcatg tccttgttct ttcacaagca caaaggaagc ttttggtata
    17881 gttttagcta tatacaaaga atgttttagc ttgattacat ctttttttcc cacgatgatc
    17941 aaagtggggg agttgatttt ttttaaatca ttttctgtaa gcccgatgtc atgcagcaaa
    18001 agtttgataa ctaaaagatt tttgcgaaga cttgggcgaa ataaagagag taaccatacc
    18061 catgcataat gaatattact gatgactctg gcagaaaaac gaacgccttt aacaagcgtg
    18121 tttccagaat ttaaaatcaa tcgatgaact tttttaggga agctgctggc aaataccagt
    18181 gctagatttg ctccatcact aaatccaaga aaatctgcct gatcgatttt ttccagcaac
    18241 atgatcgtat tcagatcctc ggccattaac tgaaaattca acatagatgc ttcgtttgtc
    18301 gaacgaccat gtcctcggct atctactaga tacacggtat agtagcgttc gagtacaggg
    18361 acctgttctg aaaaaaagcg gccgctgccg tcattgccat gcagtaaaaa gagagggaag
    18421 ccttgaccgc tcttttcata gtaaatttcg ctcccatctg gcatttttgc atatttcttt
    18481 tcttttttca cataatcacc tactgtgtaa agtataacga ttatatcagt cattgggcaa
    18541 ttgtttttac tgaaaaacaa cgatcatttt cgtaagatgt caaaaaatac aggaaaattc
    18601 attttttctt aaggcgtaca cagaaatcaa ggaagaaata gccaagaaga taaagaaata
    18661 gtaagataaa aaaagatgtc aaaataacta ggtgatgaaa tgaagcgaaa acgattatct
    18721 attatccgta ttctttttta catgatcatt ctgacggtta ttggtgcggt gttgttttta
    18781 ggaggaagaa cttatcgttc ggtaaaacga gtggaaagct atcaaataga aataacaaca
    18841 gctgcaaaaa attatcatat ggaaaactac gagaatttga tcaaggcgat aatattgacc
    18901 gaatcaaaag gaaaagggac agatctcatg cagagctcgg aaagtctgca tggagaagct
    18961 gagatcattg ataatccagc tgacagtatt gatcaaggtg tcaaatattt ggcggaaatg
    19021 atagtagaag caaagaaaca aggatgcgat ttatggacag ctgtccaagc atataatttt
    19081 gggaaagatt atattgccta tgtaaaaaat catggcggag aaaacacagt agaattagca
    19141 gaagcgtatt ctaaagatgt gctgagccca cttttaggaa atgaggaagg gaaacaatac
    19201 cgatattggc ggatccagtc cttgctttac aatggcggct atctctatca taatggaggt
    19261 aacatgtttt atcctgatat tgtcaaaatg aatgaaaaaa taatagagac ctatgaaaaa
    19321 gtctttaagt aaaatagcag gaaatgttca atcatgaata aaattaaatg aaagaaggag
    19381 tgcgcaaata gtggaaaaaa ggcaaaaaat ggaaaatcaa gaagtggggt acacaaccaa
    19441 agactggatg ttacggtttg tgaagggaat gtttatcggt tcaggattta tcttgccagg
    19501 agtcagcggt ggtgcattag cagctgtctt tggcatgtat gagcggctga tttctttttt
    19561 agcccacctt acaaaaaatt tcaaagaaaa tgttttgttt tttctgcctg ttggtctagg
    19621 cggagtgaca ggcatctttc tactttcttt tttagtcagt tttttattag gtgcgtatga
    19681 aacaacgata ttatggtttt ttgtcggatg tatcgctgga actgttcctg cgttatggaa
    19741 agaagcaggg aaaaaaggaa gagatacttc tgatgtgatc atcatggttg ttacttttgt
    19801 tctggcttat ttgtttttat tatacggcgc tcaattattt gacgggcaag tcgatcagaa
    19861 ttttggaaca tggctgattg ccggcggatt gattggtttg ggtatgatcg tcccaggatt
    19921 gagcccttct aattttttgg tgtatatggg attgtataaa gcgatggctg acggatttaa
    19981 agaagtacga cttgaagtga ttgtgccgat cggaattggt gggattcttt gtgttcttgg
    20041 attgtcaaaa atcatggatt acattttctc aaaggcatat agcaagctat tccatttcat
    20101 tttaggtgta gtttttgctt caacgattat gatcattcct accgattacg cgggattggg
    20161 agttgctggg gtactagctt gtcctgttct ttttatagcc ggtacagcac taggttggtg
    20221 gatgagccgt ttggaggaac aatataagta aagatagatt gataataaaa acagcacacg
    20281 ggtgatt
//
LOCUS       DASOUD010000047        20071 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.047, whole genome shotgun sequence.
ACCESSION   DASOUD010000047 DASOUD010000000
VERSION     DASOUD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.047"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            79..1239
                     /locus_tag="U4A29_001529"
     CDS             79..1239
                     /locus_tag="U4A29_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO0680658.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1255..1698
                     /locus_tag="U4A29_001530"
     CDS             1255..1698
                     /locus_tag="U4A29_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0680659.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(2181..2711)
                     /locus_tag="U4A29_001531"
     CDS             complement(2181..2711)
                     /locus_tag="U4A29_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEO0680660.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            2816..3367
                     /locus_tag="U4A29_001532"
     CDS             2816..3367
                     /locus_tag="U4A29_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO0680661.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(3520..3993)
                     /locus_tag="U4A29_001533"
     CDS             complement(3520..3993)
                     /locus_tag="U4A29_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO0680662.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            4131..5603
                     /locus_tag="U4A29_001534"
     CDS             4131..5603
                     /locus_tag="U4A29_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO0680663.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            5600..6310
                     /locus_tag="U4A29_001535"
     CDS             5600..6310
                     /locus_tag="U4A29_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO0680664.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            6662..6946
                     /locus_tag="U4A29_001536"
     CDS             6662..6946
                     /locus_tag="U4A29_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEO0680665.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(7227..7646)
                     /locus_tag="U4A29_001537"
     CDS             complement(7227..7646)
                     /locus_tag="U4A29_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEO0680666.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(7648..8232)
                     /locus_tag="U4A29_001538"
     CDS             complement(7648..8232)
                     /locus_tag="U4A29_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEO0680667.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(8279..9754)
                     /locus_tag="U4A29_001539"
     CDS             complement(8279..9754)
                     /locus_tag="U4A29_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEO0680668.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            9935..10246
                     /locus_tag="U4A29_001540"
     CDS             9935..10246
                     /locus_tag="U4A29_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680669.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(10361..12697)
                     /locus_tag="U4A29_001541"
     CDS             complement(10361..12697)
                     /locus_tag="U4A29_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0680670.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            12839..13507
                     /locus_tag="U4A29_001542"
     CDS             12839..13507
                     /locus_tag="U4A29_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0680671.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            13510..14553
                     /locus_tag="U4A29_001543"
     CDS             13510..14553
                     /locus_tag="U4A29_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO0680672.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(14790..15671)
                     /locus_tag="U4A29_001544"
     CDS             complement(14790..15671)
                     /locus_tag="U4A29_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO0680673.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            15830..16558
                     /locus_tag="U4A29_001545"
     CDS             15830..16558
                     /locus_tag="U4A29_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO0680674.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            16714..17031
                     /locus_tag="U4A29_001546"
     CDS             16714..17031
                     /locus_tag="U4A29_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO0680675.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            17024..17632
                     /locus_tag="U4A29_001547"
     CDS             17024..17632
                     /locus_tag="U4A29_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEO0680676.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            17687..18478
                     /locus_tag="U4A29_001548"
     CDS             17687..18478
                     /locus_tag="U4A29_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEO0680677.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            18616..19128
                     /locus_tag="U4A29_001549"
     CDS             18616..19128
                     /locus_tag="U4A29_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEO0680678.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            19154..20026
                     /locus_tag="U4A29_001550"
     CDS             19154..20026
                     /locus_tag="U4A29_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO0680679.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
BASE COUNT         6291 a         3519 c         4055 g         6206 t
ORIGIN      
        1 tcggctttca ttttctattt ttttcctgaa cccatataat aaaagaaaga acaaaaaaac
       61 ttgcaaggga ggacacatgt gaagcagagt aagcaaaaac agtctcgtcg acttttgttt
      121 cgtttgtttt tattaatatg tatcgtcgta gggttactga tcgctttata tccattttat
      181 gtggattctt taaatagttt tattgatcaa aaacgtatcg aacaagtaca aaaacggacc
      241 gcagctgaaa ttgaagtgca gcgaaagaaa atggaggaga aaaaacaacg attaactgac
      301 caaggattca atcctggtac tgatcctttc aatgaaaaga acagaaatga aagtacaaca
      361 tccagccaat tagaggaatg gctgatcgga agtatcaata tacctaagat ccaattaaat
      421 atttcacttt atgatcggct caatggtagg atccttgaaa atggagcagg cgtcttgcaa
      481 ggaacctctt tcccgcttgg tggaaattcg acgcattcag tgatctctgc tcacagcggt
      541 ttacctaatc gacggttatt taccgaatta gatcgtttag aaaacggaga cacgtttatt
      601 ttgactgttt tgggggagaa attggcttat caagttaaaa atatacaagt ggtactgcca
      661 gatgacacgt ctgttctaac gatttcagaa ggagaagact tagttacctt gcttacatgt
      721 acgccgtata tgatcaacac gcatcgtttg ctagtgacag gacatcggat tccttataac
      781 gaatcggtaa aaaaagaaga aggaaaagga aatcaggaaa gaaccatgcg gcaatggttt
      841 attcttgcag gaacgataat agctgtagtc atactgttag tgtttatcgg acgactaatc
      901 tatcagtata gattaagtaa aaaagtgctt gatttttcat ttattattag tgattcgtca
      961 gggaaaccgg taaatggtag ttcattcatt ttgaaacata agaaaaaaac acttactaga
     1021 aatggtgtgc cgttttcagt acaaagcgac catcatggga aagtcaagct ggatcaattg
     1081 cctggtggaa cttatcgaat cgtttcagct gatgatccta aagttgctgt ttcttttggt
     1141 attagaaaag taaaacaaga gaagatatac ttttttgagg ggcgaaaact tgtaaaagag
     1201 cttcaaaaaa atggttttta ttttaaaatc aatgaataag taaggagaga aaaaatgatc
     1261 cgcacaacaa caaaattgtc tgccaaagaa ctggaatctg ttctatcgat ttggctgcat
     1321 gctaacttgg attctcatga attcattcca tcaacttatt gggagaatca agtagaatct
     1381 gttaggaaag ctttaccaga tgcagtgatt tacttagcag aaatagatca agaaattatt
     1441 ggttttgctg gggtgattga tcagtttatc gcaggaatct ttgtgaaaag ggaataccgg
     1501 caacaaagaa tcggtacgca actaatgaaa aaaataaaag aaaccaataa aacactgact
     1561 ttgacagtct atcaaaaaaa tcaatcagct attcatttct atctttcaca aggttttaca
     1621 attcaaacac agcaaaaaga tgaagcagta aatgaggagg aacttgtgat gaaatggaat
     1681 caaaacgctg atttttgatg aacagaataa cgaaataatg ctcccagcct aattttttgc
     1741 tgggagcatt atttcatttt gaagtcacta atttaactgt gtgaatatgc agttgttaca
     1801 atctttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
     1861 tatgaggaac tatttttcca tttgtttctc taatactcag gtcaaaacgc tgatttcaca
     1921 acctcttgat aaacggtgtt caaacggctg cttctcggta ataagcccac tagcctagaa
     1981 aatatgaaaa gctattttct aactagcgtt cttattactc aaagcataac gctgatttca
     2041 caacctcttg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
     2101 aaacatgagg aactattttt ccgtttcttt ctctaatact caggtcagaa cgctgatttc
     2161 acaacctctc tttcacaacc ttattttact cgtttataat tttcatcgaa gtattttccg
     2221 ctacggatat catctggcat tccatcgtat ggttcttcgc taataacatc ttcattgtct
     2281 atacctgcat cacccatata agtgatcgta gcaaattcag gaacaagtaa ttttggataa
     2341 gtttcatatt tttcacgagc tgcttccatt tctgcgatat gttcgttttg gaaagtaaca
     2401 gtgatttctg gatgttcttc aacccattta gggaaaaaga tcattccgtg aagtttcttt
     2461 tcatttggca taaagtccaa tgccacgtct gtccctgttg gctctgtcca agtgattttg
     2521 aatacgccgt ccgtcaattt aacgatatca gcttcttggt cttttaccca acggcctttg
     2581 accattccgc catggatacg gtaatcaaca gtatggtcat ttttggcata ccattcatat
     2641 tcccaaccat tgtcatacgt atagataaag tgagttccaa taaaatcttc caattcttta
     2701 aattctttca tgtttaagca tctccttata aattaaaaca tgtaataaac tacatgtttt
     2761 cgattactca tttatgatag aatggattta aagatttgtc aaggagggaa aactgatggc
     2821 aagaaaaaat acacttcgat atattttgtt agggttatta agtaagaaga agatgacagg
     2881 atatgaattg aatcaatcgt tcaaaaatga aataggagaa ttttggcaag caaaacatag
     2941 tcagatttat cctgaacttg caaaaatgga agagcaagga atcattcagc atcaagtaga
     3001 gatcacaggt gaaaaattgg aaaagaaagt ttatgaatta acagaagaag ggaaggaaca
     3061 attggcagag tggattcata caccaaccaa tgaactaccg gttaatcgag acgaatttgt
     3121 tctgaaattg tatttcgtca aagacgtgaa tgatccggca ttagtagaaa ttattcagca
     3181 gcagcgtgac cttcatgaag aaaaacgact tcatctatta gagcgaaaaa aaacgatctt
     3241 tcctactgaa aaagaaaaac atgaaaatta cggacactac ctgattttag cgcatgcgat
     3301 caatcgggaa acagaatatg caaattggct aacagatgta ttagaagaaa gtaaccgaaa
     3361 aaaataaaaa aatatccgaa cagtgatgga gaattccttt ctatatgaaa gaaacgcaga
     3421 tcaccgttcg gatattctta tgttcaaaca ggcctttttt caagtattta tgtagcatct
     3481 acttattaaa aagcttggta cttaggagat tagtggcgtt tagagttctt ttaacaactg
     3541 ttcgtagtca gctttttcat cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt
     3601 gtcatgaatg atgtacatct tagcattctc ttctttgaag ttaccgattt gtaaaatcgt
     3661 atagcgttcg tcgatcaatt cgatatcttt gattgctttt gaaaaaactt tacggacatg
     3721 gtgcattttc ttttggcgga tttctgcctt ttctgcagct tttgacaaag taaatccgtc
     3781 atctaaatat tttttgatca gttctacttt tatgactgta ggtaaacgat attttctagg
     3841 acaattcgca tcagcttcga tcgaacgaat gaatcccttt tgttcccaat accttaattg
     3901 acgtggagac acacctacga tatcacttaa ttcactgatg ccaacaacca attgatcatt
     3961 ttccaacaaa cgtttaaggg aagccccaac catttttgtc actccttttg tcctcttgct
     4021 tcccttaatt atatccgtat taacttatgt gtcaagtagg ttgacaaata gcgaatttag
     4081 gactaaagta ttctagtagt ttcagaaaaa attcagaaag aaggagaaat atgagtaaca
     4141 accaacccgt agatatttac ggaaaaccat ataatcggac acttttagtt gttgtcttat
     4201 taattggtac attttgtacc gttttgaatc agaccttatt gacgacagct tttcctacat
     4261 tgatgaaagc tttcgatatt tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc
     4321 tagtcaacgg gatcatgatc ccaataacag catggctgat caataaattc agttcaagaa
     4381 atttatatct ttctgccatg acgatctttt tgatcggaac gattacttgc tttacagcac
     4441 ctaattttag tacattacta atcggtcgct tgatccaagc ttgcggcgtg ggagtatcga
     4501 tgccattatt gcaaaatatc atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa
     4561 tggggatgtc agggatcgtt attggacttg ctcctgcttt aggccctaca ttatctggtt
     4621 atatcattga taactaccat tggcgtgact tgtttggtat ggtactgcca atcgttgttc
     4681 ttgtcttggt tttagcattc ttcctaatga aaagtgttat tccattgtca aatcctagca
     4741 ttgatatttt atccgctatt ttatcaacga ttggattcgg tagtttactt tacggatttt
     4801 ctagtgtagg agatgacggc tggggaagct tgaaagtaat tggtttctta gttgttggtg
     4861 tgattttcat cgccttgttc tcatggcgtc aattacattt ggaacatcca ttcttggaac
     4921 ttcgtgtatt caaatcatct acattcacga ttgcagctgt tttagctggt gtaacaaata
     4981 tggcgatggt aggggcagaa atggtcgtac cattgtatat tcaaaatatt cgtggagaat
     5041 cagcattcca ttctggcttg atgttgttgc cgggtgcatt gatcatgggg gctatgatgc
     5101 ctgtgacggg tgcgattttc gataaatacg gcgcgaaacg tttagccatc acagggatgt
     5161 tcattttgac agcggcaact gcaccatttg ctttcttgac aaaaacgact ccagttttat
     5221 atatcgtgat cctttatgcg atccgtatgt ttgggatctc catggtcatg atgccggtaa
     5281 cgacttctgg tatgaatgca ttgccaaata acttgatcag tcatgggaca gcggtaaata
     5341 atacattccg tcaaatcgca agctcgattg gtacagctat tttgatcagt gtgttgacga
     5401 atgtgacaaa agcaaatatc ccaagcaaat ctgtattgga taatacgcca ttagcttata
     5461 aagatcaggc aatcaatgcc acactgtcag gttaccatgc cgcgtttatc gtagcgatca
     5521 tctttgggat tcttggcttt attattgcct ttttattgaa gaaaaaagaa cctgttctaa
     5581 tgaaagcggg ggataatgca tgattattgg agttttgatt gtcagcttgc ttgcttttgc
     5641 tttaacgaac gtgttcgcta aaaaaacatg gcaaaccttt ttatctttga ttttcggact
     5701 aatcttcgtc gcttcactaa gtttgatcgt cgctaatttg tccaatcact ttggaatgga
     5761 aaaagtaacg gaaacaaaaa cagaaaaaat tgtttccagt gctgattccc aaggagcaga
     5821 tatgcttttg tataaagctt tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa
     5881 tgaaaaacaa gaaaaaccaa aagcaactgg tacggataac gaaacaaata aagtagaaaa
     5941 gacagacggc gatgctgaaa aagtgacgaa aactacttat tgggaataca aaaacgacat
     6001 gtataaattc tggttcaata ttgctgataa caatcacgaa tatgacagcc gagtgaatac
     6061 attcaaaatc ccagagacat gggtagaatt aagtacggac caagcagcca agttagctga
     6121 attagtgaag aaacagcaaa gtacgatgga aagtgaagca aaagcttatg ttcaagacgg
     6181 tatggtaaaa gcaatgactg aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac
     6241 gaaagcctta gctgctgaat tccaacaaca agcattcgca aaattagtga aagaagcaaa
     6301 aggcgaataa tattttgaac accatgtagg aggttgtgaa aaagctgctc tgcatcaagc
     6361 agtaagaaca atcagataaa aatagcactc catatttttc atctgattgg gcttagtgcc
     6421 gtagatgcaa gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactct
     6481 gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt tgttgactat tgtgacttgt
     6541 gaccgaggag ttggcaggag aacaccgttt attcgaaaaa aggaactgcg acaagacttt
     6601 tgtcacagtt ccttcttttt tttgctatgc tatactagat gtaaaaaata ggagaaataa
     6661 aatgagggaa atttattctt ttgaatcaaa aatctatgct tccgaagtcg acaagggcgg
     6721 ggcatatatt atttttccgt atgatataag aaaagaattc ggtgctggaa gagttaaagt
     6781 ccaagcaact tttgacaatg tgccgtatga aggcagtatc gtgaatatgg gcgtgaaaaa
     6841 tccagatggt tcagtctgct acatccttgg actacgaaaa gatattcgta aaaacatcgg
     6901 aaaagaaatc ggtgatattg tagcagttac agtgaagcaa aaataaaaga ggttgtgaca
     6961 aaagacgtgt cacaacctct tgataaacgg tgttcaaaag ccagtttctc ggtagtaagt
     7021 caaatttatg caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg
     7081 aagcggcttt atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag
     7141 ctcaaaaacg gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca
     7201 taacgctgat ttcacaacct cttctttcat aatgtttttt caccatcttt ataataaact
     7261 ggttctttga tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct
     7321 tctgacatcg gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg
     7381 gaagtgatcg tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct
     7441 aagacatcgt ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct
     7501 gtatcacgag aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg
     7561 atcaataagt ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt
     7621 acttcaacta tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt
     7681 tacaattcca aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt
     7741 ttgaggctga ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa
     7801 tttagtcaag ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa
     7861 ctttcgttgc tccttcattg ttacatcagc agcacgtgca atcaggttgt cgcctaaacc
     7921 aacagagata cctgcgactg ttttcatact tgccggtacg atgatcattc catcggtcaa
     7981 aaatgatccg ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag
     8041 gctttcgaat tcagttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg
     8101 actgatgatt ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat
     8161 ggcataaatg gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc
     8221 caattgttcc attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt
     8281 atttcaaata tttttcccaa tcttctactt cttggaattt tgcacgtaca aaatgttctt
     8341 tcatatcgaa aggaactgta ccgtcaaaga tagtttttgc actcatccca cggaaacgaa
     8401 tagagccagg attatattct ggtaattctg atgggtccaa tggatggttc ctcattcctg
     8461 ggatcacgat gatatcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt
     8521 tcatgtcgaa aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg
     8581 ctgataaagc aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac
     8641 ggatttgcat gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta
     8701 cttttcctgg aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg
     8761 ccatagatac gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc
     8821 ggtgagtaac tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc
     8881 caggaaattc tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat
     8941 ttggcatgat ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg
     9001 ctataccatc aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat
     9061 tgtagccaag tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta
     9121 aaccaatatt gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt
     9181 gaccaatgtg gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa
     9241 ccatacgatg gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac
     9301 ccattgtgat gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc
     9361 gaatatcgaa gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt
     9421 tgtctacttt cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg
     9481 ttcgataatc atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt
     9541 tcacacgtgt gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag
     9601 tcggacgttt tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt
     9661 caggatcgat ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga
     9721 tgacttttct taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta
     9781 cctatctata gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc
     9841 tacaatggaa tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcactaa
     9901 aagaaagcgc atacttagtg ttggaggaag gaaagtgaaa aaaatgaagg agtcatttcc
     9961 taaagcagta aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga
    10021 agtaaaatac gtgaaatctc attggacaga agagataacc gatgccttgc aatttggaaa
    10081 aaaaggaaga ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac
    10141 tgaagtaacg attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc
    10201 agaagagttg tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa
    10261 aaacagcgcg aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca
    10321 gcgctcttat gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga
    10381 tagcggcgtc ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata
    10441 tagtagagac gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta
    10501 gatccgtcat gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca
    10561 cacctaatat cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga
    10621 tgtctttttt acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca
    10681 cagatgtata agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa
    10741 aagcaataag gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc
    10801 cagctaaatc agtataaatg attttatctt catctgattt gcctttattg taatcatcta
    10861 gataatccaa gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag
    10921 gcaagctgtc tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt
    10981 cagtagttaa gacatttaca ccactgtccc tttgagcttt gacgatttct gattctttgt
    11041 tttcgttgac gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat
    11101 tcattgtaga agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat
    11161 tctgatacat cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat
    11221 agtaagtatt gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt
    11281 tttcgtcttc tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat
    11341 tgccgtctac gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag
    11401 aataattgtc ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca
    11461 ctcccacacc tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat
    11521 tttgattcga gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc
    11581 ctgtagtacg ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat
    11641 cgacgtatgt ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg
    11701 cagtaatcgt tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac
    11761 gagtctggtt agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt
    11821 gcgtgttatc gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga
    11881 tgatcccaat actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat
    11941 tgttgaagga aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt
    12001 gatcatcttt tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa
    12061 attcaatgat ccgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca
    12121 ctaatttttc tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat
    12181 ctaacgctcc ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag
    12241 cgatggccac tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat
    12301 ctgtcaagcc gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa
    12361 caccacttaa tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt
    12421 tgtaactttg aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct
    12481 taaagtcttt agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc
    12541 caccaatcac attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga
    12601 attctttttg tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga
    12661 ctttataata tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa
    12721 atattcggta tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta
    12781 actgaagatg aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat
    12841 gaatcgtatt ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt
    12901 ggaacgagcg ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact
    12961 agaagcgaca cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta
    13021 tgacttgcta gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc
    13081 aaaggccgat tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac
    13141 aaaacccatc gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa
    13201 gatcaattac agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac
    13261 agtgttacaa gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa
    13321 attactgtct tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg
    13381 attagatacg aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg
    13441 ttttgaaaag acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt
    13501 tatttaaaga tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc
    13561 ttcatttcga tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga
    13621 cattctatct cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt
    13681 gcggggttca gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg
    13741 catccaatca gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt
    13801 cgaatggatg aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt
    13861 atggtccaag aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc
    13921 catgaattca aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca
    13981 aatctctctg acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag
    14041 ctttcacaat taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa
    14101 ctggaaaaga aagagtatcg attagatgaa caaatcagag aagtaatcgt ttttttgcag
    14161 cctaaatggg agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca
    14221 ggcaatgaag agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac
    14281 aatcaaatca atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg
    14341 gaagtgaccg attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa
    14401 ttttatcaag gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt
    14461 aaaaaaattc tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga
    14521 acgacagcaa tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc
    14581 tattgaaggt ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg
    14641 ttcttttggc ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca
    14701 ttgaaacaag gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg
    14761 ggtggataga tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa
    14821 aatgtatctg aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct
    14881 gttgttggag aaatagggag aatcacagta ttttctttta atagattctt gactgttttt
    14941 tccataacaa tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt
    15001 gctcctttat aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt
    15061 tgatacaagt tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga
    15121 tccaatttct ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct
    15181 gtttcagtag gcaaccactc aatcgaactt ggtggggcct ggaaatttct agcaaagtgg
    15241 atcgtgtcac tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc
    15301 aactggactg taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta
    15361 taattgatca ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag
    15421 gtttcaagca cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc
    15481 aataagcttc cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg
    15541 tctaattctg tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt
    15601 cgatcagcag ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta
    15661 tggatatcca tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg
    15721 aattggtgta atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag
    15781 aaatttttct gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg
    15841 agttttgatc aatgtaggtt ccgttttgct tggcggacta attggcggat tagtaggaaa
    15901 taaattaagt gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg
    15961 gatgggaatc tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt
    16021 gataggaaca gcgattggtc tgattgttca tttaggtaat gggataaata agggggctgc
    16081 gctgatgcaa gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga
    16141 tgaatttatc agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat
    16201 ctatggaagt ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt
    16261 ccttgatttt ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat
    16321 agcaattccc caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc
    16381 attgactaca ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc
    16441 taccggtttt agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat
    16501 gatcgttatc atgccactga gctggatgtg gacaaattgg attatgccac tgttataata
    16561 aaaatcaaag agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt
    16621 tttgtatttg acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat
    16681 aaaatattac acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt
    16741 cttgatggta ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca
    16801 aaaatcgtac aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga
    16861 ttgaaaaaag aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga
    16921 tattactcgt tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt
    16981 gtttttcgag attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa
    17041 tgaagttgtg gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta
    17101 ttatgaggaa tattttttag attctggaaa aacattagat cagatcattg atgaatatgg
    17161 tacaccaaag cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc
    17221 agaaaacgag ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat
    17281 cggtttattt gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg
    17341 gggactattg gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat
    17401 cttaggtgtt ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct
    17461 gagtggatta ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc
    17521 gccactgttg ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg
    17581 gatgggacga cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac
    17641 acataaattg agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg
    17701 cctcctagca ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg
    17761 tcctcggttg atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt
    17821 catccatgca gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac
    17881 gacttatgat aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa
    17941 tgcagtcaaa aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac
    18001 tattgttgtc cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc
    18061 aattgatatc aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt
    18121 atcgttcaat gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg
    18181 attagatgag gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct
    18241 agaagacagt ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt
    18301 gcatgagatg gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa
    18361 agactaccag ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac
    18421 tgctaaagac acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc
    18481 ctgtactcaa ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt
    18541 ttcatcgctt aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac
    18601 agggtgaaag agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg
    18661 gaaagattac atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa
    18721 aaagtgatgg ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat
    18781 gaagttttca aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt
    18841 atcgatacag gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt
    18901 cgggaagatt tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt
    18961 acagaagaaa agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg
    19021 acacaggcaa ggggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg
    19081 gcgcgtattc gaaaaaaaat taaaaaagta acattagata tttattgaag gataaaggag
    19141 cgcaggaagt agcatgagag aaaaaagagc attttatatc aatggttatg cagggattgt
    19201 cggattggtc gtattagctt tagttggatt gtttttcttc tatttaggta tgtggcaagc
    19261 gaaagtttgg gcactttttt taagtatttt tctttggttg atcacgttat tgttgttaag
    19321 ttcggcaact gtagtcagtc ctaatcaagc aaaagtaatt ttgttttttg gacaatattt
    19381 aggtacgatt cgagaaaatg gctttttcct tacgatacct ttggcacaaa aaatgactgt
    19441 ttcgttgaaa gttcggaatt tcaatagttc tgttttaaaa gtcaatgatt tggatggaaa
    19501 ccccatcgaa atctcagcag tagtagtctt taaagtcgtt gatacagcaa aggcattatt
    19561 cgatgtagcg tattatcaag attttgtaga aatccaaagt gagacagcca ttcggcatat
    19621 cgctagccaa tacccgtatg atacattcaa tgagaatgat ttgacgcttc gaggaaacac
    19681 gacggcagtg tctgacgagt tgcaaaaaga gctgcaagag cgtttagcag tagcaggtgt
    19741 cgaagtaatc gaaacacgct tgaaccattt agcctatgca acagaaatcg ctagtgccat
    19801 gctccaacgt caacaagcaa aagccatttt gtctgctcga caaaccattg ttgaaggagc
    19861 tgtcacaatc actcaaatgg ccttagagca aatcgaagat ggacaagaaa tcaatttcac
    19921 cgatgatcgg aaagtccaat tgatcaataa tttacttgtc tcaatcatta cagacaaagg
    19981 aacgcaacct gttatcaata caggcgacat gaaagaccgt aagtgacata ctttttgtaa
    20041 gataggaaga aggagtttag gacggattat t
//
LOCUS       DASOUD010000048        19614 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.048, whole genome shotgun sequence.
ACCESSION   DASOUD010000048 DASOUD010000000
VERSION     DASOUD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.048"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(330..692)
                     /locus_tag="U4A29_001551"
     CDS             complement(330..692)
                     /locus_tag="U4A29_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680680.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="U4A29_001552"
     CDS             complement(766..1146)
                     /locus_tag="U4A29_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO0680681.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1210..2403
                     /locus_tag="U4A29_001553"
     CDS             1210..2403
                     /locus_tag="U4A29_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO0680682.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2480..3064)
                     /locus_tag="U4A29_001554"
     CDS             complement(2480..3064)
                     /locus_tag="U4A29_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO0680683.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="U4A29_001555"
     CDS             3265..4263
                     /locus_tag="U4A29_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO0680684.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="U4A29_001556"
     CDS             4375..4884
                     /locus_tag="U4A29_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEO0680685.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="U4A29_001557"
     CDS             complement(4914..5573)
                     /locus_tag="U4A29_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO0680686.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="U4A29_001558"
     CDS             complement(5575..6339)
                     /locus_tag="U4A29_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO0680687.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="U4A29_001559"
     CDS             complement(6347..7024)
                     /locus_tag="U4A29_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEO0680688.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="U4A29_001560"
     CDS             complement(7061..8446)
                     /locus_tag="U4A29_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO0680689.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="U4A29_001561"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U4A29_001561"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="U4A29_001562"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="U4A29_001562"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEO0680690.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="U4A29_001563"
     CDS             9385..10242
                     /locus_tag="U4A29_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0680691.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="U4A29_001564"
     CDS             complement(10323..10658)
                     /locus_tag="U4A29_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680692.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="U4A29_001565"
     CDS             complement(10725..12095)
                     /locus_tag="U4A29_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEO0680693.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..12709)
                     /locus_tag="U4A29_001566"
     CDS             complement(12173..12709)
                     /locus_tag="U4A29_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO0680694.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13018..14499
                     /locus_tag="U4A29_001567"
     CDS             13018..14499
                     /locus_tag="U4A29_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0680695.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14602..15309)
                     /locus_tag="U4A29_001568"
     CDS             complement(14602..15309)
                     /locus_tag="U4A29_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEO0680696.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15388..15726)
                     /locus_tag="U4A29_001569"
     CDS             complement(15388..15726)
                     /locus_tag="U4A29_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO0680697.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15837..16799)
                     /locus_tag="U4A29_001570"
     CDS             complement(15837..16799)
                     /locus_tag="U4A29_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO0680698.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16814..18145)
                     /locus_tag="U4A29_001571"
     CDS             complement(16814..18145)
                     /locus_tag="U4A29_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO0680699.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18153..18833)
                     /locus_tag="U4A29_001572"
     CDS             complement(18153..18833)
                     /locus_tag="U4A29_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEO0680700.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19052..19531)
                     /locus_tag="U4A29_001573"
     CDS             complement(19052..19531)
                     /locus_tag="U4A29_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO0680701.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         5852 a         4033 c         3245 g         6484 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcactatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt cttacctccg
    12721 tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat tattgataat
    12781 tatcaaaaca tatttagtta tatccattaa aaatcaattt ttccgcctta aaaacaagaa
    12841 tatcttgaca aaatacccta ggaaataaac aatagaataa acaaggtaac aaaaaaatta
    12901 aataacgtta tataaaaaag aaggcgacac tctgtgagaa taacacagac tgaaatgact
    12961 ttccactcac agtttttgtc taactatata caaaaacaga ataaaaagga ggtatttatg
    13021 ctaattgaag caagaaaaaa agagttactt gttctacaaa tggtcattca aaaaaataat
    13081 tgttctttac aagaattatc caatgactta catattccaa aacggacaat caaagaactg
    13141 atccgaaaaa taaatgtatc actcaagcaa tttttattga ttgatgaatt tatttattct
    13201 aatcataaag gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt
    13261 tctaagttaa aattatgcta cctaaaagaa tccaatcgtt ttaactattt gatgttacta
    13321 atcaattatc cacaaactgc tgtaccaaaa aaatatttac ttgaacaact ttatatatcg
    13381 ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa attccattta
    13441 cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc tatccgtctc
    13501 tatctttact tttttctttc agatacattt ggaggtaacg attggccatt ttcctcactt
    13561 gaattaaatg cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa
    13621 gaaaaaaata tctacttgct gttaacttta ttcatattac gaaacaaaca acaggcatat
    13681 tttccaaaaa cttcttctga aatcactgaa ttgatggact tattgcagca tacacatgat
    13741 tattcagaat actttacttc tttttcagtg agtcgccctt gtgaagagac agaaacagag
    13801 agagcctact ttaattattt tttctattgt ttttttccaa atcaaatctc ttttcgacaa
    13861 aaacaggaga ttggtttttt attttccaca agcaaaaacc ttatttgtcg acaagtcggt
    13921 caattgatga cttacttaac tcaacagttt cctctcaacc atttagaaaa taaaatacca
    13981 atttattttt attttcttgt gttggcagtt gtttttttcg aactgttggg tgaatccagc
    14041 agtagttttt ctacactttg ctttccagaa gccacgtcca atcattctag ttcttttata
    14101 caggaaaaga aattaagaga aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac
    14161 caaatcagtc aataccttag tcaattgatc caacacttac tacaaacaga aacgaaagag
    14221 cagttactca tccatttgca gataaccaaa cagtttacag gaaattatca tcttcaaagc
    14281 catttgcagc agttatttcg caaagatgct gttaaaatta ccgcatattc agaacatgct
    14341 gatttgatca ttacagatca gcatgagaaa atttcagatg aaacggagac tttctatctg
    14401 gatacaacgc acaatacagt gttatttggc aagttaataa aagcggttca tgaactgtat
    14461 atgaaaaaag gttatgagaa caaaacaaag aggatgtgac aaaattcttg tcacatcctc
    14521 ttattccaaa taaatggtat tcaatcagct aactctcggt attagctcag atcaaagcgc
    14581 tgatttcaca acttctttat tttaatctct tcggttgccg ccgaacaata agatcaagcg
    14641 caacagctgc aggaatgtcg aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa
    14701 tacgtgtctt gccatcggta cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg
    14761 gattgctcgc cgagacgcgt taaattcgac tggtaatgtc accaattgga aaagtaatgc
    14821 taaagaaaaa gcgaaaatcc cgatattgat taacgtagag ttccagctaa gcaatacacc
    14881 taccataatc aaggggaacg aaatcatcga accaaagtta gctgctggaa caagtgctgc
    14941 acgaatcttc aatgggacat aacccgaagc atcttgtacc gcatgtccgc attcatgagc
    15001 tgctacacct atcgcagcaa ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat
    15061 cttattacct gaattgtagt tatctgtgag atctccggca atttgttgaa caccgacatt
    15121 atttatctgt tgactttgta atatatattg tgctgcttgt gtaccagtca cattgttttt
    15181 gcttctcact tgatcgtatt ttcggaaagt actattgacg tacgctgacg caattccgga
    15241 aataactaat ccaagaatga ccaaaagaaa tgtcggatct aaaaaaccat acattccata
    15301 accatacatt ttcgtttcac tccttttcgt ttactttatt ttatcattag tagtaggaaa
    15361 agaatgtgaa aagaccttga atattcttta attctttttc acaaattccg atttcagttt
    15421 catcggtcca aatccttcta ccttgcaatc gatattatga tcgccttcta ctaaacggat
    15481 gttcttgact tttgttcctt gtttaatggc acctgacgca ccttttactt tcaagtcttt
    15541 aatcactgtc acgctatcgc cctctaacag acggtttcca tttgcatcta ataccactaa
    15601 tccgtcttct atgacagttt cttgttcttc tttgttccat tcgttcccgc attctgggca
    15661 aatcaacaag cctcgatctt catatgtgta ctctgagcca cattctgggc aatttggtaa
    15721 tgtcatcttc tttctccttt aaaatcttta gtgagcacta ttgtaacgga aaaagaagat
    15781 cttgaaaaga tacatttgtg atttttacta caaaaggcac gttttcgaac gaagtatcat
    15841 tctgtttttc tgctcttata cgattggcat atatcttgga tctctttata gatttcctct
    15901 acttctgcta aatcttttgc attcgcacca cttgctaatg gatggcctcc tccatgatgg
    15961 cgtttagcca attcattgat gatcggcccc ttagaacgta aacgtacgcg gtaataccct
    16021 gaaggctgct gaacaaaaat cccccaagcc agcacttcgt caatcactcc aggaagtgaa
    16081 acgatcgaag ctgtctctga atcatcgatt ccatatttat caagaatttc ttgcggaagg
    16141 attattcttc ctgcaccatt ttcatccacc tggatatttt gataaagata accggaaagt
    16201 ttagcgactt tcatcggcat ttgttcaatc tctctgttca aagcacttgc atcaaatgga
    16261 taatttctca atatcgaagc aatctctaat gtacgggacg tcgtggaggg atacaaaaag
    16321 cgccctgtat ctccgacgat tcctgcataa agtaaccgtg cagccttaac gttcatcttt
    16381 aaagattcag gaaacatcaa agcaaattct gcaatcatct cactacagct acttgctttt
    16441 gtatttaccc acacaagatc tccataaggg tcatcattgg gatgatggtc gattttgatc
    16501 agtttttctg ccaactgata tctttgatca ctgattctgg gtgaattagc tgtatcagtc
    16561 acgatgacta gcgctccttg gtagaagaca tcttcaactt cttgcatttc tgctaaaaag
    16621 cgaagccctt caatcgtttc acctacttga cgtatttctt tttctgggaa actttcccgt
    16681 aatagttcgg ctaatcctac ttgtgatcct aatgcatcag gatcagggcg caaatgcctg
    16741 tggataatga ttttatcgta tttttttatt gtatctacta tttcttgctg tactgtcatt
    16801 tcactcctgc tttctaagaa cgttccatta cttgacaaac aacgattgct ttggaaacga
    16861 tggtgttttc aataaacact tcgatatcca gtttagcgga acgtctgccg atttccaata
    16921 cacgtggacg aatgtctaat tcgctttcca attggatcag ccgcaaataa tgtaggttca
    16981 cttgttcaat caagatattt cgtttttggt tcatcaccat tgttttttgt gcgacatcag
    17041 aaatgatttc gctaagtacg ccaaaagaaa tggttccaac gccattgacc atttgtggtg
    17101 ctactacgaa tttgaactta gggttttcca aacgatttcc ttcacggtca gtatcaatcg
    17161 tatggatatt tccagatatc tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga
    17221 caagctgcat cgctttcatt acatcttgcc gggtaatcaa gccttccaat gtcaagtcat
    17281 cggaaacgac cggcatgact tctagcccat cccagatcat ttgatggcta acagaggcaa
    17341 tactcattgt tttctttaca ctgattggtt ctttcgtcat cacccgatcg atgatctggt
    17401 ttggcgattt ccctaaaaca tcttttgccg taacaatccc catcaaacga tgatggcggt
    17461 tgacgactgg aaaacgcgaa tgctgcgttt cttctgacaa acgctgataa tcagaaatcg
    17521 tatctgtctg ctgcaaataa tgagtctttt cagctggcat atagatatca ctgacaagca
    17581 tgatatcttt tttgatcaat tggtcgctta acgctcggtt gatcatggtt gcgactgtaa
    17641 atgtatcata agaggtgcgt aagatcggca tttcaagatc atcagctagc tttgcaatct
    17701 cagcagtcgt attgaatcct cctgtgatca atactgcggc gccatcttcc aatgctaatt
    17761 tttggacttc cgcccggttg ccgacgatca ttagtgatcc tggcgtaata tagcgttcca
    17821 tggcatgttt agtcatcgcg ccgatgacga atttattcaa gaccttgtct aatcctgccg
    17881 ccccgcctaa aacgtcccct tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa
    17941 tatgtttttt taatttgcgt tcgatacgga tggtccctac tcgttgaatt gttgaaacta
    18001 gtccgacatt ttctgcatct ttgattgcac ggtacgctgt tccttcactg acattcaatt
    18061 cttttgcgat gcttcttaca gaaattcgat tgccaacagg taaactttca atatattcta
    18121 aaatcaaatc atgttttgtc gccattttct gcctacactt ccagcgtttc cgagacgctc
    18181 attatttttc cttcactatc ttccagtaac tctacaaaag attcgggatc ttgttcaatg
    18241 acagggaaag tattgtaatg gattggaact actttcttgg cacgaattgt ttgcgcagcc
    18301 tttgctgcat cttctggccc catcgtatag ttatcgccaa tcggcaagaa agcgacatca
    18361 atatcgaatt cttctgcaag caatcctaga tcactgaaat aagcagtatc ccctgcatga
    18421 taaatcgttt ttccttctgc ttgaataata attccgctag gctcgcccat atactgaacg
    18481 ataccgtcta cttcatagct tgaactatgc tgtgcatgta cgaattttac cacgccgaat
    18541 ggaaacgcat gttttccacc gatattcata ccgaatgaat ctactcctct gctgttcgca
    18601 taatcagcaa tctcaacaat actgataatt gtggcttgat tttcttttgc aataggaatc
    18661 atatctccta tgtggtcact atgaccatga gtaatcagca accaatctgt ctttacttct
    18721 tctgcagtta agtctgtcaa tggatttcct gtaataaacg ggtcaatcat cacgcgttgg
    18781 ccattttcca tttcaattgt taaacaggaa tgtccatgat acgtaatttt catattttac
    18841 acctcgcgtt ttctctgtat cttttttatt aaaaacgatt tcatcacttg cacatcaatg
    18901 aaaaaatata gctgctaaat atattttcat tggattgata gtacagtatg tacaaagcaa
    18961 ttgtaacaga taatacagga tgcaccaagt accaagcaga tataatctca agtttgaggt
    19021 ctctgctctg cttagcttct cttctcgtcc cttaactaat taaatcagcg taaataggat
    19081 ggacgatctt ttctaaatcg gctactttta tccgaaggct tctcccgatt tcaccgcttg
    19141 acacaattac ttcttctttt tcttttaaag aactgtctgc taaaaaacaa taatcaggat
    19201 gtgcgagttt tattccaata ggggtgtttg ctccgtgggg atatcctgta tgcattaata
    19261 caaaatccat tccaggaaaa ccaactttac gatctttcgt cacttgtctc gtttttttgt
    19321 aatctaagtg atcgttcaaa ggaatcaatg cgatgatcac cccagttttg tttcctttca
    19381 ctacgagagt tttacatatc aaagctggat caatccctct tttttgtagt tcttctgtga
    19441 aagattcttc taccacatga ctaaaatcaa gtggttgata gctgatcttt tgttcttcta
    19501 aataacgttc gacttttgtt tgactcatca ttgattcact ccctttattc tatcgtttat
    19561 tataaagcga gggatgctgt attcaaaaat tatttgtttt tcgcaaaaaa agag
//
LOCUS       DASOUD010000049        19457 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.049, whole genome shotgun sequence.
ACCESSION   DASOUD010000049 DASOUD010000000
VERSION     DASOUD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.049"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..3660
                     /locus_tag="U4A29_001574"
     CDS             133..3660
                     /locus_tag="U4A29_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEO0680702.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="U4A29_001575"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="U4A29_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO0680703.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="U4A29_001576"
     CDS             7448..7789
                     /locus_tag="U4A29_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0680704.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="U4A29_001577"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="U4A29_001577"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO0680705.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="U4A29_001578"
     CDS             9146..11566
                     /locus_tag="U4A29_001578"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO0680706.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U4A29_001579"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U4A29_001579"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO0680707.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="U4A29_001580"
     CDS             13000..13737
                     /locus_tag="U4A29_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO0680708.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="U4A29_001581"
     CDS             13751..14566
                     /locus_tag="U4A29_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO0680709.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="U4A29_001582"
     CDS             14579..15226
                     /locus_tag="U4A29_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO0680710.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="U4A29_001583"
     CDS             15242..15898
                     /locus_tag="U4A29_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO0680711.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="U4A29_001584"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="U4A29_001584"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO0680712.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="U4A29_001585"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="U4A29_001585"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEO0680713.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDSEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="U4A29_001586"
     CDS             18240..18758
                     /locus_tag="U4A29_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO0680714.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="U4A29_001587"
     CDS             18865..19365
                     /locus_tag="U4A29_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO0680715.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6542 a         3341 c         4177 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatagtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DASOUD010000050        18866 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.050, whole genome shotgun sequence.
ACCESSION   DASOUD010000050 DASOUD010000000
VERSION     DASOUD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.050"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            334..1357
                     /locus_tag="U4A29_001588"
                     /pseudo
     CDS             334..1357
                     /locus_tag="U4A29_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1700..1956
                     /locus_tag="U4A29_001589"
                     /pseudo
     CDS             1700..1956
                     /locus_tag="U4A29_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2343..2570
                     /locus_tag="U4A29_001590"
     CDS             2343..2570
                     /locus_tag="U4A29_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO0680716.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2586..3752
                     /locus_tag="U4A29_001591"
     CDS             2586..3752
                     /locus_tag="U4A29_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO0680717.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3987..5030
                     /gene="hrcA"
                     /locus_tag="U4A29_001592"
     CDS             3987..5030
                     /gene="hrcA"
                     /locus_tag="U4A29_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO0680718.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            5124..5687
                     /gene="grpE"
                     /locus_tag="U4A29_001593"
     CDS             5124..5687
                     /gene="grpE"
                     /locus_tag="U4A29_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO0680719.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5738..7567
                     /gene="dnaK"
                     /locus_tag="U4A29_001594"
     CDS             5738..7567
                     /gene="dnaK"
                     /locus_tag="U4A29_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO0680720.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7718..8884
                     /gene="dnaJ"
                     /locus_tag="U4A29_001595"
     CDS             7718..8884
                     /gene="dnaJ"
                     /locus_tag="U4A29_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO0680721.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(9252..9989)
                     /locus_tag="U4A29_001596"
     CDS             complement(9252..9989)
                     /locus_tag="U4A29_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO0680722.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9991..11493)
                     /locus_tag="U4A29_001597"
     CDS             complement(9991..11493)
                     /locus_tag="U4A29_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO0680723.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11752..13746
                     /gene="uvrB"
                     /locus_tag="U4A29_001598"
     CDS             11752..13746
                     /gene="uvrB"
                     /locus_tag="U4A29_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEO0680724.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13756..16572
                     /gene="uvrA"
                     /locus_tag="U4A29_001599"
     CDS             13756..16572
                     /gene="uvrA"
                     /locus_tag="U4A29_001599"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO0680725.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16835..18100
                     /locus_tag="U4A29_001600"
     CDS             16835..18100
                     /locus_tag="U4A29_001600"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEO0680726.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            18104..18835
                     /locus_tag="U4A29_001601"
     CDS             18104..18835
                     /locus_tag="U4A29_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEO0680727.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
BASE COUNT         6321 a         3184 c         4036 g         5325 t
ORIGIN      
        1 actttttcaa aatttctaaa tcattcaata tgtagcgctt actaaattat tcgccataaa
       61 attttataat tggatcactc attgttttac ctaagtcata tttaagtaat aaccggtgtt
      121 tttacataag aagacttcgg gtatttttta gtttgattag ctctgatata cctataaaga
      181 atatgctaag gtaagaatag taaaaattca catagatgag gtataataat gatattttta
      241 tatattacag ataacttgac acaaaatgag tttttttcga tttttcaagt tatctaatgc
      301 aattatattg atatcattag gagctgcatt gttatgaatc caacattgat tactaagaaa
      361 gaattactaa aaaaattaga tatttctact ggtgtattag ctaatttaat tagaaatggc
      421 atgccaaaag agggagaaat gtttaatctt gataagatta ttacatggcg agaaaattgg
      481 tctaaaaaca tattaggtga gttagaagtt ggtagagtat atactaacaa agaaatttca
      541 gagaagttta aatgttccaa acagggtgga atgagaagat ctcatcaaac taatactctt
      601 gtactgttta gtgatcaaac aggaagtaat gtttataaag ataagtggtt aaatggtatt
      661 ttacaatata ccggtatggg gctaaaaggg gatcaagtat tggataagaa tcaaaataaa
      721 gttttagcca attcaaaaag taattttgta aagattcatt tatttgaaac atttaaacca
      781 aaagaacaca catatctggg agaagtatat ttagcaggac aaatatatac tgttaatgaa
      841 aaagatagtt cgggcaacag cagaaaagta tataaatttc cattagcttt aataaatcaa
      901 gagcaattga ttgaagataa agatatataa ccaggaagaa aatcaaacta gacatattcg
      961 taatttatct aatgctaaat tagaggaaga agcaagaaaa gttagtaatt ataataaaaa
     1021 aatggctgca aaatatgtta acgaagagga gttttgcttc tcaaaaacaa aaatatataa
     1081 acgtaatccc ttcgtatctg aatatgttaa acgacttgca aaaggtattt gtcaactttg
     1141 ccaagaaaaa gggccattta taaaagacgg tgtaccttat ttacattgtc atcatataga
     1201 atatttgtcg caaggtggaa aagatgtgat tgaaaactgt atcgcgttat gtcccaattg
     1261 tcatgctaga attcatgaac ttgagttacc tgacgataaa gaaaaattgc tgaagatcgt
     1321 tgaaaaaaga gaaaatagct tttctaaaat accgtaattt atttttatag gacaaataag
     1381 ctaatgtgag ctatgccttc tatataagac taagttctat tataagtaac gaagtaattc
     1441 tcgttccttc ctgtagtaat attttttaaa taacctatag gcttcttcaa aagtacatcc
     1501 tccagtaaat tctaagtact ggaggattct ttttctagtg ttgctttact catgaaaaag
     1561 tagttggcta cttcatcaaa atttctgaac ttttttaatg tataataaag agctcgatca
     1621 tttactacaa ggatgctagc tcctacattt tcacagtttt ttagcaagta aatttttcta
     1681 aaatcacatc aggaaatgca tgagattctt taagagaggc aattataggg ctatgattac
     1741 ggttcgtgta agtgacgcca aaaaagaatg gctttcttat atggctgaat tttatgggat
     1801 tactttatct gaacttctaa aaaactattc gatggaacaa ctagaagatg aatacaatcg
     1861 tcaaacggct gaaattgccc ataaacattg gataagaagt gggaaaacaa cagtattcat
     1921 ggaagaatcc ttaatgaatt tggtgggctg gaatgacctg ccgtttaaaa ttcacgcccg
     1981 aaattcagaa gcagctctga ttggcaaata cgtgtattgg tagagataga agataatcaa
     2041 ttcgttgtta tcgctattca agtagggcac cgaagaaatg tctatgatta agagactgtt
     2101 tatttttaca aaaaaataat aattggaatc tagttaaaac tcttgaagtg agagatttga
     2161 ctagattttt ttatgaataa aataaggaca aaattgttta ggaagaatca tgagtgtaaa
     2221 aaaatcatcc aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt
     2281 agtatgcgta tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat
     2341 ctgtgaaaat agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg
     2401 cgattaccgt ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag
     2461 catttttaga gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc
     2521 ttgaacgcga ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa
     2581 tttcaatgat agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc
     2641 cattttgcga gcatatctgc tattactgtg actttaataa agttttttta gaagggcagc
     2701 cagtagatga gtatgtggaa atgctgctga atgaaatgag attaatgctg gaaagtcatc
     2761 ctacagcgaa tttggaaaca ctttatgtag gaggcggaac accaacttct ttatctgcta
     2821 aacaacttga tcgactatta tcaggtgcta gagagatcct gccttttcaa aatggtaatg
     2881 agtttactgt agaagcgaat cctggtgatt tgacccgtga aaaacttcaa gtgatgaaaa
     2941 actatggcgt caatcgttta tccatgggag tgcagacatt tgacaatcgc ttattgaaaa
     3001 aaattggacg taaacatact gcagaagatg tgtatcaaac aatgcaattt ttagaagcag
     3061 aaaattttac caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag
     3121 gatatcggga tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact
     3181 cattgatcct cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac
     3241 tgcctgatca ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc
     3301 atggacgtca ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata
     3361 acctcatgta ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc
     3421 ttggagatat tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag
     3481 caaatgaacg cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag
     3541 aaatgttctt aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct
     3601 atcaaaaaac aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt
     3661 tacttgttaa tgaagaagac cgtgtttatt tgacaccaag agggactttt ttggggaaca
     3721 atgtgtttga gcgcttttta tttgaaaaat agtttgaaga atatttgttt tttatcgaaa
     3781 cttgaaaaga aaaagtttag aaagtttgac aatgaggtat tgaagtattg atataactgg
     3841 atttacatca attatttaga attagcactc ttgtgtttag agtgctaaaa aatggagtct
     3901 ttctcttgac aggagaaggg cttcttgcta tatttataag tgaattagca cttagaatat
     3961 atgagtgcta atgagaggtg aggaagatgt taacaaagcg acaaaacgac attttacgtc
     4021 tgatcatcca acactatacg agtagtggcg tgcccgttgg ttccaaaaca ttaatgtctg
     4081 agggagtcaa ggccagctca gcaacgattc gcaatgatat gaaagcatta gaagatgaag
     4141 gattgctgtt gaagactcac tcttcttctg ggcggattcc atctgcttta ggctatcgtt
     4201 attacgtgga tcatttgtta aaacctgctc gcgtagcaga agatgatttg ctgctgattc
     4261 gtcagtctct aggcagagaa tttcatgaaa tcaatgagat tattaagcaa tctgctgaga
     4321 tattatctga gctgacaagc tatacgactt tttcattggg acctgaaata aaagaccgcc
     4381 gactgactgg ctttcgtatc gtacccttga ataatcgaca gattattgct atcgtcgtaa
     4441 cagatatggg gaatgtagaa agccaagtgt tcactcttcc cgaatatatg ggaagtcagg
     4501 aactagaaaa aatggtacag attgtaaacg accgtctagt cggagatcct ctgatgacgg
     4561 tgtaccataa acttcgtacg gagattccga tgattcttca taaatatttc cagacgacag
     4621 aagggatgtc tcagttattt gacaccgttc ttggtcatgt ttttgaagaa aaggtttatg
     4681 tcagtggaca aatgaatctc ttggattatg agccccgtca agatcttgat cagttcaaat
     4741 cgatgttttc ttttatgaaa gactcagacg gacttacaca aatgatcact ccgatggata
     4801 gcaatattca tatcaagatt ggtgctgaat taggcaatga tttattgcag aatatgagta
     4861 tgattcaagc aagttacgag atagatggtc atggaagagg tacaatagcc ttgttagggc
     4921 ctactagtat gccttattct cggatgttcg gtctagtcga tgtttatcga agagaactag
     4981 cagctaaact tgctgattac taccgctcgc ttgacttgtc aaactcgtaa tgtgaataac
     5041 gaagctattt gcagctgatc gttctctctt tcttagtaag agcaatcaaa cagctgactg
     5101 aataatgaaa ggaagactgt atcgtgaaag aaaatcaaga agaactagat aaagaaatga
     5161 cagatgctca gccagaaccg gaaatcgatg tcgaagctgc agaagattcg ggtatttcag
     5221 aagctgaagc ggaagaattc gaaacagcga aattaaaagc tgagttagaa gaaatggaag
     5281 ataaatactt gcgggctcgt gctgaaatcg caaatatggc aaatcgagga aaaaatgagc
     5341 gtgagcaatt acaaaaatac cgttcacaag atttagccaa aaaattactt ccatcaattg
     5401 acaacctgga acgtgcatta gccacagaag tgtcagatga tcaaggggca ggtttgaaaa
     5461 aaggcgtgga aatggtcttg gaaagtttgc gtaacgcact ggaagaagaa ggaattgaaa
     5521 aaatccctgc taaaggagaa gcctttgatc ctaatcttca tcaagcagta caaactgttc
     5581 cagcgactga agatacacca gccgatacga ttgtagaagt actacaagaa ggctacaaat
     5641 tacatgatcg cgtgttaaga ccaactatgg tcattgttgc acaataattg tgcggatata
     5701 aaacttacac tttcaattaa agaaggagac gttaattatg agtaaaatta tcggtattga
     5761 cttaggtacg actaactctg cagtagcagt attggaagga aacgaagcaa aaatcatcgc
     5821 taacccagaa ggaaaccgta caacaccttc tgttgtatca ttcaaaaatg gagaaatcca
     5881 agtaggggaa gttgcaaaac gtcaagctgt gacaaaccca aatacgattt catcaatcaa
     5941 acgccatatc ggtgaagcag gttacaaagt agatgtagaa ggtaaatcat atacaccaca
     6001 agaaatctca gcaatgatcc ttcaatatat caaaggattc gcagaagact atttaggtga
     6061 aaaagtcgaa aaagcagtta tcactgtgcc agcttacttt aatgatgcac aacgtcaagc
     6121 aacaaaagat gctggtaaga tcgctggtct agaagtagaa cgtatcgtca atgaaccaac
     6181 tgcagctgct cttgcctatg gtttagataa aacagatcgc gacgaaaaaa tcttagtatt
     6241 cgaccttggt ggcggtactt ttgacgtatc tatccttgaa ctaggagacg gtgtctttga
     6301 tgtattgtca acagctggcg acaaccatct aggtggggac gactttgata acaaaatcat
     6361 cgactacatg gtagcagaat tcaaaaaaga aaacagcatt gacttgtctc aagacaaaat
     6421 ggctttacaa cgcttgaaag acgctgctga aaaagcgaaa aaagatttat ctggcgtatc
     6481 aagcacacaa atcagcttgc catttatcac agcaggagaa tctggaccat tgcatttgga
     6541 aatgacattg acacgtgcaa aatttgatga attaacagct gacttagtag aacgtacaaa
     6601 agtaccagta cgtcaagcgt taaaagatgc aggtctgtca caatcagaaa tcgacgaagt
     6661 tattttagtc ggtggatcta ctcgtatccc tgcagttgta gaagcagtac gtaaagaaac
     6721 aggaaaagaa ccaaacaaat cagttaaccc tgatgaagta gttgctatgg gtgctgctat
     6781 ccaaggtggc gtgatcactg gtgatgtgaa agatgttgtt ttacttgacg ttacaccatt
     6841 gtcattaggt atcgaaacaa tgggtggagt gttcactaaa ttgattgacc gcaatacaac
     6901 gattccaaca agtaaatcac aagtattctc aactgcggca gataatcaac cagcagtaga
     6961 catccatgta ttacaaggtg aacgcccaat ggctgctgac aacaaaactc taggaagatt
     7021 ccaattgaca gatattccag cagctcctcg tggtattcca caaatcgaag taacattcga
     7081 tattgataaa aacgggattg taaatgtatc tgcgaaagat ctaggtacac aaaaagaaca
     7141 aaaaatcacg atcaaatctt cttcaggtct aacagacgaa gaaatcgaac gtatggtgaa
     7201 agatgcagaa gcaaacgctg aagcagataa agctcgtaaa gaggaagttg acctacgtaa
     7261 tgatgtagac gcattgctat tctctgtgga caaaacattg aaagaattag aaggcaaagt
     7321 ggacgaagaa gaagtgaaaa aagcagaaac agctcgtgac gaattaaaag ccgctgttga
     7381 agcaaacaac atcgaggaaa tgaaaactaa acgcgatgca ttgaacgaaa tcgttcaaaa
     7441 cttgactgtt aaactatacg aacaagctgc acaacaacaa gctcaagaaa atccagaagc
     7501 agctcaaggc ggagcagatg atgtcgttga tgctgatttt gaagaagtcg atggcgacga
     7561 taaataatca gttcactgaa tttcgagaga aggccaaagt tgctactttg gctttttctt
     7621 ttgaaaaata gtttgaagta tcggtttttg tttcaaacaa aatatgatat gataacaagc
     7681 gacgtgaaaa tgatgagaac caataggagg aaagcctatg gcaacaaaac gtgattatta
     7741 tgaagtctta gggctctcaa aaggagcatc agaagatgaa ataaagaaag cttatcgtaa
     7801 gctttcgaaa aaataccatc ctgacatcaa taaagaacct gatgcggaag aaaaattcaa
     7861 agaagtatca gaagcatatg agattttaag tgacccgcaa aaacgcgctg catacgacca
     7921 atacgggcat gcaggcacag atccaaatta cggtgctggt ggagctggtg gcggatttgg
     7981 tggattcggc ggcttctctg gtggcggctt cggtggtttt gaagatatct tcgactcttt
     8041 ctttggagga ggcggtcggt cagtcgatcc aaatgcgcct cgtcaaggtg ctgatttgca
     8101 atatacggtc gacttgtctt ttgaagaagc gatttttggt gtagaaaaag aaatcaagta
     8161 taatcgggaa gaaatctgtc atacttgtgg tggaaacggt gcaaaaccag gcacacaacc
     8221 aactacttgt cataaatgtc atggtgcagg tacgatcaat gtagaacgcc aaacgccact
     8281 tggacgagtg atgagccgtc aaacttgtga tgtttgtcat ggcacaggga aagaaatcaa
     8341 agaaccttgt ccaacctgtc atggctcagg acatgagaag aaagcacatt cagttaaagt
     8401 gaatgtacca gctggtgtag aagaaggaca acaaatgcgt ttagctggtc aaggagaagc
     8461 tggagaaaac ggcggaccat ttggtgattt gtatgtggtt tttcgtgtag aagaaagtga
     8521 tatctttgat cgtgaaggtt ctgaaattta ctatgaattg cctttaagtt ttgtacaagc
     8581 agctttgggg gatgaagtcc aagtaccaac tgtccatgga gatgtgaaat tgaagatccc
     8641 tgctggaaca cagacaggta cgaacttccg tttacgtgga aaaggcgctc cgaagttacg
     8701 tggtggtgca acaggcgacc aacatgtcaa agtaaaactg atcacaccga aaaatctaaa
     8761 tgaacaacaa agagaagcat tacgtgcatt cgctgaagct ggcggcatca aagttgaaga
     8821 acagcaagaa gacggtttct tcgataagtt taaagatgct tttggcggga aaaagcggaa
     8881 gtagtttttc tagtaaaaat ataaagtaga cttgcccaaa ttttctattt ctagtaaaaa
     8941 cgctaagtca ttaataatta aaaaccagac gaacaatcat acgttcgcct ggtttttttg
     9001 tagttgtcat gtttgtacca acaaaaaagc cagctttaga ataagataat catcatagtt
     9061 tctgaaggtg gtatcgacta tgtacttgat atcgtcaatg aaccgatggt actttcttat
     9121 gcgatggaac aggtagagcg attgcttgtt tgtgcaggag aacaagcgat gcggctggta
     9181 cagttaagtg aaaaaacaag aaactaaaaa tgcccggtcg tcatgaagcg accgggcatt
     9241 tttggttttt attaaatatt taatacttta ttcaagaaat cttttgtccg ttcattttgc
     9301 ggattttcaa agatttgttg aggcgttccg tcttctaaaa agtttccgtc gtcaatgaac
     9361 atgacacgat tggccacttc tttagcgaag cccatttcgt gcgtaacgat aaccatcgtc
     9421 ataccttgtt ctgctaactt tttcatgacg ttcaagacat caccgaccat ttcggggtcg
     9481 agtgcagatg taggctcatc aaaaagcatg atgtcagggt tcatcgctag agcacgagca
     9541 atagccacac gctgcttttg tccaccggaa agcatttcag gatacatatc ttttttatct
     9601 tctagaccaa ctgtctgcag caatttgatt gcttttttct ctgcttcttc tctagattct
     9661 ttcttcaggt ccatcggtgc taaaatgatg ttttccaaaa tagaaagatg agggaatagg
     9721 ttaaagtgct ggaataccat tccgatatgt tgtctcacca agttgatatc tgtgctttta
     9781 tccatcaagt gggcgccgtc aataataatt tcaccgtcag aaggttcttc taaacgattc
     9841 aagcaacgca aaaatgtact ttttcctgaa cctgaagggc caatgataca gacgacatct
     9901 ccttcgttga tggacacatt gatgtcgttt aatactgtgt tatcgccgta ttttttgact
     9961 aaatgctcaa ctaagatttt ttctgccatc ttacttcacc ttcttttcta aaacttttgc
    10021 taattttgtc aatgctgtga tcaaaatcaa gtacatgatc gcaatgatca agtaaacata
    10081 cgtactttgt gttgtacgtg caacaatgat cttaccagtt tgcagcaatt cgactacacc
    10141 gattgcggaa atgatcgttg tgtcttttaa tgagataacg aactgattga cgaatgatgg
    10201 gatcatgatg cgtatcgctt gcggcaaaat gattttttgc attgtccgat tataagaaag
    10261 acctaaactt ctggaagctt ccatttgtcc gacaggaacg gcgttgatcc cgcctcgaac
    10321 aatctctgcg atataagcac ttgcgttaag agtcaaggtg ataacaccag caaggaagtc
    10381 aggaatcgta aatccaacga taccaggcaa cccaaagaaa ataaagaacg caagaaccat
    10441 cattggaatt cctcggatga tatcgacata aatactagca attgtgcgaa gagcgcgtat
    10501 tggtgctaca ctgaataatc caaaaatcac accgattact atagccaaag caaaggagat
    10561 caaagttagg agaattgttc tccaaagtcc tttcaacaat gcttgatagt tgttttggat
    10621 aagtccaaga aatgttgatt catcagcagt ggaagactca ctggaagttt ctgaaccatc
    10681 cgctacatat tctttaataa tcttatcgta ttcgcctgtt ctttttaatt ctttcaatcc
    10741 ttcattgaac atattaagaa gctcaggatt ttgtcctttt ttaacggcaa atgcatattt
    10801 tccgccagat tcttttttga tcggtgtttc caattcttgt ccttgtttga tcgcataacc
    10861 gattaccgga taatcatcca tcattgcatc gattgcgcca acttttaacc cgtcgtacaa
    10921 ttgatcagca gcatcaaaat atttgataga atagccatat tcagattcat ggtcgcttaa
    10981 aaattcagca ctttcggtac caacttttgc tccgactgtt tttcctttca aatcttcata
    11041 agacttgatc gaagtatctc cttttttgac agctaattgg atcccgctat caaagtaaga
    11101 atcagaaaaa tcatagcttt tctttcgttc atctgttaca gtcattccgg caatcattcc
    11161 gtctgcttgt cctgattcca aagcttgcaa agcagcagag aaaccacggt agtccattcg
    11221 gatgttaaat ccttgcagtt tggcgatgtt ttccatcaat tttacatcga tgccttcgta
    11281 ctcgcccttt gcgttttgaa attcaaatgg agcaaaagca gagtcactgg caattacgta
    11341 tacgtctttt ttgggctcta ttttttgcat tgtctcgctt gtgcttgact ctgccgttgt
    11401 agtgttgtca gcttgtgcaa catgtccaaa cgcaagtgtc acaagtcctg tcattaagaa
    11461 aacaaatgag tgaaataaat gctttctttt cataatcaac ctcctaaatt tccaatattt
    11521 tattattata ggcgaaaagt gctgaatcag acaagactgt aagctcgaaa aaatgaaaaa
    11581 aatgcgagtt tctaacattt tatgttataa aacgtaacat gcataataag ataaaaaagt
    11641 ggcgaaagta tgttcggtgt ggtataattt tttagtaagg aatgcctttg ctttcctttt
    11701 ctttttattg gtcgtaaaat gaaaaggaat gaatgaaaag gaggacgaag aatgattgag
    11761 agagatacct cccgacactt tgagcttgtc tcgaaatacc aaccggctgg tgatcagcct
    11821 gaagcgatta accaattagt ggatggcgta gtcggcggga aaaaagcaca aatcttatta
    11881 ggtgcgactg gaacagggaa aacatatacg atttccaatc ttattgagaa agtgaataaa
    11941 ccaacactaa ttattgccca taacaaaaca ttggctggtc agctgtacgg ggaattcaag
    12001 gaatttttcc ctaacaatgc tgttgaatat tttgtttctt attatgatta ttatcagcct
    12061 gaagcttatg tgccttcaag cgatacgtat atcgaaaaag attcaagtgt aaatgatgag
    12121 atcgacaaat tacgacattc ggctactagt tcattattag aaagaaatga tgtgatcgtc
    12181 attgcatccg tttcatgtat ttttggatta ggttcgcctt ttgagtacca aaaacaagta
    12241 gtttcgattc gacagggagc agaactagat cgcaatcagt tgattcgtga cttagtcagt
    12301 atccaattcg aaagaaatga tatagatttt cagcgcggtc gttttcgagt gcgcggtgat
    12361 gtcgtggaga tttttccggc ttcaagagat gaacgtgcct tacgtgtgga gttttttgga
    12421 gatgaggtcg aacgtatccg agaagtcaat gcactgacag gtgaagtttt aggagaaacg
    12481 gaacacgtgg caatcttccc ggcgactcac tttgtgacga atgatgaaca tatggagcat
    12541 gccgtagcga atatcaaagc ggaattagaa cagcgtttaa ctgtcttgcg aaatgaaaac
    12601 aaacttctgg aagctcaacg tttagaacag cgaaccaatt acgatattga aatgatgtta
    12661 gaaatgggct acacgtcggg aattgaaaat tattctcggc atatggacgg acgtaaagaa
    12721 ggtgaaccgc cttatacgct attggatttc tttcctgaag attttttgat cgtggcggac
    12781 gaatcgcacg taaccatgcc tcaagtcaga ggaatgtata atggtgaccg agcgagaaaa
    12841 cagatgctag tagattacgg tttccgtttg ccatctgctt tagacaatcg gccacttcgg
    12901 ttagaagagt ttgaaaaaca tgtcaatcag atcatttatg tctctgcgac gccaggacca
    12961 tatgaacatg aacaaacaga taccgttatc caacaaatca tccgtccgac agggctgctt
    13021 gacccagtga tcgaagttcg tccaatcatg ggacaaatcg atgatctagt aggcgaaata
    13081 aatgaacgag tcgaaaaaga ccagcgggtg tttgtaacga cattgacgaa aaaaatggca
    13141 gaagacctca cagattattt caaagaatta gggatcaaag tcaaatattt gcatagtgat
    13201 atcaaaacgc tggaacgaac agaaatcatt cgagatcttc gattaggtga atttgatgtg
    13261 ttagttggaa tcaatttgct tcgtgaagga ttagacgtac ctgaagtttc ccttgttgct
    13321 atcttggatg cggataaaga aggcttttta cgtagtgaac gttccttagt ccaaacgatt
    13381 ggacgagctg ctcggaatga agaaggtaaa gtaatcatgt acgcagacaa agtaacagat
    13441 tctatgcgtc tagcgatgga tgaaacatca agacgccgta cgattcagca gaagtacaat
    13501 gaagaacatg ggatcgttcc aaaaacgatc attaaagaaa tccgagattt gatttctatt
    13561 acgaaagaat cggaagatga tacaaaagaa gcggtacaag tctcgtatga agaaatgacg
    13621 aaagaagaaa aagatacgtt attgatgaaa ttagaaaaag aaatgaaaaa tgcggcgaaa
    13681 gcattggatt ttgaaacagc cgctaatgtg cgagatatga ttttagaatt aaaagcttca
    13741 aagtaggagg acaacatggc taacgataaa attacgattc acggtgcacg tgcccataac
    13801 ttgaaaaata tagatgtaac gattccacga gataaattcg tggtcgttac aggtttgtcc
    13861 ggttctggaa aaagttccct agcatttgat acgctgtacg ctgaagggca gcggagatac
    13921 gtagagagcc tttctgctta tgctcgtcag tttttaggac aaatggataa gccggacgta
    13981 gatagcatcg acggattgag tccggcgatt tctattgatc agaaaacaac aagtaaaaat
    14041 cctcggtcga cagtaggtac tgtgacggaa atcaatgact atttacgctt gttgtttgca
    14101 cgtgtaggac atccgatttg tcccaacgat catatcgaga ttaccagtca atcagtagat
    14161 caaatggcag atcaagtaat ggctttgcct gagcgcacaa agatccaagt gttggcaccg
    14221 atcgtggtaa agaaaaaagg acaacataaa aaaatattcg aacgaatcca aaaagaggga
    14281 tacgtccgcg ttcgagtcga tggtgagatc tatgatttaa gtgaagtgcc tgagctggag
    14341 aaaaacaaaa aacatgatat cgcgatcgtc atcgaccgga tcattgtgaa agaagggatt
    14401 cgttctcgtc tatttgattc tttagaggcg gctctgcgat tggcagacgg ctatgctctg
    14461 gttgacgtga tcggtgaaga agaaatgttg tttagcgaac actatgcctg tccttattgc
    14521 ggatttactg ttggcgaact agaaccaaga ctgttctcat tcaatgcgcc ttttggagct
    14581 tgtccagact gtgatggttt aggtgtcaaa ttagaagtcg atcaagattt agtgattcca
    14641 gatagaacga agacacttag tcaaggtgct atcgttccat ggaatccaat cagctctcag
    14701 tattatcctc aaatgttagc gcaggcttgt gaaagtttcg gtatcgattt agatacacca
    14761 ttcgaagaat taccggaaga gcatcaggat atcatcttga atgggacaga tgatcttttc
    14821 catttccatt atgaaaatga ttttggtggc gttcgcgatg tagaaacgac tttcgaaggg
    14881 gtattgaaga atatcgagcg tcgttatcat gatacgaaca gtgatttcac gcgtgagcaa
    14941 atgcgtcttt atatgacaga attaacttgt cagacatgta aaggttatcg attgaatccc
    15001 caagctctat ctgttcaaat caatggaacg aatatcgggc aaacaaatga gttgtctatt
    15061 caaaaagcag tagatttctt ctcaaactta acgttatctg aacaagagaa agtaattgcc
    15121 aaaccgattt taaaagaagt aaatgacaga ctttcattct tggaaaatgt gggactagac
    15181 tatctaactt tgagtcgcgc ttccggaaca ttatctgggg gagaagcgca gcgaatcaga
    15241 ttggctacac aaattggatc gaatctttct ggagttttgt atattttaga tgaaccatcg
    15301 atcggattgc atcaacgcga caacgatcga ctgattggct cattaaagaa aatgcgtgat
    15361 cttggcaaca ccctcgttgt agtcgaacat gatgaagata cgatgtgggc agcagattat
    15421 ctgattgacg tcggacctgg ggccggagaa caaggcggac aaatcgtggc tgcaggcact
    15481 cctgaagaag tagctaatga tgaaaattct ttgacaggga aatatctttc agggaaaaaa
    15541 tcaatccctg taccaaaaga acgccgtaaa ggtaatggga aagcaatcaa gatcactggt
    15601 gcttcagaaa acaatctgaa aaatattagt gttgaatttc cattaggaga atttgtagca
    15661 gtcacaggtg tgtctggttc tggaaagagt acattggtca atagtatctt aaagaaatcg
    15721 ttagcgcaaa aattaaataa gaattctgct aagccaggta aattcaagac aatttccggc
    15781 tacgaaagta tcgaaaagat catcgatatc gatcaaagcc caatcggccg gacgccgaga
    15841 agtaatccag cgacttatac aagtgtattt gatgatatcc gtgggttatt tgctcaaacg
    15901 aacgaggcaa aaatgcgggg ttataagaaa gggcgtttta gtttcaacgt aaaaggcggt
    15961 cgttgtgaag cttgtcgcgg ggatggaatt attaagatcg aaatgcactt tttgcctgat
    16021 gtctatgttc cttgtgaagt atgtcatggc aaacgatata actctgaaac attagaagtg
    16081 cattacaaag gaaaaagcat tgctgatatt ttggaaatga cagtagaaga tgctgtagaa
    16141 ttcttcaagc acattccaaa gattcatcgc aaactgcaaa cgattgttga tgttggctta
    16201 ggttatgtga cgatggggca accagcaacg acattgtccg gtggtgaggc acaacggatg
    16261 aaacttgcca gtgaattgca caaaatctct aatggaaaga atttctatat actagatgaa
    16321 ccaacgacag gacttcatag cgatgacatc gcccgcttgt tgcatgtatt acaaagatta
    16381 gtagatgctg gtaacacagt tttagtgatt gaacacaatc tagatgtaat caaaacagca
    16441 gattatatca ttgatttagg accagaaggt ggagaaggtg gaggaacgat ccttgcgact
    16501 ggaacaccag aagaaatcat taacgtaaaa gaaagttata caggtcacta tttgaaaaaa
    16561 ataatggtat aaagaggata gatagctgag atagttaaaa aaacataaaa aaatgaattt
    16621 ttgcttgttt tattaatatc agtgttctag aatcattaca tggtagttct ttctacactg
    16681 attggtatag acaaaaactt cctataagtt caatccgttc aaaaaagaat tgagcaatcg
    16741 gaggagtttc ctttgattgc ttttttcttt atggtatgat attccttgtg tagttggaaa
    16801 agaaaaacaa gagattatga gtaagtgaga gataatgatg aacagtattg aaaatgaaga
    16861 atttatccct atattattag gaagcgacat gaacgtttat ggtatggcac gttcttttaa
    16921 tgaagcatac ggcaaaatct gccaagcata tgcttcggat cagttagccc ctacccgcta
    16981 cagtaaaatt gtcaatgtag aagttattcc aggatttgat aaagaccctg tgtttatcga
    17041 gacgatgctt cgtttggcaa aagaaagata ttctgataag agtaaaaaat atcttttgat
    17101 tgcttgtggt gatggatatg cagaattgat ctcccaacat aaacaagagc tgtcagaata
    17161 ttttatctgt ccatatattg attattcttt atttgaaaga ctgatcaata aagtaagctt
    17221 ttatgaagtt tgtgaagaat atgatcttcc gtatcctaaa acattgatcg tcagagaaga
    17281 aatgttagta aatggacatt tggaacagga attgcctttt gaattcccag ttgctttgaa
    17341 acctgcaaac agtgtggagt atctttctgt ccaatttgaa ggaagaaaaa aagcttttat
    17401 tttagagact agagaagagt tcgatttaat tttaggccgg atatacgaag ccggttataa
    17461 aagtgaaatg attgtacaag attttatccc gggtgatgat agtaatatgc gtgtgctaaa
    17521 tgcgtatgta gatgaagatc atcaagttcg gatgatgtgt cttggtcatc cactgttaga
    17581 agatcctaca ccagcatcga ttggtaatta cgttgtgatc atgccagatt ataacgaaaa
    17641 aatctatcaa accattaagg catttttgga aaaaatcgaa tatacaggct ttgccaattt
    17701 cgatatgaag tatgatccta gagatggaga atataaatta tttgaaatca atttgagaca
    17761 aggtcgtagc agtttctttg tgacattgaa cggcttgaat ctggctcgtt tcgtcacaga
    17821 agatcgagtg tttaacaaac cctttgttga aacgacctat ggaacgaatc aatcagataa
    17881 agcaagatta tggatgggtg tgcccaaaaa aatcttttta gaatatgctc gggaaaatga
    17941 agacaaaaaa ctagctgagc aaatgatcaa agaaaatcgt tacggtacta ctgtgtttta
    18001 tgaaaaagac cggtcaatca agcgctggtt gttgatgaaa tatatgttcc ataactatat
    18061 tccaagattc aaaaagtatt ttcatgtgaa agaaggctag ttcatggaga attttttcag
    18121 tattttaggc ggaatgggca cgatggcgac agaaagtttt gttcgcttga tcaaccaccg
    18181 aacgaaagct acaaaagatc aagaatattt gaattatgtc ttattcaatc atgcaacagt
    18241 tcctgatcgt acagcttata ttttagatcg atcagaagaa aatccaatgc cgtttctgct
    18301 ggatgatatt gagaaacaaa atttattgca gccgaacttc attgtgttga cttgtaacac
    18361 cgcacactac ttttttgaag aattacaagc ggcaacggat attcctatcc ttcatatgcc
    18421 aagagaagca gcaaatgaac ttgtccgtca gcatacgaca gggagagtag cgattctagg
    18481 aacagaagga agtatgaaag ctggaatcta tgaacgcgaa gtcaaaaatc ttggattcga
    18541 aacagtgatt cctgatactg ttcttcaaga aaaaatcaat tatctgatct atcatgagat
    18601 caaggaatcg gattatttga atcaagagct atattacgaa atcttagaag aagcggtaga
    18661 gcgcctaaac tgtgaaaaag ttatcttagg ttgcacagaa ttgtcattga tgcatgaatt
    18721 tgctgaagat aaccattatc cagtgatcga tgcccaatct attttagcag atcggacgat
    18781 tgaacgagcg ctagctgaaa gaagcaaggc attggataca gcatcggaaa agtaaaaaaa
    18841 ttggatttgg ggcaaatatc ctaaaa
//
LOCUS       DASOUD010000051        18753 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.051, whole genome shotgun sequence.
ACCESSION   DASOUD010000051 DASOUD010000000
VERSION     DASOUD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.051"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            225..1271
                     /locus_tag="U4A29_001602"
     CDS             225..1271
                     /locus_tag="U4A29_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680728.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            1273..2532
                     /locus_tag="U4A29_001603"
     CDS             1273..2532
                     /locus_tag="U4A29_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEO0680729.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            2538..3119
                     /locus_tag="U4A29_001604"
     CDS             2538..3119
                     /locus_tag="U4A29_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEO0680730.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3201..4076)
                     /locus_tag="U4A29_001605"
     CDS             complement(3201..4076)
                     /locus_tag="U4A29_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO0680731.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINITLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            4224..4556
                     /locus_tag="U4A29_001606"
     CDS             4224..4556
                     /locus_tag="U4A29_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEO0680732.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            4668..5876
                     /locus_tag="U4A29_001607"
     CDS             4668..5876
                     /locus_tag="U4A29_001607"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO0680733.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            6030..6485
                     /locus_tag="U4A29_001608"
     CDS             6030..6485
                     /locus_tag="U4A29_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680734.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            6847..7122
                     /locus_tag="U4A29_001609"
     CDS             6847..7122
                     /locus_tag="U4A29_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680735.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            7135..9213
                     /locus_tag="U4A29_001610"
     CDS             7135..9213
                     /locus_tag="U4A29_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO0680736.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            9373..11259
                     /locus_tag="U4A29_001611"
     CDS             9373..11259
                     /locus_tag="U4A29_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO0680737.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            11271..12218
                     /locus_tag="U4A29_001612"
     CDS             11271..12218
                     /locus_tag="U4A29_001612"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO0680738.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            12238..12765
                     /locus_tag="U4A29_001613"
     CDS             12238..12765
                     /locus_tag="U4A29_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO0680739.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(12828..13481)
                     /locus_tag="U4A29_001614"
     CDS             complement(12828..13481)
                     /locus_tag="U4A29_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO0680740.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            13613..14455
                     /locus_tag="U4A29_001615"
     CDS             13613..14455
                     /locus_tag="U4A29_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO0680741.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            14613..15509
                     /locus_tag="U4A29_001616"
     CDS             14613..15509
                     /locus_tag="U4A29_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO0680742.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            15512..16225
                     /locus_tag="U4A29_001617"
     CDS             15512..16225
                     /locus_tag="U4A29_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO0680743.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            16245..16763
                     /gene="msrA"
                     /locus_tag="U4A29_001618"
     CDS             16245..16763
                     /gene="msrA"
                     /locus_tag="U4A29_001618"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO0680744.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            16760..16987
                     /locus_tag="U4A29_001619"
     CDS             16760..16987
                     /locus_tag="U4A29_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO0680745.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            17139..17690
                     /gene="lepB"
                     /locus_tag="U4A29_001620"
     CDS             17139..17690
                     /gene="lepB"
                     /locus_tag="U4A29_001620"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO0680746.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(17773..18273)
                     /locus_tag="U4A29_001621"
     CDS             complement(17773..18273)
                     /locus_tag="U4A29_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEO0680747.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(18552..>18753)
                     /locus_tag="U4A29_001622"
     CDS             complement(18552..>18753)
                     /locus_tag="U4A29_001622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEO0680748.1"
                     /translation="DNKKTSGALISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEE
                     EMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         6498 a         3193 c         3817 g         5245 t
ORIGIN      
        1 aaagcgcttg tggctctagg gctacaagtg cttttctttt tatttcctag cagaaaagga
       61 ataaataaga gacagagatt ccgaatccgt atgtcataca tagaaaacac atgtatttta
      121 tttaattttt atttatttgc aagttatgag gtgagctttg ctcttatctt ttctagaatt
      181 tgcttactaa tggtaaaata aaaataagaa gaggagggat gcgtatggaa cttgattatc
      241 aagtagaaga tgcgttgacg aaagtgtttt acggagaaac gctagtcagt cagctaagag
      301 aactttccat tgatcagcca atcttatttt taacgaatca gcgctattat gatttgtttg
      361 cagataagat caacccgtta tttgcaaatc aagcaaacaa tgactggtat atttgtgcca
      421 acactcaatg caatcattta acagaattaa agaacctgct tgatttcgtc aaacgctatc
      481 ccgaaaatca atccatgttt atcatcggat ttggaaatga agggatcatg gatctggcag
      541 gtttttttca aaaacataca acccttgatt catcactatg gatgatccct gtatcgatcc
      601 gttctctaag ccgagcatta acaccgcagc ggacgatttt gcagctacca aatcgtgtag
      661 tgctgcaaac catcaatctg ccagaacgca tcgtttatga tcaaacaatc agcgagcggc
      721 aggtggacgg aaagcagatt gactttttga cttttatctg ttgcggaatg atttgtgatc
      781 atcgtttttt gcaaaatctg tataaaaact atccaaataa aaagcaatta acgactcggc
      841 cgtttgccgg gatgatcgaa gaaatgatct atttttatca attagaggca gaaaacctgt
      901 caaattatgg taaactgttt gaacaagctt tttatctgac cgaaaatggc catctacttt
      961 ctgctagtat gaaaaaattt ttaggaatga tgtttcattt cgtgtggaat ctccaagcat
     1021 atccttttga tttccaacta aaaaactttt ttgtgtggct ggacttgctt ggctatccaa
     1081 tccactggct ggaacagatt tcaaagatgg agtatttgga aaaagttctt tttttggcag
     1141 aaaaaagcaa aaaaatactt gttttagaaa aaataggtat aatagacgga taccaacatc
     1201 caaacgaaaa agaattattg agaacaatga cggtatttca acaaatcgtc agtgagataa
     1261 gagggaattg aaatgaatac aaatagcgaa aaaatgttgc aagctttatc agatgaagac
     1321 ttgtcacaag cacaaatctt tttagaaaaa gcattaaaag aagacccagc agatatatta
     1381 gctgaactag gcgaagaatt gctggctatt ggctttttga tcgaagcaaa acagattttt
     1441 gaacaactga tcaaccaata cccagaaaat gacggactga acatcccgtt agcagaaatt
     1501 gcaatcgaag ataatgagat cgacttagct tttgaatacc tggaaaaagt ggataagcaa
     1561 agtgattact atccgcaaag cttattggcg atagctgatt tgtaccaagt tatcggaatc
     1621 cctgaagtga gtgaagcaaa attgaaagaa gcagctgggt tgcttcctga tgaaccattg
     1681 atccagtttg cgctagctga attgtatttt tcagttgatc gtttcaaaga ggcagcagtg
     1741 gtttatgaaa cattgttggc aagcgggata aatgaaattt caggaatctc gatgcaggaa
     1801 cgtttaggac aatccttaag tatgcaagga gaattcgaag cagctatcgt acctttagaa
     1861 gaagcactga aagaagaaag gacagatgat cgactgttcc agctagcttt tacgaatctt
     1921 caattgaaag aaaatcagca ggcaatcaac tatctggaag aacttcgaga agttaatccg
     1981 cattatcaat cgttatatct atatctagga caagctcttc aagaagaaga aatgattgaa
     2041 gaagcacaaa cagtgttaga agaaggaatc aaagaaaatc cttaccaagt cgaactttat
     2101 cacctggcct cggaaaatgc tttccgtctg catgatagac agcgagcaga agaactttta
     2161 ctgaaagctt tagaagtagg agacaaacaa gatgaaacat tacttacttt aagtaatctt
     2221 tacttggatg atgaaagata tgaagatgtg atacaagtga tcaatcagat ggaagaaact
     2281 gctaatcctt atgcagaatg gaacctagcc catgcttata atgaattaga agactttacc
     2341 gttgcagctg tacactatga acaggcttat catgaactat cacatgaacc agatttcttg
     2401 aaagagtatg cctttttctt aagagaagaa gggcagctga aaagagcgca agaattattg
     2461 acttattatt taacacttga accaggagat atggaggcac tgtcattatt agatgaccta
     2521 acagagaggt gagaaaaatg ctaattgatg tacgggaaaa gaagaatttt ttaacgtgga
     2581 tggtcaatca tgtatctttt agccgaagag aagttttttg gatcttgaat tatcttgcca
     2641 atcatgaagc gattttaagc aatgtccact ttgttgaagg ggcgaatttt gcggatcgag
     2701 gactgcagat cagtgatcta tctgttgaag gggaacccat gaaattgttt ttgcagggaa
     2761 aagaatttac ggacacagac cagatttttc atgaaatccg tttgaattgg aaaaagcctt
     2821 tatatgtgga atgtatattc gaaaatgctt ggcagacaag agaatatcta tctatcttag
     2881 aagacaatcc attttcttct tgggattctt cagtcagtga agaagatact gaattaattg
     2941 aacgatattt tcaagaaaaa gaagaagagg caaaactaaa aatgctttat gctcaaatcg
     3001 atcaagcact agaagatgga gatagggatg cttttttgaa attatctgat gaagttaacc
     3061 gaaagatcct tttgcagaca caaaagaaaa gtaaccgggt aagtgaacat aacaattagt
     3121 taaaaatagt atagtacaaa aaaagacaaa ggtcagctgg atgtggaatc tttcctcata
     3181 gcctatcttt gtcttttttc ttatagtttt ttcagtaatc ttgtctgtgg cttcaaataa
     3241 gtaaagcctt gcccttccac ttcatgcaca ttgatgattg aaaggaaagc tttttcgtca
     3301 atctcgtgga cgatcctctt agcttcagtt aattcactag agctaacgac catatagatg
     3361 attttttttg aattaccaga gaatgcgcct tctccagaaa ggaaagtcac acctctttgc
     3421 agaccggtca tcagtagtgg tgcaatctct tcgcttttgt tggagacgac aagtagacct
     3481 tttgcagaat atccgccatc taagatcgaa tcaatcacac gactgaaaac atacgaaaca
     3541 atcaacgtat acatcatacg tttcaagtcg atataggtta atgaagcaag taagacgatg
     3601 atatcaaatg ccaataggga acgccccata gtgatgccgt aattttgttc taaaatccga
     3661 gcgataatat cactgccgcc tgttgttcca cccatgcggt agacgatccc gcttcctaca
     3721 cctgcaacta aacccgcaag tagagaaacg atcagtagat catgctgtaa attgatttct
     3781 agtggaaccc tttgccagat ccataaaaag aatgacaaag aaagtgtacc tagaacagta
     3841 taggccaagg aacgtttacc gaggatcttt cctcctagta aaatcaatgt gatgttgatt
     3901 aataaagtcg tataagctgg atcaatacca aataaagctc ttaagatcaa ggtgatccct
     3961 gagactcccc cttctgctaa atgattagcg atgttcaagt aaacaagacc aaatgcataa
     4021 atacttgttc ctataatgat aaaaagaact tcttttactg tttttcgttt gaccatatga
     4081 attccagctt tcaattatgt attttcacag aatttccatt tagaaattcc ttcctaaatg
     4141 tagcatggaa attcgatttt ctcaattgct cttatctagg atttgcgtta aagttttggt
     4201 aagatagttt tggagggaaa aagatggata aaaagcgttc tttatacagt atgcagcagg
     4261 aagtggatga atatatccag caattcaagg tggggtattt ctctccatta gctcagatgg
     4321 ctcgattaac tgaagaagta ggtgaattgg caagagaagt caaccacact tatggagaaa
     4381 aaagcaaaaa ggcttctgaa ccagttaact cggtagcaga agaacttggt gatgttttat
     4441 tcgttacgat gatcatggct aattcattga atatcgactt aactgaagtt tttgaaaaaa
     4501 acatggaaaa attcaatcaa agagaccata atcgctttga gcgtaaggac gagtaaacaa
     4561 gatgaagtgg tcctggcctt gaagactctg aaacggactt gtcaaacagt caccatgcaa
     4621 ctgagtcttt atctcaggac aagaaacacg caggaaaggt tggaaaaatg aaattaaaac
     4681 aattacctct ggaataccaa aaggcaattc cagttctgaa aaaattagag aatgcaggat
     4741 atgaagctta ttttgtaggt ggaagtgttc gtgatatctt gctgaaccag ccaatccatg
     4801 atgtggatat cgcaacaagc gcatttccag aagaaatcaa acagcttttt ccaaaaacga
     4861 tagatattgg gatcgaacat ggaactgtgt tggtattaga aaatgatgaa caatatgaga
     4921 tcaccacatt tcggacggaa tcaacttatc aggactaccg tagaccagat catgtcgaat
     4981 tcgttcgttc gttggcagaa gatttgaaaa gaagagattt tacgatcaat gcgtttgctt
     5041 tgaaagaaga tggagaaatc atcgatttgt tcgatggtct gcaagatcta gaagaacaga
     5101 tcttgcgagc tgtaggcaat cctcatgagc ggttccatga agatgcctta cgaatgatgc
     5161 gcgggttgcg ttttgtcagt caactagggt tcaaattaga ggaagaaacc ttctacgcca
     5221 tcaaagaaaa ccacgagcta ttaggtaaaa tatctgtaga acgtataaat gtggaattca
     5281 tcaagatgct cttgggaaat gaacgggccg cgggaatcca tgcatttatt gaaacagagt
     5341 gctacatttg ctgtcccggt ttgaaagaag aaggcgaagc acttttacgt tttgctgaat
     5401 taccatccgt cccattgaaa gaagagagcc aagcatgggc attattgatc agccaactgg
     5461 atctaaaaga aaatgagatt cgttcctttt tgaaatcgtg gaagtgttct aaccaaatga
     5521 ttaaagacgt tcaagcactt gtccaaggac tacgacaacg agaaactgga gctttggaac
     5581 cattcgaatt gtacttctta ggcaaaaaac atgcactgca agtagaagaa ttgatgatct
     5641 attttgacga aacacctcag atggatgccg taggagaatc ctatgatcgt ttacctatca
     5701 agtcattaag cgaattagca gtagatggta aaatcttgct agaagaaact ggaaaaaaac
     5761 ctggtaagtg ggttagtgaa gctttgcaat tagctgaaaa agccgtcgta gaacaaagaa
     5821 taaaaaatga taaaaaacag gttttggatt ttttatcgaa agaaaaattg atttaaaaaa
     5881 cactagactg tatctgctca gctttgttgg ctacttaaag tgggcagata cgtcattaga
     5941 agaaaaaaca taagatgtga ccacttacat aaatttgtgc taaactgagg tagtacaaga
     6001 ataaattaca tcataaagga gtctttttaa tgagtaaaga aatgacagcg ctaaaatttt
     6061 attttagaaa tggagaaacc tggacgatca accgccgtca tatcggcgat ctttggatca
     6121 aacaaatcac aacaagtttt ggacgaatca atggcagtga atttgttgaa atccatccgt
     6181 gtgctggatt taaaatcgaa atcttccatg aaggggacgc agttgcgaca catgatatca
     6241 acctaggcgg actggaaatg ggcatgttta atcgcgcatt gaaatacgaa gatatcgagc
     6301 gaatggaaat cctttaccga aatggcacac ctgatcttgt gtacttccca tatctagata
     6361 aaggaacaga aggattagac aatcaatatc aatcaacaaa aatcagcgaa aaaacaggta
     6421 acttgtatat cgtgatcaat ccagatcaac gtgtagaaga tgtatacggg gaatttttcg
     6481 aataaatcga tttttacgca aaaatgaggc tgtgaaagag atgtgtctta ttactactga
     6541 taggctgtct cttccacacc gtttattcag aataagggac tgtgacaaga tttttgtcgc
     6601 agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa agtaaacatc
     6661 cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac tatttggaat
     6721 agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga tgaaacaatg
     6781 aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt ataggagatc
     6841 tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg ctatggcgcc
     6901 ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa tatattgatg
     6961 tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa acgtgtacag
     7021 tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat tttaaagaaa
     7081 aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg gacgatggaa
     7141 tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat agctagccgc
     7201 attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt attaggtctg
     7261 acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat gattattgca
     7321 ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa aaatttcggg
     7381 acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt aggtgcgacg
     7441 ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg agcagcgtta
     7501 ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat ccctaaaaag
     7561 gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt tactgccttc
     7621 cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc ggggatgagt
     7681 ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg gttcaaacaa
     7741 aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta tctgctgatc
     7801 gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt atctggaatc
     7861 attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat ctctgtgttt
     7921 gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt tacactgaat
     7981 gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc agtatgggga
     8041 gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc tgtcatgctt
     8101 tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg cagtaaaaag
     8161 ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa gggaacagtc
     8221 agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt ttaccagcgg
     8281 tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat agggatcatt
     8341 gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa tgctttgatg
     8401 attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga tcgtaataca
     8461 aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat acgtgatctg
     8521 tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca agcactgatt
     8581 ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct ttcttctaat
     8641 ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac aagccagatt
     8701 ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat cgttctccat
     8761 ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag tgatgaagat
     8821 atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga aagtttagat
     8881 aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca acgcaaactt
     8941 gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca gcaagatagt
     9001 ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat tgatgaatac
     9061 gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa tatcaatttg
     9121 ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt tgcgtttcga
     9181 gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca agttttataa
     9241 aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga gaacacaaaa
     9301 agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca atatccaaga
     9361 taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac ttatggtgaa
     9421 aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat cgggttgatt
     9481 gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat cgatagcagc
     9541 gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta tctttctcaa
     9601 gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg aaacagcccc
     9661 ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga aaacggagaa
     9721 gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa agaagatgct
     9781 tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag tgaattagat
     9841 aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc gcaagtgttg
     9901 attgatgaac ccgatttact attactagac gaaccaacaa accatttaga ttatgaagcg
     9961 atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat ggttacccac
    10021 gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt tggaaaatta
    10081 tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg tgatcgtgta
    10141 gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc ttggatgagg
    10201 gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt cgaagatcta
    10261 aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct agcgacacaa
    10321 cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa tgaccaaaca
    10381 ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat tactggtaaa
    10441 aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc cctggatagc
    10501 ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga aaatgaagaa
    10561 atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga agcaaaaaca
    10621 gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt tccaagattt
    10681 atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt atttttgttg
    10741 aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa tgatttggac
    10801 atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc tgtgatcact
    10861 gtttcccatg accgttattt cttggacaag gtagcagata agttattagt attccaagga
    10921 aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa gcaaaaagag
    10981 acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc tgctgaaaag
    11041 aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga gattgcagaa
    11101 cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga taatttcact
    11161 cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga agaaaaaatg
    11221 gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg atggaacaag
    11281 cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa gacagaacag
    11341 gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa aaagggtttc
    11401 ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg ctttggtttt
    11461 taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt tgggatgaat
    11521 gggcatttga acgctacatc aaaagtccag actataatgg accagatatg actgattttg
    11581 gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag agcaagcttt
    11641 tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat cttggacaca
    11701 tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc gatcaaatca
    11761 aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc gtttctgcat
    11821 ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg tttcaatttt
    11881 atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat gtgtttctag
    11941 gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca catgaaacag
    12001 gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat atcaatcata
    12061 tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact ttagttttga
    12121 atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta gaaggatatg
    12181 acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga tgagaaaatg
    12241 ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga ccgtcttcct
    12301 tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa tacactagtg
    12361 atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg tcagacgatt
    12421 gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca tagtgtggaa
    12481 gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg agggtcagct
    12541 gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat ccatcatgta
    12601 ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact gatcaatact
    12661 agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac gtatcagcgt
    12721 aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag ctatggatag
    12781 actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta agtaaagaat
    12841 aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca aacaacatgc
    12901 atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga aagcccgcct
    12961 gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac agcagccacg
    13021 acacagagcc accccataaa aatatagatg atcgtagata cttttgatac tctctcccgt
    13081 ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca gattaataca
    13141 aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt ataacttcct
    13201 gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt tgcttttgta
    13261 aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat cgatccatag
    13321 atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac taaaagaacc
    13381 actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt aaacacttca
    13441 tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc tcctttacta
    13501 ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa ttgtcttaca
    13561 tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa taatgacaaa
    13621 tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg atgctttagg
    13681 cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg atgatgacca
    13741 actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc ccaaaacaag
    13801 ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag acggcagtga
    13861 agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag caagacaagc
    13921 cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg accaaggatt
    13981 atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag tagagaaaat
    14041 ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa tatccacact
    14101 agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt ctagtgtttt
    14161 gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa ctaaggggcg
    14221 aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga aagaattgcc
    14281 aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc aagaattcaa
    14341 agagggtttg caagaattat tcccggacat gcatatccct gtactgcata cgaatccggt
    14401 tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag attagtcgaa
    14461 aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct ttataaaatt
    14521 aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat ttttgataac
    14581 attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga catttgatcc
    14641 tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct gcttctatac
    14701 cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca aataaaaaag
    14761 aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga gatcaaacca
    14821 agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat aatttgaccg
    14881 cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc ttaaagcgag
    14941 taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc acactgactg
    15001 ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta acggtcaatt
    15061 cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg gaagaatttc
    15121 gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca ttttatttga
    15181 actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa ggaacagaaa
    15241 cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg ctttatcaag
    15301 gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact agcacgacag
    15361 aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc catccaaata
    15421 atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag acacaaaacc
    15481 aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg tatcttcaaa
    15541 caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg gggtagtact
    15601 tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag aactcccaga
    15661 aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt caaataaaga
    15721 aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata gtgatattga
    15781 atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag tactaggttt
    15841 tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa atgtccaact
    15901 aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg tgatgaatat
    15961 gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg atctgactat
    16021 catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag cagataaaat
    16081 cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata cctctgatgc
    16141 ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca agacaaaaga
    16201 aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa cgagcaatat
    16261 ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct ggtatccata
    16321 cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa caagtaaaaa
    16381 gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca ttgatttcat
    16441 atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct ttcggacaat
    16501 tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa gaacagcgga
    16561 tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa cagccaatcg
    16621 ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat caagagtatt
    16681 ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc tttatcgaag
    16741 aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga cactgcgagg
    16801 cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag acatccaatt
    16861 tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga atgtcgacta
    16921 tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa ataatcatga
    16981 catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa tagcctaatt
    17041 gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt tcttataata
    17101 aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa caacgctata
    17161 ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt gcgttcatgt
    17221 tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa aacgtgttga
    17281 agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt gatattgttg
    17341 tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt ccaggagaaa
    17401 atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag gaaccttatc
    17461 taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac tttacactac
    17521 aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt ggagataatc
    17581 gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat attctaggta
    17641 aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga gaaacaataa
    17701 agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag tttactacct
    17761 tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag ttttgataaa
    17821 tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga atctacctag
    17881 aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat tgataaaaag
    17941 gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt ttatcttttt
    18001 atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa gaaaagcagc
    18061 ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca tttcaagcaa
    18121 accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa atccgaatgt
    18181 gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa gggctaataa
    18241 aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa cgtcttttgc
    18301 aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga taccataaaa
    18361 tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac aattaaaaaa
    18421 agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata aaaaaaatag
    18481 aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa aacgtcagat
    18541 tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc tagatcttgg
    18601 acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg gaaagtacca
    18661 ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc aacactaaac
    18721 gaaatcaaag caccacttgt ttttttatta tca
//
LOCUS       DASOUD010000052        18582 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.052, whole genome shotgun sequence.
ACCESSION   DASOUD010000052 DASOUD010000000
VERSION     DASOUD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.052"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            154..237
                     /locus_tag="U4A29_001623"
     CDS             154..237
                     /locus_tag="U4A29_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEO0680749.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            362..1099
                     /gene="erm(B)"
                     /locus_tag="U4A29_001624"
     CDS             362..1099
                     /gene="erm(B)"
                     /locus_tag="U4A29_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEO0680750.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1362..2225)
                     /locus_tag="U4A29_001625"
     CDS             complement(1362..2225)
                     /locus_tag="U4A29_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEO0680751.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2227..2499)
                     /locus_tag="U4A29_001626"
     CDS             complement(2227..2499)
                     /locus_tag="U4A29_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEO0680752.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2516..2725)
                     /locus_tag="U4A29_001627"
     CDS             complement(2516..2725)
                     /locus_tag="U4A29_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEO0680753.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2824..3720)
                     /locus_tag="U4A29_001628"
     CDS             complement(2824..3720)
                     /locus_tag="U4A29_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEO0680754.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            complement(4018..4590)
                     /locus_tag="U4A29_001629"
     CDS             complement(4018..4590)
                     /locus_tag="U4A29_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEO0680755.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            complement(4866..5660)
                     /locus_tag="U4A29_001630"
                     /note="aph(3')-IIIa"
     CDS             complement(4866..5660)
                     /locus_tag="U4A29_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEO0680756.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5753..6295)
                     /gene="sat4"
                     /locus_tag="U4A29_001631"
     CDS             complement(5753..6295)
                     /gene="sat4"
                     /locus_tag="U4A29_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEO0680757.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            complement(6292..7200)
                     /locus_tag="U4A29_001632"
     CDS             complement(6292..7200)
                     /locus_tag="U4A29_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEO0680758.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            complement(7233..7967)
                     /locus_tag="U4A29_001633"
     CDS             complement(7233..7967)
                     /locus_tag="U4A29_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO0680759.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(7948..8817)
                     /locus_tag="U4A29_001634"
     CDS             complement(7948..8817)
                     /locus_tag="U4A29_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEO0680760.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(8832..9056)
                     /locus_tag="U4A29_001635"
     CDS             complement(8832..9056)
                     /locus_tag="U4A29_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0680761.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            complement(9199..10743)
                     /locus_tag="U4A29_001636"
     CDS             complement(9199..10743)
                     /locus_tag="U4A29_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0680762.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            complement(10763..11179)
                     /locus_tag="U4A29_001637"
     CDS             complement(10763..11179)
                     /locus_tag="U4A29_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO0680763.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            complement(11180..11677)
                     /locus_tag="U4A29_001638"
     CDS             complement(11180..11677)
                     /locus_tag="U4A29_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEO0680764.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            complement(11988..12665)
                     /locus_tag="U4A29_001639"
                     /pseudo
     CDS             complement(11988..12665)
                     /locus_tag="U4A29_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(12665..13282)
                     /locus_tag="U4A29_001640"
     CDS             complement(12665..13282)
                     /locus_tag="U4A29_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0680765.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(13296..13466)
                     /locus_tag="U4A29_001641"
     CDS             complement(13296..13466)
                     /locus_tag="U4A29_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680766.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(13814..15304)
                     /locus_tag="U4A29_001642"
     CDS             complement(13814..15304)
                     /locus_tag="U4A29_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEO0680767.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(15347..15646)
                     /locus_tag="U4A29_001643"
     CDS             complement(15347..15646)
                     /locus_tag="U4A29_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680768.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(16012..16296)
                     /locus_tag="U4A29_001644"
     CDS             complement(16012..16296)
                     /locus_tag="U4A29_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0680769.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(16815..17300)
                     /gene="vanZ"
                     /locus_tag="U4A29_001645"
     CDS             complement(16815..17300)
                     /gene="vanZ"
                     /locus_tag="U4A29_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEO0680770.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(17453..18364)
                     /locus_tag="U4A29_001646"
     CDS             complement(17453..18364)
                     /locus_tag="U4A29_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEO0680771.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         5332 a         3746 c         2839 g         6665 t
ORIGIN      
        1 gattcatcgt cattcctcct gctatctttt tctattattc taccttattt gatagtagat
       61 ttaaaacttt gcaacagaac caaaattttg tataatagga attgaagtta aattagatgc
      121 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgtaatgta
      181 gataaaacat ctaccgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      241 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      301 acaaacaaat cgtttaactt ctgtatttat ttatagatgt aatcacttca ggagtgatta
      361 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      421 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      481 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      541 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aacactcgtg tcactttaat
      601 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      661 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc
      721 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
      781 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
      841 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
      901 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
      961 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1021 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1081 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1141 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1201 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1261 aaattcctcg taggcgctcg ggaccccaac atgaggaaat gtttttataa aaaaagagta
     1321 gttaccgttt tttggtaact actcttttcc ttttttaagg tttaaatacc tggaagttta
     1381 ggtgttttgg gtattggtgg tgtaggtagc tgcaagcttt ccattttttg ttgaattgct
     1441 ttaaattcag gggtttcttg gtgttgattt tgaaccattt tttgctctat tctctccaaa
     1501 gtcggttgaa tttctttccc tgatacttta cgatttaatt ctctttctaa ggtctctttc
     1561 ggactaatgg aaggcgtttc taagcttgaa tagagtttca ctccttctct attgtaaagc
     1621 cttatatcgc taaataagcc cgttttatga agggtttcta aattggtcgg taagtttttg
     1681 acaacaatat catgcgcttg ttttggtgtt gccctagctg tcattggatc atctgcatac
     1741 atggtttcat atcgttcaat cgttcctaag tatgattcaa ttttcggtac tgccatgacg
     1801 tatatttttg tttcataacc tttagattga agcattgtgg ctgtttgaat gggaacgtct
     1861 gttgttcgtc ctgttccttc aatcactaaa ttatagcctt gatcgcttaa acggctaata
     1921 agtgcttctg tcatgcgatt cgaataaggg gtaacgtgtt ttactacgtc tttttcatag
     1981 agtttcacta attcgtcaaa atttgggtgc tgttgtttaa aggtatcatt atcaatgaca
     2041 ataacattcc cttgtgtttc ttcaaaaatt gctgatcgca aactggtttt ccctgaccct
     2101 ggttgcccac caagtaaaaa agcggttggc gattcaaccg ctagtctatt tttagttagt
     2161 cgttcaacat tcttttctaa acgatcttca aattgctttt cggtgaagtc agtaatattc
     2221 gccatgttaa gccactttct ctttattcaa aatttgtttt gaatattgat tcattgaccg
     2281 ccaatactca tgaatagctt gtaattcttc aaatggttgt tgaaataaat caacttcttg
     2341 tgccacttct aattgattta aaagattcac ttctagtaaa cgagtatttt tagtatctag
     2401 ttcatgattc aaaacataat ttaatacccg attataaaca cttgccgata actcattaat
     2461 gatttcaatt tcaaatgttt tttcatacgt aactgccatt tcattttcac tccttttaaa
     2521 gtttgtcagg taaatatttc cgtatatatt cttctaaggc ttgatccata acttctttta
     2581 cgtttccgcc attctttgct gtttcgattt ttatgatatg gtgcaagtcg gcacgaacac
     2641 gaaccgtctt atcccccatt atatcttttt ttgcgctgat tggcgtatca tttcgttttg
     2701 ctttctgtgc gcctaaattt cccacgatcg ctcacttctt tctatttctt cttactattt
     2761 attttatcat cacctatcac aaatcacaag tgatttgtga ttaatcactt gtgatttgtg
     2821 attctattct ttttcatttt ctaattgaat gattcgctca atcatttcaa aaaatacgtt
     2881 ctcatacata gacaaaactt ttttgtcata gcctttgtgt tctgtaatgc catttttaga
     2941 ccaagtactt actttattac ttcgcttgat aatatttcgg aaaaccaaat tatcctcttt
     3001 atgttctttg tacagttctt ccaggtttga ttttatcgtt gcgctgtccg tatcaactaa
     3061 ataaggaaca aaaccaatca tatctagtcc agggttaaac tgttcttgca aatcaatcaa
     3121 ataggaaata tagttttgaa tgttgtttgt actttcttct tctgcttgta aagggatcat
     3181 aacgtaatca ctcgccacga ttgcattatt tgtataaacg cttggcgttg gtacagtatc
     3241 aataataata agatcatagt cactttttaa aggtgctaaa agagtagcaa gcaatctact
     3301 ttcattttca aacgtccatg agcgagttaa ttttggcagt aacatcaaat caaacgtgcc
     3361 agggatcaag tctaaattat cagtcaaatg aataatagaa gaagccaagt ttccattttt
     3421 caagccttca taaaaattga cacgtggcaa ttctacctca aaagtttttg ctaagtcttt
     3481 tgtcaatgtt gcctgtaagt ccttatcgat cattaaaact tttaaattca atttgtctgt
     3541 caagtaagca aacatagtcg ataatttgga ctttccaaca ccacctttaa agtaattatt
     3601 caagataata attgcttcat ttttattgtt taaaatacgt cttaattctt caagtatttt
     3661 tagtttttcc ttctccataa ctacgcccca ttcttttttt gtatagtaat attgtatcat
     3721 taagaatcac aaatcacaag tgatttgtga ttaatcactt gtgatttgtg ataagtgatt
     3781 tgtgattaat atataaaagc cctctttaaa gggcttttat atttattttg agaaagatat
     3841 aaaatcaata tatccctttt ccccaatttt tacaacggca ttgtagggca tatagttttt
     3901 tatatgaaag catttgtttt aatatttgtt ccaaagagcc atcatggctt tgacagagct
     3961 cttcttctat gcaaatgctt ttaatccgat ccacacgaaa attcctatat tcatttctta
     4021 gcctgcaaaa ccgattaagt accagttctg ttcataaaac cccagtgata taggctctat
     4081 cattctgctt tccggttctt gccctcctga tgcaggatat tgaattgata ttaccctttt
     4141 attgcagatt gcagtctgaa ttgctgcaat aacgttatcc gccaatgtat ttttctgtcc
     4201 agatgtgctg tacacgttaa tactgttctc caactcctgt gcatagtttt tttcaccatg
     4261 cttcaaaata gatttgattt tatacatagc tgaatcaaaa tcctttataa atgattcatc
     4321 tccatggcaa tttaaaaatt tccctgctgt aatcaatgca cccacttccg ccgctgtaaa
     4381 cattaccggc ggaagtagga agccctctac aagaaaatat ccttttccgg cctcagatcc
     4441 aattggtatt ccggcttctt caagtgtccg gatatctctg taaactgtcc ttaagcttat
     4501 attaaatcgc tgtgcaattt ctttggcagg aattattttt tttgattgca gctgaataag
     4561 aatagaggtt actctgttaa tcctgttcaa attttattcc tcctctgtga cttccataaa
     4621 aagtatatcc tatgcgttct taagctagct gctgttagtg cttctcattg caccctggtt
     4681 ataaaagcca tactcacaaa cctcttcacc aaaactcttt acatctctaa tatagaactc
     4741 tatctagcga acttttagaa aagatataaa acatcagagt atggacagtt gcggatgtac
     4801 ttcagaaaag attagatgtc taaaaagctt gtagttaaag ctttttagac atctaaatct
     4861 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     4921 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     4981 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     5041 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     5101 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     5161 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     5221 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     5281 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     5341 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     5401 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     5461 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     5521 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     5581 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     5641 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     5701 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     5761 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     5821 cgacggagcc gattttgaaa ccacaattat gatagaattt acaagctata aggttattgt
     5881 cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat
     5941 tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta
     6001 tataagcgta ccggttccaa ttttttcgca gtttaacttt tccgacgcat ttatcgtctt
     6061 ggtagtaaag atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct
     6121 catcctcttc atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt
     6181 ctgtaaaggt ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg
     6241 gttcgctggg tttatcgata tctttcaggt gccctgcttt catttctgta atcactgttc
     6301 ccgcctctct tctatatcag cggcatatgt gctatccagg cagccggttt taccagtgta
     6361 ttttttatac atgtccctgg tatattttgt tatattccta tcatactccg gataggcata
     6421 atgaagcctt tccgccacct caccggatac cgccctgaac aattgatggc atagaaataa
     6481 tgcttcccat atgttttcat aggaatccat ccggtaggtg gacaaaagtt tctcccacaa
     6541 atcctcggat atataccttt caataaactt atagttcttg cctacactta atttaaagcc
     6601 tgtttcgatg ccgaccttcc atgatatcat tctcagcagc tcatggcgaa caatctgatt
     6661 aaaatgatca atagcaaata aaatttcctt acggcacaat cctttaataa cataaggtgt
     6721 tacattccaa aattcattgc agcaatcatc atactccctt gcgcttggct ttcttacatg
     6781 ataatctata tcagtcggaa ctatgtccct tttaattcta caatctttat caattagaac
     6841 ctttattaat ttatcgccct ttaggtaatt atctaactct tccaagggca ataaggtaag
     6901 atcaatttta ttgtaatcat caaatagcat aagataggaa aatccctttt cttcaggtgg
     6961 gaataattcc atatcctccg gcttttgcat cattattata ttcccaaatt gattaagcca
     7021 gtcatcatta gatataaacg gttctatatc acttacaaaa tatgtaatat cataatcctg
     7081 aaattcatct ttaggtatat taatatttgc gcgtgacccc tcaagggtca caattcgaat
     7141 acgttcatcc tgttctgcta aagaaagtac taaatccatc atttcttttt ctgatctcat
     7201 ttacatactc ctcgtttatt ttttctatat tattacatct tcttttttgc cgatacaatc
     7261 agcatcattg ggcgtcgcat ttcatccgcc atccccggaa tatccatcat gttctctggc
     7321 ggctgtggct ccacaatctg atttattata aaactatttg aaagcagtgt atttagatat
     7381 gtggtcagtg ttctatgata ttttgtaacc ttttcttcca aaaacatagc tgtccgtttg
     7441 ccctcataat aataattgtc caccgggaaa tgcagtattt ctcctttttc gttataatac
     7501 cagtcttgtg ttccatgagc agtaaaaaca ggatgttcaa ctgtaaaaac taaattgcca
     7561 ccagccttca gcatcctata tatctttttt attaaattct catagtctgc tacataatga
     7621 aacgcaagcg aacttagtat tacatcaaag ctctcctctg ggaaatccac atcttctatg
     7681 gcacagcatt catattcaat ctgtggaaaa tgggtttttc cttttgctac ttcgagcatt
     7741 ttatgagaaa tatcaacacc tactacagag gaagcaccgt tttccatcgc atatatacag
     7801 tgccatccat agccgcatcc taaatcaagc acacgcttac ccttaaaatc aggtagcatc
     7861 tttttcaaag tctcccattc tcccgcacca gccagtcctt tctgcgagcg actcatttga
     7921 ctgtattttt gaaaaaatat attatcatca tatttgtttt ctttcatctg aactctcctc
     7981 gtttaaaaag ttatttatct ccgatacaat ttcattcact tctttataaa gtttctcggt
     8041 catgtcgtag cattcaaaaa gagaaagacc gagtacttca aaaatatgat taactctttc
     8101 ggcatatttt tcaggtttat attcaaaagt ttcaagcagt tttatagctt tcttttcgtt
     8161 aatgcaataa gcattattac atgcaaatag tacttgattt aagcatgaaa taatacgaaa
     8221 aacatggcct gcaatataat atttatcctc tgcccccgca tttgctttta caaacattaa
     8281 agagaattca gcttcaaata taaaaaagtt catcaagctt ttctttagag cagtagggta
     8341 aatttctgcc tgctttttta attcgcataa gctttcgttc ttagcatata gtattttact
     8401 aatcgctaat tctccacgat acatagcact tatataacca tggggatgcc cggtctgata
     8461 attggcagta acaattcctt gctccgtatc tttgattatt tgttccaccc gttttatatc
     8521 acgtaatatc aagtcaacat gacacccgtt tataactaac catccaccgc cattaaccca
     8581 atcaccccac gctccgggag gtacaacaag gttgtttcta ttctcatcat ccaactctgt
     8641 agcaatttga ttaatcgctg tcaggtcaaa tgagtctgaa ttgtaataga ttccgatatc
     8701 tatatcagaa ttttctgtat gggtgcctct tgcacgtgag ccccctaata cgatgccttc
     8761 tataaaaggc aaagatgata atttctctgt tactatttga ataacattac ctaccatata
     8821 aacatcctcc tttattcgtt agatattttt atttcttctc catctggtaa aataaatgcc
     8881 tgcacaaact tgacacctag cacatcagca atggccttca actcatccaa ggttactgtt
     8941 tctcgttgta attttttatt gaaattctgt ggagtctggc caatacgtct agcaagttcg
     9001 gaaacactta tattcatttg ctcacacagt tctttaatca tttctgccgt agtcatacag
     9061 cacctccaag tttacgctaa gaatattata aaccatttgg ttgataaaca caatagttat
     9121 taaaatatta ttataatcag caagcaaaag aaaacccaca actctactgt agagtcatgg
     9181 gctatttact tctggttttc atatttccat ttcgatgcca gatttgaagg ctatgacgaa
     9241 gtggccttca tagactgtaa cgctctggat tatcttcctt acaagcttat catcatattc
     9301 taaggtgcgg aatttgttct tgcggataaa ttcaatcagc tcattgattc gctcgttctc
     9361 gccactgagg gaggcatctt ctactaaaag gatctgtcgc ttgtctctca gctcatcaat
     9421 ctcatctgct aagtggtcat agtcttgacc cttgttcgcc agcttgatta gttctttttg
     9481 tttttcctct agtgagttgt taatctctga aatttggtat tctgtggttt cgccaatcac
     9541 agcatgaata ttctcttcca gtgtctttat catattgttc ccacctgcaa gtagcttatt
     9601 aatcgcagtc attactgcac cataaagttc atcttctttt acggttcggt tcttacaaac
     9661 ttcaggacct tgctcgattc tagtcacgca tcgccagaca aattcttttc ttccgtggat
     9721 attccaatag acccgtctat aaatatcgcc acaatctcca cagaaggtga tggcacttaa
     9781 agcgtacttg ctactgtaaa ttcttttgtt cttgccttct cctgtgtaga tattgcttct
     9841 ccgatgaatt tcttcttgcg cctgcaaaaa taactcttta ggaataatcg cttcatggct
     9901 attttcaaca taatattggg gaacatgccc ttcatttttc actcgtttct tggtcagaaa
     9961 atctactgtg acagtctttt gcagaagggc atctccgatg tatttttcgt tttgaagtat
    10021 cttcttaact gattctggtc gccatcttgg ttttcccgct gctgttaaaa taccatcctt
    10081 ttcaagggct cgaccaatgc ccactagact ctgaccctca aggtattctc ggtagatgcg
    10141 tttgataatc tcagcttctt cggggacaat agttaaattt ccatcttcat ctttcgtgta
    10201 gcccataaat cgcttatggt tgacctgcac ctttccttgt tggtatcggt actgtagccc
    10261 aagcttaacg ttttgtgaaa ggctctggct ttcctgctgt gccaaagatg ccataatagt
    10321 cagcaaaacc tcacctttgg catccgtggt gttgatgttc tctttctcaa aataaactga
    10381 tatgttctta tccttgagct gtctaatgta ttgaaggcag tctagggtgt tacgcgcaaa
    10441 tcggctgatg gattttgtaa taaccatgtc gatgttaccg tccatacact ctgcaatcat
    10501 gcgattaaat tcgtcacgct ttttagtgtt cgtaccagag ataccatcat ctgcaaagat
    10561 gccagcaaac tcccattcat tatttttctt tataaactct gtatagtgtg agacctgaac
    10621 ctcatagcta gaattttgtt cttctgtttc tgtagaaaca cggcaatagg cagcaacacg
    10681 aagtttcttt atcttttctt ttgcggctgt acttcctact cttttacgag caggaataac
    10741 cattatattt ttctctgtca ctttattcct cgctttctat cagactgtat aagtattctg
    10801 ctcgttcaaa aggatccact ggcatcttat tatctgcctt tttcattttg aatcgttctt
    10861 tgggaggggg agaggtgaaa gcggcaagct ctaccactcg tcctaaatcc tttgcacgtt
    10921 tatctctaac ttcttcagct ttatcaaata tctctttatc aataattgct ggatacgtat
    10981 catttccaag gtagttgacg tttttcaaaa tgcgccccat cacagaatgt gtcttttcaa
    11041 tacctgcctg ttcgccagcc actgtaaggg atagtcctga tatgtatttc tcaaagaata
    11101 cctttacttg acctgctgcc ttttcatcga cagtaaccac tccatcttga attttgtatc
    11161 catatggaat atatgccatt tatctcacca ccttttcttt cagggaaaga ccgcatttca
    11221 aattgaatgt cagctcatcc ctggaattta caatgatgtt cactacaaat tcttcaaata
    11281 gttcttccgt gtagtcacca ttaaaattat ctgctgacac ataattaatg aggtccttta
    11341 tctcgtttgc tcgcaaaacc ccactcgcag agttcgttac caggtttgtt ttttcggtag
    11401 taagattttt aatctcacta tctaaaacat ttcgttcctg attaaaaaga gcaggttcta
    11461 ggaaaccttt ggacatcagt gcaataaggg tattgcgttc ttccaatagt tgctccatta
    11521 gcttatcaat agcgtccatt ctttcacagt cgctttcttc atcgatttgg ctaactgact
    11581 tgaaaagtgg ttctaggatt agcttatgac tgaaagcaag cttattcatc atggttgtca
    11641 atgtggcttt gatttcccca tctcgcaaaa acaacatgga gcaactttct ttatcttcga
    11701 tactatctca acatgtgtcg tggataaggt aagtatgtaa aatggtatgt ggaacatagt
    11761 gatttgctgt aaatgatttg ctaactactt tagttcttta tacaaagtat aaatcggata
    11821 ttttattatt ttttctttta tatcttctga gattaggtca aagtatttgt ccgctaaatc
    11881 agaaaggggt tcttttttca tcacctaatg ggtgatgaag cagaatactt taaagactat
    11941 taaatcaatg ttaggaaaga ctattagtaa aactatgaat tattcaggaa aaaaggttct
    12001 tttttaaagt tttctattgc ttcctggcgt tgataaatga gatataaagt cggtgtttgt
    12061 acacgtccta atgaaaatgt accttgtacg cctttttgtt gtaaattcaa ggtatataaa
    12121 gggcttgcgt tcatgccgat caaccaatcg gcaatttggc gtgtttgcgc ttcttgataa
    12181 aagggatagt aattcattcc tggttgcaaa ttttgaaaac cgcttcggat cacatctttt
    12241 tctaagctat tgatccataa tcttttatac gttttatctt tagaaaaggc atttgcttta
    12301 tggataatcg accaggcgat attttcacct tctctgtcgc tatctgttgc gacaataatt
    12361 gtatttgcct ttttgagaag ttcagcaaca attttaaact gctttccctt atcttttgca
    12421 acttcaaaat cgtatcgatc aggaaaaatc ggcaaagatt caagtttcca attttgccac
    12481 ttttcgtcat aatgacctgg ttctgctaat tccactaaat gcccaaaacc aaaggtgata
    12541 aacgtttcat ctgtaaatag tgggtctttg atctcaaaat aaccgtcttt tttggtgctt
    12601 tgttttaaag cacttgcgta ggctaatgcc tggcttggtt tttcagctaa aataaccgta
    12661 ctcattaact atccctcttt tcattgtttt ttctttgatc gactgtcacg ttatatcttg
    12721 ctcgatacct tctaaacgtt cggcgattga ttccagtttg ttcttcaact tctttatcgg
    12781 ataaaccatt caaaaacaaa tcgaaagcat gctttaaacg tggatcattt tctttaaatt
    12841 tatgctgacg acctttgaat ttgccttttt tcttagcaat ttcgattcct tgtgcctgac
    12901 gttccttaat tttttttcgt tctgattctg cttgatactt gtacaattca atgacaaggc
    12961 tattaatcaa acgccttaaa ttttcatctt caataccatt cattgagggt aaatttaaga
    13021 cttccagggt tgccccctta atttgaattt gattcatcaa ttctgttaat tctttattat
    13081 ttcgtcctaa tcgatctaat tcagtaacaa taacaatatc cccttcacga atatagttaa
    13141 gcatagcttg taattgtggg cgttcgaccg attgaccgct taatttgtct gaaaagacct
    13201 tagaaacgcc ctgtaacgct tgtaattgcc gatctaagtt ctgttctttg ctactgacac
    13261 gtgcataacc aattttagcc attttcaacc aacctctaaa attctctcgg ttgcaataac
    13321 caatcagcaa tatctacttt ttcaatttca aattgcttat cagaaattgt cttttcgtaa
    13381 gcgataaaat cttgcgcata ttgttgctca ttaaaaatag ccaccacttc gtcattttct
    13441 aaaactcgat aaataaattt tttcatttta ctcctcctat tatgcccaac ttaaatgacc
    13501 tattcaccaa gtcaattata ctgctaaaat catattagga caaataggta tactctaatg
    13561 acctataaaa gatagaaaat taaaaaatca agtgttcgct tcgctctcac tgcccctcga
    13621 cgttttagta gcctttccct cacttcgttc agtccaagcc aactaaaagt tttcgggcta
    13681 ctctctcctt ctccccctaa taattaatta aaatcttact ctgtatattt ctgctaatca
    13741 ttcgctaaac agcaaagaaa aaacaaacac gtatcataga tataaatgta atggcatagt
    13801 gcgggtttta ttttcagcct gtatcatagc taaacaaatc gagttgtgtg tccgttttag
    13861 ggcgttctgc tagcttgttt aaagtctctt gaatgaatgt atgctctaag tcaaaagaat
    13921 ttgtcagcgc ctttatatag ctttcttttt cttctttttt tactttaatg atcgatagca
    13981 acaatgattt aacactagca agttgaatgc caccatttct tcctggttta atcttaaaga
    14041 aaatttcctg attcgccttc agtaccttca gcaatttatc taatgtccgt tcaggaatgc
    14101 ctagcacttc tctaatctct tttttggtcg tcactaaata aggcttgtat acatcgcttt
    14161 tttcgctaat ataagccatt aaatcttctt tccattctga caaatgaaca cgttgacgtt
    14221 cgcttctttt tttcttgaat ttaaaccacc cttgacggac aaataaatct ttactggtta
    14281 aatcacttga tacccaagct ttgcaaagaa tggtaatgta ttccctatta gccccttgat
    14341 agttttctga ataggcactt ctaacaattt tgattacttc tttttcttct aagggttgat
    14401 ctaatcgatt attaaactca aacatattat attcgcacgt ttcgattgaa tagcctgaac
    14461 taaagtaggc taaagagagg gtaaacatga cgttattacg ccctattaaa cccttttctc
    14521 ctgaaaattt cgtttcgtgc aataagagat taaaccaggg ttcatctact tgttttttgc
    14581 cttctgtacc gcttaaaacc gttagacttg aacgagtaaa gcccttatta tctgtttgtt
    14641 tgaaagacca atcttgccat tctttgaaag aataacggta attaggatca aaaaattcta
    14701 cattgtccgt tcttggtatg cgagcaatac caaaatgatt acacgttaga tcaactggca
    14761 aagactttcc aaaatattct cggatatttt gcgaaattat tttggctgct ttgacagatt
    14821 taaattctga ttttgaagtc acatagactg gcgtttctaa aacaaaatat gcttgataac
    14881 ctttatcaga tttgataatc atagtaggca taaaacctaa atcaatagcg gttgttaaaa
    14941 tatcgcttgc tgaaatagtt tcttttgccg tgtgaatatc aaaatcaata aagaaggtat
    15001 tgatttgtct taaattgttt tcagaatgtc ctttcgtgta tgaacggttt tcgtctgcat
    15061 acgttccata acgataaacg ttgggtgtcc aatgtgtaaa tgtatcttga ttttcttgaa
    15121 tcgcttcctc ggaagtcaga acaacaccac gaccgccaat catgcttgat tttgagcgat
    15181 acgcaaaaat agcccctttg cttttacctg gcttggtagt gattgagcga attttactat
    15241 ttttaaattt gtactttaac aagccgtcat gaagcacagt ttctacaaca aaagggatat
    15301 tcattcagct gttctccttt cctataaatc ctataaaata ggttgtttaa ttaacttggt
    15361 ttgctttttc attcaactgt ttcaatattg catgttttga aaaagatttt tttcctttat
    15421 aagtcaattt ttttccacta atcgaataaa ttattttgtt attttctatt aacttatata
    15481 tataatcttc cccctccgaa gaaaaatact tatctgattt tgtttctaag tagatatttc
    15541 tcttttctaa ctctttctta aacgtttcta gtgtatagat atttgctaat tttcttatct
    15601 ccaataaact attttttata taagttttac attcatcatg attcatacaa actccacctt
    15661 ctataaatga atacaaaaaa agcaatcaaa cgatttccga ttgattgctt aacaattctt
    15721 aaattcagta gcttagatac ttgaaaactc tctgatttcc ctatataatg atagtacggt
    15781 tatataccgt cttcaaacaa agttaattaa ataacttctt acgagggaag agttcatctg
    15841 actaactgat aagcgttggt ttggcaatct tatcgggcta tgcatttata aaatgtcgtc
    15901 aaacatttta taaatgtgtc atggctcttt tttcgtttct attcagttcg ttgtttcgtt
    15961 atatctagta taccgctttt aaaaaaaata agcaacgatt tcgtgcatta ttcacacgaa
    16021 gtcattgctt ttttcttctt ccatttctaa atccaatgtt acttgttctg attctgtttc
    16081 tggttctggt tctgttggct catttgggat taaatccact actagcgttg agttagttaa
    16141 ctttgcaatt tgttctagtg tttttatggt tggatctgat tttcctgatt ctattcgtga
    16201 ataatttgat ctactcattt ctaattcttg ggagaatttt tcttttgatt tggtacctct
    16261 catctttttt aagctttctc taaatgctag ttccaagttt ctcccccctc tttttgtgta
    16321 ataaaattca cgctttattt aaattatagc ttttaatatc ttaataaaca agtgattaat
    16381 cacttgtgat ttgtgattat tccaaaagta ggtaatttca ccaacctttt tcaaaagttt
    16441 ttatcgacga ttattggact ggatcgtgtt tcctaaagcg attctaagcg catgtttaat
    16501 tttaaagtgg gtaattctct ttaaaacgtc ctacaaagat tcaaaaaaag catggctgct
    16561 agtgggggta ccgtcaggaa aatgcggatt tacaacgtta aggaaattta ttaaagagac
    16621 caattcctca gcactaaaat tgataattgg gctttctttg ttactatagt gttaggtaag
    16681 ctttttactt tatcaccggt tttcccctca cttcacacct acgggcgagt ttccccacat
    16741 cccgttttcc atctaatcca attcattcaa tatctttatg tgtatgaaag atcagattgc
    16801 ttgatttaga catcttacac gtaatttatt cttaaatggg tacggtaaac gagcaataat
    16861 acgagcaaaa gtatacctac aaaaataata actctgtcta attttttttg attcatatgc
    16921 ttattgctta aaccatataa tttcagtcca agaaagcctc caacagtatt tgtaattaca
    16981 tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa ttatttcaaa agtaagactt
    17041 aaaaccagta caaaagcaaa cttaggtaaa aatccgattt ctttaaaatt gacattcaaa
    17101 agcaagccaa atggaataaa gattataaca ttatctatca tctctctgaa attcccagta
    17161 gcagtaaatg gagtcaagtt aagacttctt tgatgataat taaatactga taaaatattg
    17221 tattgtaatt tgaataacac taaccagatt agtgtcacta aatataaagc tagcaatcct
    17281 ctagataata tttttcccaa tataatttcc tctaattctt ttgtttaagt tgtatcatta
    17341 gtatagcaat gtcctagtta acgtgggttt taaattaaaa agctaacgtt accccaaaag
    17401 tgctagtaca ataatgctgt tcattaattt cgttggtttc attccgccat ccttacctcc
    17461 ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat tacaccgtct atattgtttc
    17521 ctgatatctc ataacgaaga ttagtcggca catgaatggt ggtattttta gtaacaggat
    17581 aataaaagat ctcatatttt tccccattta cactaacaga aatggttttt tcttctttta
    17641 ggtaatccat atattcctcg agaacgaaat tcttttcttt cataatcgca ctatgtggta
    17701 aaccaacata gcgaatatgc catggttcat attgaattcc tgttaactct gttttgtcct
    17761 ctggataacg taaaatgaac ccgtatttcc aagcattttc ttctatccac tttccttcag
    17821 gggctcgttc cattttcgtc aagcttgatc ctacatctag tgataaacct gaattatgct
    17881 cactataacc tgctggtaag gcatactcag cccccatttc ttggtaaagc acactttgct
    17941 catcaaagtc tcgatagcca ctattaataa taaaatgact aacgccaccc tttacagcat
    18001 cattgaccat ctctgaaaat ttttgtgcta tttcttttga catataaata ttactatcaa
    18061 gcaacccgta tccatttatt aattcgtcat gtttagataa attcacgata tctgacttca
    18121 cactttcttg gcgaacagga tatttactat tgattaatag cagatttcct tgataaacct
    18181 gttcttctgt aattgttttc tcttgggtat tttcagaagt cccactattt tctaaatgtt
    18241 ctttgggatt ttgatcataa ttttgaaatt cgactttttc ctgagaaaac agtgcttcat
    18301 taacgtagtc ataacctaag tatattaaga ataacaataa caataaaaaa aacaacttct
    18361 tcattttcag tctcctcctt aactatatag ttggaagtat gaaatatttc tagaatcttc
    18421 cccttaaacg gcacaatctc gtttgagatt gtgccgtttt gccccgtaaa ttacagtcaa
    18481 ttatttcgcc ctactggatt tactattatc accaatgtag atgaattttc ataagtgata
    18541 cccatgctga atttaacgag gctgaactgc ctgttgacaa tg
//
LOCUS       DASOUD010000053        18258 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.053, whole genome shotgun sequence.
ACCESSION   DASOUD010000053 DASOUD010000000
VERSION     DASOUD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.053"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..1141
                     /locus_tag="U4A29_001647"
     CDS             2..1141
                     /locus_tag="U4A29_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO0680772.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1134..1934
                     /locus_tag="U4A29_001648"
     CDS             1134..1934
                     /locus_tag="U4A29_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO0680773.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1934..2761
                     /locus_tag="U4A29_001649"
     CDS             1934..2761
                     /locus_tag="U4A29_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO0680774.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2739..3473
                     /locus_tag="U4A29_001650"
     CDS             2739..3473
                     /locus_tag="U4A29_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO0680775.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3573..4439
                     /gene="rfbA"
                     /locus_tag="U4A29_001651"
     CDS             3573..4439
                     /gene="rfbA"
                     /locus_tag="U4A29_001651"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO0680776.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKISVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4453..5025
                     /gene="rfbC"
                     /locus_tag="U4A29_001652"
     CDS             4453..5025
                     /gene="rfbC"
                     /locus_tag="U4A29_001652"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEO0680777.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5047..6075
                     /gene="rfbB"
                     /locus_tag="U4A29_001653"
     CDS             5047..6075
                     /gene="rfbB"
                     /locus_tag="U4A29_001653"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO0680778.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6173..7024
                     /gene="rfbD"
                     /locus_tag="U4A29_001654"
     CDS             6173..7024
                     /gene="rfbD"
                     /locus_tag="U4A29_001654"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO0680779.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7059..9092
                     /locus_tag="U4A29_001655"
     CDS             7059..9092
                     /locus_tag="U4A29_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680780.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9136..10416
                     /locus_tag="U4A29_001656"
     CDS             9136..10416
                     /locus_tag="U4A29_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEO0680781.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10626..11432
                     /locus_tag="U4A29_001657"
     CDS             10626..11432
                     /locus_tag="U4A29_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680782.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11444..12664
                     /locus_tag="U4A29_001658"
     CDS             11444..12664
                     /locus_tag="U4A29_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680783.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12654..14240
                     /locus_tag="U4A29_001659"
     CDS             12654..14240
                     /locus_tag="U4A29_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO0680784.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14279..16417
                     /locus_tag="U4A29_001660"
     CDS             14279..16417
                     /locus_tag="U4A29_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO0680785.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(16485..17444)
                     /locus_tag="U4A29_001661"
     CDS             complement(16485..17444)
                     /locus_tag="U4A29_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEO0680786.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            17779..>18258
                     /locus_tag="U4A29_001662"
     CDS             17779..>18258
                     /locus_tag="U4A29_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO0680787.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     "
BASE COUNT         5732 a         3017 c         3840 g         5669 t
ORIGIN      
        1 tatgcttgtt tcttttagta tggttataag attttttctg acaattttat tatcactgat
       61 attaacacct gtattcaaaa tcatttcagt acaaacaggt atggtcgaca aaccaaatga
      121 aagaagaata aataaagttc caatgccttc agcgggcgga ctgcctatct ttatttcatt
      181 tgttatatcg actttattct tattccgtaa aattatccct caatcgtata ttgtaccgat
      241 cttgattgct tctttagtaa tcatagtcac aggagtgctc gatgataaat atgagctgac
      301 acctaaacaa aaaagtatag ggatcttgat tgcttctctt attatttatt ttgtagcaga
      361 tatacgtatt gattccttca cgcttccttt tattggatat atccaactag gctggcttag
      421 ttttcccgta acgatttttt ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg
      481 tttggatggg ctggcggcag ggatttcatt gatcggttta attacgatag gaatcattgg
      541 atattttttc ctccatgctt ctacagtgta tattccagtt gttattttct gtcttgtagc
      601 aagtattatc ggtttttttc cttacaattt ttatccggca aagatttatt taggcgatac
      661 aggagctcta ttcctagggt ttatgatggc cgtcctttca ctacaagggc tgaagaacgt
      721 gacttttgtt tcatcgattt ctttgctgat cgtcatggga gtgccggtaa cagacacgtt
      781 ttttgctatc attaggagaa aagcaaaccg tgtttctttt tctacagcag ataaaaaaca
      841 tcttcatcac cgactaatca gtttaggctt tactcataaa ggtgctgtcc tgacgattta
      901 tgcgatggct ttgatgtttt ctttcacagc tatggtcatg aattatactg gaagaatcgg
      961 aacgatcata ttgattattg ccatgttgtt tgctgcaatt ttattatttg aattgattgg
     1021 tctgatcaat gagaaacatc agtttatttt aaaaacattg cgatttttag ggaataaaga
     1081 gtaccgtacg caagtaaaac agaaatattt gaagaaatgg aaccgtcata tagatgaata
     1141 acaaattaaa ccagccgatc cttattagta ttgtgacggc aaacagtgat aaaatttttc
     1201 ggacactaga taattttatt tcaactgtaa aagatgaaac agcagtaaaa atccgaattt
     1261 ttgacaacaa ttcacttccc gagtacaaaa aaagactaac agagtatagg aaatatccct
     1321 acatcgatat ctattttcat gatgaaaatt ccggatttgg ttatggacat aatcacaatc
     1381 tgatagctag cgattctcgc tatggcatta tttgtaaccc agatatttta gtggatgaag
     1441 agacagttac agcattggtc ttgttattga aagaacatcc ggaatgtgcg atgttagcac
     1501 ctaagattct taatgaagat ggaacaactc agcacctcat ccgtgagcag ctggctgttt
     1561 ttgattacgt tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac
     1621 tggctgtgtt tgaatgccga ggattgccta ctgatgctaa tagttatgta cggatgatct
     1681 caggtagttt tatggtagct gatattgata aatttaaaga gataaagggg tttgatgagc
     1741 gtttcttcat gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca
     1801 agttattgta cacacccttt tatgcagtta tccattttta cgggaagggt gcgcatcgaa
     1861 atgcaaaact gtttcgcgtg tttttgactt ctatgatgaa atttttcaat aagtgggggt
     1921 ggcgtttttt ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc agcaactatt
     1981 cagtcaatct ccagcttatg gaccattaag caaagcactt agcgaaaagc aaattcagct
     2041 tattatttac gataatagtc ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat
     2101 cgtttatcat catgatccgc aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc
     2161 tcaagcagat gaaggaacac gttgtctcgt cactctggat caggatacga ggctagtcga
     2221 cgattatttt gaactattgc gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc
     2281 gatgattttt tctgggagcc gacaagtttc tcccctatat tcagatcgtt atatcaatcg
     2341 tacagctcaa gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg caatcaattc
     2401 aggtgttgcc tggtctgtca aatttctaaa aaagattggc gggtttaatc ctgctttttc
     2461 tttagatttt ttggatcatt ggctgttttg gaaagtggat cagttggaaa aaactgtgga
     2521 gatccttcct gtacgaatgg aacatgatct ttcggtttta gactatgaaa aagtttcaag
     2581 taatcgatac cgttctattt tacaagcaga aaatcgattt tatcatgaat atgatcaaag
     2641 gcatttatcc agtcaccgac gacaattatt gttaagaaca gctaaacagt tcttgaccgt
     2701 caaaaaccgg catatctggc ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg
     2761 aattcgtgcg ttcggtttgt atagctacct ttaatggagg cagatattta agagagcagt
     2821 tagatagtat tttatctcag ctttcagcag aagatgaagt agtcatttct gatgatgggt
     2881 cagtagatga tacatgggat atattaactg aatacgcaga aaaagataag cgaatccagc
     2941 ttttttcggg gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc cagcagacga
     3001 aaggagatct aatctttttg gcagaccaag atgatgtttg gttggagaca aaagtgaaaa
     3061 agatcactga ctattttgag cagaatccgg atcagctagt agtagtttct gatttaatca
     3121 ttgtggacag cgagctgcaa gagatccatg attcgtattt ttcgtttcga aattccaaag
     3181 aaggatggct taataatgtg ataagaagtc actatattgg tgctggtatg gctttccggt
     3241 cttctttaaa acccgatatt ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga
     3301 ttggtttgct gggaggaaag aagactgggt tcctaagaga accgttaact ctgtaccgaa
     3361 gacatgattt aaacgcgagt gagattgaaa cacattctaa attacggcaa aaaatttctt
     3421 ggcgtgttca tctgattacg gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga
     3481 tgtttaaatc cttaatattt ccatgacaaa tataacaatt gatgagataa taacaaagtg
     3541 aaatttgaaa tcaaaaagga gagcagatat ccatgaaggg aatcattctt gctgggggca
     3601 gcggaacacg tctgtaccct ttaacaaagg caacatcgaa acaattgatg ccgatttatg
     3661 acaaaccaat gatttattat ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc
     3721 tgattatttc aacgccacaa gatacaccac gtttcaagga attatttgga aacggaaatg
     3781 atttaggact gcagatcgaa tatgcagtac aagaaagtcc agatggattg gcacaagcgt
     3841 ttatcattgg agaagaattt attggagatg atagtgtctg cttggtatta ggggacaata
     3901 tttactatgg tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg
     3961 cgacagtatt tggctatcat gtcaatgatc cagaacgctt tggtgtagta gaatttgatg
     4021 agaatatgca agctttgtcg attgaagaaa agcctgctca gccaaaatca aattacgctg
     4081 taactggcct ttatttttat gacaatgagg taatttctat cgctaaaggt atcaaacctt
     4141 cagaacgtgg agaactagag attacggatg tcaataaagc atatttggat aaaggaaaaa
     4201 tatccgtaga acttatggga cgcggatttg cttggcttga tacaggaaca catgaatcat
     4261 tgcttgaagc atctaccttt atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt
     4321 gtttggaaga aattgcttac cgtatgggct acatcgataa ggaacaattg ctggcccttg
     4381 cacagccatt gaaaaaaaat caatacggtc aatatttatt gaatttggcc gctgaataag
     4441 gagtccttac agatgaaagt aacagataca aaattaacag atgtcaaaat cgtagaaatg
     4501 gacgtctttg gcgatcatcg cggtttcttc acagaaagtt attcaaaagc aaaatttgca
     4561 gaacatggat tgaaatatga ctttgttcaa gacaaccatt cactatcagc tgaagctggt
     4621 gtccttcggg ggcttcattt ccaaaaagga gaagctgcac agacaaaatt gattcgggta
     4681 gtcactggag cagttttaga tgtagtagtg gatatccgca aaggtagccc gacttatggt
     4741 caatgggacg gatttatctt atctgaacat aatcatcgac agttgcttgt acctaaagga
     4801 tatgcccatg gattcgttac tttgacacca aatgtgaact tcctttataa gtgtgataat
     4861 tactataatg cagaagcaga tgggggcatc gcatttgatg atccagattt agcgattgat
     4921 tggccaattg atccatcaag agcgattatg tcggaaaaag atcaccgtca tccgacatta
     4981 aaagaatttg aagcagaaaa tccatttgtt tacggagaaa tctaagaatt aaggagaaat
     5041 tttcttatga aaaacatcat tgttaccgga ggtgccggat tcatcggctc taatttcgtt
     5101 cattacgtgg taaacaatca tccagaagtg catgtcactg ttttggataa actgacttat
     5161 gcaggaaaca aagagaactt ggccggactt cctagcgatc gcgtagaatt ggtcgtagga
     5221 gatgttgcag atgcagaatt agtggaccgt ctagtaaaag aagcggacgc agttgttcat
     5281 tacgcggcag aatcccataa cgataattca ttaaaagatc ctttcccatt tgtacaaacg
     5341 aatatcattg gtacgtatac acttttagaa gcttgccgca agtatgatgt acgctatcat
     5401 cacgtatcta ctgacgaggt ttacggcgat cttccattac gtgaagacct gccaggacat
     5461 ggggaaggcg aaggagaaaa attcactgcg gaaacaccat ataatccttc aagtccatat
     5521 tcttctacaa aagctggttc agatctgtta gtaaaagcat gggtgcgttc ttttggctta
     5581 cgtgcaacca tttcaaactg ttcaaacaac tatggtcctt atcagcatat tgaaaaattt
     5641 attcctcgcc aagtaacgag tatcttaagc ggtattcgtc caaaattata tggcgaagga
     5701 aagaatgttc gtgactggat ccatacgaat gatcattctt cagcagtgtg gctgattttg
     5761 acgaaaggca gaattggcga aacctatctg atcggtgcag acggtgaaga agataataag
     5821 accgtgatgg aattgatctt agaaatgatg ggccaaccaa aagacgcata cgaccatgta
     5881 aacgaccgcg cagggcacga tttgcgttat gcaatcgatt cgacaaaatt gcgagaagag
     5941 ctaggttggc agcctgagtt tacgaacttc cgtgatgggt tggctgacac gatcaaatgg
     6001 tacgaagaac atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc
     6061 aaaaatggac aataatacag aggaaatttc actgtattaa tggaaaaaca catgtgaggc
     6121 aggcgctcgc ctgtctcttt tttaaaataa aaatcaagga gcggataacc atatgttttt
     6181 attaactgga ggaaatggac aattaggtac agaattgcgc catttattag atgaaaaaaa
     6241 tctgccatat gtttcaacag atgcaaaaga cttggatatc acagatgctg agaaaacaat
     6301 ggcttatatt acagaattaa aacccgatgt tattttccat tgcgcagctt acacagcagt
     6361 agataaagca gaagaagaag caaaagaatt agatgagaaa atcaatgtcg atggcacaag
     6421 aaatgtagca cttgcagcaa aagctgtgaa tgcacaattc gtctatatca gtacagacta
     6481 tgttttcgat ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa cgaatccact
     6541 aaatgaatat ggtcgtacga aattattagg cgaaaaagca gtagaagaga ttttagaaga
     6601 ctattatatc atacgaactt cttgggtttt cgggatctat ggacacaatt tcgtcttcac
     6661 gatgcaacgc ctagcggaaa cccgcgacaa actgactgta gtaaatgacc agtttgggcg
     6721 accaacttgg acacgtactt tggcagaatt tatggtatat gtcatcgaag aaaaagcacc
     6781 atttggtatt tatcatttgt cgaacgaaaa tagctgttct tggtatgagt tcgcaaaaga
     6841 aatattgaaa gatacagatg tagaagttgc acctgttacg tcagaagaat ttccgcaaaa
     6901 agcaacacgt ccgcagtatt cagtcatgag cctgaaaaag acagaagcgt taggttttgt
     6961 cataccaact tggcaagaag cacttgcgca aatgctggaa gctgccaaaa aaatgaataa
     7021 gtaatttcct ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg
     7081 cacgacgaaa agaaaagcca aaagcgaatc aggcagattc gaatttggtg cttgacctgt
     7141 ttctaaaact ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt
     7201 taaacggaag ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa
     7261 aaactgatgt gatttttgga cagaatcggg aagtgcggtc agacgaatgg ctggtgcaga
     7321 caccttttta cctctcacaa gctgaagagg acttttcgtt ggtgaacgag gattattcat
     7381 taaacggaca aaacatgatc attgcgtata attctcctgt aaaggatatt acggttatcg
     7441 gaaaaccatt caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt
     7501 gggcgatgaa attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga
     7561 agaggaaaaa agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc
     7621 agtggtggtt catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgattg
     7681 ctttttatca ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt
     7741 tggtggttat tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc
     7801 tggcgtactt gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt
     7861 catgggatct gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca
     7921 tggttcattt ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc
     7981 caggaaaacg ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta
     8041 caaattggaa aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt
     8101 tctatcactt ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag
     8161 atagcgaaca aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt
     8221 ggattaccgt tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc
     8281 cattaagagc catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg
     8341 cttatatttg gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa
     8401 atgcatggtc ttatttctat gcgattacgc attcgaaaat ggataattat ttttcggatt
     8461 ttgaaaccat cgcagttgct gtgttgggaa cagtgatttt agcttttgca ttatttggct
     8521 ggagacgctt gttttacctc gcttttgtcg ccttagttat cgtatctggc tttttcgtca
     8581 atccagtcgt agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa
     8641 aaatcgatca acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca
     8701 ccccaacatt gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat
     8761 ggaagaaaat cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa
     8821 gagcttttat cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg
     8881 tcacgttgaa ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac
     8941 cattaactga ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata
     9001 aagacggtta tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac
     9061 aaactcagga aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata
     9121 ttagtagaaa ggagagtgaa gataattcaa gcaatcgtac aaaaatttgt caacaaaatg
     9181 agtgtcggtt tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcaggggta
     9241 ggttttccag caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg
     9301 gctattttgc tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt
     9361 gttgcgcttg cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta
     9421 tttggagact tactgtttcc tttgattatt ttattttgtt tagtaattaa atggatcgag
     9481 tggacaaaat gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg
     9541 ttatatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc
     9601 aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt
     9661 gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgttcg aatcatcggt
     9721 gggatagcag tcgttgcagc ctttttaggt atttgggtct gccaatctag aggtgcatta
     9781 gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt
     9841 cgtgcgccat ttattgccta cacggttacg attcttttag ctttgccgat ttcttatctg
     9901 gcggctgtct ctgaaaaagt caatttgttt accggtcgag aagatatttg gcataaattc
     9961 tatcagacgc taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc
    10021 cagcgaggaa accaattttt aggaaaccac aatagttata attctatttt gaatatctat
    10081 ggattgatag gatttgggat tgctgctttg ctgcttatcc tttttattgg tcgcttaacg
    10141 ttgaaagcag atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg
    10201 atgcagtcat ttatggagga tacattgact tcatacccat gggttccgat tatttatatc
    10261 ctgttggcta tggccgttca tcgatatgat gaaccagaag ttacggagga attctatgtt
    10321 atggaagagg catatgacga tatgacttat gatgatacat cttatgagga acaaccagat
    10381 agcgctcatt cttccaggat ccaacgctat cattgatagg cacaaaatac gagagaggct
    10441 gtgacaaccg tcgcagcctt ttttggttta ttgcaattgt ctagcatctt ctgtaaaata
    10501 gaaacgttag agaaaatctt gtatcataga aaattaagaa agaaataaga acgcagctcg
    10561 gtaccaaaaa tatggtaaaa tggcacttga ctgtaattca ctgaaaatcg cttggaggaa
    10621 cacaaatgtt taaagatttg tatttattta tgaaggacat ttatcagaac agaaaattac
    10681 tgctacagtt ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct
    10741 tatgggcgtt tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg
    10801 gactgaaagc acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga
    10861 tgattccctg gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat
    10921 atacttattt agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc
    10981 tatccaatct gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa
    11041 atggtatata tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg
    11101 ctttcttgac aggattgact tggctgacgg catcgatcca accattttta cctgatatca
    11161 tgcaattaat tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc
    11221 ctaatcagtt tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg
    11281 ttcaaggtta tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata
    11341 ctttgtattt ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta
    11401 gattgaaacc acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat
    11461 tagactaaaa gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg
    11521 tgaagccctc aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt
    11581 gaatgtagaa ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa
    11641 atctacaatg ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat
    11701 cgaaggaaaa atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg
    11761 ctatgaaaat atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga
    11821 aatggtcgat gatatcattg aatttgctga catcggcgac catatttatc aacctgtaaa
    11881 aacctattca agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc
    11941 tgatattttg attgtggatg aagcattggc agtaggagat cttgaatttc aactaaaatg
    12001 tatggaaaaa tttacagaaa tcaagaactc gggaaagacg attttattcg tttctcatga
    12061 tattagttcg attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gaaaagttgt
    12121 cgaatatggt gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc
    12181 agttaaaaca gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt
    12241 agacgtgacg aaagccgaat tgttggataa agataaaaat ccagtggata tgctggaaca
    12301 aggagaaaaa ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc
    12361 agttttagga attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct
    12421 actagatggt atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga
    12481 aaagttaggt ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc
    12541 aacggttcct ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat
    12601 cggagaaggc atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat
    12661 atgattttga aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca
    12721 aaccaggaag taaagtttta gagtttggtc ctggaaacgg ccgattaacg aaacatttga
    12781 tcggagcaaa acaatgtgaa gtgagcattg tcgagctaga taaggaattg ttcgattttg
    12841 tcagcgagtt tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact
    12901 attatgctgg acaaacattt gattacgttt tgtttgccga tgttttagaa catttggtag
    12961 atcctggaaa aacgttgaag aaagttagag aatttttgaa tgaagaagga gaaattctaa
    13021 ttacattccc taaccttgcg cataattctg tcatgattga tttgttcaat aatcaattgc
    13081 catgggcttc ttatggtttg ctagatgaaa cccataattc tttttatact catgatggct
    13141 tccaaaaagt ttttgaaaaa gcaggattat acatcaatat tgaagattat ttgtacttag
    13201 ctgtaggtga tacagaatta aaatcaactt atgaagaatt gccggaagct gtacgttatg
    13261 atttcaaaat gcgaccattt ggcgaagtct accaatactt cttctcattg atgaaacatc
    13321 cagtagcaca aagttccatt gcgcagccgc aaaattctaa ttatgtgaaa gtattagaag
    13381 taatccagca aacgaaacaa gatgaaataa ttcaacaaat accattcaat aatttcacag
    13441 gggaaaatga gacactaagt ttccatgtat cagaattagc agaacgaatg gtgtttcgct
    13501 tcgcgcagca acctagcttt atcgaattca gcgcagaagc tgctggagaa aaactcacat
    13561 ttatcgattc aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatggtaaag
    13621 atttaccaga gttcttttta acggatattg cagggaaaga agtgactatc cattgtcact
    13681 atcgttttat cggagaattg acaccaacaa tgaaagaatt gctggaaaca atccgtccaa
    13741 tggctgaagt tacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcatt
    13801 atcttattga gaataataca agattagatc agacgttgaa aacaaccata aatcgctatt
    13861 gcacgttgtt ggaaagtgat gaatggacaa tcaaacatag attagggcga cgtaagaaag
    13921 aaacatcgaa aaaaatccaa gaaaaagagc tttctctatg tattgacgaa aaaatctggg
    13981 atgcagaaac aaagatattg aaaatcatcg ggtggggaat cgctaattcg gatcgccaac
    14041 cattgtccta taagttatca gccgaccaat caccattttt tgaagcaatt cctgttttac
    14101 gagaagaagt caatcaagca aaacagctag ctgaaggagc aaaagcagga tttgaactgc
    14161 ggatcctttg cgagaaggaa cgttcattct tagttgaagc agtggctcaa aatggtcaat
    14221 catggatcat tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt
    14281 gcaaaaaaaa gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga
    14341 tttgcttatt gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt
    14401 agaaaaagaa aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt
    14461 atataatctt gatgttaaga cccaaagcgg ttttaaggta cgtttatcag ggaaaatgag
    14521 aggaaaagca atcttagact tccaaacagc aagacatcaa aatggcatag ctgtgaaatt
    14581 aaacggtaaa tatccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt
    14641 actgaaaaaa gggataaatt atgcaaggac tcatggcgta aaaaaagcaa tccgccgggt
    14701 caaactggaa ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcatgagaa
    14761 acctgatctg aaagagcaaa ggaaagaagt acaaggattt gcctaccgcc cattgatttc
    14821 catacttatg cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt
    14881 acttgatcaa acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc
    14941 agctatccga aagtgtctag aatcctatca agcaaaagat gatagaatta aagtcgtttt
    15001 ccgacaagaa aatggacaca tcagtctggc aactaattct gcattggaaa tggcagaagg
    15061 agagtttata gcgctcttag ataatgatga cgaattacct ccatttgcct tatatgaagt
    15121 ggcaaaagtg ctaaacgtac atccagaact ggatttgatc tacagtgacg aagataaaat
    15181 cgatgcagat gggaatcgct ttgacccgca cttcaaagca gactggtctc ccgatacatt
    15241 gatgggaaat aattatatct ctcacttagg cgtatatcgg acaagcattg ttaaggaact
    15301 tggcggtttc cgtaaaggat atgaaggctc tcaagattat gatttggttc ttcgtgtgac
    15361 agaacaaatt cctgcagaac acatttacca cattgaccgt gtcttgtatc attggcggac
    15421 gatcccggga tcaactgcaa gcaatggaga agctaaaagc tacatctatg attctggagt
    15481 gaaagcattg acagatgctc tatctagaag aaatatcaag ggaagcgtac atcctggcag
    15541 aatttctggt ttttatgaaa tcacttatga tgttttgcaa gaagacttgg tcagtgtgat
    15601 cattccaact aagaatggct atgaagatct aaaaacttgt gtggattcga tcatcgaaaa
    15661 aacgtcttat ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat
    15721 gcaagaatta tttgcagaat acaagcatca gctgaaagac cgatttatcg tcgaattaat
    15781 tgacattcca ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa
    15841 atacttttta ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat
    15901 ggtttcttat gcacagtttg atcgaatcgg ctgtgtagga gcaaaattat tttatccgga
    15961 tgatacaacg cagcatgcag gtgtattatt agggatcggt ggcgttgcag gacatgcatt
    16021 gaataactat gatcgaacac attgtggtta ctttggccga ttggtcattg acgtcaatta
    16081 cttagcagtc actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg
    16141 atttgatgaa atattagaag tagcattcaa cgatgttgac ttgtgcttga aagtttatga
    16201 actgggtcgt tacaatgtct atgcacatca agcagaattg taccatttcg aatctaaatc
    16261 aagaggttat gaagatacac cagaaaaaca aagacgtttt gctggggaaa tcaaaaaaat
    16321 gcaagataag tggccttctt atattgctca tgacccattt tataacgata atctaactaa
    16381 agaaggaatc ggcgattttt cgttgagacc tgattgatta aaagaactgt attttcattt
    16441 ttattaatga aaatacagtt cttttaattt aattatatga tttgtcaaat taagctagaa
    16501 taaaatgctt cttgtacata gctcaaaaat atttcgattg gtgttctgta attcaatgat
    16561 tttcttggaa tatgattacg ttgattgctg acactggaaa taaatgtctg attcactttt
    16621 ctaaaatcca ttgatttcgg cagtccatta cgacgcagaa tcccgttaga attctcgttt
    16681 aatgggcgtt gagaaggtgt tccagggtcc gcaaaatata tatcaatatc atgttggtta
    16741 ctaatggctt tccagttaga aaattctttt ccacagtcaa acgtaataga tttaaagaag
    16801 tttttaggga agcgagaaaa ccattgatta agggcagttt caatatctaa tgccttacgg
    16861 ccgttgggtt taatcgtgat aatgacttta gataatcttt caactaaagt aatgacggca
    16921 cttttatgat gaatgccaac gatcgtatca ccttcaaggt gaccaaactc agaattgaaa
    16981 tcaggataat tatcaggtcg atcatggatt gagcgctgat actgttgttt tccccgtttt
    17041 tcctgatggc cattgggttt tcttttacct ttcatcggta gtgtgtcaat atcaaatatt
    17101 cctttagaaa ataaacgata aagtgttctc ataccgcatg aaacaggcct ttcttttcgc
    17161 ccgataatga cgtcaggcgt ccaaccaaga gtgactttct ctttaatgta atctacttca
    17221 tgagcaggta attgaatgac ttttctacca cattttttct tattttcttt atactggtgc
    17281 caataatcaa gagcagtctt gccttgcttg aacttattga ctacattata aatagtttgt
    17341 atagcacgtc ccattcggtt agcgatttca accggcttat tatgttgaag ataatatgat
    17401 tctatcattg tcagttcgtc tatggtaaga tgtttgtagg tcatttatgg ttacactcct
    17461 ttgttttctt tcgtcggaaa tacaatttga gtgtaccata aatgcctttt tatttttcta
    17521 acttaatttt acaattcgcg ttatatgaat attgtaactt gtgaataagg aattagcagg
    17581 agaatactag ttcagcataa aggaagtgat gggacttctg agacaacttt ctgttatgca
    17641 gaaatacaca tataaacaga caataaacgc ttttccccgt ttcttttaat ttcctttaca
    17701 atttggttat tcactaggtt tttaaacttt gctatggtaa gatgatagag gcattatttt
    17761 atggttagga gtgtatatat gaataaaaat ggggagtgga atgcggcacg cagaatattc
    17821 attattctta tggatgtact tgtattaaat gtatcgatct atcttagttt tttgctgaaa
    17881 ttccagggag aagtaccagt gagaaactta gaaacctttc agcattcagc aatttttatt
    17941 tccatcatct ttattgcgtt gaatattttg ttaggagctt atgtattcta taaccgaatg
    18001 atcagtgata ttgtcttcat tactgttatt ggacaagtac taatagcatt ggggatcatg
    18061 gtagttactt ttgctggccg atggtttgca tttccgcgtg gcgtcatttt gatctcgttt
    18121 atcctaggta cgatattact tagtgtttac cggatagtcg tttacaaact gtacttaaga
    18181 gtcagtcgtg ataagaaagt ccttatcgtg ggtttggaga aagatgtcgc accagctatc
    18241 caaaattttc aatctaag
//
LOCUS       DASOUD010000054        17171 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.054, whole genome shotgun sequence.
ACCESSION   DASOUD010000054 DASOUD010000000
VERSION     DASOUD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.054"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1393
                     /gene="uxaC"
                     /locus_tag="U4A29_001663"
     CDS             <1..1393
                     /gene="uxaC"
                     /locus_tag="U4A29_001663"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO0680788.1"
                     /translation="FLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNNF
                     SNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWSH
                     LELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADDL
                     HYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVLG
                     QRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETLI
                     HLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAEE
                     TDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQFA
                     DGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQAI
                     SYTNAKNYFGFFA"
     gene            1552..2235
                     /locus_tag="U4A29_001664"
     CDS             1552..2235
                     /locus_tag="U4A29_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEO0680789.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            2237..3073
                     /locus_tag="U4A29_001665"
     CDS             2237..3073
                     /locus_tag="U4A29_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEO0680790.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            3084..4838
                     /locus_tag="U4A29_001666"
     CDS             3084..4838
                     /locus_tag="U4A29_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680791.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(5114..5455)
                     /locus_tag="U4A29_001667"
     CDS             complement(5114..5455)
                     /locus_tag="U4A29_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEO0680792.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(5523..7694)
                     /locus_tag="U4A29_001668"
     CDS             complement(5523..7694)
                     /locus_tag="U4A29_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEO0680793.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            7863..8762
                     /locus_tag="U4A29_001669"
     CDS             7863..8762
                     /locus_tag="U4A29_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO0680794.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(8833..9690)
                     /locus_tag="U4A29_001670"
     CDS             complement(8833..9690)
                     /locus_tag="U4A29_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO0680795.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(9675..12377)
                     /locus_tag="U4A29_001671"
     CDS             complement(9675..12377)
                     /locus_tag="U4A29_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEO0680796.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(12390..13679)
                     /locus_tag="U4A29_001672"
     CDS             complement(12390..13679)
                     /locus_tag="U4A29_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEO0680797.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            13829..14875
                     /locus_tag="U4A29_001673"
     CDS             13829..14875
                     /locus_tag="U4A29_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0680798.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            14877..17030
                     /locus_tag="U4A29_001674"
     CDS             14877..17030
                     /locus_tag="U4A29_001674"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEO0680799.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4943 a         3553 c         3190 g         5485 t
ORIGIN      
        1 gtttttatct gatgattttt tattaaaaga cgattgggcg aaaaagcttt accattccta
       61 cgcaaaaaac atgcccatta ttgattatca ctgtcactta gatccaaaag aaatttacga
      121 aaacaacaat ttcagcaatc tgacagaagc ttggttagga ggcgatcatt acaaatggcg
      181 cttgatgcga gcttgcggcg tgcctgaaga aaaaatcaca ggaaatgctt ctgattttga
      241 aaaattttta gcttggtgtc aaaccgtacc aaaaattatc ggaaatccgc tttacagttg
      301 gtcacatctt gaattgaagc gtttcttcgg tattgacttg ttgataaatg aagaaaatgc
      361 agagaaaatt tggacacaag caaatgaaat tttggcagct cctgattttc gccgtcggga
      421 aatcgcaaaa aaatcgaatg tagaagtgat ttgtacaaca gatgatcccg cagacgactt
      481 gcactatcat cagttgcttg ctcatgaaga accagatttg aaggttctac cttcattccg
      541 tccagataaa gcactcaata ttgaaaaaga aggatttaat gcatgggtca cactactgga
      601 agaagcagct gaccaaacga tcaccaccta tcaagaactg atagaagtct tagggcagcg
      661 gatagaatac ttccatcaac atggatgtcg tgtttccgac cacgctttag acatcttacg
      721 ctataaagaa gcggatgaaa cagtattaga agagattttt caaaaagcac gagctaacaa
      781 actattatct tcagatgaaa tcgatgctta tcgtacagaa acactgatac atttgaccca
      841 cttctatcat tctcataatt ggacgatgca gctgcacatc catgcttatc gtaactgtaa
      901 tacacaaatg ttggaaaaat tagggcccga taccggctat gatggaatga atgatctttc
      961 tttgactatt cctctgcaaa aattattgaa ccgtgcagaa gaaacggatc agttgccaaa
     1021 gacgatcctt tattcattga atccgaatga ctatcctgcg attttagctt tgatgggctg
     1081 tttccaaaaa gaacagaatg gaaaactgca gttaggttct ggatggtggt acaacgatac
     1141 tcgagctggt atgcgcgatc aattgacaca atttgctgat ggtagtgctt tggggaattt
     1201 tgtcggcatg ctgacagatt caagaagttt cctgtcctac actagacatg aatatttccg
     1261 tcgtgttctt tgtgaatttt taggcgaaat ggtcatgcga ggagaagcac ctgaagatga
     1321 acaactttta ggaagtctag ttcaagcgat tagttataca aatgctaaaa attactttgg
     1381 cttttttgcc taattgatta atagtaacag taacgaatca aaaacaggat agtgaaataa
     1441 cggaatccgt ttatccacgt tttagagaag aagacggacc aaatttgctc cgtcttcttc
     1501 tcttgctgtt ctgcttcatt gcctataaac ctttatgaaa gaagagataa catgcctact
     1561 atccatattg ccggtgattc tactgcggca gaaaaagatg aaaacaagcg tcccgagaca
     1621 ggttggggag aaaaaatctc tgcttatttt tcacccgaga tccagatcaa taaccaagca
     1681 aagaatggac gcagcaccaa atcatttatc caggaaggaa ggttagcccg tctagcttca
     1741 cagtttcagc cgaatgattt cttatttgta caatttggac ataacgacca gaaaatccaa
     1801 gaagaaagag gtacgcttcc cttcggtagc tatttaaaaa atctgcagat attttttgaa
     1861 tgcgcaaaaa aagcgaacgt ccaaatggtc ttactgacat ctgtgacgcg acgagattac
     1921 ttagaaaacg gaacattgaa tccagatata cttggagact atccaaaagc gatgcgtgca
     1981 tttgctgaga aacaccatat tcctctttta gatgtgttct caagatcaca agaattgttc
     2041 cagaccttct caaaagaaga gacaaaaaaa ttctacctgc atcttatacc tgatacttgt
     2101 aaaaattacc cagaaggact aaaagacaat acccacttta gtccagaagg cgctgataaa
     2161 gttgcccgaa taattgttga attgataaaa gaatcccgtc tgcctcttgc taattattta
     2221 caaaaaggag tttgagatgt ttccattaaa tttaggcatc cgtgcccacg atttagatgt
     2281 tcgaaatcgt gaagaaatcg ttcaaaaagt atcacagctt cacttgtccc atatccaatt
     2341 tgcaccacaa aaagcatttc ctgaacaact taaattaact gacatgacac ttgggagtgc
     2401 tgcctatttt ggtgaatact tccaaaaaaa caatatcgaa ctttccattt taggatgcta
     2461 tatcaaccta tccagcaaaa atcaagaagt tcgtgcacaa gcattggcaa ctttcaaaca
     2521 gcaaattgct ctgtgctctg cctatcaagc caagatgatt gctacggaaa ccggtagtgt
     2581 ctctgtcggt tacacaaaag aaaattttac tgaagaagct tatcagatcg ccagaaattc
     2641 tatcattgaa ttagtcgctc atgcagaaaa ttttggcgta actgttgctg ttgaagcagg
     2701 gatcaatcat ccaatctata actatcagtt agctaaacgt ttaatcgatg aagtagcttc
     2761 accaaattta aaaatcattt tagattgtgc gaatttgatg cataaagaaa atcatgctga
     2821 tcaggaaaaa gtgatccgag gtgccttgga caatttacat ggttatatta cagcgcttca
     2881 tctcaaagac tatatcatgg aaaatggaaa gatccgcatg gtacctgttg gcaagggctg
     2941 tttagatttc cgcccggtac tgcattatat caaatatgag cgtcctctaa tgtacgccac
     3001 tcttgaagct actcctgaga tttatgttcc tgaagcaata gaacacttgc agtttattta
     3061 ccaagattgt taaggagaag attatggagg aacgaataca gaaaagttat caaaaatatc
     3121 atgcaaatgc ttttcagaca ttgctgcatc tttatcaggg aaattattgg cgttttgcca
     3181 tgtcaggact cttttttatt atcaaacatc ttccatcttg gttaatgccg atcgctattg
     3241 ccaatgtaat caatgcagct tcatcgaaag attcttctca gttgcaggcc atttatttca
     3301 acgctttttt gatggcttgt ctgattggtc aaaatatatt gacgaattat tttcatgtaa
     3361 aataccatag cacttcgatc cgtcaagtag aagctggact aagagcgacc cttatcaaga
     3421 aaatccaaga actatctatt ccttatcaaa aagaattgca atctggacga atccagtcaa
     3481 agatcatccg tgatgtagaa gctatccaaa cgttgtcctc tcaagtattt gtcagcggat
     3541 taaatatcgc tgtcaattta ttcgtagctt taggtatcac gctttataaa agtcctgttg
     3601 ttttcatgtt tttcttattg actgttcctc tcgccgcatt cgttactctc tttttccgta
     3661 aaaggatcag acagtccaac tcagacttca ggcaagaaat ggaagagact tctgcaaaag
     3721 tgatcgaagt gatcgaatta gcaccgattg ctcgagctca tgggttagaa aaacacgaaa
     3781 caactcgctt aaataagcat tttactcaaa tctatgaaaa aggattccac ttagatatcc
     3841 tgcaatctgt tttcggatca gtcagctggg ctagttttca gatatttcag ctgatttgtt
     3901 tgattttcac tgggatatta gctttacaag ggcgcatcca acccggcgat gtcgtgatgt
     3961 atcagacgta tttcacgacg atcgtcaatt ccgtttctgg aatgatcacg ctgattccta
     4021 cgatttcaaa aggcatggaa tctatcactt ctattggcga actgttgatt gctggcgatg
     4081 tagaaactta tcacggtaag aaaaaaatcc aacatttgga tggaaatttt cgttttgagg
     4141 atgtttcttt tctttatccg gactcactca aaccgattat cgatcatttt tctctagagg
     4201 tgaaagctgg agaaacaatt gcttttgttg ggccatctgg ctctggaaaa tctacactcc
     4261 ttaacttatt gattggattt atccagccga cttcaggaaa aatttttcta gatgatcagc
     4321 ctttggatga actggatatg cgaagtgtgc gaaattattt agccgttgtt cctcaaacca
     4381 ctttgttgtt ctccgcttcg atcaaagaga acatcactta tggcttgaaa aacgtatcaa
     4441 aagaacgttt agatgaagtc atcgaagctg cgcaactttc ttcactgatt gaccagcttc
     4501 ctgatggtct ggatacctta gtaggcgaac atggcaacaa gctgtctggt ggccaaaagc
     4561 aacgtatctc cattgcacga gcgttgatac gtcagccaaa aatcattttg ctagatgaag
     4621 caacctcagc tttagataac cagtcagaga aaaaaatcca gcaagccctg aattacctga
     4681 cagaaacacc cactactttt attgtggctc atcggctttc aacgatcaaa gaggcagata
     4741 aaatcgttgt gatcgatgaa ggaaaaatcg ttgaaatagg tacttacgaa aaactgttag
     4801 ctcaaaaagg ctatttctac cgcttgaaaa atgaatgaca aaaaatagga gctatgacaa
     4861 gacttttgtc gtagctccta tttctgaata aacggtgttc tcctgccaac tcctcggtca
     4921 caagtcacaa tagtcatcaa acatgagaag ctgttttttg aaattgttcc ttgtaactca
     4981 gagttaaacg gtaggctcac gacctcttgg taaatggtgt tcaagagaca gcctatcggc
     5041 aataagaagc aattttgtca tcgctcaagg ccgaacgtct ctttcacacc ctctgttgtt
     5101 tcataaaata gttttattcg ctgtaaaggt cgcgaactgg tgtcatgatg atacggttca
     5161 agtcattgat gattgtactg aagccttgct ctttttgcat caagtcattg attacttctt
     5221 ctgattggat tttcacagcc atttcttgtg ctttcattgc atcttcgtcg ctgaactctt
     5281 gtccactcat ttgtttttct tgtaattcga tttgcatcgc ttggaagtct ttgaacagtt
     5341 tgtgtgcttc ttcgtttgct tgtactttct cgtacgcttc tttcaacgct ttgaattctg
     5401 gtaattcacg gatctcacgt tcgatttgat tcgcgctatc gtagatgttt gacatgttat
     5461 gccctctttt cttttagtag ttactctcta ttgtaacatg gggttattcg ttttcaaagg
     5521 ggctactctc cttgatattt ccgccagagg tctttagccg cttcaccaat tccctgaacg
     5581 ccttctttca ccatatcgcc gaagtttttc agcccttctt ttgcttgttc accatattga
     5641 ttcaagttat cttgccaatt tccagactct ccattagaat ttcctgtgtt tccaacttga
     5701 tctgcagcta ccacttgtcc tcctgtggca taggcatctg ctacactaaa tcctgttttt
     5761 gaagtatacg gaaggatgcc ttctgccgct gatttgaaga ctttcccgac aacatttcca
     5821 cttgtccctt ccaaatagtg cagcttgctg gattcttcgt aacccatcca tgtcgaaatc
     5881 actacgtctg gcgtgtaacc gatcatccat tggtcatttg cttttgtggc atcgaaatta
     5941 gtttctgtgg ttcctgtttt ccctgctatc gtataacctg caggattcgc agcagccgct
     6001 gttccatttg aaaatgtacc aagaagcatg ctggtcattt catcggctgt ttcctttgat
     6061 atgacctgtt ttggtttttt gtttgtgtta tcgacgatca ccgctccagt cgaatcaacg
     6121 attttcgtga taagatgcgg cgtatacatc tgaccatcat tggcaaatgc actgtaagca
     6181 cccgccatga tcaatggaga tactcctttt tccagtccac caagggctaa gccataatac
     6241 ttatcagatt ttgctaaagg caggccgaat tcttctgctt tatcatatcc tttttgtaaa
     6301 ccgatctcat gcaaaagcca tacagtaggc aaattcaaac tttgtgcaac agcttgatac
     6361 atcggtactt ctccttggta tgtcccgtca aaattatgcg catcgtaata actttgcggt
     6421 tcgtctttca agatactgga aggcttgtat ccagcttcca gtgctggtgt atatacgctt
     6481 aatggtttga tcgttgatcc tggtgaacga cgcatctgtg tagcaaagct gaatcctcta
     6541 aagacatgat ctcctcttcg tcctactagt gcttgtacac cacctgtatt cggatctaaa
     6601 gctactgaag cagattggac catggcacca tctgaagcat ttgctgggaa taatgcactg
     6661 ttttgataag tagcttctaa ttgcttttga taatcctgat ctaaagctgt atagatttta
     6721 tagcctttat tcatgacatc ttcttcatca agaccatact cgttaactgc ttcatcgatc
     6781 actgcatcaa aataataagg ataacgataa ccagaatctg aattatcgta tgtgtcatta
     6841 agatacgcgc tgatatctgt ctggctttct gcttgttctt gttcttttgt gatttttccg
     6901 ttttcttcca ttacacttag tactgtgttt ttccgatcat tcgcattttt tgggtgatcg
     6961 atcggattat aaataccagg acctttcagc attcctgcca aagtagcagc ctcagacagc
     7021 gtaacattcg ttgcatctac tccaaaatac tttctggaag cgtcttggac accccatacc
     7081 ccgttgccaa aataggcatt gttaagatac atggtcaaga tttcttcttt gctgtacttc
     7141 ttttcgatct cgattgccaa aaataattcc ttcgcttttc gatcaaacgt ttggtctaat
     7201 gtcagatacg catttttggc aagctgctga gtgatcgtac ttcctccgcc ccctgatgtt
     7261 ccgccagaca agaccattcg gactgctgca cgggcaatcc ctttgatatc aaacccgtga
     7321 tgctcgtaaa aattcctatc ttctgtcgag attactgcat cttggatata gggagaaatc
     7381 gcgtttaatt ctacatatgt tcctttttgg gcgtacagtg tgccagcttc ttcgtttttc
     7441 ttatcataaa tgactgtcga ttggcttaat cctgatttta acgtttcgac atttgcttgt
     7501 tttgctaaaa taaagaggta gatacttgtc actaagacac agatcaaacc aatcagcaaa
     7561 atcaatttgt tcacatgata ttttttccag atgcgctttc gccatcgatg gaattgtccg
     7621 agatatggtc tgatccaacg ccaaaaactg atcaaagcag cttttatctt ttctaaaatg
     7681 ctttgaaaat ccatttgctt cttctcctgt ctatttgatt caatcgtctt attttaccat
     7741 agtgaaagag tgttttcttc ctgcttaagt tgtagttcaa tatgtttttt tacgttagtg
     7801 caagtagagt aacataagaa tgaacagaaa aatgtaagtt ttttcttaaa tatgttttaa
     7861 aaatggtaca attagtctgc gattgcactg aaaggagtac acttgtgaaa atcacgatca
     7921 ctttacctat gaaccatcct gaaacaacaa ttcgcgactt gcttgaaaag gaatggttag
     7981 ttcctagaaa ggttcgacac tttttgcgta tacgtaaaaa cgtttggatc aatcaacaac
     8041 ctgctttgtt ccatcatgaa gtaaagggca atgaccagat cacattgtta ttcgaagaaa
     8101 cagattataa ttatcaagaa gttcaattag gagatgcgaa gcacatccgt gttttatatg
     8161 aagatgagca tttgattata gtggataagc ctgcaggaat gaagactcat ccaaatgaac
     8221 cgacagaaaa cagtacctta ctcaatcatt tagctgctta tctcgacact aaacagcaac
     8281 gaccttacgt tgtccaccgc ttggacaaag aaacaagcgg tgccgtttta tttgcaaaaa
     8341 atccatttgt cttgccgata cttggaagaa tgttggaaca aaaaatgatc tatcggagat
     8401 atcaagccat cgtatgggga gaaattgcca aagatatgac catcaccaat aaaattggac
     8461 gagatcgcca tgatcggaga aaaagggtcg tagatgataa aaatggtcag agcgccttga
     8521 cacatgtcac tgtcaacaaa gtactttctg aacaaacaca agtctattgt gttttggaaa
     8581 ccggacgtac acaccagatc agagttcatt tagctcataa gggacatccg atcgttggag
     8641 accctctcta ccaaaataga acagctgaac gactgatgct ccatgcatta gaactgcaca
     8701 tgatccatcc atttactcaa gaaaaaatag tcggtaaagc tttacctgga ttatgggaat
     8761 agaaataaaa aagaacagag tcttatcagc agataatgat aagactctgt tctttttgtg
     8821 taatttgtct gcttactttt gatgttcgtg ttctgctaac agcaaactac cgataatccc
     8881 actttcattc ggcagtcccc aacgaacgat atattcactt acttgaggtg tttccatata
     8941 accacccatc aattcaacaa attgctgacg gattttttga aggagatgat cctgattcat
     9001 taccccaccg cctaaaataa tcttttcagg tgctaacgtc aacgtatagt tgatcagtgc
     9061 ttgagcaata taataagcct gaatagccca aacaggatga tcttgcggca gatcttgccc
     9121 tttgatccct gtgcgtgcct caatagacgg tcctgctgct aatccttcta aacaatcttt
     9181 atgataagga caagtacctt cataatggtc atctggatgg cgtttcaccc aaatatgtcc
     9241 catttcagga tgtgcgatcc ctgaaaaaat ttcttcattg atcacgacac ctgcaccgat
     9301 tcctgttccc acggtaaggt aaatacaact attttttcct tgtgctgctc cttgcttcaa
     9361 ttccccatat gcagcagcat tgacatccgt tgtccaagca gcaggaatat tgtagcgttc
     9421 cttgattgtg ccaagaaaat cgaattgtgc ccaccctttt ttgggagttg ccaaaacgta
     9481 tccgtaatta tcttttgttt tgtcgatacc aatcgggccg aatgagccaa tcccgattcc
     9541 cttcaattca taacgatcaa aaaattcaaa tacctgatcc aatgtttctt ctggtgttgt
     9601 ggtagggata cttaattttt ctaaaatttc tccaccatct gaaaccgcac acacaaattt
     9661 tgttccgcct gcttctattc cgccatacat tcgttttcct cctcgaatcc gattgtccga
     9721 atctcataag gttggattat tgggacaacc gcttgcttcg ttggttcttc aagcagattc
     9781 aatatgacag tttttccatt gtctttagtt atttccaatt tttctaaatg actagacata
     9841 ttgaaacctc ttactactac tttatcacta aacttgctgc gtttcaaagc tgtaattgca
     9901 aacgtttcac tttttatcgt taaatattgt tgttttgaaa tcaatgttcc ttcatgatgt
     9961 ttgatttgtt gagtggaaaa aggaatctgt gctgcgtatg catgcaaata agtagagaat
    10021 ttttcttctg gtccatgtag ttcaatgctg taattgaacc gatgctcacc tagacactgt
    10081 gcttctggtg ttggaaaata tccccaatcg cctagttctc ccacactgcg aagtagcgtc
    10141 actgcgattt gtccgtcttc tgttacttca tattcattca aaccaaagtt tccgactgtt
    10201 acaccgtact cttcatcatg aatattgaca aatgcttgct gatgttgcgg attcgtgggg
    10261 ttttcccaac ttttagacac atggtttggg cgcttcacca cttcaaaaat actatctgct
    10321 tcgtgatgtt ccacatgtaa ctttgtagga aacaagacac gtaagcgatg atctttcatc
    10381 tgattgtcga gagtcgtttc aaagtcgatt tttttactat cttttctgat tgtcatgatt
    10441 gtttttattt gaaggattcg tttttctttg gatcgttctg cttttcgata acgaaattcc
    10501 acaaccatct gctgttcttt ttctaaacgc tcgtctgcag aaacagggat ttccatttcc
    10561 tgtagaatac ttatttttgc tatttccggt gtatctacta atacttttac ttcagagttt
    10621 tctttgttac tggataggat tggctgatca cagaaaggtt gtttaaagat atactcattt
    10681 cctatatcgc cgatatcttc aaaaatatgc agattattca actttcgttt gtttttgcga
    10741 tcttccattg taatggtacc atttttttca atcgtcaaat gtagagggcc gttttcgatg
    10801 attcgtccac tctgatgaat cattgtttcc ttttcttctg ctttcgcttc cccttctaca
    10861 agcgcgaatg tttcccaaga aaatgctggc atgttttcta atggtaaagt cactttgatc
    10921 attcttgcca tatatggcac acggaaacgg tctttcggca aatcataacc aaattggaca
    10981 gtttcttcag ataaaatagc tggtatagtg gccccacttt ttgtttctac atgatactta
    11041 cgtttaggca aattttctaa ctcttgatat agttgttcag gtattccttc agcgaatctt
    11101 tttctttcta attcgatggt tatttctgct tcaccagttt tatccattcc tgcagtattt
    11161 acaatgacaa atggaaagct gtcttttggg aatccggtcg tatcgattgc tgcggtcagc
    11221 tgttctaagg aatcttgtgc aagatacttt ccaacttcat ctgctttttc aaaacgaggt
    11281 atcatttccc gatggacaga atcgactgaa caaccgcaaa tactgtcgtg aggatgattt
    11341 tgcattaatg tcttccaagc ataatctaat tgatcatggg gatagtttcc actaacttca
    11401 taagccatcg tcgcaagtgg ttctgtaata ttttctagct gtcgctggac cttggtattc
    11461 cattgtttta agtaaacccg agcagatgct gtattggcta aagtatacca gccatccgtt
    11521 tcttgacttg tcaattctcc ttctactgag cctaagtctt ctggcacatc tttttgaact
    11581 gcttccagat agtcagtaaa attagaatga ataaactcgt aatctggaaa cagttcattt
    11641 gcgagatgga ttgcttttga tatatctttt tgtaccggtt gatgatcaac accattcatc
    11701 atcaataagt ggttcgtaga ggcatattgt tctgcatccg ctaatttttg cttccaaaag
    11761 gcaagagctg cttctttttc tactggaatc tcattcccgt tgctatacca attggcaaat
    11821 agcagaccaa agattgctgt ttgatcaggt cctttccacc acatctctga gtattgggaa
    11881 gaatagtttt ctgcttccaa aacttgatta tcaaagccaa ttggttttac accacggcca
    11941 aacgctgctg ctgaaatacc agcttgtttc attaattgag gcgtctgtcc catatttcca
    12001 aatgtatcgg gaaaatagcc taacatgaca ggggttcccc atttacggct ttcttccatg
    12061 ccaatcagca tatttctcac gttcgattcc gagctgataa gaaaatcatc ttgcaagata
    12121 taaaatggtc caattctcaa ttttccttca ttgattgctt gttggattgc ctgtcttttt
    12181 tctggcctta cttgaagata gtcatctaaa ataatcgttt gcccatccaa atgaaaactg
    12241 ttgaaggacg gatcaacttc aaatagctct agtagatcgt ctatcaattc aatcagtcgc
    12301 atgtgatgct gttcgtaagc catgtaccat tcacgatccc agtggctatg agaaatgata
    12361 tatactttct ttttcattta cgatacctcc tacttttcta ctctaatatc aaagtaatcc
    12421 atgactaatt cgcaaaacat catatttgcc caagaaaacc attctctcgt ataattttca
    12481 ggattgttca catcgaatcc ttcgtgcata aggtgtgttc ctgcgtcagt tgctacaagt
    12541 aaattcaata tccgctcttt ttcagatttg tctgacgttg tcattccttc catagctaat
    12601 gcaatcggcc aaacgtattt ttctggcgta tgggaggatc caattccttt tgcgtacttt
    12661 ccttcataga aatatggatt ttctttactc aacaatgttc ttcttgtttg cagataatct
    12721 tcctcttgtt cagaacaata tcccaagtaa ggtgcggcga tcagattggg cacattactg
    12781 tcatccatga ctgtggcgtt tcccattcca tctacttcat atgcataaat cgtttgccct
    12841 ttttgattaa ccgttcgtcc gtgattctga atcccctctt ggatctcttt cttcaatttc
    12901 tgtgtttgct gtacaatttc tttatcatcg agtatcgttt gaaaaatttc ttccacataa
    12961 cctaacacga ccaccgcaaa catattggat ggtaccagat atccataacg gcaagtatca
    13021 tcgctaggac ggaaaccaga ccatgtcatt cctgtaaaag ctgtttttgc acctcgcccg
    13081 tcattgatca gcgtatcttc ttttcttgtc gtgtctcgta caaatgtata aggagagtgt
    13141 tcatgatctt gctctgtttt gaacacgtcc aagatttttt tgataccttc aataaaagat
    13201 tgattgaatt gatcagtttt tcctgtattt ttgtaaagaa gataggccaa ctgtattggg
    13261 tagcataaag aatcaatttc atattttctt tcccagatcc aatcgttcat ttttgtatga
    13321 tctgtttggt ggccagcacc gttagcttct tcgttgaagg cattcgcata tggatcgata
    13381 ttgatataaa aaaattgctt tttgactaac ccgctgatca tgtcagctaa ttcctcatct
    13441 tcttttgcaa tcactaaata aggacgaact tgtgcagtag agtctcttaa ccacatcgct
    13501 ggaatgtctc ccgtcaataa aaaggtggtt ccgtcatttt gctttttgac tgttgtcaac
    13561 agagtattgg caaatgctgc tgtaaaattc tttgcccagt ccgcatggat atccccacat
    13621 ttttgtgtga tatcttccat aaatttctgc accgattttg gtatatcttt ataagccact
    13681 aataaaacct tctttctatt tgatatatct ttaaagatta tacttcatac agccaattat
    13741 gtctacgctt tcaaaaaata tctttttatt tactttttca tccattatgg tattatttga
    13801 atatgatata tcattaagag gtgtttatat gaatcaacca ttgtaccaga caatatttaa
    13861 tgatttgcaa acggctattt taactggtga attacctgta gacagccaac taccaactga
    13921 aaaagaactt tcacgtacct ataaagtaag cagaatcact tccaaacgtg cattgactga
    13981 attagagcaa ttaggactta tttatcgcgt tcgcgggaaa ggcagtttcg ttaaatccgc
    14041 ttctccggaa cagattcttg cttctcctac aaaaacacga cggattctgt ttcttcttcc
    14101 atatgtcgct gatttgtctg ttggtgattt tagcaaggga ttgaaccctg tgatgcaaga
    14161 taaacagatt gatgtcatga tgacgacgct tgactttcta caaaataaaa cagcaaaaga
    14221 gctaatcaat gaatttgacg gattgattta ttatgctctt gatacagaac aacatcttga
    14281 cttacttttt gagctttcat tgaaagaatt ccctgtcgtc atattagata aaaaaatcta
    14341 tgagttacct tttcctaccg ttctttccga taatttccaa ggcggtgcgc ttgctgcctc
    14401 ttatttgatc gaacagggac attcaaaaat cgcttatttg tttggtacgc agactcatcc
    14461 acaatccgtt cgacagcgtt accttggtta ccttgaagca ttggacaaag ctggtttgac
    14521 tttccacact tcactggatg acaaaagcgc gacattagat gaattgcttt cttatatcac
    14581 taagtatgaa gtaactgctt ttgtttgtga aaacgatgtt gtcgctatcc agaccatgaa
    14641 tctcttgaga cagcatggtt atcatatccc tgaagatttt tcaattatcg gatttgatga
    14701 tatccaagca gcagctcttg ttgacccgcc actgacaacc atcgcacaag attttgaaca
    14761 gttaggtaag cttgcgggag atgctttggt cacttggtta gagacaaaag atcgaccatc
    14821 cgatagtaaa tttccagttt cattcatcaa aagacaatca acaaaggaga aataaaatga
    14881 atatcggtca aatagatact agacatgcaa cagcgaatca gccaaatttc tctaatggga
    14941 actgccagcc ttatactggc gttccttttg ggatgaatta ttttgcaccg caaactacgg
    15001 atcaaaacgg cagctggtgg tttcatcctg atgaccgcgt ttttcaaggc taccgtctta
    15061 cccatcaacc aagtccttgg atgggtgatt tcagtcatat gctgtttacg ccgatcaatg
    15121 gaaaactaca ggagaacacc cttttccatg ctcaaagttc ttaccgccca gaagaaagta
    15181 tattttgccc gactcatctg tcgatctgtc aattacgtta tggcatctgt tctacattga
    15241 tacctagcat gtatggcgga atcttatcta ttgattacag caaaaatgac agcggtttac
    15301 ttttatcctt tccgggacgc catcagctct ttgttataga tccttacaca gtggaaggaa
    15361 aaatcacaca ttttgctggt tcagaagatc ctgatttttc tttttatttt atattgagat
    15421 ttgaatatcc tttaacaatt gatgagttag ctgtttccaa caaaaatagc gactcgatcc
    15481 cgttgtattt tgaacagaca aaaaaacaaa ctatccgatt tgggacatct tttatcagct
    15541 cggaacaagc agcttataac ctggaactag aacaaaacca aagcaaagag acttaccttg
    15601 ctcacagtca agaacaatgg cagtcctatt ttgatcgtat acagctcaca caccatgatc
    15661 aacaacaatt gcggacgttt tatcataatt tatatcgggt gtttttattt ccacaaacat
    15721 tttatgaaat tgatcctagt gggcaaaaaa tccactatga tacgactagt cgatctataa
    15781 aacctggtgt tctctataca aataatgggt tttgggatac gtataaaacg gtttatcctc
    15841 tttattcttt gattgctgta gaaaaatacg aagaaatgct ggaaggtttt ttgaacagct
    15901 atagagaaag tggctatttg ccaaaatggc tgtctcctga tgaacgtgga cttatgcctg
    15961 ggacattgat cgatgcggtc attgcagatg ctgctgtaaa agatatccga cgagacttga
    16021 tgcctgaatt tctaacggca atggaaaaag gtgctacggt tcaaagcaaa catccaaatt
    16081 atggacgcca aggaacagaa gattacttga catatggcta tgttcctaat cactaccacg
    16141 aatcagtcaa tcacacatta gactatgctt atagcgattt ttgtattagc caagttgcca
    16201 aggtactaga agaagaggaa atgacgaaga aatatcagca gcaatcattg aactatcaaa
    16261 atatctttga ctatgagtca ggatttatgc gttcaaaaga taaagatggc aagttcagag
    16321 aaccattttc agctaatcgt tggggacaag attatgcaga aggaagtgct tggcaaagca
    16381 gctttgctgt ttatcacgat ttttcaggac tgatcggaaa atatggtggc agtgagagat
    16441 ataaagaaaa actcattgag ctgtgcaatc agcccccaac gtttgaccca ggaggatatg
    16501 gttttgagat ccacgaaatg agtgagatgg ccgcgataga atttggacaa ttagctattt
    16561 caaaccagcc aagttttcac tatccttact tgttcagtta tataggacaa cctgaaatgg
    16621 cacagccact gcttaaacag ttgatgactc agaccttcga cgattctcca atgggttacc
    16681 ctggagatga ggacaatgga agtatggccg cttggtatat cttcaatagt ctgggatttt
    16741 atcctgtcac gcctggaacc ggtgaatatg ttatcggtat gccattaatt gacaaggctg
    16801 ttttgctttt gtcaaatgga aaggaattga cgattgaagc aacgcctaat cggccgcagc
    16861 aacaatttat ccataaaatc aaacggatgg acaaagaata tacgaaatta tattttaccc
    16921 atcaagattt gctgaaaggc ggcacgatct cttaccaatt agggatcatc cctcacccta
    16981 agaaatatca agcaaaagat ttgccttttt ccttatcaaa ctataattaa caagaggttg
    17041 tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaataga tcctcatatt
    17101 tttcgtttgg ttggtcttat taccgcagat gcaagctttt gaacaccgtt tattgagagg
    17161 ttgtgaaatc a
//
LOCUS       DASOUD010000055        17005 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.055, whole genome shotgun sequence.
ACCESSION   DASOUD010000055 DASOUD010000000
VERSION     DASOUD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.055"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..124)
                     /locus_tag="U4A29_001675"
     CDS             complement(<1..124)
                     /locus_tag="U4A29_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEO0680800.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="U4A29_001676"
     CDS             complement(228..539)
                     /locus_tag="U4A29_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO0680801.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="U4A29_001677"
     CDS             complement(541..738)
                     /locus_tag="U4A29_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEO0680802.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="U4A29_001678"
     CDS             complement(1132..2859)
                     /locus_tag="U4A29_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO0680803.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="U4A29_001679"
     CDS             complement(2852..4045)
                     /locus_tag="U4A29_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO0680804.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="U4A29_001680"
     CDS             complement(4232..4876)
                     /locus_tag="U4A29_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO0680805.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="U4A29_001681"
     CDS             complement(4970..5104)
                     /locus_tag="U4A29_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEO0680806.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="U4A29_001682"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="U4A29_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEO0680807.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="U4A29_001683"
     CDS             complement(7235..7708)
                     /locus_tag="U4A29_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEO0680808.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="U4A29_001684"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="U4A29_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEO0680809.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="U4A29_001685"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="U4A29_001685"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO0680810.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="U4A29_001686"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="U4A29_001686"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO0680811.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="U4A29_001687"
     CDS             complement(11755..12894)
                     /locus_tag="U4A29_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO0680812.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="U4A29_001688"
     CDS             complement(13203..13916)
                     /locus_tag="U4A29_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEO0680813.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="U4A29_001689"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="U4A29_001689"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO0680814.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="U4A29_001690"
     CDS             15105..16637
                     /locus_tag="U4A29_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO0680815.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="U4A29_001691"
     CDS             complement(16828..>17005)
                     /locus_tag="U4A29_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO0680816.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DASOUD010000056        16388 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.056, whole genome shotgun sequence.
ACCESSION   DASOUD010000056 DASOUD010000000
VERSION     DASOUD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.056"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..750
                     /locus_tag="U4A29_001692"
     CDS             <1..750
                     /locus_tag="U4A29_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO0680817.1"
                     /translation="GIYSNYSLIVTSVQNLCKQITNSITASVGNFAVSNKKKDSFELF
                     KKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILIVLNYSVQVYRNTSF
                     VFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIGTIGSSLGFVIWYEA
                     FIIYKTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGGSTFLQFITKTLITI
                     VTTSILYLLLYFRREEFTFIKKILFRYILKK"
     gene            807..1922
                     /locus_tag="U4A29_001693"
     CDS             807..1922
                     /locus_tag="U4A29_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO0680818.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            1919..3334
                     /locus_tag="U4A29_001694"
     CDS             1919..3334
                     /locus_tag="U4A29_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEO0680819.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            3367..4314
                     /locus_tag="U4A29_001695"
     CDS             3367..4314
                     /locus_tag="U4A29_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO0680820.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(4354..5514)
                     /locus_tag="U4A29_001696"
     CDS             complement(4354..5514)
                     /locus_tag="U4A29_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO0680821.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            5679..6854
                     /gene="mltG"
                     /locus_tag="U4A29_001697"
     CDS             5679..6854
                     /gene="mltG"
                     /locus_tag="U4A29_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO0680822.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            6990..7469
                     /gene="greA"
                     /locus_tag="U4A29_001698"
     CDS             6990..7469
                     /gene="greA"
                     /locus_tag="U4A29_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO0680823.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            7637..8368
                     /locus_tag="U4A29_001699"
     CDS             7637..8368
                     /locus_tag="U4A29_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEO0680824.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            8365..9432
                     /locus_tag="U4A29_001700"
     CDS             8365..9432
                     /locus_tag="U4A29_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO0680825.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            9439..10071
                     /locus_tag="U4A29_001701"
     CDS             9439..10071
                     /locus_tag="U4A29_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0680826.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            10211..10876
                     /locus_tag="U4A29_001702"
     CDS             10211..10876
                     /locus_tag="U4A29_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO0680827.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            10904..11197
                     /locus_tag="U4A29_001703"
     CDS             10904..11197
                     /locus_tag="U4A29_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEO0680828.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(11265..12212)
                     /locus_tag="U4A29_001704"
     CDS             complement(11265..12212)
                     /locus_tag="U4A29_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEO0680829.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12390..13193)
                     /locus_tag="U4A29_001705"
     CDS             complement(12390..13193)
                     /locus_tag="U4A29_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEO0680830.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            13339..14253
                     /locus_tag="U4A29_001706"
     CDS             13339..14253
                     /locus_tag="U4A29_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO0680831.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            14301..14771
                     /locus_tag="U4A29_001707"
     CDS             14301..14771
                     /locus_tag="U4A29_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO0680832.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            14856..15464
                     /locus_tag="U4A29_001708"
     CDS             14856..15464
                     /locus_tag="U4A29_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO0680833.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            15598..15798
                     /locus_tag="U4A29_001709"
     CDS             15598..15798
                     /locus_tag="U4A29_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO0680834.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         5534 a         2408 c         3223 g         5223 t
ORIGIN      
        1 ggaatttatt ctaactattc attgattgtc acaagtgtgc aaaatttatg taagcaaatt
       61 actaattcta tcacggcaag tgtaggaaat tttgctgtat caaataaaaa aaaggattca
      121 tttgagctat ttaaaaaaca tttttttatc aaccactcac taatcttttt ttcttctata
      181 ttgttattga ctttaatgaa tcccttcatt agcttgtggg taggagccaa atacatgcta
      241 cctatgtcaa cgactatttt aattgtttta aattatagtg tacaggttta tagaaataca
      301 tccttcgttt ttatagagtc atttggttta tattggtctc aacggaaaaa gcctataatt
      361 gaagcaggtg taaacttgtt attatctttg cttttgttaa tagtttttga tatgggaatc
      421 aatggagtac ttatagggac tatcggatcg tctctaggat ttgtgatttg gtatgaagca
      481 tttatcatat ataaaacggt atttgagaaa cgttttattc atttcttttt aactttctgg
      541 cgaagtttta tagaattaat aatttcggca atagtcgttt ttctattaaa tgaatattta
      601 gcaaatggag gcagtacgtt ccttcaattt ataactaaaa ccttaataac tatagtaaca
      661 acatcgatat tatatttgtt attatatttt agaagagaag aatttacatt tatcaaaaaa
      721 attcttttta ggtatatttt aaaaaaatag ctttctataa aggagtgttt tatttgaaaa
      781 tagctatact atctccagct ttctcaatgg gaggagctag tatcgtggcc tcaaaaattg
      841 caaaaggatt aatagatagt ggccatgaag ttttttttgt ttcttactta gaaagtgaaa
      901 aaccacaagt agaatctaag tattatgatt tatacgaaaa aaaaattttt ttatttaaaa
      961 atttttctcg atttcataag atattagagt atagattgtt tggaggcttt gttcctagta
     1021 gatatataaa aaaagaaaaa gaacaactaa aaaaagttct aaagaaagaa aaacctgatg
     1081 tcatcatatt caatactttt atacctagtg tactattctc cgaattccta aagtatgaat
     1141 ttccaacagt gaaattaatt acttggatgc actctgatcc tgattacagc ttaaatcata
     1201 ttgctaaatt ttataaaaaa gaatatagaa aaagttttga acgcgtggat tccattgttt
     1261 gtctttcgca agatacaaaa gaaaaacttt cattatacaa taaccatatt aaagttattt
     1321 ataacccatt gacattaagc ggctctagtt taataagtaa ccttgataaa aaaattattt
     1381 catttactgc cagatacgat ataaatatta aagggtttga ttatttatgt gaagtagcta
     1441 aaaacttacc tgttggatgg gctataagga ttgctggaga tggtacagaa gaagaacgta
     1501 aagagctgaa aagattaatt gagaaaaatg gtgtgttaga taaaattatt tgcgcaggtg
     1561 cattatttgg agatgaatta atcaatcatt atatagaaag ttctattttc atatctactt
     1621 ctagaagtga gggactacca ttagtgttga tagaagcaat gacttatgga ttacctgtaa
     1681 taagttttga tcatggtggt gctaaggaaa tattacaaga tggaaattat ggaattttaa
     1741 ttaagaattt tgacgtacaa gatatgaata atgagataga aaaattgtct aataatcgtt
     1801 tacttatgga aaaatatcaa aaattctctt tagaaagagc taaggatttt aatgaaaaaa
     1861 aaataattgg tgaatggcaa acattgttaa atgaattagg aaatgaggat gttagaaatt
     1921 gaggacttcg attaaaaatt ttaatttatg gaaagaatgg tatctatata tattttttat
     1981 agggatgatt attcgtgcaa taaattttta tagtttatta ccgtccactt ttgatagtat
     2041 tttttttaaa cttataggaa tttcaggagt tttgctatta gggatagatt tcataattaa
     2101 atttaataaa agaaaagtaa cttataacat tttattgata gcatatatgg tgattttagt
     2161 aataacgagt tttgttcata aagaatatgg tctaggagaa aacttaaaga ctataatgtg
     2221 ggcaatctta cagtattttt tgatttatca gtttgctttt gagaatagcg ataataaacg
     2281 attttttaaa aggataagtt ggggatttat ttatagttgg tttgttttag tatcaatgtc
     2341 aatggttctt ttctttggta aattcggcta tgagaaatat tataatgcta gacataggat
     2401 cagaattggt tttttagaat caagactgtt tgggacgttt tctgatatta acaatgctgc
     2461 tatagcatct ttagttgtta ttgtcctaat cttattttat ctttataata aaaaagtgac
     2521 aggattaaat aaaatcattt ttttaattaa tcttccatta caatttattt atatagtatt
     2581 aagtgggtca cgatctacct atgtggtaag tataataata gttgccatat catcttttat
     2641 tgttgtgtta aaacgaaatg gaacaaaaaa agatttaaaa aactatatcc ttgctatagt
     2701 atcttctatt ggttctgttc tgattttatt cgctttgttt aatgcaacta agatactttt
     2761 tcaagaatta ttacctcatt taaaagaaat aaattttttt aattttacag agcgtcataa
     2821 agaattaatg aataatagtc aacatattaa taatactcaa acacctgata atactcttgt
     2881 taggaaagac gtagctgaaa atacagatat ttctaatgct agaattacta tatggaaaag
     2941 tgcatgggaa atatttaaaa caagttggct tatcggagtc tctccgaaaa atgtagtatc
     3001 ttatgcacag tcaattattc cagatggata tattgcgaaa gtaggaattg ccattcataa
     3061 tgcatacttg aatgtattaa catcgacagg tattctagga ataattcctt tcatggcttt
     3121 tttaataaag agttttgtgc tgaatgttta tcgaatcatc tataataaat gcattggtaa
     3181 agattatcat tttgtttact gtttagttat tttatcttat gcactatacg cagctcttaa
     3241 caatgaatta gtctatgaaa acactgtagg aacgtttgtt ttttggctat ttttaggaag
     3301 agttaatagc aaaaataatt ctagctattt ttagtaataa attttatctt gaaaagagga
     3361 gaaaaaatgt tgaattactt gattaccggt ggcgccggct tcattggctc gacgctcgcg
     3421 aattatctag gaaaagaaaa caatgtagtt gtgatcgatg atttatcgat ggggaaaaaa
     3481 gagaatctgg atgaatcgaa gcacatcacg tttattgaag gggatgtcgc tgacaaaaat
     3541 ttgatggaaa aggtcatgaa agaataccat ttcgattata ttttccatct agctgctgta
     3601 gctagtgtgg cagattctgt agaacgtcct ctagaaactc atcgtgtaaa ttttgacagt
     3661 gcgcttttat tgcttgaatt ggtacgaaaa tatcaatctg gtttaaaacg attggtattt
     3721 agttcatcag ctgcagttta tggcgatgaa ccaacgttgc caaaaaaaga agaatcggtc
     3781 atccgtcctt taacaccata tgccatcgat aagtttgcag cagaacaata tgttttggat
     3841 tactgtcatt tatatgatgt tccgactagt gctgtccgat tttttaacgt ttatggtcca
     3901 aatcaaaatc ctaactctcc atatagcgga gttatttcga ttctagtgga ccgatacaaa
     3961 aaacaattgg caggagaaaa aacagaattt actttatttg gtgatggtag ccaatctaga
     4021 gacttcgttt atatagaaga tgttatacaa gcacttttac ttgttgctaa ggaagaaaaa
     4081 gctttaggac agcaattcaa tgtcggtaca ggaaaaagca ctacgctact tgaattgatt
     4141 cattcaattg atcaaatctt aggaacagag ctagcgctta aatatgaagc ggagcgttca
     4201 ggggatattc gtgattcttt agcggatatt tcaaagatcc gttctttagg ttatcaaccc
     4261 aaatttgata tattgaacgg aatggaacgt tatttaaaga cagaaatcga ttgaaacaaa
     4321 aaaccagcat cctgctggtt tttttgtctc gacttagctt tttaaattaa caccttccat
     4381 cagttcgtaa atctgatttc ttaaatcagt ggccggcaat ttgatgtttt tatcagattg
     4441 gatattactt tccatgacga ttgcatcttt tgtgtctttt ttcatcgccg ctactgcatg
     4501 gaatcgattg atatttccct gagcaaggat ataatcattt ccactgtaaa caccgccgct
     4561 gtaaggatgt ttatcaccat tattttgcca caatgagaaa agtagttctt ttggtataat
     4621 atcgccagac agaacactgg taaagaattt gttcaagtca taaacagaca tacttatatt
     4681 tcctgtgcct aattcatttc ggatgtctgt ttcaggctcg accaatgctt ttgaataatc
     4741 gtcagttgca gacatttggt aagatacagc atgctgaggg gaatttttta cattttcata
     4801 aaaatctgta tgttctagat ggagtggccg aatgatttct ttttttaaaa gagtttcata
     4861 aggctgattc attagtttga tcaatacccc tgtaagcaat gtataattta atggttcata
     4921 atgatacgta tcataaccag taaatttcaa atgatgaagg gcaaactgaa tgatctcact
     4981 atctgacata ggtttttttg gagaagcggt tcgatgaaga ccagagcgca tgtaaagcat
     5041 atttttgatt gagatttgct gacttccagg aatctgagga taaaattcag atagctttgt
     5101 gtcaaaagtt atcttgccag attgaatttg ttttaggata agtaaagcag tcatcgcttt
     5161 ttggatcgaa ccgatttgat agtaaagaga aggagaattc gattctttta tgcgtagatt
     5221 tgcgtaccca tatccttttt ctaatatcac tcgattattt ttgcgtacat aaatcgaacc
     5281 gatataatct gatttttgca gtaattgatc aattttgctg gcaagcatct gctcattaga
     5341 tgttaaattg gaagcaagtt gtgcgggttc cgttttataa atttgtggtt gaggttgttt
     5401 tgttaattgc catcctgtaa tgatgcctgt tgaaaagaag ctgacagcca gcaacaaaac
     5461 tgcgagaaaa gaaagagaga tctgctgttt tttttgatgt tttccttttg ccatattaaa
     5521 tccttcctat ccatttttaa ctatttttca ttgtaatcct caaaaagagc atttgccact
     5581 tacaattttg acataatatt tggtaaaata attcacggca gtagaaattt catgtataat
     5641 aaacagagtt acgaagatga tagtaaggag gagttgtttt gtcaggaaac aaacaacacc
     5701 caagcaaaga caaaaaagaa gccagtaagg cagctacagc agatataatc gacaagcgga
     5761 gaaatctgcg taaaaaagaa gataagatcg tgaggaagat cattttagtg atcgctctta
     5821 cattattgat tattggcgga tttctcgggt ttaccgtata tcgttatgta gatagcgggt
     5881 tgaagccttt agataaatcg gacgaccaat tagtccaagt ggaaatccca agcggctctt
     5941 ctaataagca gattggcgaa attttggaga aagacaatat tattaaaagt gggattgttt
     6001 tcaattatta tacaaaattt aaaaacttaa ctggctttca agctggttat tatcagttag
     6061 cgcctaatat gactcttgat gagattggca agcagttgca agaaggcggc acatctgagc
     6121 caacaaaagt tgcagatggg aaaattgcca ttccagaagg ctacgacatc gatcagattg
     6181 ctgagcgggt agcgaaggta actggtaaag acaagaaaga atttttagat ttagtaaatg
     6241 atgagacttt ctttaacgag ttgtatcaaa aattcccaga attgttggaa agtgcctcta
     6301 aagctgagaa cacaaaatac cgtttggaag gctacttatt cccagctact tatgattact
     6361 acacgagtac ttcgcttaag gatcttgtga ttgagatggt gaacaaaaca aatacggtca
     6421 tgcaaaacta ttattcagct atcaaacaga aaaatttgac tgtacaagaa gttttaactt
     6481 tggcttctct agttgaaaaa gaaggcgtaa aagaaaatga tcgtaaaaat attgcacaag
     6541 tcttctttaa ccgaatcaag gcaaatatgc cacttcagtc agatatctct gttttatatg
     6601 cattaggaga acataaagaa ttggtgacgt atgaagatac agctgtagat tcaccataca
     6661 atttgtatac caataccggc tatgggcctg gtccttttga caatccaagc gaagaggcaa
     6721 tcaaggcagt attagaacca gcagaaaatg actattatta tttcgtagca gatacgagca
     6781 ctggaaatgt ttatttcgct aaaacgtatg aagaacatat ggaactagtg caaaaatatg
     6841 taaataattc ttagaaaaac aggggctagt cattgatcat ttttagaaaa acatttacat
     6901 tttaagaaaa gcgtggtaat cttttttgga ctatattcct caaaagatta ccttttgcgg
     6961 tattttatcg aaaaaaggag acgttacaca tggtagagaa agtttatcca atgactctag
     7021 aaggtaaaga aaaattagaa aaagaattag aagaactaaa aacagttaaa cgcggagaaa
     7081 tcattgaacg aatcaaaatc gctcgcggtt tcggagatct ttccgaaaac tccgaatacg
     7141 aatcagctaa agatgaacaa gcatttgttg aaggaagaat cacaacaatt gaaaatatga
     7201 tccgttttgc acaaatcatc gataatgacg gcgtagatgc agatgaggta tcgatcggta
     7261 aaacagttac gttcatcgag ttgcctgatg gagatgaaga agagtacacg attgtcggaa
     7321 gtgctgaagc agacccgttt tctggaaaga tttccaatga ttctcctatc gcgcaagcat
     7381 tgattggcaa acgtaaaaat gatgaagtaa ccattgctac accaggtggc gatatgttag
     7441 ttaaaatcgt caaagtagaa caagcgtagt ctgactgaaa gatctagaga gttcatctta
     7501 ctgatgacgc actctagatc ttttctcttt gagtattgca ggtttcactg aacgatctta
     7561 catctaaagt cctatagcca acagtcaaaa aaataggtat gatgaaatga gaatgtgtca
     7621 ggagggagaa aaagcgatga atagttcttg gcgttttttt gtggtagtcg aagcactgct
     7681 gctgttattt gctgtttggc aaatcgtaaa caatactgga cttttgcttt tagtaatatt
     7741 cggtattttc aatatatatc tagctatgcg caaatatcct cgtactaaat ttcaaaattt
     7801 tcaattgatc ttagggagct tggtcatttt ttttagctta gtcaacagtc ctgctttatg
     7861 gatgatggtt gtattggccg ttttatttat cgggctgaaa ggtgtagaga tttcaggaat
     7921 agatttgaca aaaaatgctt tttggcgaaa aaaacagatc atgatggtcc aaacagagca
     7981 attaaaaaca cataataatg agcgtaaaag acagcaatta tttggtaacc aacgtattgg
     8041 aaacgacgtc tatgaatggg atgatatcaa cattgccatc atttctggtg atactatcat
     8101 cgatttgggc aatacacttt tgccaaaaga tgataatatt gtgattgtaa gaaaagggat
     8161 aggcagaaca cggatcttag tacccctggg agtggctatt cgtttagagc atgcgacatt
     8221 agtaggaaat gtcttgtttg aagaagagca gttttcctta aaaaacgaac agatcaaaat
     8281 ctgcagcagt gattatgatg aaaatcctcg cagattgaag atcattacca atactttggt
     8341 tggagatgtc gaggtgatac gaatatgatg ggaagaatat ccaaagcaat gctagctgtt
     8401 tattcgggga ttgcggcttt ccttattatt ctattttcac ttttcactta tttttatgcc
     8461 agtaaccaaa gtcattggtg gggagaattg ctgcgtgcgc gtttattata cgttccgctt
     8521 atcttccatt tgatggcaat ttcattaggt gtaggattga tcgtatttct attgttatca
     8581 cttattcaaa aggcaaaata cgggaaaatc gaagaaaagc ttcgtgcact ttcttctggc
     8641 aattatgaat ccaaactttt gcatcagccg atcccaagcg cgtcggatga tctgtacatc
     8701 aaagatattg atatggaaat tactaaaata aaagaaaaaa tgatcgaagt atccagtgaa
     8761 ttacagatta tgactagccg tcctcaatat gtagatggac aaaccaaaga agagatttta
     8821 aaattagaaa ggcatcgatt agctcgtgag cttcatgatt cagtttcaca gcaattattt
     8881 gcagcgatga tgatgatgtc ggcattgacg gagcaagcag aaaaaagtga gacaccagaa
     8941 atgttccgta agcagttgaa aatggtggca gagatcatca acgcttccca gtctgagatg
     9001 cgtgcgctgc ttcttcatct gcgtccagtc aatttagaag aaaaaagttt aaaacaaggc
     9061 attgagcagt tattgaagga attgcagaat aaaatccaga tttcgctaaa atgggaggta
     9121 gaagatgtaa aattaactag ttccattgag gatcacttgt tccgaatcgt tcaagaattg
     9181 ttatcaaaca cattaagaca tgctaaggct aatgaattag aagtctattt gcataaaata
     9241 gataacaatc ttttattacg tatcattgac gacgggacag gatttaatat gaatgaaacc
     9301 aaaacgggaa gttatggatt gaacaatatc aaagaacgag tagctggtat tggtggaaca
     9361 gtaaaaatca ttagttttaa agggcaaggt acgagcgtgg agaccaaggt ccctttgatg
     9421 aaggaggcat agataaagat gataaaagtt ttattagtag atgaccatga aatggtacgc
     9481 ttaggcgtct cttcttattt atctatccaa gaagacattg aagtgattgg agaagctgaa
     9541 aatggacgtc aaggctatga aaaggcgatg gcacttcgac cggatgtcat tttaatggat
     9601 ctagtcatgg aagaaatgga tggcatcgaa tcaacaaaag caatcttaaa agattggcca
     9661 aaagccaaaa tcattatcgt cacaagtttt attgatgatg aaaaagttta tcctgcaatt
     9721 gaagcaggag cagcaggata tttgttgaag acgtctacag cacatgaaat agctgatgct
     9781 ataagggcta cgcaacgcgg cgagcgagta ctagaacctg aagtgacaac aaaaatgatg
     9841 gaaaaaatga gtcggcgaaa tgatccagta ttgcatgaag aattaacgaa tcgagaaaat
     9901 gaaattttga tgctgatttc tgaagggaaa agtaatcagg aaatcgcgga tgaattgttt
     9961 atcacactga aaacagtcaa aacgcatgtg tcgaatatat tagctaagtt agaggtagaa
    10021 gaccgaactc aagcagctat ctatgcgttt aaacatggtt tggtgaaata aaaacgatga
    10081 taaagaggat aatcaaaagc tgagaaaata actattattt ttcttggctt ttttacaata
    10141 atgatgcttg cttttgtttt tggaaaggct ttgttatact gagaaaagaa gatactaaag
    10201 gaggaaaatg gtgaaacaaa attttgcaat tgtaggcctt ggacgatttg gcggaagcat
    10261 ctgccgtaca ctgatcgaag ctggacaaga agtactggct attgacagta atgaagatcg
    10321 agtcaatgaa tatatgaata tagccaccca tgctgttgtg gcaaatgcac aggatgagat
    10381 gacacttcgt tcattaggta taagaaattt cgatcatgta attgtcgcta ttggagaaga
    10441 tatccaagcc agcatcttag ttactctgat ggtaaaggaa atgggcgtac cgaatatttt
    10501 agcaaaagcc caaaatgagt accatgcccg tgtactagag aagatcggag cagatcgtgt
    10561 cgtacaccct gagcgagaca tggggatacg gattgcacat aacttagtat caaagaatat
    10621 tctagactac ttagagctgt cagataaata ttctttagca gaaatccgag tctccaatcc
    10681 taaatttttc aacaaaacat tagctgaatt aaatttcagg caacgttttg acttaacagt
    10741 agtcgccata cgaagagtag atcaaacggt tatcgcttct cctgcagcag atgaatatgt
    10801 tcgtgaaaat gataatctac tggttattgg agagacaggc gatgtggata ttctagacga
    10861 taagatgaat caatagaaag aaaagagaaa gtaggttgtt gggatgaaat taacggtaac
    10921 gccaaaagca caaaagtggt ttgactcaga aattgatctg ccagaaactt acggaatacg
    10981 tttttttgga aaagtctacg gcaaaacaga ggttcatgat ggtttttcga ttggaatgtc
    11041 agttgaacag ccagaacatc ctgtaaagaa agtggaaatg gatgggacac ttttttttat
    11101 cgaggaagca gatgaatggt tctttaaagg atatgattta cttgttgatt atgatcccaa
    11161 cctggaggag ccaacctatc actttaaaaa acaataaaaa aagagagggt gacacaattt
    11221 ttgtcacctt ctcttttttc ttttgaaaat attgttttca gctattaata tcctgcagac
    11281 aattttactt gattggtttg aagctcatgg gtggcaaagt agcttttcag attggttaaa
    11341 aaaatagcca ttaatttctt ttgaaaatca ggtgtcaaac ctgcaatgtg ggaagtaatc
    11401 agtacattgt ccattttcca aagaggatga tcttgtggaa gaggctcttc ctcaaaaacg
    11461 tctaatgctg caaaggaaag ttgctggtta tttaaagcct gcaccagatc ttgtgtctta
    11521 acagaagcac cacggccaac attgatgaat acagattttg gatcaaagtg ttcaaacata
    11581 tgctgatcaa agagcccagt tgtttcctcc gttagaggta agatattgac tacgaaatct
    11641 acttcaggaa gaacttcctt tagttgattt aaagaaaaag tcttttccat tccttctgct
    11701 ggatgaccgg atgtattgat tccatagcag ttcatgtcta atgctttgca tttttttgca
    11761 atcatttttc caatatggcc agttccaatg attagtattt ttttcccttg gatagattgg
    11821 taatggaccg atgaacctaa ccatttcatg tcactctgtg ccttttgtgc ttgaaaaaga
    11881 ccacgagaat acccaaggat gattcctaag acatgctccg taatcgattc actgtggatg
    11941 ccactaccat ttgaaagtaa aatatgtttc tttgaaaaag tctcaagggg tagctgatca
    12001 acaccagctg agatagattg gacccatttc aaggtagaag aggctaatag tttttcttgg
    12061 tagtctgagt tccagcccaa actgatctct acattttgta gatcctctgg ttgtaattct
    12121 gttttaaaga tataaccagg cgctatagta tggatttctt cgataaattc atcacgaaat
    12181 ggacgatctg cataaatgat tctttctgac atagttaatt tcccgccttt ctttactcta
    12241 agtgtagcgc atacaagaag aactttgaag cgccttgctt gttttttgag aaagaaatat
    12301 ttttcaaata aaaaaggggc tgtgacaaaa aaatgtcaca acctctaaaa gggtctattg
    12361 aagtagaaat aacgagttgt ctttatttct taattgatcg atatttttgc ccatcctttg
    12421 acagcttgag caaaaataaa gattcctgca aaagcaatga aggcgctagt gtcaaaagaa
    12481 ggaagaaaga ccatcaagat tgttgcaatt atagtaggca atgaaattga atagatcaat
    12541 gtttttaaac aatcaagaaa agtagctttt gttagtcgaa agcgtgcata gagaaaagca
    12601 gcaatgttgg taaatagcaa agtgatcagc agattcagaa aactaggata aatagaaaat
    12661 ataaatgtca ggacttttat ccaaagtggt atagatgctt cacttaaaaa agaacggagc
    12721 tgcttacctg ttaagttttt taaagattca ttggtgtagg gaagttctaa ttggttattg
    12781 ttcaacaaag cagaagtcgt gcctgtatta ggaagagcta tgataagttt gttttttaac
    12841 atcccgacac taacaaaatt ccccatcaaa tcagtagaga tgtcctgttc tgttcgtttt
    12901 ccctctggat caaatgtgaa gataatggag tttgtttggt aaatgaatcc ttctttttct
    12961 tcggtttgaa gctgcccgtt ttggattgta aaatcaggaa tgcgagtagc aatcttttgc
    13021 ccatccaatt gaatgttgtt taagacttga aaaacttgaa aagagattgg caaagccata
    13081 attatactca aaactaaaag atagacaatg gctttccaaa atgggacatt tctcgcctcc
    13141 tttaggtctt tgaatcgtat aaaggaactg ataattaatt gttttgttgt cataagtact
    13201 cctcatctag tagtgtcatc acttgcaatt ctagcgaagt atcaagtgaa atacaagtgc
    13261 tttaaggaaa cagctgaatg attggtgaaa tatcaaaaaa atgttattct tgtctaaaga
    13321 ctatccaata taaataaaat ggaggaagga atagtgccta cacagccaca gagatcatca
    13381 aaaaaactat ttggagtagt ggttttgaat gcggtgatca ctttatcaga atttattgga
    13441 gggattatgt cagggagttt gtctcttgtt tcagatgctt tccataacct tagtgataca
    13501 ttggcaatta ttttttctta cggggcacaa aaaatggccc gaaaagaagc aaataaaaaa
    13561 cgtacgtatg gctaccagcg tttagaaata ttgagtgctt tcatcaactc attcatattg
    13621 atcatattat ccttgttttt aaccgttgaa gcattcaagc ggttcacttc accagaaaag
    13681 ataaatagtc atttgatgct aacagtagca gttattggat tgctcgcgaa tttgttctca
    13741 gtattattat tacgacaaga agcagatgaa agtttgaata taaaatctag ttatttgcat
    13801 ttgttaagtg atacactgtc ttcggtaagt gtgattatcg gagcaatttt gatccgtttt
    13861 ttcgatatct actggatcga tccagttatt acactgatca tttcgatata tatattgata
    13921 gaagcgataa tggtgatcaa aaaagctgcc gctattttga tacaaagtgc acctgctatc
    13981 gactatgaaa aaatggaaca agaaattgaa gcaatcgatg gggtaaagga tgttcatcat
    14041 gtccatattt ggcagtatag cgaaaagata attattttcg acggacatat tgattttgaa
    14101 gatcagctac taagcgagat tgaaaagata tatcctcgta taatttcttt attaaaatta
    14161 aaatatggta ttacacatgt gacgatccaa gcagagacaa aggcaaagga tcaaaaaaaa
    14221 ttaatttttt taaataaaga tgaaagttat tgaaatatag agtatgataa ataagaattt
    14281 ttaataaggt aggttggctc atgaagcagt atcgacagtt aaaaggatat ttagaagcaa
    14341 aaggatattg ggagatacta gtctatctct tttttggtgg tttggctacg attgtaaatt
    14401 ttgttagttt tgctctcgca cgccaatatt ttgatttaag tatggcctta tccaattcta
    14461 tttcgtggtt ttgttctgta ttgtttgctt ttgtaacgaa caaactttgg gtattccatt
    14521 caaaatcacc gaattttaca catgcactta ttgaatgcgg taagttcttc ttctaccgta
    14581 tcctttctta cggattggac atgggggcaa tggtattgtt gatcaatgtt atgaacagca
    14641 atgagtatgt ggcgaaaatc atcactcaaa ttattgttat ccttgcgaac tatattttca
    14701 gcaagttgtt tatttttaaa gaaactgagg tttttgaaga agaagtaggt caaagtgaaa
    14761 aagacgacta ggcataacaa ttgaagaatt tccttctctt tgttactctt tgtaatgtag
    14821 acattattaa gaaaacattg aggaggaatt tttttatgac ttacacatta ccagatctac
    14881 cttatgcata tgacgcattg gaaccttata ttgacgaaga aacgatgcat ctgcatcatg
    14941 ataagcatca caatacttat gtgacgaatt taaattcagc aattgagaaa tacccagaat
    15001 taggcgaaaa aacaatagaa gaattattat ctgatatgga cgctattcca acagatatca
    15061 agacagctgt acgtaacaat ggtggcggac atgctaacca ttcatttttc tgggaaatca
    15121 tggcaccaaa tgctggtggc gaacctacag gagaaataaa agaagcgatt aatgaagctt
    15181 ttggtgattt ttcttctttt aaagaagaat tcaaaaaagc agccgctgga cgatttggtt
    15241 ctggatgggc ttggcttgta atggaaaatg gaaaattagc tattacctct actgcaaatc
    15301 aagattctcc attgatggaa ggaaagacac caattctagg tttggacgtt tgggaacatg
    15361 cttattattt gaaatataaa aatgtccgac cagattatat tgcagcattc tggaatgtta
    15421 ttaattggga tgaagtaaac aaacgtttcg aagcagcaaa ataaccattt cctccgtcat
    15481 atcagtctta aaaataattt ggaaaataaa agagcgaaag cttgctagcg ttttctaaat
    15541 tgggtataat gttcttgcaa ggttacttgc aagaactttt ttggaggata tacatttatg
    15601 gaacacggaa cggttaaatg gttcaacaat gaaaaaggat ttgggtttat tacagtagac
    15661 ggcggagatg acgtatttgt tcatttctca gcaattcaag gagacggctt caagagccta
    15721 gaagaaggtc aagaagtaga attcacgatt gttgaaggtg cacgcggacc tcaagctgct
    15781 gaagtgacta aattataata atttgttgaa taaagacacg acttcttaga gagtcgtgtc
    15841 tttttgtcta tttatgaagt ttttagattt tttggctaag taaatgcatt tgttcacttg
    15901 acatgtccta actaattatc aagcaaggaa cattttcttc gttagtaatg gattttaaag
    15961 aaatttcagt ttttggatgt tgaataaaag accatttgtg aaaccactag tatacatact
    16021 cattaccatg tcaattctat tactttaaaa gaaaatcgtt ttactttgaa gaggagcata
    16081 tctatccatt aaaagatgat ggtaatgcaa tcactagttc gagtcatttt aagagccatg
    16141 cagtgagctg gaaataaaac gaatagaaga tgggtttaac aaaaggacca gtcataggaa
    16201 gaaattgtcg tgaaatcgaa tgataatctg aacggtaggc aaagactgtt aaaaagatgg
    16261 gaaaggtatg tgcgatcatt ttactgcacg attatttgga aaataccttg ttgttcgctt
    16321 tttctggaaa aaatacataa aaaaagataa aacgattgcg aactgcgtgt attcggtctt
    16381 tcgatctt
//
LOCUS       DASOUD010000057        16350 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.057, whole genome shotgun sequence.
ACCESSION   DASOUD010000057 DASOUD010000000
VERSION     DASOUD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.057"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(152..550)
                     /locus_tag="U4A29_001710"
     CDS             complement(152..550)
                     /locus_tag="U4A29_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEO0680835.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="U4A29_001711"
     CDS             complement(547..1320)
                     /locus_tag="U4A29_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEO0680836.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="U4A29_001712"
     CDS             complement(1317..2000)
                     /locus_tag="U4A29_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEO0680837.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="U4A29_001713"
     CDS             complement(2167..3054)
                     /locus_tag="U4A29_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO0680838.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="U4A29_001714"
     CDS             complement(3051..3920)
                     /locus_tag="U4A29_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0680839.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="U4A29_001715"
     CDS             complement(4057..5343)
                     /locus_tag="U4A29_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO0680840.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="U4A29_001716"
     CDS             5616..6194
                     /locus_tag="U4A29_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO0680841.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="U4A29_001717"
     CDS             6194..7123
                     /locus_tag="U4A29_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0680842.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="U4A29_001718"
     CDS             7120..8856
                     /locus_tag="U4A29_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO0680843.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="U4A29_001719"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="U4A29_001719"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEO0680844.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="U4A29_001720"
     CDS             complement(9752..10261)
                     /locus_tag="U4A29_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO0680845.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="U4A29_001721"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="U4A29_001721"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO0680846.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="U4A29_001722"
     CDS             complement(11562..12653)
                     /locus_tag="U4A29_001722"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEO0680847.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="U4A29_001723"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="U4A29_001723"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO0680848.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="U4A29_001724"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="U4A29_001724"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO0680849.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="U4A29_001725"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="U4A29_001725"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO0680850.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4714 a         3616 c         2908 g         5112 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag
//
LOCUS       DASOUD010000058        16203 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.058, whole genome shotgun sequence.
ACCESSION   DASOUD010000058 DASOUD010000000
VERSION     DASOUD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.058"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..894
                     /locus_tag="U4A29_001726"
     CDS             226..894
                     /locus_tag="U4A29_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEO0680851.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="U4A29_001727"
     CDS             complement(1094..1480)
                     /locus_tag="U4A29_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680852.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="U4A29_001728"
     CDS             complement(1452..2834)
                     /locus_tag="U4A29_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEO0680853.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="U4A29_001729"
     CDS             complement(2990..4177)
                     /locus_tag="U4A29_001729"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEO0680854.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="U4A29_001730"
     CDS             complement(4184..5215)
                     /locus_tag="U4A29_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEO0680855.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="U4A29_001731"
     CDS             complement(5231..6685)
                     /locus_tag="U4A29_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO0680856.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="U4A29_001732"
     CDS             6860..7765
                     /locus_tag="U4A29_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO0680857.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="U4A29_001733"
     CDS             7856..8533
                     /locus_tag="U4A29_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO0680858.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="U4A29_001734"
     CDS             8530..8700
                     /locus_tag="U4A29_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680859.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="U4A29_001735"
     CDS             8814..9710
                     /locus_tag="U4A29_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO0680860.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="U4A29_001736"
     CDS             complement(9765..11135)
                     /locus_tag="U4A29_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0680861.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="U4A29_001737"
     CDS             11373..12119
                     /locus_tag="U4A29_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO0680862.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="U4A29_001738"
     CDS             12116..12955
                     /locus_tag="U4A29_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO0680863.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="U4A29_001739"
     CDS             12956..13906
                     /locus_tag="U4A29_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO0680864.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="U4A29_001740"
     CDS             14047..14799
                     /locus_tag="U4A29_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680865.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="U4A29_001741"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="U4A29_001741"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO0680866.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5259 a         3022 c         3249 g         4673 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa acatagaaaa acgattactt tgcttataca acaaggcttt tatggttttg
    16201 tat
//
LOCUS       DASOUD010000059        15860 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.059, whole genome shotgun sequence.
ACCESSION   DASOUD010000059 DASOUD010000000
VERSION     DASOUD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.059"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(12..482)
                     /locus_tag="U4A29_001742"
     CDS             complement(12..482)
                     /locus_tag="U4A29_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEO0680867.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="U4A29_001743"
     CDS             complement(641..1099)
                     /locus_tag="U4A29_001743"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO0680868.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="U4A29_001744"
     CDS             complement(1270..2415)
                     /locus_tag="U4A29_001744"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEO0680869.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="U4A29_001745"
     CDS             complement(2428..3345)
                     /locus_tag="U4A29_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEO0680870.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="U4A29_001746"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="U4A29_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO0680871.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="U4A29_001747"
     CDS             4437..4607
                     /locus_tag="U4A29_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680872.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="U4A29_001748"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="U4A29_001748"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEO0680873.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="U4A29_001749"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="U4A29_001749"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEO0680874.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="U4A29_001750"
     CDS             6871..7752
                     /locus_tag="U4A29_001750"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEO0680875.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="U4A29_001751"
     CDS             7749..8465
                     /locus_tag="U4A29_001751"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEO0680876.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="U4A29_001752"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="U4A29_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEO0680877.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="U4A29_001753"
     CDS             9182..10183
                     /locus_tag="U4A29_001753"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO0680878.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="U4A29_001754"
     CDS             10180..11541
                     /locus_tag="U4A29_001754"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO0680879.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="U4A29_001755"
     CDS             11501..12652
                     /locus_tag="U4A29_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO0680880.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="U4A29_001756"
     CDS             12627..13796
                     /locus_tag="U4A29_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO0680881.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="U4A29_001757"
     CDS             13971..15350
                     /locus_tag="U4A29_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO0680882.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..15860
                     /locus_tag="U4A29_001758"
                     /pseudo
     CDS             15631..15860
                     /locus_tag="U4A29_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5184 a         2800 c         3379 g         4497 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctaacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaagaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tacagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcatacag ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg gggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccgc aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc gaggaaaagt
//
LOCUS       DASOUD010000060        15825 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.060, whole genome shotgun sequence.
ACCESSION   DASOUD010000060 DASOUD010000000
VERSION     DASOUD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.060"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(55..1830)
                     /locus_tag="U4A29_001759"
     CDS             complement(55..1830)
                     /locus_tag="U4A29_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEO0680883.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
     gene            complement(2041..2943)
                     /locus_tag="U4A29_001760"
     CDS             complement(2041..2943)
                     /locus_tag="U4A29_001760"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEO0680884.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3222..5567
                     /locus_tag="U4A29_001761"
     CDS             3222..5567
                     /locus_tag="U4A29_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO0680885.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(5730..5996)
                     /locus_tag="U4A29_001762"
     CDS             complement(5730..5996)
                     /locus_tag="U4A29_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680886.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(5989..6312)
                     /locus_tag="U4A29_001763"
     CDS             complement(5989..6312)
                     /locus_tag="U4A29_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680887.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(6324..7580)
                     /locus_tag="U4A29_001764"
     CDS             complement(6324..7580)
                     /locus_tag="U4A29_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO0680888.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            complement(7577..7972)
                     /locus_tag="U4A29_001765"
     CDS             complement(7577..7972)
                     /locus_tag="U4A29_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680889.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            complement(8326..9327)
                     /gene="ccpA"
                     /locus_tag="U4A29_001766"
     CDS             complement(8326..9327)
                     /gene="ccpA"
                     /locus_tag="U4A29_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO0680890.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            9537..10640
                     /locus_tag="U4A29_001767"
     CDS             9537..10640
                     /locus_tag="U4A29_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO0680891.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(10703..11317)
                     /locus_tag="U4A29_001768"
     CDS             complement(10703..11317)
                     /locus_tag="U4A29_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO0680892.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(11310..11759)
                     /locus_tag="U4A29_001769"
     CDS             complement(11310..11759)
                     /locus_tag="U4A29_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO0680893.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            complement(11884..12822)
                     /gene="galU"
                     /locus_tag="U4A29_001770"
     CDS             complement(11884..12822)
                     /gene="galU"
                     /locus_tag="U4A29_001770"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO0680894.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            complement(12837..13862)
                     /locus_tag="U4A29_001771"
     CDS             complement(12837..13862)
                     /locus_tag="U4A29_001771"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO0680895.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(13906..14736)
                     /locus_tag="U4A29_001772"
     CDS             complement(13906..14736)
                     /locus_tag="U4A29_001772"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO0680896.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            complement(14751..15689)
                     /locus_tag="U4A29_001773"
     CDS             complement(14751..15689)
                     /locus_tag="U4A29_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO0680897.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
BASE COUNT         4456 a         3315 c         2866 g         5188 t
ORIGIN      
        1 caccgtttat tcggaaaaag gaactgcgac aagacttttg tcacagttcc ctttttattt
       61 ggaagataaa gcttttttca gattcaattt actaaaaact agcatgcttg ttactgcgat
      121 gaattgcgtt aatggcacgc ctgcgccagt gtagacactt aaaaaataat cctttttttc
      181 tggcgcataa gaacaaaacc acgagacacc gaaaagaaaa accaattgtg tcagtattaa
      241 tccgatgact acaatcaaag aggaactata ataggcacga tcagcttttg caaatacggt
      301 tcctagtatc ataagtaata tccctgctat tgaagcaatc agcacgggaa tggccagaac
      361 ttggtaaatt tttatcatga cattcgcgac ttttgctgtt tttgtcaact ggaaatcagg
      421 tgttctcgga tcactgctat cgatccaatt atccattagt ggaagattca ataattcttg
      481 tgcctgttcg acttgttctt gcgtcccaaa actataattc cccccttgat ggaaatcttt
      541 gtatcgccat gcataagcat atcctgattt cagaaaatca aatacgattg gcagatcatt
      601 catcgtcttt ccatcccctg atcccatcag atagaattct tttttctttt tgatagttcc
      661 cttttgatag gcttcttcta attcttgatt gacttttttc caaaaatcat tggtttcttt
      721 tgcattgttt tggtaataac cagattcgct taccacatct cgcaatgccc aaaaaatgat
      781 atctcctggt aattctttta cttttccgcc ttgggtccac gcatgagtcg tatacaattc
      841 gtcaatacga gtagaaacag tatgaaatgt aggagatgcg tctaaagctt tatttaatgc
      901 agcatgcgag acccatatct cactgttttc tttatttaag tttgtttcgt catctattcg
      961 gatcagctgt ttcatcaaac gtccaaattc gccactcgaa cggtcattga tggctgcaat
     1021 accatatgtt gtctcgtttt tatattcgat ccactgatag acgatggaga aaccgactag
     1081 agggagtaaa aacaatgaaa gtttcagcca ttcttgtttc gaaaggagaa atggtcgttc
     1141 ttttaacttc tttgcttctt tgaaagaaaa gtcttgtcct agcaagatct gaacgaaact
     1201 gatgcctaac gcgactacag taaaaggaag aagccagatg gaatcttcgc gaatgaacca
     1261 aaagaaagga aaagaaacag ctagccctaa aaaccacggc agcaattttt tgctggaatt
     1321 atatttgcgg taataaatag ctagtaaaca agcgataatg atctcaacag tcgggatcac
     1381 gagagcatta cgatatgttc gagtggaata ttcatgtgta aaactaatcg gagaaaacaa
     1441 aaaataaaaa tagatactta aaagaagata cttgttcttc acgattggtt taaacgctag
     1501 tgcaaccaat atactggcaa gaatactgaa catgccgaat aaaactgcat aagaaagatg
     1561 cagtttatgt gcaagtacta aaaacaagct gtaggaaatc ccttttgaaa gtgtcgtagt
     1621 cgtgtactcc cctagccatt taccatccaa aatattttga gcatagtgaa tcagcagctg
     1681 atcatcatag gtcgccgaca tatctgttcc atagattgct ttactatata aatacaaccg
     1741 aataattgta aataatacgg ctatgccaag aataaagtat gtatgtttct tttttagttg
     1801 gtcgatcaag gggtttaaaa tgttcagcat cttttcgtcc tttcaatcac gttttgctac
     1861 ttgtttgtga aacttttcgt tttttacttt tctatcagta taccctgttt gtctgaattt
     1921 aaaaagaggg ggcaaaagga gcatggcgaa aacgaatgca aaaagcactc ctttgtttct
     1981 taaacaaaaa aggacaaggc agactgccca atcctttttt gttgctgtca ggtagaaaac
     2041 ttaaagtgtg acttcttctg ctaataactc agggaacact tcgggatcga atgcttgcgc
     2101 tttgaacatg gaaatttctt ttccatatgg tgcgtaagct ttctttttat ctttaccgac
     2161 atatggggtt tctaatattt tcgaaatgtt gtcaaacatt ggatcatgga cgattttgtt
     2221 caatgtgtca aatccaattg ttccaaaacc gatattggca tgccgatctt tatgagaacc
     2281 ttgaggattt ttagaatcat tgatatgcag tactttaagc cgatctaatc cgatgacctt
     2341 atcaaactct tccattacac ctgaaaaatc ttcttttata ttatatccgg catcattcgt
     2401 atgacatgta tcaaaagtaa cggataattt ttcattcaat tttactccat cgatgatttg
     2461 cgctaattcc tcaaatgttc gaccgatttc cgtacctttc cctgccatcg tctccaaagc
     2521 gatctgtgcc gtctgttctt ttgtcaaaac ttcattcaac ccctcaataa ttctagcaat
     2581 cccagcttct gcccctgctc ctacatgagc gcctgggtga agggtgattt gagtagcacc
     2641 taaagcttcc gcacggacaa tctcttctct cagaaattgt acagcaaatg gaaacatttc
     2701 tggtttgatc gtatttccca aattaatgat ataaggcgca tgaacgacga tatttgttag
     2761 tccgttttct gccataaatg cttttccggc atcaatattc atctcttcga tagcttttct
     2821 tctagtattt tgaggagcgc ctgtatagat cataaaggtc gaagatccgt aactggctgc
     2881 ttcttctgcc gcgcctaaca gcattttctt tccgctcatg ctgacgtgtg agcctaataa
     2941 catggatgga ttcctccttg aaaggcaatt gtttcgaaca attcctgtat attgataaga
     3001 tgatgtacaa agaaaatagt ttgtttttct ttgccctgtt ctccatagta actgaaactt
     3061 tccctaaaga aaagcttaag aactctttca taaaaaagat catttcctca cgttttatct
     3121 cattcgaaaa agaaattcct gtcaaaaaca gccttgtctg tttttttgtc ctattatttg
     3181 ctataattag cgaaataaaa ccattagttg aggtgaccct tttgccacgc aaaaatactc
     3241 gaaaaaaaca gaatagaaga aaaaaagaaa aatggtttgt tcctaagatt atttttagag
     3301 tgtttcaatc attgactgtt tttattactg ttcttctttt gttgttcgca gctctaggta
     3361 tcggaatagg tgctggctac tttgcctatc ttgtcgaaga cacacaattg ccgactaaaa
     3421 aagaactgca gacagaacta ggaaacatta cggaaacgtc aaagattgtt tatgcagata
     3481 atacagagat atcaaaaatc cagacagatc tgatgcggac gactatttca tctgataaga
     3541 tttcgccttt attgaaaacc gcgattatca gtacagaaga tgaatacttc gataaacacc
     3601 aaggttatgt ccctaaagct gttttacgag cactagtttc agaagcgact ggcatcggct
     3661 cgtctggagg atccactttg acacagcaat tagtcaaaca acagatccta accgatgaaa
     3721 caacattcaa acgtaaagca aacgagatcg ttctttctgc acaagtagaa aaacactttt
     3781 caaaagatga gatcattact acgtatttga atgtctcacc atttggtcgg aataataagg
     3841 ggcaaaatat tgctggagtc caggaagctg ctagaggaat ctttggtgta gatgcaaagg
     3901 atgtgactct gccgcaagct gcttatatcg ctggtttgcc tcaaagccct atcacgtata
     3961 gtccatacac gaatacaggc gcgctaaaag aagatctttc tgccggtctt gccagaaaag
     4021 attttgtttt attcagtatg taccgtgaag gacaaattac gaaagaacaa tacgaagaag
     4081 cgaaagccta tgatcttaca aaagactttc tcccacaaca aatcgcggaa cagactgaca
     4141 gagaattctt gtattatacc gtgatgaacg aagcaacaag gattattgca caacaattgg
     4201 cagaaaaaga taatgctgat atgtcagatt catcggtcag tgaagcttat tatcaaaaag
     4261 cacagcaaac gatccaaaac aaaggatata cgatccattc gacgattgat aaagatatct
     4321 atgctgcatt gcaaaccggc gtagaaaatt atggctacat gttagatgat ggtgctggta
     4381 gccaagtgga gactggaaat gtattgatgg ataacagaac cggacgtatc tatggcttcg
     4441 tgggtggtcg gaattacagc cagaatcaaa ataaccacgc ctttgataca gaacgtcaag
     4501 ctggttcatc catcaaaccg gttcttgttt acggtcctgc aatcgatatg ggacttgtcg
     4561 gatcagaatc ccgtgtatca gattatgcaa cgacttggca agaaggaaaa aatgctggtg
     4621 agaaaatcgt caatgcgacc aatgaagggt cgaacacctt ccaaaccgtc cgtgagtcac
     4681 tggaatggtc caacaatatc ccagcttatc acttatatca agatgtgttg aattccggtg
     4741 gatcgaaaca atacgcttac gagcactact tggcgaagat gaactaccca gcaaatgata
     4801 attggggcgt tgaatcggct cctttaggga cagtagatgt aacgacgctt caacagacaa
     4861 atggattcca ggccttagca aatggtggag tctacgaaga aggctatatc attgattcca
     4921 tcacagataa tgcaggtaat gtgatttata aacatgaaag taattctgtc cgtatttatt
     4981 ccgaagcaac ggcatcgatc atgaatgaca tgatgcgtag tgtgatcaat gaaaaaatca
     5041 ctacaccatt caaagatgtc atctcaagct taaacggaaa tctaggaaat gcagattggg
     5101 tcggaaaaac tggttctacc aatgaatacc gagattcatg gctgatcgtc tcaactccat
     5161 ctatcactat cagtagttgg gcaggacacg atgacaatac tggaatggac agccaagcaa
     5221 gaatacgttc ggctaattat cttgccaatc taatcaatca agcttaccaa gcaaaccctg
     5281 agattttcgg cactgatgag aaatttgact tatcatccga tgtgatgaag aaaaaagtat
     5341 ctgggttcac aggacaaaca gctggaaaag ttactgtcaa taaaaaatct atccaaacgc
     5401 caggaaaaac agtggaatca ctttgggcaa aaaatggtcc gaagaaaagc gcctttaaat
     5461 tcggcgttgg cggtactgat gaaaactata cagattactg gaacaccgct tcagcttatg
     5521 ctagagcgaa tccagagacc aaagaatcag acgataaaga tgattgatct aatagattaa
     5581 tcagaattaa agggagctgc gacaaaaatc ttgtcacagc tccctttttc cgaataaatg
     5641 gtgttcaaaa gcttacatct acgatattaa gacaaattta tgtaaaaatg tgaaaaacca
     5701 ttttcacaaa atttttctat ataactttac taacgaaact gttttaattt cttttcttgt
     5761 ctttggaata attcggttag tttttctgca aattgccctt ttactgattc ttttaagtgt
     5821 gcatgtcggt tagaaaattc ttgagtagct tcttctgcag tcatttggtg gttttgatct
     5881 gtcaatcgtt tttctgccca agtttgctga gatatcccca taccaactga acgtacattt
     5941 gatcgatgta gatttttctc taattctttt agtaattgca ttaaaaattc attcataaga
     6001 tgatttctcc atagttattt gtctgatttt ttgacgatag gaacgttcta gttcgtcttg
     6061 ctgctgatag agttttcgtc gttctacttg tagggcttgt tgataatgct cctgataatt
     6121 cgaataactt cgtcttgctt gaaacagagg ctcattatct aaagctaagt tctttaatac
     6181 atgatgcagt cgctgctcct cttgctccat caatcgtaat ccttgattct taaagtggat
     6241 caattcatct tcttgagctt ctaattgtct tcgttcactt cgataatctc tttccaattc
     6301 ttctagtttc atctacttct cctttaattg aattgctgtg ctaattctcg atctcgtgcc
     6361 acgatttcat tgattttgtc ttggatctcc ttcgccaatg tatcaaaggc ctcccccata
     6421 cgtcttgcct tattgattcg tagcagatag acttctttcg cagaatcgac taagttctct
     6481 tttgtggcgc ccacactagc taatgcttct atgatttcct gttcagtcaa cgaaggggcg
     6541 acaacacgtg cttgccgcaa gccttcttgc cattttttct ctagctcaaa aattcctctt
     6601 tggttgatgc ggataacttc ttccatagcg cttttcattt ttaccgataa cttttccacc
     6661 actaacctag cttgtgtgtc atctaagtaa atttgttcat taactgtcag ccggccgcca
     6721 cttaatcgta gttttttacg tatgttgttc agttcttgca actgatagga aaacaactgg
     6781 gtcgcttggt tttctttctg ttgttttcga gctttaacat tgttttcatt ttcaggaata
     6841 atcagctttc cattttcatc aaacttccaa gaggttaaac tgtgggaagc aagcagattc
     6901 aagtatgctg tttccatact gatccgaact tcagcacctg tgccattacc accaaaatta
     6961 ccgataaaat cggcgtgatt tcggtaattg gtgagcatac cgggattttc ttccacccat
     7021 ttttttgctt ctggtgataa gagatcatac gggtccggcc cgttgaagcc gacatttttc
     7081 cattgtttct ctgctgctac atataatgct aagaatccac ctaatgaatg gccggtagta
     7141 gaaatagcag cttctccata ttcctttttt acttcattag caaatgcaat cgctgaattg
     7201 atttgccccg ctgttttcaa tccactaacg gataaaatct tgtttgttgt tgctttttca
     7261 gaaaaacttg ctcctggtct attcccatag agattcataa cacttcttgc atccgtttcc
     7321 aaatctttcc aatcgcttaa gttcgttcct gcaaaagcaa tcactacttg agaaatatcc
     7381 actttaccgt tcttgattgg cgctacagcc atcgcctgca tgccgtcgaa ggagtttttg
     7441 atttttagga tttggaattt tgtttctcca aagtttttaa tggcgccttc tttgtaactt
     7501 ttactatatt gaggatgttt tggatctagc caatatactt tattacttat tcctttatac
     7561 tgcttatcag taattgtcat actttttcct cactcccatc tgtatagata actataatct
     7621 catcaaccgt aatcccttct ttttgtactt cttcattttc aacaccatag ccatctatct
     7681 cattgctttc ttttgtaaag ctataatcaa aataaacggc ttttccttct gtattggtca
     7741 tcgttatcaa tgcttcatag attcctgtca tgtcgttttt ctcaaattgt tcgattttca
     7801 cttctttgat atccgcaaag gtatttttca gaactttgac ggactgtttt tctactgcga
     7861 gtaaatcttg ctgtttttgc gcttctcttt tgtccatgta aaattttcct ccgattccta
     7921 tagtaagaat ggtcaaaatg atgactatgg tctttcgtct tttcttaggc aaatagaagt
     7981 ctcctttcgg gttatgtatg atggattgtt tctcctttaa gataagtacg caatcgagcc
     8041 tggatttttt tggatttttt agagttactt ttctactttt ctactttctt ttttatcacg
     8101 ttttcatttt aaagtaattt tgatagtagt gaacaaaatg tagcatctac gcacagattg
     8161 tgtcgtatac gatggacgta aggtttgttt ccgccattct ctcttttttt taaagtcaca
     8221 aaaaaagggg ttatgacaaa attttgtcat aacccctttt ctcgtataaa ggttgttcca
     8281 gaagttgacc actttgggaa caacctttaa atgattgatc ttttattatt tagttgatcc
     8341 tttttcttcg attccataag gcaagataat cgttttttct tcgacttctt ctttattcat
     8401 cattttcgtc aataggcgca tggataccgc tcctaaatcg tataatggtt gtgtgatact
     8461 tgtcaaacga ggacgagcta cttctgtcag taaagaattg ttgcttgtga tgatttcgaa
     8521 ttcttctggt acttttacgc cgcggtctaa taaaccatcc aagataccaa tggctaattc
     8581 atcgtctgaa acatagacag ctgttgcacc gctattatgg atccggtcaa ctagagacag
     8641 accatcttta aagttgtatt gtgcctcaaa aatcaatcct tcgctatatg gtagattgtt
     8701 ttcgcttaat gcttctttat agccttttag acggttttga ccattgatag gatcaatcag
     8761 cgcaccgctg acaaaagcga tttttttatt gccgcttttt gctaatttat ttactgcatc
     8821 ttttgttgct gatacataat caatgtttac gctacctact tgttcatctg gatcaatgga
     8881 accagctagt acaacaggcg tttttgaacg tgaaaattct ccacggattt cgtctgtgat
     8941 atggtgaccc ataaagatca cgccgtccac ttgcttagcc aataaattat tcaatacatt
     9001 gacttctttt tgatcgttgc catctgagtt tgctaagatg atattgtatt tgtacattgt
     9061 tgccacatcg tcgataccac gtgctaaaga ggcaaagaac atattgctga catcaggaat
     9121 gattacccca actgttgttg ttttcttact tgccaagcca cgtgcgactg catttggacg
     9181 ataatccaag cgatcgataa cttccaagac tttttttctt gttgctggtt tgacgtttgg
     9241 attgccattt acgacacgtg aaacagtagc catcgaaaca tttgcctctc tcgctacatc
     9301 atagatagtg attgtttgct tttccatttg tgttctccta ttcatatttt atatgttttc
     9361 tgaaaatacg tcttacattt ctaaaatagg atatcaaata atctcaacaa ttgcaaccgt
     9421 tttatctctg ttttcatgaa aatttttact tttttcagtt ttaagaacgt tggtctttta
     9481 atcaatcagt ggtacaatta ttgaatacaa aattaattta gaaagtaggt tgccaaatga
     9541 ataacgtaaa aatcaatgaa ctacgtcaat ggatggcaca acaccaatta gaattggctt
     9601 atatcagcga ccctggccac atcgcttact tttccggata tgaaagtgat cctcacgagc
     9661 gtgtactagc tctttttatc cctctggaac aagatccttt ccttttcact cctgctctgg
     9721 aagttgaaga tgctcaaaac agcagctggg cgtacgatgt acgtggctac ttagacagcg
     9781 aaaatccttg ggagatcatc ggaaaagagc ttcgcgcacg ctatgggcaa cctggtaatg
     9841 taggtattga aaagaatgct ctgactgtcg atcgcttcga tgctatctct gatatcttga
     9901 caggtacaga ttcttacacc gatctaactt ctattatcca gcgtctgcaa ctgatcaaaa
     9961 cggtagaaga aaaagacaaa ctgatcgaag ctggtaaatg ggccgatgtt gcctttgaaa
    10021 ttggcttcaa aacagtaaaa gaaggcgtaa ctgaacaagc aattatcgca gaaatcgaat
    10081 accaattaaa aaaacaaggt gtttcacaaa tgtctttcga cacattagtc cttgcaggag
    10141 caaatgctgc aagccctcat ggtacaccag gaagcacgaa ggtttcacca aatgaactag
    10201 tgttattcga tcttggcgtt atttggaatg gctattgcag tgatgctact cgtacagttg
    10261 cttatcaaaa accaactgaa ttccaagaaa aaatctataa catcactctt gaagcacagc
    10321 ttgccgcaca agaggcagtt cgcccaggtg tgacagcagg tgaacttgat caaatcgcac
    10381 gaaacgtgat caacagctat ggctacggcg aatatttcaa tcaccgttta ggtcacggaa
    10441 tcggaacaac cgtccacgaa ttcccttctc ttgtagaagg aaacgactta gtgattgaag
    10501 aagggatgtg cttctctctt gaaccaggaa tctatattcc tgaaaaagta ggggtacgta
    10561 tcgaagattg tgtgtatgtg acaagtgatg gttgtgtgcc attcacgact acaccaaaag
    10621 aattacttgt tttagagtaa tgaaaataaa aaaggtgaga gctggtccgc tctcaccttt
    10681 tttattctag aaatgagtaa tcttagtgat tggtagtcga atctgtcatt gtatctttca
    10741 tttctttttt agcttctttt gcatcttctt ttgcatcttc ttttgccatt ttcaacattt
    10801 cttttgtgtc atctgcaact ggttttgctt ctgacattcc ttcattgacc gtctctcctg
    10861 ctttatctgc agcatctttt acatcgatca tgatatcttc tgaagtatct ttagcaatac
    10921 tacctaattc gtctgctggc tttttcagat cttctgctgt ttttttgaaa cgattagata
    10981 aatcagctga ttgttctttg aggtttaaca agatatcatc tgattcgctt cctgcttcct
    11041 ctgctgattc acttaccttc tgcttaacat tgcctgctag atcccctgct tgttgagaaa
    11101 gatcttgcgc tttttctttt gcaatacctg ttaactcaga tgatttttct tttgccatat
    11161 ctgtgtaatc tgaagccatg tccatcaaat catctgcttg agcagccaat tcatcccgaa
    11221 gttctttacc agatttgggt gctaacaata atgcggcgat tgctgcggct gtacctccaa
    11281 ctactgcacc taataaaaat ccaccttttt tagccattct tattttcctc ctgtcgtttc
    11341 agtatgtttt ttgtttttaa aaatttcatc gctgttccac cgacttttcc ggcaacagtc
    11401 gctttagctg tcgtttttcc gattccgccg actttcgtca cgatgttttt actagaatga
    11461 ttaagttccg atacactttc acttaaatca gcaactgctg tgaacaatgg atcgatcgtt
    11521 gcgacttttc cattgatatc caagagcagc tcattacttt tgactagcaa gccttctact
    11581 tgtctagata aaagatctac gtcttgtgtg acgatctcaa ttgtcgtatt tgcttctgcg
    11641 acagtctggt ctactttttc taaagttttg gaaacttgca acaatacgcg aacgatgaaa
    11701 acgacaagta tcgcaaaggc gattgctgcg atcaatcctg caatttctcc tcctgacatg
    11761 atcaaactcc cttcttgtct ctcttttcta taaggatagc attttcgtgt tttttaaaca
    11821 aataaaaacg agcttcaact actttttatt caattagcag ctgaagctcg ttagaaaaaa
    11881 agattatttt ttcttttctt ctttgatttc tgttattcct tttggattat gattgatctt
    11941 gcttccttct gcttctaatg ttttacctaa agacatgatg tattcacgca aaggtgcccc
    12001 tacttctgga tgtgtcagac catattcaat acttgttttc ataaaaccaa atttgtcacc
    12061 aacgtcataa cgtttacctg tgaatttacg ggcaaatacg cgttgtgttt tattcaacgt
    12121 atcgattgcg tcagttagtt ggatctcatt tccagcgcct ggttgttgtt ctgataatac
    12181 atcaaagatt tctggagtca ataagtaacg cccaataata gctaaatcac ttggtgcttt
    12241 gcttggatct ggtttttcaa caaagttttt gacattgtat aacccatctt ccagtacttc
    12301 ccctgggttg atgattccgt attttgaagt ttcatcgtga ggaacttgca taacggcaat
    12361 cgtagaagcg tgtgtacgct catagtcatc gatcaattgc tgtgtcaatg gtacttcgtc
    12421 ttccatgata tcatcgccta gcataacgac aaacggttca tttccaataa aggctcttgc
    12481 ttgaagaacg gcgtgtccta acccctttgg atgagattga cggatgaaat gcaaattgac
    12541 atctgtcgtt tcttctacta atttcaataa ttcggttttt cctttttcac ttaggtttgt
    12601 ttctaattcg atatttgaat caaagtgatc ttcgattggt cgtttagctt ttcctgtaac
    12661 gatcaagatg tcttcgattc ctgatttcaa cgcttcttca acgatgaatt ggatcgttgg
    12721 tttgtccacg attggcaaca tttcctttgc catcgccttt gttgctggta aaaatcttgt
    12781 tcctaatcct gctgctggaa taactgcttt ttttactttc atatacattc gctccttcaa
    12841 aaaattgtta atgagaattc attttctttt ttcccatcac gtaacatgat ttcttttgcg
    12901 gctgttttga tttctttttg ttcatacagc acttcataga ttgtttgagt gatgggcatt
    12961 tcgatttcca tcatttgggc tagttccatc gctgcttgag tagtattcac accttcaaca
    13021 accattccca tattggctag cacctcttct agaggctgac cttgtcctaa caagttgcct
    13081 gcgcgccagt ttctggaatg aacacttgtg caagtaacga ctagatcacc tactccgctt
    13141 aagccaataa aggtcagcgg gttggcaccc attgctacac ctaaacggct gatctcagct
    13201 aatccgcgcg tcatgattgc cgcttttgca ttatcgccaa accctaagcc gtgaatagca
    13261 ccagctccaa tggcaataat atttttcaaa gctgctccca tctctacacc aatcacatca
    13321 gggttggtat agattcgaaa ataatggttc atgaacagct cttgtacata ttttgctgct
    13381 tttacatttg tgcttgccgc tgtgatcgtc gtaatgtcat gctttgctac ttcttccgca
    13441 tggcttggcc cagaaagaac agcaacagct tttctttttt ctgtcgggat ttcctctaac
    13501 aagatttcag agattctttt atgcgttccc tgttctaatc ccttacttgc gtggatgatc
    13561 actggcttcg tattcaattt ggcaactagt tcttttgcaa cagaacgtat cgctttcgtc
    13621 gggataacga atagtaccgc atctgcatct tttactgcat cttccatatc ggtgaatgct
    13681 cggatggaat cgggtagatg atgatctggt aaataacgtt gattggtgtg ttgggtgttg
    13741 atttcttcta cttgtgaagc acgatgtccc caaatccgaa cgtcatgtcc gttttcagcg
    13801 aggacttgtg ctaaagcagt cccccaagag cctggtccta aaaccgctac tctataattc
    13861 attttaccac tcctgaaatt tttttcattt ttaacgctct gaaaatcaaa taatttgttg
    13921 attttttcct aatgagcggt cataatccgg taaacttcct ttttttctac gccaaataaa
    13981 cagaatcaat gtgccgacaa ataaaacagc agacaataat tgagaaatgc ggatcacacc
    14041 taaaaagtaa aggctgtctg tgcgtaaccc ttcgataaag aaacgtccga aactatacca
    14101 cattagataa cataaaagga cttctccttt tttcaaaaga tgtggtttct tccgcaaaac
    14161 caaaagcaga ataaatccaa tcaaactcca aagtgattca taaagaaagg ttggttgtcg
    14221 atagattcct tcgatataca tgttatcaat gatgaattgc ggtaaatgca gactttctaa
    14281 gaaacttcgt gtcgtgtctg gaccatacgc ttcgtggttc atgaaatttc cccatcgtcc
    14341 gattgcctgc gccaataata cgcttggtgc agcgatatct aaaaaggtcc acaaagaaat
    14401 aaaatgatat ttggtaaaaa agtacagtgt gattgcacct gcaatcaatc ccccgtaaat
    14461 cgctaatcct cctgaacgga tattgaatat ctggattgga tcagctatat attggggaag
    14521 atcgaataaa acataataca agcgggcacc aataatggaa atcggcaatc cccacaacat
    14581 aaaatctgtc acgtcatctg cttttaatcc tacccgaaca gcttctctgc tgctgagcca
    14641 catggcaata atgattcctg atacgataat caatgcatac cagtagatag gaattcctac
    14701 aagctcaaaa gcaactcgat tgatttgtgc taacatattg tcactccttg ctagtcattg
    14761 cctgagtttt cttcaatcaa gcgacttaat ctttcttcaa atgttttcgt tgcgtcaaag
    14821 cccattgtac gtgcgcggaa attcattgcg gctacttcaa tgatgattgc aacgtttcgt
    14881 cctgttttga cagggatttt tatttgtgga atgcccactt ccgcgatttc aacggtcgtg
    14941 tcttcagagc ccagacggtc gtatttctta tctttcgccc atgcttctag ataaacagct
    15001 aactggactt gcatagagcc tcgtaccgca ctcgcaccga aaagattcat tacatctatg
    15061 ataccgatcc ctcgaatctc aatcagatgt tccaggattt taggaggttc tccgattacc
    15121 gtcagttcat cttgctgata gacatctact cgatcgtctg cgatcaatcg atgtccgcgc
    15181 ttaatcagtt ccaacgccgt ttcacttttc cctatcccac tgtccccttg aattagtaca
    15241 cctaatccgt agacatcgac taacacaccg tggacacttg ttcgaggggc taagcgtcca
    15301 tctaaataac tagataattc acctagcagc cgggacgttg agataggcga acgcagcaca
    15361 gctatcccat tttcattggc tgcttggatc atttcttccg gagccggcaa tcctcttgaa
    15421 ataataaatg cgggcatatc ttcactacat aatcttctca taatgatcaa acgctcttct
    15481 gggatcatcc gttccgcaaa actgatttct ttactgccaa atagctgtaa acgattatgg
    15541 gaataataat taaaataacc ggtcaactct agtcctgggc gtgagatgtc cccagtcacg
    15601 atgacgcggt ctaaactttt ctcgtcgcct gtgacgattt ccaaagataa tttttttact
    15661 aattggctaa cttttacaac ttctgacatt catgctacct catttctatt agagtgttct
    15721 ctatcatttt atcattgtct agccaaaagt actaccaatt aaaaaaaacg tttttattta
    15781 attaccaatg taatagaaaa cgcattcttt attctaaaca aaaaa
//
LOCUS       DASOUD010000061        15334 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.061, whole genome shotgun sequence.
ACCESSION   DASOUD010000061 DASOUD010000000
VERSION     DASOUD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.061"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(22..252)
                     /locus_tag="U4A29_001774"
     CDS             complement(22..252)
                     /locus_tag="U4A29_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680898.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="U4A29_001775"
     CDS             complement(688..1254)
                     /locus_tag="U4A29_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO0680899.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="U4A29_001776"
     CDS             complement(1254..2210)
                     /locus_tag="U4A29_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO0680900.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="U4A29_001777"
     CDS             complement(2210..2866)
                     /locus_tag="U4A29_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO0680901.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="U4A29_001778"
     CDS             3236..5254
                     /locus_tag="U4A29_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO0680902.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5324..5530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5681..8095
                     /locus_tag="U4A29_001779"
     CDS             5681..8095
                     /locus_tag="U4A29_001779"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO0680903.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="U4A29_001780"
     CDS             8443..9996
                     /locus_tag="U4A29_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEO0680904.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="U4A29_001781"
     CDS             complement(10377..12161)
                     /locus_tag="U4A29_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680905.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="U4A29_001782"
     CDS             complement(12176..13897)
                     /locus_tag="U4A29_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680906.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="U4A29_001783"
     CDS             13997..14758
                     /locus_tag="U4A29_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO0680907.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4993 a         2801 c         3004 g         4536 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    15301 aaaaatcaca aaggttattg ttatttttat atat
//
LOCUS       DASOUD010000062        15139 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.062, whole genome shotgun sequence.
ACCESSION   DASOUD010000062 DASOUD010000000
VERSION     DASOUD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.062"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..2377
                     /locus_tag="U4A29_001784"
     CDS             5..2377
                     /locus_tag="U4A29_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680908.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="U4A29_001785"
     CDS             2438..3898
                     /locus_tag="U4A29_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEO0680909.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="U4A29_001786"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="U4A29_001786"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEO0680910.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="U4A29_001787"
     CDS             6129..6278
                     /locus_tag="U4A29_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680911.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="U4A29_001788"
     CDS             6353..6946
                     /locus_tag="U4A29_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEO0680912.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="U4A29_001789"
     CDS             6959..7858
                     /locus_tag="U4A29_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEO0680913.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="U4A29_001790"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="U4A29_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO0680914.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="U4A29_001791"
     CDS             8724..8984
                     /locus_tag="U4A29_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680915.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="U4A29_001792"
     CDS             9429..9635
                     /locus_tag="U4A29_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEO0680916.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="U4A29_001793"
     CDS             9683..9886
                     /locus_tag="U4A29_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680917.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="U4A29_001794"
     CDS             9902..10339
                     /locus_tag="U4A29_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680918.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="U4A29_001795"
     CDS             10332..11039
                     /locus_tag="U4A29_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680919.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="U4A29_001796"
     CDS             11419..11598
                     /locus_tag="U4A29_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680920.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="U4A29_001797"
     CDS             11923..12186
                     /locus_tag="U4A29_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEO0680921.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="U4A29_001798"
     CDS             12180..12530
                     /locus_tag="U4A29_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEO0680922.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="U4A29_001799"
     CDS             complement(12554..13168)
                     /locus_tag="U4A29_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0680923.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="U4A29_001800"
     CDS             13482..13904
                     /locus_tag="U4A29_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680924.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="U4A29_001801"
     CDS             13914..14117
                     /locus_tag="U4A29_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680925.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="U4A29_001802"
     CDS             14328..14912
                     /locus_tag="U4A29_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0680926.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5570 a         2451 c         3062 g         4056 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttagtt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 atagggacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctctagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc tactgataac
    15001 ttattctcaa atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa
    15061 aaaagtccgt actctaattt gcagtctctc actcttttag agtgagagtt tttattagat
    15121 tttcaaatta attagtaaa
//
LOCUS       DASOUD010000063        14993 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.063, whole genome shotgun sequence.
ACCESSION   DASOUD010000063 DASOUD010000000
VERSION     DASOUD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.063"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(82..234)
                     /locus_tag="U4A29_001803"
     CDS             complement(82..234)
                     /locus_tag="U4A29_001803"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEO0680927.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(425..1303)
                     /locus_tag="U4A29_001804"
     CDS             complement(425..1303)
                     /locus_tag="U4A29_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO0680928.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1342..2460)
                     /locus_tag="U4A29_001805"
     CDS             complement(1342..2460)
                     /locus_tag="U4A29_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEO0680929.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2457..4556)
                     /locus_tag="U4A29_001806"
     CDS             complement(2457..4556)
                     /locus_tag="U4A29_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEO0680930.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETMNYFAQLFGQLSMSDDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACSGLAEIFEALEQLSLSERYKDIACKLKESICKQMTIDGTF
                     GEQFLEGIGDEEKTTYSVKNYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDPLYQNTMTFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNH
                     QAFVTRLEELICLADLDGSWWWWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIYIQRRKR"
     gene            complement(4607..5428)
                     /locus_tag="U4A29_001807"
     CDS             complement(4607..5428)
                     /locus_tag="U4A29_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEO0680931.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5425..6234)
                     /locus_tag="U4A29_001808"
     CDS             complement(5425..6234)
                     /locus_tag="U4A29_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0680932.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6255..6749)
                     /locus_tag="U4A29_001809"
     CDS             complement(6255..6749)
                     /locus_tag="U4A29_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0680933.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6761..7249)
                     /locus_tag="U4A29_001810"
     CDS             complement(6761..7249)
                     /locus_tag="U4A29_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO0680934.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7326..10022)
                     /locus_tag="U4A29_001811"
     CDS             complement(7326..10022)
                     /locus_tag="U4A29_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEO0680935.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10450..11523)
                     /locus_tag="U4A29_001812"
     CDS             complement(10450..11523)
                     /locus_tag="U4A29_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO0680936.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11520..12347)
                     /locus_tag="U4A29_001813"
     CDS             complement(11520..12347)
                     /locus_tag="U4A29_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680937.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12348..13154)
                     /locus_tag="U4A29_001814"
     CDS             complement(12348..13154)
                     /locus_tag="U4A29_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680938.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13166..14251)
                     /locus_tag="U4A29_001815"
     CDS             complement(13166..14251)
                     /locus_tag="U4A29_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680939.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14268..14810)
                     /locus_tag="U4A29_001816"
     CDS             complement(14268..14810)
                     /locus_tag="U4A29_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0680940.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         4557 a         3319 c         2437 g         4680 t
ORIGIN      
        1 cttttttttc ttgctatctt tttataatca caactctcta taatagaatt acgatttatt
       61 ttatacggct gaaaaaatct atcaccaaaa aaattcaaac attaagccaa ggattatgaa
      121 gtcaacattt cgaaaaatga acatacattg gtaagcaact ataactatta ccacagacat
      181 aaagtaacac aatatattac tatttaagtg tacgccacca gtgaatatcc tcaaggtata
      241 cagcgcacaa caaaaagaaa aaaggaaacg cagaagcatt tcctttcagt gatttaatga
      301 aaacaacacg atttgactta gagccttttt atttgaattc attttcgctt tatccaactt
      361 catttgatca tttgattact cggttaaata cttttgacat gagtatcaat gaaacattca
      421 ttaattatgg ttgcttattt cttaccgctt ttctggccaa tgctaatccg ccgattattc
      481 cggcattatc acctaattcg ggcgtaataa tgtagctatc aattggtggt atttcaacat
      541 atccatttat aagctcaaca aatttatcct gaactttttt cttcatgtgt tcttgtttca
      601 ttacaccacc gccaaaaata attctatctg gcgaaagcat cagtgttgta ttgtatgcac
      661 attgggcaag gtaaaaggct tcaatctgcc aaaacgaatc gtcagccaat aaattctggc
      721 cttttacacc caatcgtttt tcaatcgctg gtcctgctgc catcccctct aaacaatctt
      781 ggtggaaggg acagtttcct gaaaaattgt cctttggatg tcgcttgatc aacatatgcc
      841 ccatttccgg atgactgaac ccttccacga aagtcccatt gaacaaggca ccacctccga
      901 tacctgttcc cacagtgaag tatgcaacac ctgctaaccc tttgccagaa ccagcaacat
      961 attcaccata gcaagccgca ttgacatctg ttgtccattc aatcggaata ttgaaatatt
     1021 gctttagcgt tcctacaaaa ttgaaatttt gccaagcaag ttttggtgtt gatgtgatat
     1081 gaccgaaact cgttgatttg cattgaatat caattggacc aaagcagccg accccgatgc
     1141 ctgccaattg ttccttaaaa ggtttaaaaa aatcaatgac ctgcttcatt gtttcaactg
     1201 gagttgttgt gggaaaactg actctttcca aaatcgttaa ttcctcagta ccgactgcac
     1261 agacaaattt agtccctcca gcttcaattg ccccatatat catcataggt tcctcccttc
     1321 gaatatgtga aaaacacttc attaacgcgg caagcgttct cgatcagtga aatgacgttt
     1381 agtatttcct gaagcttctg ttgaatacca ataggcgaca ctagcaacat cgtcttgccg
     1441 ctcaaataag cgaacatcat cgttgccgat ttgttgtaaa gtgaccgata aatcttcggt
     1501 aaaacagatg gggtcttgaa gatgccaacg ataaagacca tgtcgtggca ttgcgtcgtc
     1561 accaaaggca tgaacggcat tcaattttcc cgtttcaaaa cgatctcttg tggcatctct
     1621 atttgattgg taaggatacc ctaaaaaggc tgttgaatag tttttcgcat gtggacgtcc
     1681 gaactcatcg tactcatgaa aggcccacgc cccgccaaaa taatcttctg aacctgttga
     1741 ggttacggtc ggatactcac tatccccatc aatgtagaat ttaaactcgc cttcgcccca
     1801 ccaataacgt tctaggcagg taagcgcaag gtaagtaccc acgtaatagc cttgaccctt
     1861 taatcgatca attaacttat aatcctcctg taatttggta atgcgttccc gatgccaatt
     1921 tgcgtgaaaa tataggctat tttcttccaa ttcgtccacc aaagcgtaat ttatcgtata
     1981 aaaaaatgag gacatatcct tcggatgtaa attctcgatc gtaattttag cggatgtttg
     2041 gaaaggcatt tcgaaatagg aattaaagcc ccccgtagga ttaaccacta taggcaacga
     2101 gttcacctcg caacgctctc caaacccatt acaaaagaaa tcccctaaag gactttcaac
     2161 agccgggcta tccgacccat cccaataaat tcttaagatt aaatcacgta aaacaaaact
     2221 gccaaattct gttcgatcac gaatcgtcat ccacatatga cgaattattc ccgttccagt
     2281 aatatccgca atcacagttt cttcaccaga agtcagcgga attgcgccac gacctttgcg
     2341 gcttggtcct agctcgcttg gcaacatcgc agctttacct ttctcgcctg ttggattttc
     2401 gggagtgatt gctcgactct ggattttttt aaattgggta atatttttta gtatactcat
     2461 ctttttctcc tttgaatgta aatcgttttt cccttttcta cgtgaagaat ttttttcgta
     2521 tcacttattt tcaatggctt ttctgacaaa tttgaaatac tcaactcgga ttgcgtacat
     2581 tcaatcgaaa tgaaggcttg accgaacttc aaatttttcc aagaaaacgt taaattatca
     2641 acaggatcaa tttgcaatac tccttttttg aatttcaatc cgaaatattg actgatcatt
     2701 atactgacaa aaaggcccga tgcccagccg catttgccgc agccaaagtc tcgcacaact
     2761 tctccttgtt tagcctctaa acgatacggc caccaccacc atgaaccgtc taaatctgcc
     2821 aaacaaatca gctcctctaa tcgggttacg aaggcttgat ggttgtgcat gtcactcatt
     2881 aaaacagtga taaagccagg aaaagtggcg ccggattcta atccccaagt gatgcctttg
     2941 atttcctcgc tataagttgg attgaaagaa ctagcagaaa aggtcatggt gttctgatat
     3001 aaaggatctt tattatctaa aaattgataa aaaggcatca acgtagtgtc tgattcttct
     3061 ccatcatgca tcaataaatt gattttccca tccacaatca catcacttaa aaagattaac
     3121 ccttgctcaa gaatcggttt ttgatagttc ttcactgaat aggttgtttt ctcctcatca
     3181 ccgatcccct ccaaaaattg ctccccaaaa gtaccatcta tggtcatttg tttacaaatt
     3241 gattccttta gtttacaggc gatgtctttg tatcgctcgc taagactcag ttgttctagg
     3301 gcttcaaaaa tttctgcaag tccactacag gccttccaaa gacaaagatt gctgccggta
     3361 tgatactttc caagtgcaaa ggcgtctgat aaccaaacag atggaaaaag cattggttcc
     3421 cctgaccgac gttgagctaa tatgtcctca ataattcttg aagcattcag tagaacctgc
     3481 ggacaccgct caaagaattt tgtatcccca gtatgttcaa aataaagact agccagtaac
     3541 gcactggata acgaattact cagagaatga ttgatacctc ctgtaaattt tgaaccagga
     3601 aacttaacgc catattgatc aaaccataga accgtttttt gacataactc cggttcaaac
     3661 atggtgaatg gcaatgatgc gtaatacata tccttattcc aaattcgatt agtagcagga
     3721 tagctgcccc aattgctgcc gacgacctgt ccctgtcgat tcataccgaa actagaaaag
     3781 ctttgataaa gtgcacgacg atagagatgg acaactctat cgtctgacat tgacagctgc
     3841 ccaaatagct gagcaaaata gttcattgtt tccttcagcc atttttcgat atcagattcc
     3901 tgaaaagtca ttacttcttc aaaagcattt gggtccacaa aagcaatggc gaagcttgcc
     3961 acttcctgtt gtgcgatcgt gtccttattt cctttttgag aaatcaacac attttgctga
     4021 tccgaatact tctcttggta caaaggcaca tctttcactg aaacttgaag gctttcgtgt
     4081 gtttcattca caatgtaaac ctgttgaaca agcatactta aacgcttatt agccagtatt
     4141 ggagcaaagg atactacaat gaccttaaac gtcgaataat gatgaatata tctaggcaaa
     4201 cagtcttgaa tcacatcgac ttcaatacga tgatccgatt ctgacaaatg gtagacaatc
     4261 ccgtttactt caacctttaa ggataacgaa ttggtttgag aaagctgttt attgacccaa
     4321 acacctggtt tttcctcagt tagtaaattt tctcgataaa aggtcaacgt ttgaatcgtc
     4381 ccgcaaacat caaggtttcc taatgtcttt tgattactta catcaaaggt caatcccttt
     4441 acatcaggaa tattccgata gtagctgtat tcgtttgaag tagtcacatt tccgtcgttt
     4501 aactcagcat taaataatgg gtgtttgggt gaataaatta cttcattaat agtcatcttt
     4561 tccctccttg ctaaaaaaac tgcagcctag tagctgcagc ttcctactat gcgattccaa
     4621 gtaatgacaa taaaattccc aagacgacca ttccaaaaat aattttgatg acgccgactt
     4681 ttttatctag taatttgaaa cagcccaatg ttaccaataa cggtacaatt cctttaaaaa
     4741 tactatcgag gatttcttgg acattcatta gttcttcgcc cttcattgaa aagctcaaaa
     4801 cggtattgaa tttgaccata ctgatcgtca ttgcgcctac catgatcaga cccaagatac
     4861 tagcagcttt tgtcagaatt tcaattaacc cgttcgcata cagcttttca atatatttag
     4921 agcctaatga atagcctaaa acgccgccat agtatcgcat caaataatta ggtatattga
     4981 acataactaa aaagataatt ggtgccaaaa tatttccggc attggctaag ttgatcgcta
     5041 gtcccgcggt aacgacacgc caaactcccc agaacaatga atcgccaatt ccagccaatg
     5101 gtcccatcaa actcgttttg atcgcgttga tactttccac gtcaaaacca gctttttcag
     5161 aattttcctt ctccatcgat gccgcaatcc ccatgacaaa ggacgcgcaa ggaatggtcg
     5221 tcgaaaaata ctgtgtatga cgttccagcg ccgcagctcg atcttcttca cttttgtaga
     5281 aatgattaat gaagggcatc atagaatagg cgaaaccacc gccgcccatc gtcaccggat
     5341 tcaccgaagc atacatggtc atcgagcgcc aaaacatctt ccgaaccaat tttttctcat
     5401 ctttagaaat tccatcagct aatttcattg aaagaaatcc tcctcttctt ggtcaagcgt
     5461 tgcatttaca gcagcatcgc tttgaactgc atgaacagat atatttttta gttgtagatc
     5521 tctttgggct ataacaattg caataatcgt tccaactgcg gcaattgcaa tcaatggtaa
     5581 ctcgagatac gccataacaa cggccccaaa gaaaaaccac acagcaatat ttttactcca
     5641 aagcatccgt aacagcatcg ccatcccaac tgccggcagc agatttccag cgactgttaa
     5701 tccgttcatg atgacctctg gaatattatc caagacgctt ttgacaacat ctgctccaaa
     5761 taaaacggca aagaatggaa taagagcata aagcgcatag tggacaaacc acataacgaa
     5821 gtgcagattg atgattcctt tttgatcacc agattttgca aatttgaaaa atttagcagc
     5881 catcggtcca catacaaaag aaaaaatcat attcattaac tgtaatccta atactgctag
     5941 tggaaagaca atgggaataa tcgcatccac gtttcctccc attttgattg cgaacgctgt
     6001 agccaaccca gcagccaaag aaggctccgc cgtactagag attccgatat tgacgacacc
     6061 cataaaaatg gcttctagtg acgccccgac aatcaacccc gtctgcaaat cccccatcaa
     6121 cgctcctgct aacggagcaa caacaatcgg acgactcagc atggacatcc cgaacatttc
     6181 atgtccagca actccaataa aaacggccaa agcgactaca aatgcatcat acataatttt
     6241 tctccttcct ttttctaaaa atcactcaaa atagagatag ctgaacgttt ttccatcgcc
     6301 ggtgtaggct gaaaaacggt atctggataa agttcaacta attcttttaa gtcttgaatt
     6361 tctgacgtcg ttaacatcac ttcctttgat aaggttttct tatcttccga tggcggtgtc
     6421 attttcccag cattccccac gttcattaat tcgatttccg ttgggaaagc tttcgcaact
     6481 tttaccgcat ctttgacatt agggactaaa accatcaagc gcatgtcctt cgcccgcgga
     6541 tcttttaaca aatgaatcgc gtcatcaact gttttaataa tgactttgac attcgatggt
     6601 gcagccatct tcaatgtcat cttttgaata tcgttggtag ccgcctcatc gcttgctact
     6661 actaacccgg ttaaatttag actctttgtc caagtcatcg ctacttgtcc atgaattaat
     6721 cgttcatcaa ttcgcactgc tttaatcatt tttcttcctc ctaaaaaaaa tcttcctctt
     6781 catttactac atgtacctca tcaagaatgg tcatctttaa ttcctgttgg catttattta
     6841 ccagttcctg aatatcctcc tttgttaatt ccttggttgc caatactatt tcgagcagaa
     6901 ttggcagatt aaagccagac aaaatagtta cagcaatctc tttctccaaa aaggttttgg
     6961 taatcttctg attgacactt ccgccaaata aatcggtaaa aacaaaaatc tgatcctgct
     7021 cgttcacctg gtttacaaat acattcaact cctgttcaaa atcacactca tccacataag
     7081 cattaatcac cgtaatctct tgaaccttcc ccgtcagtaa ttgaatactg ctaagaaagc
     7141 ctgaagctaa gtgaccgtga gtcgctatta gaattttctt catatggtta attcctttct
     7201 ttctgttcac ttttatataa gcaattcctg tgccaacttt taaaaacatt ggtttaatca
     7261 cgttaattca cattaaaaaa agtgtctaac ctaaaaaaaa cagattagac acttttttag
     7321 acactttatt tttgaatata cggtcctaat aattcataga gcagcgaaag ctcataagca
     7381 ttgactttaa aattatattt catctccatt gattgaaaaa tacttctaga aacataataa
     7441 aattcatctt catctggttt caaatcataa ttcggttctt cagatttttc tgtccgagcc
     7501 aagaataaac gttccatcat caatgccgta tgcatataga gatttagttt tacacggcca
     7561 tttaaagtga aatgatagta attttcatat ttagaaatga ctgtttccac ttcactcatc
     7621 acgatcgttg ggttcagaaa gttcaaacgg tccgcaattc cttccaacga aaagaatttt
     7681 actagctcct gaatcaagcg gttaaattct gccggcttca tttcaggact caaaatatgc
     7741 tccaaattgg ctgctccatc accgtctaag atgtcataaa cattaatatt aggaattgaa
     7801 aaggaacgtg gaagatccga agtcgtgatg accaattttg tcttgtcaaa ataagtttca
     7861 tcattttggt cgattaattc acgtagttct ctgtagtcct tcgtgaaaac ttcgatggat
     7921 tcatccgata aatgatgacg aaaaacatct ttcaatttgt ccgaaatccc caaacccgac
     7981 atacaggaaa tgataatgtt gctactttga gaaaagcctt caaagtattg aacattgatt
     8041 gaatacttgt ctgctgcatc ctccgcaatc tgtttaaatg gcgcattcat cgtcatcttc
     8101 aagccaatat caagtgcaat ggaagtcgtc aaattattca gaagaagtaa ctcccctttt
     8161 aaatcgttct tgatctgtgt gtaaatttga tttaaagagc ccatatctac gatcagcact
     8221 agactatctg tattcacttg ccgtttcacg aaattccttg tcgattcgac aatctctgac
     8281 acattactct ctactggcat atccaacgct tcaaaaacat aattgccgca taattgattc
     8341 actactgctt gaatgctgct agccgtacta tctccgtgag caagcaacag gcccttcagc
     8401 tcgatggatt catctacata atcagataaa gtcacgacaa taagcagtct tagaaagttc
     8461 tcgtcctttt ctgagagcgg tggtaaatgc tccaagaact gattacataa gtaagtcgtc
     8521 cgaggtagat ttgcagctaa tgtacgattt atcccggtta agtctgtctt atagcttggg
     8581 gaaatgacat aggatctgct aataatttct gcaacaggtc cactcgtcgt caatccgtac
     8641 gtttttccta aaacggatac ccagaccttt ctaaacatct gaatcatcaa ttttctactc
     8701 attgataaaa aagaatcctt atcaatgtga tgcaccaaat gctgtacttc attctttatc
     8761 gaagaaaaat tttcattgaa attaccttca tttatcagct gagtaatttt ttccaaaatc
     8821 gaatcataat caaacggttt gattcgatcc gtttttatct tttgattttt ctttatcaat
     8881 aaactagcaa tcggcaagct ttctacttcg gattccagca tatttaactg aatcaagatc
     8941 tcatttttcc caatcgttaa tggttcttct tgtgttcgac tatacacatc cgcacaactg
     9001 attttaatga tattcttcag cttaccaata ttcccactaa agggatgaga taacattaaa
     9061 gacaaggctt ctcttgaaac aataatatct ttttgtaaaa catccgcttc attttgaaaa
     9121 aataattgaa tcagctctaa tcgttctcga atggggcgat cttcaaaagt tggcaacata
     9181 acactaattt gaatacgccg atcaaaggtg tctaataaat aattttcacc tttttccgtc
     9241 gtggcgaaaa taaaacgaac cttagcagaa tgccaattta cattttcacc gactggacga
     9301 tagtttccag tatcaataaa tagaaagagc ttttcttgat tttctttcga taggcgatgg
     9361 acctcatcga gaaaaagata gccgttgttg gccgttgcca ataaaccatc ggtatccttt
     9421 tcagcccccg taaaggcccc cttcttgtat ccaaacagtg ttgccgaaag cagttcggga
     9481 ttgtccgcat aatcggcaca gtttaagata gcaaatggtg catctttttc aataatttgc
     9541 tcatggatag cgtattcaaa aatttttgtg gctaaaaaac tttttccaac acccgtggct
     9601 ccatttatta atacactgag tccgttaggc ggatatttga cagcggctat acatttttga
     9661 atgacctctc tcagacttcc gtcatgacca actacagaat gaaaggcttc gtttatttgc
     9721 tccgtattat ctacaaccgc atattcatct aaaggcttcc acaaaacagg ctttcccggt
     9781 aatttctcga cctttttatc ttccactaat ctcgtcaaat aatggctggc attctgacga
     9841 gagataccta gattcttcga aattttctcg gcagtgacag aagacatatt ccctgttagt
     9901 cgtatcagct catcaaaaac tcgatcttta gcgctctttt tcattttttc ctccagttta
     9961 atgtgtctaa aagcataata aactatcggc tgcaatatgt ctattctata tttgtattcc
    10021 aatttttact ttgaaacaca aatagtcgaa tatttgcctc agatatctga caaaataatt
    10081 tatctaccaa tttagttttt atctaatatg atttttatgt tttttcatag ctacaaatat
    10141 tgaactggat tcatatatta gttttattaa tagattacag aagggttaag tatatttgca
    10201 tagtttccaa ttactttatc cttctgtaac tttttcaatc ttagatttac tagcccgtct
    10261 cgggccctat tttacaatgg ttttgtccac ttttctctgg tagaaaaacg tggtagaaaa
    10321 aataaaataa cacaaaaaaa gcggcgtttc atccgctttt ggtagaagat tggaaaactc
    10381 agatagaaaa aggacgattt ttagctcttt agaacgttga taagtcccca ttcttactga
    10441 gttttgccat tatctcctat acatcttgaa ttctaagaat aggtcattat agatgcctag
    10501 gtatttcgat cctagatctt tgtagacttc aagatgggag atcgtgtcat cagatggata
    10561 gaattgttcg tccgacgaaa tgtcttttgg caataacgct tttgcttctt catttggtgt
    10621 agaataacca atatactctg cattttgtgc cgcattttcc ggttctagca taaagttgat
    10681 gaaatcatag gcaccttctt tatttttcac ggtttttggc ataacgatat tatcaaacca
    10741 aagattggat ccttctggtg ggatcacata atgtaaatgt tcattttccc acatcatatc
    10801 ggcagcttcg ccagaaaatg tcactgccac ggaggcttct tcattgatca tatacatttt
    10861 gatctcatct gccacgattg ctttgacatt tggtgttaaa tgattcaatt tatttgctgc
    10921 ttctgtcagc tgtgccatat tcttactatt caaagaatag cctaaactat tcaaagaaag
    10981 ccccataact tctcgtgcac cgtcaatcag catcaaactg tcttttaatt ccggcttcca
    11041 caaatcatcc caatgcttaa tttgatttgc atcaaagaat ttatcgttat atacgatccc
    11101 taaagttccc caaaaatatg gaatcgaata ctgattatct ggatcaaaat ccaagtctaa
    11161 aaatcgtgga tcaatattat ttaatccttt gatcttcgaa tgatctaatg gaagtactag
    11221 tttttctttg atcatttttt ggatcatata ttcacttggt atagcgatgt cgtagtttgt
    11281 cccaccttgc tggatttttg tgaacatcgc ttcattagaa tcaaatgttt cataattcac
    11341 tttgtatcca tattcttttt caaatttcgt gatcaaacta ggatcgatat aatctcccca
    11401 gttatagatg gtaagtacct ttgctccact catgccactt gttttttcaa gttgacgaac
    11461 accaagcagc agcaccgcaa taatgatgag aatacctaaa aacagagatt gtaatttttt
    11521 catcgcagcc gtgccacccc ctcgcgtact cttctgtttt tcttcagccg ttttggacgg
    11581 ttttctttac taataaagta gtatccaatg accaatatca tggagaacaa gaaaactaac
    11641 gcacttagcg cattgatttc taaactgatt ccttgtcttg cacgagaata gatctctact
    11701 gagagcgtcg taaagccatt tcctgtgacg aaaaatgtaa ccgcaaaatc atcaagagaa
    11761 taggtaaatg ccataaaata tccagctatg attcctggag aaagataagg caaaatgata
    11821 tttttgacta cttggaaatt attagcacca aggtctctag ccgcatcgac cattgaatcg
    11881 ttcatttcct gcaattttgg caagaccatc aagacgacga ttggaatgct gaaagcaata
    11941 tgggaaagca atacactctg aaaacctaat ccgaatccta gtagtgtaaa gaaaatcaag
    12001 aaactagcac cgataataac atcaggagag actaataaga tgttattcat gctcaacaac
    12061 gctgtccgtg cacgtcgttt tttcgtgtaa tagattccca tagccccaaa tgtcccgatg
    12121 atcgtggcga tcaatgcaga taagaaagcc agcatgaacg tattcaatac gataatgatc
    12181 aaacgtgtat cttcaaaaac agcttcataa ttttcccaag taaatcctgt aaagctgttc
    12241 atcgttcctc cttcattgaa ggaataaaaa atcagataga agatcggtgc atataagaat
    12301 aaaaagacga tccctaaata aagatacgac catttaaatt ttttcattta tgagcgacct
    12361 ccttttttct tctcgcctgt caggagcatc acgatgaaca tagcaatgat taaaatcaca
    12421 ccgattgtag aacccatccc ccagttttgt gtaaccaaga aatgctgttc gatagccgtt
    12481 cctaaagtga tcacccgatt tccgccaatc agacgagtca gcatgaataa agaaagagaa
    12541 ggaataaata ctgcttggac accacttttc acaccattta atgataacgg aaaaatcact
    12601 cgtcggaagg tctcaaagct attcgcgcct aaatctctac tagcactgat caaagaagga
    12661 ttcatctctt ccaatgcatt gaaaattggc ataatcataa atggaatctc gatatacgtt
    12721 gccacgaaca gaaaactgaa gtctgtgaat aatatctgtt tcggcccgat cccgataaaa
    12781 tcaaagaaat gattcacgct gccgtgcaca ctgaaaatcc caataaatgc gtaagcttta
    12841 agcagcagat tcacccatgt aggcaaaata atcagcatca gccataattg cttgtgcttc
    12901 agttttgtta aaaaataagc agtcgggtaa ctgatgatca gcgtgaaaaa cgtgatcaaa
    12961 aatgcatacc atacagaatt gatcgtcata gaaagcgatg tccccgacgt aaaataagat
    13021 agataattat tcagcgtgaa ttgcccattc atatcaaaaa aagattgata aatgatcatt
    13081 aatacaggcg cgatcacgaa taaaccgagc cacaaaatat aagggacgga atagatcctg
    13141 cgcattgttt tcattcttgt cccccctatt cttcgtagct gtcaagacgt gcatcgaatt
    13201 cttcttcaga ctcgttgaaa cgcatgacat ggatatcttc tggctcaaag gccaatccta
    13261 ctttgcttcc ttctttagct tttttcgttg aatggaccat ccattcattt tgctgttcat
    13321 cataacagat gatttcataa tggacaccac gaaacagctg agtatcgacc gttacgacta
    13381 gttttccttt atctggtgtc gtgatcgtca aatcctctgg gcgtaaaaca acttcaactg
    13441 gttcatttag acgcatcccg ccatcgacac attcaaattg cttaccaaca aactcaacta
    13501 gattatcttc gatcattaca ccgtccacga tattgctctc cccaacaaaa tcagcaacga
    13561 aatgattgat tggctcatca taaatatcta cgggtgtacc actttggacg attttccctt
    13621 tattcatgac aaaaatctca tcactcattg ccaacgcttc ttcttggtca tgagtaacaa
    13681 aaataaacgt gattcctaat cgttgttgca gatcccgcag ttcatactgc atatctgtac
    13741 gtagtttcaa atcaagcgcc gataacggtt catctaatag caatactttc ggttcattta
    13801 caattgctcg tgcaatcgct acacgctgtc tttgaccacc agacatttca ctaatctccc
    13861 gtttttcgta tccaggaagc tggaccatac gcaaagcatc ttttaccttc ttttcgatat
    13921 ctgatttgga cattttcttg atcttcaatc caaaagcaac gttatcaaaa acattcatat
    13981 gaggaaaaag tgcataatct tggaagactg tatttacttg tcgcttatta gctggcaaat
    14041 cattcaaccg tttcccttca aaatagatgt ccccttctgt agcttcggtg aatccagcta
    14101 taatacgtag gatcgttgtt ttaccacagc cggaaggacc aagcagtgta taaaattttc
    14161 cttgttcgat ctcaaaactt acttttttta atactgtttc atcatcatag cgtttcacta
    14221 cttgatcaaa tgaaatgatt ggtttcccca cgtttttacc ccctatatta caaatatgat
    14281 tgcgttgcaa caatcaatac ttttgttcgt tttttgagat gattccttaa ttgatgttgt
    14341 tttgtcgctt gataatagat cgactgtcct gcttttgcca catagaccgt ctctcctaag
    14401 cttaactcta attccccttc taatacaaaa ataaacgttt cagataatga aggttcaaac
    14461 gtcttatatt ctcctgcacc ttcaaaagtc agcaagacag gctccatttc tttttcattc
    14521 gaatcagcca cgagccattc caattcatac ccgttttctt catcgaaata aaccgtatga
    14581 tcttcttttg aataaaccac ttgcagattc gctgcttctt gatgaaaaaa ttcttctgga
    14641 gtcacaccta agacttcaag aatagaaaag aatgtttcca ttgatggtga acttaaatct
    14701 cgttccaact gggaaatata cccttttgac aaatctgtgc gttcgcctag ttcttcttgt
    14761 gtcaaattct tttggatgcg caaatttctt aacttctcac caatttccat aatttcctcc
    14821 gaatcgaagt tttctaatag taaacttcct gtttactacc actcgtatta gtatacgaaa
    14881 tttattgaaa aaggcaaccc tttgtaaaat tttttttgct gaatatttct ttatcttttg
    14941 gaataaattc tagttgtttg attctttatt tttcacctaa tttttgtcta aat
//
LOCUS       DASOUD010000064        14105 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.064, whole genome shotgun sequence.
ACCESSION   DASOUD010000064 DASOUD010000000
VERSION     DASOUD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.064"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..241)
                     /gene="rpmF"
                     /locus_tag="U4A29_001817"
     CDS             complement(62..241)
                     /gene="rpmF"
                     /locus_tag="U4A29_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO0680941.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(299..868)
                     /locus_tag="U4A29_001818"
     CDS             complement(299..868)
                     /locus_tag="U4A29_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO0680942.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1054..1956
                     /locus_tag="U4A29_001819"
     CDS             1054..1956
                     /locus_tag="U4A29_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO0680943.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(1991..3775)
                     /gene="pyk"
                     /locus_tag="U4A29_001820"
     CDS             complement(1991..3775)
                     /gene="pyk"
                     /locus_tag="U4A29_001820"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO0680944.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3849..4811)
                     /gene="pfkA"
                     /locus_tag="U4A29_001821"
     CDS             complement(3849..4811)
                     /gene="pfkA"
                     /locus_tag="U4A29_001821"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO0680945.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(4979..8293)
                     /gene="dnaE"
                     /locus_tag="U4A29_001822"
     CDS             complement(4979..8293)
                     /gene="dnaE"
                     /locus_tag="U4A29_001822"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO0680946.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            8524..8709
                     /locus_tag="U4A29_001823"
     CDS             8524..8709
                     /locus_tag="U4A29_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEO0680947.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8771..9967)
                     /locus_tag="U4A29_001824"
     CDS             complement(8771..9967)
                     /locus_tag="U4A29_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO0680948.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10137..11573)
                     /locus_tag="U4A29_001825"
     CDS             complement(10137..11573)
                     /locus_tag="U4A29_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO0680949.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11866..12675
                     /locus_tag="U4A29_001826"
     CDS             11866..12675
                     /locus_tag="U4A29_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO0680950.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            12855..>14105
                     /locus_tag="U4A29_001827"
     CDS             12855..>14105
                     /locus_tag="U4A29_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEO0680951.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         4224 a         2943 c         2515 g         4423 t
ORIGIN      
        1 aaaataaacc tcaaggctcc atggttagcc ttgaggtttt acattttcta attaaaatgt
       61 attaagcttc ttttgacatt acgtccttac catcatagta accacaattt gcacatacgt
      121 gatggctttt tttcatttca ccacagtttg gacattcgtt taaccctttg attgtcaatt
      181 tgtagtgtgt acgacgtttt gcttttttag cttttgatgt tcttctagct ggtactgcca
      241 ttttgctaca cctccttgta aagtcttgta tctaaattag acacaatata ttccaatctt
      301 acttgttatc gtcgtctttt gatttagaat caaaaagttc agatagtttt gcaagacgag
      361 gatcaatttt attctcggct tcttccattt ttctgcgctg gtattcttct tcggaaacaa
      421 cttcccaatc attgcctttt ggaagttctg ttgctgcttc ctcttctttt gtcaataccc
      481 gcattggaat cgccaataag atgttgtctt caatcgactc tgctaaattc aataatggac
      541 cttcaattag caaaatctca tctgcagaca tttgttcatc tctactttgg aattgttctt
      601 ctgtcatgaa tacttcgtct acagaaaaat ccatgggcag ttcaacaggt tctagtgaac
      661 gtgttgaagg aactgtcaaa gtcgtctgca cagtgtaatg aacgatatag cctttcttat
      721 caactgagac caaaccttcg acttccactg gcgcaacatc aagtattaaa ttgtctcgct
      781 tcatgagagc ggctttcaag tcgagcgtct catgaaaagt taacggtgtc tcctggtatt
      841 ttctcaattc caataaagac catttcattt ttcatcactc ctaagcaaca aaagcaatta
      901 tacagggcaa aaagcatgtt gtcaatgaaa ttttcttgac accttgcagt tcataaaaaa
      961 acagaaaaat ctttattaga aagcctctgt ttgctatcat cctcagaatc atttacaata
     1021 atcattattc cattaaagga gcggatgatc ttcatgaaca cgaaagatat cgaatactat
     1081 gtccaaatcg tcaaacaaaa aaattttacg aaagtggcta attatttccg tgtgagccaa
     1141 cctactatta catatgcatt acaacgtctt gaaaaagagt ttgacaccgt acttattaca
     1201 agagatcgat cgcatcacga attgatcgtc acgccttctg gaaagcagct gtttctccat
     1261 gcacaaacca ttctccaaga gatttctact gcaagagctg aaatcactca gttgcaaaag
     1321 aaaaagcttc gttttggcat gcctcctatt atcggcaatg aatactttcc taaactgtca
     1381 agttacttta tcaagcatga attgatgaat caaattgaga ttaccgatgg aggctctcgg
     1441 gacttatacg ggatgatccg acaagggagg atcgatttag ctgtattagg ctcaacacag
     1501 ccaatcgtgg atcaatattt agaaacagag ttacttttgg acaaacgttt catgatcgtc
     1561 gtgccaccta cacatcctct ttccaagcag gatactgtct ctttcaaaga tatagcagat
     1621 gaacagtttg tactattgaa cgaacattat gtccaccccg cagcattcac taagctttct
     1681 aaacaaacac atactcatcc aaaagttgtt tatcaaagca atgacttgac gattttaaag
     1741 agtatggtcc gagaaaatgt gggtatcggt tttttggcag agattgctgt ccatcctgaa
     1801 gatcatttag tttgcctccc tctttcagat gaagttcagc ctcgcttctt gatttcattg
     1861 gcaaaacgaa cacagcagat cgtcacgcct cttcaagaac aagtagtcca agttatccat
     1921 gaattcgtta cagcacaaaa aaacagctct tcgtaaaaga agagctgttt ttatttttta
     1981 ttaagctaaa ttagatagct gttgtttcgc cacggtaaac gatgccgcga cgtgaatcaa
     2041 cggtaatcaa ttcaccttct gtaattgttg ttgttgcgcc tttagcacca acgattacag
     2101 gaatatcttg tgcaattcct acaactgcag cgtgagaagt caatccgcct tcttcaacga
     2161 taacagcagc tgctttttcg attgcaggca tatattcttt gtcagttgat ggtacaacta
     2221 gaaccattcc gtcttttgct ttttttacag cttcttcagc agatgaagca ataactgcat
     2281 ttgcaacgac agatttttgt ccaacgcctt gagcttctaa taatttagaa ccaatcattt
     2341 gaattttcat cacgttagtt gttccgcgtt caccaactgg tacaccagca gtgatcaaga
     2401 ttaaatcgcc ttctgatgcg aaacctaatt caacagcttt tttagctgct agttcaaaca
     2461 tttcatcagt tgttgatggt ttttcagcaa ctgttgggta aacgccccag tttaacatca
     2521 aaccacgttt tgtgcgttcg tcaaaagtaa cagccaagat atctgcatct ggacgatatt
     2581 ttgaaatcat ttttgcagtg tatcctgatt cagttgcagc aacgattgtt ttcacgccta
     2641 gattttttgc agcacgagct acagataaac cgattgtttc agttacatct gttttatcga
     2701 attcgttcaa ttggaatgta cctaattctg ataatgcatt ttcagcttct tggtcgatac
     2761 gtgccattgt tgaaacagct tcaactgggt agtccccgtt tgcagattca ccagatagca
     2821 ttgttgcatc tgtgccatca aatacagcgt tagcaacgtc agaagcttcc gcacgtgttg
     2881 gacgtgggtt ttcttgcatt gattctaaca tttgtgtagc agtgataact gggataccag
     2941 cagcattaca ttttttgata atgcgttttt gaaccattgg tactagttca gctgggattt
     3001 caacacccat atctccgcgg gcaatcataa caccgtcaga aactttgatg atttcgtcaa
     3061 tgttgtcgat accttcttga gattcgattt tagggaagat ttgaacatga gtcatgtctt
     3121 tttcttctaa gatttcgcgg atatctagaa cgtcttgtgc tttacgtaca aaacttgcag
     3181 cgataaagtc gatatcgttg tctaaaccaa aacgaatatc gtcagcatct tttggagtaa
     3241 tacctggcaa actaatagaa acgcctggtg catttacccc tttacgtgat cctagcatac
     3301 ctgcattttt aacttctacg actaattcac ggtttgcttc gtctttttca atgatttgca
     3361 tatcgatcaa accatcatca aataaaatgt gtccgccaac gtgaacatcg tcaaataatc
     3421 ctgggtaagt aaccgcgatt ttttcttttg aacctacatg ttcaggatcc atagagatgc
     3481 gtgtttgatc tcctacttca aatttgatgt atcctgcacg accaaaatca gcttctgttg
     3541 tgccttgaac agttgtacgg atttcagctc cttttgtatc taaaagaata ccgacatctt
     3601 ttcctgtctt tttaactgct tcacggacca tagtcatacg tgataattgt tcttcatggt
     3661 caccatgaga aaaattaaag cggaagacat ttgccccaga ttcaatcaat tgagaaataa
     3721 tttctactga gttacttgct ggtcctaatg tactaacgat tttggttttt ttcatttaaa
     3781 aaactctccc attcaatttt gtttatttaa agctgtacac agcttaaata gcttggtttc
     3841 tttcactctt aaaatgaaag tgaattattt agatcatata gtgataagtc tggtttatgt
     3901 ttgttgtttt caagtgtatc aatgatgtca gacgcaacga tttcgttgtc atggataccg
     3961 acacataatc caccttttcc ttcttgaagc aattcgacag cgtaagcacc gaatttactt
     4021 gccaacacgc ggtcgcgtgc gcttggtgct ccaccacgga caacgtgtcc caagatagat
     4081 acacgtgtat gatagtcacc aaattctgaa agtttctcag cgaattcatt tccgcccatt
     4141 acaccttcag ccaagatgat caagcaatgt ttcttcccgc gatcgcggcc ttcttggatt
     4201 tttttagcaa ctgaagccat atcaaaatca tgttctggga tgatgatttc atccgctccg
     4261 ccagccacac ctgaccataa agcgatgtct cctgcattgc gtcccattac ttcgataacg
     4321 aatgtacgaa catgagaagt cgctgtgtca cgaatacggt caattgattc tagtactgta
     4381 ttgattgctg tatcaaaacc gattgtgaaa tcagtcccag gaatatcatt atcgattgtt
     4441 ccaggaatac ctactgctgg gaaaccgcgt tttgtcaaag ccattgcgcc atgataagag
     4501 ccgtctcctc cgataaccac tagaccttcg attccgaatt ttttcaattg ttcgattcct
     4561 tttaattgac cttcttcagt tgcaaattct ggataacgtg cagaatacaa gaatgtacca
     4621 ccacgttgga tcttgtcacc tacatctgcc acatcaagac gacgaatatc tccagcaact
     4681 aaaccggcaa aaccataatt gatcccgtat acttccagtc cttcgtaaat acctttacga
     4741 acaacggcac gaacagctgc attcattcca ggtgcgtctc cgccactggt caaaattccg
     4801 atgcgtttca taataaacat tcacctcatt aaaatacttt atcctaagta tgacttaggt
     4861 ttacaaccat ttttgcgtta accacgacta acattctaac attatttttc tcacttgtct
     4921 tgatttacgg ataaaaaaat gaaaaaaaat gaaagcaagt agtatttttt accgtaagtt
     4981 atttgaaaac cacattatct tcgccaagca gataaataag ttgccgcttt atactcttgc
     5041 tgtcatccac ccaataagca tctgacagaa ctacttttcg ttgcgttttt tcgtgataca
     5101 taactactgg tatatatcct gggtgtttct gaaaaatcac ttgtaattgt ttcattatct
     5161 cgggatcttc ccgttcttct gtaatacgca aatagcaagt ttgtgaagga tactgggctt
     5221 ctaactggtt aggatcagca aagctttctc caattagttg cagttcctga ttatatttgc
     5281 tgatttcgct ccgcccttga acaaagatca ctcgatcaac ggcaaacaaa ttacgatatt
     5341 tacgataaag agttggaaaa actgtgacag aaatttctcc actactgtcg gttccttcaa
     5401 caaaggccat cagttctcct ttttttgtcc gaatttctct aacttccttg atcatcacta
     5461 ataaattgac tggttgatta gggacgatat ctgtaatcag ctgaacttgt ttagctaaac
     5521 gaagacgatc aaacccttct gcaggatgac ctgacaaata aatacctaag tactcctctt
     5581 ccagcttcaa gcgttcttct agtgtatagt cagcgacttc ttcttctttt aatgccatga
     5641 ttcccagcaa gtcaagactt ccaccactgt aaacgatatt ttggattttt ccttcgagat
     5701 caagcatcaa ttgtttcctg ttaggagata attcatcaaa aactcctact gctgtcagtg
     5761 attgcagtaa ctctaatttc agccaacgct tatcgatccg tatgaggaat tgatccactg
     5821 actggaacgg gccattttgt tttctttcgt ttaatatatc ttctacaaaa tcacgccgaa
     5881 taccttttat cgctgtcagt ccgaatctga tttcttcctt attgaccaac tcaaaactgg
     5941 aattactttg attaatggac ggcgaggaaa gtttgagttt atttttacgt gcttctgcaa
     6001 tatattcttt caattttgat gtgttatgcc gaacagaatt catcaggctg gtaaagaaag
     6061 cacctggatg atgggctttt agatacgcca tttggaatcc cacgaatgaa taagcaaatg
     6121 catgtgaccg attgaatcca tagtttgcaa atttttcgat atattcatat acggtcgctg
     6181 ctgttttttc cgaatatccc tgttgtaatg ctcctgatac aaaatgcgac ttttcttcat
     6241 ctaatacgtc ttttttcttt ttactcactg cccgtcgcag aatatctgac tgccctaatg
     6301 taaatccagc catagtcgag gcgatctgca tgatttgttc ctgatagacg atcactccat
     6361 atgtgttttt caaaatcggg atcagtgaat catctgggta gcgaatggct tcttttcctt
     6421 tcttcctggc aatgaaggta tctatatttt gcatagggcc tggtcgatac aatgcattga
     6481 ctgcggcaat atcttcgatg ttttctggat ctaatcttcg taaaacgttc cggataccag
     6541 cagattcaaa ttgaaatact cctgaggttt cgcctcgttg aaacaagagc agcgtttttg
     6601 catcatttag cgggatctct tttaatcgga tctcataccc ttctactcgc ttaacagctt
     6661 tcaaagtaaa atcaataatc gataagttgc gtagtcctaa aaagtccatt ttcaaaagac
     6721 cgattgcttc tacctcattc atcgtatact gggtcagaaa agcttcatca gaaccgcgtt
     6781 ggagcggaac tagttcaagt agattcttat cgctgataac tacacctgct gcgtgagtcg
     6841 aaacatgtct tggcaatcct tctaaaatca ctgcagtttt gaataaaagt tggttttttt
     6901 cgcttgcctg aactaattct actaattgat gcgaagtttt ataagcttgg gtcaatgtga
     6961 ttttcaattg attgggtatt gcttttgacc agcggtttgc ttcactttgc gataaaccaa
     7021 acactctagc tgcatctcta agaaccattt ttgctgccat tgtgccaaat gttgcgattt
     7081 gtgccatatg atattgtcca tacttttctc gaacataatt caatacttct tcacgtcgat
     7141 tgtcaggaat atccaaatcg atatctggca tcgtaaagcg ttcggggttt aaaaagcgtt
     7201 cgaacagcaa atcatattct aatggatcca catctgtgat ttctaacaca taggctacta
     7261 atgaacccgc agcagaacca cgacctgcac cggtaacgat tttgtttcta tgagcaaaag
     7321 ccattacatc ccatacgatc aagaaataat catcgaatcc catttgatgg atgacagata
     7381 actcgtagtc caaacgttct tggtattcct ttgtatagtc agtgattcgg cgttccactt
     7441 gcgaatagca taactctctt aaatattctc cagcttgttt tccattagga actggataat
     7501 gcggaagtaa tttctgatgg aactggaatt caaatgaaat cgattcagct aaacgctggg
     7561 cgcctattac agcttgggaa agacccgctt tttcgaatcg tttgatcatt tcttgttctg
     7621 aaagaagata gacatttgct gttagtggta aagatgcaga taaattttct aaaggtattt
     7681 ggcttccctc tttgatatgt ttcatcactt caagtgcaaa aagttcttct tgttgtaaac
     7741 tagcgatggg atgtaatgca caaagcgaat aattttcttt tatcacatac tgataccatt
     7801 caggcaattg atcaggcgtg ttttctgtca agccgatata aaaatttcta gtaaaaacat
     7861 tttcaagtaa aaactgccaa tgaccgaaaa agttatcctc tttattgaaa gcctcagaaa
     7921 cagaatgatt tgccggaaca acaaacgcta gatggtcggt ttctgtcatt tctgcaagtt
     7981 ccaatttttc ttggatcatt tttcggctgg aaagtttcat cagctgctga taacctattt
     8041 catcttttgc gtacgcaagc aagccataat ttttttcttc ataagtataa ctaagatgta
     8101 atccaatgat tggctgtact ccttcttcta cacaaatacg atagaattca ggtgcaccgt
     8161 ataagacatc tttatccgtt atggctaatg aagtgtagcc ttgttttttt gcttcttgta
     8221 catattctcg aatacgtatc gtactttgta aaagagaata agaagtaatt gtataaagtt
     8281 gtgggaaaaa catcttgcat tccgcctttt ttttcgttaa aattcactag cgttaaaagg
     8341 tttgatcctt ttactttcat taagatagca ggcgctgaag ttgattattc cacttcattt
     8401 gcttgcttgt aatctgtagg aatgagttct ttcgcaagaa ctcaaatttt attgagattt
     8461 ggtctcgttt ctttacattc aaagaaaatc tggtaaaatt aagatagaaa agaggtgttc
     8521 tttatgcgat atatcgttac attattctgg gctgtcgttc taggtcaagt agtcggttat
     8581 attggtgctg cattaacaag cggaacatat gattttactt tgacaacaat catttcattc
     8641 attgcgggtg tcattatcct tttgattggt gcagttgcgc caagaaaaga aacctctgcc
     8701 cattcgtaaa aaaacagcca agagcttgag atttctcaag ctctttttta ttttctactc
     8761 ctcaccacca ttataatctg ctgcgaagaa ctttacgaaa gttgaataaa gaaagaagaa
     8821 tatttgacag aacaaacaaa cttgtcacaa taaaaacagc actgtaatta taagcatgcg
     8881 cgactgtcga tcctagcatg ggacctaaaa cttgtccaaa gttgctaaac atctgattgt
     8941 agctatagat tcggctgact ccttcttgag gcgtaatctt actgatcagt gtattgacag
     9001 aaggcatcaa agcgccagtt gaaaagccta aaataaagcg aagtatacct aattggagcg
     9061 gtgtagtgac gaatgccata ggtaaataac agcaaaatga taaaatgagt cctccaatta
     9121 gaactttttg attcccaatc ttatccccta gttttcctag tttcggtgaa gaaaagacag
     9181 cggaaacacc agctatcgaa acaatcaagc cgctgacaaa aaggatattt tctgtacttc
     9241 cgctcaattc acgaatatat aaggtcaaaa tagggctgat cgtagtgatt cctatctgta
     9301 aaatcaaggt cgtaatgaag agaccgatca aaatagagag atgatccatt tttgaaaaaa
     9361 tttctttggt gcttatcaga tcttttttct cgattggatg gaaatcttct ttgactaaga
     9421 aaatggtcaa taccgttgtg atcatcaaca aagcccctgt gatcaagaat acattttcca
     9481 taccaaacca ctgagccagt gcaccgccca ttgaaggtcc gatcagattt ccagcaatcg
     9541 ctccggtaga caatgttcct aacgcccatc cgctcttttc tctaggtgcc tgtgaagcaa
     9601 tcatcgctgt ggcatttgga atatatccag acaaaatccc attccaaaac cgcatgatca
     9661 aaagccaata cgcgtttggt acaaaagcca aagatcccat tgttaatgtc atccctgctg
     9721 ctgcacggat catcatgagt tttcggcctt ttcgatcagc tagatttccc cagattggtg
     9781 caaccacggc agatgcaaaa gcagtcacag agattgctag tcccgaaaac aattcgattt
     9841 gatcttttgg tgttcctagt tgctcaacat aaactggtat gaatggcata actaaactaa
     9901 tacttgcacc agtgaaaaaa cagccaatcc acgcaatcac taaatttttt ctccaattaa
     9961 tctccatcta tcatccctct ttcgtctctt ttatcatacc tattttcgaa taaacaagca
    10021 acgatctcgc cagtaaaaag agaaaaatcc cgcaaaggtc attttatatg taacgttctt
    10081 tttatgtaaa acaaaaagag atcagaacaa atactgttcc aatcccttct ctttagttaa
    10141 attgttgcta gaatgatgaa gttgataatg aataaaatat caacgatcca caaagcaaca
    10201 gaaacttgat tgtattttcc tttgattact ttcacaattg tatagaaaat aaatcctgct
    10261 gcaatcccat aggaaatgct atagcacaat cccataaaga tcgatgcaaa gaaggccggt
    10321 aaagcttctt ccatatctag ccatttgatg tctgcaaaag aagccatcat cattactcca
    10381 actaaaatca atgctggcgc agttgcttga tttggtacaa tggcgatcag tggagagaat
    10441 aaactactta atgcgaatag cactgccact actactgaag tcaaaccagt acgtccaccg
    10501 gccccgatcc cagctgcaga ttcgacataa gttgttgtat tcgatgtacc aaagatagcc
    10561 cccacagaag tagcgatcgc atcggcaaat agtgctttat ccatttttgt tttgaaccca
    10621 cgaccatctt ccaacgcgat ctcgtcttct tttgagaaaa tacctgtgcg gcgtcctgta
    10681 ccgataaatg ttccgatcgt atcgaaagta tcagataaac tgaatgcgat gatcgtcatc
    10741 aatacttgag gaatcttgga agaatcactg aataaagatt gcatgccttc agaaccaaat
    10801 gctgcaccga aagttgttcc taattcttta aatgaattac ctaacgaatt cgtttgccaa
    10861 tcaatggaac ttaaatcaac aacacccata aaaataccaa ggactgtggt cgctacgata
    10921 ccgataagaa tagccccacg gacatttttt acaactaaaa tggctgtcag taccaaacca
    10981 atcacagcca ataagatagg agcgttgtca aagttggcta acgcagggac gattccacca
    11041 tttatcgtaa catttgtcgc ttttccgcct tcaacgacag agctcgaaat agctgactgg
    11101 tctgctgaaa aagataagaa tcctgcattt ttgattccga catacgcaac gaaaataccg
    11161 atcccgccac caatcgcatg ttgaagactt tcgggaatcg cccgaataat catttttcga
    11221 atatttgtga cagtaataaa aatattgatt aatccgcaaa taaacaccat tgccaaggct
    11281 tgttgccaag aataaccaag cccaaaaacg accgtaaaag tgaaaaatgc gttcaatccc
    11341 ataccaggtg cctgagcata tggtacattt gcgaacaatc ccattaccag cgtaccaata
    11401 atcgatgcaa tgattgtcgc tagaaatact gcttggaagg gcatacctga tgctgacaaa
    11461 atcgtcggat ttacgaataa gatataactc atggcaaaga atgtcgttac tcccgccatt
    11521 atttcagttg atacatttgt attgtttttc tttaaaccga aaaacttttc cataaaaagt
    11581 tccactccct gaattttttt ctgatggaaa actataataa taaaaaataa gtaaaatagg
    11641 gcaaatccat cgaacaattt caaattatat agacgtttca ttcgcttttc aacgtttttt
    11701 taacaaaaaa agcgaaaata atacttaacg ttcgtgttta gcttatgttt agctatttat
    11761 atagccataa ctgtagtttt tgttaggaat agctgattgc tcttgttaat tacctaaaat
    11821 atgctagact aaaacaaatg ctagttgagg aaggaaggac ccttattgaa taaaaaatta
    11881 attgccatcg atctggacgg aactaccctc aatcaagatt cgatgattac atctaaaaca
    11941 aaagagactc tgaaaaaagc aatcaatgcc ggtcatcatg tcagtattgc tacaggacgc
    12001 ccattccgaa tgagccatca gttttaccaa caattagaat taacaacacc gatggttaat
    12061 tttaacggcg cattagtaca catccctaat cagcactggg atggtgaaag agaaacgttg
    12121 atcaatcgtg aaatcgcttt tgaaatctta tcgcaaaaaa agcagctaaa tttagatttt
    12181 atcgctgctg aaaatcgtga tacttttttt attgacagtt ttgatttctt tgatgaaaaa
    12241 atttttgctt cttctcgtcc aggagagaag aatttgcttt ctcctaaaaa tttgacgaca
    12301 aatcctacat ctttgcttgt ccgaacagac aaacgttttg caggagctgt atctgctgaa
    12361 ttaacacgtc aatttggcag ttatgtcgat gttcggacat ggggtggacc tactgccatc
    12421 ttagagattg tttcaaaagg aatccaaaaa gcaaaaggcg tgcaagagat tgctaactac
    12481 ctgtctatcg atcaaaaaga cgtcattgcc tttggtgacg agcataatga tttggaattg
    12541 ttggattatg caggttgggg agtagctatg gctaacggta ctgaccaact caaaggcatt
    12601 gcaaatgatg tcactccttt gagcaatcag gaagacggtt tagctgttta tctggaaaaa
    12661 ctacttaaac tataatagag aattagattc cacaaaaaag aggttgtgac aatttttgtc
    12721 acaacctttt ttggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
    12781 ataaaaaaat atagaaaaga caccctgaaa tcctttagaa ttaatttggc taccaattca
    12841 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
    12901 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
    12961 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
    13021 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
    13081 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
    13141 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
    13201 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
    13261 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
    13321 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
    13381 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
    13441 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
    13501 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
    13561 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
    13621 gaactttctt gaatcatcga gtgaaagaaa ccacggtacg acttaaagga aattcccact
    13681 ctcaacgagg gttagggaaa aaattgaagc gctattggaa actcctacaa aaagaagaag
    13741 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
    13801 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
    13861 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
    13921 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
    13981 ttctgccaca gatccaaaat gcgcttcact acagctattc aaacggacca ttggaatgct
    14041 taaacaacca catcaaagta ttaaaacgca atgcgtacgg ctttcgcaac ttttacaatt
    14101 tcaaa
//
LOCUS       DASOUD010000065        14033 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.065, whole genome shotgun sequence.
ACCESSION   DASOUD010000065 DASOUD010000000
VERSION     DASOUD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.065"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(47..1366)
                     /locus_tag="U4A29_001828"
     CDS             complement(47..1366)
                     /locus_tag="U4A29_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO0680952.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1363..2016)
                     /locus_tag="U4A29_001829"
     CDS             complement(1363..2016)
                     /locus_tag="U4A29_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0680953.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2304..2824
                     /locus_tag="U4A29_001830"
                     /pseudo
     CDS             2304..2824
                     /locus_tag="U4A29_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2726..3442)
                     /locus_tag="U4A29_001831"
     CDS             complement(2726..3442)
                     /locus_tag="U4A29_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO0680954.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3492..3701)
                     /locus_tag="U4A29_001832"
     CDS             complement(3492..3701)
                     /locus_tag="U4A29_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680955.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3978..5251
                     /locus_tag="U4A29_001833"
                     /pseudo
     CDS             3978..5251
                     /locus_tag="U4A29_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5358..6389)
                     /locus_tag="U4A29_001834"
     CDS             complement(5358..6389)
                     /locus_tag="U4A29_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0680956.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6396..7226)
                     /locus_tag="U4A29_001835"
     CDS             complement(6396..7226)
                     /locus_tag="U4A29_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0680957.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7223..8038)
                     /locus_tag="U4A29_001836"
     CDS             complement(7223..8038)
                     /locus_tag="U4A29_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO0680958.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8035..8859)
                     /locus_tag="U4A29_001837"
     CDS             complement(8035..8859)
                     /locus_tag="U4A29_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEO0680959.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8849..9949)
                     /locus_tag="U4A29_001838"
     CDS             complement(8849..9949)
                     /locus_tag="U4A29_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO0680960.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10127..10912
                     /locus_tag="U4A29_001839"
     CDS             10127..10912
                     /locus_tag="U4A29_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEO0680961.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10945..11328
                     /locus_tag="U4A29_001840"
     CDS             10945..11328
                     /locus_tag="U4A29_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEO0680962.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11404..11535
                     /locus_tag="U4A29_001841"
     CDS             11404..11535
                     /locus_tag="U4A29_001841"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO0680963.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11504..11740
                     /locus_tag="U4A29_001842"
     CDS             11504..11740
                     /locus_tag="U4A29_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO0680964.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11818..12789)
                     /locus_tag="U4A29_001843"
     CDS             complement(11818..12789)
                     /locus_tag="U4A29_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEO0680965.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13423..>14033
                     /locus_tag="U4A29_001844"
     CDS             13423..>14033
                     /locus_tag="U4A29_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HEO0680966.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEK"
BASE COUNT         4273 a         3036 c         2442 g         4282 t
ORIGIN      
        1 taataagtct ctatttttca ataactattg caaaattata tcttaattaa gaaaatattt
       61 ttattttaaa gataaaaaat tcttcatcct gcaatacttt atattctata ttgtaattat
      121 tcagaatatt acttacaata tataaaccta atccattact attttcctta tttaaatcaa
      181 atttaacatc aaatatttta tccatattcg aaattttatt gttaccgtat gaattctcta
      241 tatataacca atcattaact atcccaatat taattacccc atttacatca gtatatttta
      301 ccgcattact aatcaaatta gatagaataa tctttaaagc tgtttttcct atataaacat
      361 tctcgtctaa tatataatta ttgacagtta ttttcttttg atttgctaat atttcatact
      421 tttttaatat gtcttctaat gtatcattta ttttcaaata ttcttcatca ttattcaaat
      481 tttctataga atgaactgat aatatttgag aaatattttt tgttaaacta tcaacgattt
      541 caatacattc attaatataa atatctcttt ctttatattt tccaatattg tatttcatgt
      601 tttccagtat tattttaaga ctagcaagag gggttttaag ttcatgggat gcacctttaa
      661 aaaagtcata ttttaacttt tctaatttta aaatttcttt atttttaaat tccaaatcgt
      721 caattgttct taataaagta gaatacaaat cattaatttg ttgttttaat tctccaactt
      781 catcattaga gctaactttt aaactcattt ttttatcaag tttcatcatt ttatcagtaa
      841 caatttttat ttcttgtata ttatttttta ttgattttgc ataaattaaa gaaataatag
      901 cagaaaataa aattgatatt aataaagaat atggtaaaaa tttaaggctt aaatctttcg
      961 catctttttg catatcagct gtagaaacaa attgtagatt tgttttttta ccatcattaa
     1021 gctttatttc tctttcttca attatcaaag aattactatc actttctaaa ttaacattta
     1081 tattatcttt gatttgtatt tcattcttat tatttttttc ttttatgaat acttttattt
     1141 cactactctt ggaataaaac tccaaagttt gttctatata ttttatttct tttccattca
     1201 tattactaga aatttcattt gccatattat gaatcttttc ttttctcgtc tccaaatatg
     1261 ttttaggaaa gataaaaaaa attaatgaat gaactaatat aattattatt ccaagaacag
     1321 aaaatatttg tatgaacatt tttggaaata tctttaattt tttcattttc tctccaattt
     1381 atatcctaca tttcttatag tagttataca gtctaattgt aatttttttc taagttcttt
     1441 tatatataca tctattactc gatcataagg agtttcttcg ctatctttcc atacataatc
     1501 aataatttgc attcttgtta acacttgtcc attattatcc aataaacatt ttaatacttc
     1561 tagttctttt gcatttacat ctatttcttc accatttatt ttagctgtat agctattaaa
     1621 attaaccgat agatccttat attcaaactt ctctaaatgt ccataattct tcttaattaa
     1681 agaatctatt ctagctttta agaccggcaa tgaaaatggc ttttctacat atccgtctac
     1741 taaattagta aatgcatcaa ttttatattc ttcatcacta aatgcagtca agattagaat
     1801 tggtaaattg cttttctttc taatttcttt caacacttct aaaccattta taaaaggtat
     1861 ctgaatgtct aagataacca aatttatatc gctattaaat tttgacaagg cttctcttcc
     1921 atctttagct tgaataacag tatatccaaa ttctgaaaga tattcactta ttccctctct
     1981 tatcatatta tcatcttcaa cagttaatat tttcatatat acctccactt aagcctatac
     2041 actagatatt atacagcata ttatggctct ataatatcta gtgttggtga cgccttctgt
     2101 tgccaatact ttttcttttt tttaataaat gcaaacagac accttgaaat cctttagaaa
     2161 aaaggcttct ataatatttt gtgttgttgg aaaatcgaag gcgattttct actaacactt
     2221 tttcttcgtt tcaactcaaa aagagactca cttttggtaa aatttagtcg accaaaacat
     2281 caccctacca aaggagtcgc tctatggata atcatactag aaaattactc ggattaacag
     2341 ataaacattt attttttgat gaagaatggc tgattgaaaa agaatataaa ggcgttcaag
     2401 cgcagtttat taaagggaga ttggatgact caccttccca ttgtgaacac tgtggatcga
     2461 ttcgtattat ccgcaacggt tcctatacga cacgtactca gatgttgaaa gtcaaagaaa
     2521 agttgactat tttagaatta aaacgtaccc gttttctatg ctatgattgt gggcagacct
     2581 tttctgcgaa aacggactta gttgatgaac atcatcaact aacaaaggaa ctgaaacagg
     2641 cgattctcat agaactgtac gaaaatcaat cacgtaatct gaaaatcaga agaagtaccg
     2701 tcggctcaag cgatattgga aactcttact caaagattcc acgacattgg agccgctcaa
     2761 acggcattat catcgactgt ttaaacgacc aattagtcaa acagaaatcg tcgatgaacc
     2821 gttgtgcaca atggtgtcct gaacgtacag cgacaccttc catatccaga gcagtcgcca
     2881 catcacgggg atggatgcca tccaaattaa aagcaaccac gccagtatga tgttcaggat
     2941 cttgcggtcc gtaaatggtg ataccgccga tcgcttgtaa tttaggtaaa acgtaggcaa
     3001 ccagttcttg ctcataacga tgaatattct ccataccaat ctcattcaag taatcgattg
     3061 ctcctgcaat gttcggagta ccagcttcaa atttccatgg cagctctttc catgaactat
     3121 cgtaaagatt gacaaaatct atcatttcac cgccgaattc gactggctcc atttgttcta
     3181 acaattgacg tttgccatat aatacaccga ttcctgtcgg cccgcacatt ttatgcccgc
     3241 taaaggcgaa aaaatctgca tccaacgctt gtacatcaac tgtcatatgc ggtgctgctt
     3301 gggcaccgac tggactatcc tcaactacaa cagcctgttc aaccctcagc cgtttcaagc
     3361 acaacaaata accttctgga tctggtttgc cgacctgaag atcatcgatc gtcacaaagg
     3421 aatcaatttt ttccttaatc atcggatgtg cttcaaaaaa atagtctagc tcccgcagtt
     3481 cagaattgag actactccga ttttccagtg ctgctgatcc gctgctcaaa atgttcagca
     3541 tccactggtc tcccaaccgc ttgacttatc ccttcataaa aggacagttc tgctgccgtc
     3601 ccaacacctt gttgatagtc tgcaaaagga attttatagc cgaactgctc cagtaaatag
     3661 gcttcagtaa atagtgatga atactctcgt aaaacatcca ttcactgtca atcatcgtcc
     3721 catcaaaatc aaaaataagt gctttggtca tcttctttcc cccttaccca aaacaagcaa
     3781 aagctgtgtc ctgctctctc tacagtatca gcataaaaac tgccgtctga aaagtcaaag
     3841 gtgaaagaaa tgtggcgcta taatttctag gactgatagt aaatcattct atcagtcctt
     3901 ttgagcataa aaaaacaaaa catacagaag ctccgttaga attgaatcac cacaacacaa
     3961 tcaaaggagc gttctgtatg cctgatcttc atcttatcaa actattacta ggaatcaaag
     4021 atggaaacat tcaaatcact catgtcgaga agaaaaaaat acattcaatc aacagtatcg
     4081 ttgtttctgc cacgctcatt aaaacgattc agccctgtcc tcagtgtaaa caggtgaatc
     4141 aacatgggct gatcgttaag aacggaaaga aaaaatcgct ggtccttctc aataagtgtg
     4201 cgaatcaacg gacttatctt gctttggcaa agcaacggta cctctgtcgt ggctgtcaca
     4261 cctattttac ggcgaatacc tacattgttg aaaaaaactg ttttatcgcc aaacaagtac
     4321 attataaaat attggaggaa ttgactgaaa aacaagcaat gacgacgatt tctaaacatt
     4381 gcggcgtttc ttggtcaacc gtttcgcgaa cactcgcttc tctgctcccc atgacaaacg
     4441 tgaaaataaa ttggttgcct cgctgttcat tggtagatga atttcgttca ttgaaaaatc
     4501 aagtcggtcc ctattctttt tcttgtatgg atggcgatac tgggaaatta ctggatattc
     4561 ttccttcacg taagaagaaa gatctcattt cttattttat gcaatttgaa cgacgtgcac
     4621 gtttaaacgt aaaaatactc gtaacagata tgaacgcctc ttatgctttg ctgataaaag
     4681 agtgctttcc tcatgccaaa ctcgttgtgg atcgctttca catcgtcaag cacctcattc
     4741 gcagttttaa ggatatttga ctatgagtca tgaaaagctt cgaccgaaac gatccaatac
     4801 aagccaaaca ctatcgacaa gtgaaggctt tatctcgact tctgatcaaa cgacaggaca
     4861 cgcttgttta tgataaatgg acaaaatggc gtaattttgg ttgggcctat ttaaccgaaa
     4921 gtgaagtcgt agaacgtcta ctttctactt ctgatgaact acgaatagct tatgcttact
     4981 atcaagaaat tcttcaagct ttttatgaca aagaagcgga catcttcttt cagcttgtaa
     5041 aaacaatgcc caagtcggtt ccaagagaac tgcatcatat aaaaaaagcg tttataaact
     5101 atgaatccgg cattcgttta gccttagagt tgccttactc aaatggcaaa atagaaaatc
     5161 tgcataccca catcaaagca ttgaaacgaa tagcttacgg gttaggatga gaaccagaat
     5221 tttcttaatc aatcatctga tcacttatta aacataaaaa aacatctaaa gcagaaaatc
     5281 aattctacct tagatgttct tatatcactc tatcagtcct atttgacaaa gagccagaaa
     5341 tgtttgccga ctatacacta gctttgttgt gcaaaatgct gagtatcaat ttccacaggg
     5401 attgctagaa tatctttcag ctgaatgtat aaggcatcgc taattttctc ttcccgatcg
     5461 ttcaaccgat ccactaaaat ttctttgccc tccagcgttt gaaaaacata ccgttgaatg
     5521 ttgcctaaaa taaatgattc cttccataga accggaatct taaccccctg aggaaccggt
     5581 tcaaattgaa tcagctccgg tcttaaataa taaatgcagc gattgccctt agcggaaaac
     5641 tcgtgggtat actgattcag ctcataccat tcgaaacgat tatagttgcc gataaattcc
     5701 gcaacaaatc ggctctgagg attctgatac aattccttcg aggtggaaac ctgagccacc
     5761 cgcccagctt ccatcacaaa aatccgatca ctgattcgca tcgcctcttc ttggtcatga
     5821 gtcacaaaaa tcatcgtcat ccctagcgat tgctggatag cgcgcaaatc ccgctgcagc
     5881 tgttttctaa ttcgggcatc caaagcactt aatggctcat ccagcagcag gactttcggt
     5941 tcagtgacca atgagcgagc cagtgccacc cgttgtcgtt gaccgccaga aagaaactgg
     6001 acatttttat ctgcctgctc cgtcatatcc accaatgata gcatctcaaa aaccttttcc
     6061 tgaatcacct caggactcag cttctttacc ttcaaaccaa aggctacatt ttcaaaaaca
     6121 gtcatagttg gaaacaatgc atactgttga aaaacaaagc cgatattgcg atccttcgta
     6181 ggcttatcgg caatattctc attgtccagc acaatctcac cgctctttgg ttgttccaat
     6241 cccgtgatac agcgcagcaa cgtagacttt ccgcacccgc tgggacctag taaagtaacc
     6301 agctcaccct tttcaatctg aatttcatca atagctaaaa tctgtttttc atcaaaaaac
     6361 agattgagat catgtatttc taaaaacatt ttccgtcaca actcctttaa gggcttcttt
     6421 ttcgactgct tctcttccct cgtctgctta agggacagct gttgcttttg tcgataagtc
     6481 agcataaagg tagttaaagc aattcccacc ataaacagca aatacaaaac catcacagcg
     6541 ctagccaaat ggccgttttc gttcatccgc cgcagcatat agatgcgtac cgtctgaaat
     6601 cgaccgccaa tcaacaggtt cgtcagcatg tactccccaa aaaccgaaga aaaaatcatc
     6661 agagaaacta gggtcacacc gatacggatg tttggaaaca gcacctggat aataatcgta
     6721 gacatcgggg cgcccaaggt ttctgctgca tccactaatt ccttcagatt aattaagcgc
     6781 atctggttgt taagactaag atagacaatc ggcaacgccg taataaacaa tgcacctacc
     6841 aacactacaa acattgggat accagtttca gcatacgttc gaagcaaagc cgttaccaaa
     6901 atcactcctg gtaaagcata cggcagcagg agcagctttt ccagccaacg attcaaacga
     6961 gggaaataca aatgaatcca aataatggtg ggaatcatca acaacagaat cgtcaccaag
     7021 accacacctg ctaataaaag tgagcgaccc accgcagcaa taaattccct gtccgtcagc
     7081 agctgttgga accattgcaa ggtaaaatca ctaggtaaaa gcgattttga ccatttagtg
     7141 gagctggcat acaaaatcgt tccaataatc ggcagcaagt aatacaggac aaagcagaac
     7201 aaaatcggtt ttattaactt tttcacgtct gcaccacctt gcctttcttt tgaataatca
     7261 acattgccgt taccagcagg acacctaaag cgactgccaa cgcactgcct aaattaggct
     7321 tggcgaacac atctcccgac actaaagccg caattcgaat cgttaaaaga tttacattac
     7381 tgctggtcag ggcatacgct gtttcatagg tccccatgcc attagcaaat aagatgacaa
     7441 aggtgctggc aatcgatggt cgcaaaaaag gcaaagccac cttgaaccaa gcatacgctg
     7501 atgaagcgcc cagcaattgt gcggactcca gccattcctt tttcacttcc ttcatgctgg
     7561 gatacaaaaa caagacccct aacggaattt ggaaataggt atacgtgatg gtcaatcccc
     7621 accaagaata caaattgaag tccgctagaa atggcaaaat cattttcagc acacctacat
     7681 tccccaacaa aataataaaa gagaatgcca gcggaatccc agcaaaatta gctgccaaat
     7741 tggaaacaaa ggtgattttc tcctgcaatt tagtcgatag attcattaag cataaacata
     7801 aaataaacgt cccgattaac ccaatcacac tggagctgac agcaatcagt acgctgttaa
     7861 caaaggcctg tgtgtaatat ttgttgctga aaatatcact gtaattggcc aatgtccact
     7921 ggttagttaa atcactgtgc aagctgcaca gaaccataaa aagcagcggc agcagtaaaa
     7981 atgcacagaa gataaaggag gcaggaacca gaaccaagcg ttttttcatc tctttcatcc
     8041 cctcatcaat tcttgaatgt cctgcatttt ctctgacggt gcaattccta ataaccagca
     8101 aaacaatgga gccgccagca gctgtggtac agtttgttcc ttcagctttt ggcgtttatt
     8161 tgaaatcaaa taaaaaggaa cttcacgatg tgcagccaag ctgccgccat gcagtccgtg
     8221 cgcatccatc ccgtgatccg cagtcaccac gacttgataa cctttttgca gccattcagg
     8281 aaggtaaata gccaataatc tgtccgcctg attaaccgct gctgcatatt catgagaatc
     8341 ggcggtaaat ttgtgaccga catcatcaat attcatcgaa tgtacgacca aaaagtctgg
     8401 ctgatactga ttcactaaat aatgggcatc cgcaaataaa tgcgaatcag ggtaagtgtc
     8461 ctcactataa aagatgccat gctgaatcag tcgctcctcc tgatgctgga tgcgatcaac
     8521 caaacgatta tagggggctt gattgtacaa ctcgctgtac cagtggtaag cggcggctcc
     8581 tgtttttccg ccagcttcct taactaattc aaacagcgac cactctttgg atcgctgagc
     8641 atggtaatta ctatagatgc cattttcata ggctggaacg ccggtcatca gtacttcgta
     8701 taacggacgt gaattgctgg gcagctcact ttttaccttc aataagcttg cttgctggtg
     8761 ctcaactaaa tgattcatga acccaagctg ggcgacggct gtgtcgtagc gacagccgtc
     8821 tagtataact acaatcagtt tgtcaagtct actttgcata ggtcagtacc tccgcctgcc
     8881 aaagctctgg aagctctaca gaggtcttgt tccaagcttc attgtcttca actggcaccg
     8941 ccgcttcata agcatcctcc ggcagtaatt tttcagctac ttctttaggc agctctacct
     9001 tgcgaatagg tcgcgcatag ccctttgcca aattgatttg cccttcatca gataaaatat
     9061 attcccgtgc tagctttgcc gcattgggat gaggggcatt tttattgatt aaggttgtat
     9121 acccgctaat gatggagccg tctgttggca cagttacctc aaaacgctcg gagtcaattt
     9181 ggtcacgata atttagtcca ttgaagtccc aaacaactgc cacatcgatt tcaccttttt
     9241 caagatttgc tacgctggca tcaacggtgg acaaacggcc tttttctgcc atttttttgt
     9301 agtaattcag acctggctga atatcctttt cgtcacctcc gttggcaatc gccgcagcca
     9361 atacagcaaa ttgtgcttgg ttggcaatcg tcacatcccc cacgctaact tgatagtcac
     9421 cctcttgcag ctccttccag ctagtcggtg cttgcttcac attctcttta tcggtgataa
     9481 aggaaatggt tcccgtatag cttaacaacc aatccccatc atcatccttt gcccaatccg
     9541 gaatttcctc ccaatagttg gtcttataag gtaaggttaa ctctttgctt tcagccaacg
     9601 gaccgaagct gataccgaca tcaccaatat cggcggttcc attttttcct tctgattcaa
     9661 acttagccaa ttcttctgca ctggacatat ccgtatccac gtgacttaag ccgtattctt
     9721 tttcaaggtc gttccaagtg cctacccaat ttgcccaagt atccggcatt cccaagctgg
     9781 caatctctcc ttcttccttg gctttttcta taatcgtatc taaagagtcg tctgccgcag
     9841 ccgattgctt cgttgcttca cctgagccgc agcctgtcag caggatactt actgttgctg
     9901 cacaaaataa accgatagtc atgttacgaa ttttttcatt cattttcatt cattcttctc
     9961 ccctttcgat aattccattc taacaaagct ttgtaatgag agaatcagtt tcttgaaaag
    10021 gtttcatgaa caatgtaaag gttgtatatg gtttgtaaat ttcgacgaat tcccgttaac
    10081 cttctaaagc ttttgcggta tgatagtaga aaaaaggagg ctccctatgc agtattatga
    10141 tcagcatctc cacacgtatt tttcctttga ttcagaggaa aaatttgaaa actatttagc
    10201 ctatcagccg gagtttttcg tttctacgga tcactttgat ttaaacaatc catctaccgg
    10261 aaaagatgac attccggatt atccagccta tataaacaaa ctggactctt taaaagaaac
    10321 ctattccacc aaatttctgc gaggtatcga gattggcgtt gttccaggac aagaagagcg
    10381 gatcgtggat tacctcgctc agcatcctta tgatttaaag ctggtaagta ttcatcaaaa
    10441 tggccgcttt gattatatgg acgatgtggt tttaaccaaa gatcccattc aaactaccaa
    10501 ggaatatttt gatcagatgt tgcaggtctt gacggatttt ccagaaggag atattttgac
    10561 tcactttgat tatggattac gccgcttcac cttcacagcg gaagaattcg cggagcattt
    10621 tgaggctcag ctgctaacga ttttcaaaaa ggttgttgaa cgagaattgg cgatggagtt
    10681 aaatgccaag agcttcttga aatacggcaa cgaagacctt taccgttatg ccgttccttt
    10741 gtatcaaagt gtcggcggaa agctctttac cttaggctcg gatgctcatg ttgccgaaga
    10801 ttatcagctg ggctttaaaa aaatgagtca gctgttggca gactgtcagg tagaaaagtt
    10861 gctagtcatt caaggaaaag aacgccgcct gacgccgctg ccgattttgt gattcctata
    10921 taatagaaga aagtggtgaa aaaaatgagc aacctgtggg actacaatca agaggcaccc
    10981 atccattatt taattgcgcg acattgggat gcgttgaaaa ttgaggcggt ttgccgatcc
    11041 ttgttagcag ccgttccaaa acaacaactg gaaaattttt tggtggctga cagcttgcag
    11101 cgggaaaaag ttcaggctta ttttgcagcc tttaaagatc agcctttgga atatttgcat
    11161 gcgcagtttc atctttttta tcaagtagcc gccccagatg actacaacga tttacgagga
    11221 cagctgcaat tgacctttca agcggatgaa accgcctata ccgttttatt gggaatggct
    11281 cgccttggtg atcaggcgaa ggtcgagtgg cggattttcg atatttgatg gcaggatcaa
    11341 ggcgtttcag ttgcaaacct tcctctcttt caattagaaa aaccgcagca ggaagaagtt
    11401 tgtgtgctgg tcaccgatgc tggtgaaatt cgtctccgct tatttcctga ccaagctccc
    11461 ctggctgtag ccaactggcg ctccctagct gagcaaggcg tttatgacaa cacacctttc
    11521 ggctcgtgtc attaaggaat ttgttattca aggtggcgct ttggatggtt ctggtgatga
    11581 agctgtttcc agctatgagg gctttttcgc tgatgaagtg catcgcggac tttatcattt
    11641 taatggcgcc ttggccttag gggaccacgg cccccacacc aacggcaacc agttttttat
    11701 tgtgcaaaac acaaaggcgc aggctgacct gctaatgtaa agcaagcata cttggagctt
    11761 ggaggactac cggagctgga tggtcgttat actgtttttg gacaggtata ccaagggtta
    11821 gaaatcgtag cagcgatcgc agctcagccg acaaacagtg aagatgtacc gattgagcca
    11881 gtgttcattc gacaaattaa cttcgaaaaa acatcgtaaa aagacactca ctgacttcag
    11941 caaaagctct attcttgagc tggctgcagc tagtgagtgt ctttcattta tccataagat
    12001 aaagcttgga ttcaataaat aaataaatgt tttggttaaa gctgtgacca gcaaatgagg
    12061 aaccgtgcga gccagttgat ctgccaatgc aaacatttct ttaggtgaag gcgctggcgt
    12121 gtctgccaac ggatactcca gtcccaatgc ttggtaaccg tgcataccat agcagtggta
    12181 cggtagtaat tctaacggta cgaaccgatc aagatctgcc acaaaacaag caatcgtcag
    12241 cagctcggtc aggttcatat tgatacctgg aaccacagga actcgaattc gcacttcgca
    12301 gttaggatac tcaattaaaa agcgacgaat attcttcaaa atttgttggt tatctaagcc
    12361 ggtccattgt ttgtggagct tgctggtaaa catttttaca tccacaaaca atgtatccac
    12421 atagggagct accttttgga tttcattata agcaccacaa aagctggttt ccagtacggt
    12481 attgattcca atgtatttcg atttttgcaa aatttcttta gcaaaatccg cctgaaccag
    12541 tgcctcgcct ccgctaatgg tcactccgcc atccgagtga aagtaaaaaa cagcatcctt
    12601 ctcaatctca tccattacct cttcagcggt catggtctgg ccaaatcctt tttcaatctg
    12661 cttgctttgt gattcgggaa tcgaacacca ggcacagcgt aatgggcagc cctttaaata
    12721 aacaacggtc cgtaaaccag caccatcatg tagagaaccc tgttctattc ttaatactgt
    12781 tccttccata tagatttttg aattacacac tagtttaaac taaaacacac aaggaaaaac
    12841 ttaccattta atcataaaat tagctcatat aaatatacta ggatgtaaaa tagactggtc
    12901 aaaatctggt caaaaaagag gactaataca ggtagtcctc ttttttgtta ttttaatatt
    12961 ctaaatcatt ttttattggt ttattccctt attcgatgta gactcaaatc actatatcac
    13021 tagatatcaa acggcgtcat acggaatcaa aaacatcaaa tttgacctgt taaatggtca
    13081 ttgaataaca ttcaaaatca ataaaacgga gtcagacacg gagtcaaaaa cgctgagagt
    13141 aaaaaatata tacagccttg taggtaaagt accacagggc tgtatatttt ttatttcaga
    13201 ttaccttaaa tgattccatt tactttttat ctcatttttt cctactagtt agtatcaaat
    13261 actctcctat tttctcaata aactattttt ggatacatgg tatctgaaac agctaaaacc
    13321 ttgtcattac agcaaaaaat cacgttatcc agactgtaga caaagtccat tttatggatg
    13381 atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg agtgatagct
    13441 atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac gattgatgat
    13501 ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt tgaatttatt
    13561 tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga tccagtgatt
    13621 ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg acaaaccatt
    13681 aaggaagtcg ataccaatgt cgcttaccgc tggtttttag gctactcctt cgaggagaaa
    13741 atcccccatt tctcaacttt tggaaagaat tatgttcgcc gatttagaga aacgacggtg
    13801 tttgaggata tttttgccta tattttagaa caagcagtta aagcaggttt tgtaacagag
    13861 gataatttgt atctggattc tacccacatc aaagctaacg ccaataaaca taagtttacg
    13921 aaagaaatga cccatgatga agcgaaagcc tatcaggatg aattagaaga tgagatcaat
    13981 cgtcaacgaa tagaggctgg aaaacgccct tttactttgg acttagaaaa gga
//
LOCUS       DASOUD010000066        13918 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.066, whole genome shotgun sequence.
ACCESSION   DASOUD010000066 DASOUD010000000
VERSION     DASOUD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.066"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U4A29_001845"
     CDS             131..505
                     /locus_tag="U4A29_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEO0680967.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1242)
                     /locus_tag="U4A29_001846"
     CDS             complement(619..1242)
                     /locus_tag="U4A29_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO0680968.1"
                     /translation="MFFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTATL
                     DDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNRLL
                     LTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTKDH
                     ANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="U4A29_001847"
     tRNA            complement(1383..1455)
                     /locus_tag="U4A29_001847"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
     gene            1669..1742
                     /locus_tag="U4A29_001848"
     tRNA            1669..1742
                     /locus_tag="U4A29_001848"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1703..1705,aa:Asn,seq:gtt)
     gene            complement(1880..2044)
                     /locus_tag="U4A29_001849"
     CDS             complement(1880..2044)
                     /locus_tag="U4A29_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680969.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2339..3667
                     /locus_tag="U4A29_001850"
     CDS             2339..3667
                     /locus_tag="U4A29_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEO0680970.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3869..4702
                     /locus_tag="U4A29_001851"
     CDS             3869..4702
                     /locus_tag="U4A29_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEO0680971.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4718..5455
                     /locus_tag="U4A29_001852"
     CDS             4718..5455
                     /locus_tag="U4A29_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO0680972.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5610..6578)
                     /locus_tag="U4A29_001853"
     CDS             complement(5610..6578)
                     /locus_tag="U4A29_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO0680973.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="U4A29_001854"
     CDS             6810..7643
                     /locus_tag="U4A29_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEO0680974.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="U4A29_001855"
     CDS             7677..8516
                     /locus_tag="U4A29_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO0680975.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="U4A29_001856"
     CDS             8542..9102
                     /locus_tag="U4A29_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO0680976.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="U4A29_001857"
     CDS             9301..11022
                     /locus_tag="U4A29_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO0680977.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11187..12329
                     /locus_tag="U4A29_001858"
     CDS             11187..12329
                     /locus_tag="U4A29_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO0680978.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12345..13556
                     /gene="thiI"
                     /locus_tag="U4A29_001859"
     CDS             12345..13556
                     /gene="thiI"
                     /locus_tag="U4A29_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO0680979.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4752 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tacttgattc cggcatagct
     1681 cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactgccgg
     1741 agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta
     1801 ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag
     1861 tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca
     1921 gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt
     1981 tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt
     2041 tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg agtattttga
     2101 aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg
     2161 ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc
     2221 ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta
     2281 tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat
     2341 gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa
     2401 acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt
     2461 ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc
     2521 taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa
     2581 gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg
     2641 ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc
     2701 aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg
     2761 ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga
     2821 aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa
     2881 agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga
     2941 aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc
     3001 tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg
     3061 actgacacca caaatttttt atgataaata catcggtgta aaattagatg attatgttag
     3121 cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct
     3181 aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa
     3241 aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg
     3301 tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct
     3361 atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat
     3421 gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa
     3481 agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc
     3541 ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca
     3601 attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt
     3661 agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc
     3721 cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct
     3781 aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag
     3841 aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa
     3901 attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa
     3961 aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga
     4021 agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat
     4081 gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg
     4141 ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt
     4201 tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga
     4261 ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac
     4321 gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa
     4381 atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa
     4441 tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa
     4501 tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga
     4561 tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca
     4621 gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga
     4681 tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa
     4741 gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt
     4801 aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt
     4861 cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct
     4921 ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt
     4981 ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc
     5041 tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat
     5101 tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat
     5161 tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca
     5221 tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac
     5281 tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga
     5341 gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat
     5401 ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag
     5461 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
     5521 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
     5581 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
     5641 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
     5701 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
     5761 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
     5821 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
     5881 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
     5941 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
     6001 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
     6061 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
     6121 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
     6181 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
     6241 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
     6301 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
     6361 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
     6421 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     6481 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     6541 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
    11041 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
    11101 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
    11161 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
    11221 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
    11281 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
    11341 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
    11401 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
    11461 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
    11521 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
    11581 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
    11641 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
    11701 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    11761 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    11821 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    11881 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    11941 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    12001 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    12061 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    12121 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    12181 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    12241 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    12301 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    12361 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    12421 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    12481 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    12541 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    12601 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    12661 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    12721 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    12781 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    12841 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    12901 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    12961 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    13021 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    13081 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    13141 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    13201 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    13261 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    13321 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    13381 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    13441 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    13501 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    13561 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    13621 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    13681 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    13741 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    13801 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    13861 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttg
//
LOCUS       DASOUD010000067        13836 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.067, whole genome shotgun sequence.
ACCESSION   DASOUD010000067 DASOUD010000000
VERSION     DASOUD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.067"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            168..362
                     /locus_tag="U4A29_001860"
     CDS             168..362
                     /locus_tag="U4A29_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680980.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="U4A29_001861"
     CDS             631..930
                     /locus_tag="U4A29_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680981.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="U4A29_001862"
     CDS             complement(1027..1461)
                     /locus_tag="U4A29_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0680982.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="U4A29_001863"
     CDS             complement(1693..2403)
                     /locus_tag="U4A29_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO0680983.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="U4A29_001864"
     CDS             complement(2393..3253)
                     /locus_tag="U4A29_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO0680984.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="U4A29_001865"
     CDS             complement(3258..3896)
                     /locus_tag="U4A29_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO0680985.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="U4A29_001866"
     CDS             complement(3911..4210)
                     /locus_tag="U4A29_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0680986.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="U4A29_001867"
     CDS             complement(4253..5737)
                     /locus_tag="U4A29_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO0680987.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="U4A29_001868"
     CDS             complement(5757..6218)
                     /locus_tag="U4A29_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO0680988.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="U4A29_001869"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="U4A29_001869"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO0680989.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="U4A29_001870"
     CDS             7521..8291
                     /locus_tag="U4A29_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO0680990.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="U4A29_001871"
     CDS             complement(8564..9391)
                     /locus_tag="U4A29_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0680991.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="U4A29_001872"
     CDS             9959..11194
                     /locus_tag="U4A29_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEO0680992.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="U4A29_001873"
     CDS             complement(12193..12453)
                     /locus_tag="U4A29_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO0680993.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="U4A29_001874"
     CDS             complement(12480..13781)
                     /locus_tag="U4A29_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0680994.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4453 a         2832 c         2177 g         4374 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaag
//
LOCUS       DASOUD010000068        13800 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.068, whole genome shotgun sequence.
ACCESSION   DASOUD010000068 DASOUD010000000
VERSION     DASOUD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.068"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..362
                     /locus_tag="U4A29_001875"
     CDS             12..362
                     /locus_tag="U4A29_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEO0680995.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            343..1059
                     /locus_tag="U4A29_001876"
     CDS             343..1059
                     /locus_tag="U4A29_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0680996.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            1059..2507
                     /locus_tag="U4A29_001877"
     CDS             1059..2507
                     /locus_tag="U4A29_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO0680997.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2577..3086)
                     /locus_tag="U4A29_001878"
     CDS             complement(2577..3086)
                     /locus_tag="U4A29_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680998.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(3076..3801)
                     /locus_tag="U4A29_001879"
     CDS             complement(3076..3801)
                     /locus_tag="U4A29_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0680999.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(4024..6144)
                     /locus_tag="U4A29_001880"
     CDS             complement(4024..6144)
                     /locus_tag="U4A29_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO0681000.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            6374..7552
                     /locus_tag="U4A29_001881"
     CDS             6374..7552
                     /locus_tag="U4A29_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO0681001.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            7568..8326
                     /gene="aroD"
                     /locus_tag="U4A29_001882"
     CDS             7568..8326
                     /gene="aroD"
                     /locus_tag="U4A29_001882"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO0681002.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            8349..8834
                     /gene="ybaK"
                     /locus_tag="U4A29_001883"
     CDS             8349..8834
                     /gene="ybaK"
                     /locus_tag="U4A29_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEO0681003.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(8905..10158)
                     /locus_tag="U4A29_001884"
     CDS             complement(8905..10158)
                     /locus_tag="U4A29_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO0681004.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            10275..11624
                     /gene="gdhA"
                     /locus_tag="U4A29_001885"
     CDS             10275..11624
                     /gene="gdhA"
                     /locus_tag="U4A29_001885"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO0681005.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            11736..13082
                     /locus_tag="U4A29_001886"
     CDS             11736..13082
                     /locus_tag="U4A29_001886"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO0681006.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(13390..13776)
                     /locus_tag="U4A29_001887"
     CDS             complement(13390..13776)
                     /locus_tag="U4A29_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEO0681007.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4530 a         2452 c         2828 g         3990 t
ORIGIN      
        1 ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt
       61 ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg
      121 caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta
      181 tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt
      241 ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa
      301 agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt
      361 gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa
      421 gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat
      481 tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga
      541 gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa
      601 agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt
      661 cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca
      721 agagaagaag cagtagtttc agatgatcat tttgatatcg agactagtca tttcaagatg
      781 aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa
      841 gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag
      901 ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat
      961 atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg
     1021 ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac
     1081 tcttagcttt ctgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac
     1141 aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat
     1201 cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg
     1261 cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta
     1321 tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta
     1381 ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga
     1441 ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga
     1501 atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg
     1561 ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta
     1621 ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga
     1681 cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat
     1741 ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag
     1801 aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga
     1861 gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg
     1921 aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt
     1981 tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta
     2041 aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag
     2101 aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt
     2161 atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa
     2221 acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag
     2281 atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag
     2341 tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc
     2401 agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa
     2461 caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta
     2521 taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag
     2581 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     2641 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     2701 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     2761 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     2821 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     2881 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     2941 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3001 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     3061 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     3121 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     3181 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     3241 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     3301 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     3361 aactgactgg aagcttacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     3421 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggtgcca
     3481 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     3541 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     3601 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     3661 attggctaaa gccgattgca aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     3721 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt
     3781 agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga
     3841 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
     3901 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
     3961 tgtttattcg aaaagaggag cagcgacaat aattttgtcg ctgctccttt taaatttatt
     4021 ttatcattcg gaagaacttg tggtcgttcc gtgaagttct tggtacgttt gcgttttata
     4081 aagatcaaca gttgattgtc cgttgtgttc actaaagaca ctagttgact tgtcaccaag
     4141 agatttttca gcttctttta atttctctaa gccattcttg tagtcataat cactggtatc
     4201 aattgggtct aatccactat tcgtatagaa ccgaagaaga tcaccattat tgatttggtc
     4261 agaaacagct aactgtttat caactttttc tttcagtgaa tcaatttcat tctgtacttc
     4321 ctcagtaggt tcagtaatag cttcaccagt ttcgttacta tagatcgtac caccgtagta
     4381 agtatatttt ggtgtaacaa aatcaccatc acggaaagaa acaagctgtt catgatcttt
     4441 cgagaataga tcttgtccta actgaatata attttttgta tctacaccta gtaagtgaag
     4501 taatgtaggc aaggcatcaa cttgtccgcc gtaagtgtga ttgattccgc cctgtgtttg
     4561 tccagggata tggatcatat aagggacacg ctgcatttgt gcattgtcgt agtttgtcca
     4621 agtagaactt gtttttccaa ctaattcagc tagattttta tttcttgaat tcgagacacc
     4681 gtaatggtca ccgtaaagga cgatgactga attatcatac aagccgcttt ctttcaaata
     4741 attgaagaat tcttgtacgg cggtatctaa ataattggct gtcgcaaagt aaccgttgat
     4801 cgtttcatca tttgtattgg ctaacgggaa tcctgcttcg tcattggtga attttgaata
     4861 aggataatgg ttagatacag caatgaattt tgaataaaat ggttgttgca gacgttccaa
     4921 gtattgtacg gattgttgga aaaatggttt atcatgcaac ccgtattgga acgaattgtc
     4981 atcattaaca tcataataag aagaatcaaa gaaataatca tatcctaaat tcttgtatgt
     5041 ctcgtttcga ttccagaatg taccagcgtt cccatggaat accgcactaa tgtaaccttg
     5101 tgtttgactt aaaatatcag gtgcagcttg gaaagtattt tttccgccta attgtgtgaa
     5161 caaggagcct tgattcaagc caaacaatga attttccatc agcgtttccg catcgcttgt
     5221 ttttcctgct tttacttgat ggaaaaagtt gtcaaaactg aacgtgctgt tgctgtggta
     5281 aagactattg atgaaaggtg taacttcatg ctggagacca ttctcatctg tcaatttata
     5341 atcgatcagg aattgctgga agctttctaa atggatataa atcacatttt ttcctctagc
     5401 gagcccgaac aaatcgtcat ttggtgctgc ataatgttct tttacataat tttctacttc
     5461 tttcatatca ttgggacttg cttctgcccg cacttggttt gtctggtaag tttgaacacc
     5521 gtcataaacc atgaaagcat tcaagccaag atatttgacc aaataatcac gagagaatgt
     5581 tcgagtcagc aattctggac gatcagcttc cgccataaaa agatttcctg agaaaatcat
     5641 aactgctaat gtagagatcg caaaagccat tcttgctcgg atcgggcgag ggtccaactt
     5701 gatttttttt gcaatcagca gaacagtcaa gataataaaa tcgatccaat agatcacatc
     5761 atatgggcga aacagtcgaa tggcactttc tcccagacca ctcgctactt tcccagcacc
     5821 taaaatcgtg ttgatcgtaa tgaagtccgt gaattctcga taataagtga cattcgaaaa
     5881 aacaagcaat gtcaaaagaa aataaatgac catcatcgtg atataagcaa ctctagggcg
     5941 tctgatataa agagcaattc caagtaataa caatgtagtt gcaatagggt tgataaacaa
     6001 gataaagtat tgaatgccac tttcgattcc gagatggaaa tcaactaaat aggcaaaaat
     6061 cgttttcaac caaagtaaaa cgactagtaa agaaaaaaat ccgagcctct tattcaaaaa
     6121 ggcaggtgtt tttatacatt tcaagataat cttcctttct gcacttctta atcacagaag
     6181 tgtcctgaaa ccatgttaca cagctcactt attctcattt attttactag tatgacaata
     6241 tagtgtcaat tgaaaccaga agtttatgct gtttcattaa ggttttcttt aagcttcatg
     6301 aaaaaatgag cagtaagaca agtcctatgt tttttgttat acttgggaag agaaagctaa
     6361 agcaggtgaa ggaatgaaag taaaagtaaa aaaaagagca agcaatcgat tgaaaaaagg
     6421 atatccactg atccaaaaag aagaccttgc tcatccccaa aaagaaacca cgcaatgggt
     6481 ggaattagtt gattcgcaaa accaatttat cgcaaaagga tatctaggaa agcagaataa
     6541 gggaatcggt tgggtattga gccagcagaa catcccattt gatcaatctt tttttgaaga
     6601 attttttgtg aagtcaaaag aaaaaagaac tttctttttt tcagatgatt ctactaccgc
     6661 ttttcgtata ttcaatggag aaggagacgg gatcggcggt ctgattgttg attattatgc
     6721 aggttatgct gttttttctt ggtataacga aacgttatat gcacatcgtg aattattgat
     6781 tcaagctttt cgtgaaatct acccggaaat cataggaggc tatgagaaaa ttcgatttga
     6841 atcagtaaat ctgcctgaga gccagttcat ttacggaaaa gaggcaccgg aacccttgat
     6901 cgtaaaagaa aacggagtaa aatttgccgt ttatttaaat gaaggattaa tgactgggat
     6961 ctttttagac caaaaagaag tacggggatc attagcagaa ggtttcgctt ctgggaaaac
     7021 agttttgaac atgtttagtt ataccggcgc cttttctgtt gctgccgcaa tgggaggggc
     7081 aatcgaaaca acaagtgtcg atctagcaaa acgcagttta aaaaaaacac aggaacagtt
     7141 tgaagtgaat ggtttggatg tcgatcagca aaaaatcatt gttatggacg tattcgagta
     7201 tttcaaatac gccaaaagaa aacaattttt atatgacatg attgtattgg atccaccaag
     7261 ttttgccaga aataagaaaa aagtctttcg cgtagcaaaa gattatgggg aactggtcaa
     7321 ggatagtctg gatattttag cagaagacgg gatacttatt gcgtctacaa atgctgcgaa
     7381 tgtctcgata gaacaattcc aaacaatgat tgaagaggaa ttccatgcca aaaatgtttc
     7441 ttttgaacaa cttgctttat atcgtctgcc aaaagatttc cagacgacgg ataccttttt
     7501 agaaggcaat tatctgaaag tgtttgttta tcaaattaaa aataaccatt agaaggagcg
     7561 atgcagaatg gttcgggtaa agggattgaa tataggaagc ggtagaccaa agatttgtgc
     7621 gcctcttatc ggggccgtcc gagaggaagt attacgtgaa gcagttcttg cacgtgaagc
     7681 aggggccgat ttagtcgaat ggcgagtcga tcattatcgt gaagtttttt cttttgatga
     7741 agtagcagaa acattggttc ttatccatga gatgcttgat ggactgccgt tattatttac
     7801 gtttcgtacc ttagctgaag gcggagaaca agacatatcg atccgtcagt atcgcgacct
     7861 ttatgaacat gtgatacaca caggattagt agatatggtc gatatcgagt tgttcaaagt
     7921 agaaagtcta ggaaaaagac tgatagagga aatcaagtct ttaaacattc ctttaattat
     7981 cagtagtcat gattttaaag aaacaccagc agaccctgtc ttgctttacc gattgaatgt
     8041 tatggaacat tttggagccg atatcggaaa actggcagta actcccaata atgaacgaga
     8101 tgttttgcgg ttgatggagt tgactagacg ggcaaatgct tttgtctcca tgcctattat
     8161 tacgatgtcc atgggaaatc ttggtaaaat ttctcgtctg gttggcggaa cgacgggttc
     8221 cgttatgaca tttggttcct taagcagcga ttctacttct gctccaggac aattaccggt
     8281 caaagcattg aagcaaatta ttcaattatt aaatgaaccg ttgtagaaca agtttgaggt
     8341 gatatttttt ggcaaagaaa aaaatagtaa aaacgaatgc aatccgtatg gtcgaacaaa
     8401 aaaagattcc ctatacagag cacgaatatg agtgggacga aagccaccta agtgcgtcat
     8461 cggtcgctga acagcttcca gaaagccaat ctcgtatatt taaaacactg gttgctgtgg
     8521 gcaatgtgac ggggcctctt gttgctgtga taccgggaga agccgaattg aatctaaaaa
     8581 aattagcaaa agtttcaggg aataaaaaag tagaaatgct ccatttaaaa gatttggaag
     8641 caacgaccgg atacatacgt ggtggctgct cgcctatagg tatgaaaaaa ctatttccta
     8701 cttatttaga tcaaattgca gaaagctatg aacaaatcat tgtctcggct ggcagaagag
     8761 ggctacagat ggagttagca cctcaagata tctgtgcatt aacgagcgga caatttgcag
     8821 atataaaaca ataaatcata cgtataaaag aaagaaggag gccgggatgt tgtttgtcca
     8881 gtctccttct ttctttttta tacattatgc tctttgcccg gcattcattc ctgctacatg
     8941 ccctgtagcg aaagccgctg tgatattaaa gccgccagta taaccattga catccagtaa
     9001 ttctcccgca aaatacagac catcaatgat tttactctcc aatgttttag gataaatttc
     9061 ctttagagaa acccctccgc cagttacaaa tgatttctca ataggaaaag ttttgttgat
     9121 tgatagctta aactctttgc atagttgaac aaaatcttga atttgttttt gagaggcttg
     9181 ttgtcctgtc agatgatcca tttcaagccg ctcaagaaag aaaactaaca atcgctcagg
     9241 caatatacca tgccaagcat tgaccaagtt ctttttggtt gctttacttt tttctgtcaa
     9301 tatatgaata agctcttgtt tggtttgtgt agggaaacaa tccagagaaa gtgtcactgg
     9361 ttctcccgtt ttctctaatt ctttattgac aaaacttgaa caacgcagtg cagccggacc
     9421 agaaataccg aaatgggtga aaagtaaatc catcgtatgt tcagtcaata cacgaccttt
     9481 tttatttaaa acgcgtaaag tgatgtcttg taaagaaagc ccttgaagtg ttttctcctg
     9541 aatatacggt tcatccgata tcaaggggga ctctgtcgca taaagaggcg tcaccgtgtg
     9601 acccactcgc ttcacgatct tgtacccatc tccagtagaa ccagtagatg gataagtccg
     9661 tccaccagtt gttaagataa tggcaggagc atggaactct tcgaattccg tgcgtatacc
     9721 gtaaatttta tgttctttgt ggatcaattt ctctactcga gtagagaata gaagcgtcac
     9781 atctagttct ttcagacgat tgagcaatgc ttcgataatc gtttttgatt tatttgttac
     9841 aggaaacatc cgtccatgat cctcttcttt taaatgtacg ccttgtgatt caaagaagtt
     9901 catgatatcg aaattattcc attgcgcaaa cgtactatat aaaaactttc catttccagg
     9961 aatatgtgca atcaaatcat caacaggacg attgttcgta acattgcacc gaccaccacc
    10021 tgtcatcagt agtttttttc ctgctttttt gtttttttcg atcaagagta cgcgtgcgcc
    10081 ttgttcagct gcactgatcg ctgccatcat cccgcttgtt ccagcgccga ctacgatcac
    10141 atcatacagt ttttccattc cctcacctca aggaaagtgt agcatatctg aagaaagaaa
    10201 acagaaaaaa atgttttgag ccaaaagaga aaatgagcta aaatgaggga agataagcaa
    10261 aggagcgtgg aacgatgaca aacgcaacag aatatgttca agcaattcaa gaaaagctac
    10321 atcaaaagga tcaagggcaa gaagaatttt tacaagcaat cgatgaattc atgcccacgg
    10381 ttatgtcttt tttggacact catcccgaat atatcgaaaa gaatatctta gaacttttaa
    10441 cggaaccaga aagagttatt caatttcgag tgccttggca agatgatgaa ggaaactggc
    10501 gagtaaatag agggtaccgt atccaataca actcagctat cggcccttat aaaggtggat
    10561 tgcgcttcca tcctagtgtc aatctaagta tcttgaaatt tttggctttt gaacagattt
    10621 tcaagaatag tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt gacttcgatc
    10681 ctaaaggaaa atctgataat gagatcatgc gtttttgtca aagtttcatg ctggaattgt
    10741 caaaacatat cggcccatcg attgatgttc ctgcgggaga tatcggtgta ggagctagag
    10801 aaatcggcta tttgttcggc gaatataaac gattgaaaaa atatgatact ggtgtgttga
    10861 caggtaaacc tttggatttt tggggaagta agatacgtac ggaagctact ggttatggat
    10921 tagtgtatta tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat caaaaaacag
    10981 tttttgtttc cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg caagagctag
    11041 gcgggaaagt tgttacatgt tcagattcta gcgggtacat ctatgatcca gaaggaatag
    11101 atgtgaaact attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg gaatacgcaa
    11161 accaacgtcc ttctgctatt tatcatgctg gagaatctgt ttggacattg aaagaaaaag
    11221 cggatatcgc tttgccatgt gctactcaaa atgaaataga cgaaagcttg gccgaaatct
    11281 tagtagaaaa tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt acagtaaaag
    11341 ctgtttctgt tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct gctaatgctg
    11401 gaggagtagc agtatctgct ttagaaatga gtcagaacgt ccagcgattg acttgggaaa
    11461 gagagcaagt ggatgagcag ctggatcaaa tcatggaaac gatttaccat acgtgtcgcc
    11521 aaacagcagc cgagtacggc gacgaaaaaa atctgcttct tggagcaaat gttgctggat
    11581 ttgaaaaagt tgccaaagca atggcagcag aaggtttagt ttgaaaaatg aaactttctt
    11641 ggaaatattg tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa acgtaaagta
    11701 ttacctgtag agtaaaccta taaggaggag aacaaatggc acacattcat tttgactatt
    11761 caaaagtagc accgtttgtc aacgaacaag agttagggta catgcaaagc caagtaacag
    11821 cagcccacaa cgaattacgg gaaggtactg gcgcaggaag cgacttccgc ggctggatcg
    11881 atttaccaac gaactatgac aaagaagaat ttgcacgtat caaagaagca gctaaaaaaa
    11941 tccaatctga ttctgaagta ttagttgtta tcggtatcgg tggttcttat ttaggcgctc
    12001 gtgcagcgat cgacttcttg aatcacacat tcttcaattt attagatagc aacacaagaa
    12061 aagcaccaca aatcttcttt gccggaaatt caatcagctc aacatatatt gctgacttga
    12121 tcgaagtaat cggtgatcgc gacttttcag taaacgttat ttcaaaatct ggaacaacta
    12181 ctgaaccagc gattgcgttc cgtgtattca aagagcttct tgttaagaaa tatggacaag
    12241 aagaagcaaa caaacgtatc tacgctacaa cagataaagc aaaaggtgca gttaaagtag
    12301 aagcagatgc agaaggttgg gaaactttcg tgattcctga tgacatcggc ggacgtttct
    12361 ctgtattaac tgctgttggt ttgttaccaa tcgctgttag tggtgcagat atcgatgcat
    12421 tgatgcaagg tgccgctgat gcaagcaaag catactcaag cgacaaacta gaagaaaacg
    12481 aagcttacca atacgcagca atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt
    12541 tgttgatcaa ctatgaacca ggaatgcaat acttctcaga atggtggaaa caattgtatg
    12601 gtgaatctga aggaaaagac caaaaaggca tctatccttc aagcgcaaac ttctcaactg
    12661 atttgcactc tttaggacaa ttcatccaag aaggacgtcg caatattttt gaaacagttg
    12721 tgaaggttga aaaacctcgt aaatcaatca caattcctga acaagcagaa gatttagacg
    12781 gtctaggcta cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa
    12841 cattgcttgc tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt ccagaaatgg
    12901 atgcttatac attgggttat acaatgtact tctttgaaat tgctgtaggt atttcaggtt
    12961 acttgaatgg aatcaaccca ttcgaccaac caggtgtaga agcttacaag aaaaacatgt
    13021 tcgcacttct aggcaaacca ggctttgaag atcttgctaa agaattgaac gaacgcctgt
    13081 aagataaggg tttttcgcta gatttaaata atcagtgata gaaacatgtt cgctttaaaa
    13141 tgaaaactgg tacccattta ggtactcaaa catactgcat cgtttattca gaataagggg
    13201 ctgtgacaag acgtttgtca cagtcccttg tttgtataga aatcgattga ttgccttgct
    13261 ctttagtttg ttttaggact ttaagaacag tgaggctgac ttcgaaggtt aattctgttt
    13321 ttttgttttc tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt tttgcgatat
    13381 attcttagct taagataggc ctaatttttc ttttacagat gagggtacct tatcgctggt
    13441 aattactttt ttctctatga tccgcttttc gcttgctttc aactgcaaga tcgttccttc
    13501 ggggtaagca gaaccattgg cagagtcacc agaagtaatg acatcgaaat ctaactgtct
    13561 tttttcaccg ttttcagcat aagcatctac gttgtattta aaacctcttc caattacttc
    13621 cccattatct gctttgatat cagtttctgc aggcattgga gctttgacta ccgcataata
    13681 atctttacct acataagtag actgatagta gtcccaacct ttccatccta taaaagcaat
    13741 caatatgata acgatcaacc ctaaaatttt tttcatgaaa gatctccttt tttattttta
//
LOCUS       DASOUD010000069        13759 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.069, whole genome shotgun sequence.
ACCESSION   DASOUD010000069 DASOUD010000000
VERSION     DASOUD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.069"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..2133)
                     /gene="tkt"
                     /locus_tag="U4A29_001888"
     CDS             complement(136..2133)
                     /gene="tkt"
                     /locus_tag="U4A29_001888"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO0681008.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2235..2486)
                     /locus_tag="U4A29_001889"
     CDS             complement(2235..2486)
                     /locus_tag="U4A29_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO0681009.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2633..3256
                     /gene="lexA"
                     /locus_tag="U4A29_001890"
     CDS             2633..3256
                     /gene="lexA"
                     /locus_tag="U4A29_001890"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEO0681010.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3435..3653
                     /locus_tag="U4A29_001891"
     CDS             3435..3653
                     /locus_tag="U4A29_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681011.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3941..4453)
                     /locus_tag="U4A29_001892"
     CDS             complement(3941..4453)
                     /locus_tag="U4A29_001892"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO0681012.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4467..6764)
                     /gene="recJ"
                     /locus_tag="U4A29_001893"
     CDS             complement(4467..6764)
                     /gene="recJ"
                     /locus_tag="U4A29_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO0681013.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6906..7349)
                     /locus_tag="U4A29_001894"
     CDS             complement(6906..7349)
                     /locus_tag="U4A29_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEO0681014.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7350..8138)
                     /locus_tag="U4A29_001895"
     CDS             complement(7350..8138)
                     /locus_tag="U4A29_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO0681015.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKACDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8135..9076)
                     /locus_tag="U4A29_001896"
     CDS             complement(8135..9076)
                     /locus_tag="U4A29_001896"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO0681016.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9253..9840)
                     /locus_tag="U4A29_001897"
     CDS             complement(9253..9840)
                     /locus_tag="U4A29_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEO0681017.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10061..11368)
                     /gene="obgE"
                     /locus_tag="U4A29_001898"
     CDS             complement(10061..11368)
                     /gene="obgE"
                     /locus_tag="U4A29_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO0681018.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAQENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11661..12671
                     /gene="gap"
                     /locus_tag="U4A29_001899"
     CDS             11661..12671
                     /gene="gap"
                     /locus_tag="U4A29_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO0681019.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATENSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12756..13691)
                     /locus_tag="U4A29_001900"
     CDS             complement(12756..13691)
                     /locus_tag="U4A29_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO0681020.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3790 a         3095 c         2425 g         4449 t
ORIGIN      
        1 aattctttgt tttgtcattt aaatgtaaca tagccgaaaa cgaaaagtaa tattgattta
       61 ttcttctaac acataaagaa cagctgtctc caacttgcag acagctgttc ttatcagtca
      121 aacaattgct tggatctatg atagttgatt gtacgtattc acgacatttt ctactgtaaa
      181 tccaaattct tctaaaattt tagtgccagg ggcagatgca ccgaaatgat cgattgtaat
      241 cgttgcgccc tctgttccta cgtagcgttc ccatccaaat gatgcagcag cttcgatagc
      301 cacacgtttc ttcactgatt taggaagtac actttcttta tattcagaag attgtttttc
      361 aaagaggtca aagcttggca tagaaacaac agaaacatct ttgccttttt cagccaattc
      421 tttttgagct ttcacagcca aatcaacttc agaacctgtt gcaatcaaaa taccttctgg
      481 tgtttctcct tgtgatggag aaagaacata cgcgcctttt ttcaccattt catctgctac
      541 ttcttttgta gaaggcaata ctggaaggtt ttgacgactc aatacaagga tagtaggcgc
      601 atctgttgtc tccatcgcta ctttccaagc agcacgtgtt tcatttccgt ctgccggacg
      661 aatcacttga acaccaggca tgcagcgaac agatgctagt tgttcaatcg gttcgtgcgt
      721 tggtccgtct tctccgacag caaccgagtc atgtgttaag acgtaaacaa caggagtatg
      781 ttggattgct gctaaacgaa ctgctggtct tagataatcg acaaagacaa agaatgttcc
      841 accgtagata cgagttccgc catgaagttg gataccattc atagccgaag ccattgcaaa
      901 ttcgcggaca ccgaaccaga tgttgcgtcc ttcataatgt tcaggagtga aatctttatc
      961 tgcagccacc attgtattat tggaacctga caagtccgct gaaccacccc agaagcttgg
     1021 tatagctttc gataattctt ggatgacttc tttacttgaa acacggcttg cttgactgct
     1081 tccaacttca taagttggca attctgcatc ccagttttct ggtagtttgc catcaaaagc
     1141 atcttcaaat tgttgtgcta attcaggata agctttttta taattggcaa acatttcatt
     1201 ccatgcatct tcagcttttt gtccttcttc tatcatcgtt tgatggaagc gggcagctac
     1261 ttcttctgga acagtgaaat ctggatactc ccaaccataa acttctttag ccatcttgat
     1321 gccatcttca ccaagaggag caccatgaac agcagatgtt ccttctttag gagcaccata
     1381 gccgatgact gtttttactt cgatcaatgt tggtttgtct gtttcagctt ttgcttcttc
     1441 aatcgcttta gagattgctt ctaggtcatt tccgtctttc actaagatat gttgccagcc
     1501 atatgcttca tagcgagcac caacgttttc tgtgaaagct tttgaagttg gaccgtctag
     1561 tgaaatatcg tttgaatcgt ataaaacgat caatttccct agtttcatat gacctgccat
     1621 cgaacttgct tcttgagata ccccttccat caagtctccg tcaccgcata ttgcatacgt
     1681 atagtgatcc atcacattaa agttttcttt gttatacgtt gctgcaaggt gcgcttctgc
     1741 cattgccata ccgacagcca ttgcaatccc ttgtcctaat ggccctgtag tggcttctac
     1801 accatctgta tgatgtactt caggatgtcc tggtgtttta gaatcccatt gacggaattg
     1861 tttcaaatca tcgattgtca cttgatagcc agctaaatga agcaagctat acaacattgc
     1921 tgacccatgt cctgccgaaa gaacgaaacg gtcgcggtca gcccagtttt ttgatgtcgt
     1981 tggatttact ttcaggtgtt ttgtccataa cgcatatgcc ataggtgctg cgcccatcgg
     2041 caatccagga tgtcctgagt ttgctttttg gactgcttca atacttaatg tacgaattgt
     2101 gttaacgcca agttggtcaa ttttgtcaaa caaaataacc cctcctatta atttaggttt
     2161 tttttacctt tatttcttac actttcattc tataagatgt cacacgaaaa agcaatcttt
     2221 ttcagtattt tttactaaaa gttgtcattt cggttgtgta agcctttttt cttttggatt
     2281 tcttttaatt tttccggggt cacgtcattt ccctcaggat cgacgacttt cattccttcg
     2341 atatgatgac gcataccgga acgaaaagct tccaaatact ctttgcgcaa gttgatttgt
     2401 tcttgttctt cggcttttga gagaccattt tccttctttt tacgtgcaag ttcattgatc
     2461 cgctctattt tttcaggtga cagcatgctc agccctcctt ttactctcct tcatattata
     2521 taaaaactga aaaaaaaaca actagataaa ctaaataaaa tacgaacatt cgtttgattt
     2581 agcgcttttt atatggtact atttatttat caagtgaaag aaggtgtacc ttgtggcccg
     2641 acaaactgaa tcaagacaaa tcgaagtctt acgatatatc catgaacaag taacccaaaa
     2701 aggatacccg ccgactgtcc gtgaaatcgg ggaagctgtt cagctttctt ctacttctac
     2761 tgttcacggt catcttgcac ggcttgaaaa aaaagggttt atccaaagag atccgacgaa
     2821 acctcgtgca atcgagttga caaaacaagg tttagaaaag attgggattc gtccaacaac
     2881 tattccagta cttggcgtcg taacagctgg agaacccatt ttagcagtag aggatgcttc
     2941 agacttcttt ccaatccctc ctaatctttc ttccgaagaa ggcagtctct ttatgctgac
     3001 aattcgtggg gatagtatga tcaatgctgg gatcttggat ggtgacaacg tgatcgttcg
     3061 gaagcaagaa actgctcaaa atggcgatat tgtcattgca atgactgccg aggacgaagc
     3121 gacttgcaaa cgattctata aagaaaaaaa ctttttccgt ctgcagcctg aaaacgactt
     3181 gctcgaacca atcatcttag atcaagtatc tatattagga cgagtagtag gactttatcg
     3241 tagtcatatc tattaattgc tactaataaa agaaaacttt ttaaaaagag tttgagacga
     3301 tggtctcaaa ctcttttttc gcccaggttc ttcttctttc aaatttttat cacttccctc
     3361 tttacaaacc gaacaaatat tcgtatacta aatatacgaa caattgttcg cataaattaa
     3421 cgaggtgaga attcatgaaa tcaatgagac gtcttggctt agtattaact gttttgtttt
     3481 taggtattat tattggtaat ttcggcatac gtccagcttt acttgtgttt gttccgttat
     3541 ttactttgtg gttcatgttg tgggatgaaa agaagtaccg tcaatctgag cggcgcagaa
     3601 ttcatgaaga tcatttctat catgagccct actacagatc caggcaatca taataaggat
     3661 tgactgctaa ttattcaaaa gacaagaagt taaactttta cgggggtgtg acaaaactcg
     3721 gtcacatcct tttttcgaat aaacggtgtt ctaaagccag tttttcggta ttaagtcaaa
     3781 ttgcggaaaa agtgattttt taaacttcta ctggtctttg cttgtttcca tttataaaaa
     3841 aatagcgacc ctcaaaagtc agatagagaa tctgactttt gagggtcgct ttaaaatcgc
     3901 ttctttaaga ataacatcaa gaatagcttc ttttttctgc ttaatattcc attaatgtaa
     3961 tgatgtcata tcctttgatt ttgtcgcgtc catggagatc catcaattcg atcaaaaatg
     4021 cgcagcctac gactacaccg cctaattgtt cgatcagttc gatcgtcgct ttgattgttc
     4081 ctccagtagc tagcaaatcg tcacaaatca gcacacgttg acctggttta atggcatcct
     4141 tatgcaatgt caatgtatct gttccatatt ctaatccata agtcacttcg atggtttctc
     4201 gaggtaactt cccttttttg cggactgggg caaaaccgac tcctaactca taagcaacag
     4261 gacatcctac gatgaatcca cgggcttctg gcccaactac catatcaatt cgtttttctt
     4321 ttgcataatt tacgatttgc ttcgtagctt cccgataagc gtcaccatct gccatcagtg
     4381 gggaaatatc tcgaaacaca attccttttg acggatagtc gggaatactg gcaatataat
     4441 cttttaaatc cactctttgt ttcctcctag gttgttaacc atttctttat ggtaggaata
     4501 tcgctcaaaa gcaaaaattc ctcaactttg attttattga gccgccgctg gtataatctg
     4561 ctctcagtca aaggatgact ttctgggcta gcgacttttt gtaaaacacc gttctctatt
     4621 gtaacaaatt tcaattcaaa aaacacctgt atcataaaaa ttaataattt ttcagggata
     4681 cgcaaatatt gagctattgc ttttatttta tgacgtatat caattcgttc ttgagaatgg
     4741 atcagtttaa acaattttgc gtactgttct cttgtgccta ccccattcaa ataggcctca
     4801 tcaggcgaaa tccccatcag ataaacacga ttgaatgaat aagaccgaaa aacttctttc
     4861 agatccgcta aatcatctgg acaatccata ataactagag agttcggttt tcttttttca
     4921 attattgcct ttacagaatc tagtccttcg aaaacaatcg cagcttcctg gataatctca
     4981 ggcacgaatt tcaacgtaga agaatcaaat gctaaataaa ggctaccttc cgacgttcgg
     5041 ctctgttcac ggaaacgttt tgcccgccaa tcaaatactt gcagaccacc cacagaaaaa
     5101 tcagatacca tcagctgcgg tttcttacgc ccgttccatt cattgatcga aagctttcct
     5161 accacatcta cctgattatt cagcagttct tcttcttgag taccaaaacc aaaagcgacc
     5221 gcatcaagct gactcgatgc atcggaaagg acaaatttca gatgctgttg gtttgctccg
     5281 attcttttga tattttctgc ttggacttgt cgaaataaaa actgaggcaa aggattatcc
     5341 gtaccaaatg gcgccaatag tttcagctca tcaattcgct ctactgttac ttcatttggc
     5401 agcaatactt catcaattct caactcaggc cctctcatca agtcaatttg atgatcgact
     5461 atatattggt tcatcttctc ctgtagtata gtgacatttt cacttggcat cgttaaaccg
     5521 actgctgcat gatgtcctcc gaagtaagta aataaatctc gcatctggtc aagcatttca
     5581 aaaagattca aggcttcaat actacgtcca gatcctttag ctgatccatc ttcttttaaa
     5641 gtaaggacaa gcgttggttt acccgtttca ttcattattt tcccagcaac aatgcccaaa
     5701 acgccttcat gccatcctcg gtttaccaat agatggatat gattctcctt attgaccatc
     5761 gctaatgctt cttgtgttat ctgctctacg attgcttttc tttcttcatt gatctcattc
     5821 aatttttttg cttgaacgga tgcttcctct tcatcaaacg tcgcaagtaa cgagacggca
     5881 ggacgtggat cgcccatccg accgattgca tttagtctgg gagcaatcga aaaaccgatc
     5941 gttgtttcgt ctgcgtccct catttttacg ccgctttctt caaatagcgc ctgaagtcct
     6001 atccgttctg aatgatgaat ggcatcgatt cctaatgaaa caagaattcg attctcatca
     6061 gtcaatgaaa ctaaatcggc gatcgttcct atcgctacta aatccaaaaa ttctacaggc
     6121 ggttcttcca ataatgcaga agctactttg aatgctactc cgactcctgc caaatctgga
     6181 aatggatagt gccccgctgg atgacgaggg tgaacaatcg cataagcgtc tggtaaattc
     6241 tcaggtagtt cgtgatgatc tgtaatgatc acgtctacac cagcttgctg agcgtatgct
     6301 accgcatcat ttcctgctac cccgttatca acagtgatga tcaactggat tccttcttcg
     6361 atcttttctt ggtatacaga ttggtttggt ccatacccat gctcaaatcg atttggcagg
     6421 aatacttcta cttctgcacc aagtaattcc agtgtttctt tcattaccgt tgcactggtg
     6481 atcccatctg cgtcataatc accgtaaacg aggatttttt ctccactgat tacagcttct
     6541 tggattcggc tgattgcttt atccatatcg tgcatcagat acggatcatg taattcttgt
     6601 tcctttggat gtaaaaatcg gttgatatcc ttttcctcat ggtaacctct ttgccacagt
     6661 aacttaccga caaatggaga taatttcagt tcacggatca tttggagaaa ggattcagaa
     6721 ggctctgtct cgcttaactt ccaatcaaaa tttgcttgtc tcactttttc actcctaact
     6781 tagttattat agcaaaggaa atcagcaaaa aaaagcagaa agaaagaata aaaacagatc
     6841 agatccgcta gacagatgtt cagcaggacc tgatcttttg tacgaccatt tatgaaatat
     6901 ccctattagt caaaataatt gaagctttgt ttgggcgatt gagttttttg ttcttccggc
     6961 gctttcaatt gactgatttg tgcggttttc gcttgtaact ccgcctgata agcagcttcc
     7021 aactccgcac gttcgtttct aaactcgcgt tgctgttctt cttttgcttc ttcaattttc
     7081 ttttgaagtt ctgtttgata ttctgacatt tcctgagtca gctgtttgat ttgctttttt
     7141 tgctgccata gtgttgccga agcagtcaaa aatacaatca gtgcaccaat cagcgcagaa
     7201 ccgatgatca ctaagatcaa tggcgcagaa aaggaagtga agccaaaact tacaggaaca
     7261 tttttattgt tcaatacagc aaaaagaaca ataatcaaaa ctaaaacaaa tccaagaatc
     7321 acattgcttt gctttttcaa ttccttcacc tactttttgt taaaaattcc gctggctaaa
     7381 taatcaccaa tcgttggaaa cagcgtataa aattttgatg caacttctaa aataaacggt
     7441 tgattgattt cccgttttgg atgtctcata ttgcggacga tcatttttgc caacttggca
     7501 ggttctaaaa cgatttgtcc gactttttcc aaatagcttc cgcttggatc agccttatcg
     7561 aagaaatctg tctcgattgg acctggattc actgtcgtaa cagctacgcc aagtggtttc
     7621 aattctaatc gcaaagcgtt ggagaagcca aggactgcaa atttcgtggc agaataaacc
     7681 gttgattttg cagttgccat ttttccagcc atagacgcga tattaatgat atgaccttgt
     7741 cttctttctg ccatatcgat cgcaaatttt tgtgtcaaaa ccatcattcc taagatgttg
     7801 acttcaaaca tatcgcatgc ttttccaagg tcgaatgtaa ggaagtcttc gaaaatccca
     7861 aaacccgcat tgtttaccag aatatcgacg cgcccgactt cttccatcac cttttcatat
     7921 aattcgtcca cgctttcagg attgctgacg tctaccttga aagcataagc ctctttgccg
     7981 ctcaagcgtc tgcactcgtc tctgacacct tctacaaatg ccattcttcg tgcgcaactt
     8041 ataacgattg ctccttgctt tgctgcttca taacagattt gtgcgcccag accgcctgaa
     8101 ctacctgtta ctaaaacaac tttatcttct aaattcatgc ctcttcctcc tcgcgaaatg
     8161 gaatctcaat gctatcaaaa tctttcatga tcgtcgtgtt ttcaaacact tcttgtgctt
     8221 ctttttcaag ctctaaagct gcttttccta aatatctcgc actaatatga gtaagtagca
     8281 gccgttttgc ctgcgcttct tttgcaactt cagcagcttg atgagtcgtg gaatggaaat
     8341 agttgtgcgc cattcttgct tcatctttat taaatgtgct ttcatgtacc agaatatcgc
     8401 tattttccgc aagcacaacg gaattatgtg tttttctcgt atcgcctaaa atagtcacga
     8461 ttcgtccttt tttatctgga cctacgaaat cttttccatt gatcgtccgc ccatcttcta
     8521 gttggatcgt ttcaccttgc ttcagtttgc cgtacagtgg tccagctgga atacctaatt
     8581 cttttagacg atccacctgc aattctcctt tatgatcatg ttcaaccaca cgataaccaa
     8641 aactgtcgat cccatgattt agtttttttg tataaacaga aaattgctga tcagtaaaaa
     8701 tcggggctgt ttcatttagc tcaatgaatt tcagcggata acttaaacgc gtttgcgaaa
     8761 tacctaaaga gacttttatg tattcctcta tcccttttgg cccgtaaatc tctaatggcg
     8821 tatccccacc ttgaaatgac cggctgctga tgagcccggg aagtccaaaa atatgatcgc
     8881 cgtgtaaatg tgtaatgaaa attttaccga tttttctcgg acgaatagtc gttcgcaaaa
     8941 tctgcatttg cgtgccttcg ccacagtcga acaaccaaac ttcattgcgc tcgtctaaaa
     9001 gtttcaatgc tatacttgtc acatttctat gttttgcagg aacacctgca cctgttccta
     9061 aaaattgtaa ttccataatc tacctgactt tctttaagtt cctctttaat tttagggtaa
     9121 aaagaaggga taatcaaccg taagtcgaga atgggttcaa attaaaaata acgttctaaa
     9181 gcgatagcgt tttctttgaa aattactata aaaacagcag cgaacccgtt tgagatcctc
     9241 tgctgttttt tattaatttg aagatccaga actttcattg tcgttggaag tttgcggatg
     9301 tttgtccttc caagtctttt gtgcagcttc tttcgcatga ttttcagctt ctttcgcatc
     9361 atgcacatct ttttcgacat cttcataatc caagcgggta atttctccgc ctaattccat
     9421 cattaccaat tgattcagtg ggcgattgtc ctcttcatca gcaatcaaga tcagtcctgt
     9481 agctccttca ccgattttat ttcctacaaa atcgaacacg gtctgggcat cttttatttc
     9541 tttggcatct tgtgtcgcac cgatcattcc tccggcaaac catcctaata aaatccctag
     9601 cggaccacct aatacaccaa ccaacattcc gattaatcca cccgtagaag atttgttatt
     9661 accggtaaag tcgataaaat cttctatttt gaattgatgt gtcccattag gatcatggct
     9721 gacaactgcc atctgttctc ctttaatcga acgaacagca tgtaactttt tgatttctga
     9781 aaatgcctga tatgcctggc tttctacttg aaaatttaaa atgattactc gtttgctcat
     9841 taaaaacgcc cctttcatac cttcttgtta tttattttac ctttaattgt aaaataagaa
     9901 aagaaaagca ctcttttttt ccaataaaat agggctgtga caaaagtcgt atcacagctc
     9961 ctttttccga ataaacggcg ttcttgaagc tgaccacttc tgtcacaacc tcccttaaat
    10021 ctgaatctat attttttggt atttgttttt atttgcttgc ctattcgaca aattcaaatt
    10081 caaactcacc gatacgtaca aggtcgccat ctttcgcacc gcgtgcccga agagcttcat
    10141 ccacacccat accacgtaat tgacgagcaa aacgcatcac tgtttcatca tgatcgaaat
    10201 ttgtcatttg gaataatttt tcgatcttct caccagataa gatccacgtt gcatctgaat
    10261 cacgatcaat ttggaactca ggaccttctg gattgaatcc atagtgtaca gtttcttctt
    10321 ccagttcttc ttcgtacaat gggaattctg gcgttacttc taacaaatct gctgtagcat
    10381 tcaataacgc gtccaatcct tgtcgagtca ctccagaaat tggaaatact gggatgtcat
    10441 cagcaaattc atcttctttt tccttgttca gctgttcttt gaatttcact agattttcct
    10501 gtgcatccgg catatccatt ttgtttgcca cgatgatttg cggacgctct agcaagcgta
    10561 agttataagt agataattct ttgttgatgg caagatagtc ttcataagga tctcgtcctt
    10621 ccatcccact catatcgatc acatgtaaaa ttactcgtgt tctttcgata tgacgaagga
    10681 attgtgtacc cagcccaaca ccttgcgaag ctccttcaat caagcctggc aagtctgctg
    10741 cggcaaaact tctgccatca cttgtagtaa ccattcctag attagggacg agcgtcgtga
    10801 aatgataagc gccgattttt ggacgagcag atgaaataac ggatagtaaa gtcgatttcc
    10861 cgacagatgg aaaacctaca agtccaacgt ctgccaatac tttcaattct agttcgattt
    10921 ttcgctcttg tcctggttca ccgttttcag caatttccgg tgcaggattc cgaggagagg
    10981 caaaacggat attccctcgt ccaccgcgtc caccttttgc cacaactagt gtttgaccat
    11041 tttcaattaa atctcctaaa agagcaccag tctccgcatc tcggacagtg gttccttgtg
    11101 gtactttaac ataggtatct tctgaaccac gaccgtgcat ccctttactc atcccatttt
    11161 cacctggctg cgctttgaaa tggcgattga accgaaaatc catcaatgta cgtaaccctt
    11221 catcgacgat caagatgaca tcgccgccac gaccaccgtc tccgccagcc ggtccgccgt
    11281 caggaacgta tttttcgcga cgaaatgcaa ccattccgtc tccgcccttg ccagctttca
    11341 catcgatcgt tacttgatct aaaaacatgg acataattaa gtcctcctat tattcattta
    11401 tttatttgac tgcctgaatt tccacaataa acaaaagtct tacttatttt atcggtgaaa
    11461 tctgattttg tctatcattt cagcaaaaag attgttacct tcttcttttt ttatcatttg
    11521 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11581 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11641 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11701 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11761 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    11821 cttatcctgg cacagttact gcaacagaaa actcaatcgt agtggatggc gaagcaactc
    11881 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    11941 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12001 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12061 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12121 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12181 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12241 gcggcaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12301 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12361 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12421 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12481 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12541 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12601 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12661 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12721 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12781 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    12841 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    12901 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    12961 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13021 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13081 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13141 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13201 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13261 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13321 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13381 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13441 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13501 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13561 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13621 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13681 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13741 atcaaagaat ctctactat
//
LOCUS       DASOUD010000070        13706 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.070, whole genome shotgun sequence.
ACCESSION   DASOUD010000070 DASOUD010000000
VERSION     DASOUD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.070"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..902)
                     /locus_tag="U4A29_001901"
     CDS             complement(<1..902)
                     /locus_tag="U4A29_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO0681021.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="U4A29_001902"
     CDS             1185..2153
                     /locus_tag="U4A29_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO0681022.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="U4A29_001903"
     CDS             2266..2544
                     /locus_tag="U4A29_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO0681023.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="U4A29_001904"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="U4A29_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO0681024.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="U4A29_001905"
     CDS             3001..3756
                     /locus_tag="U4A29_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO0681025.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="U4A29_001906"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="U4A29_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO0681026.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="U4A29_001907"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="U4A29_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO0681027.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="U4A29_001908"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="U4A29_001908"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO0681028.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="U4A29_001909"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="U4A29_001909"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEO0681029.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="U4A29_001910"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="U4A29_001910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO0681030.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="U4A29_001911"
     CDS             8493..9035
                     /locus_tag="U4A29_001911"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO0681031.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="U4A29_001912"
     CDS             9058..10614
                     /locus_tag="U4A29_001912"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO0681032.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="U4A29_001913"
     CDS             10628..11530
                     /locus_tag="U4A29_001913"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO0681033.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="U4A29_001914"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="U4A29_001914"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO0681034.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="U4A29_001915"
     CDS             12973..13395
                     /locus_tag="U4A29_001915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO0681035.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2452 c         2988 g         3736 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag actttt
//
LOCUS       DASOUD010000071        13597 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.071, whole genome shotgun sequence.
ACCESSION   DASOUD010000071 DASOUD010000000
VERSION     DASOUD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.071"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..266
                     /gene="yaaA"
                     /locus_tag="U4A29_001916"
     CDS             24..266
                     /gene="yaaA"
                     /locus_tag="U4A29_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO0681036.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            253..1377
                     /gene="recF"
                     /locus_tag="U4A29_001917"
     CDS             253..1377
                     /gene="recF"
                     /locus_tag="U4A29_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO0681037.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1374..3320
                     /gene="gyrB"
                     /locus_tag="U4A29_001918"
     CDS             1374..3320
                     /gene="gyrB"
                     /locus_tag="U4A29_001918"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO0681038.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3340..5811
                     /gene="gyrA"
                     /locus_tag="U4A29_001919"
     CDS             3340..5811
                     /gene="gyrA"
                     /locus_tag="U4A29_001919"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO0681039.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6047..6346
                     /locus_tag="U4A29_001920"
     CDS             6047..6346
                     /locus_tag="U4A29_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO0681040.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6396..6908
                     /gene="ssb"
                     /locus_tag="U4A29_001921"
     CDS             6396..6908
                     /gene="ssb"
                     /locus_tag="U4A29_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO0681041.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6934..7170
                     /locus_tag="U4A29_001922"
     CDS             6934..7170
                     /locus_tag="U4A29_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO0681042.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7313..9286
                     /locus_tag="U4A29_001923"
     CDS             7313..9286
                     /locus_tag="U4A29_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO0681043.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGESAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9292..9744
                     /gene="rplI"
                     /locus_tag="U4A29_001924"
     CDS             9292..9744
                     /gene="rplI"
                     /locus_tag="U4A29_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO0681044.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10018..11385
                     /gene="dnaB"
                     /locus_tag="U4A29_001925"
     CDS             10018..11385
                     /gene="dnaB"
                     /locus_tag="U4A29_001925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO0681045.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12165..13457
                     /locus_tag="U4A29_001926"
     CDS             12165..13457
                     /locus_tag="U4A29_001926"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO0681046.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4527 a         2465 c         2995 g         3610 t
ORIGIN      
        1 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
       61 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
      121 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
      181 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
      241 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
      301 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
      361 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
      421 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
      481 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
      541 aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
      601 gcaccagaag atctttcttt agtaaaaggc agcccgcaga ccaggcggaa gtttttagat
      661 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
      721 ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
      781 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
      841 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
      901 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
      961 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
     1021 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
     1081 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
     1141 ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat
     1201 gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga
     1261 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
     1321 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
     1381 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
     1441 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
     1501 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
     1561 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
     1621 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
     1681 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
     1741 ttcacggagt aggatcttct gttgttaacg cgctttctac acagcttgat gtcaaagtat
     1801 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
     1861 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
     1921 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
     1981 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
     2041 tcaatgaata ccattatgac ggcgggatta aaagttatgt cgaatacttg aatgcaaaca
     2101 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
     2161 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
     2221 tccatacgta tgaaggcgga acacatgaat caggattcaa gactgcttta actcgtgtga
     2281 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
     2341 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
     2401 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
     2461 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt caaattgttg
     2521 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
     2581 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
     2641 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
     2701 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
     2761 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
     2821 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
     2881 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
     2941 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
     3001 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
     3061 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
     3121 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
     3181 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
     3241 tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg
     3301 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
     3361 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
     3421 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
     3481 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
     3541 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacta tgcgatctat
     3601 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
     3661 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
     3721 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
     3781 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
     3841 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
     3901 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
     3961 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatgggaaaa
     4021 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
     4081 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
     4141 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
     4201 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
     4261 cgtcgtgacg ccagcgcatc tgttatcttg aataacttat ataaattgac atccttgcaa
     4321 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
     4381 aaacaaattc ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag acggacagtc
     4441 tttgaaaaag aaaaagcaga agcaagggca cacattttag aaggtttgcg tattgctttg
     4501 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa
     4561 gcgacgatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
     4621 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
     4681 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
     4741 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
     4801 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
     4861 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
     4921 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
     4981 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
     5041 cgggcaaaag gatacgaaat tccagaatac gggcgcacag ccaaaggaat accaattatc
     5101 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
     5161 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
     5221 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
     5281 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
     5341 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
     5401 ggggttagag gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
     5461 gaagatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
     5521 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
     5581 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
     5641 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
     5701 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
     5761 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata gccatcaaga
     5821 ctaattgtgt aagctagcaa gaatgaccga ttaaaaaatc tggttattcc tgctggcttt
     5881 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
     5941 caaaatagag tgtataatca gagcttgtga gtagtctaat taattagata actctccttg
     6001 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
     6061 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
     6121 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
     6181 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
     6241 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
     6301 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
     6361 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
     6421 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
     6481 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
     6541 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
     6601 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
     6661 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
     6721 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
     6781 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
     6841 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
     6901 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
     6961 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
     7021 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
     7081 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
     7141 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
     7201 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
     7261 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
     7321 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
     7381 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
     7441 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
     7501 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
     7561 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
     7621 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
     7681 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
     7741 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
     7801 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
     7861 tgccattgat aaattggatg acaaagagat ttcttatttg aactctttcg tcactacact
     7921 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
     7981 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
     8041 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
     8101 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
     8161 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
     8221 tgtttattat gggggggaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
     8281 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
     8341 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
     8401 caaccagaaa aaagcttaca tcgttatcaa cgagaacgag atcattccag atgtagaacg
     8461 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
     8521 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
     8581 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
     8641 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatatcgaat cttcagcttc
     8701 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
     8761 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
     8821 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
     8881 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
     8941 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
     9001 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
     9061 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
     9121 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
     9181 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
     9241 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
     9301 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
     9361 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
     9421 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
     9481 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
     9541 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
     9601 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
     9661 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
     9721 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
     9781 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
     9841 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
     9901 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
     9961 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    10021 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    10081 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    10141 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    10201 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    10261 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    10321 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    10381 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    10441 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    10501 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    10561 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    10621 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    10681 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    10741 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    10801 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    10861 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    10921 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    10981 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    11041 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    11101 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    11161 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    11221 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    11281 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    11341 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    11401 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    11461 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    11521 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    11581 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    11641 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    11701 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    11761 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    11821 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    11881 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa
    11941 atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc
    12001 acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag
    12061 cgtttttata aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt
    12121 gatacaatgg ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag
    12181 tagtaggtac gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa
    12241 atgctgaagt gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta
    12301 acggtgtgac ttataaactg catttgattc cttctgggat tttttataaa gacaagatca
    12361 gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt
    12421 tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct
    12481 tgccttatca tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg
    12541 gtacaacgat taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc
    12601 gtattgcaga cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg
    12661 acaaaaaccg tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt
    12721 ttgaagaata ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta
    12781 tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa
    12841 tgctggatat cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg
    12901 gggtaacaat cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca
    12961 aagcctatac gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag
    13021 gagaacaaat ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg
    13081 taggctggtt tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc
    13141 tatctttaaa ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt
    13201 atgaattaga tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct
    13261 gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac
    13321 ttgcagaact gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg
    13381 tacgtatctc aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg
    13441 tctgggcgca aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga
    13501 gtcgaagtgt ttatctatac gataataata acttgaacac tgtttattca gaataaggga
    13561 ctgtgacaaa aaccgtgtca cagtcccttt ttccgaa
//
LOCUS       DASOUD010000072        13337 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.072, whole genome shotgun sequence.
ACCESSION   DASOUD010000072 DASOUD010000000
VERSION     DASOUD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.072"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            43..363
                     /locus_tag="U4A29_001927"
     CDS             43..363
                     /locus_tag="U4A29_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO0681047.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(568..792)
                     /locus_tag="U4A29_001928"
     CDS             complement(568..792)
                     /locus_tag="U4A29_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEO0681048.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(990..2570)
                     /locus_tag="U4A29_001929"
     CDS             complement(990..2570)
                     /locus_tag="U4A29_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO0681049.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2971..4347)
                     /locus_tag="U4A29_001930"
     CDS             complement(2971..4347)
                     /locus_tag="U4A29_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO0681050.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4475..5593)
                     /locus_tag="U4A29_001931"
     CDS             complement(4475..5593)
                     /locus_tag="U4A29_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO0681051.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5735..6169
                     /locus_tag="U4A29_001932"
     CDS             5735..6169
                     /locus_tag="U4A29_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0681052.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(6247..6780)
                     /locus_tag="U4A29_001933"
     CDS             complement(6247..6780)
                     /locus_tag="U4A29_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO0681053.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6874..8052)
                     /gene="mutY"
                     /locus_tag="U4A29_001934"
     CDS             complement(6874..8052)
                     /gene="mutY"
                     /locus_tag="U4A29_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO0681054.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(8045..8842)
                     /gene="recX"
                     /locus_tag="U4A29_001935"
     CDS             complement(8045..8842)
                     /gene="recX"
                     /locus_tag="U4A29_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEO0681055.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8949..10331
                     /gene="rlmD"
                     /locus_tag="U4A29_001936"
     CDS             8949..10331
                     /gene="rlmD"
                     /locus_tag="U4A29_001936"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO0681056.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10505..11083)
                     /locus_tag="U4A29_001937"
     CDS             complement(10505..11083)
                     /locus_tag="U4A29_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEO0681057.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11405..11875
                     /locus_tag="U4A29_001938"
     CDS             11405..11875
                     /locus_tag="U4A29_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEO0681058.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11983..13209)
                     /locus_tag="U4A29_001939"
     CDS             complement(11983..13209)
                     /locus_tag="U4A29_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO0681059.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3941 a         2688 c         2247 g         4461 t
ORIGIN      
        1 agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt ggtaaatgta
       61 acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc ccatctagtc
      121 aaggtctaca cagccggaaa taccactatt gtctacactg aagcccctga acataatgaa
      181 attctgctga tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga tgctaaaaat
      241 tatttcttag ataaaatcaa agacgccgtt taccacgagg atgaaatcaa agtaatcgaa
      301 ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat taggcgctac attagccgtg
      361 taaacaaatt ttgaacataa taattaaaga gcgtgggaca aaagtaaaga acacttttgt
      421 ctcacgctct aaacacgtat aaacggcgaa agcagaagca actccttcgg aaataagctg
      481 aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct tggagttaaa
      541 cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt tcttttgtac
      601 ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt cctctttatg
      661 gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt ttacatctaa
      721 taatcgacat aatcgtaaca tctcttcttt tttctgtttt tttctttcac gtctttgcca
      781 ttctgtttcc atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa ataattttta
      841 tatttttttg attagacatt tctatgattt taatatagta aaaagagccg tcacggaaaa
      901 tccgtcacgg ctctaaacaa aatatgagag gctgtttttg tttggctatt cttattactt
      961 gatgcagggc agcttgtgtc aagacttctt tacatcaaac tttttaattt tacattggga
     1021 tatttatccg caaaccagcg ttctgcaaat tggttctcga ataagaacaa cggttggtcg
     1081 aaacggtcac gagccaagat attgcgactt gaactcatct tctcatccaa atcttctgga
     1141 tcgatccagc gagcgatttt gtgtcccatt ggagacatga ttacttcggc gttatactca
     1201 tttaacatac gatattggaa tacttcgaat tgtagctgac caacagctcc aatgatatat
     1261 tcttccgtta gatatgtttt ataaagctga atcgctcctt cttgaactaa ttgatagatt
     1321 cctttgtgga aggatttttg tttcatgaca ttttttgcag tcactttcat aaacagctct
     1381 ggagtaaatg aagggagttc ttcataagca acttttagtt ttccttcgaa caatgtatcc
     1441 ccaatctgat aattgcctgt atcatataca ccgatgatat cgcctgctac tgctttttcg
     1501 acgttttctc tcgaatctgc cataaattgt gtgacattgc ttaatttcag ttttttattt
     1561 gtacgttcca accagacatc cattcctcgt tcgaatgttc cagaacagat tcggacaaaa
     1621 gcgattcggt cacgatgggc tggattcata tttgcttgga tcttgaatac gaaacctgaa
     1681 aattcttcct cataaggact taccatctga tcatcttgtg ttttgtgtcc ataaggagct
     1741 ggtgccaatt caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt caatgcagaa
     1801 ccaaaaaata ctggtgtctg atcgccacga gcaatttttt cttgattaaa tgcatctcca
     1861 gcctctttga ttagttctac ttcttctaag acttgttcat ataaggaatt ttcagttaga
     1921 ggatgatttt cgggaagcag tccatcttca tccaattcta ctaatcgttc atcctgataa
     1981 ttttccggac gataaaactc tacacgatta tggtagatat cataaagacc ttctagccct
     2041 ttacccatac cgattggcca attcatcgga taagattcga tatctaatag ctcttctaat
     2101 tcttctaaca attccaatgg ttcacgacca tcacgatcta gtttgttgat aaaagtaaat
     2161 atcgggatgc cacgtttttt tacaacttgg aataatttct ttgtctgtgc ttcgatccct
     2221 tttgcactat cgattaccat gaccgcacta tccacggcca tcaaagtacg ataagtatct
     2281 tcagaaaaat cttcatgtcc tggtgtatcc aagatattta ctcgtttccc ttgataatca
     2341 aactgcataa cagaactagt aacagaaatc ccacgttgtt tctcgatttc catccagtct
     2401 gatttggcaa aattacctgt ttttttgcct tttacggtcc cggcttgacg gatagcccca
     2461 ccaaacaata gtaattgttc tgtaatcgtt gttttcccag catccggatg ggaaatgatc
     2521 gcaaaagttc tgcgattgtc cacttgttgc tttaattttg gattattcat aacctaactc
     2581 catttcttcc attcaattat ttatattttc tctaggcaca gattgtccga tctcttaaag
     2641 aaagacaatt tcatataggg ctgtgacaaa agccgtgtag cagtctcttt ttccgagtaa
     2701 acggcgttct agagacagcc tatcagcaat aagcccaccc aagccaaaaa tacgaaaagc
     2761 tgtttttgct tggctgctct tattacttaa tgcaggatag ctttttcaca acctttgtca
     2821 actactacaa ctgaatcagt atagcatgga ttcagcgaat gaaaaagcca ccaaaccata
     2881 agagtcaaat atttttttgt cagctgacta aaagaaaaga cgtgttgctt gtttattcaa
     2941 gcaacacgtc tttcataacc aagtttccaa ttatcttctt ggctcgtcat cttcgaattc
     3001 ttttctgaag tatcttcgtt cttcttcagg ttccgcttct gtttcttccg gatcatgaaa
     3061 aatgacacgg attttaagga ttcgagaacc ttccatttct tcagaaacca gcgtaatatt
     3121 atcgacatca aacgataact tttcgccttc atccggaatc atgcctaatg ctgtaatcaa
     3181 ataaccagcc atcgtatcta catcactcat atgcagatca ctgtcaaaag cttcattgaa
     3241 ctcatcaatc agcatacgtc cttggatgat atattcgtgt tcatcgattt tttcataaag
     3301 attctcaact tcatctgatt catcatcgat ttctccgacg atttcttcta aaaggtcttc
     3361 tagtgttgct aacccaacga cgccgccata ttcatctaat aaaatagcca tctgattctg
     3421 cgtctttttc atttcatata acagatcatc aataaagacc gtttcaggaa cgaataaagg
     3481 ctcctgcatg attttcttga tatctaaatt atcaaagccg aatttatggg cagcttttag
     3541 taaattcttt gtatgaagga ttccgactac tttatctttg tcttcgttat aaacagggat
     3601 tcttgaatag ttttcggata aaacttcatt cacattttct tccacagtgt cgttgatatc
     3661 aatcataaat gcatccgttc gtgggaccat gacttctcgg gcaactttcg tatcaagaga
     3721 aaaaacccct tggagcattt ctaattcttc attattcaat acgccttcgt tttcaagcat
     3781 ataacgcatt tcatctctgg tcatttttga atcttcatca tcaaaagtca tcggcgtgat
     3841 acgcgccaat aaacttgtgg aagcagaaag cagccagacg aagggcttag ctactacacc
     3901 aatcaagcgt atcagccctg aagtgaactt tgcgacttct tctgatttat ttaaagcaat
     3961 acgtttaggg tacaattctc caaaaacaat cgatacatag gtcaaaatag ccagcacaat
     4021 gatatttgca atattttttg ccgctgtgcc gccacctaat accggagcta accgttccga
     4081 taaagtatta gctagtgaag caccagacaa aatgttgacc aaagtgattc ctacttggat
     4141 tgtcgatagg aaattatttg gattctgcaa tatttttaaa agcttttgag atttcacatc
     4201 tccttcatca gctttttgct cgattcgatt cttattgatc gaaacaacag caatttcgga
     4261 agctgctaaa aaagcattga tcaatgtcaa aacgatcaat aataaaatct gcgctaacag
     4321 cgactgactc tcagggtcag cattcataat cgcattctct ccttataaaa ttaattttta
     4381 gtttacgata gaaaaaaacg tggcactata gccacgttaa aatggtatca caaagcaatt
     4441 ttctttgcaa taaaaacaag tgtactaaaa ctacctacga ggtcgtagca attttcttct
     4501 tgttttcatt ttgcttgtct tctttgacga ttttgattat ataggaaatg atcgtcttgc
     4561 agattgcgta aaaaggaaca cctaagaaga taccaagcaa tccggcaata tttccagcta
     4621 ctagaagaac gataatgatc gttaatggat ggattttcag tgatttccca attacattag
     4681 gataaatgat attcccatcc aactgttgga cgattagcac aaccacacaa caaagtaatg
     4741 cttttaccgg atcattgaag actgttacta gaaatgcagg tgctagtcct agataaggtc
     4801 ctaaatatgg aattaggttt gtcaatcccg caatgacccc aaataagaaa gcataatcca
     4861 caccaattac catatatcct aaaaatgtga aggtaccaac gaataaacat tcgattgctt
     4921 gtccgctaat gtaattagcc aatgttttat tcagttgccc caataactcc acaatttggt
     4981 ttcttctttt ttctgggaaa aactgttgga tgtttggcac tagtttttct ccgtctttca
     5041 acatatagaa caagatgaat ggtgctgtaa tgattactac ggttgccgaa gcgatcgttc
     5101 caacgatcga acctaaacta ttggatactc cactaaggaa ttgctgaatg attgtcccgt
     5161 aagaaatgtc taattgctca aaatatttat tgatatctac ttccttgaac aaaggcaatt
     5221 cggctacctc ttttaaccag ctttctacct gtttgatgaa atcaggcata ttagatgcca
     5281 atgaggaaat ttgctgtact agacttggga tgacactaag taaaatccaa ataattgctg
     5341 caactaaaag gagcagcaca attgcgacag cccatattct tttgattttt gctttcatca
     5401 acagcttgac aataggattc aataaatagt aaagaaagcc tgcaatcagt acaggtgcaa
     5461 ataacgtgga aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct gatgaaatca
     5521 tgatcaatgc agctatgatc aataattcaa gcgaccaaaa cattagttta gactgtctaa
     5581 gtttttcaaa caatagattt cctcctttct ttcttactag tatatattat attcgttaaa
     5641 aattctttta ttcttttttt atttttgaaa acttgccata ctttttattt gttttatttt
     5701 ccagtatact atacgaaagg aaggtggcta gaccatgaaa gttgtacaaa caaaagatac
     5761 aatgagcaat atttacttag atgctgtacg gatcaggcat caagtttttg tagtcgaaca
     5821 gggtgtgcct ttgtctcgag aaatagacaa agatgaagcc cattgtatcc acttcgtcct
     5881 gtactcagat aaaaaagaac cgcaaggaac tgtacgtctt ttgcctttag aaaacggaaa
     5941 aatgaaatta cagcggatgg cgattctttc tgaatacaga caccaagggt taggaaaaat
     6001 attgattgaa gaagcagaaa actttgctaa gaatcaaggc tataatacga ttttattagg
     6061 ggcacagtcc acagctgaaa ctttttacga aaaactagga tatacagctt acggcgatcc
     6121 ttttgaagat gctggcatgc ctcatattta tatgaaaaag actttataaa aatttatttc
     6181 gaaaaaagca aaaaacagca agcttttgag aaaaatctct cactggctta ctgttctttt
     6241 ttgtgtttac ttacgcgaca attgcttgta acggttatac caaatatcaa tatagccttc
     6301 cgagaacggg ccatctccat tattgatcca gtcaaccaga atcttcacat tttcttttag
     6361 gatgaagtca atatcgttcg ggtaattcat cattttgctg tgcatctcat attcatcgac
     6421 atccaatagt cgtttctcgc cgtcaggaaa taccttgata tccaagtcat agtcgatata
     6481 tttcaaggcc tcatcgtcca gcaaaaaagg tgaagctaga ttacaataat aggaaacccc
     6541 cttttctctg atcattgcta ttacattgaa ccagtatttt ttatgaaaat aaacaatcgc
     6601 tggttcgcgc gttacccatc ggcgtccatc agattcagtg actaatgtgt gatcattcac
     6661 gccgatcata gagtgttcgc ttgtttttaa taccatggta tctcgccacg ttcgatgcaa
     6721 tttgccgtcg tgcttgtaac tttggatcgt cacaaactct ccttcttttg gaactcccat
     6781 agcaaccctt ccttaagcag cattttgtgc tttgtgtttg ggtgttcatt acaccgacat
     6841 cataattatt atatcaataa aaatgagctt tgcctagaaa ggattcctat tgtttcgatt
     6901 tttctctaac tgatctacca gtttcatttg gaccttagga aaaacgaccg aatcaaatgc
     6961 atcaggcttc aaccacttcg aagttttgtt ctcggtaaat tcttgttctg ctccctcggt
     7021 tgcccgtccg taaaaaagga gaacatgcca tttcagatga ctaaaaatat gagtaacttc
     7081 tcctaaatgt cgtgtctgcc aaacaaagag ttcctgcttt tcaaatggga gattttgctc
     7141 ttcttctgct actaggtcat caaacagatc gatttctggc tttgtttccc attctttttt
     7201 caatcgctca tactcctctt gtgtcacttc catcattgga aatgtccaca tattcgccag
     7261 caatttttgg ctatctcttt cttcaaaata atacgctcca gaatgatttt gaagagctgc
     7321 actgatataa taaacgtctt ttggctttgc tttttttgtt tttacaggaa aactcgtttg
     7381 gattcctcgt ttgttagcta aacaaaaggc ttggatcgga caagtctcac actttggaga
     7441 agttggtgta cagatagctg aacctagatc catcattgct tggttgaatt cacctggatg
     7501 cttttcatca atgattttcc gcatcgcttc atcaaagatt ttcctgctag aagcttttgc
     7561 gatgtctgct tcaatgcaaa acaatctgct gactactcgc atcacgttgc cgtcaactgc
     7621 aggttctgga agtccaaatg cgatacttgc aatcgcgcct gtcgtatatg gtcctattcc
     7681 cttcaatgag ctaatttctt caggcgtttg cggcatttcc ccatcaaatt ctgacatgat
     7741 ctgtttagca gcagcttgga tgttccgggc tcttgagtaa taaccaaggc cctcccatgc
     7801 ttttaaaagc ttttcttctg gagcatttgc tagctcttca attgtaggaa accattccat
     7861 gaaacgataa aaataatcga tcactgtgtc tactctggtt tgctgaagca tgatctcaga
     7921 gatccagata cgataaggat cacgattata gcgccaaggc aggtttcttt tttcttgttc
     7981 ataccattga atgaactgat cttgaaattc ttttgtttct ttatctgtcc atttttccca
     8041 ttctttattc actttcctct tccttttccg taatgaatcg ctggatcata tccaaatcaa
     8101 atcctttttg atataagctt tgctttactt ttagatttct ttttttaggt tcaaacctta
     8161 aatttcttcg ccatagttta tctccttgtt tgactaaata atcatattct atttcttgat
     8221 ctgcttcttg cggaagatca gctatcgctt gttggatcac ttcttgagaa aagcctttcg
     8281 tcatcaatgt ttgttggatt tttctcaata attctttttg gctttttccg tgattttttc
     8341 caaacgcttt ttcggctgtc tgatgtgcat tttgaacttg gtcttcaaat gtatattggc
     8401 tgagcgcttg ttcaatgatt tcaggtttca aacctttttg ctgcatctgc tgcgctaatt
     8461 tctttggtcc tttgtcactt aagcgcatat tcgtgcgcac ataactttcc ccatagacca
     8521 aatcatcgac aaggtccaat tcttttaagc gggctacgat tttatttcga tcttcttgaa
     8581 ggatttcctt ttcttttaag tatgttcgga tttctttttc cgatcgcaac tgataactta
     8641 agtaattcat tgctaggtga aaaccgaaat cagctccgct actcttcttg atctcttgga
     8701 tagtttcttc gtctaattcc cgtcctttta ataatcgata gcgtactaat acatcttcag
     8761 atacttgtaa agcttctcct tcagataaat ctacgcgata gaaagggcct ttgcctttac
     8821 tgattctcgt aacggttaac ataaacatcc tcctcgaaca tatattctct atcataccac
     8881 agaaaatccc cgtaatcagg tattatatgt tagaataaac cacagaaaaa agaaaaaggg
     8941 agatacttat ggcagaaaca attgtcaaaa ctggacaaac ggttcctttg aaaatcaagc
     9001 gtttaggcat aaatggtgaa ggaatcggtt atttcaaacg gttgatcata tttgttccat
     9061 atgcactacc aaaagaagaa gtactagtga aaatcacgaa agcaactcct cgctatgcag
     9121 aaggagaatt aataaaagtc aaaaaaacaa gcaaagatcg cgtagtggca ccatgtcctg
     9181 tttactatga atgcggtggc tgtcaattac aacacttagc ttatcaagct caattggatt
     9241 ttaaaaagga tctgcttttg caagcattgg aaaaattcaa accagcaggc tttcgatctt
     9301 atcaactttt gccgacgatc gggatggatg agccttggca ttatagaaac aaagcacaat
     9361 ttcagttacg aaaaaataag cagactcaaa aaattgaagc tggtttatac gaagcaaact
     9421 ctcatcagtt agtccctata acagactgct tggttcaaga acctcgaaca caaaaagtaa
     9481 tgaatactgt tgttcaacta ttgaataaat ttgatgcacc catctatgat gaacggacaa
     9541 acagtggaat cttccgcaca atcatggtta gaatcggtgt taaaactggg gagttacaag
     9601 ttgtttttat tactcgcagt aaaaaattcc cgcaaaaaaa cgcaatgatc cgtgagatca
     9661 atgaacgact gcccgaagtt gtttcaatca tgcaaaatgt ccaggcacaa aaaacgtctc
     9721 tcgtgatggg ggatgaaacg atccacttat ggggaaaaga agccattgaa gaacaaatca
     9781 atgaagtgac tttcgatctt tctcctcgtg ccttctttca actcaatcct cagcagaccg
     9841 aattgcttta tggcgaagga atcaaagcat tagatgtcca gcctaatgaa acgatcgtgg
     9901 atgcttattg cggagttggg acgatcggct taagtgtagc aaaacaagcc aaagaagtac
     9961 ggggaatgga taccgttcct caagcaatcg ctgatgcaaa aaaaaatgcg gaaagattag
    10021 gatatcacaa cactcgatat gaagtcggga cagcagagca agttctgcct aaatggttga
    10081 atgaaggttt tcagcctgat ggtatcatcg tagatccacc acgtacgggt ttggataacg
    10141 cattgatcca atcattattg aaataccctc caaaaaaact tgtttatatc tcctgtaatg
    10201 tttctacact tgccagagat ctggttaatt taagtaaaaa gttcaagatt gaatatttac
    10261 agtccgtcga catgtttcct cagactgcac gttgtgaagt tgtcgtgaag atgacgagac
    10321 tcgctgacta aatgaaagag gttgtgaaaa agtctgccct gtatcaagta ataagaacag
    10381 ccaaacaaaa atagctcttc atgttttatg aatactgtac ttgtgaccga agagttaaca
    10441 ggaggacact atttatttaa aaaaagggtt gtgacatagt ttttgtcaca gccctttttg
    10501 attattattg gatattcacg tctatcactt gataaaatgc atttgtcgta tcgctgatat
    10561 tccatactgc taaaatcaca ttatatcctt ttcgatcctg cggaagtgtg atcgtttggt
    10621 tgactgtttt tggtggaaca gttgctttgt cgtctatttt tgtgatcaat tcaaaatcac
    10681 taaatttcaa aggttgatta ggattccaag ttggtttggt aatgtagtaa tcccaagtac
    10741 ttgtccgatg ttgtgctgtt aaattccaag taatatttaa tgttcctgga ttaaccatgt
    10801 ttttatgcca tctgttagat gtctgttcat ctaaaggctc aaagccgctg actcctgcac
    10861 tagcaatttt tccatcaatg aatgtatttt tggtagcttc gatactttgc ggttcccatt
    10921 gtgctcttcc gacattttga tttaagtttc ctgctgctga tgttcctagg tatgcacggc
    10981 ttcctggaga agtaatataa ccatgcgcaa aacctggaat gctaaaaccg aacaacccta
    11041 aactaactaa cccaataaac aataacgtac taatcttttt catgtagaaa cctccttcat
    11101 ttgataacca cattgtaaac gctttattgt attaacacaa acattatgct tttataattt
    11161 tatgttgaag taaaaaaggt gaaaaaagta agatgcagat tataacagaa gtagacgaga
    11221 aaagtacaaa tagaaaacat gagaatcaaa tcatatgatc tttttcaaaa acatcattca
    11281 tataaacaaa tgttcaactt tttaataaca acaattatca atttttttct tacttcacaa
    11341 actaaataca ggtcttgctg tgagccttca tggtaaacta atattataga aaggagttag
    11401 taaaatgaag atgataaaag atattcgttt ttgggtttgt gttataggaa ttgtcatatt
    11461 aggcttttta tcagggttgc ttagcggaaa tcccggaaaa tattactatt ctttgcaact
    11521 tccaccattt gcaccaccta gctggatatt tgggccgatg tggaccttac tgtatatatt
    11581 gatgggtatt tctctttatt tgttacttaa tcacaacaac aaaaaacagc gaaacaatct
    11641 tgtcggtctt tttgtgattc agttcatttt taattttata tggtctgctc tattttttaa
    11701 tttaagaaat attttcattg cagcaatcga tattacactt ttagttattt tcttgtcagt
    11761 gcttatgtat cagttgtggc tgcatcatcg cctggcaatg tggctcatga ttccttatta
    11821 tttatgggta ctattcgcaa ctctattgaa ttattcaatt tattttttaa actaagcgct
    11881 aaaaagagac gtcctgtaat ttaacacata gaaaaagtgc cacctaagtt agattttttg
    11941 tctaatttag gtggcactct ttattaacca aactattttt ttctagtacc cacttgttgt
    12001 tccatactca ttattctggt cataaccata accattttgt tcctgtccag cgtaaggatc
    12061 ataactttga gtatcttgac ttggtgtata gtactgactt gtatcttctg tttgctgata
    12121 ataggtattc ggatcttggt actgatcata tgtgttttct gaataggaag aataatcggt
    12181 gtacccttca ctattcgatc catcaccgtt tgtcgaatcg ccatcttccg aatcatcaaa
    12241 gaacatcatt gaagcattct catttttcaa attaggtaat gtatcgcttg tttttatatt
    12301 taattgtttc ttcagttcat tttggatcga aagaagatca ttttttccta aaatttgata
    12361 gtagatatca ttgatcattt gtccttcgcc ttgcaactgg tcttgtttga ttgtttcaaa
    12421 cgccggggtg tagttcgtcg ctacatcaag catgtcatcc caatctaagt ccgtcgtcac
    12481 attgttctca actgctttta aaatttttct ataattgctt acgccatcta aacttaaaac
    12541 tttattgacg attttcgtca ctacttcccg ctgacgacgt tgtcgaccga tatcgccttc
    12601 tggatcttcg tgtcgcattc gagaataggc taaacctttt tctccattca atgtttgttc
    12661 acctactggt acatggacac catccaaagt aaagtcaatt ttattattca ctttgatgcc
    12721 accgaccgca ttgatcaaat cttccatgcc atccaaattg atcgttacat aatgatcaat
    12781 aggaatatcg agcagttgct caattgaatc catggccatt tcgacaccac caaatgcgta
    12841 ggcatgattt aatttatcta ccgttccata acctacgata ttcgtataaa tatctcggtc
    12901 caaactgatg atcgttgatt ttttttgttt cggattaact gttactacca tcatcgtatc
    12961 agaacgtcct tgttccgttc ttcctaatcc accggtatct aaacctaata gcaaaaccga
    13021 aaaaggttct ttgtcatcaa tgcttactct ctcagaacgt ttggaggata ttcgattaga
    13081 gtttttagaa attttgttta ctgtttggct cgcttctccc ataaatctga ttccgtatgc
    13141 tgataatccg gcaatcagca aaacgaatac ccctaacaag gttaatagaa ctttttgcca
    13201 tcgcttcata atacacctct gtttcacatt ttctaaaata ttgtctcata taactgttca
    13261 atcagcaaat tcacttttta atcataagaa aactttaaaa aacatttttg tgtaacgatt
    13321 attatttttt aaaattt
//
LOCUS       DASOUD010000073        13010 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.073, whole genome shotgun sequence.
ACCESSION   DASOUD010000073 DASOUD010000000
VERSION     DASOUD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.073"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(214..897)
                     /locus_tag="U4A29_001940"
     CDS             complement(214..897)
                     /locus_tag="U4A29_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEO0681060.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(915..1532)
                     /locus_tag="U4A29_001941"
     CDS             complement(915..1532)
                     /locus_tag="U4A29_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO0681061.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(1542..2366)
                     /locus_tag="U4A29_001942"
     CDS             complement(1542..2366)
                     /locus_tag="U4A29_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEO0681062.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(2359..2919)
                     /locus_tag="U4A29_001943"
     CDS             complement(2359..2919)
                     /locus_tag="U4A29_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681063.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            3093..4049
                     /locus_tag="U4A29_001944"
     CDS             3093..4049
                     /locus_tag="U4A29_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO0681064.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(4354..5310)
                     /locus_tag="U4A29_001945"
     CDS             complement(4354..5310)
                     /locus_tag="U4A29_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO0681065.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            5414..6016
                     /locus_tag="U4A29_001946"
     CDS             5414..6016
                     /locus_tag="U4A29_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEO0681066.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(6104..7603)
                     /locus_tag="U4A29_001947"
     CDS             complement(6104..7603)
                     /locus_tag="U4A29_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEO0681067.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7619..8143)
                     /locus_tag="U4A29_001948"
     CDS             complement(7619..8143)
                     /locus_tag="U4A29_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEO0681068.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            8441..9190
                     /locus_tag="U4A29_001949"
     CDS             8441..9190
                     /locus_tag="U4A29_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO0681069.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9181..9966
                     /locus_tag="U4A29_001950"
     CDS             9181..9966
                     /locus_tag="U4A29_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEO0681070.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            9935..10651
                     /locus_tag="U4A29_001951"
     CDS             9935..10651
                     /locus_tag="U4A29_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO0681071.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(10753..11787)
                     /locus_tag="U4A29_001952"
     CDS             complement(10753..11787)
                     /locus_tag="U4A29_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEO0681072.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(11791..12849)
                     /locus_tag="U4A29_001953"
     CDS             complement(11791..12849)
                     /locus_tag="U4A29_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEO0681073.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         3807 a         2683 c         2337 g         4183 t
ORIGIN      
        1 gtttttcatt ctatagaaag cgcttcaata aatcaagaca tttttttgtt agaatgctaa
       61 taataacaaa aggttttaac tcttcttatg tacattacag gtaaaaaaac aaaaaaattt
      121 ctcttttttt tgaattctga acaaatagta ctggagtaca aaagacgagg aagagccact
      181 atctgctctt cctcgtcttt tgattctcac ctgttattca tgattctttt tctcagtttc
      241 ccgatatttt tccattttac gactaaaaag ctctccgcta cttggtggcg tatatgtgat
      301 ggcatttgct cctgcttgaa tggtctcagc aatactttct tccgttggac cacctgtcgc
      361 aatgatagga agttcaggat attttttacg aaaatggcgt actgcttctg cagtttcttt
      421 acctgcactg atattgataa tcgaaatacc agctgctaac ttttcatcta tttctgtata
      481 tttcgacgtt accgtactga taactggaat atcaactact cgacaaactt cttttactgt
      541 ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat
      601 gctcatgtag gaagaacgag caccttgtgt caaaccacca cctacaccgg agaaaactgg
      661 aatgtcagct gcctcaataa tgcttttagt aatcgctgga tgaggtgtaa aaggatacac
      721 agcaattact gcattcgcat ttgtattacg tatgatcgca atatccgtag taaagatgat
      781 cgaacggatc aattttccaa atatcttgat cccgcttgct tctaaaatga tttccggtac
      841 tcttacgata tcttttctca attccgtcat gatttctggc gtaaattgtt ctgccatcag
      901 ccacttctcc tttgctatct ccggtcatac tgattcaatt gatacgtctc gatgacctgg
      961 atgatttttt gcgcataacc ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg
     1021 gcttcctgat agttggtggc cgtaatcact ttttcataca gcgcaggatt ccaatctact
     1081 ccttggacga acagttgtgt atggtcatcc atggactgtt cccatgaatc atacactttg
     1141 aagtctcctt ggatagttat ccattcttcg ttaacaaatt cctttgtttc caggcttact
     1201 ttcttctgat ccccataagc cttgatcccg aaaagattat tgtatttgct agccaaggta
     1261 ctttttcccc aatttgattc taatatagct tgtcctaaaa tgatactagg aagcactcca
     1321 taaccatctt gtagttcttg ggcatgcgga acaagccgtt caataaattg ctgatgggat
     1381 tgcgcttcat tttcttgctg attatcagta aaaggagaag acagattctt caaggaaaaa
     1441 acaaaggcta agagtatcac tgctacaccg gcaaccactg ctggccaacg gatattcttg
     1501 cgcttttgct ttttccactt ttttttcgcc atgatcaatt cctacttttc atttaattca
     1561 ttcagatatt cttctagcgt caattggtgt ttggtgatat actcagcgtt ttctttaccg
     1621 acataacgta agtgccaagg ttcatatgtg atcttagtta tatcttcgtg tccttttgga
     1681 taacgaacga taaatccata ctgcgcagca ttatctgcga tccatttagc tccaggctga
     1741 tctccgtaac ttgcatccaa tacagtagaa ggataactgt tgtaccagtt ttgatcgacg
     1801 acatcgactg ccagacctgt gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta
     1861 gtaatctttg tcgcctcctc ttctgataca ccttgacgac tcatcacatc ttggacattt
     1921 tgttcgaaaa cttgttgttg ggatgataca gaccggtaag ctgagaccat cactaatgga
     1981 aatccagctg cttcagcagc cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg
     2041 atcaagtatc cgttacttaa tgatgccaat tgagtagact catcaatttc tttttctaat
     2101 ttgtgatctg gaccaaccaa aactagcttc caatcgtctg ttttaacatc tggcaattgt
     2161 ttggacgtac gatcttttgt ttctttgtcc tttaccttgc tgctggttgt ttgactgttc
     2221 ttttctttac tagcagttgt tttggtgctt gttcgttcga ctgcttgatt gccggcttga
     2281 acatgcgaat catcatcgct aaaaataaca tagcccattg ctgcgatcat aatcacaact
     2341 gctccaaatc ctaaaaattt attcacttga aaaatccttt cgttgttctt cacgttcccc
     2401 attcgcctca tgaacgtaag tttcttccag attttcattc acccaatcga gaagttcgat
     2461 tttttctcct tcgatctgtt tttgaacatc tgctggcaga cgaaaattca aaatatcatc
     2521 atagccccat tcatcatatt gaatacttac tttcaagtca aggtatctac cagcctgctg
     2581 attatttttc aaagggatca gctcgacttt tgctgctttg ctgctaagcc atttttgcgc
     2641 gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg tttcgttttt cgggaaaaat
     2701 aatcgtttga cgcaaaatct tttgcgttag catccattca taatcagtga aaagattttt
     2761 tactttctgc atcgcttcag atggcagatt tttctcgcca tctttccatt tttgccattg
     2821 ttctggactg atcagcaagt attcatctaa aaaatttttt tcagtttcaa actgttctaa
     2881 taatacactc ataataagat caatatatac ttgatgcacg tttatcccct ccttttaact
     2941 attctactta agttttgatc aaaaagcacc tcttaaatcg ctgctttcat tttttttaat
     3001 gtttcaagtc ctctcctctt tattcttcgt gatttttgtt ataataatag acagacaaga
     3061 aactcaatcc aactatgaag gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg
     3121 gtcttggtac catcgcacat gagtttgccg aaagcttcaa tcaagagacg agcgaactaa
     3181 cagcagttgc ttccagaact tcagaaaaag cggaaaactt cgctcaccgt tacaatattc
     3241 caaaagctta cggaagctat caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca
     3301 ttgctgtacc aaatagacag catatcgacc atatcttggc agcattggaa gcaggcaaac
     3361 acgttctatg tgaaaaagcc atcacgatga ataaaaaaga actggctgac gctatgcgct
     3421 tagcagaaga aaagaatttg atcttagcag aagcgatgac aatcttcaac atgccacttt
     3481 atcagcagtt acgctcaata atggatacag gaaaactggg ggctttaaaa atgatccaag
     3541 cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg gttcttcaat ccagaattag
     3601 ctggaggagc tctgttggat atcggtacgt atgctgtatc tttcgctcgt ttctttttga
     3661 gctctcaacc tgaagtagtt gcctctacaa tggttccttt tgaaacaggc gtggacgaac
     3721 aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc
     3781 aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa cggttatgtg acgattgctg
     3841 attacccgcg tgctgatcgt gcggagatct tatttacaga tggtacaaag gaattcatcg
     3901 aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa gaatatggta cacatgattg
     3961 aaggtgactt gcccaatcgc tctctcttct tgacaaaaga tgtcatcgaa atcttggatc
     4021 aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca
     4081 tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt ggctggactt
     4141 attaccgcag aggaaagctt ttgaacaccg tttaagaaag aggttgtgag cctaccgttc
     4201 agctccgagt cacaaggagc aatttcaaaa aacagcttct catgtttgtt gaatattgtg
     4261 acttgtgacc gaggagttga caggagaaca ccgtttaaag aagaggttgt gaaatcagct
     4321 ttttgacctg aatattagct gaattcacaa cctctatttt tggatttttt gttgggagaa
     4381 ttctgccttg atttcaataa aacttttaaa agactgcaaa aactggaaca attctgctcg
     4441 atcctcgaat tcagggcgac tagtcggcag aggcatttgc cgatacaata cataaacttc
     4501 ttcaatacgt gttaaaattt gtctgccatc atttgactct gaaaatgttt ctgcagtata
     4561 aatcaataaa ccatagatat gttttccata ataagctggt tgctggattc tctctagatt
     4621 ttccagcatg tctttaatca cattggcctg tgctcttctc atagagaagt aagtttcgta
     4681 gtatagcggc tgtctgagcc agtagttttc ttgatgttct ctggcatctt cttggctttc
     4741 gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca tcttctttat tttctgataa
     4801 aagaaattct gccatttgtc tcaaaatatt tcgaaattct ttttcgatct gtatttggtt
     4861 ttccttcagc tgcttcgtac gatctggcat gtaaacattt gcaagcaaag caaatactaa
     4921 tccaatcgcc attaatcccg cttcattgcc gatcagctgc caagacatgt tttcctccac
     4981 taaataatga gtgaccaaaa ccgaatttac tactatccct tcggttaatt tgaagcgaga
     5041 ggcaagcggg atgaacagga ataaaaaaag actgaagcca aaaatagtat acccaacagc
     5101 aaaaaaactg aacagtgctg ttgccgtagc taatataaag gctgcgattc gattcatgcc
     5161 cgtacgcaat gtagagcgct tggtatttcc tacacttaat aatgcaatga tccctgcagc
     5221 tggccaatac aacagcgata agctacccgc tgtgaccatc gaaaagaaga cactgatcac
     5281 tgttttgact gttcgcattc ctatacgcat ttacttcccc cttttttatt ttaattatac
     5341 ttgaataatg aaaaactagc tatcagacaa aagtttaggt atgatagtaa aaagaccaaa
     5401 aaggagaatc tctatgcaat ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc
     5461 cttagctgtt tttttatgta tcttgttttt tcatttgact caacgaggaa tcccgatgat
     5521 tgctgcatta tctgctgttt tttctttgcg tcaggacttg actacaaccg tttcttttgg
     5581 aaagtctagg atcatcggca atacattagg tggtacatta gctatcattt atttttttgt
     5641 caaaagctat tttcacaatg attttttggt agaattgatc ttgcttccta ttctagttat
     5701 tattgtgatc gtcgtgtcag atggtattaa caataattcc ggtatcattt ccgcaatagc
     5761 tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa tccgctttgt atgctatcca
     5821 acgtgtacta gatactttta tagggacatt cattgcaatc ggtatcaatt tctttcttcg
     5881 tcctcctgaa acagaaaaga aggaagaact atcagaagat ttggtcgaac tccaaaagaa
     5941 agaacgattg ctaagaacaa atttacaaga gatagaagct aagatccaaa aacaaaagaa
     6001 aaatagcgag gactaatttt cttttgcaat aaaataaaaa aatggagaga agaactttaa
     6061 aaagtcttcc tccatttttg attgaacggt gaagtgtact actttattct tccttcatcg
     6121 agttttgttc tgcctctttt ggcgcttcat acatatttac ttggctagta gtcccacgtg
     6181 tcgacaaatt ctctacgatt tccttcaagt caagtcctgt tgtcgctttt agtgtttctt
     6241 gagtagaaga aagcaaattt gtggcataat ttgtcactcg gttcgctcca ctggtttctc
     6301 ctgaactgct tcctgtatcg acaacagaga ttttttcgat atttcctagc ggctgtgctg
     6361 cttcgcgcat caattgcggt agcatatcga taaccatact caagacagct gcttcgccat
     6421 attccttgaa ggcattggcg atcttttgtt ttgcttcggc ttctgctttt ccttttgcta
     6481 gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt attggcttcc gcttctgcca
     6541 gtgcttccac acggaatcgt tctgcttcgg cttcagtcac ttctttgact ttttgagctt
     6601 gtgcttcttg ttctttagcg taccgatcgg catctgcttt tttcttcact tccgaatcat
     6661 attgcttttc acgacgtgtg atttcttttt cttccagttc gatttgtttt tggcgttcta
     6721 cgattttaat ctgcatttcc tgttcgatta cttgttgttg tgctcgggca ctttctaaat
     6781 tgtaggcttg gtctgctttt gctttagcaa catcttgttc ttgtttgtag gttgctaatt
     6841 tcagttcttt ttcttttaat gattctgcga tctctgtttg tcgctgtaat tcagctgctt
     6901 gtgattcttt ttctgcttga gcttttttga tacgcgtttc ttttagagct tccgcttctg
     6961 ctatatctgc atctcttttg acttgggcga ttcttggttt ccctaaagag tcaagataac
     7021 cgtttttgtc tctgacctct ttgatagtga aagaaacgat gatcaacccc atttttgcaa
     7081 ggtctacaga agcgacttct tgtacgcttt gactgaactt atcgcgattt tgatagattt
     7141 cttctacagt catagaacca agaatcgagc gcaagtgtcc ttctaatact tctcttgctt
     7201 cattttccag ttcttctctt gtcttgccta aaaactgttc tgctgctgta gcaatctctt
     7261 ctacagatga accgattttg atgatcgaag taccatcaca catcaccggt acgccttgtt
     7321 ccgtatacac ttcaggggtg gagacgtcca atttactcga taatagactg atccgattcg
     7381 aacgttgaaa aactggcaag acgaatgcgc cacctccacg cacaattttg attttgttat
     7441 tgctttcatc aatgtgcaca tttttgcttc ctaaataact tccactgatg atcaacgctt
     7501 catccggttt tgctgtctgg tacttcgcta caaagacgat cagcaacatc agtaaaatca
     7561 aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc catttaacac acgctccttt
     7621 aagcttttat catgctttca taaggcatga cataacagat tctttccttg atctcaatga
     7681 tcaatacttt cgttccccgc tcagctgtct gttcttgggg gctgtaaaaa caagctggac
     7741 gactaatgga accggtaaca cttttcaact gaatttctcc catacctcgt acagggatcg
     7801 gtgtgatcac aacagctact tgtccttcca gagttttttc agaagacgac atcgttgatt
     7861 cggcatgttt catcggcatc aatatataga aattaagcaa aaataccaac actatcgata
     7921 tgcctccact agcaataaat aaaaataaag atgattgatc tgttaaacgc tcacctagaa
     7981 aaccaaataa gcttgtgaaa gttaaccaag ggataataag catcggatca agcgggccat
     8041 caaaatcgaa tacatctcca aaaataatca ataggactgc caatcctgca caaatcagca
     8101 atgtatacca ataaatcgtc tcaagtgcga ttccccctat catatacacg cctccttcca
     8161 tttctacgtc tatcatacca atgtttgaaa attctgtata gcttctttta ccgactcagt
     8221 ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt tgttaaattt ttgttttcat
     8281 gttaaaattt tcacaataaa ttctttcgag gtagcctatg tttccttttt ttaagaaaaa
     8341 accattaaag ctccagaaca cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt
     8401 gattgtttct gccatattga tcaggaattt tattctttcg atgaaccgat ctagtgtgtc
     8461 agataaaatc tctagcattg ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct
     8521 gcagactggt gacccgacac atgcgattca aggaataaca aatgaaatca tgattgcatc
     8581 gaatttagat tttatcgttg tcatggaccc tgaaggcatt cgatattctc acgcagaccc
     8641 aagttacatc ggtcttcctt tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca
     8701 agaatttttc tctgaaaaag aaggaatgtt agggttcggc ttccgctatt tcgtccctat
     8761 ttattatgat caagagatta ttggtgcagt ttgtgttggc ttaacaaaca acacgattga
     8821 caatcaagtc aaagatggac aaaaaatgat tgttttttca ttgattattg gacttagcgt
     8881 aggcatcata ggcgccgttt ttcttgccca aaaaatgaaa aaagtactgc ttggacttga
     8941 accacaagaa atagcagaac agttaagtga aaaaacgatt atcgaaaatg aagtagatga
     9001 aggaatctta gccatctctt ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa
     9061 cattttctac caaacagagc agctattgga tttcgaagta ggaaaaaaat cagtgataat
     9121 ctatatgacg ttttgtttaa agagacgatt gagcatcaaa cgaccgttcg gactcaatcc
     9181 atgtatttaa atgggataga aactatttct agcgttacac ctctttttag caatgaccgt
     9241 tttttaggag cagtcgcaac tttacgaaat cgatcagaag taatgaaatt aatggaggag
     9301 cttgggggga cgaaacaata tataaactcc atgagagctc aaacccatga attcatgaat
     9361 aaacttcatg tcatttctgg tttgattgat ttgaagaagt atgaagaagt cacccatttt
     9421 atccagcagc taaatcaaaa ttataaaaaa gagattggcc aagtctcaag tctgatcaaa
     9481 gtacctgcaa ttgccggttt tttattaggc aaaataaacg aagccaaaga acaatctgtt
     9541 gaaatgctgt tagaaagcga ttcttttatc cctaaaatgg agatggatga ctccatccat
     9601 tcacttttgc aagtgttagg gaacttactt gataatgcaa aagaagccgt gctacaaaaa
     9661 catgaaagaa acggacaggt ccggattcat ttaaactatg acacagaagc tgaagtattc
     9721 atcgtgaccg tctgcgatga cggtgtcggg atatcagaag aaatctcaga aaaaatattt
     9781 acgctaggtt tttcgacgaa gggaatcaat cgtgggtttg gattgaatct catccattca
     9841 atcgtcaaac gccacggtgg atttttagaa ttttccaaac aaacaaatgg cggtacaatc
     9901 gtctatctag aattaccaaa aaaggaagat caaaatgatg acaaatgtcc tgataatcga
     9961 agatgatcca atggttgcag aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa
    10021 cgtaatcgaa agtttcagaa atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc
    10081 agatctgctg ctgcttgatg tatacatgcc tggcgaaagt ggtatccatt ttttatcttg
    10141 gcttagacag caaggaaatg atatcccagt gattttgatc actgctgcag atgatatcga
    10201 aaccataaag aatgtgatcc gctacggtgc cttagattat ttgatcaaac cgttcacttt
    10261 gcaacgtttt cagctggcga tcgaaaagta tcttgctttc tctcagacaa tccaaacgac
    10321 tgaaaaagcc gatcagaaat tgatcgatca agtattcaac cacaccactc ccattcacga
    10381 aataccatca ttcatcgatt tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag
    10441 tgcatttatt ctaaaagaag atactatttt ttcaacaaaa gaactggctc aaaaaagctc
    10501 actgtcacgt atatccgtca agaaatattt gaatttttta gtagaacaag atatattgaa
    10561 cgaaagaatc aaatatttgg aaatcggccg gccgctaacc tgttatgaat tgacaaagaa
    10621 tgggcggaaa aagctcacag attactgttg agtcatcgaa ttatcccata caaaatagtc
    10681 caagtataca gaagtaacaa ctgtttactt ggactatttt taaacatttt attgacgaat
    10741 atccatgttt tcttatctta attgacgggc tttaggaaga gatacctcat ttttcgtttt
    10801 tccagtttct attatttcac gaaagttctc gaaacttgtc gaaatcatat ttgataatgc
    10861 ctcatctgta tttgaaccta catgcggggt tacaagaact ctagggtaca tttctactaa
    10921 ttgctggata gcagggtctg gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc
    10981 tgtttcattt gcaaaaacat cggttgcaaa accagctaaa tgattgcttt tcaaagctct
    11041 taaaatagct tcattatctt gaagctctcc acgggctgta ttaatcaaga tgctaccttt
    11101 tttcatttga gctaaaaatc cttcattgat catttgatcg ttttcacctg ggatatgagg
    11161 tacatgaata ctgacaatat cgcttttggc taataactct tcaagcgagt ggaacgtcaa
    11221 tactttttta gcagcttctg tctgatgaat atcgtagccg atcactttgg ctcctagtcc
    11281 cttaaacaaa acagcttccg tcaaccctat cctgcctgtt cctatgatcc ctactgtgca
    11341 atttctgatt tcacgactaa acatttgctc atccactcga aaatctttga aagatgttcg
    11401 gatcgtcgta tacgcagtat ttctcaaaag catcatggca aaagtcaaag ataactctgc
    11461 tatcgcattt ggcgaatacg atggtacacg ggctactgtc ataccaaaat cagctgccgc
    11521 ttgtagatcg atatgattga ctcccacagt ccgtgtaaaa acataacgaa tcccatattc
    11581 ctgcattttt gcaagattgg tctcatctgc tatacaattt cccctcaaca atacagcatc
    11641 atggtccttt gctgacacga tattgtcatg tgtcaacagt tcttcataaa gactgagatc
    11701 ataatggtaa atattcaatt tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca
    11761 tgcgattctc atactttctt cctccatttc ttatgaaaat aaacctatgc catgaatcat
    11821 ttgtaataca acgatccata caggtgtcag taccacagca aaaatagttg acagtaagga
    11881 tgcattggag gctaaaagtg cctccctatc aaaactgata gcataggctg ctgccactgt
    11941 tgcagctggt gtagccatca tgataacgat tgttgccagt gcttcaaaat tgactggtag
    12001 aatattcgtt gccgtcaaaa ctgctagaat cacgatattc aatgcaggta caaataccac
    12061 tttgactaga ctgtaatacc atgaagtttt ttcagatgca gcttttttga agcttacttc
    12121 acctaatgtt gcgccgattg ctagccacgc caatggtgaa cttaaaccag ctaaataatc
    12181 catcggctga aatagccacg gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat
    12241 ctctccttct gcacctgtaa ctgacacttg tggcagatac ccttggaaca gccaaatgaa
    12301 aaggccggca aaagtagcga taatgattgg attgacgaac atcgctttga tatttttagc
    12361 ttccatcttc aagccactca tttttatgta tccataagaa tacaaaaaga ttcggtagcc
    12421 aatattgaaa attgaggaat acatcacgcc gacaggacca taaatcgcac tgacgatagg
    12481 cgttccgaaa aaggtcgtag aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc
    12541 ccctttatag cttaagaaaa aaggttttga aactagaata agaataatat aaataactaa
    12601 tccccaaatc aagacattga ttccttgcat cagtaattct gtattgatat cttgcatgaa
    12661 agctcgaaat gccagtgctg gcaaagccac tgttaagacg actttcgata gaatttttcc
    12721 aacagattca gaaaaaatcc ccttttttct acaaacaaac cctaataata taataaaaac
    12781 tgttgaagtg attgcactga caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa
    12841 taagttcatc tttctcctcc taattatgtt catttattca caataaaaaa agcttttatt
    12901 ttgctaatac gtattaacat ttttaatttt atctttacat tgttacttat tgaacaatag
    12961 gaaattaaag aaacttttga aacttatgta aagtgatttt tactagaaca
//
LOCUS       DASOUD010000074        12923 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.074, whole genome shotgun sequence.
ACCESSION   DASOUD010000074 DASOUD010000000
VERSION     DASOUD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.074"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..644)
                     /locus_tag="U4A29_001954"
     CDS             complement(21..644)
                     /locus_tag="U4A29_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681074.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(803..2200)
                     /locus_tag="U4A29_001955"
     CDS             complement(803..2200)
                     /locus_tag="U4A29_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO0681075.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2232..3203)
                     /locus_tag="U4A29_001956"
     CDS             complement(2232..3203)
                     /locus_tag="U4A29_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0681076.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3577..4002
                     /locus_tag="U4A29_001957"
     CDS             3577..4002
                     /locus_tag="U4A29_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681077.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            complement(4490..5191)
                     /locus_tag="U4A29_001958"
     CDS             complement(4490..5191)
                     /locus_tag="U4A29_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO0681078.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5207..5647)
                     /locus_tag="U4A29_001959"
     CDS             complement(5207..5647)
                     /locus_tag="U4A29_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEO0681079.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5647..6222)
                     /locus_tag="U4A29_001960"
     CDS             complement(5647..6222)
                     /locus_tag="U4A29_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO0681080.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7069..7548)
                     /locus_tag="U4A29_001961"
                     /pseudo
     CDS             complement(7069..7548)
                     /locus_tag="U4A29_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7707..8408)
                     /locus_tag="U4A29_001962"
     CDS             complement(7707..8408)
                     /locus_tag="U4A29_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO0681081.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8516..9925)
                     /locus_tag="U4A29_001963"
     CDS             complement(8516..9925)
                     /locus_tag="U4A29_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEO0681082.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(9973..11838)
                     /locus_tag="U4A29_001964"
     CDS             complement(9973..11838)
                     /locus_tag="U4A29_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO0681083.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(11959..12810)
                     /locus_tag="U4A29_001965"
     CDS             complement(11959..12810)
                     /locus_tag="U4A29_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO0681084.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         3685 a         2782 c         2104 g         4352 t
ORIGIN      
        1 gatttcacaa cctctgtttt tcattgtcca taggattgat attgtccttg tgatgactca
       61 ttcgtaataa ttgaggcttg attttcttca cttcccgtta tcgtttgtcc actgccagaa
      121 tctgaaggac ttgttcctgt cgactcgttt ttttgagaag agacagttgt tttcttgact
      181 tgttcatttg tctttttcca ttccgctaaa cgatcggcga ccactttttt gtctactttt
      241 tcagaataag cggtgatcac ttttccattt ttcaaacggg ctactttgtc gccatcatga
      301 gtcaaaagca aaacggtcaa atgcttgtct ttaaattgat attgtctgag agaaatcgca
      361 ctaagtacgt catcgatatt aataaattga ttttcttctt ttccttttgg caaatagatg
      421 tccaattcgc ctttttcgta attggcagtt attttatact ccgcccacca ataagttgag
      481 aattcttttt tgaaattatc tactgattct ttgcttgcta cgattttttc tgtttctttc
      541 tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa
      601 aaaccgaata accaaaagag aagaaacggt ctttttttcc tcatgctttc ctccactcct
      661 atttaatttt gactattcta ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa
      721 aaagatcgta aataattgaa cttctttttc acaaaatggt aagaatggag aatttcacgc
      781 atttgctctc aactattctt tcttaagcct caaatccgtt tttatctgat acgtctttga
      841 accagtgacc cgattttttg attgttcttt ttccatcttg tgctaagtct actgaaataa
      901 agccatatcg attcttatat gcattggtcc atgaccagtt atccatacat gtccacatat
      961 gatagcctaa acaattggca ccttcttgaa ttgcttgatg gacatatttc agatgagatt
     1021 ggacaaattc gatccgataa tcatcgtgga tcatgccatc ttctttgata aatcgtgctt
     1081 cgttttctac acccataccg ttttcagaaa taaagcagtc gatatttcca taatgatctc
     1141 gtacattgat caaaatatca tagattcctt gttcatagat ctcccatccg cgataaggat
     1201 tcattttacg accaggcatt tcataattgt cgaaataatc ctcaggcatt ggaccgtttt
     1261 tagtatcgat tggtgtttct tttgctttta cccggcgtgg ctggtaataa ttgataccta
     1321 aaagatctac tgtattttct ttgatgaccg tcaaatcctc tggatggatc acaggcatga
     1381 gatcaagttc ttttactatc tcaatcaatt cacttggaaa ctcgcctttt acagctggat
     1441 caaggaatga acgattgaag aacgcatctg ctatttgcgc tgctttaaca tcttcagggt
     1501 ttgattcgtc tcttggatag cttggtgtca gattcaagat gataccaata acaccacctt
     1561 gctccatttc atggtaagat ttgatggcta acgctgaggc caacgcttca tgatagccaa
     1621 cttggatcgc ttttttcata tctgactctt ctggataatg ccatcgatat aaataacctg
     1681 cttcgactgg aacgattggt tcattatgag taaaccattt ttttactttc gaaccgaaca
     1741 attcaaaaca acttttggca tatttttcat aggcttcaac cgtttctcgg ttcaaccatc
     1801 cgcctttttc ttgcagatac atcggcatat cgaaatgata cagattcaca aagggttcga
     1861 taccgttttc aatcaactca tcaaaataac gatgatagaa acgaacggct tcttcgttga
     1921 ttgttccaga ttcatcaggt atcaagcggc tccattggat agatgttcga aatgaattat
     1981 gcccagtctg tttcatcaac tgtacatcct cttgatactt cgtataaact tgtgaagttt
     2041 tttcaggtcc tacttggtca aagaactttt gcggttcttt tttgtaccaa taatcaaaaa
     2101 tgttctctcc ttttccatca cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag
     2161 aaccccacca aaaatttgct ggaaattgat attttttcat caaagcacct ctttttcttt
     2221 tttagttcgt tttagttgat tccatgtatg atattttcca tcacaattac tttttcattt
     2281 tcttcctctt cttttttgat tcgtttcaac agctgttctg cggcttgttc cccgatttta
     2341 tctgttggtg gtgtaatcgt gattttcgga aggatgctca aatcgttttt atccaaataa
     2401 tcataatgac taaacgcaat atccttcccg attctgatac cttcttgatt gaaacattcg
     2461 atagcaccga tcgacatatt ataattgctg acaaaaatcg ctgttactcc tttttctaat
     2521 aattcttttg ccccataata gccacttttt ctagaatagt ctcctgtaaa gacctctaat
     2581 tggatcgatg gatcattttg taccgcttct tttgctcctg ctaaacgttc tcttgctacg
     2641 tagtctgttt gagtggcagc aataaatccg atttttttat gtccttgttc accatgatga
     2701 gcgatcactt ttgctacact ttctcgatca ttcactaata ttgtatcaat acctgctggt
     2761 ccttctgggc aattcaatgc gataactgga atactcattt ttgtgagcat ttcaaatcca
     2821 gaccactctt cttcggataa gaagacaatc agcccatcga tcacatgctt catcaggtat
     2881 tcaatttttt gttcaatttt ttctggctga tcggcaaacc cgcttaataa taagctatac
     2941 ccattttctt cagctactct ttcgatccct gagacaactt ccgacccaaa acggctggaa
     3001 atcccgttta tcaaaagtcc aatagagaag cttcgattat tcttcaaccc tttagcaatg
     3061 atgttttctt tatagtccaa cgctgaaatc gcttctgcta tttttttcat gttcttttcc
     3121 ttcagttgct gaccattcaa gtaacgggaa actgttccca ctgaaacacc agccatctta
     3181 gccacgtcac ggatcgatgc catacattct cactcctgcc agttacattt gatttctctt
     3241 tcttgttgtt cgattatgat gaccgctttc tttgcatagt gttgcttgtc atcaatatcc
     3301 atataactaa agtcttcagt agagcaactt cctctcacta ttaattatac ttaaacaagt
     3361 atttgaaccg gttcattttt aatatagcat ttttttaaaa gattgcaaca cttttttaaa
     3421 acgctttcct aaaatggcga attttttgcc tttttaaaat tttattctaa aatttactct
     3481 atttgtgggt gttttgtgcg tttttctctt ccatccgttt gaagttttac tttccaatgg
     3541 tataatttct ttatcctatc aaatggggga agtattttga agaaattaat cgtactattt
     3601 attttgtctg cgggactttt gacattgact ggctgtaatt tgtttggcgg ctctgaccaa
     3661 tcctcggatg aaacaaccga ttcttctgtc ttaaaagcgg aaaaagcaga tatcactaac
     3721 agtgtatata cgctaaaagt acgtaaagat atggatttat ccgcgggaca gttgacattt
     3781 acagataaaa aaatggaatg gacacgcacg tatcagacac aagaaactga ttcttctgaa
     3841 tcatctgaag aaaaaacagt gctgacagac atcaagatta ctaccaaaga tgacgaatat
     3901 attatttcag gaaaagaaaa taaaaaagaa gcaaccatca catttaccaa aatcggtaat
     3961 taccgtttga aggatgagga tggcaacatc tacagtctat agtgccgtct gttggatctt
     4021 cttttgtttc tccccttctt ttctgctata gtaaagaagc aagatgattg taaaggagac
     4081 gaaatggatg atcgatagtc ctgattattg tacatgtaat aacaacaaaa atatccatac
     4141 agaatttaat gaccaaggaa gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga
     4201 ttcatttaaa acatttgaat cttatcctgt tcaaaattat gtaaaaagta gaagaagata
     4261 aacaaatcca agcaaaactc actatggctg atttttatga acttcaaact cagcttcttc
     4321 ctttgatcaa gcttcatcag taaaacaaac agtggatcat ttcagtttac aaatcaaaga
     4381 gtaaagattt atttatttca aatatttgaa tataaaataa ccaaacgata gaaaggctta
     4441 ttctacatca cgtagtaatt tttctttccc gtcgtttggt tttttatttt tagcctaatt
     4501 cttgaccttt tgcatgctgg ttatccgtaa aatattttcc agcaggcttt ccttccaatt
     4561 cagctagttt ttcttggatc tgatggatca ccttttttgc atcttctgga ttttctacaa
     4621 aatcgaattt atccccatcc acttgtattt taggacatac atcaaaatct tcataccatt
     4681 gattatagcg ggtgttcaac gtttcataat aggtatataa cgtctcatcg ctttctagct
     4741 gttcatagtc acgcccacgt tttttgatac gttctagcat cgtgtccaaa gaaactttga
     4801 tatggaccat caaatcaggc cgtttttttg gagcaacatc atctaactcg tttagcatcg
     4861 tatctaataa gctatcgtac tgctgtactt cgatatccgt tacccgacct aaatcagcgt
     4921 ttagatggaa caacaggcta tcttcataga tggatcggtc tagcacattg tcatcgttca
     4981 ctaaagcatt tttcattgct aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag
     5041 cgtactgctc aggatttgaa taaaagagcg gcaatacttc gttatcttcg acattttcat
     5101 aaaatggttt agaatcaatc tcttttgcaa gcatatccgt caaactgctc ttacctgcac
     5161 caatcgttcc tgaaatcaaa atcactgaca tacaaccctt cctcttctat aagtactcgc
     5221 ctacgtaagt ttcaacaggc aatgtttcaa aatcatattc acgtacagct tggtctgatt
     5281 ttaaatacgt atggagactt tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta
     5341 caatgatcat cggttttttc aacatagttg ccacacctaa ttcgtaagcc gttcctggat
     5401 cgattgtttg atgctcaaag tcaaggatag caacaacaaa ttctgcattt gtcagttctt
     5461 ccatatcttt ttcgtatact tcctgcgccc atccagcgct gaatagttca taattgcttt
     5521 cttggtgatg acgtggagag tagaaacttg tgactgtttt gttttcctcc aacgcttttt
     5581 cgatacgact tactcgatcg atttgctcct ctgaaaaaaa tggtcctgct aaatatattt
     5641 gactcattat tttgcctcct tcaataataa ctgattttta atagatactg ctcgtaattc
     5701 attgaccagt tccaatggat tgttggaaaa gattacttga tccaaccatt ttttatccat
     5761 aaaatcctct tttgcaaaag tttctaataa ttgttgcaag ccgctgtaat aaccgccaat
     5821 attcaatatt ccgattggct tgtcgggcgt cagaccgatt gctatccaag aaagaagttg
     5881 tgagaattcc tccaatgtac ctagacctcc agggaaaacg aggacagcat ctcccttttc
     5941 aaataataat acctttcttt catcgatcgt ttctgtagga ataaatgtag taacatcttt
     6001 tccaggcaat tcagtttcaa aaagcccatt cggatagata cctgtgactt ttccgttatt
     6061 ttccagtact gttttactaa tcactcccat aatcccggag tatgaaccac cgtaaaccag
     6121 ttcgatttct tcttgtgcca tgtattttcc taacgtttgc gcaataaatt tgtaactttc
     6181 tttattacca aaacgggaac cgcaaaaaac agtaagtttc ataaaaaaac actccctaac
     6241 tcaaaagtct atcttcattc tacacgtgtt tttttaaaat aaaagactag attttgtagt
     6301 tttttagtgg tatataggat agacaccact atatatagta tttagaaaaa aacatttttc
     6361 agctatttta tagataaaac aaaaataaat aacgtgtttt catcgatgtg aaatgactta
     6421 cttatagaag atactgggat ttcgtcctat atgccaataa cgtgtattat tttaaacaac
     6481 aaatacttgt ttcttttatt aacgaaaaaa tcattttgtt ctcgtgttat gtttcactga
     6541 taaatgatgt ttctctaaat aaaaaagccg acgaactaaa cgatcaaaat ttgatcattt
     6601 ttgttcatca gcttctccta aaaatctttt tgttaacgat aaatagctgg atttgtaaaa
     6661 atatcacttg aatcgtcact gctggaagaa aattcagtga taaccgcacc tgttgcgcct
     6721 gcctggaact agtgtttgat attgggctcg aacgtgtact gatctctagc atgaagaata
     6781 atttcttttt ttactgtata ataactttct ttcctttttg gaatagtaca ggaaagtttt
     6841 tttgtttcct ttccttcgac ataaggtata ccagaccctt tcgacaacga aaggttctca
     6901 tttttccttc atcgtcattt caaggagggt gccgatgctc tggacagttt cgatcgctcc
     6961 taaaccaaaa tctgtatatt cgattctttc taactctgat tctttcatac cattcctgcc
     7021 tcaaggtaca tctattgtac ttttttcttg taatccactc tgcctctcct aactaattgt
     7081 caaatcatcc caagatatgt cttcttcctc tttttcttct tggatgattt ctgcattcgg
     7141 atctactggt tttttcaata ataaaatggt tattcctgtg acaaaagaac cagctaacaa
     7201 tgcaccaatg aatgcccatg gtctggtcat cgtagcgact gcaaacattc ccgatgccgg
     7261 aatacggcta tctgcgcccc aaaacatgct taatccgcca cctactgtcc caccgactcc
     7321 cgttgctatg acattgcgtg ccaagtcgtt caatacgata gggaaacatc cctctgtgat
     7381 ctgacagatc cccataggaa aagcggtctt caatgtatcg atttcttgac gattgagtat
     7441 atttttacga atgattttcc ctaagaaata agcgatcgtc aagccgaatg gggttaccat
     7501 cgaagctaaa atcaatacag tgattggttc atagattcct tctgaaaatc gtgccacacc
     7561 cccttatcca gaataaatgg taatccagaa acagccgtgt atctgcggta ataagcctaa
     7621 ccaaacgaaa aatatgagaa gctatttttg tttggctgtt cttattactt gatgcagggc
     7681 agctttttca caacctcttt ttttatttat gtttcataat caatatggta aggatgcagc
     7741 tccgtatggt agaccgcctc cgaatattca atcatttccc caatttgcgt agacgatttc
     7801 cgtatccgtt tgatgccgat ctgtgcgtct gttttcagaa ttccttgttc ttcttctgtt
     7861 aaaaatactg ccgagaaatc atcagaaaat gagtcaatag tatatccttt tttcgtcagc
     7921 aaacgataca aagattcttt gccaaattct tctctgctga attgcatcat ttcttgtgga
     7981 agataatgaa caaaataaat ataaggctga ttgttcaaat aatataagcg cttaaaacaa
     8041 acagcttgct caccgaaaaa ggaaaacaaa ggatcttcct tctttatatg gaccaattgc
     8101 aaatccagcg tttctttccg gacttcatat cgggagttct ccaagatttg cgtaaagctt
     8161 gccgctttgg ataatcgatt gtaaggacga ttgcttagaa ccgtcgtccc acgaccactt
     8221 ttctttgcga catattcatc tgatgccagc agttcgattg cctttcggac agtgattttg
     8281 ctgacttgaa acagttcttc taattcactt tctgtcggca acatcgaacc gactggatat
     8341 tttcctgaga aaatatcttc tttaatttta tctgcgactt ccaagtatag tacacgtgtc
     8401 cttttcataa cgtcaccctt tgctttacta ttactaaaag aatagcacat aaaaaataaa
     8461 aaaagaacac aaatcacatg tgagaagtgt gttctttttc attcagctgc tttaattaca
     8521 agtttacgcc atttgaggca attacttctt tataccaatc aaaagatttt ttctttgatc
     8581 gattcagtgt cccatttcct tcatcgtcta gatccacata gataaagccg taacgtttac
     8641 tcatttctcc tgttgatgca ctgacaagat cgatacatcc ccaaggcgta tagcccatca
     8701 aatctacacc gtctgctact gcattagcca tcgcttcgat atgacgacgt aaataatcga
     8761 tccgataatc atcaataatc gtgccatcct cttccatatg atcaatggct ccaagaccgt
     8821 tttcaacaat gaataatggt ttctggtaac gattgtataa ttcattcaat gcgattctca
     8881 gcccttctgg atcgatctgc cagccccatt cacttgcctc aagaaacgga ttttttactc
     8941 ctcccagcaa gttcccttct gaagttgctg cttgtggatc agtctcattg attgccgtag
     9001 acatataata actaaatccg atgtagtcca ctggatattc ttttagcaac tggaagtctt
     9061 tttccgtttg ctctaatttt tccggatcga ctccatattt tttatgggta cgcgctgtgt
     9121 atgcagggta ttctccacgt acttggacat ccgtgcagaa aaaattgaat tcttggtttt
     9181 ggatcattgt tgctgcctga ttgactggat tggcatcgat gctgtaagtt gttgcataga
     9241 taatcatgca gccgatttgg atatccgaac gcaattcgtg gccgattttt acggctttgc
     9301 tacttgcaac gaattgatta tgccatgctt ggaaaatatt ttggtactct cctgctccat
     9361 tgctttttac taatccttga cttaaagctg gaaagtgaaa ggccgaattg atttcattga
     9421 aagtcatcca atatttgact ttactgctgt atctctctaa tactgtctga gcaaatcgtt
     9481 catagaagtc gatcagtttt cgatttttcc agccaccgta ttcttttgcc aaatgaagag
     9541 gcatttcgta atgagaaatc gtgatcacag gttcaatatc atattttaag cactcatcaa
     9601 tcagctgatc ataaaattct aatcccgctt cgttaggagt accttcgtct ccatttggaa
     9661 aaattcgtgt ccaggcaatc gaaaaacgat aacatttgaa tcccatttcc gcaaacaagg
     9721 caagatcttc tttgaaacga tcgtaatgat cgattcctcg atgattcggg taaatatatt
     9781 tttcttgatc aatcgtccag tcaaattctt cactgccgat aatcgcaaag cgctgcttac
     9841 ctccaggcat agcatctgca acggatagtc cttttccgtc tcgatcataa gccccttcta
     9901 attgatttgg cagcagttgc tccaccccat aaaaattttt ctgaaaaacg tgattgcatc
     9961 ctgatttcct ccttatttta caattgccag aacatcttcg tttgcaataa tctctttttg
    10021 gtctaattca agtatatcgg caaatgcatc agtattcgta atgacaactg gagtgattac
    10081 gctatagcca gctgctctaa ttgcttcgat gtcaaaacga atcaataaat ctcctgcttt
    10141 gacgagatcc ccttgtttga ctaagatctc aaagccattc ccatttaatt ctactgtatc
    10201 catcccgata tgaatcaaaa tctccgctcc gtcagtcgtt gtgatcccta cagcatgtcc
    10261 agttggaaaa atagtcgtga tctctccgtc tgcaggtgca actagttctc ctacttctgg
    10321 atcaatagcg ataccttttc ccatcacgcc agaagaaaaa acttggtctg gtacttcact
    10381 caatggcagt actttacccg ttaaaggact ttttaaggta tgccgtgctg aagtaatcgg
    10441 ttctcctgca ttcgcatgtt tgttttctaa ttgattgtct tgtgtttgtt cgtcaaatcc
    10501 taaaatcaat gtacctacga atgcaagaac aaaagcaatt cctgtagcta ttactgctac
    10561 tgtcacattt gactctacac catccaccgt attaatgaaa gtcggaatcg tcaataagct
    10621 gaccaaactg ctggagaaag cttttactcc ggaaaatccg ataatagctc caccaatacc
    10681 tccagaaata caagcggcaa tgaatggttt cttcaaaggt aatgtcacac catacacagt
    10741 tggttcagtg attccaaata acgaagtcac tgtagaagaa atccctaatg ctcgaagttt
    10801 cgtatctttt gttcgcaatg ctacagctaa agcagcccca ccttgtgcca agatggctgg
    10861 aagaagcatt ggcatcaata catcaaaccc atattgttcg atattcaaaa acatgatcgg
    10921 tacgaatccc caatgcatgc cgaacatcac gaatacttgc cataaacttc ccatgattag
    10981 acctgctacg attggactaa atccataaat cgagttgaag agaccgccta aagcattacc
    11041 ggcaaccgtc ccgattggtc ctaaagcaag aaatgttaat ggaaccatca ctactaaaac
    11101 gacaagcgga actaaaatca tttgcagaaa ttgcggaatc acttttttta cgaatggctc
    11161 taatttactt tgaacccata cggctagaat gattggtatg acagaagaag tataaccaga
    11221 tggtgaaaga atcactggga tcccaaagaa actgatagct tctcccgcac cagcaactgc
    11281 tgtgatagct ggatacacaa gtgccatagc aattgctact gctaaaaact ggttggcttt
    11341 gaacttttta gctgctgtaa aagcaagcat gacaggtaag aaggtaaaga caccatctgc
    11401 tgcagcaaac aatatttgat aagtaccgct atcagctgtc agccagccca tcaccgttgc
    11461 caatgataag aatcctttca ataccccggc ggctgccatc gcccctagaa aaggtgtaaa
    11521 tatactagag ataatatcga tcagacgatt caaaatattt ttggactctt cttttccatc
    11581 ctccgaaact gcaagacctt cctcatccac gatcgattga taaacatctg ctacattact
    11641 gccaatcact acttgatact gaccgccact ttgtacgact tggatcacgt ccgggtcttc
    11701 ttttaacttt tgcgtatctg ctaaagattc atcttttaat ctgaatcgca accgcgtagc
    11761 acaatgtact aaacttttga cattcttttg tcctcctacc gcttcccata cacgacgacc
    11821 aactgcttga ttatccattg ttttttcctt ctttcttttt tatttaaaaa ataaaaaccc
    11881 agattgaaaa aaacagtcac aaacattcgt gaacctattt tttcaatcca ggttttgcct
    11941 gccatacagt aacaatcctt atgattggat gctttttttc tcacttacta ctctttgaat
    12001 atgaatcgtt aaataaatct gctctgcgat ccccatttcc tgtttttgcg tttgtcgaag
    12061 atactcattg atccgttcga cacaatgaaa cgcttttgga tactttcctt gaactaatgc
    12121 gtagagaaat tcatcttgct cgtcagatcc ttcccctttg agataacgct gggcaaaaaa
    12181 ctgcagatga gtaatgatac gctgataatt caaagattct tcatcgaaca cgatgccgaa
    12241 gaatttactg atgatactca gtatatctct taccattgtg gtagcggtca tcgtcacttg
    12301 catgtcttcg tttccttgtt ggctattcac aaaatgaaag gcaataaaac cggcttcatc
    12361 ctcagaaagt tccacacccg tcttttgttt cacgaacaaa agagcctctt tcgctgcttg
    12421 atattctttc ggataaaatt tttttgtttc aaataataat ggattaggca gttggatatt
    12481 ttcttttgct cgtgtgatcg cataatgaag atgatcggtc aatgtcagat aaatattagg
    12541 cgaaaattca acttttagtt tatcttcagc cagttcgact acttgtttga cccattcgat
    12601 ctcctctgcc ggaatatcag ccatcagctg ctggaattgg tttttagcaa aagaatctct
    12661 taaaacaaat tctttttgga ccgcttctgg cgaaatctcg tccccaatct ttttttgaaa
    12721 agctagtcct tttcccatac aaacgacttc ttccccgaat ccatttttcg aaatgacgac
    12781 attgttattc aaaattttat caattttcat tttgcttcgc cctctcaaga aaaataaaaa
    12841 aacctagaat tcgcctactg tttcttagct gaattctggt tttgcctgcc gtacagtaac
    12901 aatccatttc ttattcaaaa tca
//
LOCUS       DASOUD010000075        12670 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.075, whole genome shotgun sequence.
ACCESSION   DASOUD010000075 DASOUD010000000
VERSION     DASOUD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.075"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="U4A29_001966"
     CDS             6..305
                     /locus_tag="U4A29_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0681085.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U4A29_001967"
     CDS             378..1739
                     /locus_tag="U4A29_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO0681086.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U4A29_001968"
     CDS             1761..2021
                     /locus_tag="U4A29_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681087.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U4A29_001969"
     CDS             2035..2787
                     /locus_tag="U4A29_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEO0681088.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U4A29_001970"
     CDS             complement(2881..3486)
                     /locus_tag="U4A29_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO0681089.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U4A29_001971"
     CDS             complement(3539..4513)
                     /locus_tag="U4A29_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEO0681090.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U4A29_001972"
     CDS             complement(4891..5610)
                     /locus_tag="U4A29_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO0681091.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4A29_001973"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4A29_001973"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEO0681092.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="U4A29_001974"
     CDS             7161..8558
                     /locus_tag="U4A29_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO0681093.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="U4A29_001975"
     CDS             8639..9079
                     /locus_tag="U4A29_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO0681094.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="U4A29_001976"
     CDS             9227..9553
                     /locus_tag="U4A29_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO0681095.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="U4A29_001977"
     CDS             9554..10303
                     /locus_tag="U4A29_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681096.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="U4A29_001978"
     CDS             complement(10383..10838)
                     /locus_tag="U4A29_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO0681097.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="U4A29_001979"
     CDS             complement(11265..11540)
                     /locus_tag="U4A29_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681098.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="U4A29_001980"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="U4A29_001980"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO0681099.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2175 c         2587 g         3852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DASOUD010000076        12129 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.076, whole genome shotgun sequence.
ACCESSION   DASOUD010000076 DASOUD010000000
VERSION     DASOUD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.076"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..362
                     /locus_tag="U4A29_001981"
     CDS             6..362
                     /locus_tag="U4A29_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HEO0681100.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            378..728
                     /locus_tag="U4A29_001982"
     CDS             378..728
                     /locus_tag="U4A29_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HEO0681101.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            757..1536
                     /locus_tag="U4A29_001983"
     CDS             757..1536
                     /locus_tag="U4A29_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HEO0681102.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1547..2224
                     /locus_tag="U4A29_001984"
     CDS             1547..2224
                     /locus_tag="U4A29_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HEO0681103.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2243..3349
                     /locus_tag="U4A29_001985"
     CDS             2243..3349
                     /locus_tag="U4A29_001985"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEO0681104.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3339..4430
                     /locus_tag="U4A29_001986"
     CDS             3339..4430
                     /locus_tag="U4A29_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HEO0681105.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4443..5183
                     /locus_tag="U4A29_001987"
     CDS             4443..5183
                     /locus_tag="U4A29_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HEO0681106.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5471..7411
                     /locus_tag="U4A29_001988"
     CDS             5471..7411
                     /locus_tag="U4A29_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HEO0681107.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7498..9420
                     /locus_tag="U4A29_001989"
     CDS             7498..9420
                     /locus_tag="U4A29_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO0681108.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9700..10122)
                     /gene="arsC"
                     /locus_tag="U4A29_001990"
     CDS             complement(9700..10122)
                     /gene="arsC"
                     /locus_tag="U4A29_001990"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HEO0681109.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            10514..11536
                     /locus_tag="U4A29_001991"
     CDS             10514..11536
                     /locus_tag="U4A29_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO0681110.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            complement(11661..12095)
                     /locus_tag="U4A29_001992"
     CDS             complement(11661..12095)
                     /locus_tag="U4A29_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO0681111.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
BASE COUNT         3940 a         2050 c         2638 g         3501 t
ORIGIN      
        1 caatcatgat taaagtagtt attgcagacc gtatgggtaa aggacaaaat gtggccaaag
       61 gggtagaagc agctggtgga aaagcagttg tcgtaccagg aatgggggca gatatgcgtt
      121 tgggcgatgt gatgcaacaa gagcatgcag atatgggtat ctctttttgt ggtagtggtg
      181 gtgcaggtgc attaacagca gcaacaaaat atggctatcc agaacgacat ggtatgcgtt
      241 cgattgaaga agggatcaca gctatcaagg atggcaaaac tgttctaggt ttcggcttta
      301 tggatcaaga ggaattaggg aaacgtctga cagaagaatt tatcaaaaag catggaaaat
      361 agaaagaggg acatgaaatg agcgttcaaa agacaattca gcaaaaagtt atcgtctccg
      421 gtaaagggga aacaaagcaa catgcttttg catcagcgtt agcagaaatt caaaacaaaa
      481 taatggaaca atcaaacgta ttattgcgaa tcgaaccagt caatgtggat attttaaagg
      541 cggtcgaaaa caaatacaca gaacgctttc tatttttctt cttcccacgg caacgtatta
      601 gctatgaagt cgaactctct gttactgtgg ctgtaacaga aataaaattg gcagacatat
      661 cgttcagcca gcaaatgact gaagatccaa atgggattgc cattccattt gtaactaaaa
      721 aaatctaatt ttgaatcata agggggaaag aataacgtgg atttcgtaat cattctagtc
      781 aaatcggtca ttatcggggg attacttggt tttgcagcat ctgtcggggc agctcgaatg
      841 tttcatgcac ctaatacaca aggtttagga gcttttagaa cattaggcga aatgaatgcc
      901 tgcatgggtg atccagcttc tcatttttca tttggtttag gctttttctt taatgcatgg
      961 gcttcttcag ttggtgctgg tgcattcaca caagatgtga cgcatcgaat cattcccaat
     1021 tgggcagcgg cagcacttct agcaaaaaac aaagatgtca cccagaccat gcatgatccg
     1081 aaaaaaatgg ggatcattgg agcggttatc ggggcagtgg tggtagcctt cttaaacaca
     1141 acagctgcag caattccaga ggcgttacaa gtgacggcgg tttccgtttt ggtaccagca
     1201 gcgacgattc tgatcaatac agtaatgcca gttatttttt ggttggcagc tttagacgca
     1261 gggaaacgaa ctggtttttg gggcactctt tttggtggta tcgcgcaatt gattatggga
     1321 aatgcggtac ctggtgttgt tctaggaatc ttgattggta aaggcgtcga tgagagcggt
     1381 tggaatcgcg tcacgaagag catgatgatc gctattgttt tattatttat tttcagtgga
     1441 tttttccgtg gatttgatat ggaaatgatc aagagcttcc agcttcaagt gcctaattgg
     1501 cttgataact tgcacaacat ctttagtgtg aaataggagg agaatcatgg agacaacaga
     1561 agaacaagca aaagtattag aagcaaagcg gcaaaagagc ttttggtttg cagactggtc
     1621 ttttccgcta atcgtaggaa ttatgtccgc tgctgtattt gcaggaacac acatgtatgt
     1681 gatgtatggt gtgggtgcat tcaatgaagt ttcgatcgtg gcgatgttaa aagccgggct
     1741 ggatggcgga tcctatggcg cagcggcagc gtttggtgct agtttcttat ttgccagaat
     1801 tttagaaggc tctttagttg ggatcttgga cttaggcggt tcgatcctaa ctgggatcgg
     1861 aattggggta ccagcaattc tcttaagtat gaaaatagcg gcaccaatcg ataattttgc
     1921 actctcttta ttgactggtt tggttctagg tttgattgtt ggtggcatca ttgtcttgat
     1981 tcgtaaattt acgatcaatc aatcaaattc cacttttggt gctgacgtca tgatgggggc
     2041 aggaaattca tctggacgtt ttttgggtcc attaattata ttaagcgcgt gtgcagcatc
     2101 tattccaatc ggcattggcg ctacattagg agcactagga ttctatgctt ggaaaaaacc
     2161 aattgctgga ggtgctattt taggagcaat gattttagga gcaatcttcc cggcagtaaa
     2221 ttaatagaaa gagggtcatt aaatgtttga tactttgatt aaaaatggaa ggttagttga
     2281 tggatctaaa atagaggtcg ctatagaaaa aggcatgatt aaagcagttg cagcagaaat
     2341 aaatcagccg gcgcaagaag tagttgactt ggagggaaag tattatttat ccgctgggtg
     2401 gatcgatgat catgtccact gctatgaaaa gatgaatctt tattatgact atcctgatca
     2461 gataggggtc gaaaaaggtg tgacgacggt cattgatgct ggaactacgg gagcagaaaa
     2521 catccgtgaa ttttatcaat tgacgaaatc ggtcaagaca aatgtgtatg cgctaatgaa
     2581 tatctctaaa tggggcattg tcaaacaaga tgaacttgct gatctaacaa agattcaaga
     2641 agatctagta agtactgcgt tagcagaact gccggaattt attgtcggga ttaaagcgag
     2701 aatgagtaag acagttgttg gtgaaaatgg cattaagcca ttagaattag ctaaaaaaat
     2761 ccagacagaa aacaatgatc tgccattgat ggttcatatc ggttctgcac ctcctgagtt
     2821 agaagaagta ttgtcaagaa tggaaagagg cgatgtattg acccattgtt ttaatggaaa
     2881 accaaacggt attttagatc caaagacaga tcaaattaaa aactttgtct gggatgctta
     2941 caataatggt gtcgtctttg atattggaca tgggacggat agctttaatt tccatgtggc
     3001 agaaaccgca ctaaaagcag gaatgaaagc tacctctatt agtacggata tctacatccg
     3061 caatcgtaca aatggcccag tttttgatct agcaactact ttagaaaaat tgagggtagt
     3121 cggctattct tgggaagaaa tcattgaaaa agttacagca gttcctgcac ggaattttca
     3181 tttgaagaat aagggccagt tagctgtcaa ttatgatgca gatttgacga tatttgagat
     3241 tgttgaaaat gaaaaggaat tgaaagattc caacggattt acacgtatag ctaaagaaca
     3301 aattttaccg attaaaacaa ttattggagg cgtcatatat gacaattagc tatggaaagt
     3361 ttgatttaaa agaagtcatt aatgcatcgg gaaaaatgac gatcctaggg gtttctaagg
     3421 tatctgaaag tgtcctagca gcacaacgat ttggaggcga gcattttttt gagatgagtg
     3481 atcttgccat caaaaccggc gcttatcttt ctcaattact gaaagtggaa gatgcgcaaa
     3541 tcgtttcttc tgcttcagca gggattgctc aatctgttgc tgctttaatc ggacagggcg
     3601 acatgtacca tgtctatcat ccctatacag aaaaaatcgc tagacgggaa atcatcatac
     3661 ctaagggaca taatgtagat tatgggacac cggttgaagt aatggttgct caaggtggag
     3721 gaaaagtcat agaagctggc tatgcaaata tgtgtacacc ggaacatgtt tccatgatga
     3781 ttacggaaaa aactgctgcg cttctatata ttaaaagtca tcatgcagtt cagaaaagta
     3841 tgctgacggt ggcagaaatg gtagaagttg ccaaagctca tcgtttgcca ttgatcgttg
     3901 atgcagctgc ggaagaagat ttattcaaat atagcgaaat gggcgttgac ttagtgattt
     3961 actctggggc aaaagcaatc gaaggtccga gtgccggact ggtcatagga aaaaagaagt
     4021 atattcaatg gattcggcta cagtctaaag gtattggccg agcaatgaaa atcggtaaag
     4081 ataatatttt aggctttacc caggcactag aagattattt aaaaaatggg agcgaatccg
     4141 gtgactccat gaagcaacga ttagcgcctt ttgttaacgc aatcaatcaa cttcctgacc
     4201 tttcagcgaa aatagttcaa gatggtgccg gcagagatat ttatcgagca agtgtaacga
     4261 ttaatggcga taaaagtgcg aaagaagtca ttcaggaatt aaaagcacga aatccagcag
     4321 tctacacccg agaatatcaa gcaaataacg gaatcattga gttcgacatt cgttccgtca
     4381 gccaaaacga aatggaaaaa attataaaaa gattgagaga aatcatgtag gaggctttta
     4441 aattgtcagt tatcccaaat tattataaaa atcgtgtatg tttaaatgta ttagctcatt
     4501 cagttgaaaa tgcaaaagca tgctatgaag cagcggaagg gcatgtcgta ctaggtgtgc
     4561 tatcgaaaaa ttatgctagc gatgaagcag caatcgaaga tatgaaaagg taccaagacg
     4621 caaccaataa tgcgttgtca gttggattag gcgctggaga tccaaaccaa agtcagatgg
     4681 tttcgcgttt atctggggtt ttacaaccac aacatgtcaa tcaagtattt actggcgtgg
     4741 ggacttctcg tgctttattg ggccaaaatg atacagtcat caatggattg gtttcaccaa
     4801 caggtaagat cggctatgtc aatattgcta caggtccgtt aagttcacaa gcgccagcag
     4861 ctgaagtgcc tattgaaact gccattcaaa tgctgcaaga catgggaggt acttcaatca
     4921 agtatttccc gatgaaaggc ttagcacata gggaagaatt taaagcagtt gcggaagctt
     4981 gcgcaaaata tgaattctat ttagagccaa caggaggaat tgatctttca aactttgaag
     5041 aaattctgaa aattgcaatc gatgcaggtg taaaaaaagt gattcctcat gtttatagtt
     5101 caattataga taaagaaaca ggagatacgc gtataggaga cgtaaagata ctattaagca
     5161 tggtaaaaaa aattattcac taactcatta gaaaataaga agcttattcc agaatgaaat
     5221 taaatggaat aagcttttta taattaatta cggaaattgt taatgtagat aataatttag
     5281 atgtacgtaa gcagaagtac agaaatgtaa gattttccaa tattatttta ttcaacataa
     5341 taaatcgtat atcaattttt ttagcgaaat ttctatttat tccaagaatt tcagttatta
     5401 cggaatcatt ttgtgcttaa taaaaagaaa ccgtttttat atactataaa tgtagaaagg
     5461 cggtaggaaa atgaaaaaaa ttattgcaat cacatcctgt ccagttggta ttgcccatac
     5521 atacatggca gcagaaaact tagagaaagc ggggaaagca gttggcgccg aggtaaaagt
     5581 cgaaacacat ggttccatcg gtattgaaaa tgaattgact gcaagagata tcgaagaagc
     5641 agctggtgta atcatcgcag cagatactaa aatagataaa tcacgattcg gcggtaaacc
     5701 tttgataacc gtaggtgtgc aagaaggaat ccaccatgca gatgagttgg tgagagatat
     5761 tttagcagga aaagcgccag tctataaaag tacggaggca attatttctt ccgaaaataa
     5821 aagtgaatca gaaaatttag gtaaaaaaat ctataagagt ttgatgaatg gcgtttcgta
     5881 catggtacct ttcgtagtta caggagggtt attgatcgcg atttctttaa cattaggagg
     5941 aacagctaca cctgaaggga tcaagatacc agaaggaacg atttgggcaa cgatgaatag
     6001 tatcggaagt atcgctatgg ggttgatggt tccaattcta tctgcattta ttgcgcaaag
     6061 tattgcagat cgtccaggac ttgttcctgg atttgttggt gggatgttag cggcaaacgg
     6121 agcattatat ggtagtgatg cgaatgcggg attcttaggc gggatcatca ctggattttt
     6181 agcaggattc attgctttag gaatcaagaa agtgcgagta ccgaaagctt tacaatcgat
     6241 catgccaatt attgttatcc caattttagg atcattggca gttggttttg tgtttatcta
     6301 tgttattgga gaaccagtag ctcttctatt tagttcattg actcactttt tagcagggat
     6361 gcaaggagga agtgaaattg ttctagctat gatcttagga gcgatgattg cttttgatat
     6421 gggggggccg ttcaataaag tagccttttt gttcggatct ggcttgattg cagaaggcaa
     6481 ttattccatt atggggccaa ttgctgtagc tatctgtatc cctccattag cattggggct
     6541 ggcatcattg attttaaaaa ccaaatttac gaaaacagaa cgagaagctg gtaaggcgtc
     6601 cttagcaatg ggactgtttg ggattacaga aggcgctatt ccgtttgctt caacagatcc
     6661 actacgggtg attccaagca ttgtaatcgg ggcaatgaca ggaagtgtga tcgcgatgct
     6721 ttcaggagtg acagatcatg tagctcatgg cggtccgatc gtagcggttt taggagcagt
     6781 ggatcacgta gccatgtttt ttgtagcagt gattgctggt attgctgtaa ccgtgctaat
     6841 gttaagaatc cttaaaccga cattacaaca aactgcggtt gctgcagcaa atggtgtgac
     6901 agtagcagat ctatcagaga ctgaaacaag tgatgtttat acgaaaactg aaacttttga
     6961 ttctatcacg gatattatgt cagatgaagc aatcgtttta ggtgaaacag caacaacaaa
     7021 agacggagta atcgaagatt tagtccaacg tttagaaaaa acagcggcga tcacagataa
     7081 aaccagattt aaagaagtca tctatgatag agaacatgaa agtacaacgg gaatcggtat
     7141 ggggattgct attccacatg gaaaatcagc ttatgttctt aaaccaacag tggcatttgc
     7201 cagaagtcaa tctggggtag attggcattc tttagatgga aaacctgcac atatgatctt
     7261 tatgattgct gtacctgaaa acagccaagg tgatatgcac ttgaaaatac tgcaacgtct
     7321 atcacgtaaa ttgatggatg atgacttccg tcaagcactg atggaagcgc cagataaaac
     7381 aacagttcac caattattaa gcgaaatgta acataagatg aaaaatcgac cgtgatgaga
     7441 cataatagat gtgttatcat gatcgatttt ttatttagaa gggagtttct gcacattatg
     7501 aatcagcgtc agcaatcttt gatctatcaa ttaagtgaga acaataatta tataacaaca
     7561 aaaaagctag cagagcattt ccatgtgtca gaaagaacga ttcatagcga tttgaatacc
     7621 attgaaactt ggttgagaga caaacatctg cctttaatgt ttgaaagaaa aaaaggtaca
     7681 ggtatcttgt tgaatggtct gcctcacgaa agggaacaac tgaaacgagc gcttaatgaa
     7741 gagactcaaa cagaaatgga taaaaaacag cttcgtcagt tattgatttt ccatctgcta
     7801 gtagcaaaag acggatttag tcttgaagaa ctttcagaaa aactatatgt tggaaaacgt
     7861 acgattcgta aagaattgac agagttgcaa agcttttttg agtatcacca tttacagcta
     7921 gtatccaaaa caaaactagg aacttttctt aaaggcgatg aacaagaaaa aagacagttg
     7981 cttgttaaga cattacgaaa tatgcagaaa gaagatccac aaagtccgtc gttgaaagaa
     8041 ttcttcgcta aagatacatt aaaagttatc caacatacat tacaagatgt tttttatgaa
     8101 aaccatttag aacagccgaa tggactggct agtgtggaga ttcacatcta ctttatgctc
     8161 gagcgaatga aacagtacca gaaagtgaaa ttaagtaaag acgaaaacga agtcgtggaa
     8221 cacacccaag cacaaaagtt aagtagtcaa atattagcaa aactagccac catctatcca
     8281 attgaatttt ctcctgatga aatcaattat ctggctttgc gtatcgccaa tctttttaca
     8341 aattcacaag cagccacacg ttttcaaaag gaatctagcg cacttgctga tcatttgatc
     8401 gaacaagtgg aacagtttct aggctattca ttaaaagaag atcagctact aaaacaaaat
     8461 ttaaggtctc atttatcttc aacttatttt cgactccatt atggtttgca gatttctaat
     8521 ccattgacga aaaatgtgtt tagtacgtac acacaacttt tcttagtttt acagttaatt
     8581 ttagaagatt attttgaaaa ggaaaatttt tatgttccac aagaggagac agcttattta
     8641 acgatccatt tccaagcagc gatcgagcga cacaaacatc agaaaagtag aagatatcaa
     8701 acggttttag ttagtgaata ttcaaaagct atggctactt ttctcgaagc acggctgaac
     8761 agagaacttc cagaattaac gattatggat ctagttgaat ataagccaga attaaagaga
     8821 caagattttc cctctgttga ttttatcttg gctacgttac catttaaaca tataagcatt
     8881 ccagtaatcg agatctcacc aatgatcaca gaaactgatc tagcctattt gacaaaatat
     8941 atgctggagc atgtgcctat caagaagaaa aaaacatttg atctagcaag ctttactcat
     9001 ccatttctga tctttcctca acttgaacgg acagacccag ccgatatttt aaactttatg
     9061 ggaaacgtct tagtggaaca tcattatgtg gagccggaat tcatcgattc tgtcttggaa
     9121 agagacagtc actccagtac tagagtagca ccatttgtaa caatccctca cggtaatccg
     9181 ctgtatgtga agcattcgat gatttctatt gctacaatga aagaaccagt cctatggcac
     9241 ggggaacaga tacgtatcgt actgctatta gcaataaaga aaaatgattt gaaacatgca
     9301 gagttcaaaa aaatattttc tgttattcac tatctagaaa agcagccaga acaaatggct
     9361 cagttgatgc aatccaatca tccgctagaa atcttaacgt tgttatctag ttatgaataa
     9421 gtagcgatta aaaaactgcc tttatattta taataattct atctatttat ttggaagtag
     9481 aaaactaaat acaaaaaaag aggatgtggc caaagttgtg gcacatcctt ttattttgag
     9541 tttcttataa ttttttatag ttgcttacga tgtcttgact actggtaaac ggtgttcaag
     9601 agacaatttg tcggcaataa ggcacaattt tgtaaaatat ggaaagctat ttttttaaca
     9661 ttgtttctta gtgcttgaaa aatcattttg aatacttctt tatacgatcg atgtacgttg
     9721 atttaagtct aaaacttttc ttttgattcc ttctctgact tttctgaatt ccgttaatat
     9781 ttcttcttcg ctcccgacag cttgtgcagg atcttttagg tcccagtgaa ggtgttcgtg
     9841 attgtttgga acggcagggc acttgtcttt ggcatcacca caaagtgtaa tgatcaagtc
     9901 tgcctgagta aagtaatctc tatcaatcaa atcagacgtc tgtttggaaa tatcgatgcc
     9961 gtcttctgcc atgattttta tagctcttgg attcaagcca tgtgtctcga tccccgcact
    10021 tcttatctca aacattgaag gagacaagta tttacgcgcg tagccttctg ctatttgact
    10081 gcggcatgaa ttgcccgtac ataagaaata gatttttttc atttgatttc ctccgttatg
    10141 gattagtaaa tgtcactgtt tatctaaagt aaactctatg tgatggagcg aaaaaacaat
    10201 gggtttctta gtttatatat ttctttttgt tcagcgtata aatcgatgtg tacaaagaac
    10261 taatcttgtc ttttttacca taaatacatg ttagaaacaa tcattttgag tcaaaaatca
    10321 acgatgtaac agactgttaa aagagattaa gacacggcag atgatgaaga aagtaggggt
    10381 gtagtaagcc gatacatttc ttgattgtga ggcgagaaca attattcgtc ttttaagaaa
    10441 aaaagtagta gaatcagttc atattaaaaa taaaaaacct tccatttttg taggtttaaa
    10501 ggaggtcatt accttgaaaa aaatcggctt tttaagcttt ggacattgga tggagcaagg
    10561 atcgcttgtg aaatcagctc aagatgctta tttgcaatcg attgatttag cggtggaagc
    10621 agaaaaaatc ggagtggacg gagcgttttt ccgcgtacat cattttgccc ctcagattgg
    10681 gtcgccgttt cctttgctag cggcaatcgg cgcaaagaca agtaagattg aaatcggtac
    10741 aggacttatt gatatgcgat atgaaaaccc ttattatatg gcagaaaatg ccggattgac
    10801 agatatcatc acgcaaggaa gagtgcaact aggagtgggg cgtggttcac ctgaacaagt
    10861 tctagatggc tggaaatact ttggttatga ttacagtgaa gaagaaatca aagaggtaac
    10921 acgggaacgc acccttgaat tcttggaatt acttgaagga aaaccatttg aagaaccaaa
    10981 tcctcaacca atgttcccac aaggatcagg aaaattacgg atagaacctt attcagaagg
    11041 attacgacaa agaatctggt ggggagctgg ttcgcagcaa acggcaatct gggcagcaca
    11101 acatggaatg aatctgcaat catctacatt agtcaattac gaatcccacg agccattcca
    11161 tatccaacaa gcaaaccaat tacgtgcgta taaagaagcg tggaagcaag caggtcatga
    11221 ttttgaacca agaacattag ttactcgctc gattcagcca atcaccactg atttggatcg
    11281 acgcttattt ggacaagacg gtagtccgca acaagatgct gtcggctatc ttgcttatca
    11341 ccgaaaccca acgatttttg gaaaaacttt tgctggtgaa cctgatcaat taatcaagga
    11401 gctagctgaa gatgaagcaa tcaaagaagc agacacagta cttgtcacga ttcctaatac
    11461 tttgggggta gaatataaca tgcacttgct tgaaacaatc gtaaaagata tcgctccaga
    11521 attaggatgg cggtaaaaag gatatttttg taagtgcagg atacttgagt gaaaaaataa
    11581 acacttctaa caatcagtta gaggtgttta ttcatgtttt catgaagttc atagagaagg
    11641 agagtgagtt atccgtttgt tcaatgagaa tgtacataag cttcttttac atgttggggt
    11701 aaatcatcag gaatcattgt caatacggct tcttcattgc cggcttttat tgcttcttca
    11761 tagtaccagc ttttgtagtt tagcatagcg agtactcttt ctaattttgc tatttctttt
    11821 tctactactt ctttttgttt ttcaaatagt tctttacgaa cctcaaatgt cttactgcct
    11881 tctttgctcc attccatgaa ttgcttaatg tcttttattt ctaaaccaga ttttttcaaa
    11941 cagtcaatga ctcttaaagc ctctaactca ttctctgaaa attttctaat accagaaaca
    12001 cgctggatat caggaaataa tccctctttg tcgtaataac gtaaagtaga aacaggcaat
    12061 ccaaacattt cagacacttg tccaatggaa tacataaaaa aatcctccta aaataaaatt
    12121 tttaccttg
//
LOCUS       DASOUD010000077        11839 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.077, whole genome shotgun sequence.
ACCESSION   DASOUD010000077 DASOUD010000000
VERSION     DASOUD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.077"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(113..694)
                     /locus_tag="U4A29_001993"
     CDS             complement(113..694)
                     /locus_tag="U4A29_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO0681112.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(877..1503)
                     /locus_tag="U4A29_001994"
     CDS             complement(877..1503)
                     /locus_tag="U4A29_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO0681113.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1525..2856)
                     /locus_tag="U4A29_001995"
     CDS             complement(1525..2856)
                     /locus_tag="U4A29_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO0681114.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3011..3412)
                     /locus_tag="U4A29_001996"
     CDS             complement(3011..3412)
                     /locus_tag="U4A29_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEO0681115.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3498..3704)
                     /locus_tag="U4A29_001997"
     CDS             complement(3498..3704)
                     /locus_tag="U4A29_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681116.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3799..5301)
                     /locus_tag="U4A29_001998"
     CDS             complement(3799..5301)
                     /locus_tag="U4A29_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEO0681117.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5319..5774)
                     /gene="argR"
                     /locus_tag="U4A29_001999"
     CDS             complement(5319..5774)
                     /gene="argR"
                     /locus_tag="U4A29_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO0681118.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5872..6489)
                     /locus_tag="U4A29_002000"
     CDS             complement(5872..6489)
                     /locus_tag="U4A29_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEO0681119.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6710..8083
                     /locus_tag="U4A29_002001"
     CDS             6710..8083
                     /locus_tag="U4A29_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO0681120.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8158..9270
                     /locus_tag="U4A29_002002"
     CDS             8158..9270
                     /locus_tag="U4A29_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO0681121.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9342..10979)
                     /locus_tag="U4A29_002003"
     CDS             complement(9342..10979)
                     /locus_tag="U4A29_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEO0681122.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11147..11755)
                     /locus_tag="U4A29_002004"
     CDS             complement(11147..11755)
                     /locus_tag="U4A29_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO0681123.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3122 a         2493 c         2142 g         4082 t
ORIGIN      
        1 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
       61 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa
      121 ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata
      181 gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg
      241 cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt
      301 tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca
      361 ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct
      421 catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag
      481 catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg
      541 ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag
      601 aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt
      661 tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat
      721 agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt
      781 gccaaattat ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt
      841 gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc
      901 ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc
      961 ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctac
     1021 caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt
     1081 gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt
     1141 atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc
     1201 ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc
     1261 aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata
     1321 atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat
     1381 attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc
     1441 tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa
     1501 catggtggat tctccattcg ttatttattt taatacatca tttagagctt cactcattgt
     1561 cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag
     1621 cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa
     1681 gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc
     1741 tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc
     1801 ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac
     1861 agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg
     1921 ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca
     1981 aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag
     2041 tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc
     2101 caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc
     2161 tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc
     2221 atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc
     2281 agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc
     2341 caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat
     2401 cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat
     2461 tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt
     2521 attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc
     2581 agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc
     2641 tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt
     2701 agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac
     2761 gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc
     2821 aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct
     2881 accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta
     2941 tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc
     3001 ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt
     3061 aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat
     3121 cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag
     3181 agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta
     3241 ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt
     3301 ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt
     3361 ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc
     3421 tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct
     3481 ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa
     3541 aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc
     3601 ttttctacat aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc
     3661 ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc
     3721 tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag
     3781 cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa
     3841 taattttgct aattttacta acgccaactt gttctttccg aattcatcat tttctatctg
     3901 taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg
     3961 ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc
     4021 ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc
     4081 gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg
     4141 ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat
     4201 gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc
     4261 gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt
     4321 tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag
     4381 atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat
     4441 tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag cactgacaac
     4501 tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac
     4561 agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa
     4621 tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac
     4681 tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt
     4741 tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta aatcttgcgc
     4801 ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg
     4861 ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa
     4921 ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca
     4981 cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc
     5041 gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc
     5101 gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt
     5161 cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt
     5221 caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg
     5281 cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc
     5341 ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca
     5401 ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca
     5461 tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa
     5521 acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta
     5581 aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga
     5641 atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc
     5701 agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc
     5761 tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt
     5821 tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt
     5881 ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct
     5941 gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc
     6001 ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta agatttgggc
     6061 acaaagacct tcttccatct ttttgaatac tagttgttct ttcaagtctt ccggaagttt
     6121 gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat
     6181 catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg
     6241 cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca
     6301 atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg
     6361 tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg gttttttagg
     6421 aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat
     6481 tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt
     6541 tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt
     6601 aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta
     6661 taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca
     6721 aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt
     6781 attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac
     6841 gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc
     6901 gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct
     6961 gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt
     7021 tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat
     7081 cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac
     7141 ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg
     7201 gaatcatatc ttcctgtttc ttgtgcccgt tttgattctt tctttgatta tcggctggtt
     7261 tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg
     7321 aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag
     7381 ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa
     7441 agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt
     7501 gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt
     7561 ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc
     7621 agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa
     7681 gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact
     7741 cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat
     7801 cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca
     7861 aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc
     7921 attagtagcg gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc
     7981 tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt
     8041 tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg
     8101 tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg
     8161 gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat
     8221 acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc
     8281 gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct
     8341 aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt
     8401 tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt
     8461 cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct
     8521 gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt
     8581 ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa
     8641 caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc
     8701 aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt
     8761 aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc
     8821 ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga
     8881 gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac
     8941 gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac taagagtaca
     9001 gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt
     9061 acccgtgctt atacgtcaga tttttcccca tttttcggcg tactgccaga tgatccaagc
     9121 ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat
     9181 ctattagcca acttcttaaa tactggtcat tggtctacgg aagattatca aaaggacatc
     9241 tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga
     9301 gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc
     9361 aaattctcca aactttcttt tgcttcatct ggcaagagat cttttcctat ctgagcttca
     9421 atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca
     9481 aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac
     9541 ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg
     9601 atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa
     9661 aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca
     9721 aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat
     9781 cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata
     9841 attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga
     9901 tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt
     9961 atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga
    10021 caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag
    10081 taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca
    10141 tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca
    10201 gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc atattgtttc
    10261 atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa
    10321 gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga
    10381 gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag
    10441 atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa
    10501 ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc
    10561 agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa
    10621 aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt
    10681 tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca
    10741 tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt
    10801 gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttcctg
    10861 tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg
    10921 gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg
    10981 gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt
    11041 atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa
    11101 cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt
    11161 ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt
    11221 ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt
    11281 gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg
    11341 cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag
    11401 ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg
    11461 tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg
    11521 ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag
    11581 caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat
    11641 cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg
    11701 cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa
    11761 actccttttt ttctccgttt tttattcttt cgacgctatt aactttatca tttagaaata
    11821 tttcataggt aaaagaaac
//
LOCUS       DASOUD010000078        11592 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.078, whole genome shotgun sequence.
ACCESSION   DASOUD010000078 DASOUD010000000
VERSION     DASOUD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.078"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..971
                     /locus_tag="U4A29_002005"
     CDS             108..971
                     /locus_tag="U4A29_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO0681124.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1100..2722)
                     /locus_tag="U4A29_002006"
     CDS             complement(1100..2722)
                     /locus_tag="U4A29_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0681125.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2971..3492
                     /locus_tag="U4A29_002007"
     CDS             2971..3492
                     /locus_tag="U4A29_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO0681126.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="U4A29_002008"
     CDS             complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="U4A29_002008"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEO0681127.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5573..6391
                     /locus_tag="U4A29_002009"
     CDS             5573..6391
                     /locus_tag="U4A29_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEO0681128.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6494..6691)
                     /locus_tag="U4A29_002010"
     CDS             complement(6494..6691)
                     /locus_tag="U4A29_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681129.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6860..7438
                     /locus_tag="U4A29_002011"
     CDS             6860..7438
                     /locus_tag="U4A29_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO0681130.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7533..7670
                     /locus_tag="U4A29_002012"
     CDS             7533..7670
                     /locus_tag="U4A29_002012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681131.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8483..8986)
                     /locus_tag="U4A29_002013"
     CDS             complement(8483..8986)
                     /locus_tag="U4A29_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO0681132.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9345..10370
                     /locus_tag="U4A29_002014"
     CDS             9345..10370
                     /locus_tag="U4A29_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEO0681133.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10693..11571
                     /locus_tag="U4A29_002015"
     CDS             10693..11571
                     /locus_tag="U4A29_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEO0681134.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3590 a         1973 c         2257 g         3772 t
ORIGIN      
        1 attttgatta gaacttgcga aaataaaccg gttaaagtgt gtctagcgca agagatttgc
       61 tataatagac gataagggta aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg
      121 taacagattc ttgttgtgat ctgccatacc atgtattagc aaaagaaaaa gttgattttt
      181 tatcaatgaa tctccaattg aatgatcaag agttagtaga tgacctcgga aagacattca
      241 attatgaccg ctacatgaat cagctgaaaa atggtgcgat gcctacgaca tcacaaatca
      301 atgttggccg atatttagaa tttttccgtt cttatgcgga aaaacagatt cctgttttat
      361 atgtggcttt ttccagtgga ttaagcggct cttacagtag tgcgcttcaa gcggttgaat
      421 tgctgaagga agaatatgaa gaccctgaga tctatgtttt tgatacaaaa gctgcaagta
      481 ttggggaagg gcttttagta ttggaagctg taagattgaa aaaagaaggt aaaagcatcg
      541 aagaaaccct agagtggctg gaaagtaatt atttgaaagt ccattcatgg gtaacagtag
      601 acgatctgaa atatctagaa cgaggcggta ggatttccaa agcaagtgct actcttggca
      661 gtctattgag tgtaaagcct attattacgg tggataaaga aggaaggctg acgaatgttg
      721 acaaagttcg aggaagaaac aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa
      781 tcgttgcacc acttgatcaa atcgtttatg tggcttacgc tggggatcta gagagcgcgg
      841 agaaggcaaa acggcttttg gaagagcaga taaagatgaa agatgtgaag ctttatccct
      901 taggaccaac gatcgccagt cacacaggct atggctgtat cgcgattttt tcgatgggaa
      961 tcagtcgata aaagcagtaa ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa
     1021 acagcgactc ttaaaagata gatgagcatc ctatctctta gaggtcgctg tttttttatt
     1081 ttttaagcta aataggccat tatgcccata actcgttcaa ttgtttttga atatctgcat
     1141 tttccaaaaa ttcatcatat gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg
     1201 caatgatgcg gtttgccaat gtttggataa attgatggtc atgtgacgca aataagattg
     1261 agccagtaaa tgccatcaag ccgtcattca atgctgtgat tgattctaag tctaaatggt
     1321 tcgttggatc atctaataca agtacgttag ctttcgaaag catcattttt gaaagcatgc
     1381 aacggacttt ctctccacca gataaaacat tcactggttt caatacttct tcaccagaaa
     1441 acaacattct tcctaagaag cttcttaaga aggtgttatc atcttcttct ttacttgcat
     1501 attgacgcaa ccagtctaaa atcgttagat cagtatcgaa ttctttcgtt gtatcctttg
     1561 gtaaataaga attacttgtt gtcacacccc aacgtactgt tccagtatct ggcgtaattt
     1621 cacccatgat aactttgaat agcgttgttg ttgttatatc attttctgcg acgaaagcga
     1681 ccttgtcttc tttgctcaaa ttaaaagata tatcatctaa aattttcttt ccatcaatcg
     1741 ttacacttac attttctacc tgcaataagt cgtttccaat ctcacgttca ggtttgaatc
     1801 caacgaatgg ataacgtcta gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca
     1861 tttttttacg tgaagttgct tgttttgatt ttgatgcatt cgcactaaag cgggcaataa
     1921 attcttggag ttctttgatt tgttcttctt ttttggcatt tgcgtttgct tgaagtttag
     1981 ccgctagttg actagattcc aaccaaaaat cataatttcc tacataaagt ttgattttac
     2041 caaaatctag atccgccata tgtgtacata ctttgttcaa aaagtgacgg tcatgggata
     2101 caacgatcac agtattttca aaattgatca aaaattcttc cagccaattg atcgattgga
     2161 tatccagacc gtttgttggt tcgtctagca aaagaacatc aggtttacca aatagtgttt
     2221 gtgctaataa cacttttacc ttttgtccac cagttaattc actcatttgc tgatggtgca
     2281 attcttcagg gatgttcaga ccttgcagta aaacagctgc ttctggttca gcttcccatc
     2341 cattaagctc agcaaattct ccttctaatt cagctgctcg aataccatct tcgtcagtga
     2401 aatcctcttt catgtaaatc gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc
     2461 ccatgatgac tgtttcaagt acagtatagt cttcatagtc gtaatggttc tgtttcaata
     2521 cggccatccg ttcatctggt cccatagtaa cggaacctgt ggaaggctgc agatcccctg
     2581 ataacacttt aaggaacgtt gatttacctg caccatttgc accaatcaag ccatagcagt
     2641 ttccgggggt aaatttaata ttcacatcat caaatagttt gcggtctgaa aagtgcaaac
     2701 tcacatcatt tacagtaatc aatttttttc ctcactttta tcattattag ttttgacaag
     2761 tcttatagaa ttatataggg gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt
     2821 ttcacgtgaa acagaatgaa aaaaataaaa ataaaagcgg agcgaaaata gctactatag
     2881 gatttattag tttgcaacag cataattttt gacgttattt ataagaggga gtactataaa
     2941 actgtaaaag aaatgaaaga aggattgaaa atggatgaaa aaagaaccat cgactggggc
     3001 tcattgattt tgggaatttt atttgtactt gtctcattgt tatcatttca agaccctgtc
     3061 ggaaatttag ttgcaattgt agtagtcttt gcgatttttg cctttattaa agggatattt
     3121 gaactgtttg tacggaacag attaaaagag ctaacaggat ataaaggaaa gatgccctta
     3181 ataatcggga tcatcgatat tctagttggc gtctttttct tattcaatat tggtgcagga
     3241 gtcgcagcat tgccttttgt ttttgcagta tggtttattg ctgattcagt tttagcgtta
     3301 tttactgctg acttggcaag aggagtaagt gaaggatatt attggtttac gatcatcgtc
     3361 aatattttag gaattttatt aggaattttc ttattgttta atccaatttc ttctgcgttg
     3421 acattaagct ttttagttgg attctatttt atgttatttg gtatcacaca tatcgtctat
     3481 gcatttagat aagtaaaatc aaacaaataa aaaagccggc ttccgattac agttttactg
     3541 ttgtaattgg aagccggctt ttttatcatg gacaaatatg cacgaggtga tttttaggtg
     3601 tcgagacaaa tttatgcaaa aatgtgagaa gctatataca caaattttat cttaatactc
     3661 gaagcatagc gcagttgtca caacctcttt tcatataatt cattcaatac tggcaaatca
     3721 tggacttcat atttttcttt gaatgcttgg atttcttctt cagaatattt tcttggatct
     3781 aaatgcagat cttctactgg caatgggcgc ttgttattga tcttcacatc gattactaca
     3841 ggacgatctg attttgcagc tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact
     3901 gtaaagctat ttgccccgag cgcttctcca actttaccga aatctgcatc ttctaaaaat
     3961 acaccaaatt tcttttgttc cgtatcttct tgttctgctt cgatgaaacc aaaagaatca
     4021 ttcgaaaaga caacattaat aattggaagc tgatatttga cttgtgtaat gatatcttgc
     4081 atgaccatag caaacccgcc atcaccactt aaggtaaata cttgtttttc tgggtagctt
     4141 agttgtgcgg caattcctcc tggcacacca ttacccatag ttgcgaacca tccagatgtc
     4201 gtatgttttt gtttaccgtt catattcaat aaacgaatag aatggatagt cgtgttaccg
     4261 acatctgtga cgaaaatcgc atcgtcttca gcaatacgat tgatttcttt atagacagcc
     4321 tctggacgaa tcggttcttc ctctcgatct tcaaaagatt tcaaccaatt tacccatttt
     4381 tctttatttt cttgatttgc taggtaccag ctatcagcag ggcgagcatc ccctaactcg
     4441 acaagtcttt gcaaagctgt gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt
     4501 cgtccaaatt ttgacgcatc gatatcgatt tggataaatt cagcagttgg attaaaaaat
     4561 gcacgtccaa atgggaagtc gctaccgaca aataagatca gatcggcttc tgccaatgct
     4621 tcatttccgg gtttagtagc gacacgtcca gcaaaaccaa gaaagttttc ataatcatca
     4681 ggaataattc cttttgctag aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat
     4741 tctttgattt gcggaaatcc gtttcgcgtt ccttgaccaa cataaagaac tggttttttt
     4801 gctttttcaa gataaggcaa agcagctagc aaatcttttt cttctggtaa tatcacactt
     4861 gttttgtgag tgtgagcggt ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca
     4921 acagggatag tcacgatagc cacgccttta tgctcatagg cggctttaat ggcttcatct
     4981 actacatgcg gaagactttg tggtgtcatg actgtacgat tgtatacgct aacatctgcg
     5041 aaaataggat tttcatttag ttcctggaaa gagttatagt tcatggatgt agaagcaact
     5101 tgtcctaaga acgctactag aggcacatgg tccatctgtg catcatacaa accattgatc
     5161 aaatgtgtcg caccaggtcc tgcagagccg aagacagcgc cgactttccc cgttaatttt
     5221 gcatctgcag cggctgcgag tgcgccgact tcttcatgac gaacttgaat gtagttgatt
     5281 tcggtttttt cttgatatag cgcatccata attgaattga aagatcctcc gggaatgccg
     5341 taaatatggt cgatacccca gctttcaaga acttttacca ttgcgattcc tgcattgatt
     5401 tttgacatgg tgtttcactc ctttagcaat gatgatacat aaattttttg tatctagagt
     5461 atagaacttt ttatcgaaaa tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt
     5521 tttgggaata tgctagagta gtacatagat agattcaaga gaggggaatg gcatggttga
     5581 atggctattg agacttgaac cttggcagca agcacttgct gggacagctt ttacttattt
     5641 tatgacagct cttggtgccg ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct
     5701 aaacttgatg ttaggatttg cttctggtgt catgattgct gcaagttttt ggtctttgtt
     5761 agatccggct attacaaaag cagaagaaaa tggagatatt gcttggctag ttgtaagtat
     5821 tgggtttggt ttaggcggtg tgtttttata tatggcagat aaaacactgc cccatatgca
     5881 ttttggacca caacatgaag cagaaggatt gcctacccat ttaaaacgga cgatcttgct
     5941 tgttttttct attacattgc ataacattcc tgaaggttta gccgtggggg tagcattcgg
     6001 tgcagcagca actgcggata atccgacagc cgctatatta gccgctgttt cggtggcgct
     6061 aggtattggt atccaaaact tcccagaagg agcagctgta tcaatcccgt tgcgacaaga
     6121 aggtctgagt cgtaaaaagg cctttgttta cggacaagct tctggaatcg ttgaaccaat
     6181 tgctggagtg attggagcgc tactagtaac gaaagtggag ctgctgcttc cttatgcact
     6241 tgcttttgcc gcaggagcaa tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca
     6301 gcagacattg tccagcaaac gtcattttgc agtttttggc gtcatgagtg gatttatcat
     6361 tatgatgatt ttggatgtgg cattaggata aataaaaaaa atatatatga tatatcaaat
     6421 agggccgcga acttatatcg cgaccctatt tttttagtaa gttatatcat aatgaagtga
     6481 atggtatcat acattaataa tagttttttc tatgtgtatt tcctttgatc cagcgaacaa
     6541 gccagatgat aagtgcgatt gggattagaa ctttgaccgc aaaccataaa agcccgctta
     6601 ggacactgac aaaaagactg atgacgaagc tagctgcaat tagcactaaa agaaccatga
     6661 agatatttaa gaacatattt ttttgacgca tacaaaaccc tcctcttgtt cattctttac
     6721 tatagaaaaa gtataacagg gattgtttaa aaacacctcc aactgaagac tgatttttac
     6781 ttcatctact taaatgatca aacaattgct ttttctctgg tttatcatct aaaatagagc
     6841 ggaaaaggag gagaatatca tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat
     6901 cgcgattatc atgactattt tagttttaga acttcatgaa cctaacggag acacgtggga
     6961 ggcgctcgct gcattaggtt accgattttt tatctatatt gtcagtttta tcattttagc
     7021 gatttattgg aacaatcatc atcatttatt tcaagttgta cataagataa atggacgggt
     7081 cttatgggcg aacagtttct ttgtttttgc gctttcgctt tttccgtttg caacttcatg
     7141 gatcagtgag tacatcaata gcctggctcc agaaatgact tatggttttg ttaccttgac
     7201 cgctaatatt gcgtattata tcttggcaaa agaacttgtc aaagcaaata aaggccacga
     7261 gaaagcattg aaggcgatgt tgaataatta caagaaatcg tatctatcga ttggtttgaa
     7321 tgtgttagga attgtcttag ggtatttagt agcgccagta gccgtgctga tcgcaaacat
     7381 cgtgattcta gctgcttgga tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa
     7441 tgatccgtgt ttaaaattta agagaaaaat atagaaaaaa taaataaacg atcaaatatc
     7501 cgagtgatac tagtattcaa agagggatgc agatggatca aaaaagaatt catgtaggac
     7561 tgattactag ttttgtcacg atgcttattt atttggatca aggtcatttg acttggacag
     7621 tagttggtgt cgtaggattt ttgacattat tcacagcaat aaaaaaataa acatctttat
     7681 gtaatctgat gttctatcta tttaagaaaa gcatgtttac agcagtatga ttctgttgta
     7741 aacgtgtttt tatttgtgga aaatttaatt tttagaaaag tgtatcgaaa aatagatagt
     7801 tagtttttcc acagtggata acttcgtaag agataaaaat aaaaaattag tcaaagaagg
     7861 gcgtatagat ttcttttgat aggagatata caacagaaat gaataaatat taacgtgttt
     7921 tcaaacagta taaaacggtg tggtttttgg aataaaaaaa taacatatct cattttacac
     7981 ttttgttatt ttatacttaa aaaataacga cacagaaaat accgtgtctc agatttctct
     8041 actacttcta aaaactactg tttttttcct ttgaaatgcc ttcaaaaagt aggatcaggc
     8101 agaatatact ggcagaatca aggagaaggg agcctaaata aggagaaaaa ccatagaaaa
     8161 tataaagtaa ttacttataa aacaagcttt ttatagaaaa ataatatacc tgagtagggt
     8221 ataaattaag acggaattcc tttctagatt gagatattga ctaggaagaa tgttaagatt
     8281 ttaagccttt aagctaaaaa aaaacccttt ttgttaaata aggggaatat agcctgtcag
     8341 tttcaaactg caagtcgtcc tgctgcaata atatagtaga ttagagatga aaatagattt
     8401 ttttaaacca atgactgaat aaaaagaccg tcatagatac acattggaat gtgtctatta
     8461 cggtcttttt ttacgaacga ttttattgaa gacggacagt tcgggtcaaa aataaaaaag
     8521 ctacgatcag aaataagaat aaaatgtatc ccaaaataat ggctagacgg tctaacttat
     8581 ttgtcgatag atgtttgttt agaagttgat aggcccataa acctatcagt cctcctgttg
     8641 tattagttat caaatcagtg atgtccgcta cgccaatacc gaataggaat tgaatcaatt
     8701 ctgccaagaa gcttacaagg aaaataatcc ctaatttttt agaaaaagaa agtctcttga
     8761 aattgacatt caaaagcacc ccaaaaggaa aaaagacaat caagttataa gtggtctcac
     8821 gtaataccgt cgtattttca aaagaaaaag tagaaaaagg gacgagattg acattgctgt
     8881 aagaataagt gagaaattgt ggaagattaa atgatagctt gaataaaatc aaccataata
     8941 gaaagaataa ataaatccct aataaaaatt ttgatacata ccgcatagac atctcctcca
     9001 tttaaaaagt ataccatcga aaaccgctga aaaatcataa aaaagtatga agtttaagga
     9061 ccaacgtagt tatcgtacga aaattagaag cttttttttg ttgggaatcc ggtttcttta
     9121 acaaagctaa gatagcgagt tgcttggaaa agttgctatc ttttttctgt caaaacgact
     9181 cggaatgttc gttttttgtt ctaatttctc caagttgttt actttttgat actaagttta
     9241 tgaaaaatca agaaatagtt gttatactag gtaatgctga caaacgggat tggcatttca
     9301 tcttggttca gctttttttc caattactag gaaggaggga tagattgttc aagacttatt
     9361 ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat gaatctgatg ctacttatct
     9421 ttattgtatt atggttaggt tacataaatc atgatttttt gatttcaagt ttttcatcat
     9481 tagggatctt tacttttttc tattatcaaa acataccgat gaaatatttg ctgaaacgta
     9541 tgtttctgat cggttgcgga ctgttggttg ctttcgtttt ggggatctta tcgacttatg
     9601 ttttatggct tgaaccattt gccgtagctt tggttgcttt ttccagcaga ttcgtgttgc
     9661 gattattcca tatttctaaa ccaggtggct tgttttttgc gatgctttct gccatgggaa
     9721 cgagcatgca gctgccaatc gctcaattac cgatcgtctc actttatttt tttatgggtg
     9781 tcgtttttgc cctgattgcg gcagtgatta caaaattact ggattcacgg cctgagcaga
     9841 caattgaaaa agcaacttta aaagaaagat ttcacgaaga gcctttagtg atcattgatt
     9901 ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt cagtcacggc ttgaatctgc
     9961 ataatcctta ttggttgacc ttgtcatgtg catcgatttt gctagcagaa aacttggatg
    10021 cgatgaagca tcgacaagtc caatatctga ttggatcgat gggtgggtta tgtgtgtcgg
    10081 cattcttgtc atttgtgccg tttacgcagt tgcagaccat ttttctgatc acttttctat
    10141 atggaatcgc acaatttcta gttgctagaa attatgcagt tgctaatatt tttcttaatc
    10201 cgatggcgtt gatgctatct acacttattc gtggagcata cttgatttct ttgatcgaat
    10261 atcgtttttt agggatcgtc ataggaagtt tcatcggttt aggtgttgcg tgggtaatga
    10321 cggttggttt gcaacattat ttaacggttg ttcgtcaaaa attagaatag aaggacaaat
    10381 atatgtgcca aaagaggtta tgacaagatt tttgtcacaa cctctaattc aaggtgttca
    10441 aaagccagat tctcagcatt aagtcaaatt cacacaaaaa tgtggaaagg aactttcaaa
    10501 aaaattttat atttattcta tcaaaaaatt ttgtttatga tggtaactga ataaactttc
    10561 ctgagatggt caatgctttc cttgaaagtc ttacagatat tctttcttca tagaaagttt
    10621 aagttttata gttagtgtac aataaaagta tgtttatacc atatggtgtc cggataatca
    10681 gtcatttaaa caatgaggtt caaagatatg aaaaggggaa atttgatgaa gaaaataagc
    10741 aatgaaatcc aaattctggc agaaacttta actcttccca aagtcgttat agagcaaatg
    10801 attgtacaag caaaaaacat ccttgatgag gtgtttgagc aatatagtca tgaactaatg
    10861 gatcctaata aagcaaaggc gactgcaaag aaaatagaca aagtctatga agaggaacct
    10921 tttgcagcat tagctattta tctatatgca gcttctcaaa gttggaaaaa aatttatcaa
    10981 ccattgaaga ttccaagaac tatctatttg gcaacaatga atgcttttac tagatttatt
    11041 catgaacatt tccaagtaac aggaagctat cgctttgacc gtggtttttg gatttggcat
    11101 tacactagtg gcttgatctt tcggattggt gaattggagt acgaaatgac atattttccg
    11161 aaagggtaca cggatctaag attagaagga aagcagtgcc tttctcttca tattccttcc
    11221 gatgcagatt tatctcagaa taagataaga gatagttacg aaagtgccat tcgttttttt
    11281 gaactttatt ttcctgatta ccattaccaa gtgatgtaca cagatacttg gttactatca
    11341 cccaatctta ctaaatggtt gaaaaaagag tcaaaaatct gtctttttgc agccgattat
    11401 cgattattat cagttgatga acaggacgat agtggagtcc cttggatctt tggacgagta
    11461 gatgcccaaa tacatgatta tccagaaagt acgagtttac agcgacaagc aaaagagcaa
    11521 ttgcttgcag gcgagcacat tggctctggc ttaggtattc ttacttcata agtttagtac
    11581 ttcgaaatag tt
//
LOCUS       DASOUD010000079        11221 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.079, whole genome shotgun sequence.
ACCESSION   DASOUD010000079 DASOUD010000000
VERSION     DASOUD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.079"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="U4A29_002016"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="U4A29_002016"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO0681135.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="U4A29_002017"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="U4A29_002017"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO0681136.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="U4A29_002018"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="U4A29_002018"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO0681137.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="U4A29_002019"
     CDS             complement(3376..4683)
                     /locus_tag="U4A29_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO0681138.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="U4A29_002020"
     CDS             complement(4686..5267)
                     /locus_tag="U4A29_002020"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO0681139.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="U4A29_002021"
     CDS             complement(5839..7131)
                     /locus_tag="U4A29_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO0681140.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="U4A29_002022"
     CDS             complement(7128..7751)
                     /locus_tag="U4A29_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681141.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="U4A29_002023"
     CDS             complement(7754..8590)
                     /locus_tag="U4A29_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO0681142.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="U4A29_002024"
     CDS             complement(8607..9494)
                     /locus_tag="U4A29_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0681143.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="U4A29_002025"
     CDS             complement(9510..10793)
                     /locus_tag="U4A29_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO0681144.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
BASE COUNT         3380 a         2534 c         1882 g         3425 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 a
//
LOCUS       DASOUD010000080        10906 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.080, whole genome shotgun sequence.
ACCESSION   DASOUD010000080 DASOUD010000000
VERSION     DASOUD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.080"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(127..897)
                     /locus_tag="U4A29_002026"
     CDS             complement(127..897)
                     /locus_tag="U4A29_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0681145.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(890..1825)
                     /locus_tag="U4A29_002027"
     CDS             complement(890..1825)
                     /locus_tag="U4A29_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0681146.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2419..4068)
                     /locus_tag="U4A29_002028"
     CDS             complement(2419..4068)
                     /locus_tag="U4A29_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO0681147.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(4263..5159)
                     /locus_tag="U4A29_002029"
     CDS             complement(4263..5159)
                     /locus_tag="U4A29_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO0681148.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(5152..5832)
                     /locus_tag="U4A29_002030"
     CDS             complement(5152..5832)
                     /locus_tag="U4A29_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0681149.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(5833..6615)
                     /locus_tag="U4A29_002031"
     CDS             complement(5833..6615)
                     /locus_tag="U4A29_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0681150.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(6608..7525)
                     /locus_tag="U4A29_002032"
     CDS             complement(6608..7525)
                     /locus_tag="U4A29_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0681151.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(7528..7812)
                     /locus_tag="U4A29_002033"
     CDS             complement(7528..7812)
                     /locus_tag="U4A29_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681152.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(8513..9133)
                     /locus_tag="U4A29_002034"
     CDS             complement(8513..9133)
                     /locus_tag="U4A29_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681153.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            9653..10831
                     /locus_tag="U4A29_002035"
                     /pseudo
     CDS             9653..10831
                     /locus_tag="U4A29_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT         3344 a         2303 c         1840 g         3419 t
ORIGIN      
        1 tccactaaac ggtgttcaat cagctgaccc tcggtattcg ctcaaaaaac aacaaaatat
       61 gaggaactat tttcccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
      121 tcttttctaa cttctcaatc ctcttccttt attgatcaaa taccaagtaa cggcgtacaa
      181 aacaacgatg aataacacaa gtacgatcat cgatgttgct actggcacat cgatcgttcc
      241 aaggaatccg taacggaaac cggagatcat ataaacgatc ggattgactt ttgaaacagc
      301 ttgccaaaag ggcggtaaca tagagatggc atagaaaacg ccacctagat aagttaacgg
      361 ctgcaagaca aatgtaggca cgatcgatac atcatcataa gactgagcaa aaatcccgtt
      421 gagcagtcct gctaatgaaa acaagattgc cgtcatcaat aatgtaatga tgaccatgaa
      481 ccatgaatac acatgaagtg gtacaaagaa caaggaaatg atcgttacga gagagccgac
      541 gagaatactg cgacccacac caccaatcac aaagccccaa ataatcacat gcgtaggaac
      601 aggtgctacc aagatttctt cgatattttt ctgaaatttt tgagagaaga aggacgaaga
      661 aacattggca tacgaactag tgatagcaga catcataatc aaaccaggaa cgatgaattc
      721 catatatgaa aaaccaccca tgtcaccgat ccgcccaccg atcatttttc cgaaaataac
      781 aaaatacaag gatgtcgtga tcactggcgg aaccagtgtt tggacccata tacgcaaata
      841 gcggttcgtt tccttgatag ctaaactttt taatgcagta aaataaagat taaacatttt
      901 tctcctccgt atgattattt tcttctgtga ttttcaagaa cagttcttct aaacgattcg
      961 acttattcct catagaaaga actttgattc cttgtcttgt aagctgatcg aagattccgt
     1021 tcaccccttg atttcgttca acttcgacag cgattgtctg ctcatcttca aatgtacaag
     1081 catatccttc aatcacaggt ttggtatcat atggcgctaa atcaaagata aatgtttcaa
     1141 attgcagttt tgacagaagc tctttcatac ttgtattctc gatcagttca cccgattgga
     1201 tgattccgat gttgcgacaa agcatttcag cttcttctag ataatgcgtc gtcaaaatga
     1261 tcgttgtacc attttcgttc aattctcgta gaaactccca catctcgcgt ctcaactcga
     1321 tgtcaactcc tgctgttggt tcatccaaga tcagcaaacg tggttcatgc atcaatgccc
     1381 tagcaatcat cagtcttcgc ttcattcccc cggaaagcat acgtgcacgg acatttctct
     1441 tttcccaaag attggactgc ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag
     1501 agacaccgta gtagcctgct tgattcacga cgatttgctg gaccgtttca aaagggttaa
     1561 aattgaactc ttgtggaacc aatccaatct gttgctttgc tcgaactaga tccttgtcca
     1621 gatcataacc gaagacattc acttttccag atgttttatt gactaaagaa gtaataatcc
     1681 cgatcgttgt agacttccca gctccatttg gccctaatag cgcatagaaa tctccttctt
     1741 tcaccgaaag atcgattccc cgcaatgctt cgaccccact tccatagatc tttttcaaat
     1801 tatttatttc aagcgcattt gccatttatt tcctatctcc ttatagacat gattcttaat
     1861 tgtatcatgg aatacagcag atgaaaaaac aacttggctt ccacacaaaa acacacctgc
     1921 taacaaagag gagatgtgtt ttctagttca tgatcatctt cgatagaata actatctaaa
     1981 caactctgga acttttaaaa tcataaaagt accgacagca actactacga tgacatatag
     2041 aagatcctta taactgaggt tcttgtcttt tttattcatg ttaagctttc ctccgtcctt
     2101 ttatcgaaag cttttttctt ttattttaac cgaaataaat aaaatggaaa tagttatttt
     2161 caatttctgt tttcgctgtt tgttttacgg agaactttgc catggaacgt gcgtctcgtc
     2221 gctgatgcta agttttttcg ataaaagctg ctagaaggaa gacagcgtgg tttattttgt
     2281 atcgtctttt gctaaatctt ccaagatcat ctggacagca gactcgttcc cattgaattc
     2341 tgccactttt ttggcatcaa tgtacttctt ttgataatcg tctttttgga cccacgcatc
     2401 atgcttcgtt tgataaagtt aatcaaacgg ataccactga cgctgttcat cgaacaacaa
     2461 actttctttt tgggcctgtt caactatgga tttaggataa ttcaaaactt tcagcaaggc
     2521 aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac tgattccttt
     2581 tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta aaatctctgt
     2641 caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa gccaccgaac
     2701 gactgaagcg gaagaagcaa tccgttcgat cgtatttgtt ccttttaaaa tctcatctac
     2761 gaagcagtag cagcgttctt tggaagccac ttggtccagc aaacgtttga tcgatttgat
     2821 ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca tggaagtcaa
     2881 cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg tctgtgctag
     2941 aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat tggaacccgt
     3001 gactaatgta ttcttctgcc agttgactgg attaactacc gcttctttta acaatggatg
     3061 atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag tgatcggcat
     3121 ataggtgcga taattcaaaa cggctgctgc aacttccaat tctcccagca attcccacag
     3181 cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat aggagataaa
     3241 tggcagcatg aacatgatgt tgacatattc aaaaatcaat tctgcttcac tcccgctctt
     3301 cattcgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt gctgtttcag
     3361 ctctttttga agtggtgtcg tcatttttgc taactgattg gcggctgaaa tcgttgaaac
     3421 caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta gataatagac
     3481 cgtattgaat actaagccac cgattgctag atagattcct agtacttgcc ctaaaagaag
     3541 caaacctaca cccaccagcg gaagcaagcc aagaaaaata aataaaccta cgcgtccaat
     3601 ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt tttttcctaa
     3661 acgagcgaat tgatattgga ttctttctct cagttgagga ttttcctgat aaaaagcaat
     3721 tagtttttct aactgataat cttctccaaa atcaaagcta cgtaaacgct gatacaacgc
     3781 ttctgaacct acactagaat aggtactgtt gatcccttca aatacttcga acatatccaa
     3841 gtcataccaa gtaagatcgt cgatttcact atcccatgaa cggaattttt tctccgtgag
     3901 ccacgcatcc ttcaaacttt cttctttatc aaatctcggt tgataaggaa tttttcccca
     3961 attactacga actgtttcct ttactttcaa acgtgaaaaa atgtctaaaa caactaataa
     4021 aataacaact gcaataataa ttaaaacgat aaattctctt tggcccatat ccctctccgt
     4081 tccttgattt gttagtttta gtatagcact gttgcgctat ttgaaaaggg ggtacaaaaa
     4141 taactgtctt cgttacctaa ttcgtatcaa tcacaccaaa ataaaaacac cttccaaaaa
     4201 agaagatgtc tttaaaacga gatacaattt tttgtcattc acttcaggta aattagagcc
     4261 aattaaaccc tccgcaactt gacggagatg gagaagatgc ccccgtctaa tctagcggtc
     4321 atctctcctc ccattccttc caccaatttt tttgacaggt aaagcccgat tccgctagat
     4381 tccgtatttg tgcggctggt attttcggta taaaaacgct ggaagacttt ttcaacagag
     4441 gtttgcgctc ctggtttgat aggattggcc actgttaatc gaatgaatga atcctctttt
     4501 gaaaatgtca cctttgcttg tccatctgca taacgcaata aattctgtat gatattttgc
     4561 agcactcgca gcaacatgag cggatctgct aatacagttg tatttatcga gtgttcagcg
     4621 aacgctacct cgatattttt ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc
     4681 acctctttag aaaaagaaag cgtatggaga tccatttctt tttgcttcgt ctctattcga
     4741 gcaagatcat aaaactgatc cgtcatactg agtaatcttg caacagattg tttgatgatc
     4801 gttaaatact tctcccgttt ctccgtatcc tctacctgtt ccagtaattg tacataaccg
     4861 ttaatagcag tcaaaggagt tctgagatca tgagaaaggc cgacgatcga tagtctgatg
     4921 ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat tttcatccac ccatgtattc
     4981 agtgcatcta ctaggtccat tagattcttt tctcgaaatt cgactgaaag acgaccacca
     5041 tattcttgtc gttcagaaag agtggcgatt ttcttagtta atctcttgat ttccctttgc
     5101 aggaagaaaa taaaacttcc cataaagata acaacgagaa gcaacagcca cttaatcaaa
     5161 tctaaacccc actccccaca ccgtatgaat atacacagca tgtgcctttg ctagtttccc
     5221 tcgaagattg gaaatatgca tattcactgt tttctcacta tcaaaatatg gttcttgcca
     5281 aactgtttca taaagatttg ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt
     5341 cagcagatcg aattctcttc ctgtcaaatg gacagccaca ccattcacat ttacatccct
     5401 cgactctttg tccagtaaga tttccttgta atagatgcgt tgcgacagat cttttacttg
     5461 gacagaagtc tgatgacgaa gttggatgcc gattcgtaac aacagttctt cgatatcaaa
     5521 tggtttcgtc acatagtcat ccgctcctgc tgccaacaag gaaactttat ctctttgatc
     5581 gctttttgct gaaagaacga tgatcggaac attcgatgtc tgtcggattt ctgctaacaa
     5641 tgcttcacct gaaagccctg gaagcatgag atctagaagg atcaaatcga caggatatgt
     5701 gcttaacaat aattttcctt ccgtacctga ataagcatca agaagtttgt atctctgttc
     5761 aagaacctcc tccagtaaag aatgaacagc ttcatcgtct tcgataatca aaatagtttc
     5821 cagcaggaac acctacttaa tgtcagattt ttggaaaata aacattccta gagctgatga
     5881 taccacgatt gttactagac tgaccagaat cattttcggt gcttgcgaat aagttgcttg
     5941 ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac cactccgtta gaaattccaa
     6001 gcctttgatt tttgcgaaaa caccgggaac gaaggccgtt cccatgatat aaagaaaaga
     6061 aagaagcaaa ctgacactgg tacttctagt gatgaatgtc aaagccactg gaacagataa
     6121 tgtcgctaat atcagcagaa gcaatacagg aaatactttt agaacataat ttccatagtt
     6181 tttgattccc tcaacttcac caaatccgaa agcaagtgtg taagcaatca tggctagccc
     6241 cgtaatcaaa accacgccaa gtgttgtgat caaaagcgta acgagaagtt tcgacacata
     6301 ataagtcatt cgattccgtc ctaaagataa cgtattgcga atcgtaccgt tttgaaattc
     6361 ttccccaata taaaagcacg caaatgctgc gaaagccaga agaatccatg cagccatttt
     6421 ttccaccatt aaaatccctg ttgcaccgtt gatgactgta tccgttgctt ggtttaattc
     6481 attgccgtat tttgtatgca tataccattc gttccaagaa taaaaaagcg gaagtacgac
     6541 catcagtcct agtgagaccc acattttacg gctcttccat acttttattt tttcagcagt
     6601 taatagatta aacatgtgga ttgcctccag ttaattgtaa gaaataatct tccagttttt
     6661 gcccaactag gtggatacca tccacaggaa cttttgcctc taccaatgct tgattgatgg
     6721 ctgctacttg gtcaattcct tcaaaaagct ggataacacg gctagattgg acaaaataat
     6781 ccctataacc aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt
     6841 ttataaattg acgagattct tgtgccaatt cttctttcga taattctttg atcaagtgtc
     6901 cttcatgcaa aataccgtag tgagtggcga tctgtgacaa ttcatctagc aagtggctag
     6961 aaagcaagac ggtgatgccc cgttctttta ccaaacgttg gatgatttct cgcatttctg
     7021 ccatccctgc tggatccatc ccattcgtcg gttcatccaa aatcaaaaat tctggattcg
     7081 tgatcagtgc atttgcaata gctaaacgct ggcgcatacc taaagagaaa ttctttgctt
     7141 tctttttccc tgtattttcc aatcgcatca ttttcagcag gtcttcgatt tctctatccg
     7201 acacaccgcc gttcgctgct tggatacgta ggttatctct tgcagttaat tctggataaa
     7261 gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc
     7321 caaataattc gatcgaacct tcatcaatcg agatcaaacc cataatagtt ctcataaagg
     7381 tagacttacc cgctccattc tccccaatca aaccatagat cattccacgt ttgatttcga
     7441 tcgatacttt atctaatgct ttggcagcac cgtatttttt ggttatacca gttgctttca
     7501 agaccgtttc atttccaaaa tccatactta tccttctttc ttattgataa gtgaagtata
     7561 gttgacgggt ataaagaaag cagccagaaa tgtataaaga aagtgtaaat tttccaattg
     7621 aatttcaaga aaaatcagac tgtctcgcag acgtggttga gttgtctgcc aatcaaagag
     7681 cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg ttatcgttga
     7741 catcagacgt ccttgtatat gcgttggaac ttctcgctga agccagacca tataaaaagc
     7801 aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc cgtttgggat
     7861 ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc ccttcttccg
     7921 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta tcaaacatga
     7981 gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     8041 agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt gtttatagat
     8101 cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca caaaaaaggc
     8161 actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc gctcagcatt
     8221 gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca attaacgttt
     8281 tgagaactgt gaagctttac gggctttctt aagacctggt taaacaaaac tctattttat
     8341 tgcattttac aggaatgtat ttcatgcgaa attaataagt ctttgtttat agagttttac
     8401 tagggtataa aaagttgtat gttatcatta cctcaaaaca ttacctcagc ttggcgagtt
     8461 ggttgattga agaataaatc atttatctag atatctcaat aaaaaatgat ttctaagaaa
     8521 tttgtatttt cgtgttatta tttttttgcc ctatcaaaat ttctaattct tctagcgtaa
     8581 tatgattatg acacataatc gattcaatta cttcaatacc tatttttttc gagaaagatc
     8641 ccttttgttc actcacaaaa atactttcag aagacatata taaggaacga agttttggaa
     8701 ttagtatatc gtacaaatag accaccataa aatcgtcctt gcaaccattc tcaaaaagaa
     8761 ttaaatttgc tgattgtgtt acatatatca ctgaattaca ttcatggtca attccaaaaa
     8821 caatgtctac ttcattttct tttctaattc gcgcagttac atttttcaaa ctttcaccat
     8881 tacttataaa tgctttagtt actgtaagct ctgatgtttg ttcagatatt ccttttataa
     8941 tattttgttc atcagccaca ttccccataa atccgccgat tccttttaag taataattct
     9001 cacctaagag tcgcttgcat tcactgaatt gataaaaaaa ccagttaaaa aaatcttctg
     9061 gaaaaataaa ttctgatcga actttattta tatttttagc tcctattaat tttataactc
     9121 gattaatgtt cacttgctgc gatgaacata taactgaata aattgagttg tttaattcaa
     9181 aaaaacaact ttaacatatg catcattaat tcgttatttt ttaggtagat attgatcatt
     9241 gttatctctc tttttttgct tcccatactc cagcttagaa attgccgtta taaaatttat
     9301 tttaaaggtg actttttccc ctgaagtatc ggtagtagga atacttaata cctcagcttc
     9361 tgaggaggta tcccaattat agttatactt tgcatcatca ccataaagaa caattccatt
     9421 tttttatctt gtgccgttag ctttactggt tcgcttactt ttcttgttaa tatcttggac
     9481 tttacatcat cgatattgct tattttatca agcttaaata atttacaaat taatttcgta
     9541 ctagttttta tatttgacga gagtgtaaaa tattttgtgt aaatagaaaa aaggaagtcc
     9601 cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc atatgaacga
     9661 ttttactaca gaaattctaa agactctagc gaacaaaggc gatttgaatg aattattccg
     9721 tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg ctttcctcga
     9781 ttacgaaaag tacgatcgca ttggttttaa cacgggtaat tctcgtaacg gctcctatga
     9841 ccgtacggtc aagaccgagt acggggaact tcatctccag attccgcgcg accgcaacgg
     9901 cgagttcaag caacagactg ttcctgctta tagacggacg aatgatacct tagaggagac
     9961 cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact tgattgagaa
    10021 aatgtatggg catcactaca cgccccaaac catgtccaac atgactaaag tgctgactga
    10081 agaagtaaat gcatttaaag ttagaactct aaatgataag tatgtcgcta ttttcatgga
    10141 tgctacttat attcctttaa aacggcaaac cgtctccaaa gaagcaatct atattgccat
    10201 tggtatacga gaagacggca ctaaagaagt actaagttat gcgattgctc cgactgaatc
    10261 gacctacgtt tggaatgagt tgttacagga catttactcc agaggggttc atgatgtctt
    10321 actgtttatt accgatggct taaaaggcat gaaagatacg attcaccaga tttatcctaa
    10381 agctaaatac caacattgct gtgttcacgt ttcccgtaat attgctcata aagtacgtgt
    10441 caaagatcga aaagaaatct atgatgattt taaggctgtt tatcaagcta gctcaaagga
    10501 agaggcaaat acctttttag ggagtatgat tgagaagtag caaaaaactt atcctaaagt
    10561 gacgcagtca ctgataaaaa atcaagattt attgactttt tatgagtttc cgcctggtat
    10621 ccgccgaagc atttactcaa ctaatttaat cgaatctttc aagaaacaaa tcaagaaata
    10681 cagccgcaga aaagagcagt ttcaaaatga agaatcacta gagcgtttcc tagtatccat
    10741 ctttgataca tacaatcaaa aattcttaaa tagaagccat aaaggcttcc aacaagtgac
    10801 ggatacatta gcttcaatgt ttactgagta acccattatt ttgcagaagg acgagttatt
    10861 tacacaaaat tattgacgct cccaataaaa aaacaaacaa agtaaa
//
LOCUS       DASOUD010000081        10886 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.081, whole genome shotgun sequence.
ACCESSION   DASOUD010000081 DASOUD010000000
VERSION     DASOUD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.081"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..953)
                     /locus_tag="U4A29_002036"
     CDS             complement(<1..953)
                     /locus_tag="U4A29_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEO0681154.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNN"
     gene            complement(1131..1610)
                     /locus_tag="U4A29_002037"
     CDS             complement(1131..1610)
                     /locus_tag="U4A29_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HEO0681155.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            1777..1926
                     /locus_tag="U4A29_002038"
     CDS             1777..1926
                     /locus_tag="U4A29_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681156.1"
                     /translation="MRKVDEYIKKKSQKSKVWQRTFELEYKKLSDSLKKDTYNPNEQS
                     KADNQ"
     gene            complement(1986..2231)
                     /locus_tag="U4A29_002039"
     CDS             complement(1986..2231)
                     /locus_tag="U4A29_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0681157.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            2336..3109
                     /locus_tag="U4A29_002040"
     CDS             2336..3109
                     /locus_tag="U4A29_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681158.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(3338..3586)
                     /locus_tag="U4A29_002041"
     CDS             complement(3338..3586)
                     /locus_tag="U4A29_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEO0681159.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(3685..4035)
                     /locus_tag="U4A29_002042"
     CDS             complement(3685..4035)
                     /locus_tag="U4A29_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681160.1"
                     /translation="MIIENESEEIGRMADENKVVPLETLVINKEEFEQLFLDFFVQYD
                     RIYDFSSSLDLFELEAEIIKNSQEEDGNIIISVEKFLELFSEIFEIDIDYYQPQEVFR
                     LFRDQIIGISQTTK"
     gene            complement(4076..4570)
                     /locus_tag="U4A29_002043"
     CDS             complement(4076..4570)
                     /locus_tag="U4A29_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681161.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTGKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKNGEELYEYAMILDNPEYIESGKSETINYYYELPSERQEYQIVVRRANGYFVGL
                     PFNL"
     gene            complement(4563..4865)
                     /locus_tag="U4A29_002044"
     CDS             complement(4563..4865)
                     /locus_tag="U4A29_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681162.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            complement(5081..6472)
                     /locus_tag="U4A29_002045"
     CDS             complement(5081..6472)
                     /locus_tag="U4A29_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEO0681163.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            complement(6457..7068)
                     /locus_tag="U4A29_002046"
     CDS             complement(6457..7068)
                     /locus_tag="U4A29_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEO0681164.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(7080..7268)
                     /locus_tag="U4A29_002047"
     CDS             complement(7080..7268)
                     /locus_tag="U4A29_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681165.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            complement(7282..9927)
                     /locus_tag="U4A29_002048"
     CDS             complement(7282..9927)
                     /locus_tag="U4A29_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681166.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     RFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSGTSQPRTVDVRENLQNTTSSQNGNRTVDVREN
                     VQSSASNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQEVKEKKRPRF
                     TYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(9914..10132)
                     /locus_tag="U4A29_002049"
     CDS             complement(9914..10132)
                     /locus_tag="U4A29_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681167.1"
                     /translation="MIENHLVDKIHCLDHNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(10134..>10886)
                     /locus_tag="U4A29_002050"
     CDS             complement(10134..>10886)
                     /locus_tag="U4A29_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO0681168.1"
                     /translation="LQKVKLLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKI
                     THQENSQKNLIVQNLNYGTNQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSY
                     KDSKDIKESHNDDLFESLSTKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSD
                     YRQFDNWYKKFNYAIKSAEKEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAK
                     TDHRIKQKNNYIFTSLKNKALELIKLQKKEGI"
BASE COUNT         3251 a         2118 c         1494 g         4023 t
ORIGIN      
        1 ttattattac ttttctctgt aaaacttgca tgtttaagat cccagtaaat tttatcaggc
       61 ttaattgcat ctaaaattat attatccgat aagaatgttt tatttgaatt tagcttcaaa
      121 tttagatccg ataatacttc agataacaat ctgataatca cttcagcttg atcttttcga
      181 accgaaaaaa ttctgtaatc atctctgtaa cgaagtattt tgaactcttt aatctctgtt
      241 ttttctatct tgttcagtaa cattttatca gcatacccaa gtactatttc agctacaaaa
      301 tccatcaata catttccttg aggtataccg ttcgtttgtc cattttgtaa atatcgtatt
      361 ttagaatcta ttaagtttcc aacgccttga gtatcttttc tatgtgtctt agcccattct
      421 tttgaatgta ctgcccacgg gatagtatgt gtgtacattg agctataaca atcggtaata
      481 tctgaatgaa tgcagtagtc atactccaaa gcataactaa tctgtgcttg ttcaaggttc
      541 tcccaccaat ttaaaatagt ttccgcagta tcgctctttg aagaaataga ttccacagga
      601 agtgatatac attcaattcg cttgtcggct tggaattctt caaacttatt tttgatgtga
      661 ttccaattat ttttctgtgt aatcccgttt actaagtcta catatactaa tggatgtaat
      721 atttgaaggg gtctccaagc ataattacca tctttattag ctaatattcg atgattaaca
      781 tcaactgcct cagagagcga tctcttatgt ctaactagtt cagtaaatgg tttgtttttt
      841 aataaatcca ctgctttctc aatgcctact gataaatcaa tatagttggg caacgatgta
      901 ctaaaaaaac ttgatgtttt taaaaagaat tgctttgctt cttgatgatt catctctaaa
      961 atactttttc tagtcctagt caaattcaaa atctcctaga tcaattaagt tgtaatgtaa
     1021 gaggataccc taaggttaca caccctattt actattatac cagtattcat tcgtgataca
     1081 taagggtgta acattaagaa ctctatctaa aagactacaa gtagtcacct ttaattgtga
     1141 gtaattttct ttatccaatc cttgtgcaac cttgtcgtca ccccttgtat atccccgtac
     1201 cccggatgtt tcggaatatc aaaaatgtgc tcaaattcct gattttcatc agtctcccac
     1261 tcatgattca acggttcatg ccactctata aaccttgacc ataaaatgtt ctttctataa
     1321 aactcaatag gaatttcaaa acagtaaaaa ttgtatttgc taaaatctgt aaaattatct
     1381 ttacgagcat actttaaatg gcaggtatct cgccacaacc aaattaaatc tcgatccaat
     1441 ggtaactgaa ttcgtttcgt atactcttct tcaagccaat gaagtgcacc aactgcattc
     1501 ggattatcga aaaagacaga taattcagta tagcgtttct ttggataaat aaatccttct
     1561 gaatcaacag attgcaaaaa atctactggt tgaaccgtgt acactttcat ttattcgcca
     1621 cctttttttg actacttata gtcatttacc aatttgaaat ctgcatactt tttcattcaa
     1681 ttgagcccag cattaattgt acacgagttt tcttcactaa cggatagtat caggatatac
     1741 taatactatc ctgaatagcg aaatgaggta gccaaaatga gaaaagtcga tgaatatatc
     1801 aaaaagaaat cccaaaaaag taaagtatgg caaagaacat ttgagttaga gtataagaaa
     1861 ctctcagata gcctcaaaaa ggacacttat aatccaaatg aacaaagtaa agcagataac
     1921 caataaagat taccctccca tatttaacat tgcttcacga atcagtaatc aatgactaca
     1981 agtagtcatt gaagcaaatc ttcataagat acctgtagaa tatccctaat agctctcaat
     2041 tgatccagct taatatgttg ccggccacct tcaatcttaa ccaatgtttc tcttgtcata
     2101 gcgattttac tgagctggag ttctcgcacc agttctgtct gtcccattcc cttttctaat
     2161 cggattcgtc tgatattgct accgattaca ttttctttat ctattaactt ttcttccttc
     2221 aaaaatagca cgccttttgg actaaaatta gtcctttttc ttattaatct taaagatttg
     2281 gtatgctaca atgggactag tattagtcca tattcaattt taaggaggaa tttttttgaa
     2341 gaaatttgca atcattctaa cctgtgcttt actcacggga gctataagtg ggtgcgccac
     2401 ttctgacaca aattctacaa gtactgaaac aagtacaaca acctcagaaa agaattatcc
     2461 tatacatttt tttccagttc cagatggatt tgaagcttat tatcatgata aaaaactaga
     2521 tgttccattg tcagaaagta actctattga aaaagttttg ccaattgaaa ttgaaaaaaa
     2581 tcaagagaat ataaaactca aggccgagct tcccatactg aacagcacag aaattgattg
     2641 gcacgagaat attaaagtaa ctattgaaaa aggagcgtta gcacaacttt atactgaaaa
     2701 agttgaacaa ttttttgagt ctggtgccaa taaagacttt tcaaaaataa gcgattattc
     2761 ggataacttt tttaatgaga cccaagatga aatcacgaac tcaactttta tggctgaaga
     2821 tatgtacaat atgacgttaa aaaaaataaa agcttacgac catccattaa atctgacact
     2881 aaataatgga ttactctctt ttgtattagc aggttacgtt gactatgaat attcaccaga
     2941 ttatttggag acacctcttc aacacgagaa caatgatatt aattttagat ttgtctacga
     3001 gtcaaaaagt aaaacatggt tagttgatca attaagcact ataaacacaa gcttccatga
     3061 attacctaaa cctgaaaatg aagctgatat ggtcattaaa atcttctaaa aaagaagcgt
     3121 tgaaggagaa atattccttt caacgctctt ttttatatgc ttagaaacat aaataaattg
     3181 tatactttct ttttagcccc tattactttt cacgaaaaat atcttttttc gacactagcg
     3241 tttctctact tataatattg tacacagttt ttgtattatt gaaaaattac tcattattta
     3301 gcattaatcc acatcgtttt atcaatcgac cttcatacta aactacttta aagtcatctt
     3361 ccttctcatt tttcattaaa tcagatacat aagcccatgt acccgttcgt tgtaaataaa
     3421 gttcttcttg tagcagctcc cactttctct tagaaatcat aatgacgcca tcgttcaatc
     3481 catttttaga atccctagta atttctattt cagaatttga atgaactact tcttgcaaca
     3541 ctttacctat attttttttg aagtttgttg gagtaatagt atccaaaatg aacacctctc
     3601 ttctctatac ctctattatg aagcgcccat caaataaagc aagcgttgag gaataattct
     3661 ttcaacgctt gctttattaa gctttcattt tgtagtttga ctaataccaa taatctgatc
     3721 tctaaacaat cgaaaaactt cttgtggttg ataatagtct atatctattt caaatatttc
     3781 cgaaaacaat tcaaggaact tttctactga aatgataatg ttaccatctt cctcttggct
     3841 gttttttatt atttctgctt ctaattcaaa taaatctagg gacgaactaa aatcataaat
     3901 cctgtcatat tgaacaaaaa aatcaagaaa aagttgctca aactcttcct tatttatcac
     3961 tagtgtttca agcggaacaa ctttattttc atcagccatt cttccaattt cctcactttc
     4021 attttcaata atcacaaaaa tgacattaac ttaaaaacca ctaacaaaat aagacttaca
     4081 aattaaaggg taatccaaca aaataaccat tcgctctacg tactacgatt tggtattcct
     4141 gcctttcact aggtaattca taatagtaat ttattgtttc tgatttacca ctttcaatat
     4201 actctggatt atccaatatc atagcatact cataaagttc ttcaccattc ttattttcaa
     4261 tcgaccattg ttgcggacgt attggatttt ctgtatcatt tttcattgta taggtcactt
     4321 gtaatacacg atcatgttgc gcaaattcat ttctttttcc ggtcaattca acctttttaa
     4381 acgttatttc gaccccatct gcctgataag tttcacccat ttttaaatct ttagttacaa
     4441 tttttactgg tttttcttct ggaaaatatc tggctttaat tgttagatag cttccaaaaa
     4501 gaatcaatcc tagtagcaat aaaagaattc ctttaaagag ccactgaaac ggaaaataca
     4561 ctttaaacat ctttcccctc ctccgttttg catttgtttt ttagataata cgataacttt
     4621 tgatcaattt catccaactt tcccaccaca attgattcta tttcagaaat tttttgttca
     4681 attcctttgc tttcaggatt acttaatttt acatttgatt ggttagctaa cactggtaaa
     4741 tttgatacca tcacacgaaa catattggct tttgttccga aaatcccttt gttctgttcg
     4801 taaaaactgt caaaaattgt tttagtttct tttgaacaat acactttaat atacggatca
     4861 gacatgtgtt tttactcctt atcttttttt atttagggag tctgtaggga gcttgctccc
     4921 tcagaggttt tccccatcta acttgccaga aagtagccct gcgtaagcaa gggcggattg
     4981 actggcaagt agatgggtag ctggcgtatg ccctcaaaac cgcctctttc caattccctc
     5041 cgtatcgatg ggaaactgga aagtctactg gctaattcct ttagaaatcc atctcaaaat
     5101 ctcgttgtct cctttgctga ttttcaatca tttgttgcgt gaactcgtaa tcttttttag
     5161 cacgatacat tttcacatcg tcattcatta tgcgactgat ttgatagtga atctgtttag
     5221 cagaaggtgt atgagaacaa tttttaaagc cctcagctgg tagaaataat tctttctgat
     5281 ctgtcttttt aactaaaatt gttttacttt tagtactacg taaagttcta ggattcacat
     5341 cagtatgaag tttaatcgtg tattcttgat catcttctaa agatattgtg tttgcataat
     5401 gcctgtctac ttctcgcaag aaatcatcat atttaatgat ttttttagca ccctcatgat
     5461 cactaaactg cttttgtgca atggaatcta attccacaaa cggtacattt aactccccag
     5521 ttttataatc aactccacta gctaacgcta taatttgttc cgtatctaga tcaacaaaat
     5581 tattcatatg tgtttcttct aaattctttt caagtcgttc aaaaacagat tcaaatgaat
     5641 attttttccc taactttctt gcactaaaag tacgttcgac atactgactt ttttcttttc
     5701 tagattgttt ttctcttcct aaagtaatta gctcatctct acgctcatag aaatttacat
     5761 cttgtttgta aagaacatag ccaagtttgt tatttgtttt tgaaaattga taaacttcga
     5821 tgttgtattc ttttgctaaa tgatccgaaa aacgcacaaa tgaatttact tcacaattct
     5881 ccaacgtagc ttcaatcgca ttttgtacaa actcttgata attaattttt ccatttttca
     5941 gtttacttgg atcgtttgaa tagagattcg ttctcttttc taaattttca tagtactgat
     6001 tgtctacagg ttcgagtcca ttttcaacaa gcacctcatc gttaattttt cgcattcgtt
     6061 ctaaatcttt agggtcaata cgcaacctct tacaggtctc catatcaata ttattaataa
     6121 caagatgact atgaaaatga tctttatcat tgtgacttgc aacaataatt tgcgaatttg
     6181 gaaaagcttt ttctgcaaat tcgacagcta tttcgtgcat tttctcaata cttaactctt
     6241 tagaaatatt cttatcaaac gaatggataa catgaaatgc ttgtgctata tcttcagatt
     6301 tattcatcct ggttcttact aaattcattt cttgcaatgc ttcctcacta ttatcactac
     6361 aatttattcc tgagactgca acgcattttt ctttttttga aggattagct gcatattgta
     6421 atagcgagga taaatttttc ttgcgtcctt tcgatgttat agtagctccc aaagttgttt
     6481 cacctcatct tttaaacttg caataatttg aatcgagtga attaaattcc ggtcaacttt
     6541 ttgggaatac tcttctttcg tatcgggtct agtttgcttt tctaaccaat attgaaaata
     6601 atctttttct acttctgaca gtttgagaaa agaagatgta ctccgattgg actccaaaaa
     6661 aatgtcatct ggataactaa tcatcatttt ttttgctatg ctattcgcaa gttcatcttt
     6721 aaaaatgttg taacggctaa cgtcaaaact aaaatgcagg ctcttttctt tcttattttc
     6781 agattgttct ctaaaatatt tagcaatgat atttaatgct tttaccgctt gattcaaatt
     6841 gttaccaatt tgatttaact gtttgattag agctacccta tcatcttcag ccaataacgg
     6901 cggttcatat ttgagtccct ttaaccgctc acgacaaaca tctgctctag aaattcctct
     6961 ttgttttgca atcaatgtca gttcaagata agtcttttta tcgacacgaa aatttaaggc
     7021 ctcactatgt ttgcgatttt tttcgtacat tttttgttta tcatccattg attatcccct
     7081 cacatttttt ctagagagca acatttgaaa ttgctctcta tttcattttt catcattaat
     7141 tcttcttgaa tcatcagttc aatataattt gtgaccgtga ttttttcatt tgtcgctttc
     7201 tttactgagt cgcataattc ggcaagttct aagaaatcct gcgtactaat ctgtagaatt
     7261 aattgcatga caatcacctc tttatttttt aaatagacta tcaaacagcg gatttggttt
     7321 atcagaaata tcctgaaaca gtggattatt ttcataagta aatcgtggac gtttcttctc
     7381 cttcacttct tgttctacaa tcactttttc gttaggcctt tcttttcgtt cctcgataat
     7441 atttattttt tgcgtcttat ctccctgact tgcttcagct cttgtttcac gtacttctac
     7501 cgttcttggt tgatttgacg cactactttg tacattttct cgtacatcta ctgttcgatt
     7561 tccattttga ctacttgtag tattctgcaa gttttctcgt acatctaccg ttctcggttg
     7621 acttgtccca ctactttgta cattttctcg tacatctact gttcgatttc cattttgact
     7681 acttgtagta ttctgcaagt tttctcgtac atctaccgtt ctcggttggc ttgtcccact
     7741 actttgtaca ttttctcgta catatactgt tcgatttcca ttttgactac ttgtagtatt
     7801 ttgtaagttt tctcgaacat ctactgagcg cctatcttga ctacttgtag aattttgtag
     7861 attttctttg actgattttt ctaatccggt atttgaattt ttagcttctt ggattctttc
     7921 tttagtcaac gcttcttttt cgtccatatt agcgcctgta cgcccttcta cacgtttcct
     7981 taacatttcc tcacgattca ttgcttcagg cgtgtatttt gcttcctgtc gcttctgatt
     8041 aattaatact tcagcagact tagcttgacc gtgcatagca tttttacgtt cttcaatatc
     8101 ttgtttaact ttttgctgaa ttgcacttgc aggattaata tttgattcag cattattttt
     8161 cattgcttca cgcatacgtt cagaactaga cataattcta tcagcgtttt caactttagc
     8221 gccatctttt ctatccgaaa gacgtgttgg catagaatca gcatattttc cagtttcatc
     8281 ttctttgttt ttagctacta taggtttgct atttttatta atagcgtaca cggtaccatc
     8341 atctaaacct tctttgaatt tattcttggt atcattagcc atatcacgca tactcttaaa
     8401 tggttttgtt gctctttcac tggccattgt agctccatcc gaagctaagc ctgataatcc
     8461 tcgttcacga gcatacccaa gtgttttccc cgttttatta gctttttgag aaagtgattg
     8521 accaaagttg cgttcaggtt ttcgttcttc cccttccgct tgatcgtttc caccactatt
     8581 tttcccacct ttagatcggt tcataccacc tgttccttta cgaaacagca tagctcccat
     8641 gccaaatgct gaagcaagct ggccatagcc ttcttttaaa ccaatatcca cgccaaagta
     8701 acgcatgact gttccactac cctttatcag cacaataaca gcgcagacgt acgctactgc
     8761 ttttataaaa ggatttacag aagaaccgct acctgttccg agataattaa caaacattgc
     8821 aaacatagac aatccgaacc cttgaaatcc gactgtcaaa taacactgta aaatatctga
     8881 caaaacttgc tttgatcgtt ggcctgtttc caagtcagta gcaaatacta aaactccaag
     8941 tacgcgttta aacgctagct cgagtattgc tgtaattata atgaaagcac taaaaacata
     9001 cgctaccgct aaagcaatca atccaaaaga aatcccccat agatttgctt gatagcgata
     9061 atatcctgat ttaatgccct caacaaaggt cgacagaaaa ttatccgaaa atttggtagc
     9121 aacaaattca ttatcagaat tttcaactaa ttcataacgc agattatcag ctattgggtt
     9181 ctctttttcc attttgtcga gtacattatc tgtcaatact tgcgtaagat ccgttttcca
     9241 aaaattttct tttgttagat tgttccggtc attcgtttta tcgatcatgg aatattgatc
     9301 tgttttattg atataagcta aatcagtaac cccttgttca atcagcgacc acgccacgcc
     9361 ttcgtccgaa tttgtaatcg tagtcgtatc attataaaat gttttagaaa actctattcc
     9421 gctgtgaatc aacgttggca ttaaaagaat caatgaaatt gacattacaa cattcattcc
     9481 aacagatttt agatcaaccg ttcccttacc tgtaatcatt ttataaccaa taatcactag
     9541 tgaaagtatc attaagccaa caacaatggt acttaaaaca gcttgataga ctttgttcaa
     9601 tccggtactt tctacaaatc taaacaaatc taaaacttcg ggaatcatat tttgaatgcc
     9661 atcgacaaca gcgaacattg attttataac agaccagcct atccacatac cgaactggct
     9721 gtaaaaattt ccgttgtatt ttttcaaata attctggtaa agcggattag ttaaaaactc
     9781 agtttgactc tcagtatcat caaaccaatt gaaaatactg ccttctgcaa tgttactcgt
     9841 aaaaagaatc acagctatta gaacaagaaa aataaccggc aaccaatttg tttttctatt
     9901 agcaatcttt tttttaatta ttttcattgt tacctccctc agcccatttt cttaggctct
     9961 cagagagttt ctgagctatt tttctgtcaa ttatgatatg agttacgtaa tcatcattaa
    10021 cagcttttaa atcgagaaca atagtatttg agcagatact ctcggttaac tcaagagtat
    10081 ctgcgttatg atcaagacag tgaatcttat ctactagatg attttcaatc attttaaatt
    10141 ccctcttttt tttgtagctt gattagttct aacgctttgt tttttagaga agtaaagata
    10201 taattatttt tttgtttgat acgatgatct gtttttgctc ttcgtatcaa gtcacgtaaa
    10261 gtcattgcta gctgttctct aacatgatct acaaaataac tattattaca aatttcatag
    10321 ataattttca gttgaacttt ttgttctttt tcagcagatt taattgcata attgaatttt
    10381 ttgtaccagt tgtcgaattg acgataatca ctattgctag ccagttttaa gcaattcaca
    10441 atactttctc cccacatttg tttaagaaga ttttcttcaa taaatgtttc gatcaattct
    10501 tcatctaatt tattattttt agttgaaagt gattcaaaaa gatcatcatt atgtgattct
    10561 ttaatatctt tactatcttt ataagactta tataattcta gttcattttt tgaactatca
    10621 gcaaaattgt ttttgtctct tgttttcatt gatgtgtcgc atagttcaaa ttttgaacta
    10681 tcaacagaat attgatttgt tccatagttc aaattttgaa ctatcaagtt tttttgacta
    10741 ttttcttgat gggttatctt attaatttta taaacatcat ctttgcacag tacaggcttt
    10801 aacaaataat aaaaatttgg gaacatttgc attttatttt tattgaagcc attttttctt
    10861 ttcacaagaa gtttaacttt ttgaag
//
LOCUS       DASOUD010000082        10797 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.082, whole genome shotgun sequence.
ACCESSION   DASOUD010000082 DASOUD010000000
VERSION     DASOUD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.082"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            351..2051
                     /locus_tag="U4A29_002051"
     CDS             351..2051
                     /locus_tag="U4A29_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681169.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(2153..2986)
                     /locus_tag="U4A29_002052"
     CDS             complement(2153..2986)
                     /locus_tag="U4A29_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO0681170.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVTNEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3290..5455)
                     /locus_tag="U4A29_002053"
     CDS             complement(3290..5455)
                     /locus_tag="U4A29_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO0681171.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            5715..8009
                     /locus_tag="U4A29_002054"
     CDS             5715..8009
                     /locus_tag="U4A29_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEO0681172.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            8002..8667
                     /gene="pgmB"
                     /locus_tag="U4A29_002055"
     CDS             8002..8667
                     /gene="pgmB"
                     /locus_tag="U4A29_002055"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEO0681173.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            8660..9667
                     /locus_tag="U4A29_002056"
     CDS             8660..9667
                     /locus_tag="U4A29_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEO0681174.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            9689..10708
                     /locus_tag="U4A29_002057"
     CDS             9689..10708
                     /locus_tag="U4A29_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0681175.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         3264 a         2291 c         1963 g         3279 t
ORIGIN      
        1 tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg ttgtgaaaac
       61 tgcgttatgc tttgagtaat aagagcgcta gtcagaaaac agcttctcat attttctagg
      121 ctagtgagct tattaccgag aagcagctga ttgaacaccg tttaccgaga ggtcatgaaa
      181 aagctacttt gtttcgagta ttaagaaaaa ttctgtttca aaacaaggag atgcaacaaa
      241 acttatgtcg catcctctct ccttagaact ttcacttatg tttttcttgt aaaattcata
      301 aatattttca tgaattttct aatttcttgc ctacctctcg cataggatat atgatgaatt
      361 tatctaaagt ttgcaaagga gttcgacaat tgaaaaaata tggcccattt ttagcaattt
      421 ttttcattgc tttggcattt ctcatgcctc atttattcac acataaacta attttgggtg
      481 ctgatgcccc attccattac aatcggttct atgatgccgc gcaacaaatt aaatatgggg
      541 atttccatta ttttccttcc ctttatggat tccagcaaag tggcaggatc gtgaatgccg
      601 tttacggccc atttttcgct tatttacaag gattgatcgt gttgcttgca ggtagctggt
      661 ttcattatca gattctttca aatttcattc tttatcttat ttcaggattt tctatgctga
      721 ttttactgag atatgtcaat gttcgaaatt gggtaagtgt accagtagcc gttttattca
      781 tgagtacgta ttctattcaa tactggtcta tcaaccaagg attcactagc tgggggactt
      841 gtttttatcc tttatgtatg atccctattg ctgatttcgt ccttcataaa aagttccctg
      901 ttatcaaagt cgctgtctca gtgggactta tgacacagat ccatttactg tcttcgatca
      961 tgttagcttt gatgtatatc cctttttatc tttattactt ttttaaccag actgaaaaag
     1021 gaaaagcact gcttcaactg attaagtcgg tcttgcttta ttttgttctg acagcaaata
     1081 tttgggcagg attagctatt gtttacagtg ggaataagat tcttcctccc tttgttaaca
     1141 gagaaatgag tcaaaaggcg atagatctgg atggcagtta ttggctccat taccctaagg
     1201 cctttcctat attgatatta tcaggttttt tgctttttat tttctttcaa caaaagaaca
     1261 ccttattcca aaaaatcgtg ttgtacacaa gtcttgtttt cttttttctt tctactagct
     1321 tgattccatg gacgacattg atcgaaaaaa atgtgccttt tattagtttg atccagtttc
     1381 catttcgatt tttcgtgcca tttacgctat tgcttctact ttaccttgcc ctttcactga
     1441 accagtggtc tgaacaaaag tggttccgtc ttttaagtat atttggcatt cttatttttt
     1501 cagcacagac gatccagaac ttataccagc atctggaaaa gtgggaaaat gaaacatttg
     1561 tcagtcgaca tacttatcta ttcgatacgc cggaagaagc aaggaaaaca ttcttcagca
     1621 aagatatgtc taccagcttg ttcgttttcc aaaaaaccac acctgactat ctgccaatct
     1681 ataaggagac aaaagacaat aaatacgacc ggtataacga agaaatacta gaaaacgaaa
     1741 caacttttat aaaaacacat ccaggcggtc ttttgacaat caagtggaaa aacactgggg
     1801 acaaaactgt tcatatccct gttatcgtct acgatcggac tgtgctacag caaaatggaa
     1861 aaacattgac tgattatgaa gtaacagata tcgggacccc aatcgtgaag cagaaaaaag
     1921 gaatcaacga gctcacgctt cattatcaaa cgccaatcta tttctatttc attctttctc
     1981 ttactttgat tggctggttt accttgctat gtttatttat ttatcataga tacaagcttt
     2041 tacgtgctta ggcaaaaggg ataaatacat ccgatcaaaa aactctccaa acggtataat
     2101 cgtttggaga gttttatttt aatcagtttt tttatgttat atgttatatt ttttattcgt
     2161 tcacatctaa ttcgatttcc accccgtagt gatcactgat gattggacta tcgatcccat
     2221 caaaaatccg ttgatatttt ttaacctgca ttccttcagg gacgaaaata aaatctatac
     2281 gcaatttttc tgtattttgt tcccaaccat ctattttctt ttccacagta gctgcttttc
     2341 ctttttcatt cgtgacaaca tacgaatctt gaagaagaag gcaactttct cttacagtat
     2401 aataaccttg agagtctact ggattattga aatcacctag gaaaaaaagt ggtacgcgag
     2461 tttccaaaaa gtagttttcc aagtttttcc actcaaaata aaagccactt tttttatctg
     2521 tccaccaaga aaagtggcag ctcgagacaa aaatgtcctg atcatctatc tttgttttcc
     2581 caatcaagat cttgcgtgtc tgataatctt ctttcttttg agattgggag acggtaagga
     2641 cctgactttc caatgttttt ttcgtaagca gtgcatttcc ttcttcatat ttatcataac
     2701 cgatgtggct catttcccaa ctccaaaaat actcttttcc gtgttccttt aaaagctgaa
     2761 ctaaacggta ggcaaaatta tctttacgga taggtacttg attctttacc tgacagaact
     2821 tcattagctt cgcaggttct acttcttcag attccagtaa ttggttgatt tcttgtaaag
     2881 caatcacttc ataatcttct aacaggatct tatctgctaa ttcttgcaat tttttctctg
     2941 gttctggctc aagccagcta tgcgtgttga gtgtcatgaa tttcattggt tttctccttg
     3001 tcttcatttc gaaacaaaag ggttgtgaca aaatttttgt cacaacctct ccagcaaata
     3061 gtgttctcct gctaactcct cagtaataag ctcaatggac caggagctat tttttgtttg
     3121 gctgttttta tcacttggtg cagggcagct ttttcacgac ctctccacta aacagtattc
     3181 aaactgctgc ttctcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt
     3241 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctcct tacattttaa
     3301 tggagccgat cactttacca gctgtcactg tacctaattg cttgatcact acttcttcta
     3361 ctttatcgtt tgtaaatacg atcatgatcg ttggatcttt ttcttcgttt tggatcacat
     3421 caagatccat ctcagctaat ttcgttcctg ttcctacttc ttggccttct ttgacagcaa
     3481 tctcaaaagc tggctgattc atttggactg tatcgattcc catatggatc aaaacttcga
     3541 taccatcttt cgtttccagc ccaattgcat gtttgctagg gaaaatactt gtcacttttc
     3601 cagcgatcgg cgcaacaact tctcttgatg ttggaataac agcaaagccg tcacccatca
     3661 ttttttgtga gaataccgga tcttttactt cagataaagg aatcacttct ccagaagctg
     3721 cggaaactag ttcttgttct atacctaagt attgcttact acctgcttgt ccatttcctg
     3781 tagaaaccat ttctggctct ggaatctctg ctcctgattg cagcagatct tcgatatctg
     3841 atttcaagac atctgctttt ggtccataaa ctgcttggac tccattgtct ttgacgatca
     3901 atcccatcgc ccctgctcgt ttccaggctt cttcagtccc tactttttct cgatcattta
     3961 cacttacgcg taaccgagtc atacaagcat cgacatcttt gatattttct tttcctccca
     4021 gcaaatgaat gatctggacc acttgctgat ctgctgttcc tgttccgctg ttagatacaa
     4081 cggtttcttc actgtcattg tcgtaatttc cgttacgtcc cggtgtagca aagttgaatt
     4141 ttttgatcaa gaaatttgct aagaaatacg taacaacacc aaaagcgatc acacacaaca
     4201 cgaagttgat aagatctcca cctagacccg ctttgatagc taacggtgtc cgagtcagca
     4261 attcgatatt cccaaatgaa tggacccgca atggcaagat atctgccatc gcaaatgcag
     4321 ccccttggat cactgaataa atgacatata atggcacagc agcaaacata aacatgaact
     4381 ccaatggttc tgttacacct gttagaaaga cagccaacgc tgctgaaaaa tacattgatt
     4441 tatatcttgc tttcttatca ggatcgacat tacgatacat cgcaaaagcc attcccatca
     4501 agatacctga agaaccaatc atttgtccta ctttgaaacg agccggcgtc cagttatgca
     4561 acacaaactc atattgcgac atatcccctg cacctttaag attcactaaa tcagtggccc
     4621 aagccaacca taacggatct tgcccaaata cttgtgtccc cgcctgtgcg cctgataaaa
     4681 tctcatacga tccgccaagc tgtgtatagt tgataggaat cgtcagcata tgatgcaatc
     4741 caaatggcaa tagtaaacgt tctaatgttc caaataaaaa cggtgctaga atcggtgcgc
     4801 tttcttgtga ttctgcaatc cataaaccaa aattattgat tccagcctgt acgtttggcc
     4861 acaccacagc aagaatcaat gccacgattg ttgaccataa gataacaaca aacggaacaa
     4921 agcgctttcc attgaaaaat gacaaagcat ccggcaattt tctataatta tagtacttat
     4981 tataagccat tgccccgaca aatccagcga tgatccctac gaatactccc atattcaaag
     5041 ctggtgcttc caatacactt gtaaaaaatc ctttaaccat gatcttcgta ccgaacagag
     5101 tgtgggtaaa tgcatcttca ttcgcaagca tgtcacttgt gacaccaaag atagccccag
     5161 tgatccgatt gattaaaata aatgagatcc cagctgcaaa tgctccgcct gctctttctt
     5221 ttgcccaact tcctcctatt gctaaagcaa ataaaatatg aagattacca ataattgccc
     5281 aaccaatatt ttcaattact ccgcccgttg tgacaagcgg cgtccaatca ggattgatca
     5341 agggaattga tttccctata ctgatcatca acccagctgc cggcataact gctaccacaa
     5401 ccattaaagc ctttccaaat ttttgccaaa actcaaaact aaatatcttt ttcattccgt
     5461 ttagctcctt tacgcaatcg gtttcgttaa acgtattata gcgtgcgttt tcttttttgt
     5521 aaatgcttta ttttttattt ttctgtctat accattctat aaaacctata atacgtgtgt
     5581 tcccttctat aattttctcc taaaaattta tttttttaag cttttttcta tgaaatattt
     5641 tcttttgtct attgacttgt gcaatcggtt gcgtttatta tgaacgtata aaacagctca
     5701 ttaggaggag aaaaatgaaa cagatcaaac gactatttgc tattgatcca tggaaaatca
     5761 gcacgacaca attagataaa aaaaatcttc gactgcaaga atctttaaca agtattggaa
     5821 acggttatat ggggatgcgc gggaactttg aagaaactta ctcaggggac caccatcaag
     5881 gtacttattt ggcgggtgtc tggtatcccg ataagacacg tgtcggctgg tggaaaaatg
     5941 gttatccaga atactttgga aaagtgatca acgccttgaa ctttatcgcg atagatattt
     6001 cgatcaacga tcagctgatc gacttggctt ctttggaacc agaagacttt tactgggaat
     6061 tagatatgaa aaatggtgta ttatctcgta cattcacaat cactacttct actaataaag
     6121 tacgcatttc cttcgaacgt tttctgagca ttgtcaaaaa agaatcagct tatatctatt
     6181 taactataga aatgcttgaa ggtagcggaa cagtcaaact cgtatcaaaa ttggatggaa
     6241 atgttcaaaa cgaagacagc aactatgatg aacatttttg gaatgagatt gatcgtggaa
     6301 ctatagaaga aatcggctat ttgacgacta aaactattcc aaacaacttt gggattgaac
     6361 aatttactgt tactgcagca atgcgccatt tcgttaatgg aaaatcagtc atccctgact
     6421 atcacgaaga tactttagca ttacatggtt gcttgtctgc agaattatct caaggtgaga
     6481 catggtcagt ggaaaaagaa attatcgttc ttactagtag agatattcca gaagcgcagc
     6541 aagcagaaaa agctgtctca gaattaacga accagacaaa caattatatc caagcaaaag
     6601 aagagcaaac agcggcttgg gcagaacgat ggaagcttgc cgatgtggtc atcgaaggag
     6661 acgaagaagc acaacaaggt attcgattca atttgttcca actattctct acttactacg
     6721 gcgaagatga acgattgaat atcggcccca aaggattcac aggagaaaaa tacggcggag
     6781 ccacttactg ggatacagaa gcgtatgctg tccctcttta tcttgcttta gccaaacctg
     6841 aagtaactaa gaatctgctg aaatatcggc ataatcaact gccgcaagca atccacaacg
     6901 ctcagcaaca aggattaaaa ggcgcgcttt atcctatggt caccttcact ggtgtcgaat
     6961 gccataacga gtgggaaatc acattcgagg agattcaccg aaatggcgcg attgcctatg
     7021 caatctacaa ttatacgaat tacaccggcg atacggaata tttgaaaaat gaaggactgg
     7081 aagttttagc agaaatcgca cgcttctggg cagaccgtgt ccatttttca aaacgagcaa
     7141 ataaatacat gatccacggt gtgaccggcc caaacgaata tgagaacaat atcaataaca
     7201 attggtatac aaatacgatc gcagcttggg tcttgcgtta cacgcgagaa aattatttaa
     7261 cttatcaaaa agaagctgct atcactatca gcgaagaaga actagccaaa tggaaagata
     7321 tcgcagagaa catgtacttc ccagttgacg aagaattagg aatctttgtc cagcatgaca
     7381 cattcttaga taaagatctc atgcctgtat ccgatattcc cacaagcgat cttcctctga
     7441 atcagcattg gtcttgggac aaaatcctta gatcttgctt catcaagcag gcagatgtac
     7501 tacaggggat ttatttcttc aatgatactt ttacaaaaga agaaaagcaa aaaaactttg
     7561 atttctatga accaatgact gtccatgagt cttccctttc gccaagtatc cattcgattt
     7621 tagcagcaga attaggaatg gaaagcaaag ctgtagaaat gtaccagcga acagctcgct
     7681 tggatttgga taattacaat aacgacacag aagacggctt gcacatcact tccatgactg
     7741 gtagctggtt gtcgattgtc caaggatttg ctcaaatgaa aactgcccat gatcagttga
     7801 gattcgcacc atttctgcca aaaacttgga aagcttattc tttccatatc aattatcgcg
     7861 gacgtttatt gttcatctca gtcacagatg aacaagttac tttcgagtta ttagaaggtt
     7921 ttcctctaga aatttacgtc tatgaacagt cttattcttt agcggatcgt ctagaaataa
     7981 cgctaagaaa ggaggaagca aatgtttaaa ggggtattgt ttgatttaga cggtgtcatt
     8041 accgatactg ctgaattcca ttatcgtgct tggcgaaagc ttggtcagga aatcggcatt
     8101 acgatcgacc acacattcaa cgaacagcta aaaggcgtca gccgagaaga ctcactctct
     8161 ttgcttttag cttatggcgg aaaagaacac tctttttcaa aggaagaatt tgcagaatta
     8221 gcgaaaagaa aaaacgatta ttatttagag atgatccaga cgatcgaacc aaaagatgtc
     8281 ttccccgggg tcgtgccttt actggattct ttaaaagaag aaaaaatcaa gattgctctt
     8341 gcttctgcaa gtaaaaacgg gccattccta cttgaaaaaa tgggtcttac accttacttt
     8401 gatgctattg ccgacccggc agaagcaaca aacggaaaac ctgcgccaga tatcttcctc
     8461 cttgcagcaa aagctgtcgg actaaaagca gaagaatgta tcgggatcga agacgcgcaa
     8521 gccggtatcc aagccatcct ctcaagtggc gctcaaccaa ttggcgtggg acgtgctgaa
     8581 gaccttggag aggagatccc gcttgtccct gatacgactt ttcttacgat cgattacttg
     8641 aaagaaaagt gtcatgacca tggataatcc agagatctcc gtcgtaccag ttccagagac
     8701 tgaaataaag aaaatcacat tgaagaacga ctacttagaa gtcgttcttc tcaattacgg
     8761 tgcccgcctg caccaaatct tcgcaccaga caaagaagga aaatcagaaa atgttttatt
     8821 gtcttacgat cgtttcgaag atgttctaac agacaaaagc ttttttggag caactgtcgg
     8881 acctgtagcc ggaaggatcc gggatgcttc ttggggtgag catagattgg agaaaaactg
     8941 cggatcacac catatccacg gtggcacgaa aggctggtct ttccaatttt gggaagtaga
     9001 agttttcaaa actccatcat caataggtgt ggtcttttat ttaaaagatg aattttctac
     9061 ctatccagga ccaatcactg ctacgatcac ttaccgttta acaaaaaacg aattagagat
     9121 gacaacctct gctagtagcc ttgttgaaac catctgcaat ccgaccaacc atgcctactt
     9181 caacctttca ggaaatggaa caagagacat ctatgaacat cagttaacgg tctttttaga
     9241 tggtgtgctt gaattagatc aagaaaaact gcctactgga aattggacaa aaaaagaaaa
     9301 actgccaatc gactttagaa aatccccaac actgcaagag atccttgcat gttatccaga
     9361 tggactagat gatgtatttc ttttgcatca tcctaggctt tccaaaactt ccttacaatt
     9421 attcgagaag cattctggtc gccaaatgac tatagcaact tctaataaaa gtatggtatt
     9481 attttctacg actggattcg aagctgattt ctctgtcaat ggaaaacaga tgcattcaaa
     9541 ctacggatta gcaatcgaac cgcaagaaat ccctgatatc gttcatttcc ccaaatttgg
     9601 atcgatcaac ctccatcctg gtcaagaacg gatcagtcag acgatttatc ggttctctgc
     9661 acaataacaa gagaaaggtg tttttatcat ggctattacc gtaaaagatg tggcgaaaaa
     9721 agccggtgtt gccacctcga ccgtctctcg tgtcatcaac gatcatccta gtatttccga
     9781 gacaacaaag aagaaagtcc gaaaaattat ggatgaacta ggttatgttc ctaacatgac
     9841 tgcccgcaat ctaggaaaac gcatttccag tgccatcggc gtaatcttac cccctcttga
     9901 ttcaaaagag cggatcggta acccatttta tcttgagatc atggaagcaa tcaacgaaga
     9961 agcgcggaat tatgatatga ctacagcaat cgctactgct aaaaattttg aattgctgtt
    10021 agagaacgtc cagcgtatgc atttacaaaa acaagtagat ggttttattc tagtgtattc
    10081 agatagtcaa gatcctgtca ttgattattt ataccaaaac cacatccctt tcactttgat
    10141 cggacagcct tatcatcatg agaacgaaat cgtttatgta gataatgata accagcttct
    10201 aggtaaacaa gcaacagaat ttttgatcca gaatggacat aagaatatcc tgtttgtgac
    10261 aaacacgact catgaaagtt tgtatttcga acgatatttc ggttatcaaa aagcaatgat
    10321 gatggctggt cttcctgcgt tatccagtgt agtactagag catccggagg attatctgtc
    10381 ctttgagaaa acgttgaaag aaaacgaagc cacagcattg gtggtcattg acgatatctt
    10441 cgcccttcgc acgatgcagt tggcgcaaat gtacggctat gtcattccag agacactctc
    10501 cgtcattagc ttcaacaact cgatcttctc aacattaaca cacccttacc ttacaagtat
    10561 cgatatcgat atctccgaat taggacgtgt cgctatgcaa aagctgcgcg aattgatcca
    10621 tgaagaattg agcagtggcg tacgtttggt catccctcat cgcttgatca aaagagagac
    10681 agtcgttcct ttagaaaacc catactaaat agctgactca ctaaatacgc tgaccattta
    10741 tcctatggtc agctttttat gtaaaagaag atactacttc taacacattg tttatct
//
LOCUS       DASOUD010000083        10655 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.083, whole genome shotgun sequence.
ACCESSION   DASOUD010000083 DASOUD010000000
VERSION     DASOUD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.083"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            23..646
                     /locus_tag="U4A29_002058"
     CDS             23..646
                     /locus_tag="U4A29_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681176.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="U4A29_002059"
     CDS             1096..2145
                     /locus_tag="U4A29_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO0681177.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="U4A29_002060"
     CDS             2142..3023
                     /locus_tag="U4A29_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0681178.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="U4A29_002061"
     CDS             3026..3796
                     /locus_tag="U4A29_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0681179.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="U4A29_002062"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="U4A29_002062"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO0681180.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNGTDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="U4A29_002063"
     CDS             5107..6270
                     /locus_tag="U4A29_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO0681181.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="U4A29_002064"
     CDS             6387..6797
                     /locus_tag="U4A29_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO0681182.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="U4A29_002065"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="U4A29_002065"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEO0681183.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="U4A29_002066"
     CDS             9370..9654
                     /locus_tag="U4A29_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681184.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="U4A29_002067"
     CDS             9814..10050
                     /locus_tag="U4A29_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEO0681185.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="U4A29_002068"
     CDS             10047..10445
                     /locus_tag="U4A29_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEO0681186.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3719 a         1772 c         2254 g         2910 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgga
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaaactaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttag
//
LOCUS       DASOUD010000084        10651 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.084, whole genome shotgun sequence.
ACCESSION   DASOUD010000084 DASOUD010000000
VERSION     DASOUD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.084"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..649)
                     /locus_tag="U4A29_002069"
     CDS             complement(<1..649)
                     /locus_tag="U4A29_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO0681187.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMI"
     gene            974..1390
                     /locus_tag="U4A29_002070"
     CDS             974..1390
                     /locus_tag="U4A29_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEO0681188.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1387..1857
                     /locus_tag="U4A29_002071"
     CDS             1387..1857
                     /locus_tag="U4A29_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0681189.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1871..2644
                     /locus_tag="U4A29_002072"
     CDS             1871..2644
                     /locus_tag="U4A29_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0681190.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2658..3470
                     /locus_tag="U4A29_002073"
     CDS             2658..3470
                     /locus_tag="U4A29_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEO0681191.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3524..4489
                     /locus_tag="U4A29_002074"
     CDS             3524..4489
                     /locus_tag="U4A29_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEO0681192.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4626..7349
                     /locus_tag="U4A29_002075"
     CDS             4626..7349
                     /locus_tag="U4A29_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEO0681193.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7362..7994
                     /locus_tag="U4A29_002076"
     CDS             7362..7994
                     /locus_tag="U4A29_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO0681194.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8199..8426
                     /locus_tag="U4A29_002077"
     CDS             8199..8426
                     /locus_tag="U4A29_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEO0681195.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            8495..8761
                     /locus_tag="U4A29_002078"
     CDS             8495..8761
                     /locus_tag="U4A29_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEO0681196.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            8751..9020
                     /locus_tag="U4A29_002079"
     CDS             8751..9020
                     /locus_tag="U4A29_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO0681197.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(9199..10500)
                     /locus_tag="U4A29_002080"
     CDS             complement(9199..10500)
                     /locus_tag="U4A29_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEO0681198.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3577 a         1659 c         2102 g         3313 t
ORIGIN      
        1 caatcatatg gaagccaccg attaggtatt ttatgggttt agtcggaaat gtttcaagag
       61 caatttccat catatcgata atgccatgat gactacagcc agtaaagaca atcagaccgt
      121 cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca aatttatcat
      181 ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg atgatatgga
      241 agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc attttaagga
      301 tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa taatggggta
      361 aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat cttaacccgc
      421 cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc aaagtttccc
      481 ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag agatatttct
      541 tccctgagta gttaattaag agagataacc catgtccact tttaaagttc aaatttattc
      601 ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca tgaacgtgta
      661 ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa aaaatattaa
      721 tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat gtggcggggg
      781 attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct ttaaataccc
      841 acactttcat caacactata atttagggaa agaaaagtgt tggcatgata tagaaaaacc
      901 tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa gcgtaaggat
      961 aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg ctcaaggatt
     1021 aaagcaaaca gctacaatga ttattggaga tagtgtacca attcatgcct tgtcagcatt
     1081 tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca caatagatat
     1141 agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg atatgctcac
     1201 tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac ctttagttat
     1261 ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc ttattaaaga
     1321 gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta ctggaggaga
     1381 tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca ggtagtattt
     1441 tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga ggcagcgaat
     1501 gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa attaacaatt
     1561 ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa cgaaaatatc
     1621 atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta tcctaaagta
     1681 caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt tagtaatgcc
     1741 attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct tggcattaaa
     1801 caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat gttataaagg
     1861 aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt tttgtatttt
     1921 agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca gtacgcttgt
     1981 tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt tggaattgat
     2041 gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata tggcatcaat
     2101 tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat taacgattgc
     2161 cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga ttttgtcaaa
     2221 tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag caaggagaat
     2281 tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta tttttctagc
     2341 aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg agtggctaac
     2401 aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt tgttattaca
     2461 aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa ttactgctta
     2521 ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt ttgtcatggt
     2581 tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat tagatgactt
     2641 ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat cggcagttct
     2701 tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg aacatgggtt
     2761 ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa gatgaagcaa
     2821 tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct caaacaagtg
     2881 catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac caagaaaact
     2941 ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta tctggtgttg
     3001 gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga attaatttag
     3061 cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt gtaccttcct
     3121 ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa tacttgtcta
     3181 aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt gtggggttga
     3241 tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt caaatgggta
     3301 aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta ccattaggat
     3361 taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg atgttggcaa
     3421 tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa gggaataacc
     3481 aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt caaaagaaat
     3541 tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg gctgtggcgc
     3601 ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga agcagttacg
     3661 gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg tagatgagtc
     3721 aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg aagctaacgc
     3781 agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg actcaccgtt
     3841 aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg ctgctaaatt
     3901 agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag tagaaggtta
     3961 tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg cccaaaaaag
     4021 tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag atgaaaaagt
     4081 tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat caatttgctt
     4141 gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat ttttccacgg
     4201 accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg atggaaaaac
     4261 tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga aaacagaagt
     4321 tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct tagactactt
     4381 taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt cagaagcaag
     4441 agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa aaatagatca
     4501 aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt gattttatac
     4561 atatggtaaa ataactagta catatggtaa aataactagt acatattgtg aaagcaggtg
     4621 gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg aaatctacgc
     4681 cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat agaagtacaa
     4741 tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta aaaagttacc
     4801 cggttttatt tttacataga acagctcttg aagaactaca tatcacactg aataactccg
     4861 aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa
     4921 ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca gtcttatatc
     4981 ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa acctttctag
     5041 cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg gcacctttca
     5101 tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct gctttatttg
     5161 gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg ctagaaaaag
     5221 ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa gggcaagaga
     5281 aattatttac atttatggat acgggtgaat tctctcctat gggggataat agtattcgga
     5341 aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg actttcttac
     5401 caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa caacgtccgt
     5461 cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa attttagcta
     5521 aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat tttgaaggta
     5581 acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat atccaccaga
     5641 agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac agttataaat
     5701 tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac tatccagaag
     5761 aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt tttgaccaat
     5821 tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta tttttagaag
     5881 aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag gattacgaaa
     5941 aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt gattttatgc
     6001 gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc aactatctat
     6061 atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa accaaagatc
     6121 agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca aaaaaaatct
     6181 taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt ttctttatta
     6241 cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag agtattgtgc
     6301 tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt atgctgaata
     6361 agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa attgaagcaa
     6421 aattgattca attccttgga gattatcgaa cagattcagg cttgatttta ttggtagata
     6481 tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga ccactgttaa
     6541 tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta ttaaacaata
     6601 aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa aagcagttac
     6661 tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca gggattggta
     6721 gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt agagaattag
     6781 taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag actccttttc
     6841 aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat cagattccat
     6901 atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt cattacttgg
     6961 aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc aatttttcaa
     7021 ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta tcttttgtac
     7081 aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat aatcagctgt
     7141 ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg
     7201 atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg gataaaattc
     7261 atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat ttagagttgc
     7321 gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg ggagtaattt
     7381 ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga agaatgaact
     7441 tctttcaaga actggagata gatccaggat caaaccagat tgatgattat gtggggaaaa
     7501 cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga gaggaacttt
     7561 atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa gctttacgaa
     7621 aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg gcactggcat
     7681 cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt actgaatatt
     7741 tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc gaaatatatt
     7801 taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat tcaacgattg
     7861 gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa ccaatgcaaa
     7921 tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa caaattgttt
     7981 tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag ctattttgac
     8041 taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac accttttacg
     8101 aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt gttcgcaatc
     8161 cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag aaccaaaata
     8221 cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat actgatacta
     8281 ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg atccctgaaa
     8341 attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag agattttgcg
     8401 ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg tttgtactaa
     8461 cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga tgaacggagg
     8521 tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa aagaactggt
     8581 attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa atgctgtact
     8641 ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg aagtttccaa
     8701 agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa atgaattata
     8761 aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga aaccaagcta
     8821 aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg cgtcagcacg
     8881 gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga aactatcgaa
     8941 tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt ggacatagaa
     9001 gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa gtcaatgtat
     9061 aatcttttga attttgcata ataccacagg tagaaactac ccgatactat cggttggttt
     9121 ctgctttttt atggctcttt gtcaaatagg actgatagag tgagataaga acatctaagg
     9181 tagaattcct tttctacttt agatgttttt tgattcatag gtaatcagat tatttagtaa
     9241 gaaaattctg gtcttcattt tccgaaagct tcgaaagcca taagcgactc gtttcaatgc
     9301 tttgatgtgg gtatgcagat tttctatttt ggcatttgaa taaggcaatt cgagggctaa
     9361 acgaatgccg gattcatagt ttataaaagc tttttttata gaatgcagtt cttttggaac
     9421 ggaattgggc atcgttctta caagtttaaa gaaagtgtcc gcctctttgt cgtgaaacgc
     9481 ttgaagaatt tcttgatagt aaaaataggc gattcgtaat tcgtcagaga tcgaaagcag
     9541 acgatccacc acttcacttt cggtgagata cgcccaacca aaattgcgcc atttggtcca
     9601 tttgtcataa acaagcttgt cctgtcgttt gatcagaagc cgagacaacg cctttaattg
     9661 tcgataatat ttggcttgtg ttggatcgtt tcgatcaaag cttttcatga ttcgtacgcg
     9721 aatatcctca aatttacgaa tgagatgttt gacaatgtga aaacgatcca caacgagttt
     9781 tgcctttgga aaacattcct ttatcagaga ggcataagaa acattcatgt ccgtcacgat
     9841 gacctttacc ttcaatcgtg cgcgtcgttc aaattgcata aaataagaaa cgagagcctt
     9901 tttcttccgt gaaggaagaa tatccagtaa ttttccagta tcgccatcca tacaagaaaa
     9961 ggagtaggga ccgacttgat ttttcagtga acgaaattca tccattaata aacaacgcgg
    10021 taaccaattt ctttttactt ttgtcatggg gatcagagaa gcgagtgtcc gagaaacggt
    10081 cgaccaagaa acgccgcaat gcttagcaat tgtcgtcatt gcttgttttt cagttagctc
    10141 ttctaaaatt ttatagcgca cttgtttagc gataaagcag tttctatcga caatgtaggt
    10201 attcgccgta aaataggtgt ggcagccacg acagtggtag cgttgtttcg ctaaagcaag
    10261 ataagtcagt tggttcgcac atttattaag ttgaatgagc gattttttct ttccgttctt
    10321 aacgatcatg ccttcatgat tcatctgttt acacaaagga cagcgacgaa tttctttact
    10381 taacgtcgca gaaacaacaa gactcttgat attcttaatg ctcttcgatt ccacatttgt
    10441 gatgaaaatg tttttatctt tgattccaag tagtagtttg atatgatgaa gatcaggcat
    10501 acagaacgct cctttgattg tgttgtggtg attcaattct aacggagctt ctgtatgttt
    10561 tgttttttca tgctcaaaag ggaccgatag aatgattttc tatcagtcct agaaattata
    10621 gagccttttt tatttggtct tgaagaagaa a
//
LOCUS       DASOUD010000085        10510 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.085, whole genome shotgun sequence.
ACCESSION   DASOUD010000085 DASOUD010000000
VERSION     DASOUD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.085"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="U4A29_002081"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="U4A29_002081"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO0681199.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="U4A29_002082"
     CDS             1085..2005
                     /locus_tag="U4A29_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO0681200.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="U4A29_002083"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="U4A29_002083"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO0681201.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="U4A29_002084"
     CDS             3311..4789
                     /locus_tag="U4A29_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0681202.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="U4A29_002085"
     CDS             5066..7126
                     /locus_tag="U4A29_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEO0681203.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="U4A29_002086"
     CDS             7209..7778
                     /locus_tag="U4A29_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0681204.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="U4A29_002087"
     CDS             complement(7953..8759)
                     /locus_tag="U4A29_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681205.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="U4A29_002088"
     CDS             8897..10411
                     /locus_tag="U4A29_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEO0681206.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3726 a         1753 c         2071 g         2960 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt gggtaaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagca cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aaccagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag
//
LOCUS       DASOUD010000086        10088 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.086, whole genome shotgun sequence.
ACCESSION   DASOUD010000086 DASOUD010000000
VERSION     DASOUD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.086"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(554..778)
                     /locus_tag="U4A29_002089"
     CDS             complement(554..778)
                     /locus_tag="U4A29_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681207.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            969..3746
                     /locus_tag="U4A29_002090"
     CDS             969..3746
                     /locus_tag="U4A29_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEO0681208.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3827..4246
                     /locus_tag="U4A29_002091"
     CDS             3827..4246
                     /locus_tag="U4A29_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO0681209.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4258..4728
                     /locus_tag="U4A29_002092"
     CDS             4258..4728
                     /locus_tag="U4A29_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0681210.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4745..5524
                     /locus_tag="U4A29_002093"
     CDS             4745..5524
                     /locus_tag="U4A29_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0681211.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5511..6332
                     /locus_tag="U4A29_002094"
     CDS             5511..6332
                     /locus_tag="U4A29_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEO0681212.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6349..7398
                     /locus_tag="U4A29_002095"
     CDS             6349..7398
                     /locus_tag="U4A29_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEO0681213.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7411..8415
                     /locus_tag="U4A29_002096"
     CDS             7411..8415
                     /locus_tag="U4A29_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEO0681214.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9048..10067
                     /locus_tag="U4A29_002097"
     CDS             9048..10067
                     /locus_tag="U4A29_002097"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO0681215.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3389 a         1658 c         2099 g         2942 t
ORIGIN      
        1 taaatgaatt ctgcataaaa aatatatcaa gtaaatgttt atagatgaaa ctctatttac
       61 ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa aaaagtgggt
      121 tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa tatttattcg
      181 tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat ggtttggcgc
      241 agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca aaagctactt
      301 gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg tttctacctt
      361 gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa aaaggatggt
      421 gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc attatgccaa
      481 taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac aggaaagttg
      541 aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc aagataaaga
      601 tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa cgagtttgtt
      661 ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa gatccgatga
      721 tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc gatttcattg
      781 aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa actggaagga
      841 actgtacgtt ttttagaata aaaattataa gggataagta ggctgctgtc tataataaga
      901 tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt ttctatgatg
      961 gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag aaccagacag
     1021 cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta gctgatcatt
     1081 ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat gagcaactac
     1141 tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt gaaaaacaat
     1201 tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag gaacagcctt
     1261 ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc agtttgaaag
     1321 aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta ccacttttga
     1381 ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat cagtattgtc
     1441 tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt gcacaatatg
     1501 cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga gcgtacactg
     1561 gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc ttgtttttag
     1621 acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat cttgatcaag
     1681 gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt
     1741 ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga agaataccta
     1801 tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta gaactgattt
     1861 actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc agtggaccag
     1921 ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg aaaaatatcg
     1981 tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa atccatgtca
     2041 cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat acagcacagc
     2101 aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag caacagcccg
     2161 aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag aaaaataact
     2221 gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta tttgattttt
     2281 tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg acacaatcta
     2341 tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat ggaaacagtg
     2401 tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta ccggaagaac
     2461 atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct tatccatcta
     2521 gttatcacta tgtccaacga attctggaat taagtaagat caaattagat attgaagaca
     2581 caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa
     2641 aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc agtatggcaa
     2701 atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg ccactagatg
     2761 tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa
     2821 atggattgat catattagta gacatgggat ctctgaaaga aatccatcag ttttttaaaa
     2881 aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta gcaatcacag
     2941 taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag actgttcgac
     3001 agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa gcactcatta
     3061 ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta gaaaaaagct
     3121 tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta aaagataaga
     3181 aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc accacagatc
     3241 ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg gaaaagatgt
     3301 ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa atattcaatc
     3361 agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact attctagata
     3421 cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg cagttgaatc
     3481 gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac
     3541 gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg aagcagtgtc
     3601 aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag cattatagtg
     3661 tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa gatacagagt
     3721 ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa ttttggcatg
     3781 caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat
     3841 tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag agctgatcta
     3901 tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt ttgatttagc
     3961 ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg ttctgacgga
     4021 tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg atcatgtcta
     4081 tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt tggacgctga
     4141 agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt
     4201 ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg aagaaaaatg
     4261 attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt ttcatggaca
     4321 caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac gaatgagata
     4381 cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat caaatcaatt
     4441 gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt attcatcgta
     4501 gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat caaaaccatc
     4561 aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc ggtaaatatt
     4621 ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga agtagaaatc
     4681 cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga aaggaagaac
     4741 ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac aatcagaata
     4801 tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta caggaatcgt
     4861 gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag catttatcgg
     4921 atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga ttttagggac
     4981 ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag gtttgccgat
     5041 tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc caattatgaa
     5101 ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc atatgcactt
     5161 gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt cttatatggt
     5221 gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc aaaaggggtt
     5281 gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc gtttgctgat
     5341 caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt acttagagat
     5401 tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag tcaatattat
     5461 gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag atgaagaatt
     5521 ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt tggcgttctt
     5581 ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat gtttatgcaa
     5641 tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca gctttgaaac
     5701 ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta ggaatcacgg
     5761 ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg atcgatagta
     5821 tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt tggggaactt
     5881 tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat attttaggcc
     5941 ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat ctagcaacag
     6001 gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg
     6061 aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca atgactgcca
     6121 cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct gtgactgttc
     6181 aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt ggcatagtgt
     6241 tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta gctttctttg
     6301 gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat gtttacgatg
     6361 caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt
     6421 tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac gggttcatct
     6481 tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct tactgtcgat
     6541 attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat cgatactgtg
     6601 attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa aatgatacaa
     6661 aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact tgctttagaa
     6721 gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt tacaaaaggc
     6781 tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat aagtaaaaat
     6841 aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat aatcaaccat
     6901 ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag tttatttcgt
     6961 ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc taaagaagca
     7021 gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt agaagcttat
     7081 atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat tgaaggggaa
     7141 agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta cgttggagag
     7201 actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt agcaatagaa
     7261 tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt cgcttataaa
     7321 accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt tgatacagtc
     7381 ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc
     7441 aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat cgataaactt
     7501 tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta tgcttcctcg
     7561 atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt acagcatgct
     7621 gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt tattttgtcc
     7681 tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa gaaagtagga
     7741 gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct atgcgatttt
     7801 gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc agcagatcga
     7861 ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca gctcgaacgt
     7921 tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg cttatcattt
     7981 gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa tcaatgggga
     8041 gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc atcgaaatct
     8101 atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac aaccccagtc
     8161 acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc taaacttttg
     8221 ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt agaaggtatc
     8281 agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt gacacaacgc
     8341 atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg ctactatcgt
     8401 cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga gagcagcgat
     8461 aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa cacatttttt
     8521 gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc caaccatttt
     8581 ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt ccaagtacaa
     8641 cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa caaaagttcc
     8701 tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat ttagaaatgt
     8761 tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc ctaacctcct
     8821 aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca agcaaagcat
     8881 aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt tcaagtaaaa
     8941 acgataaaag aagtaaacaa acacttaaaa acaatggaaa ttagtcccaa ctttaagaaa
     9001 tgtatactat aatggaaatt atcaattatt tatttggagg aatatccatg aaacaaaaaa
     9061 tcgcagttct tggtcctgga tcttggggca ctgctttagc acaaacattg gcagaaaacg
     9121 ggcatgatgt ccgtatttgg ggaaatgtac ctgaacagat tgatgaaatc aataagtatc
     9181 atacaaatca gcatttttta cctgatttga cgatcccaga atcgattatc ggatataaag
     9241 aattggcaga agcagtggat aacgcagatg ctattttatt tgttgttcca acaaaagcga
     9301 ttcgttcagt cgcacaggaa cttattggta aaatgaatac taagccaatc attatacatg
     9361 caagtaaagg actagaacaa gatacgcata aacgaatctc agaagttttg gctgaagaaa
     9421 ttccagaaga aaaaagacag gcaattgttg tattatctgg tcctagccat gctgaggaag
     9481 tggcagtaca tgatatcaca acaattacag ctgcaagtat tgatcaaaaa gcagcagcct
     9541 atgtacaaaa actgttcatg aatgattatt tcaggattta tacaaataat gatgtgattg
     9601 gcgtagaaac tggtgcagca ctgaaaaata tcattgcaat tggtgccggc gctatccatg
     9661 gattaggatt cggagatgat gcaaaagcag caatcatgac tcgaggattg gcagaaatca
     9721 gccgtcttgg tgtcgcgatg ggtgccaatc ctctgacatt tatcggtttg agtggtgtgg
     9781 gggacttgat cgttacttgt acaagtgttc attctcgcaa ttggcgtgcc ggtaacttgt
     9841 taggacaggg acataagctg gaagaagttc tagaaaacat gggaatggtc gttgagggtg
     9901 tggcaacgac aaaagcagca gtagagctag ctcaacagct aggtgttgag atgccaatta
     9961 ctcaaacaat ctataatgtt ttgtataatg gagaagatat caaaaaagca gcaaaagaaa
    10021 tcatgttacg tgacggaaaa atggagaatg aattttctct tcgttaaata gatgatcaat
    10081 agttaatc
//
LOCUS       DASOUD010000087         9959 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.087, whole genome shotgun sequence.
ACCESSION   DASOUD010000087 DASOUD010000000
VERSION     DASOUD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.087"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(77..149)
                     /locus_tag="U4A29_002098"
     tRNA            complement(77..149)
                     /locus_tag="U4A29_002098"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="U4A29_002099"
     CDS             530..1099
                     /locus_tag="U4A29_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO0681216.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="U4A29_002100"
     CDS             1086..1946
                     /locus_tag="U4A29_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO0681217.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="U4A29_002101"
     CDS             2136..2840
                     /locus_tag="U4A29_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0681218.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="U4A29_002102"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="U4A29_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEO0681219.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="U4A29_002103"
     CDS             4677..5993
                     /locus_tag="U4A29_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681220.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="U4A29_002104"
     CDS             5994..6863
                     /locus_tag="U4A29_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEO0681221.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPVKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="U4A29_002105"
     CDS             6918..7727
                     /locus_tag="U4A29_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO0681222.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="U4A29_002106"
     CDS             complement(7830..8459)
                     /locus_tag="U4A29_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO0681223.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="U4A29_002107"
     CDS             8629..9921
                     /locus_tag="U4A29_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0681224.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
BASE COUNT         3269 a         1724 c         2137 g         2829 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gttaaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaat
//
LOCUS       DASOUD010000088         9001 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.089, whole genome shotgun sequence.
ACCESSION   DASOUD010000088 DASOUD010000000
VERSION     DASOUD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.089"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(47..697)
                     /locus_tag="U4A29_002108"
     CDS             complement(47..697)
                     /locus_tag="U4A29_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681225.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(797..1306)
                     /locus_tag="U4A29_002109"
     CDS             complement(797..1306)
                     /locus_tag="U4A29_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEO0681226.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1507..2376)
                     /locus_tag="U4A29_002110"
     CDS             complement(1507..2376)
                     /locus_tag="U4A29_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO0681227.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2396..3517)
                     /locus_tag="U4A29_002111"
     CDS             complement(2396..3517)
                     /locus_tag="U4A29_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEO0681228.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3519..3914)
                     /locus_tag="U4A29_002112"
     CDS             complement(3519..3914)
                     /locus_tag="U4A29_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEO0681229.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3933..4739)
                     /locus_tag="U4A29_002113"
     CDS             complement(3933..4739)
                     /locus_tag="U4A29_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEO0681230.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4732..5520)
                     /locus_tag="U4A29_002114"
     CDS             complement(4732..5520)
                     /locus_tag="U4A29_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0681231.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5534..6010)
                     /locus_tag="U4A29_002115"
     CDS             complement(5534..6010)
                     /locus_tag="U4A29_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0681232.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6082..8739)
                     /locus_tag="U4A29_002116"
     CDS             complement(6082..8739)
                     /locus_tag="U4A29_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEO0681233.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(8758..>9001)
                     /locus_tag="U4A29_002117"
     CDS             complement(8758..>9001)
                     /locus_tag="U4A29_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEO0681234.1"
                     /translation="EEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLF
                     NVLADQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
BASE COUNT         2801 a         1854 c         1247 g         3099 t
ORIGIN      
        1 ttccattttt acagttataa ctagtattat catgaaaaat agccttttat tgtctatggg
       61 aataataatt taaaattggt acttttttat taattagttc atcgcccaat aatctctcca
      121 gtttcttagc atctcctgac tcaaccggaa ttatccaagc ccagtctaaa taatttttta
      181 aaatctcatc atattttgca aatatttcat tgccatattc cgtccttaaa attatatttt
      241 cagtgccaga caagtgattt atattgagtt gataaaaaag atctccatct aaaaaaatat
      301 cattagaaga agatttcccc acagtaatga ccattccatc agctctatgt gttacataca
      361 aataattgta tgacctcatt tcacctaatg aatcaattat ttcttgccct ttagctctaa
      421 tatattgatc tctattacct atctcactct tatttaacaa aagttttttc atgtcaaatt
      481 taatcggctg ttttattttt gtaaattcgc ttctactact aatccaatcc gagattaatt
      541 tttcaacatg tcccagagta aactctgatt tattcgctaa atactttttt tgttgtatta
      601 aagacatctt tccccgaagc tcaaaattga gcctacatcg attctctatc tcttcaaaaa
      661 gcatcttatc ttcatcattc ttatattcat cattcataaa accaatccag ctcctcgatt
      721 aagataatat tttaatctca tacaaaaact agctctcatc aaaacaacca ttgttttgat
      781 ttctcatgaa atttctttat acttttctcc gattataaat tcctaacccc ttgagaaaat
      841 tacgcatata tttatctccg caaggcttaa aatttcgttg gccttctttt ctcaaaaatg
      901 ccccgatttc gccttttgat ggatagattt ctgcctcagc aaaaacgtct aaaatatcat
      961 cagtggtcat agacaaggcg atttttaatt tttttagcaa cacattgttg atatatttga
     1021 tttccttgct tttttgcggc gtgtctacta tcggcttatc tttttgcgga ccacgcgcca
     1081 agagaatcag gccatttagg aatgcgtcaa aaacttggtt gttgatggtt ttttcataca
     1141 cattattttc tgcattatct ttgttcttat cctgtacttt ggtcagtacc gcctgagctt
     1201 cttctttagt cagtggtaca ccgcctagct caaaagcttt aatcaaatcc gtatctttca
     1261 aatcaatcgc ataacgcaat cgcaataacc tatcattgtt attcatttgt tcctccgaag
     1321 tatttagtct ttactttctt acgtactttt tgtcttgcta agatttttag gatcaatgat
     1381 aaaaaccgca tcacttatga taaggcgctg ggtatcatga gtgaatgata ttgctgaata
     1441 caaatagtcc gaccacatta agttgatcgg acttcataag cattttattg actaatactt
     1501 ttttgcttaa gaattgattc taacatcttt tgctaaagtt aagcatccta tagttcctgc
     1561 attatctgct aacttaggtg tgacaatgta ttcttcgata tttggcgtat cgacatagcc
     1621 gttaattagt tctaaaaaac gtctttgtac tttctttttc agatgttctt gttgcatgac
     1681 accaccacca aaaattataa tatctggtga aagcatcagg gtcgtattac aggcacattg
     1741 tgcaatataa tccgcctcga tttcccagaa cggatcttct atagaaagat cttgtccctt
     1801 ggttcccgta cgcatctcga tagcgggtcc agatgccaac ccttcgagac aatcatgatg
     1861 aaaaggacaa gtaccagaga aagtatctcc tgaatgtctc ttaactaacg tatgccccat
     1921 ttctggatgg ctaaaccctt caacaaaaat cccatcttgt atagctcctc caccaattcc
     1981 agttccaaca gtgtaataaa caacactgga taaacctttt ccatgacctg aaacatactc
     2041 cccgtaacaa gcagcattca catcggttgt ccaggcaatt ggaatttcga aatgcttatt
     2101 caacgttcca acaaaatcaa aatttttcca ggcagttttt ggagtagagg tgatatgtcc
     2161 ataggtcttt gattcacgat ggatatcaat cggtccaaat gaccctactc caatgctttc
     2221 taattgttct ttatagtgat taaagaaatc aattaccaaa ctcattgttt cattcggtgt
     2281 agttgtagga aaactcactc tttctaaaac ctttaaatca tcattgccaa tggcacaaac
     2341 aaatttagtc ccacctgctt ctattgagcc atacatttct tactccaccg tccttttatc
     2401 taggaattcg ttcttccctg tctaatagtt tttcgaaaaa ctcatgtggt tccttttggt
     2461 accaataggc aacagaagaa atatcgtctt gacgctcata aagcttgatg tcatcatttc
     2521 ctagggtttg gaaagtaaca cgtaaatttt ctttgaaact aatggggtcg ggtaaatgcc
     2581 agcggtacaa cccatgcatc ggtaaagcat catcgccaaa accatgaact ggatttggat
     2641 taggttttcc agcactaaaa taatcccggg tgtgatctct tttagattga aaaggatagc
     2701 ccataaatag cgtattaaaa ttttgagcat atgccctttc tccaaagttc tttttatgga
     2761 aagcccaagc gccgccaaaa taatcttctg ctcctgtaga tgttattgta gggaaatctt
     2821 catctccatc tatataaaac ttaaattctc cttcccccca ccagtatctt tccaatgcgc
     2881 acaaagcaaa gtaggttcct acataatacc cagtgccttt gattccatca ataagggtgt
     2941 aatcttttcc ttttgtcgtg atgggttcac ggttccattt agcatgaaaa tacatcgcat
     3001 catccggtac ttcagtcaat gcataattaa atgtataaaa aatgtgctta ataaactcag
     3061 gatgttcatt cgttaaagta atcttagctt tttttcgaaa tggcatctca aaatagcaat
     3121 tcatccctga tgttggattc actacgattg gtaaggaatt cacttcgcat cttgtcccaa
     3181 acccattaca aaaaaaatct cctaatggac tttctacaga aggagtggat tcatcatccc
     3241 aataaatacg caaaattaaa tcacgtaaga cgaaactccc agcgattgtt ttgtctgtca
     3301 ctgtcatcca aaaatgacgt aaaattccag ggccttccat ttctgctaaa gtaattgtct
     3361 ctccagcatt tatcggaaca gaaggtgacc cttttctccc aattcctaga tgactcgctg
     3421 tcattgccgc tttccctttt tcaccatttt tattttctgg tgaaatagtt ctactttcct
     3481 ctcccttgtg gagccatata ttatttaaac aattcatttt attcgtcctc tatcctttct
     3541 tcactttttt gattattcat atagatgatt tgttgttgtg cctccgcaat aattgctcca
     3601 atatctaaat tatcatttcc taactgcaaa gcgattgaca ttgccaaggg aagattcatt
     3661 cctgcaatta cgtgcactct gtcagaaata taggtaaaaa acttttgatt tacacttcct
     3721 ccagccatgt cagttaaaac aattatttcg tctgtagtat ccactaactc tttaatttgt
     3781 tcatctatat catcaatttc ttcgacatat gcacataagc tactaatttg attatgcata
     3841 cctgttaaaa actctagtgt ctctttaaaa ccctcagcta gtattgaatg gctagcaatg
     3901 atcaatcttc tcataattca aatccttttc ctttacgcta atattccaaa tgcagccata
     3961 accatcgcaa aaacaataac gagtagaatc aaaatggtca tactgatttt tttcttactg
     4021 agtagataat atgctgcagc agtaataatt actggaatta atgttggtaa aattttgtcc
     4081 aacattggtt ggattttcat actaacttca ccaaaagtga atttaagtgg cgttgaaaca
     4141 ctgataactg aagcaattaa tgatccaact actgttagcc ccaatactga agcagcttca
     4201 gttaaataat taatcttttc accgtattca gtaacaatct tcattccctg cttataacca
     4261 aattcaaagc ctctcattcg gaaaaagaag attaccatga ttaaaatgaa ccataaaaag
     4321 attcctacag gattattttc taaagccata taagccgcaa ttgatcccat gatggttggg
     4381 atcatcgtca tcaaaagaga gtccccaata ccagcaattg gacccatcgt accaacttta
     4441 aagtcattaa tcgcatcttt tgcttctaat ccttgctttt cctccattgc aacacaagca
     4501 cctaaaataa tggctgccag ccaaggctgt gtattgtaat actgaaagtg attatttaaa
     4561 gcttcgatgt attcatcatc atctttgtat aattttctta aaataggtga caatgcgtaa
     4621 gtcacagatg caccttgctg tgtttgataa ttaaaggtac aaactgacat caaccaacga
     4681 tagttcgctt tcctaagatc ctttttcgtt acttgatatt tttcgctcat tttattcatc
     4741 ctccccaata aacccaacat cagaagctcc cgcagaagca gtgatttgtg catctttttc
     4801 agctctatga tacacattaa atgctaaagc gaatcctatg atcgccgctc caataatagg
     4861 taccttcaaa aaagcggcta agaaaaatcc aacaagtaaa taactaatgt atcgacctgt
     4921 aggcataaag cgtaacaaca tcgcaatacc aacagcaggc aacatttttc cggctaatgt
     4981 tagcccatcc ataaaccaat taggtaaaac atccaaaacg taattaacag cagactgtcc
     5041 taaagtaacg gcaataaata ctggaattgc tgcagataaa ccaaagaaaa ttggccccaa
     5101 ccacaaaaca ctattcattt tcttaaattc tcgtttattc gcatattcct gtgatttgcg
     5161 aacaacaaaa ccatttaaga ttttgacaat tacatctagt tgcaccccta acatacctac
     5221 aggtagtcct attgctaatc cggcttctac tccaagctgg ctaccaaatg ccgttgcaat
     5281 aattgcgcac gttccatagt ccggtacaga tgagccacca aaaccggcaa ctcctaatga
     5341 catcaattga actgtaccgc caactatcaa acctgttttt atatcaccca tcaaaattcc
     5401 tgtgaataat ccccaaaaaa ctgcgtaatt tacaaagatc atcggaatcc cataataatc
     5461 aacatgttta ataaatgcta atatagtcag cataataatt tgaaaaatag taaaatgcat
     5521 tatccttact cctttatcct aattttgatt gaatttcctg tgccttatcc gccggaacaa
     5581 attgtgtaac aatttctaca ccagaatcca acaatttttt atacgtgctg atattttcat
     5641 ctgatgcata ggctctagaa gatagctgta atgcattatc tttaacaaca gtaccgccaa
     5701 caattagtcg gtcaattcta ataccgttat ctactaaatg aaggattgtt tccggctctt
     5761 tcacgatgac aaatactttt tcctgatcgt atttcccctt tttaaaattc gcaatcgctg
     5821 tttcttcagt gataattgat acagccattc cagcaggtct agctaaacgc atacttgatt
     5881 tttggatgtc gttatttgcg acagaatcat cgatcaccat cactctgttt gctttagaca
     5941 ttggtgccca ttgcgtgaca ataatcccat gcaataaacg gttatcaact ctcgacataa
     6001 caatactcat aaatacttcc tcctttatct ttcattttct tacaagcaat taccatgcca
     6061 aaacaaaaag aacttgataa atcaagctct ttttacataa tcataaacgt atttaacctc
     6121 atgaatcggg atgtcgacac tataataatg ttcaacgaca ctgaaagatt gcttaaaatg
     6181 tgtctgaaat ttttgaaatt cctcactcgt ttcattgaaa taaatttcat cttccaaagt
     6241 attttgtttt actaatcgtt caattaagca actaatgtga acatataatc cgaaacacgt
     6301 attattatca aatgaaattc ctagatcatt ttgtaagatg tatatagcat ctgaaacccg
     6361 ttctaataat ttagtcggat ttaatatggt caattgctcc attaaattcg ttagactaaa
     6421 attcttcaga atattattgt tcaaaacttt cagttcatta tcattaaagt gtctattcaa
     6481 ttgattggag aatttactta tattcattcc taaaatcata tcttcaattg aaaagaattc
     6541 tacatcttct agatgtggat ttagtgttcc aacaataaaa agaatattgt tctctttacg
     6601 aagctccgat aaatagtttt cattcactaa agagtaatag tcacacgttt ctatgacaag
     6661 atcagtatcc cttggaaagc tatcttcaag tatctttttt aatttaaacg aagttcctaa
     6721 tccgcttgca caagaacaga caatcatatc cttcttaata tttgatgatt cataaatata
     6781 atccacattt tgattttctt ttacttgttc aagaatctgt ttaagcggta aatctaataa
     6841 tatctgattt gcaacatcaa tagccattct tgttgaaaca ttgtttagta gtccgaattt
     6901 tttttgagcg tctagttcca aatttagata aattttctcc agtgaaccca tatcaacaag
     6961 taaaataatt tcttcatatc cggtacactc ttgtaagtat ctattaattt tgtctgcaat
     7021 ctcactactt gaagtcatta gtggcatatc aatggcgtcg tatatatatc tctcaacgat
     7081 cctattagca gtacttgcta tacaggttgc cgttagatct ccatgagcca aaatcagtgc
     7141 taaacgtttt tgctgaaatt ctttactaca cattaactta aataaaataa ccaaaacatt
     7201 tttttgaatt tttgttaaat aaatatcccc tatttgaata aaacgattga ctaattgaac
     7261 ttctcgacta caccaactat taatatcatt gatgaaggaa tataatttat cgtccgatgt
     7321 taattggatt tgataaatcc aagaattcaa aaagatagac gttatttgaa gataataata
     7381 gtcaaattct atttgaaaat ctctctctat ttccttaaca tactgtctaa atcgaatctc
     7441 ttgataggtg ggatatgtta aagctgaatt attttggata tcaacattct tttgtctaaa
     7501 atatctatca tatttctcaa aaagctgctg cttatcagtg tctttcttcc acgtttgata
     7561 aagatactct ataagttcgt tgatattaaa aacttcgtct actttttcca aatcatccat
     7621 ttgaatatat tttaattcat tctgtctttt cataaccttc atgccgttga aattcaccca
     7681 ttgtggtaaa tgatacatct gaatataaag ttcttccaaa tcatctaaga aataaaaggc
     7741 attggcacag gtcaattgaa tgatattttt caaattgcca acattttcac taaagcgata
     7801 gtttaataaa aaatctaata attgttgaga tatcagtatt ttctttgcta gtctttcttc
     7861 ttctgattta aaaaatatct tgattaactg ttttctttca taaagagaac gactgtctaa
     7921 tgttggaagt gttagcttta caggtattct tctcaacaaa gttgataata gtttatcctc
     7981 aggtggttct gatgttgcaa atataatcca gcaacttgat gtgtaccagt tttcattatc
     8041 acccatccga tgaaatttcc tcgtatccat aaactgaaaa agtttctcct gacattcatt
     8101 gctcaatgca tgaatttcat ctaaaaataa gactccatta ttagctaatt ctgctaaacc
     8161 ctttcttcca tcttcggctc ctgtatatgc accttttaca tgcccaaaaa aattggacaa
     8221 gaaaagttct ggattattgg catattcact acaattaact gaaatgaaag atgccttctc
     8281 ttctatataa ttattggcca acaaatcctt atacacataa tttgctaaat agctttttcc
     8341 agttccagtc tcgccatgaa gtaaaatggg taagccattt ggcggatagt agattgctgc
     8401 tttaatttga gcaattacat ttcgcagact tccttgactt ccgataaccc tttctattcc
     8461 aaccctaaaa gagctatcat accatttttc cgcttcctct gaagagctaa aaatttgttg
     8521 atttggaact ttctccggta aagaaaagaa gattactgga cgagtattga tttttccaaa
     8581 ttctccatct tttacgaatt cattcaaata ttgagagaca atattacgac taacatttaa
     8641 tttttcagat attttctgtg ctgtattgtt cccccattta tttttaatct cagagttctc
     8701 tatgacataa ttgacaatgc gttcttttaa ttcttccatc acagcacctc ctcgacacta
     8761 ttcgaatttt gccgacagta tttttccata aatatgactt gctaaaaagg ctttgtttag
     8821 atcattcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga aaagatactc
     8881 tggcgaaatc gcatacccca aaatatcaac gattgttgca atcagatctt cttttgattc
     8941 ttcatctgaa agtaattcac gatacaacat cgtttgtttg cttactgtgt catattcctc
     9001 t
//
LOCUS       DASOUD010000089         8940 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.090, whole genome shotgun sequence.
ACCESSION   DASOUD010000089 DASOUD010000000
VERSION     DASOUD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.090"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            49..258
                     /locus_tag="U4A29_002118"
     CDS             49..258
                     /locus_tag="U4A29_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681235.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            465..665
                     /locus_tag="U4A29_002119"
                     /pseudo
     CDS             465..665
                     /locus_tag="U4A29_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1377..1982)
                     /locus_tag="U4A29_002120"
     CDS             complement(1377..1982)
                     /locus_tag="U4A29_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0681236.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2164..2499
                     /locus_tag="U4A29_002121"
                     /pseudo
     CDS             2164..2499
                     /locus_tag="U4A29_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2664..2891
                     /locus_tag="U4A29_002122"
                     /pseudo
     CDS             2664..2891
                     /locus_tag="U4A29_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3017..3988
                     /locus_tag="U4A29_002123"
     CDS             3017..3988
                     /locus_tag="U4A29_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0681237.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4128..5441
                     /locus_tag="U4A29_002124"
     CDS             4128..5441
                     /locus_tag="U4A29_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEO0681238.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5484..6350
                     /locus_tag="U4A29_002125"
     CDS             5484..6350
                     /locus_tag="U4A29_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO0681239.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6347..7195
                     /locus_tag="U4A29_002126"
     CDS             6347..7195
                     /locus_tag="U4A29_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO0681240.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7209..8681
                     /locus_tag="U4A29_002127"
     CDS             7209..8681
                     /locus_tag="U4A29_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEO0681241.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8698..>8940
                     /locus_tag="U4A29_002128"
     CDS             8698..>8940
                     /locus_tag="U4A29_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO0681242.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2829 a         1515 c         1831 g         2765 t
ORIGIN      
        1 ttaaaaagaa aagtgacaga taggaataaa aacttattaa atgtagttat gatctgttat
       61 atttcatttc taatactagg tcttccacaa gcagtgtttc ctgaaatgtt ttctggagac
      121 tggggtattg tcagctattc tatattaaca ttgattgcta tggttttagg tacttcattt
      181 tataatgctt ttgtaagtaa gcatgttgag gaatatgaag tattaatgta cttaaaagag
      241 aaaaaaacaa gtaattaata tgagaacacc acgcatagtt tttattttga gatattctag
      301 aatgaaacac attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta
      361 agttttttca tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc
      421 ccgttattga agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa
      481 tatttgagaa acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga
      541 tcacctccta ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt
      601 gccagttaga tatagatacc tttgaaacat ttgaagtgat cctagataaa attaaacaga
      661 attgatcaga aacaagcgaa gccttctctc ggttcgcttg ttttgctatt tattagttgg
      721 ctcagggcaa ctctaagctc gtttgaaaga aaaccgatgg ataatcccct tgtttgtacc
      781 actttagaat acatggatgt acgtattgcc attgactcct attagttact gaaaaaaaat
      841 ttaaacaagt tgagtattgc agataaatag ctccagtgag gtgaataact gctccactca
      901 aggcaataga tagctccatc cagggcaaca aactgctcca ctttcatatt taatagatga
      961 ataaccttct gtataataga aaggcatacc tttcggtatg acaacttata cagaatgtta
     1021 ttttattggt tcactatcga aaaattttgg agctttattt ttccggtatt tctcaacgaa
     1081 cgattagttt aactgtggga agctccagac atacgattcg agaagttact tctcgagcag
     1141 acaggaaggg ggtgactgaa ctaaccgctg atatgagcga tacttggctg taggaatatc
     1201 tttttccgga aaaacaaaca acagcacgag gttaccttcc tgtagactgg gaatatgttc
     1261 ataaagaact aatgaaaaag aatatgacat tgaaactcct tcatagagaa tatggggtac
     1321 atggaacata gaaacgaaaa tgcacggtag aaaagcgaat gaatatagta taaattctac
     1381 aaatcattcg ctttctttat tttatatatg gttggtcgag agagaccatt ttctttggca
     1441 atttctgcaa ctggaactcc ttgatttaac atgtccacca cagttttaaa gactaatcgt
     1501 ttttgcggat ctgttgagtt ctcagaatat tcaattggtc gccccttata tcgaccagct
     1561 tttttggcca ataatactcc ttgccgctga cgttcacgga ttttctcacg ttcgttttgt
     1621 gcaatataac ttagcaatga taaaaacata tcgaacatag cagtatctag gagttcattt
     1681 ccagtattaa aatctaaaaa ctgtgcatct agtattttta atgctacttt tttctgtttc
     1741 ataaaggtaa ctgtattttt tatgtcttca taatctctgc ctaggcgatc cagtgatgca
     1801 acaatgacac aatcaccttc tcggatataa tttagtaatt tttgaaactc aggtcgctgg
     1861 atatctttac cactggcttt ttcttggaat aatttatcaa tgttaaggtc ttccatcatt
     1921 gccaattgtc gatctaaatt ttgatcttgt gaagacactc taatatatcc aatttttgcc
     1981 ataaaaactc cttttaaaaa atgatataat ttacatgtaa aatttacttg tagttttact
     2041 gttatcatag catttttaaa aataaatttt aagtctactt ataatttact ttggaggttt
     2101 tgaatgaatg aaattaagcg caataaaaat tcttttggtg tttatttagg agagcctagc
     2161 tcggaacagt taaataaagg tgaagctcgt catgctctat gtcgaaatat attttatggt
     2221 agaaaaggca aactttatca aacttattac gacggtatgg aggaacagct caacgcatta
     2281 ggattagtaa ctaatgctat catatattgg aattcactat atttagaaaa agtctttgaa
     2341 caaattaaaa cggaagggtt cgactgttcc gaagaagtgt tacgcaagca atttccatta
     2401 attagtgatc acattaattt tgttggcaaa tatagtttcc gctatgaaaa agaattatcg
     2461 aatggaatac tacgtccgtt aaatatagag aattattgag aatgttcgtc ttttggttcc
     2521 taccgtgcat tttcgcacgg atgttccaga tactattttt gaatgtataa aattgaaaac
     2581 acaagaatac taataaatca acgattcttg tgtttttaat ttttcactac cgtgcatttt
     2641 cgtttctatg ttccatgtac cccaatcttg aaaaattaat taaagactta gataaagcca
     2701 ttgacgactt agtcgtggtg ctaccggagt atcaatgttt aaccagtatc ccaggtattg
     2761 gtcctgttta ggctgctgga atactcgctg aaattggtcc aattgaacgt ttttctgatg
     2821 aaataaaatt gacaaagtat gccggcctat attggcgaca gagtcaatcc ggtaaatctg
     2881 aatacgaaaa tttttttacg aaaagcttca cgtctaatat ctattttaaa ataaaaatat
     2941 tcctgctaat ttggaatcgt ttccttcttt tggtataata atacctgaaa ttcttttgag
     3001 aggagttgtt cgtgaagtga aaactactat gaaggatgtt gctgcgttag caggagttgg
     3061 tgtgggtacg gtatctagag taataaatgg agtaaaggtg aaagaggtta ccaaagaaaa
     3121 agtaatggct gcaattagtg agcttaatta tgagcctgat gaatatgcca gaggtatgaa
     3181 aacgaatagt agcaatacaa tagctcttat tttacctacg atttggcatc ccttcttttc
     3241 cgagtttgcc tactatgtag aggaagctct cagccaaaaa aattataagc tttttttgtg
     3301 taatgcgaac ggcagtgcgg aaaaagaaaa agaatatatc caaatggtca agcagagtaa
     3361 agtagacgga attataggta ttacctattc agacattgat aaatatgtat catcaaattt
     3421 accatttgtc agtatagacc gccatttttc ggaacaggtt tcttatgtaa cggcagataa
     3481 ttatttaggt gggcaaattg ccgctcaaga actacttact cgtaactgta aaaacttgtg
     3541 ctatgttggg ggtgtttcac cttaccctaa cgaaacaaac aatcgtaaag ctggttttta
     3601 caatatttgt gaggaaaata aaatagctgt gaaggtattg gatatgcctg aaccaattag
     3661 caatttagag gaacaattgc tcgctttttt ttcggataat cctgaaattg atagcatttt
     3721 taccatcaat gatttcatgg ctttggatgt gataaaagcc caaaaaaaac tagggaagga
     3781 agcacctaag gattttcaaa ttattggttt tgatggagtc aagctatcgt tgcaaaaaga
     3841 ctatttacta tcaacgattg tacagccgat agaggaaatg gccaaggttt cagtagaaac
     3901 actcatggcg atgattttaa atgaagaacc gcctaagcgt gtggtagtac cggttttctt
     3961 tcacgctgga gagacgacta aagagtaaaa aaccggttta taacgatttt tttgagatag
     4021 gttgacagaa agtgatttca atgatatctt aaaaccaatt aattggaaac gttaccaatt
     4081 aattgaaaaa taaaacggaa acgtttcaaa aaataggagg aacatcgatg aaaaagagaa
     4141 tgttagcaat tgcagcaatt ttagcattaa cagctggagg acttgcagga tgtggcagta
     4201 aggagaaaag ttcaggtagc agtgatccag gagaaattaa gatttgggtt cagttttctg
     4261 atgaaacgcc tgaaggaaaa gcttgggcag agattgtgaa caattttaat gatgaatacg
     4321 agggtgaata taaagctgtt actgagtata ttcctcgtag cggaagcggc ggtggatatg
     4381 aggataaagt aaatgctgca attacaacca atacccttcc tgatgttatt acgttagatg
     4441 gaccgaatac tgcagcttat gctcattcag gagtcattgc tccattagat gattatttga
     4501 aagatgcgga catggatgat gtattagaca gcattattca gcaaggaacc tacgatggca
     4561 aaatgtatgc gtttggtttc tcagagtcca gtgtgggtgt ctattacaat aagtcgctgt
     4621 tcaaagatgc tggaattaca gaagatcaat tgccgacgtt agaagatcct tggacttgga
     4681 cggaatttca agcaatttgc caaaagctga ctgataaata caaaacgcct gcaattgata
     4741 tgcaaatggc gtcgggagac gaaatgctga cctacgcata tacaccattt atatggtcga
     4801 atggcggcga tgtcgttaat gctgatggaa ccaaagccga agactatttt aacggtaaaa
     4861 aatccgttga agcgttggaa tttattcaag aattggttga aaaaggttac accacaatca
     4921 gtcctattga aaaaggattt gaaactggca aatatgccat gatgctgagc ggttcttgga
     4981 caatggcaga tttagaggaa aactatcaag agattgatta tggtattttg ccttaccccg
     5041 tttcagatgc aactggggag ttagtttcac caaccggaag ctggcagtta gcaatgactg
     5101 agaaaacaga gaaaaaggat gcttctgcgg cctttattaa gtttgcaacc agtacagaat
     5161 ctagtcgtat tatgagttta gggaacagtg ttctacctat cagaaattca acagttgatt
     5221 taattaaaga tgaagtgtcg gaaggaatgc aggtattaat taagcaaaac caactttctg
     5281 gacatgcccg cccagtggtt gtagcttatc cgcaagtatc tagagcattc caacaagcag
     5341 ttcaagatat tagctacttc gaggaaaacc cagatgtaca aaaagtagca gatagtcgcg
     5401 ctgctgaaat gcaacaggca attgatactt cactaaaata gaagtttcta aagggggcca
     5461 atcaagtgtt aaaaaaaccg acaatgaaag acaatttgat aggttacggc tttctagcgc
     5521 cagctttgct gctgctgacg atttttctga tcatacccgt aatcatggta atttattatg
     5581 cctttactga ttattatttg ttaacgccag atgctagaca atttatcggc ttagaaaatt
     5641 ttaagcgact gttcgctgat ccaatttttg taaaaagtat ttggaacacg gctaaatttg
     5701 ttgtttggat tattccggtc caaatagggg cagctttagg aatggcactt attatcaaca
     5761 aaaatcgtaa aggaaatatc ttttttaaag tagccttttt tgcgccagtc gtgatgtcct
     5821 tagtcgttat ttcgatttta tggttgtact tgttaaatcc taatgaaggt ctggttaata
     5881 cgctatttgc aaagattgga attgcttcgc agcctttttt gactagtccc aaacaagcaa
     5941 tgtatacgct tgttttcgta tcggcatggc aaggtgcagg atatcagatg ctgctgtttt
     6001 tagggggaat gcaaaatatt ccgcaagacg tttatgaggc agccgaaatt gatggttttt
     6061 ctaagtttca gcaatttcgc tatatcacca tgccattatt aaagcccacg gcgattttta
     6121 ttttacttac cacattgatt tcggccttta aactaatcat tcagccaatg gtaatgactc
     6181 aaggtggacc aatgaattct actatgacaa tggtttatta catttaccaa actggattta
     6241 cagaccgtat ggtaggctac tccagctcca ttgcgttaat ctttaccact atgattggtt
     6301 taattacaat tgctcaaaga aaattggtaa aggaggatga tccagcatga ccaagaaatt
     6361 tggaccgcta atgatcgtag aatattgttt gctgttcctt ttagcggcat tattcatttt
     6421 cccgatgctg tggatggtag tttcctctat gaagcctgaa gcagatgtct atcaaaattt
     6481 agcttccttc aaagcttttt tacctagttt gaatccagct aattggttta aaacttatca
     6541 agaggttatt tcccggttca gtgtgggaac ttatttatta aacagtattt tttatggttt
     6601 aacctttgcc tgtgggtcaa tcattgtaaa ctctttggcg ggttttgcct ttgcgaaggt
     6661 taatttttca ggcaagaaaa ttatctttgg agctttgtta gctctgctga ttgtcccgat
     6721 ggagacggtt ttaattccgc aatttaccat cgttaattca ctaggcttag tgaatactcg
     6781 cttggcagtg attttaccag cgatggccag cgcgtttaat atctatctgt ttagaaattt
     6841 ctttatcgcg attccagaag aaatcattga atcagcgaaa ttagatggtg caaatattgt
     6901 taaaattttc tttcacatca tgctgccaat gtctaaacca gccatagcca cagtaggtgt
     6961 cttatccttt atctccagct ggaatgatta tatttggccg ttaatggtct taactgacag
     7021 cagcaaattt tcaatgcaag tggcaattac tacgatcaat actacacagc cagtttatat
     7081 caatcaagtg atggctgttt taaccattgc aacgattcca ctgattattg tttatatcgt
     7141 tgcacaaaaa tatattctgc agggacttgg cggttctgga acgggtataa aataggagtg
     7201 aatagtgaat gttgcaaaaa cttttaaagg ccaatgagtt tattgagaaa aatgctcaaa
     7261 cagtcaatca agaataccgg aataaatttc atttgatggc tccgattggc tggatgaatg
     7321 atccaaatgg ttttgtttat tatcaagagg agtatcactt gttttatcaa tattatcctt
     7381 acgacagcat ctggggacca atgcattggg gccatgctaa aagcaaggat ctaattcact
     7441 gggagcattt gccagtagca ctggcaccag gtgaaagcta tgacaaggaa ggctgttttt
     7501 ccggcagtgc catagaaaag gatggcaagc tgtatttgat ttatacagga cacaatgttg
     7561 tggaagggca agttcgacaa gttcagtgtt tggcagtttc agaagatggc attcactttg
     7621 aaaaatatgc cggaaatcca atcatcgcag aagatcactt agcttctgtt gcaacgaccg
     7681 aagatttccg cgatcccaaa gtgtttcagc gaaaagaaga ctattttact gtagtcgcta
     7741 ctaaaactgc agatgctcgt ggacaaattg tgatgttcca ttccaaagat ttactaactt
     7801 gggagtttta ttccgtattg ctggagggaa ctccagaaca aggcattgta tgggaatgcc
     7861 cagacttgtt tgaactagat ggcaaagaag tcttgattct ttctcctatt caaatgactc
     7921 cacaaggcaa tgcttaccaa aatacgaact caactgttgc tttcatcggt actgtcgatt
     7981 ggcaaacagg caaatttcta gtagagaatt ttcatgaaat cgatggcggt ttagattttt
     8041 atgcacccca gacctgtgaa aacgaagcag gccaaagaat aatgattgct tggatgcaaa
     8101 tgtggcagcg taaaatacct acgaatgatc tgggccatgg ttggtcaggt tccatgacct
     8161 tgcctagaga gctgagggtg gctgataatc aattgttgca acggccagtt tcatcaattt
     8221 atcaagtaat tgatccgcag gaaaggattg aatgggcgaa gactgaagag aatcgcttct
     8281 tactgaagga cttagtatat gaccagcact atttacgctt ggaaatcaat ttaaaacagg
     8341 caacgaaact agaacttcac tatgcgaagg gacccgacca tacattactg cttaactatg
     8401 atgttgaacg ggagtgcttt actgcttcca gggaacacat gccgtatgat ttatcaggag
     8461 ctgaaaagga agtgttgaaa aagcgcagtg tgacgattcc tttggaaaat aattgtctaa
     8521 ccatcgaaat tttccgtgat acttcagcta ttgagctttt tctaaatgat cgtctgacta
     8581 tgagtacaac tttttatgag acagaaaaag gacaggattt tcttttgctg gcagacagtg
     8641 aagtaacctt ttcattggaa agcggcttaa ttaatctata gaagggagag ctcagtgatg
     8701 atttatggtt caatcgaagc aggcggcaca aaatttgtgt gtgccattag cgatgaagaa
     8761 atgacgatca aggaaagagt gagctttccg acaacgacac ctgaggaaac aataccttta
     8821 gtcattgact tcttcaaaca ataccaagca gatttagcag ggattgggat tggctcattt
     8881 gggcctatcg atatccatcg cgattccgcc acttatgggt acattacctc gaccccgaaa
//
LOCUS       DASOUD010000090         8937 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.091, whole genome shotgun sequence.
ACCESSION   DASOUD010000090 DASOUD010000000
VERSION     DASOUD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.091"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(307..1440)
                     /locus_tag="U4A29_002129"
     CDS             complement(307..1440)
                     /locus_tag="U4A29_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO0681243.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(1431..2099)
                     /locus_tag="U4A29_002130"
     CDS             complement(1431..2099)
                     /locus_tag="U4A29_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0681244.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="U4A29_002131"
     CDS             complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="U4A29_002131"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO0681245.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="U4A29_002132"
     CDS             complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="U4A29_002132"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO0681246.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="U4A29_002133"
     CDS             complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="U4A29_002133"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO0681247.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="U4A29_002134"
     CDS             complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="U4A29_002134"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEO0681248.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5475..6248)
                     /locus_tag="U4A29_002135"
     CDS             complement(5475..6248)
                     /locus_tag="U4A29_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO0681249.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            6413..6892
                     /locus_tag="U4A29_002136"
     CDS             6413..6892
                     /locus_tag="U4A29_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO0681250.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6949..7251
                     /locus_tag="U4A29_002137"
     CDS             6949..7251
                     /locus_tag="U4A29_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEO0681251.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7273..8730
                     /locus_tag="U4A29_002138"
     CDS             7273..8730
                     /locus_tag="U4A29_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO0681252.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
BASE COUNT         2551 a         1948 c         1561 g         2877 t
ORIGIN      
        1 acggttgtgt aaaaatatca ttttagagca tgaaaaaaag agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacatt tttgatcctt tttatttgat taattatatc
      121 cagctggata ataattttgg gggcaacatt tctatcttac tattatcttc ctaaagtagt
      181 agactttctt attgggaaaa attacaacaa aaaggagtgg cttagttgac aactcctttt
      241 tgtttacaaa tcaattggat atctataaat tgtattttct aacgaatttt atagacatct
      301 gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa atacttccaa
      361 tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg caacggattg
      421 aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat cctgcttgag
      481 cccatgcact cggtactgat gagaaaaaat tgatgcctgt ttctcctgag gaatgccaat
      541 tccctgatct tttacttcaa taatcacgct acccttttgg tttgaaatgg tgacaattac
      601 cagttttcct tcaccatatt tgattgcatt gtcaactaaa ttcgaaaccg cccgataaat
      661 ccaacgatct cgtccatgaa tcagggttgg ttgatcttca gtaaacgcaa acaaaagatt
      721 cggataattc attttgtagg catcacaaac ttccgcacag atcagtgaaa cgtctacgac
      781 ttccgtgcta agatcctctg agttattgga taatgtcaaa atatcgtcaa tactgtcggt
      841 catccgatct aaaagcatta aatttccttg atcggcatgt tgccgttcta tttccagatt
      901 cgtacgcaaa atagcgatgg catttttctg ctcgtgggtc agataactat ttaatcgtcg
      961 ataatcctcc aaatgaccat cgaacttatc ctttagctta ttaaaagccg catttaatgc
     1021 ctcgtccttc acagtatttt taggaacttg atcaataaac gttaaatcac gcgtaatcgc
     1081 taagatttga ttactgtaaa ttttgcgtaa aaccaaccaa agaataaacg ttaataacat
     1141 gacggcagaa caaataacca gcaccatcgc tggtaaatat ttaaatagtt cgtcctggta
     1201 agctttatct atcatcgaaa tagtcataaa ttgattgttg gaagatttcg aagcttccat
     1261 ctgcgtctct gcttgaaccg tcttaccctc ttccataaaa taaacatctt cacctaaggt
     1321 agatgcgata ctgattccct ccaaattatt tctcagaaag aagaaactca ctaaaatcac
     1381 gcctgtcacc aacagtgaaa aagccagcat cgttaacgtc attttaaaat ttaatagcat
     1441 agctggtacc cctttgctcg aatggttttt aagacctctt tttcagctgc ttcagccagt
     1501 ttctttttta gacgagccag atgcactcgc agaaccgacg aaaaaggatc aaaatcttca
     1561 tcgtagacat gttcagaaat ttcctcagac gaaacaaccg ctggatgacg ctgagcgatg
     1621 cacagtaaga tatcaaattc cttgggtttc aggaccacct ctgtcttttg atagctgacc
     1681 cttcgaccta atggatcaat cttcaaagca ccaattgaaa tctgcggcag cgcacgtcca
     1741 tgataacgac gaataaccgc gcgtattcgt gcttttaatt ctagtaactc aaagggtttg
     1801 actaaataat catctgctcc aaaatccaag ccttttgctc gttcttcaat ctcatcacgt
     1861 gcagtaataa tgataactgg actttcgact tcttcagcac ggagactttg taaaacagat
     1921 aatccatctt ttttaggcag atttaaatct agtagtatcc catcgtatga attgatgaag
     1981 gccttctctt ctcctgactc gccatcataa gctacatcta cagaaatatt ctcctgctct
     2041 aggccctttt ttacggataa aaccagctca cgattgtcct caaccaccag tacacgcatg
     2101 tatattcact cctcatacgg atttagtaat tacacttatg ctttttagca tagtacacgc
     2161 tcttcttcaa agtccacgtt ctttttgtga agatttgaag aaattcatac attctgttaa
     2221 aaaacacata attaagaatg attttactgt ttgaaaatac ctagaataga aaaagactgg
     2281 aacctttcct ccagtctcac tactttctat ttgcttggtt ttcttgtata tacttttcaa
     2341 agttcgttgg tttatgacca ataagcctcg ttagaatact gaaaattgcc atcaagtcat
     2401 taagtaccac atgtaagaaa atctctctaa agaaaaaccc cttcgtttta actcacttac
     2461 tattttagaa ttcgttcccc taataatgca gcatgatttt acgctttatg tataaaaaaa
     2521 gactgcaagc aattttgctt cacagtctga tttcttatga tagtgagctt gttaacactt
     2581 ccaccggtgt agcagtttct ttcaatacgg catttaattc ttcaatattt ttacgccctt
     2641 gcgtattcat ccactccatc gctgcttctt ttccatcaac gatatagctt tctacaccat
     2701 ctgcccaagt cgcacggccg caaagtacac cattgaaggt tgagccggca tttttcgcga
     2761 agcgcagtgt ttcttggaac aactctgctt ttacaccggc gcttaggaaa ataaatggca
     2821 ggttagtggc ttcagcctgt tccttgaaga agttagccgc ttgtgccttt gtgtacacga
     2881 cttcgtctgt cccatagcct tccacataat tcatattgac gggtacttcc acctttaaca
     2941 catctacgcc ataacgtgga tcagaaaatt ccttcatcat atcgttgact ttatgaggtt
     3001 tcttaatggc gtattctttc gaagtcgcat cagagatgtt cgcgtcataa gaaacaagct
     3061 ccaagaagaa gggtaagttt tcagcctgac attctgagcc gattcgttca ataaaggcat
     3121 gtttttgatt attgatttct tctgcttcat cgacatcata gtaaagtagg aacttacaag
     3181 catcggcgcc tgcttctttt aaacgtttaa ctgaccagat cgataataaa tctggtaaac
     3241 ggccgggagt tgacgcatcg taaccagttt tttcataagc taacagcaag ccggcatttt
     3301 ttgctctaac gtcagctgct ggcaagccat attcgggatc taataaaatc gatgaggcat
     3361 atttcgttaa ttgtgaagaa acaatttctt taaattcaac gatttctgat tcttctgctt
     3421 cgctgccctt gaattttgtg atcatcttct tcaaagcccc gcgttgatca attgctaagg
     3481 ccccaatcac tccatcttca ttgcatagtt gcttgatata ttgacgtttt tcttcgttca
     3541 tttccgtatc ctcctacact ttctccacta agatttgatc atatagtttt tgataattgg
     3601 ctaattggac atagccagtc gtcgcttcct gagcattcag cattcctaaa acattaccct
     3661 gttttaacac ggcttcatcc gaaagacctt gttgaatcgc agcggtcaat ccggcaacgg
     3721 tcgcatcacc agagccaact ggattgacga catcaatctt tggaattgtc acgcaataaa
     3781 aagtctcgtg atgtttggca aaggctccgt cgccccccat tgaaaccact atccattcaa
     3841 tttcatcaaa catcgaatct gacaaggcgg cttttaaatc tgataattct gttgagattt
     3901 ctcgtcctag cagaccagcc agctcttcgg tattgggttt tattagctta ggctttacac
     3961 tgcctcgcaa cacttccctt aatgctgtac ctgaacaatc taaaacgaca ggctttggct
     4021 gacaaacttg cagcatttgt cgataaaaat cagcaggcaa tccttctggc aagcttcctg
     4081 aaaaagaaat gatttcggct ttaacagcta atttttcgaa atgttgcaag aacgcccttg
     4141 cttcttgttc agtaataaca ggtccgtttt ctaggatttc cgtctgtttt ccgtcatgta
     4201 aaacagcgat gcaattacgt gtctctccgc tgataggtaa aaagtcatga gcaatctcat
     4261 tttgagtcag gtccttttta atctcttcac ctaaaaaacc gccgattaaa ccactagcaa
     4321 tcacctccgt atctaattgc ttcaagatac gtgtcacatt taggccctta ccgccagctg
     4381 ttttgcgaac atttcttact cgattgactg tgtcgagctt gaaggtttct aatggataag
     4441 aaatatcaat cgatggattc atcgttattg ttaaaatcat ttgtccaccc gcttaatcgt
     4501 ggtattcgcc acgatcccat ttctcgataa attcatcgaa gaaatgcgga tcagactgct
     4561 tgtcactggc tttttcgacg tgatcgattt tggcgatcaa ttttttgttt tcttcagttt
     4621 cttgataatc ggcattgatg aaggcctcta atatgtcaca gatcaaaaat tctcccgtaa
     4681 tccgagcgcc aaagccgatc acattggcat ttagttgttc cttcgcatag atagcagtcg
     4741 tcatatcgcg aaccagcgca ctgcggatac caggtacttt attgacagcg ttgttaatcc
     4801 caacacctgt tccacacaaa caaactccta gatctgcttc tccgctagtc acggcctctc
     4861 cgactttttt tccgtagatc ggataatggg ttctggtaaa gtcataggtc cccatatcca
     4921 acacttcatg tccttttgat tttaagaaat ctgatactgc catttttgta tctgtgacga
     4981 tgtggtcaca gccaattgcg attttcattt cattcatcct tcctaattac gccattttat
     5041 ttaacatgtc gacacgtact tgatgacgac caccatcata atttgcttgt agaaattcct
     5101 taacaatatt caacgccaac tcatcgccaa caatttttga tccaagtgtg atcatccgag
     5161 aattattatg tttacgggtc atatacgcac tgcgctcatc cgaaacttct gctgctacca
     5221 ttcctttaac ctttgttgcc gtgataaagc tgccagcgcc gtagccatca accgcgattc
     5281 ctaaactacc ttcgttttca ttcagatcat ttgcgatcgc caaagttgat tccacaaaat
     5341 cttctgaagc cgtttcactc ttatctacta ctgtaatacc ttctttgatc aattttttct
     5401 tgattaattc ctttaattcc atgccgtcac tgtctgaacc aattactaca cgcatttttg
     5461 tttcctccta ttttttactg aatgatttta gtgaattgct gataataact cttcagctcg
     5521 gctgaaagag tagaatccgt aaataatttg tcgatcttgc gcaaatcata gaaattataa
     5581 aaatcgctat ggtcgaattt gtttgcatct gaaaccaaac atttttctgc cgcattatct
     5641 aaaacaattt tttgcaaggt accttcttca atactgaaat tcgaaacctc cgaacccttc
     5701 aatccattca ctccgacaaa agccttctta ataccgattt tttgaacagc ttcattggca
     5761 attgaaccga caaaggctcc agtcttttcc cgatatagcc caccgatcaa atacagttca
     5821 aatcttttta tttcacttaa tagattgaaa accggcagac tattggtaac gattcggaga
     5881 gatgacggct tgatgtatcg tgtcattaat tcgatcgttg tacccgatcc aagaaaaatc
     5941 gtgtcgtctt cttgattgat atgagctgcc gcatttttag cgatgtattc cttctctttt
     6001 gtatgcagct tctttttttc agtatgagaa agctcatttg cttctataga agtcgacatt
     6061 tccagataat cgattttttg tgcaccgcca tgaacgcgta aaagcttttt cttctgcgcg
     6121 agttcctcta gatctcggcg gatcgtcata tctgaaacat tcagtacttc ctgaataaat
     6181 ttgactgtta tcgtcccata gcgatcacac atcgctaaga tcgaacgctg tcgttctgtt
     6241 ttcagcatat tctgtacctc cacgacctta taataaaaca tttacgaaca tttttcaaca
     6301 catattgttc gtttattttc gtttatgcgt tgacaatgat tttgtaagcg gttatactca
     6361 acttgtaaac atataaataa acataaacga actaataaag gagctgtttt gtgtgaatga
     6421 attctttttc aaagacactg tctatgtttc tgacaagcag tcgcaggaag aagtgttcga
     6481 ggaagtctat caagatctgt taaagaaaga attggtaacc gaagattttt tggagaacct
     6541 tttagaacgg gagcgtaatt atccgacggg attagatctc tcacctgtct ctaaaatatt
     6601 gccagatatc gctatccctc ataccgaaag tgagtttgtg cgaaccaccc gtatcattcc
     6661 aatcaaagta aaaaatgaaa tccctttcta caacatgatc tcgccaagcg atcagatttc
     6721 cgcttcattt cttttcatga tcttgaacga aaatggtgaa gcacaggctg gcatgttggc
     6781 taatatcatg gatttcatta attctgttga cgaagaaaaa ctgttttcgt ttttcaaatt
     6841 agaggacaca gaagcgattt atcatttttt agaagaacat ttttatatat agaagtctct
     6901 gcctaaccgc aggataaaaa ataaacttat tttaagggga gaattattat gatcaaaatt
     6961 ttagcagcat gtggagcagg cgttaattca agccatcaaa tcaaaagcgc tttggaaact
     7021 gaattgaaaa atcgtggcta tcaagtcacc tgtgacgccg ttatgatcaa agatatcaat
     7081 gaagaaatgc tgtctcatta tgacattttc gcacaaattg ccaacaccga cttgggtttt
     7141 gacattaaca tccctgtcgt tgatgcaggt gctattcttt atcgtattcc tgctatggct
     7201 gagcctgttt accaaagtat ggaaagcgca atccaatcat taaacaaata ggactaaaga
     7261 aaggaaaagc atatgaatac aattattgat attgccaata aagtatttac ccccctaatt
     7321 aatttaggtg cggcaccgtt aatgaccatc gtattaactg ttattgcttt attttttaaa
     7381 gtaaaaccgt cacgcgctct tgaaggcggg ctcaaattag ggatcgccct gactgggatc
     7441 ggagctatta ttggaatttt aactactgct ttttcagacg ctatgacagc ttttgttgaa
     7501 cgcacaggta tttcattaaa cattacagat gtcggttggg ccccactagc aaccatcaca
     7561 tgggggtcgc catacacgct ttatttttta ctaattatgg tgatcgtcaa tattgtcatg
     7621 ttagcaatga aaaaaaccaa cacattagat gtggatattt tcgatatttg gcatttagca
     7681 attgttggtt tgttcgcgat cttctgcggt gctaatctgt ttattgcttc tgcattagtt
     7741 attttcattg gtgtattaaa aatttggaac tccgacttaa tgaaaccaac cttcaatgac
     7801 ttgttaaatg cgccagacag caacccgatg accacgactc atatgaacta catgatgaat
     7861 ccgattatta tggttttcga taaaattttc gacaaaatct tcccttggtt ggataaatat
     7921 gatttcgatg ctgccaaatt gaatagtaaa attggcttct ggggatcaaa attcgcgatt
     7981 ggaatttatt tggggatttt tgttggatta ctagcaggac aaacaccaac gcaaattttc
     8041 tcgctagcct ttaccgctgc cgtctgtttg gaattgttct ctttgatagg cgcttggttc
     8101 atcgcagccg tagaaccgtt atcacaaggg attaccgatt ttgccaataa acgactaaaa
     8161 ggacgtacca tcaacatagg tttagactgg cctttcatag caggccgcgc tgaaatctgg
     8221 gctgccgcta acgtattagc accaatcatg ttgctagaag ccatcatctt accaggaaat
     8281 aaattattgc cactaggcgg aatcatcgca atgggtgtta ccccagcact attggtcgta
     8341 actcgtggaa aattaatccg tatgatcgta atcggcgcga tcgaattacc attattctta
     8401 tggtccggca ccttgatcgc cccgttcgtt actcaaacag ctaaagcagt cggagccttt
     8461 ccagccggct tgaacgggtc cgccatgatc tctcatacaa cgatggaagg cccgattgaa
     8521 aaattcctag gttatttagt cggtaatgct tctcaaggac aaatcgaatt tgtgatatac
     8581 gctgccttag cattaggtgc atacctttta atcttcattt ggtacgctcg tcaaatgaaa
     8641 aaacgcaacg ctgcatacgc agctgaaaaa gaagagaatg catctccggc aacggcgcat
     8701 aatatgactt atgcagccga agctaaataa gctaaaaaca ccccgaatgt ctttagactt
     8761 cggggtatta ttttacctat aaaaaataaa tagatgtaaa atccatttca aaaagcatag
     8821 cccatctctt gccacaccca ataaagaaat tatcaacgtt tatgcttttt ttaactcctt
     8881 taagcttgcc tttgttggta ttcctcaacg attctttttg tgttcttgtt tgttttg
//
LOCUS       DASOUD010000091         8785 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.092, whole genome shotgun sequence.
ACCESSION   DASOUD010000091 DASOUD010000000
VERSION     DASOUD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.092"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            90..737
                     /locus_tag="U4A29_002139"
     CDS             90..737
                     /locus_tag="U4A29_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681253.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(830..3109)
                     /locus_tag="U4A29_002140"
     CDS             complement(830..3109)
                     /locus_tag="U4A29_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO0681254.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3387..5210
                     /gene="cadA"
                     /locus_tag="U4A29_002141"
     CDS             3387..5210
                     /gene="cadA"
                     /locus_tag="U4A29_002141"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEO0681255.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5257..5436
                     /locus_tag="U4A29_002142"
     CDS             5257..5436
                     /locus_tag="U4A29_002142"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO0681256.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5773..7713
                     /gene="thrS"
                     /locus_tag="U4A29_002143"
     CDS             5773..7713
                     /gene="thrS"
                     /locus_tag="U4A29_002143"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO0681257.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7937..8785
                     /locus_tag="U4A29_002144"
     CDS             7937..8785
                     /locus_tag="U4A29_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO0681258.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
BASE COUNT         2767 a         1568 c         1874 g         2576 t
ORIGIN      
        1 tttcttttct aattcaacct gtggtatgat gagagcataa gtatcatgtg aaatgatatg
       61 aagtatgtgg aagatggacg gggaagaata tggaagcaaa aatcacttta gaaccttttg
      121 aacgtatctt aagcggttac cgcaaagtag aagagttagc tgtgaacgta actgattgtt
      181 caaaattagc gcaaaaatac gcaagattcg gcgttgaagg ctatcgatta ggaaactatg
      241 tcggtacagg gtatctgaac cgttatttag agtgtatggt ggaccgcgca ccaatgctga
      301 tttatagaca aaattatctg attccgctgc tttttcgtcg ttctgattca gcctttcggt
      361 tatttgaaga agagtatcgg atggaggctt tctttctact attagagtgg agcttgaaac
      421 atcgtcctga aaaaattctt atcgaaagaa atgagaagat agacacgaaa aaaaataaag
      481 tgatcgatag tgcataccta gcatttcgtg tatcagagat tttggattgt ggcggctatc
      541 caatcagcaa tttccaatca attgaccaat tcattgaatg gaatcgaatt taccgattga
      601 tcgacaatgg cgggattggc cgtcatagca aggtatttga cccagaatat ccagaaaaca
      661 tggaagaact gaaaatgatt atttcgcttg tcaaattaaa atatcctgaa acagatttgg
      721 atttatatat agaataaatg accttaaatg attgaattac tttttctgca attattattg
      781 taaataaaaa gacaactgct aacattaatg atagcagttg tctttttact tacagtactt
      841 ttttctcata agatgaaata tccatattgt ttaacaaagg actttcatta ttggaggtaa
      901 aaagggtcaa ttcatgcatc gcacgtgtac acatggtgta aaggatcaat ttgtcttgct
      961 gagaagaaaa atgttctgca tctacatccc acaagaaaac tcggtcaaac tccagacctt
     1021 ttgctaaaaa ggctggaagt aaaataatgg aacgcttcat aaagtcactc tcatttaaaa
     1081 tcagctggac ttgttgtttc aactcgtctg gcagttgagc ataaagagct tcgcattctt
     1141 cttgtgtttt agctatgatt gctgtacgcc aataccgggc ttttccctga gtcgttcgaa
     1201 tggtatctgc taaccaattc aatttgatat catctgttgt tccttggatg agcaaaggaa
     1261 ggtctccttt tcttgctgtt gtttctactt gatgttcttt cacaataaaa tgattagcaa
     1321 aatcagtgat ttcttttgtg gagcgatagc ttgtcgttaa ttggaaacga gtgacttcac
     1381 ggtccacgaa aaggtcttcc aatgatccaa cgatcgtttc atttccaaag acattttgat
     1441 ttagatctcc acagaaagtc aaatttgctt ttgggaacaa ggcttgcagc aatgccactt
     1501 gtgatggtgc gaaatcctgc atttcatcaa taaagatata gcgagctttg tgtgtaaccg
     1561 tcactggata tgttcggcgc atcagtaaaa agaatagtac agcatcctct tgacgcaatt
     1621 ctctttgacg caagtgtgta cgaacagttg tcaaatgttc ctgccacctg gtctcgctga
     1681 ttttatgtcg tgttagaaga gaaagtggca cagtttgtag aaagtggaga tattgtttcg
     1741 ttatattgac aaattgataa ttcatgatcc ctcgatggat tcgacggaat cttttacgta
     1801 cgatttggtt ggccagtttt ttacgtaatg aacgctcctt ttgttctgta tattcaagat
     1861 ttggatctgt tgcgtatagt tcttggagct gttcttccgc cagatctttg acccattgct
     1921 gacgtgtttc gtctttttgc agaccgccta attttttcag gagcttcgtt tgtaatagag
     1981 aaagtctatg atgtaaaggt aattgttcat ttgtttcttt ataccattgg cgaatcgatt
     2041 cctttgatag gatcgtacgg ccatttattt tcatatcacg gaacaaggga ccgtagtttg
     2101 tgatgccttg gatatagcga tcaatctgat cgactaaggt caatccgctt ttcatcactt
     2161 gtatcggatc tttttcccct agcaaaaaac cactttcttg ttgctgttca tctagtaaag
     2221 aaaactctgg cagcagctgc accaaataat ttttgaatgt ctgtgtaggc acgccgcttt
     2281 cacctaatga aggcaataca gtcgagatgt aatcagaaaa caaatggttg ggtgaaaata
     2341 gaagaacgtt ttcagcctcg agccacttgc gattatgata caacagaaaa gcaatacgtt
     2401 gaagcaaagc ggatgtcttt ccacttccgg cgattccttc aatcagcatt actttacttg
     2461 ttgtgtcccg aatgattgca ttttgtgctt tttgtatcgt agagacgatg tttttcatat
     2521 ggttgcttga tgattcatcc aatatctcta gcaaaaagtc atcattgatt acttccgacg
     2581 tatctaccat agataaaata cgtccttctt ggattttaaa ttgtcgtttc aactgcagat
     2641 cgacatctat tctatctgtt tcagtttcat aaaatgcggg accaatctca ccttcataat
     2701 aaagattggc aattggtgcg cgccagtcaa taacgattgt ttcttctgcc agatctctta
     2761 aagaagcgat acctagatat aatgtttctg tttcacggtc ttctttaaag tcgatacggg
     2821 caaaataagg actatctttc atgacagaca acgtttgcag gcgtttttgc tgtgcttcaa
     2881 gagtatggta tttcaaaagc aattcctgct catgttggcg atattcaaca gcagattcat
     2941 aaaaagcttc attggaacca gctcgaatct tgtgattagc tgtgtctttt gtcgaatgat
     3001 tcatttcctg agtcaattct tgttgttttg tatccagcat attttgttcg atctggatta
     3061 gttcgatcgt ttcatccacg tgcgtttgtt ctagttgttt ctcattcatc gcatgtttgc
     3121 tccttccgcc gtaaataacg aattaaaagt atagcatagt ttttcgaaaa aaggtacttt
     3181 ttaactgatg aatcgaaaaa gtaatccttg ctgaatattt ttgtttgtag aactgatgaa
     3241 ttttggaaac gctgatagtg atagttgttt tcaattaagg cctattgttt taaaaagtgc
     3301 tagtcaagga ctatgcttaa gatgaaagag gcagctggaa atttattact aacctctcta
     3361 agtcacataa aagaggagaa aaaacaatga cacatttcaa aaaattttta cttactttgc
     3421 ttattggtgg gatagcttta ttaagtgaat ttgtttttca ccagccggcc cttgcatttg
     3481 cgattgtcgc atttactgga gggattctcg ccttcttgat gtttttggaa atgatcaaga
     3541 cgttacgttc aggaagatat ggcgtagatg tattagcgat taccgcaatc cttgcaacat
     3601 tagccgtcaa agaatactgg gctagtttga tgatattgat catgcttacg ggcggagata
     3661 gtctagagga ttacgcgaat aaacgagcgg gtaaagaact gcagtctttg ttagataaca
     3721 ccccaagaat tgcccatcga ttcaaagaag gaaaattgca ggatatagta atcgatgaaa
     3781 taaatattga tgaccagtta ctagtcaagc ctggcgaact tgtacccgca gatggtcgag
     3841 tgaccaaagg agaatcttcg gtcgatgaat cttctttgac aggtgagtcc cgcccggaaa
     3901 ccaagaaacc tggagatcag attatgtcag ggtctttaaa tggagacgga tcgatccaaa
     3961 tgatcgctga aaaacgtgcc gcagacagcc agtatcaaat gattattaaa ctagtcgaag
     4021 aatcaaaaga aaagcctgcc cgatttgtcc gtttagctga tcgttatgca gtccctttca
     4081 cgttgattgc ttatttgatc ggtggtattt cttggtggtt ctcaaaagac cctttacgat
     4141 ttgctgaggt actggtagtg gcatcacctt gtccactgat tctagctgcg cccattgctt
     4201 tagttgcagg aatgagccgt tcaagtaaaa acgggatcgt tgtaaaaact ggaacaacga
     4261 ttgaaaagct cgcacgtaca aaaacgattg cttttgataa aacaggaacg cttaccaaag
     4321 ggatattgga agtagaggcg attcagccag tcaatggaat aaaaaaagaa gagctacttc
     4381 tgtatgcagc tagtgcagaa caggaatcta gccatatctt agctcgttcg ttagtgaaca
     4441 acgtgaaaaa cagccaactg tatcctgtta gtcaactgaa agaagtgacc ggtcaaggaa
     4501 tccaagcagt agtcaataat catgaagtaa agattggacg tgcctctttt gtttcagctg
     4561 aagaaataga aacgtccaat acagcaatct atgtaacaat tgatcgatct tttgcaggaa
     4621 cgatcctatt ttcggatcag attcgcccag aagcagcaaa gacaatcaag gagctaagaa
     4681 aacttgatgt gaaaaatttg atgatgatca ccggggatgg atctgccatt gctcaaacga
     4741 ttgcagaaaa agtccaatta gattctgtac atgcccgtag cttgcctcaa gataaactga
     4801 ccattttaga aagtatccct aaagatcaaa gacctgttgc gatggtagga gacggcgtga
     4861 atgacgcacc agcacttgct gctgcagata tcggtattgc aatgggggct catggttcta
     4921 cagctgctag tgaaagtgca gatgcggtca tcttgaaaga tgatctcttg cgtgtagcaa
     4981 aagcagtagt gatttcaaga gatacgatga gaatagcgaa acagtctgta ctgattggca
     5041 tctttatttg tatttttttg atgttaattg caagtacggg actaatcccc gcattattcg
     5101 gagctgtttt gcaagaagtc gtggatacgg tctctatttt gagtgccttg agagcaaaaa
     5161 aagatccagc catttttcct gattcagtac ccaagacaaa catagtatga tagaatcgag
     5221 ttgaaaaaag aacgatcaga aagaggagag agcattatgc cattcgtaca tgtcgaatta
     5281 gtagaaggtc gcagtccgga acaattagaa aatatgatga aagatatcac ggaagctgtt
     5341 cataaaaaca cgcaggcacc aaaagaacac atccatgtca tcatcaatga gatgaaaaaa
     5401 ggaacatacg gtgtaaatgg ggaatggaaa aaataagttt agtttcccac ttgatttttc
     5461 tatttattag tggttaaata aacacataat tgattgatga gaaagccaac tgaatttgag
     5521 agtcttcgat aagagaggaa aagctaggct gaaacttttc caggagaaaa ccaaaggatg
     5581 accactttcg aaacccttgt tgaagcaagg gcggtcggac cgttacaacg cttgaggaac
     5641 gcatttgcgt ttgaacatta ggtggaacca cgaacttttc gtcctagtat ctcttttcaa
     5701 gagatgctag gatttttttg tttttcagct tgatgatctg atcaatcaaa acatttcaag
     5761 gaggaaacta ttatgataaa aattactttt ccagatggtg cagtcaaaga atttgagtct
     5821 ggaacaacaa cattggcaat tgctgaaagc atttcaaaaa gcttagcaaa aaaagcttta
     5881 gctggtaaag tgaacggaaa actcgttgat ttgactcgtc caatcgagga agatgcaagt
     5941 attgagatca tcacaccaga ccatgaagat gcacttggct tagtccgtca ttcagcagct
     6001 catttgatgg cccaagccat gcgtcgcctc tatccaaata tccactttgg cgttggacca
     6061 gctatcgatt cagggtttta ctacgataca gataatggac aaaaccaagt aactgcagaa
     6121 gatttgccgg caatcgaagc agaaatgatg aaaatcgtca aagaaaatct gccgatcgaa
     6181 cgccgtgttc tttcaaaaca ggaagcatta gaaatttttg caagtgatcc gtacaaagta
     6241 gaactgatta gtgaattgcc agaagaagaa gtgattactg cttatcaaca aggcgaattc
     6301 atcgatttgt gtcgtggacc acatgttcca tccactggcc gtatacaagt atttaaatta
     6361 ttatcagtgg caggtgctta ttggcgtgga aactcaaaca atcaaatgat gcaacgtgta
     6421 tacgggacag ccttttttga caagaaagct ttgaaagaat atatccgtct ccgtgaagaa
     6481 gcaaaagaaa gagaccatcg taaactagga aaagaacttg acttgttcat ggtttcccca
     6541 gaagtaggtt ctggtttgcc attctggctt ccaaaagggg caacaatccg acgcacgatc
     6601 gaacgttata tcgtagacaa agaagttagt ctaggttacc aacatgtata tacaccaata
     6661 atgggagatg ttgagttgta caaaacatct ggacactggg atcattatca agaagacatg
     6721 ttcccgccaa tggatatggg agatggcgaa atgcttgttc ttcgcccaat gaactgccct
     6781 catcacatga tggtctacaa aaacacgatc cattcttatc gtgaattacc aattcgtatc
     6841 gctgaattag gaatgatgca tcgctatgaa aaatcaggtg ctttgtcagg tttacaacgt
     6901 gtacgagaaa tgacgttgaa tgatggtcat acatttgttc gtcctgatca aatcaaagat
     6961 gaatttaaac gtacgcttga attgatggtg gcagtatatg ctgacttcaa tattacagat
     7021 tatcgtttcc gcttgagcta tcgcgatcca aacaatacag ataaatattt tgatgatgac
     7081 gcaatgtggg aaaaagccca agcaatgctg aaagccgcta tggatgaatt agaactagat
     7141 tactttgaag cagaaggcga ggcagccttt tacggaccaa aactagatgt tcaagtcaaa
     7201 acagctcttg gtacagaaga aacattatca actatacaat tagacttctt attgccagag
     7261 cgctttgatt tgacatatgt tggagaagac ggggaaaata cgcatcgtcc agtagttatc
     7321 caccgcggaa ttgtttctac aatggaacgt tttgtcgctt atttaacaga agtatacaaa
     7381 ggcgcttttc caacatggtt ggcacctata caagcaacaa tcatccctgt ttctgttgat
     7441 gcacatggcg attatgctta tgaaatcaaa gaacgtttgc aaatgaaggg gttgcgtgta
     7501 gaagttgatg atcgtaatga aaaaatgggc tataagatac gtgcctctca aacacaaaag
     7561 atcccttacc aattagttgt tggagacaag gaattggaag atgcaacagt gaacgtacgt
     7621 cgttatggaa gtaaagaaac agctgtagaa gatttgaata tctttattga tgctatggaa
     7681 gcagaagtca aaaattacag cagagaaaac taataggtaa atttcctgta aaaaagtagt
     7741 agatagaaag caatccgaac aatagataac aggtattcct caaagacaca gatgctcaat
     7801 agaatctgtg ggattgatgg gataagatat tctgttgggc ggattgtttt tttgtttttt
     7861 atccagtata cgaccgtgaa taagggtctg atatggtata cttacataga ctatcattaa
     7921 aagggtggag gaacttatga gcgtaattag tcgtattgaa agtatgatgg aagattattc
     7981 ttcagcagaa aaaaagttgg ccaactacat tatcggtaat attgagaagg tccctacaat
     8041 gacagctaat gaattagcag aagcttccgg gttgagtgcg ccgacagttg tacgtttttc
     8101 caaaaaaatc ggttttcaaa gtctgacgga cttcaaaatc acgatttcaa cagaactaca
     8161 gactggaatc gatgaaggtt ttagtgatat agaagcaaat gagtcctttt actctatcaa
     8221 aaacaaactt ggtaacaatg cacaagtagc gataaaagaa acagtagata ttctagaaga
     8281 agaaacgatc caaaaagtcg tagagtgttt ggaatcggca gagactgttt ttttatatgg
     8341 ggttggcgcc tcttctttag tagtagaaga tattctgcaa aaatggtctc gtgtcggtaa
     8401 gccaatcatt tttgaaaaag atatccacgt cttgctcccg caacttgtca gcaatgagaa
     8461 aaagaaagtg ctttggcttg tttcaaactc aggtagaagt gcagatgtag tcgcattagc
     8521 cgagctggcg aaaagcatga atatcgaaat cattgcgttg acgcaatttg gaaataaccc
     8581 gcttagcaag atagccgatg tactcgtaca aacatctaga ccaaaagaaa tcacgaatcg
     8641 aagtgcggca actaattcac ttttggccca atttgctacg atcgatatca ttttttattt
     8701 ctatatggct aaaaacgaaa agcttagcga aaaagtgaaa aaaacacgag aagcaatcca
     8761 agaatatttt atggattcga aataa
//
LOCUS       DASOUD010000092         8769 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.093, whole genome shotgun sequence.
ACCESSION   DASOUD010000092 DASOUD010000000
VERSION     DASOUD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.093"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(310..807)
                     /locus_tag="U4A29_002145"
     CDS             complement(310..807)
                     /locus_tag="U4A29_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO0681259.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="U4A29_002146"
     CDS             complement(847..1656)
                     /locus_tag="U4A29_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO0681260.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="U4A29_002147"
     CDS             1990..2847
                     /locus_tag="U4A29_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO0681261.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="U4A29_002148"
     CDS             2840..3742
                     /locus_tag="U4A29_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO0681262.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="U4A29_002149"
     CDS             3775..4125
                     /locus_tag="U4A29_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEO0681263.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4174..5097
                     /locus_tag="U4A29_002150"
     CDS             4174..5097
                     /locus_tag="U4A29_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO0681264.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="U4A29_002151"
     CDS             5175..6068
                     /locus_tag="U4A29_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO0681265.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="U4A29_002152"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="U4A29_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO0681266.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="U4A29_002153"
     CDS             6683..6871
                     /locus_tag="U4A29_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO0681267.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="U4A29_002154"
     CDS             6884..7786
                     /locus_tag="U4A29_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO0681268.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="U4A29_002155"
     CDS             7818..8060
                     /locus_tag="U4A29_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO0681269.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8316..8687)
                     /locus_tag="U4A29_002156"
     CDS             complement(8316..8687)
                     /locus_tag="U4A29_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681270.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3053 a         1519 c         1704 g         2493 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaaga tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
     8161 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
     8221 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
     8281 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
     8341 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
     8401 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
     8461 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
     8521 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
     8581 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
     8641 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
     8701 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
     8761 cgtaacaaa
//
LOCUS       DASOUD010000093         8766 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.094, whole genome shotgun sequence.
ACCESSION   DASOUD010000093 DASOUD010000000
VERSION     DASOUD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.094"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(295..1248)
                     /gene="menA"
                     /locus_tag="U4A29_002157"
     CDS             complement(295..1248)
                     /gene="menA"
                     /locus_tag="U4A29_002157"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEO0681271.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1261..2364)
                     /locus_tag="U4A29_002158"
     CDS             complement(1261..2364)
                     /locus_tag="U4A29_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO0681272.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2454..3410)
                     /locus_tag="U4A29_002159"
     CDS             complement(2454..3410)
                     /locus_tag="U4A29_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEO0681273.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3691..5625
                     /locus_tag="U4A29_002160"
     CDS             3691..5625
                     /locus_tag="U4A29_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO0681274.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            6082..6498
                     /locus_tag="U4A29_002161"
     CDS             6082..6498
                     /locus_tag="U4A29_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEO0681275.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6512..7078
                     /locus_tag="U4A29_002162"
     CDS             6512..7078
                     /locus_tag="U4A29_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEO0681276.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            7101..8087
                     /locus_tag="U4A29_002163"
     CDS             7101..8087
                     /locus_tag="U4A29_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO0681277.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8242..>8766)
                     /gene="scm"
                     /locus_tag="U4A29_002164"
     CDS             complement(8242..>8766)
                     /gene="scm"
                     /locus_tag="U4A29_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEO0681278.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2581 a         1694 c         1770 g         2721 t
ORIGIN      
        1 ttttcttaat actcgaaact aagcgacttt ttcacaacct ctgataaacg gtgttcaaaa
       61 gccagtttct cggtattaag ccaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
      121 tttttcttaa tactcgaaac taagcgactt tttcacaacc tcctgataaa cggtgttcaa
      181 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tatttttatt
      241 tggctgttct tattacttga tgcagggcag ttttttcaca acctcttcat gctgttaaat
      301 ggcttgcata atgattgtta gtaatagccc caatacataa ctggcattga ataagaccat
      361 atttttgatg gaatagctga aactgatagg ctggggtaac gtttgcttga attgatccaa
      421 atgcttcttc acaattggca aggtcaagaa ggtgaccaag atcggccatt catagatacc
      481 gctcaacaac cccattaaaa tcacgaaata gcaagcatac atcaatagct ggaataagac
      541 tgttcccgca ggacgtccga tataaaaaac tagtgtatag cgatgatttt caatatcacg
      601 gtcaagatcc cgcaaattat tagccaacat gatattggca atcgtaaaca ccaagggtaa
      661 ggaagctaat acgattgctg ctatagccca aagatttcct accaaatcaa attgtccaga
      721 tgaaaagttg actgtcagat catagacatc tgaaccgaat gtattgacat aaacgaccat
      781 ggcaaaaatt ccaaggccca tagtcacccc actgaaaatt tcccctaaag gcattcgaga
      841 taaaggaatc ggtccgaatg tatagaatac tccaataaaa caacagataa atcccatcaa
      901 aagtaaaagc caacccgtat tgatcgttag aataacgccg ataacaaaaa caaatgcaat
      961 catagctaaa atcatgtttc gaatcaatac aggagataga tttctgcggc caatgacatt
     1021 ttcttcatac ttatatactt cagaatgagc cttttcataa tccatgtaat tattgatagc
     1081 ggtagtcgct aaatcaaaaa ctagcattcc aataaaaaat aaaaaagtgt ttagccaatg
     1141 tatctcatga aaaaaagcga tagagaataa tacccctacc gcaaaaggaa atacactagc
     1201 cacctttgtt cgtatttcaa ctacttctaa aaagattttt agtgtcatgg ttcgtactcc
     1261 ttattttagt tcagaacggt cacccatcgt gtaatgggag tcctccgata atttgaaggt
     1321 gtctttgatt ggatcggtca catatacttt atcgtctttt gtcacgaaga tcgcttccgt
     1381 cccgttattc aattcgcttt cgacgtattc cagtcctttt ttcacaccca ttgaaaagac
     1441 tgctgtagac aacccgtcac catcaattga tttatctgtt atgatcgtga cacttgcgat
     1501 atcattgtcg aacggataac ccgtttttgg atcaaacaaa tgatggtatt ttttgccatc
     1561 tacttctaaa taacgctcgt aaattccaga ggtaactaat gttttgttcc gttctttaat
     1621 agaacctaaa acagatccgc gagccatgtt cggatcttgg atgccgactg tccagtcttg
     1681 gttttcgcct cttgggctgt ggcctaagac atagacatta ccgcccaagt caacgatggc
     1741 agtggtcact ccttgttttt tcaagacttt cactacttcg tccgtaatgt aacctttggc
     1801 gattgcacct aaatcgatga tcattccttt ttcttttaga taaacagttt tttctttgtc
     1861 atttagttcg atcttatgat aatccactaa tttcaatgcc tgatcgattt cttcttggga
     1921 aggttttctg gcgtcatcaa aaccgattcg ccataattgt gtgattgcac caatcgccat
     1981 atcgaatccg ccttgagaat cttggctata ttcgtaggcc cgcttgacta acgtgtagac
     2041 gtcatcagaa acttttactg gtttgatccc agcttgctca ttcacttcat cgatctcaga
     2101 acctttttga ttgatcgtga ttttatcacc aagttctttt actcgatcga aggcaggttt
     2161 caatgctgat tctttgcctt catcatacac acggatacga acgtaagtcc ctaacaagaa
     2221 ctgttcatca gaatatggat ctttcaatat cttggtagct gctgaacttt cagtggttgt
     2281 tttatttgct gagttgctgc agccagttag caccataaaa gagatgccca gtgtgattaa
     2341 ggaaaatagt agttttttcc gcatcttttt cctctcttca ttttgaaagg tgagacgact
     2401 gcctaatctg gctatcgtcc caccctccaa cttttattag tatgattatg tgattatttt
     2461 tctgttacga tattatctac ttcgattgtt tgagtgtcgc ctttttcagc tgcattcact
     2521 aattgttgtg cgtacatgat gaatgaatgt gagctgtgtg tagcacctgt aacaacttct
     2581 acattagcag gtgagccatc ttcttcgccc attgctttga cgaattcgtc gttcagagta
     2641 gggatgtaag tttttggttc tgtaccgctt tttgctttca tattttcatt atattctgta
     2701 tcatcttgtt ttgatttacc gtctttgtcg atataatcaa agtttgattc agttactttg
     2761 ccgccagcaa ctgtcatttc gaattgcaca cggtagccgt tagagtagtt cttttcttca
     2821 agcttgtaag tgccgtcttt caaatcagca ccattgtcga tttcgattgt atctgtgtta
     2881 ccagcttgag cagcttggat caattgttgt gcgtagtttt ggaagctttc agatgagtga
     2941 gtagctccag ttacgacttc cacaccgctt gcgctttgtg cttttaagaa ttggtcattc
     3001 aactcaggaa tgtatttttc tgggtttgtg ccagattttg cttccatgtt tttgttgtat
     3061 tctgcatctt ttgtttttga ttcgccgttt tcattgacgt tatcatattt tgattcagtg
     3121 attttgccgt cttttacaac catttcaaag actgcacggt aaccatttga ataatttttt
     3181 tcttctaatt tatatgtgcc gtcttgcaaa tctccgccag ctacaacttt tgcagatgat
     3241 tctgttgtgc tttttgcagt cgaagattgt gctgtagatg attcagaaga agagtttgag
     3301 cttccgttgt catctgcgcc gcaagcgcct aacactagag ctgaaaatgc taatacggca
     3361 aatccagtta caaaacgttt tttcttcatt ttaatacccc accattccat ttctagtcca
     3421 caatttttct ttgtgattca ttatacattc taatcttttt agcctataag tcaactattt
     3481 ttgataaata acccatgtaa tatttcaaac gttttctttc agcaaatttc gtgaaaaaac
     3541 gctttatttt aaaatttgaa aaaaatccaa aaggagttag acaaaagaac agaacatcta
     3601 tataatatat ggagattgtg taataagtat caaactaaaa gaattatcat atattcatta
     3661 tatagatagt ctataaggag cggatgatca atggcaaagc aaaatattgt cgttgtagga
     3721 gcaggatatg ccggtgtttc agctacaaaa tacttggcaa agaaactgaa aaaagaagat
     3781 gtagcaatta ctctgatcga tcgtcattcg taccatacta tgatgaccga gttgcatgaa
     3841 gtagctggcg gacgtgtaga accagaagca atccaatacg atctgcagca tttgtttgct
     3901 cgtcaaaaga atgtacaact tgtcacagac actgtaatcg gtatcgataa agaacgaaaa
     3961 atcgtcaaga ccaaattagg ttcctatcct tttgatcagc tgatccttgg aatgggcgga
     4021 gagccgaatg actttggtac acctggtgtc aaagcacacg gattcaccct ttggtcattt
     4081 gaagattctg taaggattcg ggaacatatt gagaagacag tagcaaaagc tgcattggaa
     4141 ccagatgatg aactgcgtaa agccatgctg actttcgttg tctgtggttc tggatttacc
     4201 ggtattgaaa tgattgggga actgatcgac tggaaaaatc gcctagccaa agattataaa
     4261 ttagatccaa acgaaatcac actgatggtc gtagaggcaa tgccgacgat cttgaatatg
     4321 ttggatcgaa atgatgctgc aaaagctgaa cgctatttgc gtaagcacaa cgtggaatta
     4381 ttattgaatg caccaatcgt agaagtggct gctgatcata tcaaattgaa agacggacgc
     4441 aacattccga ctcacacact gatttggaca gctggtgtca aaggaacttc tgacgctgct
     4501 gactttggat tagaatcagc acgcggacag cggttgatcg ccaatgaata tatgcaggca
     4561 aaaggctacg aagataaaaa catctatatc attggtgatt tagtctttta tgaagaattc
     4621 ccaggaacac cgacaccgca gatcgttcaa gctgctgaac aaacaggaca cacagccgca
     4681 gcgaatatcg tggcttcgat caaaggcgga gaaaagcata aatttaaagg aaactatcaa
     4741 ggatttatgg tttctattgg tgccaaatgg ggggtagcta acctatttga taagatccat
     4801 ttaagcggat ttttagctat cgtaatgaaa catatcgtga atttgaaata cttctttgat
     4861 attcgttctg gttattatat gttccaatat ttaatgcatg agtttttcca tatcaaagat
     4921 gatcgcaacg tgactcgagg acattcttct cgttacggca atgtcttatg gagtgtgcca
     4981 cttcgtattt tctatggttt agtttggttc gttgaagctt ccaaaaaaat cattgggaat
     5041 ggtgaagtgt tcaaaccaag tacttggttt ggtgaaggat catggttcac tggaaacagt
     5101 gtgttccctt ggacatggga atacaatgta acagatgttg ccagtggtgc ttctgctgct
     5161 ggtgatgcaa ccacagctgc cagcggagca ggggcaagtg ccggagaagc agcgactcaa
     5221 gctgcacatt ttggtttgag ttatgcatac ggtgaagaac caatggctgt gattgagaag
     5281 tcaccagact ggttcacaaa gatcatgcaa gtgatcatgc caaacaatga tgtagcaatg
     5341 ttcttccaaa aattcatggt attctttgaa attgccttgg cattagcatt gattgctgga
     5401 ttattcacat ggctatgcag tgcgacaacg attgtattgg tgatcacgtt ctgcttatca
     5461 ggcatgtttt actgggtaaa catttggttc atttttgttg ctttcgcatt gatgaacggt
     5521 tccggacgtg cgattgggtt ggatcgctgg gtaattccat ggctacaacg tacactaggt
     5581 aattggtggt acggtagacc aagttcccag tacggcaaga aataaataga taaagaacgt
     5641 tatttgaatg caagatttca atagaaaggg gctgagacaa accgtcacag cctccttttt
     5701 atgtaaaatt aaaggaataa gatgcccgag ataagcctcc atgagcaaga cacaggtgtc
     5761 acaacctctg ttacaacctc ttgttataaa cggtgttcca gaagtagctt cttcgaaaaa
     5821 taagccgaaa tttcacaaaa acttgaaaag taattttcgt aaattccttc ttatttcttg
     5881 aagttgccca cttctgtcac aacctcttgt tataaacggt gttccacctg ctgcttctcg
     5941 gtaataagcc caaagaatta gaaaacatgg aaagctgttt tctattcttc gttcttatta
     6001 ctcaaagcaa gacacaggtg tcacaacctc ttgtttttat gctaaaattg ggtgagttaa
     6061 tctggaaaaa ggagaacaaa aatgagcctt aaagaatttg ctgaaaaaag tctgctcaaa
     6121 ccttgggatg tttttattat tttgcttttg actgttcttt cttttttacc agtcatgatt
     6181 tttggtcttc agcaaaacac cgcaacagca gaaaaagaag cggtactgcg tgtggatggc
     6241 acagaaatca aaacattccc tttgatggct ggagaagaga gcaacacata tacttacaaa
     6301 gatgaacacg gagattataa cttgatcgag atagatggcg atcgagtacg gataaaagaa
     6361 gcagattgcg atgaccagat ctgcgtacgt cgaggctggg caacgaaaaa tggcgaaacc
     6421 atcgtctgtt tgccacataa attagtgatc gaagtccgtt caacagatgg aggtgacgaa
     6481 gacagtctga tttattagac cttcacacac catgagtaaa ttacaaaaaa tgatttatat
     6541 ttcgctactt gttgcacaag gagtgatcat tggtttattg gaaaatatga tcccttatcc
     6601 tttttctttt gcgcctggtg caaaactagg cttagccaat ctaatcacga tcgttgccat
     6661 ttttaccatg aaaaaacaag acagcttctt actactttgg ctacgactat tcctgactac
     6721 tttgctaggc ggaacgattt ccacattcct ttatagtatg agtggtgctt tgcttagtta
     6781 ttttggaatg ttattggtca agcaattagg accaaaacgt gtcagtatca tcggaattag
     6841 tgcggccggc ggtttcatgc ataatgtcgg acagcttttg atggcttctt tcattgcaca
     6901 atcatggaca gtcatgttgt acttgccagt cctttcagtt ttagggattt tatcagggat
     6961 tgcaatcggg atcgcagcga attatctttt acgacatgtg cagacacttc aacgattcca
     7021 aaaagattat gagcagggaa ccaaacaaaa atggagccca atgtttacca aaaaataatc
     7081 gtcacacaag gagttttttt atgaagctac atccaatgtg ggaaaactat cctgctttaa
     7141 gtaaagaatt gacagaaaca ctaaaacgga tggaagaaac tgtccgcctg aaaaacaaac
     7201 ctgtcgaaca agccatcaaa gaaacgatcc atgccggcgg aaagctgttg cgtcctgcct
     7261 atcagctatt gttctcacaa tttggaccag agcaagatcg agacaaagcc atcgctttgg
     7321 cagcttctat cgaaatgctc catacggcca cactgatcca tgatgatatc gttgatgaag
     7381 cggatctgcg acgtggtcag cctaatatcc gtagtcagtt tggtaatgtc gtttctgtct
     7441 atgcaggaga ctatttattt gtctgctgtt ttaaactatt gtctgattac tccacttcct
     7501 tgaaaagtct ccaattgaat tctcgcagta tggaaaaagt attgaatggc gaattagggc
     7561 agatggatga tcgctataat tatgaacttt ctgtaaaaga atatttggaa aatatttcag
     7621 gtaagacagc tgaactgttc caattaagtt gttctgttgg cgcttttgaa agtggaacaa
     7681 gcgagcgctt cgcaaaaaaa gcaggagata tcggactttc tatcgggatg gcctttcaga
     7741 ttatcgatga tattttagat tatacaaaag agagccagga aatcggcaaa cctgtactag
     7801 aagacatgcg tcaaggggtc tattctttac cgttgatcta ctctttgcaa aaaaacaaac
     7861 caaagctact accttattta gagaagaaag cggcaatgac agaagaagat gtcgacgccg
     7921 tacgcaaaat cgttgaacat acaggaggcg tggaagaagc taggaagtta gcggcctctt
     7981 acacagaaaa ggctttgaag gaaataaaga aattacctgc cacctctctt cgaacaaaag
     8041 agaatctctt ttcccttacc caactgattt tggatcgcaa agactgaata gggaaaggtt
     8101 cgatacgtaa ataaatttct cagaaggaaa tctacgtgtt ttttgacaaa ttgaattgta
     8161 gttcttgtat ttatgataaa aagcaaaaat aaagagcaac gtccgttagc gacgttgctc
     8221 ttttttatct tgaatatttt tttacctttt cgatttctta taactcataa aagtgattct
     8281 taaaccgatt acaagtaata taacgcctgc gagtgttatc caattattcg attgacttcc
     8341 agttttagga agtgctcttt ttgatttgtt tccattgtca ttgcttgttt tattagttaa
     8401 gttaaagttc gtcctgttat ccgaacttgt ttttgagaag gagacacttg catgacgtgt
     8461 atcgcttgtg gtagacgacg tgtctgtact atcgcttgaa gttgatgaat ctgagctatc
     8521 acttgtggta gaggatgtat ctgtactgtc acttgaagct gacgtgtccg tgctatcact
     8581 tgtagtggta gatgaatctg agctatcact tgtggtagag gtagtatctg tactgtcact
     8641 tgaagctgac gtgtccgtgc tatcacttgt tgtggtagat gtatccgtac tgtcgcttga
     8701 agctgacgtg tccgtgctat cacttgtggt agaggtagta tctgtactgt cacttgaagc
     8761 tgatgt
//
LOCUS       DASOUD010000094         8592 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.095, whole genome shotgun sequence.
ACCESSION   DASOUD010000094 DASOUD010000000
VERSION     DASOUD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.095"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(51..1517)
                     /locus_tag="U4A29_002165"
     CDS             complement(51..1517)
                     /locus_tag="U4A29_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEO0681279.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="U4A29_002166"
     CDS             1904..2500
                     /locus_tag="U4A29_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO0681280.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="U4A29_002167"
     CDS             complement(2558..3040)
                     /locus_tag="U4A29_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681281.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="U4A29_002168"
     CDS             complement(3146..3343)
                     /locus_tag="U4A29_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681282.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="U4A29_002169"
     CDS             3638..4396
                     /locus_tag="U4A29_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEO0681283.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="U4A29_002170"
     CDS             4456..4884
                     /locus_tag="U4A29_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681284.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="U4A29_002171"
     tRNA            4930..5002
                     /locus_tag="U4A29_002171"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="U4A29_002172"
     CDS             complement(5152..5523)
                     /locus_tag="U4A29_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEO0681285.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="U4A29_002173"
     CDS             5738..5923
                     /locus_tag="U4A29_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681286.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="U4A29_002174"
     CDS             complement(6006..6194)
                     /locus_tag="U4A29_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681287.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="U4A29_002175"
     CDS             complement(6218..6481)
                     /locus_tag="U4A29_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681288.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="U4A29_002176"
     CDS             6639..6896
                     /locus_tag="U4A29_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEO0681289.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="U4A29_002177"
     CDS             complement(6967..7158)
                     /locus_tag="U4A29_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681290.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="U4A29_002178"
     CDS             complement(7299..7619)
                     /locus_tag="U4A29_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681291.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="U4A29_002179"
     CDS             complement(7642..8076)
                     /locus_tag="U4A29_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO0681292.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..>8592
                     /locus_tag="U4A29_002180"
     CDS             8411..>8592
                     /locus_tag="U4A29_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO0681293.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2723 a         1695 c         1362 g         2812 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tc
//
LOCUS       DASOUD010000095         8519 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.096, whole genome shotgun sequence.
ACCESSION   DASOUD010000095 DASOUD010000000
VERSION     DASOUD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.096"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1467..2654
                     /locus_tag="U4A29_002181"
     CDS             1467..2654
                     /locus_tag="U4A29_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681294.1"
                     /translation="MKNYNRISANNQLPMIESLRVPTNADKKIYGHVVVPEGVEAKPR
                     DAWTGEVEVTLRITYADTTKSSVEITALTQGQTDENEGAGYNPWGEGEQGGLFEVQVP
                     NDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVDVIPPEPANLSTTLINAASRTIT
                     GTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPENQLFAGDVLEIVLNDQAGEATAK
                     GVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKIVVEGGLSFLAPSKLDFGQIKAT
                     GLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQPFTNDAGFSMQDVLYFKKGEEY
                     QLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLVLTKENQIAGNYSAEITWTLADA
                     PTE"
     gene            2674..3219
                     /locus_tag="U4A29_002182"
     CDS             2674..3219
                     /locus_tag="U4A29_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HEO0681295.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            3247..3702
                     /locus_tag="U4A29_002183"
     CDS             3247..3702
                     /locus_tag="U4A29_002183"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HEO0681296.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            3699..4457
                     /locus_tag="U4A29_002184"
     CDS             3699..4457
                     /locus_tag="U4A29_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEO0681297.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVNPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(4543..5283)
                     /locus_tag="U4A29_002185"
     CDS             complement(4543..5283)
                     /locus_tag="U4A29_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0681298.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            5344..6597
                     /locus_tag="U4A29_002186"
     CDS             5344..6597
                     /locus_tag="U4A29_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO0681299.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            6919..7146
                     /locus_tag="U4A29_002187"
     CDS             6919..7146
                     /locus_tag="U4A29_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEO0681300.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            7269..7595
                     /locus_tag="U4A29_002188"
     CDS             7269..7595
                     /locus_tag="U4A29_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEO0681301.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            7663..8376
                     /locus_tag="U4A29_002189"
     CDS             7663..8376
                     /locus_tag="U4A29_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO0681302.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         2918 a         1456 c         1785 g         2360 t
ORIGIN      
        1 gaacagctgc acatccaggt ggattgacac caaatatcga tggaaatgcc attatcattg
       61 cttatatgac cgatttttgg ggaagcagat atcattttac gggagaaata aaatttaccg
      121 ggacacttga tttgtatgat gggtccaaat taggactgat ttacgctcct cgtgcaactg
      181 tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc agtgaaggaa ggactgaatg
      241 ccgctccggg taaagcctat ttttgtagaa gctatcaatt gaatgtgatc aatggtagtc
      301 aactttatgg accgtatcta gggaaattct acggttttct agattcagga gatgagactg
      361 gtagtggaaa aacagcgcca ggaatccata tcttaagcgg ttcaaaggtc tctcttgatt
      421 atgatcgggc agatggcgtt agcgatgaag gagaggcagt cgatacgcta cctggtaatg
      481 tcatgtttga agtcagtggt tcgggaagcg aattttcggt aaatacaaag atcaatatta
      541 ccaatgacaa caatcgcgga atcatccaaa tgagaggttc aggatcgaaa gtcactgtat
      601 cgaatggtgg gaaaatcacc atcaatacag caactacggg cggatttcgc ttgcaaggag
      661 attctagtca aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg
      721 acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa aggcctttct ttgatcgtag
      781 aaggagccgg atcagttatc gacatcgata agcagagtgg gcgttccagc gcagttcgtt
      841 ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg ctcattgagg gtaagaaata
      901 caggtgatgg caaatcttac gtgaacagac tcaatcaagg aaatcaagcg atccaatttt
      961 atgacgcttc tggttttcta gaaagtccag gttcagctga ttttacagtc actggagaaa
     1021 aatcaaatat cgatatacgc gcagattccg gagcaactgt agatacaact ggagatattg
     1081 atttaaattt tacggcaggt gaaaaaactt atttagtcat ggtcgggaaa acaggggaag
     1141 ataagaccgg catcatcagt ggggatgtgt tgaatgtgga cttagaatct cctacctatt
     1201 ttgatttcca aaacaaacgg attggaagcg agaccaatgg cataggcggc tgggtattcc
     1261 aaggaaatgg agattcttct ttaaagatcg cccattcaga aatcgcttta tggcgaaaaa
     1321 caggcgcaac attcgataac tttgaaaaag acccggatta tttttctgat atgacagact
     1381 tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag ccctagtgca ggtttaacaa
     1441 atgaatttgc tcagacaggt gccggaatga aaaattataa tcgaatcagt gccaataatc
     1501 agctcccgat gattgaatcc ttaagagtgc caacgaatgc agataaaaaa atctatgggc
     1561 atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag
     1621 aagtgacttt acgtattact tacgcagata cgacgaaatc atctgtagaa attactgctc
     1681 tcacgcaagg acaaacagat gaaaatgaag gggcaggata taacccatgg ggagaaggcg
     1741 aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga gagattaaaa acggaggatc
     1801 aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca agaagtgaca gaactggact
     1861 ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc taatttaagt accacattga
     1921 tcaatgcagc ttctcgcacg atcacaggta caggtgaagc aggagccttg gtttcattga
     1981 aaaaaaatca agctgttttg tcagaaacaa ctattgatga tacaggggaa tttgttcttt
     2041 ctatcccaga aaatcaactt tttgcaggag atgtattaga aattgtatta aacgaccaag
     2101 caggagaagc tactgctaag ggtgtgcaga acaagccaag taccaacgat gaaattggga
     2161 atcacaatcc aagtgatacg ccagttgttt atcatgatgc cccgcctttt gcacctgcta
     2221 cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc ttccaaactc gattttggtc
     2281 agatcaaagc aactggtttg acacaagaag catttggcca aatggatata caggaaaaat
     2341 tatggattta tgatcagcga gaaacgaaaa atccgtggat cttaacgata aaattatctc
     2401 agcctttcac aaatgatgca ggattttcta tgcaggatgt cctatatttc aagaaaggag
     2461 aggagtatca attagtgaat gaagaagaac tggaaattgt cagaggacaa aattcaacga
     2521 atgtgcaagc agattattca gaatatttaa ataacggtcg ggcgtttaaa cttgtcttaa
     2581 ccaaagaaaa tcagatagct ggtaattact cagcagagat aacatggaca ttagcagatg
     2641 cgccgacaga ataaagatca aggaggatga ttgatgaatc gaatatggaa taaaagtcat
     2701 cgaaacaact gctctttact tatgatcctt ctccttggat tcatcctttg gcagacacct
     2761 gtccatgcct ccgaattcaa ttttgcggta gattttgaat taccggctaa tcagattgat
     2821 aagaacaaaa catatataaa tgtaaggatg gagcctggga aaacacagga aatcccctac
     2881 acggtcttta accgaacaga taaatcagta acagttgaga cattgatcca ctccgctacg
     2941 acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa gtaaatttga tgaaacatta
     3001 agttatgata tgaaaaactt agcaaaaaca gtgggagaaa taactgttcc tcctcaaagt
     3061 gcggtcactg agacactttc actgaaagcg ccagaaaaag agctggctgg tgtggtcgca
     3121 ggagggatca cgtttcaaga aaaaaaagag aacgtttcta acaaaagtac agttggtgtc
     3181 gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa atagcgaaaa agagtcttcc
     3241 ccagatttga aattaaaaaa agcttatgcg gatcaaagcg atggaagaaa tgtgattaag
     3301 gcgaatcttc aaaatccaaa tgcttcttat atgttcggct tgactacatc ggttgaaatc
     3361 tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa aagaaaaaat agatgtagca
     3421 cccaatacaa attttgattt acttgtccca ttagaaggaa aaaaattagc tccgggacat
     3481 tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct ggaatctcca taccgatttt
     3541 gaaataactg atgatgcagc ggaccaattg aatgaaaagg atattgattt aaaaaatcag
     3601 ccagaaacat ttgactggcg atggctgata cttgttggag tcattcttat tggcttattt
     3661 gctggattat ttattttaaa agaaagaagg aaaaagaaat gaaaaaaaca attgcattaa
     3721 gtaccgttct agctggaagt ctattattat gtacacaaag tgtttttgct gagaataaga
     3781 gctcagccac tacagaagga acgatttctg tgaccaaacc agatgctgca aatcctgaag
     3841 acgttgttcc agttcctcct gttaatccaa atgatcctga tactcctttt acacctgacg
     3901 aaccaaatca gggaacatcc ggattactga cgattgacca agctccagat cttgattttg
     3961 gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc attacaaagc ggaacggaca
     4021 gtactggagc acaaacacaa gtacagaatt tcgtacaagt aacggataaa agcggaaatt
     4081 atgccggttg gaaattatca gtcaaacgaa cagaatttat tggtacaaca tctgatacga
     4141 ataaactaac tggttcaaag gtgaaattca aaaacgccgt gattcgaaat agcgcaacta
     4201 atagcagtgg aatgccatct accgtttctg gaactggcgc tgaaggaatc gaaatcccag
     4261 cagatcaggc agtagatcta gtaactgcag gagcaaatga agggattggc acatggattt
     4321 attcattagg agagaatgtg gaacaaggga gacaatcagt ggaattattt gttcctgtat
     4381 ccaattatgc tgtagacaac tacgtttcaa cattcacatg gtcgttaaca gatgctccta
     4441 cagaagagga agcgtaaaat catatcaacg atttcaggga gtggagccga ttcaaggccc
     4501 actccctgaa atctgctatg cttacgtctc agctcttatt catcaactcg aagcagtttt
     4561 ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc tttcgaccaa cgagtaagga
     4621 gtcaccgttg aaaaattcta acacataatg agcagaatca attttctgca attgcgataa
     4681 gttgatacag taagatttta aaagtaaaaa gtaagcatac ttttctaact ctgtcttcaa
     4741 ttgttttaaa gaagtattca ctaatttatc ttctgtagtt gttttgacca taacttggtt
     4801 tttcaaacct ttgatacttt caatatataa aatttcccga ggatacagga cgagacttcc
     4861 acctttttga tcaataaaga ttttatcgac atttacgatc tgctgttctt tccaattgat
     4921 ctgttctagc acttgacgaa tttgttcaga taaattcctg tcttttctga tatatccaat
     4981 cggaaggatt ttccgattga tgatctctac tgcaaaattc gtataagcgg tgaaaaaaac
     5041 aatattacta tgtgcatttt ttttacgtat ttgttcggcg acatcaatac ctgtgagtga
     5101 ttgcttcaag tcgatatcga taaagaaaac atcgtgatta tcaatagtgc ccaactggat
     5161 caactgattg atttgagaga tagaagtgag ctgcacgata ttctcgatat gatgattgac
     5221 caattctctg atgatccatt cgctgtgaaa ctggttatct tctatgataa aatacttttt
     5281 catatgcagg ctcctattaa aaaaatatgt gtacattttg aaacaaaaca atggaggaat
     5341 aagatggtcg caacagtgac actttatttg acctttactt tgattttaga tttaggtatc
     5401 tatttattct tttcgaaaca actgctagta aaaaacagct ttggttgtat agctttgatg
     5461 gtggtgacac ttttacttcc tatccctaaa gtgagctttc cttacctagt tggcttatta
     5521 ttgatgtttc ttttttattt tactacgaaa aacctgttta tcagctgttt gatcagtaat
     5581 cttttttttg ctatcactgg actcagcttt tttctggttt ttgatctgat ggattattta
     5641 ggaaagagac atcttggaac agcaagtttt tttgcactac ttatcgcatt tattttctat
     5701 ctattagtaa aaagtataga caaacagacg agatttctca acttgctttt tacttatcca
     5761 actgaacact attttggagg tatttgtatt ttattaagct ggggcgtgat ttacttggta
     5821 ttgggaagtg ttcacttccg ctcatctaat tatctgcttc tatctctttt gtcgatagtc
     5881 atcagttttt tttctttgtt tttttccatt atgttgatca gtttccaagt aaaagaaaga
     5941 caacatcagg aatcgcttca actgtatcaa ataaatgaag aatattacca tcatctagag
     6001 gaattcaaac atgattataa aagtctactg ttttcattaa aaattactct tcaggaaaat
     6061 aaaaaagaaa cgttggaatt tttagaaata ttagaagatc attctgcagc tatttttgaa
     6121 aaaccgcaaa tacaacagct aacaaatatt gtatccccag ctgtcagggg tgtcttttta
     6181 aaatttctag aaaaagcaga atcggagaaa atcccgatcc aagtagttat tcctgtcgaa
     6241 gtgaaaacaa tcccgattga tctggttgtt tttattcgct gtttatctat ttttatttct
     6301 aacgcattcg atcatcgtgt agccgatcag agtccgatta cgatcaatct gctggaagaa
     6361 aaagagggat cacgttttag tatagcgaac aaggcaaatc caacgaattt gacgctatcg
     6421 gagatggtca aaagaggaaa aacctctaaa aaaaatggcg ggttaggtct atacagtgcg
     6481 aaaaaaatgt tggatgttta cgaaaatgca gaactaaaaa tcgaattttc aaaacagaat
     6541 cactattttt ttgtagaaat gtatctggca tctagaaaga gcaaatcagc ctactgactt
     6601 actgtcaaag gataagtttg aggaaagtct acacggagat agagacaaca acacagcata
     6661 ttcaaaagaa acagaagcgt gatgaccgtt gtcacccttc tgtttctttt gctatgtagt
     6721 taaacaaagt agttgacact tccgctactt agtattagta cgaaagtata cttcatgata
     6781 ctaagtagat aagaagtgaa aagcggaacc cacgatcgtc aaaatgctaa ctgctttgct
     6841 tacaccagat aaagggaaga gcaatatcgc ttattttagg cgtggagaag caagcaaatc
     6901 cctacgatca caaaaaaaat gctgacccag cagttaagag agttagaaca agatgaaagt
     6961 atccatcgaa aagcctataa ccaagtcccg cctaaagtag agtactcgct caccgaagaa
     7021 ggaaaatcat taagagagat tctagtagcg ttgtctgttt gggaagaaga acgtatcgaa
     7081 aaacaaaaag cctcaaggga agaagacacg ctactgcata cacatggtgg ctacctaaat
     7141 tattgatttt ctatgacatt tattttaaat aaaagaaaac aggagttttt ttaaaagcat
     7201 catctatttt ttatgtaaaa aaatggtatg attaatcaag taagaagtga ttggaagaag
     7261 gcaagcaaat gaatagtatg gaaaaagtca accagtttag agatgaacgt aattggcgac
     7321 aatttcataa cgaaaaagat ttggctatct ctatttcttt ggaagcatct gaattgttag
     7381 aactttttca atggaaaacg ccggaagaag taaaagaaac acaattagaa cgaatcaaag
     7441 aagaactggc agatgtattg atctattcat acatgatggc agacaacctt cacctggatc
     7501 tagatgagat cattgaagaa aaattgatca aaaataattt gaaatatcct gtagaaaaaa
     7561 gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg tgtcattcgg ctgttggaaa
     7621 gagcaaaaga aaaacagtaa aatgagtctg gagggagaga aaatgaaaaa aaaaggaaaa
     7681 gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt tattagtagg actagctttg
     7741 attttcaatg aacagatcaa ggattatttt gtcagagaga caggagataa atatgctata
     7801 gcgaatgtaa caaaagaaga tctgaaaaag aataacgata aagatgtcag ttttgacttt
     7861 gatgcggtag aaccaatgac gacagaaggg gtcatgaggt cgcaaatgag aggaacagat
     7921 ctaccagtga ttgctagtat cgcagtccct tcagtttcaa tcaatttacc ggttttcaaa
     7981 ggcttggata atacgtcact actttacgga gcaggaacat tatctccaga tcaagaaatg
     8041 ggaaaaggaa attatgcctt agcaagccat cgtgcaacga accctgaatt gctgtttaca
     8101 ccgctagaaa atttagagat gggtgccaag atctatttga cagatttaga aaatgtgtat
     8161 acatacaaaa cattttttaa agaaaaagtt gctcctacag atacccaatt actgaatgaa
     8221 gtcgagggaa aagaaattgt cacactaatt acttgtggcg acatggacgc cgtcacgcga
     8281 ctagttgtac aaggagaact ggaaagtgtt acatcgataa aagatgcgac agatgatatg
     8341 agatctgctt tcaatctaga gacaaaaaca ttttaggata ctgggacata agtcgttaca
     8401 gattaaaaac agccaaagga tatgaatcga taaggaaaaa ccaatttgct gaaaaaaaga
     8461 gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta gatagacttg tgttccagt
//
LOCUS       DASOUD010000096         8459 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.097, whole genome shotgun sequence.
ACCESSION   DASOUD010000096 DASOUD010000000
VERSION     DASOUD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.097"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..382
                     /locus_tag="U4A29_002190"
     CDS             <1..382
                     /locus_tag="U4A29_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HEO0681303.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="U4A29_002191"
     CDS             complement(776..1783)
                     /locus_tag="U4A29_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0681304.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="U4A29_002192"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="U4A29_002192"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEO0681305.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="U4A29_002193"
     CDS             3679..4002
                     /locus_tag="U4A29_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO0681306.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="U4A29_002194"
     CDS             4013..5695
                     /locus_tag="U4A29_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEO0681307.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            5901..6859
                     /locus_tag="U4A29_002195"
                     /pseudo
     CDS             5901..6859
                     /locus_tag="U4A29_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6869..7105
                     /locus_tag="U4A29_002196"
                     /pseudo
     CDS             6869..7105
                     /locus_tag="U4A29_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            7320..8072
                     /locus_tag="U4A29_002197"
     CDS             7320..8072
                     /locus_tag="U4A29_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO0681308.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
BASE COUNT         2770 a         1298 c         1724 g         2667 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctcttgt cgcacaaagt ttaaatcgct
     6001 ctagacaaac catttataat gtttattcgt ttctcaaaca aggaaaaaag tgctagatta
     6061 ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga
     6121 tgaacaatca acatatgttc aagaaaaggt agctcaaggg tggtcacccg atgtgattat
     6181 cggccgtggc gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa
     6241 aggccttttt gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca
     6301 agaaaagcgt ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc
     6361 acacctgact aaagaatttg gtcatctcga aggcgacacc attgtgggag ttaaacacaa
     6421 gagcgctgtt attacattgg ttgagtgttt atccaaggtt attattactt tgaaaccgac
     6481 aggcagacga gccgaagata tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa
     6541 acacttgttt aaatccatga catttgattg tggaaaagaa ttttccaact ggcgatctat
     6601 aagtaactcg aacgatattg ccatcttttt cgcagatcca ggaacacctt cgcaaagagg
     6661 gctaaatgag cactcaaacg gcttgcttag acgagatggc ttacctaaaa caatggactt
     6721 tagggacatg gatgaaacct ttattcaatc cgttgcctcg aaaagaaacc atattccaag
     6781 aaaatcatta aattatcgaa caccattgga agtatttttg agctgcatag ataaagacat
     6841 attgtctagc ttaatttgac aaatcagaaa agaaaaaatg tatcaagttt ttgaagaaat
     6901 gggttatcca attaaaacgg tcaccagaaa actagaagta atcaaagcgg atgatacgat
     6961 cagcgatttg ttagatgtgg aagaaggaga tccaatgttc tatttccata cacagggttt
     7021 ttcgctagaa cgtctgcccg ttgagtattc gatttcgaaa tatcgaggag acattaacta
     7081 ctttgttttt gaacttgcac aataacttca gattaaataa aatgatttat taaagataaa
     7141 agtcgaatga acaaggctgt cactatgaca gtcatgctcg ttcggtttta ttttttagaa
     7201 tagcagcttg caaattctga aaaattttgc tttccttcct gcagaaggct tcttaagaaa
     7261 acttgttcta gcacttttct taagaatttg atagtcttaa aaagaaaaga ggcgatgaaa
     7321 tgttaaaaac ggatcgattc caagcgatca tgtcgttatt agaggttaac ggtacagtac
     7381 gagtcagcga tattatggag aagttttcgg tttcggatat gacagtccga cgtgatttgg
     7441 acgaaatgga gaaacaagga ttattagaac gcgtccatgg cggagcgaaa ttgaaggatt
     7501 tttatcggca tgtggagctt tctcatagag aaaaacagat tatgctgcaa gaagaaaagc
     7561 agcggattgt ggaaaaggcg aatgaaatga tcaatgatgg tgatacgata tttcttggac
     7621 caggaactac aatcgagctc atgtcaaaaa tcattcaagg ggattcactg cggattgtta
     7681 caaactgttt gcctgttttt gaagaattaa ataagcgaaa agggaatcga aaaatctatc
     7741 tattaggtgg cgagatgcgg gaaacgactc aatgctttgt tggtgaaatc acgaataaat
     7801 cgctacagga tatgaatttt cataaagcat ttttcagctg taatgcttta aatgaacaga
     7861 aaattatgac ttcgactttt gaagagggcc aaactcaaca gatcgctctg aataattctg
     7921 ttgaacgctt tttattgatt gatcattcga aaattgggaa aagagatttt tcagtatatt
     7981 ataatctgaa tgatgtaaca gtagttataa cggatgatga tgaacaaaag cactatgaaa
     8041 aaatcgaaaa agaagaaaaa tatgttgtgt aaatgagaaa ctgccaaact ctagataatt
     8101 agaacttggt agttttttgg tagccttgaa tttaagcgaa ttatgttatt tttagggtgc
     8161 atcattacta attcctccga atacttgaat acaccatttg aaaaaagata gtcgattttt
     8221 ttgggatgtt atgtttatgt gaaggaaaaa atagcagtat caatcaccgc tttattaagg
     8281 aggaaaaggg atatcaaaac aaccatggac actaagtcag ctgaagctct ttgacatgaa
     8341 acgagttaga tcagaaaaag caatgatgaa atcgcacgca taaccgcggg atgcgaagcg
     8401 attcaggaaa aaagaaatca aggaattcgc aattaaacaa caaaaagtca aaaagcaca
//
LOCUS       DASOUD010000097         8374 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.098, whole genome shotgun sequence.
ACCESSION   DASOUD010000097 DASOUD010000000
VERSION     DASOUD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.098"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(463..1011)
                     /gene="aac(6')"
                     /locus_tag="U4A29_002198"
     CDS             complement(463..1011)
                     /gene="aac(6')"
                     /locus_tag="U4A29_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEO0681309.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1564..2667
                     /locus_tag="U4A29_002199"
     CDS             1564..2667
                     /locus_tag="U4A29_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO0681310.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2860..3927)
                     /gene="rlmN"
                     /locus_tag="U4A29_002200"
     CDS             complement(2860..3927)
                     /gene="rlmN"
                     /locus_tag="U4A29_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO0681311.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(4157..6604)
                     /locus_tag="U4A29_002201"
     CDS             complement(4157..6604)
                     /locus_tag="U4A29_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO0681312.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6793..8229)
                     /locus_tag="U4A29_002202"
     CDS             complement(6793..8229)
                     /locus_tag="U4A29_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO0681313.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2354 a         1797 c         1419 g         2804 t
ORIGIN      
        1 atcaacactt ttttttgtct ttttcataaa ataatgattt tttcgtgagg tttcaaggaa
       61 tttttcagaa aaaatagctt tgtacaaggt tttccatggt cttccaaaag ccattaaata
      121 attttcgtta tattgtctat tattgccata caaagataaa aaagaataaa caaaaagaaa
      181 ctgtgacaaa agtcttgtca cagtccctta tctgaataaa cggtgttcaa aagccagttt
      241 ctcggtatta agccaaattt atgcaaaaat gtagaaagcc attttcacaa aatttttctt
      301 actactcgaa actaagcgac tttttcacaa cctctggtac acggtgttcc aaaagtagct
      361 tcttcggaaa taagttggaa tttcacaaaa attcaagaag taattttcgt aaattccttc
      421 ttatttcttg aagctgatta cttctgtcac aacctctttt tattattgag aatttggtcg
      481 aggaataatc gtttttgcca tccaaatatc cggtttgtcc cagccatttg catttggtaa
      541 gacacctacg attttataac ctaatttttc atagaattca tacggatgtt cacgaaggtt
      601 ctggatggaa gccactttat caaatgtatg ctcatacagg tccgtttgac ttaacgttgt
      661 tccatggtct aaatcatccg tacctaaata aatcgtgatt cctcctctgg aagctacttc
      721 tttttctaag taattgacta atcgagtacc tatttggttc tttcgtcggg agctttctac
      781 aactaatgga tgcaattccc aacctgtgat accgtattga gggattgcac caataaatcc
      841 tactaactca tcttggtcta ccgctgctac cgcgattcgt tctggattca tcatttcttc
      901 tacttcttct gccgagctgt ctccatattc ttccggccaa gtcagtctca gtaaatcaga
      961 aagctgatct ttcaatactg gattattacg gtcaaattca ctgattatca tacgtcttcc
     1021 cccttgttct tgcttattct aacagtcctt ttgaaaaaac gaaatgttct cgctcaaaaa
     1081 tctttagcta ccaatcagta tctctattct ttcattattt atcttactta taaaataaaa
     1141 aaattataca gaaacacttt attcaaaata ggaaagatca ggaaagccat caattggagg
     1201 aaaaaactct gtttgttcat catgatatag attatctttt gctttaaaaa aaaccaatta
     1261 tgaattgaca aataaaacct gttttcatat aataatctta atttttagaa tttttagata
     1321 attacaaaaa agcaaaggga atcggtagaa atactaagag gaacaaaaat tcattattgt
     1381 cgttttcaat gtttttgtac ttgttcgttt ttaggatact tattgatgat ttcaaggtgt
     1441 tcatgccacc ttattcatca cttatcctgt cttaggttca cctctactcc atctacagcc
     1501 gcagtcagat cgatcactct ttaaaatttt aacccgtttc aacgaagaaa gaaggaaatg
     1561 aatatgagtc aaagtgtttt cttaccaaat tataccattg gaacagatgc gtatgaaaaa
     1621 gcaaatggaa tcgtcaaaga atatgggaca aaagtagtgc tgattggagg gaagactgca
     1681 ctttcaaagg cagaaaacaa aataaaagta gctttgacac aggaaataga ggttgtcgcc
     1741 agtctctggt acgggggtaa ttcaacttat ggaaatattg aacagttgat ccagcatgaa
     1801 agtgtccaaa aagcagacgt cctgtttgca gtcggtggtg gccgtgcttg cgatactgta
     1861 aaagtcattg gtgaaaaact agctaaacca gtcttcacct ttcccactat tggttcgaac
     1921 tgttcgcccg tcactgcggt atgtgtcatc tataacgaag aaggcgtaat ggacggactc
     1981 ttctttcctt caaaaccacc tgtacacacc ttcattgata cagcaattat tgccgaagca
     2041 cctgataaat acttatgggc aggtattggc gatgctttat ccaaagaagt cgaatctact
     2101 ttttctgctc gtggcgatga tctaggctac acggatagat taggaatcca aatcggaaaa
     2161 atgtgcattg atcgtctctt gacttatggt acgcaagcat tgaaagatag tgccgacgat
     2221 cgtccctcat atgcaattga acaagtcgtt tcggatatca ttgttactac cggattggtt
     2281 tctgttttag ttaacaataa ctataattct gctatggcac actcattcta ttacggtacg
     2341 actgttctag aaaaaatgga gggatacctt cacggagcat ctgtcagtta tggtgtactg
     2401 accttattga tgatggatca gcagttcgat ttgtttgata aaatctatgt gtttatgaaa
     2461 aagaaccaac ttccagtatc gttaaccgac atgaatctca atgtcacgac cgatttagaa
     2521 gcaatcttag ataaagccat ggcaacaaat gatattaaac atgtgcctta tgaaatcacc
     2581 cgagaaaaat ttaaacaggc cattcttgac gtagaaactt atcatgctac acaagaaagc
     2641 cagcaaggat ataagaaaat cagttaaata attgctaaaa cccccatcaa gcaggctgtc
     2701 atcaaattga ggacatttct ttgcttgatg agggtttttc ttgtcaaaaa aagctttata
     2761 gctttcgcta tagaatcttt tctcttattt aagttgaaca gttccttaat taagaaaact
     2821 gctcctttta tggtaattga aaaaacgagt aggatacgat tatttggcaa ctgttttttt
     2881 catttgcttg cttcgcaatt gtccgcaagc tgcatcaata tctgtaccat gttctttacg
     2941 tatcacacag tttacgccgt ttttcttcaa tacatcataa aatctcaata catcagcttt
     3001 tggactgcga gaatattgat catgttcact gactggatta tatggaatca agttgacata
     3061 tgctaatttt ttcttatctt ttaatagatc tgctaattgt tgcgcatgct ctggtctatc
     3121 atttacttga ttcaacatga tatattcaaa cgtgattcga cggtttgttt tctctaaata
     3181 ctcatccaca gcttccatca atttctcaat tgggaaactt cggttgatcc gcatgatcga
     3241 tgttcgcacc tcattattcg gcgcgtgcaa ggaaatagcc agattgactt gcaagccgtt
     3301 attagcgaat tccttgattt tgtgtgctaa tccactagtg gaaactgtaa tgtgccgagc
     3361 accaattgcc aatcctttgg catcattgat gatatgcaaa aagtccatta cattgtcgta
     3421 attatcaaat ggctcaccga tccccattac aacgacatgt gaaacgcgtt cgccaagatt
     3481 gcgctcatca aaataatgtt ggaccatcat gatctgtgca acaatttctc cagcagtcag
     3541 atcacggttc tttttgagca gcccgcttgc acaaaacgta cagccgatat tacatcctac
     3601 ttgtgtcgtc acacaaactg acaagccata ttcttgtctc atcagtacag tctcaatcat
     3661 gtttttatca gggagttcaa acaaatattt tactgtacca tccgaagctt cttggacgac
     3721 catctgtttt agaggattga tgataaaatg ctccgataat ttttcgatca attgtttcga
     3781 tagattcgtc atttctgtga aattacttac acgtttttga tacaaccatt cccaaacttg
     3841 ggcagctcgg aattttttct cgccgttttc tataaaccaa ttgattaatt cttcattcgt
     3901 caagccataa atggactgtt tttccaaagt attttccctc tttttctcaa agtttcgcta
     3961 catactatac atgattcatt tccttaatgc aatacttcac aaataaatcg aatagaactt
     4021 taatctcaca aaaactaaaa agacaagaca tcataccgta ttagaaaaga tggaaaaaaa
     4081 ccattttttc ttttggtatc atcatgcctt gtctttttat tggttattcg tttttcaacc
     4141 cttcatcaca caagttttac atgtcattat cgtccggcaa cgtttctgat gtttcttcct
     4201 gctcaatgaa tactttccga ggcttgcttc cttctgacgg gccaacaact ccatgagctt
     4261 ccagttcatc tactaaacgt gctgctcggt tgtaaccgat ccgaaatctt ctttggagaa
     4321 gggaaatact tgccgtttgc atttcaacga caagtgcttt tgcttcttca aacagctcat
     4381 cttgtggctg ttctggagca cctgtcgttt caacttcatc tgtcggcatc attttctctt
     4441 cataatgagc ttcttgctga tctgtaacaa attgaacgac ccgttctact tcgtggtcag
     4501 aaatgaatgc tccttgcaca cggatcggtt tattctcacc cattggcaag aatagcatat
     4561 ctcctcgccc taagagcttt tctgccccat tagaatcaat gatcgtccga gaatctgtac
     4621 cgcttgatac cgcaaatgcc attctggaag gtacatttgc tttgatgatt cctgtgataa
     4681 catccacact tggacgttga gtagctagga tcatatgaat ccccgctgcc cgagccattt
     4741 gcgctaaacg aataatcgca tcttcaactt cattgctggc taccatcatc aaatcagcta
     4801 attcatctac gatcactacg atgaatggta aggttggatg tttcgttccg ttttccaagt
     4861 ttttctgttg aacaaattca ttgtaaccag aaatatttct tactccggtt gccgcgaatt
     4921 tttcatatcg ttcttccatt tcttgtacta ctttttgtaa ggcttgcgct gcttttctag
     4981 ggttcgtcac gaccggcgtc aacagatgag gtatgccgtt gtacatattc aattcaacca
     5041 ttttcggatc gatcatcatc agttttactt catggggctt agcacgcatt aatatacttg
     5101 tgataatccc attaattgct actgacttcc cactaccagt cgaaccagcc acgagtaagt
     5161 gaggcatctt cgaaagatca gccgtttgta cccgtccaga aacatcacgt cctaaaggta
     5221 cttctagtaa tttatctggg tggtttggtt gtgcttcgat gatttctcta aaggaaacca
     5281 tactgatctt tccatttggt acttcaatac caatcagtga tttacctgga atcggtgctt
     5341 ccatccggac atctttcgcc gctaaagcaa gcgcgatatc atctgttaag ttcacaattt
     5401 tacttacttt tacccctaca gccggctgga cttcgaattt tgtgacggat ggtcctaagc
     5461 tggctttgac gaccttggca tcgacaccga aactttggaa agtttgttct aatacgccga
     5521 tatttttttc aattttttta tactcatcac tttgatccac agttggtata gaatcaagca
     5581 aatcaactgt cggtaattca tagtcttgat tttcctgttc tgcttcaatc tcaaattcta
     5641 atgaggtacc gtcatcctct ggcaactcat ctgaaaaacc ttctgctgta ttggtctgtt
     5701 ttggtgtatc tggtactggt ttctctagat tcgcaggatg gatattttct tgaaaactgt
     5761 cgattggaac aaaacttagc tgctccggct ctgtattttc tggttcctgc cattcatcag
     5821 cagtattttc cctttgctgg ctcaccgctt ttttcttctc atattctgca gcttcttttt
     5881 ctgcagcttc ttttgcagct aagcgccttg cttctttcgc ttctgcgcgc tgtttcattc
     5941 tctcttcctt tgccgcttgc tttcgagctt gcttggcagg atcaccttcc aataattcct
     6001 gtactcgttc tccaattgat tggataccat ttaacagctg atagccttcc agattactga
     6061 acaggaatgc tcctcctgcc agtaatagca ctgctgctaa ataacttcca ggctgtgcaa
     6121 tcaagaagta agtaaaatga taaaggattg ctccaatcat cccaccacca acgttattgt
     6181 ctacttgact ttgttttata tcactggcta acaaatccca tgtcgtcccc atgatatttg
     6241 gttccccagt atggagtttg ccaaataaat gggcatgtaa caataataag ataccgaaat
     6301 aaaagagtat gccgccaaac cagcgtcggt ttttaccaat tgaaaactct gtattcttaa
     6361 tgacaatcca aaatcctaat acggctaata aaagacagag gatttggtaa gtatttcctg
     6421 caatgatccg aaagccgttt gcaatcaata ttcctaaaaa acctaatttt aaaaagccga
     6481 agacagcaaa taaaatcaag gcaaacccaa caccaatcgt cagcatttgt tcctgctgct
     6541 gtttttgctt tttcgttttt ttcgtctttt tccttttttt agtaggccgt ttcttttgtg
     6601 ccatttatta tccctcattt catcaagttt cattatacaa aaaaaagtaa gggaactaaa
     6661 gttttgtttg aaagaactga cgctttcttt catttttcag cttattttac agtttttctt
     6721 aatttctaaa tgattttatc aagaaaaggc acggccatat ccagccgtac cttcttgttt
     6781 taagtatgtg aactaaaggt tctctccatt ttcttcaatc agtttttggt accagtaata
     6841 actgtctttc ttatatcgtt ccaaagatcc ttcttgtgtt tcgtcttgat caacatatac
     6901 aaatccatat cgcttttgat aaccgttcag ccagctaagc aaatctgtat aagaccaggt
     6961 gcaatagcct aggacttctg ctccatccga aattgcttct ttgatggctt tgacgtggct
     7021 ttgtaagtaa ttgattcgat agtcgtcatg gatttgttta tcttctgtca acttgtcata
     7081 ctctcctaag ccattttccg taatcagtac cggcatgcgg taacggcttg tgatacgacg
     7141 cagtccgatc cttaatcctt ctgggtcaat ctcccagtcc caatttgttc gttcgacaaa
     7201 tggattctca acttttttat acattccagg aactccagtt tcttctgatg aacctttttt
     7261 tcctgtcgta ttcatttttc cagcaccaac tccgtttaat ggattgaatg cattcgtcgc
     7321 cgtttgatag taattgatcc caagaaaatc tggtttagca gattctagca attctgcatc
     7381 gccagcttca atcgttggtg cccatccttt ttcttctaag aacctcatgg cagcaatcgg
     7441 atattttccc cacatataga catccatcca ataattggcc atcaggtctt ctgcattttc
     7501 agcagccaga acattttcag gattactgtc aattggataa tttggcgtat acgcaaagct
     7561 tggtccgatt tttccaggca tttctaactc gtgaaattta ttgatgacag atgcatttgc
     7621 taaattagca atatggttaa cttcatacat tcgtttcggg tctgtcacac ctggcggatg
     7681 tgctgctaac aaataaccta atgaagtgaa gatattttgt tcattcaaac taatccagta
     7741 atggactttc ccgcggaatg cctcaaataa tgtttctgca taatgagtga aatcttcaat
     7801 gatctgacgt gattcccaac cattatattc atcttgcaat gcttgtggaa gatcccaatg
     7861 atataaagtc aacataggtt caatatcgtt ttcaatcaat tcgtcgatca atctctcata
     7921 aaaagcgagc ccagcttgat tgacttctcc tcgtccatta ggaaaaatcc gtgtccaagc
     7981 tacagagaag cggtaagctt tcagaccttg ttctttcatt aaagcaatat cttctttata
     8041 acgatgatag tggtctactg ccaaatcacc gttcgttcct ttaaatgtct ttccaggtat
     8101 ccgtacaaat cgatcccaga ctgattctcc tttcccatct tcttgccaag ctccttctac
     8161 ttgatacgct gcagaagcag agccccacag aaaatctttt ggaaatgagg taagttcttt
     8221 gtgttccatt catctcttcc tttcctataa ccttcaatcg ttcctctttt agaataacac
     8281 gaaacccctt ccacaacaaa ataaaaatat gtaaaggagg ttgtgaaaaa gtcgcttagt
     8341 ttcgagtagt aagaaaaatt ttgtgaaaat ggct
//
LOCUS       DASOUD010000098         8230 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.099, whole genome shotgun sequence.
ACCESSION   DASOUD010000098 DASOUD010000000
VERSION     DASOUD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.099"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            329..1252
                     /gene="truB"
                     /locus_tag="U4A29_002203"
     CDS             329..1252
                     /gene="truB"
                     /locus_tag="U4A29_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO0681314.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1256..2197
                     /gene="ribF"
                     /locus_tag="U4A29_002204"
     CDS             1256..2197
                     /gene="ribF"
                     /locus_tag="U4A29_002204"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEO0681315.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2592..3377
                     /locus_tag="U4A29_002205"
     CDS             2592..3377
                     /locus_tag="U4A29_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO0681316.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3390..4241
                     /locus_tag="U4A29_002206"
     CDS             3390..4241
                     /locus_tag="U4A29_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEO0681317.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4365..4817)
                     /locus_tag="U4A29_002207"
     CDS             complement(4365..4817)
                     /locus_tag="U4A29_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0681318.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5282..5650)
                     /locus_tag="U4A29_002208"
     CDS             complement(5282..5650)
                     /locus_tag="U4A29_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEO0681319.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5800..6282
                     /locus_tag="U4A29_002209"
     CDS             5800..6282
                     /locus_tag="U4A29_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEO0681320.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6319..7329
                     /locus_tag="U4A29_002210"
     CDS             6319..7329
                     /locus_tag="U4A29_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO0681321.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7444..8157)
                     /locus_tag="U4A29_002211"
     CDS             complement(7444..8157)
                     /locus_tag="U4A29_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681322.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1667 g         2441 t
ORIGIN      
        1 attaagaaac tgaaaaaata aataaaaaaa aacggaaaaa agactggaag aatggacagt
       61 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
      121 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaaacata
      181 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
      241 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
      301 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttaactttat ggaaagaacg
      361 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
      421 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
      481 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
      541 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
      601 agatatgtct ttatctgaag aaaaaataga tgaagtgatg gcttcattta ttggtgagat
      661 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
      721 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
      781 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
      841 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
      901 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaaaga
      961 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
     1021 gctgccaata gaaaaaggag tgaaacgatt caaacgagta gatattactg atgaagtatg
     1081 gcagaaagta aaaaacggta tgagacttaa ttatactgta tttgcgctcc cagaaatgcc
     1141 gtcagaagaa attgctttgt tttataaagg caaagttgtc agtatctatc agccaaaccc
     1201 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
     1261 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
     1321 agtactagga ttttttgacg gcgtgcatat tggacaccag aaagtcataa agacaggtat
     1381 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
     1441 gattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
     1501 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
     1561 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
     1621 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
     1681 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
     1741 aggtgaaaaa atcagctcta cccgtattcg aggattacta gacagcggac aagtaaaaga
     1801 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
     1861 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cagttcattt
     1921 gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg gaaatttggt atccagccat
     1981 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagaattgta
     2041 tattttggac tttaatcaag atatctatgg tgaacatgtc gcagtacgct ggaatcaatt
     2101 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaagagga
     2161 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
     2221 tttatgtata actagcaatt tcaataaaat gggtgataaa taagcccagg aaaggggctg
     2281 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
     2341 tcggaaataa gccgaaattt cacaaaaatt tgagaagtaa tttttgtaaa ttacttctta
     2401 tttcttgaag ctggccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta
     2461 gcaattcatg aaaacgattg ctaaagcgta tcatttgctt tagactatat atcttggtgt
     2521 acactcactt taagacggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag
     2581 gagtgggtac tatgtataag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa
     2641 aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca
     2701 actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg
     2761 gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg
     2821 atacggcttt agaaaatttt gggacactgg acgtttggat taataatgct gggatggaaa
     2881 atcagcatcc aacccatgaa cttagccttg aagattggga aagagtatta aaagtcaatt
     2941 tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag aatgataaaa
     3001 aaggaaatat catcaatatg tcttctgttc atgaacaaat cccatggcca acgtttgctc
     3061 attacgctgc ttcaaaaggc ggagtaaaac tattcaccga aacggtagca atggagtacg
     3121 caccaaaggg aatcagaatc aatagtatag gacctggtgc tatccgtaca cctattaatg
     3181 cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac
     3241 gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca
     3301 gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc
     3361 aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag
     3421 ttgggtgggg gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga
     3481 tattaggaac tggtattgga gccgtgctcg ttggcttact tgttcaatta ttcgcttctc
     3541 cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca ttctgggtca
     3601 tcggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa
     3661 tttcgactgc attacagtta gtcggaacct cattgattgg tgtcttcgct tttggagaat
     3721 ggtctggaag tatgggaaaa atcattggtg cagcagcaat cattcttctt gtatttggtt
     3781 ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatca
     3841 gtctattagc atcaacaagt attggttatt gggtttacag tgcattgccc aaactagttg
     3901 atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga
     3961 tttatgtgat tttccgaaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacaa
     4021 tagttggagc catcttcagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg
     4081 gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta
     4141 tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat
     4201 tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatc
     4261 tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc
     4321 tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga tacaagtgaa
     4381 atatctgtct gcatcaataa tttttcattg atccattgac taaaatttac gacatgattg
     4441 aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga
     4501 cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggatttga
     4561 ttgattgctg taaaatcacg atactgaagg ttgaaacgca tcgtgattgg tgcatttagc
     4621 atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa
     4681 agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca
     4741 tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact
     4801 gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata
     4861 tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag
     4921 tcagaaaatt ctaacttttc tttctctaaa gaaattcgtt ctctcctaaa gtgatattat
     4981 atttaaatat acctgaaaat aatgggcagt attatgaaag atcactgata ttttctcgta
     5041 ttttttcctt attcaaaagt tttgcaggaa aattgctgaa aaaataattt tactaagtgg
     5101 aaaattcaaa gccattcaat cagtgatttt tttcagcaca agaaagactc cttataaaaa
     5161 tagcagatac tttgtcaatt aagtgtttaa ccagggatga taaaaaggat aggattgaac
     5221 aaaacctgta taagctgatg aatagtttcc tcgccagtaa aaggtttatt cgtataaagc
     5281 tttatcgcca aaatttatca tgaccttttt tatcttcaat attaaatgaa atcaattgtt
     5341 caatcaattc ccagttgatt acacttttcc aaggaacttt gatgacgtta tccgtatata
     5401 cgtagcctgc ttcttggata tttttttcaa gggcacgaat cgctgctttt tccggtgcga
     5461 tagaaaaatg atttttagca aaactaaatg caataataaa ggtaccatgg tcagtaaaca
     5521 taggttggtt ccattttaca gttgtgtcta gctggggata cgtttctgtt atcttagcaa
     5581 aaaggtcttt gatcttttgt tgatgttctt tttctttgat ctgattgatg atcggctgaa
     5641 atttttccat tctaaaacgc ctccattaag tggtttgatt tattttacca gaaaaacaat
     5701 agttatgtat aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata tttatatgat
     5761 aagatgtctc aaataataaa atagaaaaga gacataaaaa tgattgactt ggcaaagaaa
     5821 ttcttacacg aaaatatata ttttgagttg tatcatcatc gagccattta tacaaatgaa
     5881 gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa aagcttattc
     5941 ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa acaaacggat
     6001 ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg
     6061 gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga aaaacagatt
     6121 ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc accaggtaga
     6181 ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca gctacttgag
     6241 atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat
     6301 acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc aacgattggg
     6361 gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga atcagcttta
     6421 aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg gacgcaagat
     6481 aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat gatgcaagat
     6541 gaaaatatcc aaatgatctt tccgccttgg ggaggagaat tattgattga gattctcgat
     6601 tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga tattagtacg
     6661 ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac caaccttatt
     6721 gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca
     6781 agagaaggag aatcgattct acagcaacct tctgaaaagt atcaaaaaga gtggccccat
     6841 gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca atcagtcagc
     6901 ggagatgcag taaggatgaa gggacgatta ttaggtggct gtattgatac gatcagtcat
     6961 ttaatcggta ccccttatgg agatgtccaa tcttttaaaa gaaagtataa tgatggaaag
     7021 ccgatcttgt ggtttctaga aaattgtgag ctatcgtcag ttgatttaag aagatcactt
     7081 gttcagatga aactggcagg atggtttgat ggcagctcgg ggatcatgtt cggtagaagt
     7141 gcagcagagg atttgttaga ttataaaatg aaggatgtat atgaagatct gtcaaaggaa
     7201 ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca actgacattg
     7261 atcaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt tttgcaaacg
     7321 tttatttaaa aaattttttc ctgccttgga gtttatcaaa gaccaacaaa tctaaataga
     7381 aaagaaaatg aaagaagctg acagtatttt gccagcttct ttttgtatga tctattagaa
     7441 attttaatta tccataacta tcacattgat cagctgtttc acatcatttg gattacctgt
     7501 aggaatagct actacagatg cattatcaat aatttcttga gttgtttctc taatgaacgc
     7561 tggataagat gtttgatctg caggcatttg ccaatgtgaa ggaactggat aactagtgat
     7621 tgtcccgtca tggttacttg aatacactaa aactccctgg taactgtatt tcccagtgag
     7681 cataaccaca tctttcggat agactgcact ccctgcatca taaggggcga taggtgttcc
     7741 tgcaggataa tgaataacat gtagttcgaa atcacccgat gtatctctaa aagtattacc
     7801 tctaattgct agccaaacac gtgcgtactc aatttgtaga tcagaatatc ctgctaacgg
     7861 atctattgac tgagaagact gtgaggaagt tgtgtctatt gttgaagaag aaatatttgt
     7921 ctcaactgta ctactagatg tagaagaagt attgggctct gagactttac tgcttgttga
     7981 agatgtattc gtttcagtag aagctgattt ctgagtactt gatttttcag ctgaactggt
     8041 ttgtttgatt gaacttttgt ctgtggtcgc agatggatca ctattttctt tactaggaga
     8101 cgtacatcct acaaggaata aagagaccaa caataaaata aaacctaact ttttcatata
     8161 aaatacctca ttattttttt aataccagta tagcataatc ctgaattttt gaatccgtaa
     8221 aaccttgtct
//
LOCUS       DASOUD010000099         8220 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.100, whole genome shotgun sequence.
ACCESSION   DASOUD010000099 DASOUD010000000
VERSION     DASOUD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.100"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     regulatory      69..148
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            146..1339
                     /locus_tag="U4A29_002212"
     CDS             146..1339
                     /locus_tag="U4A29_002212"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO0681323.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1469..2941
                     /locus_tag="U4A29_002213"
     CDS             1469..2941
                     /locus_tag="U4A29_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO0681324.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3024..3812
                     /locus_tag="U4A29_002214"
     CDS             3024..3812
                     /locus_tag="U4A29_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO0681325.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3879..4547)
                     /locus_tag="U4A29_002215"
     CDS             complement(3879..4547)
                     /locus_tag="U4A29_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEO0681326.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4662..6374
                     /locus_tag="U4A29_002216"
     CDS             4662..6374
                     /locus_tag="U4A29_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0681327.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6377..8149
                     /locus_tag="U4A29_002217"
     CDS             6377..8149
                     /locus_tag="U4A29_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0681328.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2518 a         1380 c         1808 g         2514 t
ORIGIN      
        1 atttttatat tttttaagca aaaaaatgaa aaaatacgag cagaaaactg ttttatctgc
       61 tatacttggt tcgttgcaag ttcccgaaag gatccgcagg acggagatgc cttgtaaccg
      121 aatttaacta gggggaatca ttataatggt agaaagacac ttatttacat cagaatctgt
      181 ttctgaagga catccagata aaattgctga ccaaatcagt gatgcaatct tggatgcaat
      241 tttaaaacaa gatccaacag cacgagtagc gtgtgaaacg tctgtaacaa ctggtcttgt
      301 tttagtattt ggagaaatat ctacgacggc atatgtagat attcaaaaag tcgtacggga
      361 aacaataaaa gaaattggtt atacacgtgc aaaattcgga tttgatggag atacagcagc
      421 tgtattggtt gcgattgatg aacaatctcc tgatattgct caaggagttg atgaagcact
      481 tgagatccgg gatgaagata aaaaagacgt attagatgag attggtgctg gtgaccaagg
      541 tttaatgttt gggtttgctg tagatgaaac accagaattg atgcctttac caatcgcttt
      601 gagtcatcgt ctggtacgac gcttggcaga tttgcgtaaa tcaaatgaat taacgtattt
      661 acgtccagat gcaaaatctc aagtaacggt tgaatatgat gatcaaggac aaccggaacg
      721 cgtagataca atcgttattt caacacagca tgatgatgca gtagataatg aaacaattcg
      781 tcatgatgtc atcgaaaaag tagtgaagga agttattcca gctgaattat tagatgatca
      841 aacgaaatat tatatcaatc cgactggccg atttgtcatt ggtggtcctc aaggggatgc
      901 cggattaaca ggaagaaaaa tcattgttga tacgtatggc ggttatgctc gtcatggtgg
      961 cggtgctttt tctggtaaag atgcgacaaa agttgaccgt tctgccagct atgctgcacg
     1021 ttatattgct aaaaacattg ttgcagcagg gcttgctcgc aaagcagaag tacagctagc
     1081 ttatgcgatt ggtgttgctc aacctgtttc gatctcaatc aatactttcg gtactggaac
     1141 ggttccggaa gaagaactga ttgctgcagt gagagaaaac ttcgatcttc ggccagcagg
     1201 aatcatcgag atgcttgatt tgcgtcgtcc gatttacaaa caaacagctg cttacggtca
     1261 ttttggtcgt acagatgtag atttgccttg ggaacatacg gacaaagtag atgcgttgaa
     1321 agcaagttta gcaaaataag ttatattcag ctggaacgat cgccaagtgg tggttgttcc
     1381 agcttttttt atggcagtta acctcttgaa aaagatagta attatttatc tatttttaga
     1441 tcctgtcata atgaaaggga ggaataagat gaaaaaagaa acaaatgtta ctttggtcac
     1501 agtgagtatt ttcattgcaa cgttcatgac ggctattgaa ggtacaatcg taacaaccgc
     1561 tatgccaacg attgtcggtt cattacatgg aatggaaatc atgaactggg tattttcgat
     1621 ttatttgctg acaaacgcta tgcttactcc gatttacgga aaacttgcgg ataaagtggg
     1681 acggaaacca atctttattt ttgggacatt attgtttatt atcggttcgt tattttgcgg
     1741 tttatctcaa agcatgattg ggttgattgc tgctcgtgcg attcaaggga tgggtgctgg
     1801 ggcaatgatg ccagtagctt tgacgatcat cggcgatctt tattctattg aaaagcgagc
     1861 aaaagtttta ggtttgaata gttcagcctg ggggattgcc agtgtttttg gaccgcttgc
     1921 gggaggattc atcgttgaaa cgatcagctg gcactggatc ttttttatca atgtgccgat
     1981 cggaattcta ttaattattt tgatttggct atatttaaat gaaccaaaaa tcatacatga
     2041 gtctaaacca atggatattt ttggtagttt tagtttgatg gccatgcttg tctcattgct
     2101 gttagggttc caattattag gagacgaagg aatgagtccg cttgtttctg ctttgctgct
     2161 aggtagtatc gtaatgttag gtatttttgt ttttgccgaa aaaagagcta aagatcctgt
     2221 catcatgatg catttattca aaaatccact atttgttgtg gtcaacttgg ctgcagcatt
     2281 ggctagtggt tttttgatgg gaatcgatgt ttatatccct atgtggatgc aaggtgtatt
     2341 agggctgaat gcagcaattg gtgggctggt attagcacca ttatcactag tctggatggt
     2401 cggttctttc cttgcaagca agtggatgac tcaaatgaaa actcattggg tcttgagaat
     2461 tggactacta atcgcagcta ttgggggcgc ttggttgtat cttatgcctt tcagaagtaa
     2521 ctatttatgg ttttttgcta tatcaacggt catcggtata ggcttaggtg tgaccatgac
     2581 tacctcgact gtcagtgcac aaaatagtgt atctcgtgac cagcttggtg tagcaacttc
     2641 ttttaatacg ttagttcgaa caattggtca aacagtcatg gtttctattt ttggtttatt
     2701 gataaatggg gtaactaaac gagaacttgc agcagcaaat ttaacaaatg atgcagatat
     2761 catgaataaa ctggtgaatc cacaaacggc taaatatatt gatgcggata ttttgttacc
     2821 attaagaaaa attctttatg atgggttaca ttccgtttat ctagttggct tgttactcgt
     2881 gatcattgct ttggtcttaa cgcttttcgt tcatgaaaaa agtggtaaaa taaaacagta
     2941 gtttcggtta agatatgtac gtagccgaat cattcaaatt taaagttatt caaaaataac
     3001 ttcttactaa ggaaggcgaa aatatggcat ttattcaagc aaatatttat tcgaatgtgt
     3061 tagaaatgga agtcaatcta aatgtgatac taccgcaaac aacaaaaaag gtgattggca
     3121 ccagttcaga gcaagagaca acggatatac ctgttttata tttacttcat ggaatgggtg
     3181 gcaatcatag tgtatgggaa agacgtacgt cgattgaacg ttatgtagca aactacggat
     3241 tagctgtcat catgccatct actgatcttg gctggtacac agatacagtt tatgacatga
     3301 agtattggac attcattgca gaagagctac ctgagatttg tcatgaatta ttcccgcaat
     3361 taacaagaaa aagagaaaaa acatatgccg ctggattatc gatgggcgga tatggcgcgt
     3421 taaaactagg ccttgcaaaa tcagaaaatt tcgcagcagt aatctctctt tcaggcgctg
     3481 tttctgtagg aaatcgaatg gatgatcttt tgatggttcg taaagcatct ttttgggaag
     3541 ggatctttgg accattggat caaatcgaag gttcaaaaaa tgatccgcaa tacttattga
     3601 aagaactagt aaaaagcgga aaaccagttc caagatttta tatggcttgt ggggaatctg
     3661 attttcttta tcaggcaaat caggaaatgg tatctgctat gcaggaacaa ggcttggcgg
     3721 tcacctttga acaaggacct ggcgatcata actggatctt ctgggaccaa tggattcaac
     3781 gtgcattggc gtggctaatg gaagaaaaat aaaaaaagag tcttaaaaag atagctccta
     3841 tagagatcac aaacatagtg atctctatag gagctttttt actgctcgtt ttctcttctt
     3901 ttattttgtt ggcataagcc ttcaatatag gtttgtgcac tcgttagaaa gagtgctttt
     3961 tcattcaatt ctgcatggga ctgcatactt tctttaaatc cctctaattg attttcaact
     4021 acataattac gcataaatag aactgtttct ttcaaaaatt catttccgtc cataattttt
     4081 tgtcgaagct gctgttcatg ctgctgatcc attaactgtc caatcaataa attgtggaga
     4141 gaggcaatgg cctgatgact ttgggtgact gaaaatcctt cacgaacaag taattggatc
     4201 atttgctcca aatgttctaa tcgactcgga aaagcgggca cagtattgat ttctaattct
     4261 gccccacaag ggaaacgtgt ataaaggtca tagtaatttt ccatatactg cagcaactgt
     4321 tcattccaag gcaaagttgg atctggaagc gctaattctt cttccattgt ttcagccatt
     4381 ttttgcaaga gctgttgttt gtttttgaaa taccaatata aagcaggtgc ttgaacatga
     4441 attctgtttg cgatgtttcg catggaaagc tgcgaaagag cttgtgtttc agccaggata
     4501 tcaaaggcgg cctgaatgat gattgcttga gaaagcttta tttccatttt atcaacctcg
     4561 tgtattgcat tatcttttta aacatggtaa attgttagtc ggtctaattt tacagtgtta
     4621 aattagtcct gtcaatttaa acaaaggggt gtaatcaacg aatgaaatta atcttacgac
     4681 atgcgaaaaa atataaagta gctgttttta tttccttgct gtccgtagca gtcatggttg
     4741 cagcagcatt atggcaacct aagttactac agcaagtatt ggaagctatt attacagaag
     4801 ataatgatga aatgaaaaca attggtattt acctgatcag tctggctttg ctaggattag
     4861 ccgcaggtgt aacgaacacg attttctctg caaaagtagc tcaaggcgta agtgcagata
     4921 tccgagaaga agcatttcga aagatccaga cattttcatt tggaaatatc gaacagttct
     4981 ctgctggaaa tcttgttgtt cgtctaacaa atgatatcac gcaaatccaa aatttagtga
     5041 tgatttcttt gcaatcactt tttcgaattc cattcttatt tattggtgcc ttcattttag
     5101 ctatgatcac gatgccgcaa ttatggtgga tcatcgtagc gcttatcgtt acggtatttc
     5161 tgattacggc actttcattt acaaggatgg gcaagcactt catgatcatt caaaaactga
     5221 ttgatcgtgt gaatagcatt gcaaaagaaa acttactagg gattcgtgta gtgaaatctt
     5281 ttgtacaaga agaaaaccaa ttgaagtcat tcagcaaagt aagtgaggac ttaacaaaac
     5341 acaacatcat agtcggtaca ttattttctg tgatgatccc gtccttcatg ctggctgcca
     5401 atttggctgt ggttggggcg atcttttttg tgagcgatct tgttaaagat gatcctacgt
     5461 taattggtgg aattgcttca ttcatgaact acttgatgca gattatgatg gcaatcatta
     5521 ttggcggaat gatgatgatg atgacttcaa gagcagccgt atcgctgaag cggattgcgg
     5581 aaattttaga tgctgaacca gatcttactt atttagacgt tccagaacaa gagttgacgg
     5641 gttctgtcac ttttgaccat gtttcttttc gttatcctgg tgaggataca gatacgctta
     5701 aagatatcag cttttctatc aaacctggag aaatggttgg tatcgtcggg gcgactggtg
     5761 ccgggaagtc aacactagca caattgatcc ctcgtctatt tgatccgaca gaaggcaaag
     5821 ttgaagtagg tggtgtagat cttcgtcaag taaatgaaaa gagtttaaga caaacggttt
     5881 cctttgtttt gcaaaaagca attttattct caggaacgat tgctcaaaac ttgcgccaag
     5941 ggaaaaagaa tgcaacagaa aaagagatgg aacatgcttc ttctattgct caagcaaaag
     6001 aattcatcga aaagttatct gatcgttacg aagcaccaat agaagaacgt agcagtaact
     6061 tttctggtgg tcaaaagcaa cgtttatcca ttactcgtgg cgttatcggt aatccaaaga
     6121 tcttgatcct agatgacagt acgagtgcac tggatgctcg ctctgaaaag ctggtccgag
     6181 aagcactcga tcgagatttg aagggaacaa cgacgattgt catcgcacaa aaaattgctt
     6241 ctgttgtcaa agcagaccgc atcttagtat tggatgaagg cagattagtc ggcgaaggaa
     6301 cccatgagga attggtagct ggaaaccgtg tctatcagga aatctttgaa actcaaaaag
     6361 gaacggagga gtaagtatga ctgatctaat aaaagcaagt aaatttttct atcactattt
     6421 gaagcggtac aaactctcct ttttctttat ctttatcaca gtgatcattg ctacctatct
     6481 acaagtaaag gcacctcaat acatcgggga agcttttcaa gaattagcta attacgtagg
     6541 aggcttgatg caaggcgtag acgataaaag caaatttgtt tctgtaatat ggaaactctt
     6601 gctgttttat gtactagcta gtgcagccaa ctttatctat agtattttgt tcacacaagt
     6661 agtagggaag tcaacgaacc gaatgcgtat cggtctattc aacaaattgg aaaaattgac
     6721 gatccgtttc tttgattctc atcaagacgg tgaaatttta agtcgtttta ccagtgactt
     6781 agataatatt caaaatagtt tgaaccaagc tttgcttcaa gtcatcacga atgctgtttt
     6841 acttgtcggg atcttgatca tgatgttccg ccaaaatgta gagttagctt gggctacaat
     6901 tgcttccact ccagtagccg ttttgatagc tgtcttgatt atccgcaaag cacggaaata
     6961 tgtggatctt cagcaagatg aagtggggaa actaaatggt tatatggatg aaaaaatcag
     7021 cggacaacgt gtcatcatca caaatggctt acaagaagaa acgattgaag gattcttaga
     7081 gcataatcaa agtgttcgcg aagcaacctt caaaggacaa gcttattctg gtctactatt
     7141 tccgatgatg caaggaatgt cactggtcaa tacagcgata gttatctttt tcggcggctg
     7201 gttagcatta aatggtgatt tggaacgttc ggtggcatta ggcttagttg tgacattcgt
     7261 ccagtattca caacaatatt accaaccact gatgcagatt tcatctggat acagcatgat
     7321 ccaattagcg attacagggg caagacgatt gaatgaaata tttgatgaaa aagatgaagt
     7381 caaaccagaa aacggtaaag catttgatgg tgttgaaaaa ggtcttgcgt tgcagcacgt
     7441 agattttggt tattctccag aaaaactagt cttgaaagat gtctcgatca atgtggacaa
     7501 aggagaaatg gttgctcttg ttggtccaac aggatcgggg aaaacgacga tcatgaatct
     7561 gctaaaccgt ttttacgatg tcaatgatgg ttcagtagtc atcgatggaa tagatatccg
     7621 agattatgat cttgatgcat tacgtagaca tgtaggaatc gttttgcaag aatctgtatt
     7681 gttctccgga acgatcaaag acaatatcac attcggcaaa ccggacgctt cagatgaaga
     7741 agtcatcaat gcagcaaaac aagcgaatat tcatgacttc atcatgacgt tagaaaaagg
     7801 ttatgataca gagatatccg aagaaaataa cttattcagt acaggtcaaa agcagttgat
     7861 cagtattgct cgaacgatca tcacaaatcc atctttattg attttggatg aagcaacaag
     7921 taatgtagac acggtaacag aagcaaggat ccaaaaagca atggatgaag caatcaaagg
     7981 aagaaccagc tttgtgattg ctcaccggct gaaaacaatc ttgaatgcag atagaatcgt
     8041 tgtcttgcgt gatggggaag tcattgaaga aggcagtcat catgagttat tagcagaaaa
     8101 tggattttat gcggaacttt atcataatca atttgtattt gaaagttaaa ttaaaagaac
     8161 ctcgttataa cggggttctt tttatatatt ttatgttgtt tgtttttata aggggcagaa
//
LOCUS       DASOUD010000100         8133 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.101, whole genome shotgun sequence.
ACCESSION   DASOUD010000100 DASOUD010000000
VERSION     DASOUD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.101"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            386..574
                     /locus_tag="U4A29_002218"
     CDS             386..574
                     /locus_tag="U4A29_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEO0681329.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            652..1665
                     /locus_tag="U4A29_002219"
     CDS             652..1665
                     /locus_tag="U4A29_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO0681330.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1732..2766)
                     /locus_tag="U4A29_002220"
     CDS             complement(1732..2766)
                     /locus_tag="U4A29_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0681331.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2881..5106
                     /locus_tag="U4A29_002221"
     CDS             2881..5106
                     /locus_tag="U4A29_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEO0681332.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5124..7007
                     /locus_tag="U4A29_002222"
     CDS             5124..7007
                     /locus_tag="U4A29_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEO0681333.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6988..7962
                     /locus_tag="U4A29_002223"
     CDS             6988..7962
                     /locus_tag="U4A29_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEO0681334.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2631 a         1479 c         1711 g         2312 t
ORIGIN      
        1 tttagttagt ctacattatt ttggcgattt gccaataagg actgttgagt tgtgcaaaat
       61 caaatctggg tcagaatgaa cgtcattctg tctcagattt ttcgttattt caatttgatt
      121 aactgattga tcaaaaagat ttttattttc atattctcaa atgattcaac actaaatata
      181 catgtttgac gcggaaatta ttattcgatt ttggcggcat caggggggat gacgacgtag
      241 cagacccatt attttactca agaaaatgtc cgtgcctatt atttagaata cgatagtgaa
      301 cgtgctggga gttttgttct tttagagaaa gtctctgaag ataaattagt tgtcttagat
      361 tttgactgta acgaaaaatg gcgaaatgga agagcaccag caagtgattg accggatcta
      421 cgaagcgaca cgatatctgc ctttggatcg tttgtgctta agtcctcaat gtggatttgc
      481 ttcaacagaa gaagaaaatg ttttgtttga agaacaatgg aaaaaatttc ttttgtcaaa
      541 agcattgcag aagaagtttg gggaaaatca ttaaatcatc taggagagct tgataggaaa
      601 taacactatg gtataatcag aatgcttaat actaactact gagggagcct tatgaaaaag
      661 aaaattctta aatttatcgg atggttcttc ggaatcattc ttgggatcgc gtttgcaatc
      721 tttcttgcct tccagctttc ccccagacct ggagcattct tgatcaacca tatgttttca
      781 aatactgttc aaatcactga taaaaaaacg ttcgatcaag cagaaaaaaa cgtggcgaaa
      841 aaaacagatt tgacgtatga gtccaagtat aaagagaata cttatgacat ctactatcca
      901 aaaaacagca aggggcctgt tcctgttcta ttttgggtac atggtggagg attcgtcggc
      961 ggagataaat cgggtgtcaa agaatttgcg acaaaattag tttcagacgc caacatcgca
     1021 gtggttgcga tgaattatga attagcgcct gattcagaat accctaatca agttcttcaa
     1081 gtgaatgaac tgatcaaaga tttattagct gaagaaaaag acgacaaatt gctagacatg
     1141 gaacaattat tttttggtgg agatagtgca ggttcacaaa tcgcattgca atatgcagct
     1201 atacaaacga atgatgcgta tgctaaacaa atgaaaatca aacagctact tccaaaagat
     1261 agtctgaaag gaaccatttc ttattgcgga cctgttgatt tgaagctaac agcacagcag
     1321 cattcggatg atcgatttat gaagtttttc gtcaaaaccg ttgcgtggtc gttgattgga
     1381 acaaaagatt ggaaaacgag tgagcaattg aaagaagcta gtgtggctga ccatgtttct
     1441 aaggaatttc cgccaacgta tataactgac ggaaatgcat actcattcca ggagcaaggt
     1501 caagcattag aaaatcggtt gacagaatta aaggtacccg tgcaatcctt gttttataca
     1561 gatacaaaaa aagagattac ccatgaatac caatttgatt acacgctaaa agaatcgcaa
     1621 aattgttacc agcagacatt ggattttgtg aataagtata agtaagaggt tgtgaaaaag
     1681 ccgtttcgtt ttgagtatga agaaacgaag cggcttttca caacctctct ttcaatacct
     1741 ttttgttctt gggataaaac tttccctaac aatcaatcgt gcaggataag tcaaaatgat
     1801 tggttcgtcc cgtataccgt caattcgctc caatgccatt cgaacagctg cttgcccaat
     1861 tgatttcgca ttgatttgaa cactggtcaa gccaggggag aggtttgcga ttgtctcgat
     1921 attatcaaag cttgctacaa caatctcttc tccaatttgg atattttttt gtttcaatgc
     1981 ccgatagaca ccaattgcca ttggatcgct cccgacaaga atggcagtag gaagagggga
     2041 ttcattttct aatagattag tcgcaagtac ctcaccttgt ttttctgtcc agccacctag
     2101 atgataagac atatattttt ctaagtgatg atcgatcata aaatgctgat aggcacgcat
     2161 cctcttttca tttatagaaa tctgcttccc accgaattca tccatatcga ctttatatcc
     2221 cccgatatag gctattcttt tatgaccatt ttctaagaac aattccagta cggttttagt
     2281 catccgttca agatctgcat acaccatatc gacctcttgc tggatgtcag ggttatccac
     2341 gacgactatt tgcatgtttt gttttaacaa ggcggtaatc gattctttcg taacagtccc
     2401 aacaacgatc accgcaccta actgatctag atcttcccat tgtttaggat tatcagatag
     2461 attatagatt cgattcatcc ctaaatgcag acgtcgagct tcttcttcca tccctactcg
     2521 caaatgtttg taatagatat cctgttcttc gctcgctttg ccgatcgtag agatcacacc
     2581 aatcgaaggc attctctttt tgatatattt acgagtactg atgtcataat ccaacgcagc
     2641 agctgcctct actatccgtt gccgagtagt ctgagccaca tggatcgtct gatcgttatt
     2701 caacacacgg gaaactgtag caggagagac atttgccttt cgtgcgacat ctctaattcc
     2761 aaccattttt acacactcct caaaaaaata aaaaattttt attgacaaaa ttgtttagta
     2821 aacagtatac aggaattata aaaaaaagta aacggtttcc gttttgttgg gagtgaaatg
     2881 atggatatta cgcatagaaa aacagataaa aatttcatct tatttgatga aaagagccga
     2941 gtcttccatt tacgaaatac atttctttct tatctgataa agatagaaga aagcaatgta
     3001 ttggcgcata tttattttgg caaaccagtc aagcagtaca aagacaataa aaattatccc
     3061 cgaagagatc gtggattttc aggaaatgtt cccttaaatc ctgaccggtc tctgtcgaaa
     3121 gatacactac cgcaagaata ctcttctcat ggcagcatgg actttcgaac gcccgcatcg
     3181 atcattcagc gcaagaatgg atcagactta ctggatctga gatatgacag ccattatatt
     3241 acagatggga agccagagat cgaaggcttg cctcaaactt atgtgatgga caagtcagag
     3301 gcacaaacgt tggtgatttc acttaaggat agagaaacag cgatttattt cgatttgttc
     3361 tataccatat ttactgatag agcagtgatc acacgatctg taaagataag aaatgaaacc
     3421 ggagaaacaa tcaaactaga aaaagcggct agtttccagc tagactttgc ccatactagg
     3481 cgatttgatg aagtcattgc tttgccagga gcacatgtca atgaaagaca gatttcacgg
     3541 caatctgttt tatctggaac aaaagtattt gaaagccgtc gaggaacgag cagtcaccat
     3601 atgaataact ttattgcttt agttcatcat catacgacag aaaatactgg agaagcgatc
     3661 ggacttcaat ttgtttattc cggcaaccat tctttcgaac ttgaaaaaga tcagatcaat
     3721 caattgagag tcgtcggtgg gatcaacagc catcgttttt cgtgggaatt gaatgcaggc
     3781 cagtcttttc aaacacctga aatgatcctt tcttactcaa gtcagggatt gaacaaaatg
     3841 agtcagatcc accatgagtt gctacgtgaa agaatcgcaa gaggtcgtca tcaatttgct
     3901 gaacggccga ttctggtcaa taactgggaa gccacttact ttgacttcaa tagtgaaaaa
     3961 atcaaggcga tcattgatga agcaaaggag ctaggcatcg aaatgttcgt attagatgat
     4021 ggctggtttg gaaaaagaga tgcggacaat tcttcattag gagactggtt cgagtatgaa
     4081 ggcaagttga caaatggatt acgtgaaata gcagattatg ctcatagcaa aggattgaaa
     4141 tttggtttat ggtttgaacc tgaaatgatc tcggttgatt ctgaactgta tcgaacacat
     4201 cctgattttc ttatgcagga gccaggtaga atgccttctg cttccagatc acaacatgta
     4261 ctggatttta caaggttaga tgttcggcag acgattgaga aacaaatgag aaagatttta
     4321 gatacgattc ctcttgacta catcaaatgg gatatgaacc gttcgctttc agatgtctat
     4381 tcgattaccc ttgacccaca gagacaagga gaagtagcgc atcgttatat gctcgggctt
     4441 tatgaactac ttgaacattt gacaactgat tatccggaaa tcttatggga aggctgttct
     4501 ggtggaggtg gacggtttga tgccgggttt atttattata tgccgcagtc atggacaagt
     4561 gataacacgg atgcagtgga gcgtatgaaa atccaatatg gaacaagcct tgcttatccg
     4621 atttcgtcca tcacagctca tgtatcagct gtaccaaatc atcagaccga cagatctact
     4681 tcgttgaaaa caagaggaga aacagctatg agcgctgttt ttggttatga actggatttg
     4741 acaaagctgt ctcctgaaga aaaaaagcaa gtaaaagacc aaattatatc ctatcaaacg
     4801 attcgtccgg tgatccagta tggccattat tatcgtttgg cgagtccttt tgaagaaaat
     4861 attgccgcat ggatgtttgt gtctcctaaa caagatgaag ctattgtgtt tttagggagg
     4921 atactagctt cagctcaacc agcgttccat gaagtatatc tgatggggtt agatgatgag
     4981 gcactttatc aggaacagac ctcgaagcgg atattttcgg gggccgaatt gatgacagtt
     5041 ggactttact tccccgattt tcaaggtgat ttccaaacag aactgcttca tttcaaaaag
     5101 ttatgagaga gaaggaaaaa agtatgaaag caaatataaa atggttagat gatccacaag
     5161 tttttagagt gaaccaatca aatgcacata gcgatcatgt ttgttatgca acaagggaag
     5221 aagtagaaac cacatcctcc cttaaacagt ctttaaatgg atcatggcgt ttttgttatt
     5281 cgccaaatcc ttcggtacga ccaaaaacat tttataaaaa agattttgat gcctgttctt
     5341 ttgatgaaat cactgttcct agtcatattg aattagcaaa ttatgacaag atccattaca
     5401 tcaatacgat gtatccgtgg gagggacatg tatttcgccg tccccctttc acgacaagcc
     5461 ctgagaaaga ggcggaaggg atcttttcag ctgctaaaga caatccggtt ggttcgtatt
     5521 tgacgacttt tcgattagaa gaaggtctaa agggaaaaca aatctcgatt cattttgctg
     5581 gtgtagaaca agcaatgttt atttggttga atggtcattt tatcgggtat gccgaagatt
     5641 cgttcactcc gtctgaattt gatttaacac cttatttgga agaagaaaat attctagctg
     5701 tggaggttta caaaagaagt acagctgcat tcttggaaga tcaggacttt ttccgatttt
     5761 ttgggatctt cagagatgtc ttcttagttg ctcgaggaca aaaccattta gaagatcttt
     5821 ggatcaaacc tcgcttagat gacaaatgtc aaaatggcaa acttgaagta gatttgtctt
     5881 ttgagcaagt agccaaaaac agtcagctga atgtagcgat actagatcct gatgggaagg
     5941 aaatctatca acagcaatat ccgttaaaag agcaaatgac gattcgcatc gatggatttg
     6001 aaaatgtggc actgtggggg cattttcagc ccaatttata taagcttgaa ttggagacga
     6061 tagacgaaga aaaaagagta acggagtata ttgactattc cttcggtttt cgtaaaatcg
     6121 aaatcaaaga taaaatcatc tacttcaatg gaaaacggat gattatcaac ggggtaaatc
     6181 gacatgagtg gcatccgaaa aaaggacgcg ccatcactat agaagatatg gaaaaagatt
     6241 tggctatcat aaaaaatgca aagatcaacg gcgtacggac atctcattac cccaaccaga
     6301 tcacttggta ttatttatgc gaccaagcag gaatccatgt gatggcggaa acgaacctcg
     6361 aatcccatgg ctcatggcaa aaaatgggcg catttgagcc ttcttacaac gtaccaggtg
     6421 cagtacctca gtggcaggcg gctgttcttg atcgagcaaa aacgaatttc gaaacattaa
     6481 aaaaccatcc aagtattctt ttttggtctc ttggaaatga atcttatgcg gaagaaaaca
     6541 ttcgacaaat gaacgattac ttcaaaaaag tagaccccga tcgacttgtc cattatgaag
     6601 ggaattttcc aaatccagca ttcgaagatt cgatttcaga tgtaaaaagc tgcatgtatg
     6661 cgccaccaga agagatcatt gactaccttg aaaataaagc ggaaaaaccg ttcatcttat
     6721 gtgaatatat gcatgacatg gggaattcgt taggtggaat ggattcttac atgacgttgc
     6781 ttgatcgtta tgaaatgtac caaggaggct atatatggga ttttaaagat caggcgatcc
     6841 aagtgactga tccaatcaca ggcagagaag tgatgcgcta tggcggtgac tttgacgata
     6901 aaccaagtga ctatgaattc tcagggaatg ggatcgtttt tgcagatgga caagaaaaac
     6961 ccgccatgca ggaggtaaga tattattatg aaaaatacag taaataaaag tcatatggat
     7021 acggaaaaag ttgcaatcgt cttcggcgac tgtacattag gtgtcaaatc ggggaatacg
     7081 cattatattt tttcttatac aagaggcgga ctggaatcgc tcaataaaaa cggaaaagaa
     7141 tggctatacc gggaaacact tccagttttt tggcgagcaa cgaccgataa tgacagaggt
     7201 agcggatttg gatttaaatc cggtatgtgg ttaggagccg gattgtatcc aaaagtcaaa
     7261 gaaatagaag tactggtgga caataaaaag atcgatttgc cgattgctcc agtaaataat
     7321 caatattcaa gtaatgaata cgctgaaacg gttgaaatca tttttacact ggaaatcaac
     7381 acagtaccaa aaacccaagt gacaatttcc taccaaatcg ctggagaggg taaaatgctt
     7441 gtcaatatgc attattatgg acaaaaagga ttaccggaat tacccgcatt gggtttaagg
     7501 ttcattatgc caacgaaagc aaaatttttt gagtatgaag gcctatcagg ggaaacatat
     7561 ccggatcgga tggctggtgg tgtcgaaggt atttataaag tggcaggact ccccgtttcc
     7621 ccataccttg ttccccaaga atgcggcatg catatggaaa cgaaatgggt agaactaaca
     7681 agaaatcaaa cgttggataa tacggataaa gatcaagaag atttttcgtt gcgatttgaa
     7741 cagcaggata cgccatttgc tttttccgct ttaccattca aagcagaaga actggaacag
     7801 gcaacacata tagaagaact accgccagaa agacgaacgg ttcttacgat ttttgcaaaa
     7861 gtaagaggag tcgggggtat caatagctgg ggagcggatg ttgagtcacc ttaccgaatc
     7921 agcagcgaag aggaccatga aatcggcttt attattcagt agcaatcttt agattattat
     7981 ttgaaaaacg gagtttacaa gacaaagctg ctttattcta tatgtcatga acaagagagt
     8041 aaaaaagttc tcagatactt ttgttcatga ctattttctt caataaaacc aattgttatt
     8101 tttctgaaaa acaaagttaa ctatcagaaa att
//
LOCUS       DASOUD010000101         7992 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.102, whole genome shotgun sequence.
ACCESSION   DASOUD010000101 DASOUD010000000
VERSION     DASOUD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.102"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            48..1787
                     /gene="yfcC"
                     /locus_tag="U4A29_002224"
     CDS             48..1787
                     /gene="yfcC"
                     /locus_tag="U4A29_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEO0681335.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWAIFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1944..3128
                     /gene="iadA"
                     /locus_tag="U4A29_002225"
     CDS             1944..3128
                     /gene="iadA"
                     /locus_tag="U4A29_002225"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEO0681336.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3239..3442
                     /locus_tag="U4A29_002226"
     CDS             3239..3442
                     /locus_tag="U4A29_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO0681337.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3543..4205)
                     /locus_tag="U4A29_002227"
     CDS             complement(3543..4205)
                     /locus_tag="U4A29_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO0681338.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4218..5720)
                     /locus_tag="U4A29_002228"
     CDS             complement(4218..5720)
                     /locus_tag="U4A29_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0681339.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6268..7947
                     /locus_tag="U4A29_002229"
     CDS             6268..7947
                     /locus_tag="U4A29_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO0681340.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
BASE COUNT         2470 a         1427 c         1733 g         2362 t
ORIGIN      
        1 attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa
       61 attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca
      121 ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt
      181 tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt
      241 tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat
      301 tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat
      361 tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct
      421 ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa
      481 aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa
      541 attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg
      601 cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg
      661 caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat
      721 tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat
      781 tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa
      841 ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg
      901 tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga
      961 ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa
     1021 tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata
     1081 tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag
     1141 agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc
     1201 cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt
     1261 tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg
     1321 atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag
     1381 gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt
     1441 tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca
     1501 acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac
     1561 tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag
     1621 gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac
     1681 ggatcgaatg ggcaaaatgg gcaatattcc aaatcaagat gcaaggattc ctgtttgttt
     1741 taggaactat tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg
     1801 taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa
     1861 aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat
     1921 acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag
     1981 attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac
     2041 agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac
     2101 taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt
     2161 atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag
     2221 ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag
     2281 cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc
     2341 cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg
     2401 gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg
     2461 ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca
     2521 atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa
     2581 cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg
     2641 aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc
     2701 ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga
     2761 tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc
     2821 cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc
     2881 tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag
     2941 ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg
     3001 gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg
     3061 ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa
     3121 gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat
     3181 ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat
     3241 ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga
     3301 agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact
     3361 aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc
     3421 tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa
     3481 tccgaaccat atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt
     3541 ttttaaactt cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt
     3601 tttgctactt caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt
     3661 tcttggaaaa gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca
     3721 tccgctacga ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc
     3781 tgacctcccg aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga
     3841 gccagtcgat ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt
     3901 gctgctatga cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata
     3961 atcgagacat catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga
     4021 tagtatacct ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt
     4081 tttccagaac cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct
     4141 acatctttat aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt
     4201 aacatcaaat tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct
     4261 atagaagcta aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc
     4321 aattgtgcag gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga
     4381 ttattcgtgg ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc
     4441 atttgtccac cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt
     4501 agctgctgtc ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca
     4561 agaatcatac aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct
     4621 cctaaagata gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc
     4681 agagtaagta tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt
     4741 gccacgttgt ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta
     4801 aatgtatctg tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga
     4861 tcagatagag tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca
     4921 tttgcaaaag tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata
     4981 cccatactgc cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg
     5041 gtatcttcat cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt
     5101 tctgctagag tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct
     5161 tcaccatccg tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac
     5221 acaccactga tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca
     5281 ggttcaccgc ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg
     5341 gcagttattc catcttttgc catagctgag gcggaacttt caaaggaata actctttaca
     5401 ttatctaatt cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg
     5461 cctggatctg gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta
     5521 aatgaagctt ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga
     5581 ttcgctgctt cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg
     5641 gctgtgaaca cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat
     5701 gcacgtttca taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca
     5761 acctatctta aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg
     5821 tatatataaa aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt
     5881 tcaaaccgac gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag
     5941 ggatttttac gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt
     6001 tgtataataa tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca
     6061 aaagatccat tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac
     6121 gcggatgtgc tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca
     6181 tatgcttcat tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact
     6241 tggatgtcat aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc
     6301 gttcgtgaaa atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta
     6361 gattgtgggc taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat
     6421 tttacgtatt tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat
     6481 gcagatgcga tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg
     6541 acagagttga cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga
     6601 tttaaggatt tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg
     6661 agctttttcc gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa
     6721 gaaggtagta tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat
     6781 caaacagatt atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt
     6841 gaatcggcaa atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa
     6901 gtatttgata cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat
     6961 ttgcaacgag tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg
     7021 actggtcaag atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta
     7081 ccaagtgaag atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg
     7141 atcattttag aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac
     7201 ggtacacacc gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca
     7261 agcatcgcga tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga
     7321 acagtgaagt tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg
     7381 caattaatga tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt
     7441 gaattagctg ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat
     7501 attactggac gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact
     7561 acaagtgcag aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt
     7621 ttacgtgatc gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt
     7681 cggcgagaaa agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta
     7741 aaagctagcc gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa
     7801 catttagaaa gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta
     7861 ggccgttact tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa
     7921 gctactcaac gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt
     7981 gctcgtcttg ct
//
LOCUS       DASOUD010000102         7915 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.103, whole genome shotgun sequence.
ACCESSION   DASOUD010000102 DASOUD010000000
VERSION     DASOUD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.103"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(115..1602)
                     /locus_tag="U4A29_002230"
     CDS             complement(115..1602)
                     /locus_tag="U4A29_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681341.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
     gene            complement(2157..2720)
                     /locus_tag="U4A29_002231"
     CDS             complement(2157..2720)
                     /locus_tag="U4A29_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO0681342.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2735..3778)
                     /locus_tag="U4A29_002232"
     CDS             complement(2735..3778)
                     /locus_tag="U4A29_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681343.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3911..4315)
                     /locus_tag="U4A29_002233"
     CDS             complement(3911..4315)
                     /locus_tag="U4A29_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEO0681344.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4548..5183)
                     /locus_tag="U4A29_002234"
     CDS             complement(4548..5183)
                     /locus_tag="U4A29_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEO0681345.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5449..6501)
                     /locus_tag="U4A29_002235"
     CDS             complement(5449..6501)
                     /locus_tag="U4A29_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0681346.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6514..7452)
                     /locus_tag="U4A29_002236"
     CDS             complement(6514..7452)
                     /locus_tag="U4A29_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO0681347.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         2566 a         1595 c         1266 g         2488 t
ORIGIN      
        1 ataagaaaac taattaaaag aaagaggtaa ctacataaac taattaaaaa agctaacttc
       61 atagttggag gataaatgga agaaacttca atgattgttt ctccttgtgt tccttcaatc
      121 gacactagtc ctattatcaa tctctctttt ttgtccattt acttttgcga ctgttcccca
      181 ataatcaaat acaggaagcg ttattttttg tcctttagat tcatgtactg tatagactat
      241 tttcgaatcc atcacttgat aaggcatagt atgccattcc ttgccaattc ttacttcatg
      301 attctgcaaa tgttctttgt attgtaatgc ttccgtgggt gcatagtcat aaagtcctcc
      361 gggaaggagt gtcgtgtaac gaataagatt ttcttgattc attaccaagt gatctctctg
      421 agtgacaact ttatgttcat agtttgcttt tgttgcagca gatacagcca acagtaataa
      481 aacaattgcc gttatattaa ctacttttct ttgcttagct gatgtgctct tcatgaaaac
      541 gaacgataaa atcaacgagc caaacagtac ttgaaagcct aagattcgcc aaggaaattg
      601 aatcatttga atgggtgtat tttgtaataa agaccatgga aaaaatttgg tggataagag
      661 ccaagagact accgcactaa ggtaaatcac tttttctttg cgattaaatc ctttaaaaaa
      721 gaagagtgga cagatcaaag aaataatacc aatgattcct accgtaaagg ttgtcaggtc
      781 attattcatc gcaccaatca aactatccga gacatttaat gcttggtgtt gtaaggtcgt
      841 tttatctggc tcattaatcg attgaaccat gatttgtcgg aacatcggat accaaaagta
      901 tgctgttaaa agaatcgtca ttcctactgc ctttgacaac tccacaatca accggatgct
      961 ttcttttttt cttgtttcac ttactacaaa gccgcctaag actagtaagc caacggctat
     1021 tacactcata aaagtggaaa gaacatgtgt ataaatgagt aagctcatac tgactgctaa
     1081 tggaattgca catggctttt tctcataaat cagtgcgtaa gcataataca taactatcgg
     1141 tataaccgta agtgcaatcg cttcagcaat agcagaacga tgataaatat caatcgtacg
     1201 gtacccataa aatgtatata aacagctaaa tataaaagcc gcatcgattc tttttaaaaa
     1261 ttttagtcca taatgaaaca tcagcatgat agtacaaaca ttcaatagcc aaacatataa
     1321 gatataacta acgattaaat tattacttat ccaataaaaa atgactgccg gaaaaaaggt
     1381 caagtaggga taaaaataat tcactccgct cccataatga ttaaacgtcg taaaattgat
     1441 tggaccagaa aaaattgaag ataacccttt gactctagca aaatggaagg caatatcccc
     1501 cgatgtagaa atagtatgaa catttctaaa ataattgatt gaataaagtc cagcaataat
     1561 tgctaggaga atggacaata ttattttttt ctttacttgc attacttaat tatatactcc
     1621 tttcatggat tgctttatct agtataactt aaattttaaa taaatcgtta tttattttta
     1681 ctctcactta aaaaagcact aaaattataa gagagggagg taatggtctg aacaccaaga
     1741 attgttccaa catcgaattg cttaactaat gagttcaaag cttaattatg tggctcttat
     1801 atttaccgag cgtggattgc gatctcttta gcgaaacaaa catctttcat aaaaaaaaca
     1861 gccatatccg ctttcttgga tatgactatt tactactact ttactatctt tcattcttct
     1921 tgtctcactt atgataaggc gctgcgtatc atgagtgaat gatattagga aaacttcata
     1981 aaataaatct tttatacatt tccacttcta gatggtcatt catgttagaa gaatcaaact
     2041 atttttttac tgtcgtaata atattaaaac actagaacaa atcaattaaa ttccataaaa
     2101 tataaataat agacctagca tcatctggta taaaatggat gctaggtccg ttatatttac
     2161 tcaaaattga ttctacgaat agatcttctt atcatttcta ttttagtagt catgcgttgt
     2221 tgttcttcta aaggtggtaa tggtataagt agtttagata aatttgtacc atttacattt
     2281 ggttgcccag ttccgtaaga aattttttct aataacttcc aatataatgg cgagtctaag
     2341 aagcagtcta cgtattctga taataatttc tcaactaacc taattcgaat caagtaagaa
     2401 gcaaaaacag attcctcact tatttcctta accaagaaac tttttcccat agttcctcct
     2461 gtccttgcta tcaaaatgtc attttcttcc aatcttaaat caactagttt agaattacta
     2521 atatcacagt aaggcacact ggaccaattt acacgtccct cttgtatatc tgtgattctc
     2581 aaaaacttaa cattgccttg ttttttcgcc gaatctgtat agccatattg aatcttagta
     2641 gaaatacttc ctaaataaac ccatagtcga acatcgctag atgatttttc ttgtcgttct
     2701 ttatgaacaa cagtgatcat cttgagttgt ccgactattt tttagttgca gcaatctttt
     2761 tgggacgctt atttgcatga agcaacgttg ctaagtctag taataaaaca ttggctaaca
     2821 caggattggc tttctcgcga tacgctgcgg cgccactttc aaacaatact gctagacgat
     2881 cttctacaac aacgatttgt tccaaatacg gtggaaattt gattcgtttt aggatccggc
     2941 taggagagtc ccgctcggac actaagagtt ccgaggtata aggaccataa gaacgactgg
     3001 ctaagagata atggccataa aaagccacac cttgaatctt atcagatgtt tttctgctca
     3061 ttgtcacggt tggttggatt ttgtctttgc ggcgaattgc tgttttcatt tcagctatcg
     3121 tcggcaatag atagaaagtc aactgtcctt caccttttag tgtgaagttg ccagatacca
     3181 gttgatcatt gttttgagtc aaatacgatg cttgtggaat ctctgagaga tcagttgtca
     3241 gccaataagc gattggctgt cccgtctctt cagatgtatc tgctaagata tcgatcaaac
     3301 gtaaagcggc tactcgtcca gtagcagatc ctgtgattgt caaccataaa agttcccgtt
     3361 ttgtatcata tactaagccc cctacatgtg gccgtttagg taaaatcagc agtttgatcg
     3421 gctgtcctgt ttctctatcc agtacatgaa taatagaatg atgctgatgg tcatagcagt
     3481 aggcagaaat aaacacaaaa tcttgagaaa aagccaaccc ttgcggcgtc atccattgac
     3541 aaacctcatt ttcgccggtt tccggcacca ccattcttgt ttctgttaat ccaggaatgt
     3601 tatattgttt tgcttcagcc ttttcccaag ctgcttgaac aataggatac cgttttaact
     3661 ttttttcaat atcctgatgt atccgttgct gcttccaatg cttaaacttt aggacatagt
     3721 aaacaagaag gccaatgacc agcaacacta aaatcattat tgctacccgc tgtgtcattt
     3781 ttttgcctcc ttgttctatt cgatcaatcg tccatatacc gctattaatt accgtaacta
     3841 catgtttctt ttactagcgt ttctttatag gatctttcat aatctcattt atagtaacat
     3901 taagaatccc ttattctact atagtcactt ccacatcaat attgcctctt gttgctttag
     3961 agtatgggca aaatgcgtgg gcttcttctg cgattttctc tgcttggtcc ttctcaagtc
     4021 ctggcaaaga aacttctaaa acgaccccga tttggtagcc cccatcttct aattgaaata
     4081 aagatacccg tgctttgact tcagaatcag actcaagatt attttcttcc agcatgtgtt
     4141 gaaccgcgcc gttgaagcaa gatgcgtatc cagccgcaaa aagttgttcc ggatttgtcc
     4201 cttcactatg gattcctggg cggtcgatct tcatgtgcat acttccgttt ggtgcctcaa
     4261 cttcgccatc acggcctcca cgattgatca ttgctgtttc gtacattttt ttcattgaga
     4321 tcatcctttc tttcgtcgtt ctatattaag tgtaacgagt attttttaat ctgacaaagg
     4381 taatgcttgc attcggcgac atcagttaga caacatcgat gatcagtgat agaagcagtt
     4441 tatctataca aaaaaagaag ggttctatga tatttggtgt ccttactcgc cttgagtaac
     4501 aacaccaaat atcatagaac cacttaatca gcttcacact gctttttcta ctttcgtagt
     4561 tgaaaaaaac ttaaacaaat catcggcaaa tagttaaaga aaaaacttaa tcgttgaaag
     4621 aagcccaatt gcttaaagaa ggcaaactga ctaatcgacg gaatccgtgc taatccgtaa
     4681 agacctgcaa ttagtaaact aataactgtt aaaacaagat aaaaatggct aagcgtacct
     4741 tgaccacttt gacgataaag cagaacaagt aacaatggga aaagtagaaa gcctgcatag
     4801 cctagcccag aacctatatt atggatccaa gtcgatagat tacaagagct ttcattcgta
     4861 ttgatactga ataagccagt aaaaatacaa tcacctatcc catataaacc aactgttgca
     4921 acaacaagta ttgcaagtgt ttttgatgtt ccaacaaata ggtgatacaa cgcaggcaac
     4981 gaaagaacaa aaaaaagtcc agatacaact gaccagacca agaaagcacg tctgactgga
     5041 ctatctacgt caccaaacac actaattacg gtagtcattt gattcagttt gggataaaaa
     5101 aggccaagga tatacggtgt cgataaatca ctcatcacac ctaataataa aaaataaaaa
     5161 ccatattttt gcaaaaaaat cattttcttc ccctactttt tagtgattat tcgatacatc
     5221 caacaatcaa attttttctc attttaacac atatttttat tctagttgtc ttttgaacaa
     5281 tcaataacat aaaggtgagt ttcttctaca ttttaaaaaa tcgaatggca ctgaagagtt
     5341 cagttgtcag catcaatctc ctactcaaaa aggcaaaaaa acgtaagtca gatgttgttg
     5401 acatctcact tacgcatcca ttgattgaaa atttcttagt aaaatctact agcctaaaaa
     5461 tttgaagact tcttctcgat catacagttt aaatactttc tcgcataaaa attgagtaat
     5521 tagtgccgca aaaacaggga tgactaacca acaaagaaca acaagaaagc catttagccc
     5581 tgcatctaaa gaagccaacg gaccaacaag tccgaccacg ccgaaaccag cagaagcagg
     5641 cgtacctgaa atagaaaata gagcgacagg aatagctgta atcactgcac taactaaaca
     5701 aggaataaga atcacaggtt ttctaaatag attaggcatc atcatcttca tagcgcctaa
     5761 agcaatcgca attgtcacac ctgacttatt aaccttccaa gagttcacta ctaaaacaaa
     5821 agtggttgca gctacgccca tcgcagccgc accagcagaa acaccatcta gttggattgc
     5881 cattccgatt gctactgttg aaataggaga gataatcaaa aaggcaaagg aacaaccaat
     5941 caagatagac attaacagcg gttgtaaggt agtgaagtta ttaatcaatg atccaattgc
     6001 agaagtaatt gctgttacaa aagggaataa ataaaaacca agtaaacctg cacctacacc
     6061 tacaacaatc ggtgttaaca caatcgacac tgacccaaaa ctatttccaa tcagtaaaat
     6121 cagcccgaca gcaatcgatg ctgtcagcat cgtattaata atatcgcctg taccagcacc
     6181 aacgtaaaca cccgtttcac ccaaatcagg tgtgaatttg actacaccag aaccaacaaa
     6241 agaagctccg gctacaacca tcattctcat cggatcaaat ccaaattgta aggcaatcaa
     6301 accaccaatg atcaaaggtg ttgctagttg aaaaataact gctaactgtg cgatcgttac
     6361 cgcaaaggga tacgcactaa aatattttaa aatggcacct aatactgcat taggaatcaa
     6421 tccaataatg attcctgttg ctgtaccagc gaggacttta ttcagaaaaa ttttcggtga
     6481 tattttttgt gaagtttcca ttcaatctct cctttatttt acgttcaaac tatcttcttt
     6541 tgcatggaca agctcagtca agaaatcaca ataaggcgtc gcaacaccat atttcttccc
     6601 ttttctagaa attgcgccat tgatataatc gatttctgtt aatcgatggt tcttaatcaa
     6661 gtcttgatac attgaaggat aatgtaaccc aattgtttcc gggtcaaaac aagattcaca
     6721 atgtgcaatg acttctggga catctagttc aatcttctct actgctgcta ctttggcaaa
     6781 ctcattgaca atcgtcgcca ccattttatg agcagtcgat gtttttccta gctcagccat
     6841 attaacgtct aagattgtgc atagtccgtt cattgttccg ttcacacatg ctttgcgata
     6901 aatagaatag tgaatattgt cagaaaaatg agcatttaat cctgattcag acaatttgtc
     6961 tgctaatttt tttgcagctg cttcttttcc atcacctaaa ttttgtaatt ctactgaacc
     7021 acttccaaat aatttgacct gaccaggacc ttcaagacct gccgtccaca tggtatttcc
     7081 aatatagata ttttccattg gtacaaattt ctcaataata tcttcatgcc cgataccatt
     7141 taatagacat aagacctctg tatctttttt gattaatgat tggatatcct gcagcatttt
     7201 ttccagctgc atcgctttgg taaatagaat aatcagatca acttgatctt ctttttctac
     7261 ttcggattga agaacgattg gtagttttgc ttctacctct ttaccattaa aatttgcttg
     7321 taatccatgt tctttgattt gttggacatg ttctgcccag ccatcgatta ataatacttc
     7381 gttcccactt tggtgaagca tcaacccaaa gcgacttccc attgcgccag cgcctgcaat
     7441 tgctattttc attgattctt cctccttttt ctttctatta tactcatttg agaatctttt
     7501 cacaacctcg ttttgttatt cttctcttat ttatcatgtt tatatccacc attcaagtta
     7561 ttttatcagc aaatcaataa aaactggaat agaatcccaa tttcatcgta agagaaacta
     7621 gactagcaat aagaaaatag aaacattcat agaaaaaatc tcactcctct gattataaaa
     7681 gagggggcat taacctcgtg tatactaaaa ccttccattc attcgatgga tgattttctt
     7741 tttgtgaaaa aatattcatt gtagtacgat gttgcgcaac acagacggat ggtattgctt
     7801 attatttatt aaaatcgaac aatacactta tttaaaaact tctttcccag attttcttcc
     7861 atgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aagtc
//
LOCUS       DASOUD010000103         7781 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.104, whole genome shotgun sequence.
ACCESSION   DASOUD010000103 DASOUD010000000
VERSION     DASOUD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.104"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(82..588)
                     /locus_tag="U4A29_002237"
     CDS             complement(82..588)
                     /locus_tag="U4A29_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO0681348.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            748..1188
                     /locus_tag="U4A29_002238"
     CDS             748..1188
                     /locus_tag="U4A29_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO0681349.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(1214..2371)
                     /locus_tag="U4A29_002239"
     CDS             complement(1214..2371)
                     /locus_tag="U4A29_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0681350.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2374..2730)
                     /locus_tag="U4A29_002240"
     CDS             complement(2374..2730)
                     /locus_tag="U4A29_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681351.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(3028..4002)
                     /gene="sgrA"
                     /locus_tag="U4A29_002241"
     CDS             complement(3028..4002)
                     /gene="sgrA"
                     /locus_tag="U4A29_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEO0681352.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(4198..5037)
                     /locus_tag="U4A29_002242"
     CDS             complement(4198..5037)
                     /locus_tag="U4A29_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO0681353.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            5222..6109
                     /locus_tag="U4A29_002243"
     CDS             5222..6109
                     /locus_tag="U4A29_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEO0681354.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(6703..7398)
                     /locus_tag="U4A29_002244"
     CDS             complement(6703..7398)
                     /locus_tag="U4A29_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO0681355.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(7382..7780)
                     /locus_tag="U4A29_002245"
     CDS             complement(7382..7780)
                     /locus_tag="U4A29_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEO0681356.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2439 a         1534 c         1145 g         2663 t
ORIGIN      
        1 aggattattt tctcatttta cgtaataaag cagaatggat aaaccagtat ccattctgct
       61 ttttttaaac gaagaaacaa cttattgctc caaaaatgtc agaaatcgtt tatcccaaat
      121 tttctcgtaa aaagcgattg tttcctttgc tttcataaaa ggattatcat aagttgtcgt
      181 tgctcctcta cacatcataa gctggtatcc taatccatga gcaaacttca cagtcgtatc
      241 tatacaatac tctgcttgtg ccccacataa ttcgatcgaa tgacagtcca attcagttaa
      301 taaaacactc aaattagtct tataaaatga attcgcatgg gtttttctca caaaataact
      361 gtgctcagga atagataatt ccgacaatag ctcccatggt tttgtttgtg gcactaattg
      421 agcatcttcg tgttgaacga aaataactgg tttcccaatc gtttcgtatg ttttgattct
      481 tttattcact aatgctgtca ctgtttctaa ttcgtgtatc ggcgaagaat caaaacatac
      541 ccctttttgt aaatcgatca ctactattgc gtcggctgtg tttgacatac ttgtgccttc
      601 tttctttttt cttgatttaa tagtatttct tatctagcta gttccattat acgtgacaac
      661 attttcttat tttacacctc taaatggcac ttttcgacta tttcttcatg ttattatttc
      721 ataaatactt agctattgga ggaatgtatg atgattaaat tgtatacagg ttctggctgc
      781 tcgtcttcaa gacgcgcaaa acgttggctt atcgtccatg atataccgtt tatcgaacag
      841 aacttaattc gtcaaccact aaccaaaaat gaatttatcc acttactaaa attgacaaac
      901 aacggcgtga ccgaaatgct ctctactcat tctattagtt ataaaaatct tgttcacaga
      961 atagatgaac tcagtttgag tgaactatat attgagattc gtacatcccc ttctttattg
     1021 aaaaaaccga ttatctacga ccaatctgca aaacttttga ctggatttag cgaagataaa
     1081 atcaggatgt tcctttctaa aactaaaaga aagcaagagc tcaacgaact cttgctttct
     1141 ggtcatcttg tcgattttaa agaaaataat ctttttgcaa tgtcttaatt ttggcataaa
     1201 caatctgctt ttcttaatta acgaaatcca atttttccaa tatggatcta tttttctcat
     1261 tcctgatttt tatgatttca gctttcagac gcgcccaatc attttttgta ggttgtccat
     1321 tccagtaaaa tatctgtgta ttcgtttgat catatgcctt tttgtcgata ggataatcgc
     1381 taatgacaat atcaggtaac tgatcagcgg atgcactata ttttattggg cgttcagtca
     1441 tagataatat ccttttcttt gcccataatt cctgtgtttt accatacttt gattgaatat
     1501 agaatatgac aggttgttcg tgttttataa aaatatccct gactaacata cagtattgaa
     1561 aaatcaaccg tctgttcttt tcataataag tattaagctc tggaattctg cgagctaccc
     1621 cactaaaaaa ctgttgcatg attggataca tcactggaaa tatatgctga aaatctctgt
     1681 cttctgctga ttttccaaaa gcatccacct tcttttttgt cccaaaaacc cttgaataca
     1741 aatggatcat tgccaaattg acatatagat aatttttttc tgaagttgta aaacaaatct
     1801 tgaagttttt ttcaaaattt tctacaatag acgattcaat ccagccccaa ctttcttgta
     1861 ctttatctgg agtatccaac aaatcttctt ccaaataggt gttcattaca ccaaacataa
     1921 aataaaggta agcttgctct gttggagtga tgtgattcat cgaccagaag tttgcttcaa
     1981 aaaaaggctc tagtatgtaa tgcgtaaact cttcttttgt tatcaatgga tgcacaaatt
     2041 tgttcatctc ttctgggaca tacgtaatcg agtggccttg tttcattcgt tgcaacgaaa
     2101 ttcttagtag gctgatccat ttctttctat ctatactacg cgtatatggc gcatgtttcg
     2161 caaatttttc taatagttga cgtttatttt ttatacttga taatatatca gaatgatttt
     2221 cgtcgaatga ctgccaatac atcaagtgaa aaaagtatcg gatacggtat tcttctcctt
     2281 ctatcggatt accttttttc aagctgcaag ataaatgata gtttttcaaa atagtattaa
     2341 gttctcttaa ttttctttga atcgtacgca aacttatata attttttgac taagattttc
     2401 taaggattga taagtacctt tgtaaagtga atccagtaaa atataaccaa tcgttccctc
     2461 taaaagtata gaataaactt tactcagtaa aaactgagaa gaaaactcga cttttatcat
     2521 cttgttttct gaaagaaaag ataatttata ttctggatac ctttcgcaaa acgattctgc
     2581 ttgttgaatc accgaattca tcttagccag ggtgtagttt agatctaaag cgatatcctt
     2641 caccacatat aatttctcgt ctctttcata acaatagttc aaaaattgat atacttgcct
     2701 ttcattgata tcaataaatt cttcaaacaa agtacatact cctttttatc tattatttag
     2761 acttattccg acagctacat gaataacgta caaatagatt cgttactgat ttttcagaga
     2821 tactaccatg cgcagtgtgc attgtcaatt attcttgctc ttattttcca tttgtgttcc
     2881 tgttatttcc tgtttctcaa atatgttagt aaacaaaaat agatctggca aagctacaac
     2941 ttcgccaggc aatacagcac tctcgaatga aatattcact caaaaatcta tcgttttatt
     3001 caattttaga tctgttgctt ttattcttta tttcactcga tggaagagag aacgaaaata
     3061 gcttgctcct acaatcaaaa tgagtaacac acccatccat gtaattagct tgttcaaggt
     3121 tctactacca gtttctggta aatcgctttt tctattatct gtttctttcg ctttttcttg
     3181 atgatcaatt ccacgagaaa caaaatcagg ctgttttagc acacttttta ggttgctccc
     3241 tgcatttctc ttttgttcct tagttggtat gataggtacc aaatcaagtt cattatttac
     3301 ttttcctgtg ctgctttcgc tgctcgtgct tcctgtgcta ctctcactac tcatgcttcc
     3361 tgtgctactt tcactactcg tactctccgt actgctttcg ctgctcgtgc ttcctgtgct
     3421 actttcacta ctcgtactct ccgtactgct ttcgctgctc gtgttttctg tgctactttc
     3481 actgctcgta ctctctgtac tgctttcacc gctcgtgttt tctgtactgc tttcactact
     3541 tgtgctatcc gtactgcttg gcggtccaac aactgtccaa ctgcttggtt tcaaattctc
     3601 cgtgatcgtc atctgtccat cataaaaaac aatcaaatca actttatctt tactcttgat
     3661 ctcatcaatc gatagttctg cagaaatcat tattgtatac tcaataccag cggtccattg
     3721 tacgtctttt aaataaactt ccccaataac tttttctgaa cttccattac catcccaacc
     3781 atatgttaga acagcatgta catcactagc attcacgctt ttgtttggtt tgactggaat
     3841 agtaatgact tgttgtgtca tgtcataaac aatccgatca gttttcacag taaatgaaag
     3901 attctcattt gcccgttcat ttgcagaagt actgattcca atcattgctg caattatact
     3961 cagcccaaga ataacaaata atacggtcgc tgttttcttc atttctatca ctctccatat
     4021 aattaccttc tatttgataa aggaattata taaaatgaaa atgatgagaa cacgacagtc
     4081 agttgtgaaa atctaacaaa agatgtcgtg tattcgcttg aatttgttta ctttttcgtt
     4141 taatcaatga cagacttaaa aaagcaagac cgatatcagc cttgctttct tcatctttta
     4201 ttgataaaca gcagtgtatt tcttcatccg ctttgtttta tatgcataga gtaacaaacc
     4261 aacaatcaag actaacaaga ataatgtagt tgatatagat gcttcagccc caaagtttcc
     4321 cccagtaatt attgtatttt gcgattggat cagagtggag aaaaaacctt gttcttgcgt
     4381 tccgctgact ggtataccca aaatagctcc ttgtgtccaa ttccaagtaa tatgagcacc
     4441 aatagccgta aataaacttc ctgtactgta gataagtgcg ctgaaaaagc aacctgctaa
     4501 aaagatattg aaaactgcca gaaatgtcat gttaggattc aatccgtgca aaagagcaaa
     4561 taatgcagca gatccaatca ttgctattaa cggaccaaat ctaagcatct gatgctgaaa
     4621 aaagccacgg aataaaagct cttctgatgc ccccactgca ataaataaaa taaaactcgt
     4681 tatcagcggc acaatgtttg cgtttcctga atctaaccga tacgcccccg tcaaatataa
     4741 tggactagta ataagcagaa acactccaat tccaacaacg agacctcccg ctaaataccc
     4801 ccagtctttt cctcgaacag aaacaccata atcttctatt ctttttcctt cgaccttact
     4861 agcaagaaat aaagctagta aaaaaacagc taaatgtccg accgtaccgg caattgctcc
     4921 aatccattca ctatttccaa taaacatatc cgcaagtgca ttattgacaa gaattcctaa
     4981 aacaaaacca actaagacta ttaaaacaga tccaatcaac cgaataatcc tgatcatctt
     5041 tcatccctcc tttatattaa gtttaacaac ccttttcttt cttgtctcgt gaaaactatg
     5101 taaatgttct ccatcataga gagaatagat ttttacgaac taagaatatc tacagcaacc
     5161 ttacgaaaat gaaagactgt ctccctaatt cttctgttag actgttttta ataaggaaag
     5221 gatgggaaat tttttgaaaa aacaactact aattatgtgc ctttcagcat gtgcaatttt
     5281 actattctca ggttgtagca agtccaatga aacgattgca acgtttaaag gtggaacact
     5341 tacagttcag gatttttatg ataacccaaa agttaaatca aataatcaaa cactattaaa
     5401 aaaaatgatt gtcttcaaag catttgatga tgtatacgga aaagagattt cttcaaagga
     5461 agtaacaaaa gaatataatg aacaaatcaa aaaactcggt ccaaactaca aggaacaatt
     5521 aaaagcagtt ggccaaacag aagagacata taagaaactc tttaaacaga tgctggcttt
     5581 ccaatacggt ttgaaagcca atgtaaaatt aacagataaa gatctagaca ctgcttggaa
     5641 agaattctat ccagaagtta gtacacagat cattctattt tcgactaaag aagaagcaga
     5701 taaagcaaaa aaagaagcaa atgaaggaga aaatttctcc aagcttgttc aagcttatgg
     5761 aaaaaataaa acattaaagg aaactgatgg aaaaatgaat tttgattcaa ccaatccaga
     5821 gattcctact gaagtaaaaa aagcagcatt taaactaaaa aacggggaag tttctgatat
     5881 cattcctgta acagatccaa cgacttatca gcaaagctat tatctagtaa aaatggtaaa
     5941 aaaacaggat aaaggttcta ataaagacaa atacaaatct gagctagaaa aaattgctac
     6001 cgaagcaaaa actcttgata cggaattcat ggataaaaca atccgtaaag tgatgaaaaa
     6061 agacaatgta actattaaag atccttatgt aaaaaatata ttcaaataaa caaatttgac
     6121 ccaattgggg atgtggcaaa atttgagaag tgatttttgg aaattccttc ttatttttga
     6181 ggcaaaccac ttttgccaca acttcatcgg ggcctttttt tatttgtacc aaaacaaatc
     6241 actggatttg ttacaatcat gaggatataa tttaaaaaag ttttttattt ctatcacatg
     6301 aatcaatgcg ccattaagta gattctttca tgcttcccta ttttttcact tgccgtatcg
     6361 cttcaaaaac aactatcata aactcaaact cgactgaaaa gttattttaa aaaagactaa
     6421 aaatatccct tctggacttg ttcttcttat tctgttatac tagagaaaca cgaaagatat
     6481 ttttatgcga cacttttcat ttgtttttat gaaatttatt caaaactggc gtaacttcag
     6541 agttacaagg atgtattaga ggttagggaa tacatcgttt aaatgagaaa taagtaatag
     6601 ttagtaatag tcacccaaac tttaaattaa tgaatattga atcgtcagat agcctcctcg
     6661 agttttcgag aaggctatcg tttattttat ggagaaattg attcagaagt aatcagcgac
     6721 cttttttagt tcaccaatcg tttctgcttg gaaagtttgc acccaatctt cttggaagcc
     6781 cctcactgct ttatcaatcg agggcatctc taatacttga tctagtagct ctttacctaa
     6841 cgttatcgca tgagctccag atcgaatggc tgtagtgact tgttcacaat ttttgaaact
     6901 agctgctaat atttttgttg gtctttgcgt acgttcgatc tcagtccgca acgaatcaat
     6961 cactttcata gcatcaatgc cttgattttg catcctgtta taataaggtg caatataatc
     7021 tgctccatta ttaatagcta aatacccttg taattctgtg taaatagctg taccggtaat
     7081 caaataacct tcttttttca actttttgat cgcttgaagg cctgcttgat tcaccggcac
     7141 cttgataaac gtatcttttc ccagttcttc tatgataact cgagcatcct ctatcatttc
     7201 ttcttccgtt cttcctattg tctgaatatg gataggaatc tgtccgatta tttttttgat
     7261 ttctttcata tgcgagaaga agtttgacgc tccttctttt ttcagaattg taggatttgt
     7321 tgttacgcct gttaaattca aaaactgttg acaagaataa atactttttg tatttgctgt
     7381 atcaagcatg atttccatat agctttcccc cattctgatc ctcttgatcg attttaatga
     7441 ttgtcccctt tcttaaatgc ggcatttcct tatcttctac atacattgtt cctggaagtt
     7501 ccggctcatg agatccatca aattttatcg tgatatgtcc taattcattc aaattagttt
     7561 ggacaacatc tcctactgct gtgattacat acatttgttt atcaaaacag agcttcatat
     7621 cacaagttat ttcttttctt acctgattca attcaatctt ataacaataa tctgttaatg
     7681 tttccggtgc ttcatctcca aataaaatca ttattttttc tttcctgaat acttctgctt
     7741 ctataccaac atctgttaca accgtttcaa agatcgtcat c
//
LOCUS       DASOUD010000104         7760 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.105, whole genome shotgun sequence.
ACCESSION   DASOUD010000104 DASOUD010000000
VERSION     DASOUD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.105"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="U4A29_002246"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="U4A29_002246"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO0681357.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="U4A29_002247"
     CDS             complement(2032..3285)
                     /locus_tag="U4A29_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO0681358.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="U4A29_002248"
     CDS             complement(3291..4214)
                     /locus_tag="U4A29_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO0681359.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="U4A29_002249"
     CDS             complement(4435..6927)
                     /locus_tag="U4A29_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO0681360.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="U4A29_002250"
     CDS             complement(6934..7401)
                     /locus_tag="U4A29_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO0681361.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="U4A29_002251"
     tRNA            complement(7537..7610)
                     /locus_tag="U4A29_002251"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="U4A29_002252"
     tRNA            complement(7668..7741)
                     /locus_tag="U4A29_002252"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DASOUD010000105         7634 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.106, whole genome shotgun sequence.
ACCESSION   DASOUD010000105 DASOUD010000000
VERSION     DASOUD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.106"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="U4A29_002253"
     CDS             complement(105..629)
                     /locus_tag="U4A29_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0681362.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4A29_002254"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4A29_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO0681363.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4A29_002255"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4A29_002255"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO0681364.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="U4A29_002256"
     CDS             complement(2351..3031)
                     /locus_tag="U4A29_002256"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO0681365.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="U4A29_002257"
     CDS             3157..4026
                     /locus_tag="U4A29_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO0681366.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="U4A29_002258"
     CDS             4052..4567
                     /locus_tag="U4A29_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681367.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="U4A29_002259"
     CDS             complement(4614..5207)
                     /locus_tag="U4A29_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0681368.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="U4A29_002260"
     CDS             complement(5232..5411)
                     /locus_tag="U4A29_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681369.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="U4A29_002261"
     CDS             complement(5488..5997)
                     /locus_tag="U4A29_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681370.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="U4A29_002262"
     CDS             complement(6093..6473)
                     /locus_tag="U4A29_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO0681371.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="U4A29_002263"
     CDS             6803..7552
                     /locus_tag="U4A29_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEO0681372.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2173 a         1583 c         1356 g         2522 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aact
//
LOCUS       DASOUD010000106         7597 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.107, whole genome shotgun sequence.
ACCESSION   DASOUD010000106 DASOUD010000000
VERSION     DASOUD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.107"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(940..1353)
                     /locus_tag="U4A29_002264"
     CDS             complement(940..1353)
                     /locus_tag="U4A29_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEO0681373.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="U4A29_002265"
     CDS             complement(1809..2294)
                     /locus_tag="U4A29_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681374.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="U4A29_002266"
     CDS             complement(2433..2648)
                     /locus_tag="U4A29_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEO0681375.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="U4A29_002267"
     CDS             2872..3672
                     /locus_tag="U4A29_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO0681376.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="U4A29_002268"
     CDS             3691..5559
                     /locus_tag="U4A29_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEO0681377.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPTNRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="U4A29_002269"
     CDS             5552..6043
                     /locus_tag="U4A29_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEO0681378.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="U4A29_002270"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="U4A29_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEO0681379.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="U4A29_002271"
     CDS             6603..>7597
                     /locus_tag="U4A29_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEO0681380.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1443 g         2233 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc accaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DASOUD010000107         7554 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.108, whole genome shotgun sequence.
ACCESSION   DASOUD010000107 DASOUD010000000
VERSION     DASOUD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.108"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..1212)
                     /locus_tag="U4A29_002272"
                     /pseudo
     CDS             complement(1..1212)
                     /locus_tag="U4A29_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1487..1984
                     /gene="dfrG"
                     /locus_tag="U4A29_002273"
     CDS             1487..1984
                     /gene="dfrG"
                     /locus_tag="U4A29_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEO0681381.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2056..4008)
                     /locus_tag="U4A29_002274"
     CDS             complement(2056..4008)
                     /locus_tag="U4A29_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEO0681382.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4015..4251)
                     /locus_tag="U4A29_002275"
     CDS             complement(4015..4251)
                     /locus_tag="U4A29_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681383.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(4850..6718)
                     /locus_tag="U4A29_002276"
     CDS             complement(4850..6718)
                     /locus_tag="U4A29_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681384.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            6684..>7554
                     /locus_tag="U4A29_002277"
     CDS             6684..>7554
                     /locus_tag="U4A29_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681385.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFI"
BASE COUNT         2452 a         1261 c         1163 g         2678 t
ORIGIN      
        1 aagataagaa ataatagtcg ttactgtata acttgtgctt actttaatat cagcaactcc
       61 tttgactccg cttatttgta ccaatccatt agcattcacc tgctccttta caggttgatg
      121 atttatttct acattcgttc ctaaataagc atatacagga agtgtgatta cttggctctt
      181 ctcattagtt actctatata tgatagaact accagttgtt ttataaggaa ggtttaccca
      241 ttgactgcca atctttgctt cgtgttttat tatatgctcc ttattctcca atgaagcgat
      301 aggagaataa tcatataaaa aataatccat tcctttagtg gtatacgcct ctactgtttc
      361 tttgtttaca acaatatgtc ccggctgaga aattattttt tgattgaaat tttttttact
      421 agaaacagtt aaagtgatta ataacaaaac agtcataaat aaataaaata gttctcttgt
      481 cttattgtat cttctatttt tgaaaaatat catcgttaat atcagagaag agaatattat
      541 ttgcaagctt aagatccgcc aaggaaattg taacaatttg gctggcgtat tttgcagaag
      601 ataccatggc accaaactag ttgtagctag ccaagtcaaa cacgtaccat aataaatctt
      661 tttttctatg tttgttaatt ttttaaataa aatcagggga atacataaag ataacagacc
      721 aactagtccc attgagtaag tagttaagtc gttgttcata gcttcagtca gactatcgaa
      781 cacattagat gcatgagcat ataaatttgt cacgctaggt ttgttgattt tttggtagag
      841 catttgttca aacatcggat accaaaaagc actcgtcaga accaatgtca ttcctgccgc
      901 actaaataat tttttaaata tagcaataaa atctgcattc ttgaaacctt tagaaaccaa
      961 acgaataaaa ataaaaatga taattaatgc tgtactcatt aatgttgata gtacatggct
     1021 gtacactagc aaactcatac tcaatgctag ctttacagac ggatactttt cttcttttcc
     1081 agagatgatt tgataagcat aaaataatac agggataaca agtgttattg cgatagcttc
     1141 ggcaattgcg gaacgatagt agatgtctac tgtccgataa gcagagaaaa tatagagaca
     1201 actaaataaa aataagtagt tcagttttgg agtacaaaat gacaaaatgt acttcaaaat
     1261 ggactacttt tttcatttta acttacatac caaattacat acaaaatgat ttataaaatg
     1321 caattgaaaa tgataattga atgaaaattt gtgattttgt ggtattatga taatataact
     1381 tatagaaaaa attgcttatt caactaacgg gcaggataat gagaaaaggt tttttgatag
     1441 aaataagaaa ttagatggta aattagttaa aaaggctgtg ttataaatga aagtttcttt
     1501 gattgctgcg atggataaga atagagtgat tggcaaagag aatgacattc cttggaggat
     1561 tcccaaggac tgggaatatg ttaaaaatac tacaaaggga catccgataa tattaggtag
     1621 gaagaacctt gaatcaatcg gaagagcctt acctgacaga agaaatatta ttctgacgag
     1681 agataagggg tttaccttta atggttgtga aattgttcat tcaatagaag atgtttttga
     1741 gttatgtaaa aacgaagaag aaatttttat tttcggagga gaacagattt ataatttgtt
     1801 tttcccttat gttgagaaaa tgtacatcac aaaaatacat catgaattcg aaggagatac
     1861 tttttttcca gaagtgaatt atgaggaatg gaatgaggta tttgcccaaa aagggataaa
     1921 gaatgataaa aatccgtata actactattt tcatgtatat gaaagaaaaa acttattgag
     1981 ttaacgggtg cgttaattga agaagaacag taaagataaa gcacaagagc ttgggattac
     2041 tccaagctct ttttgtcatt tgaaataaat tatatctttc caatcaaatt gtttatctgt
     2101 aatccctaat ctttgagcag gtgtcaaaac ctttttatcc gctgatttaa aagggcgaca
     2161 aaagttgtag taagttctta gaattgtaat cgcaaattga gcatacttcg gattgaagtt
     2221 agcgtaaata tagctcttct tatcaccacg agctgttgtt aatggtcttt caagagatga
     2281 tatataacgt cttatctgtt gaataaagct attggtcgaa tggtcattaa catttaatag
     2341 catctttgcg atttcgtttg gttccagagc agataaatcc gttctacaat caactgaata
     2401 aaatccttta tccttagtgg ctaatggatg tttgataggg ttatcggcat atttgcgata
     2461 tctttctcca tctttactaa tagcctcttc gtggaattga tgacttgtta gtaattcagt
     2521 tagataatga gatgccaaag tccgtagaaa ctttgttttg aatcctctga tatctcccca
     2581 atcgagcagt tctgcttttg cttgtccgaa ttcttttaag cattgttttc tgcttttcgt
     2641 cttgttaact tggcaaataa aatgatgtgc atcgctcaac cttaattctt tcgtgaatac
     2701 tctataaaag gcgtttctaa tagagaagtc atcatcacta atcattcgcc attcgtcggc
     2761 attaaccatt tgtttaatta accaatagtg agcagttgta gtataaggag catcaacatt
     2821 taaaccatcg acaaatctac tcctattagt aattttccct aattcgtgaa gataagcatt
     2881 cctattttcg ctatcgttag cggatggttc ttgtggataa taagaccaat ctaacctatc
     2941 atttttacga ctaaaagtat tgagatggtc ttctttgaat tttcttgtat cttcatttaa
     3001 ttcatccatt gaaatattcc aatcataggc aacatcagaa cggaacacat atcttgataa
     3061 gacctcagca gatacaacta tatacgtttg catattaagg tcttcaaacc ccgtatattt
     3121 ctttgaacct tgtcctttct tacggacgtt atttaggtag tagtgcatct tgtccgtatt
     3181 aagccacatc tcgttaaatt tctttgtttg aaggggttgt gtctcatatc gttctaaaaa
     3241 ctccaaacaa cgcctataca gccactccag cttatgatag taggttccaa ctccaattcc
     3301 taaaatatca caagtacgac tgactgaaac ccttccaaca accatcttag cgaacattgg
     3361 aagaatggtg ttcttttgtt ggtgataagt agtggtttct tctctttttg gtagaacatt
     3421 agtgaacttc ttacacgcct tacattgata gcgttgagat ttgcccctac ttttccccct
     3481 tttgtaaaat tgcttcggct cattgaatgg ggttgattct tcttccgagc agccttcttt
     3541 atgaaattga taatctggct caacatcttg gatgctattg atttctataa ggcgtttaat
     3601 ttcttcaaca acagaccaat tagataaagg ggttactgta cagttttgcg atacacctct
     3661 gccaatagga ttgtcattgc agtacatacc tttatgacct ttatctttgg aactaccagt
     3721 cattttatat cgacttggtt tcccttttac atctttgaat ttgtgctgtt ctttcccaaa
     3781 attacaacaa aacggattga tgcagtaatt atattgaatg ggatattcag aaccgttcca
     3841 tttaaaagac gttggcaaga acaacatttt gttatacctt gttgcaaatc gttttggagt
     3901 taataaatca tattgtttag ccctatcttt taattcttcg tttgaaatcg gaattttaac
     3961 atctacaatt tcatcttcct tcgtggctaa tcttttaagc ttggtcattg tctatcaacc
     4021 cactctatct ttgataccca ttttttcaac aatccgctta ttacttcgct tgttggcaat
     4081 ccattcaata aacttcttat cgtctaaaat gttcaataag aattcatcca ctgcttcgct
     4141 ttcagtacgc tctgcatatt cagcgtattg cttaactatc acccttactt gctctgatat
     4201 ttcccaatcc acagacttag cgttcttatt ttttggctca agaaattgca taggaaaatg
     4261 cacccctttt tgcttagtaa attgtaacca ttttaacaca ccaatttgca ttcctacaat
     4321 aattatttca cgttcgcatt tttatataca ttttaactta cattttgtgg tgtaaattaa
     4381 caacaaacgt tgatttaaca ataaaaaacg agtatcaaat tcagttgcaa tttgatactc
     4441 atttaggttt acattttcac tttttgtact ccaaaactga actacttaaa ataaaaatgc
     4501 cgcttttttt tgttttaaaa accgctcgcc ataatgataa gctacgagtg ttgtacacaa
     4561 gttcaataac caaacgtaaa taatatagct tacaatcaaa ttttttgtca tccaatataa
     4621 cattacagct ggaaaaaaag tcagaaaggg ataaaaataa tttacaccat atccagtata
     4681 attgaatgtc tcataattaa tcggactggt aaaaatagtc gataacccct tgattcgaga
     4741 taagtgaaaa gaaagatctc cagtggtata aatactctgt gtatttttga aaaagatgaa
     4801 agaatatata ctactaacta taattaatcc actcaatatt actacttttt tatttatttt
     4861 cattcttctt gtttatctcc tttttagtca catatcgaga atctttcaac ttattaggtg
     4921 caaatcgttt aaaacttgaa tacaccacta aacaataagt cagaatactg atccataaag
     4981 ccatgagagc agttggagta tattttgttg taatttctat cttattttct ccagcattta
     5041 catccacatt cactaagcca tgttttttat aagagtcatt tacgacttta ccgtttactt
     5101 ttacctgtgt acctaaataa gcatatactg gtactgtcac tttttgcttt ttgttgcttg
     5161 ccactgtgta agtaatgtta ttatctgcag ctttatactt ccctttttcc cacgattctc
     5221 ctacttttac ttcatgtttt tctaagtgag gcctttcttt tatcgcttct gttggggcgt
     5281 aatcgtataa tcctcccata tctgatgttg tataacgtgg gagtgtttct tcattgataa
     5341 caatatgacc gtgttttgat tggataactt ttgcgtaatt ttcttttgct gctatagacg
     5401 tgacgagtag aaacaccaca gtagcgccaa tgattaacca tgaatatttt ttattcatag
     5461 tctttttcat aaaaacaagc gttatgatca ttgaagaaaa aaagatttgg atacctaaga
     5521 tacgccaagg aaactggagt aagcttatcg gtgttttttg aaacaaaaac catggaaata
     5581 aattagttac gcatacccat gtagaaacta cgccaacata gatgattttt tcttttttct
     5641 ccatttcttt cagtatgaat aaaggaaaaa tcaacgcaac caatcctaat aaccccatag
     5701 tgtatgttgt aatatcgtta tttaacgctt gaatgatact atctgaaata gaaagtgctt
     5761 ctagctgcaa atctgttttg aagggactgt gtatttcttg ataattcatc tgctgcaaca
     5821 taggatacca aaaatagcta gttaatgcac aagtcaaaaa aacagccttt actaaattca
     5881 cgaccacaaa ttttatatcg ttttttattt gcttgcttaa aaacaaacga actataatca
     5941 tacaagcaat caatgcacta cacaatagtg tagttaagac atgagtatac acaagtagac
     6001 ttatactgac agataaagcg atgacttttt tttctctatt gaatatcaat ccataagcat
     6061 aatataaaac agctgggata atcgtaagag cgattgcttc tgcaattgcg gatcgataat
     6121 agatatcaac tgttcgataa gttgcaaaag tatataaaca actaaataaa aaagcagcat
     6181 ctatcttttt aaaaaacttt tctccataat aaaaactaat cagaatcgta caaatattta
     6241 ataaccaaat atatagaaca taacttaata ttaagttatt tgaaattcca taaaaaacga
     6301 ctgcaggaat tattgtaaga aaaggataaa aatagttcag tccatctcca taattgttaa
     6361 atgttgtata gttgattggc ccttcaaaaa tagaagataa ccctttgatt cgtgaaagat
     6421 gaaaagataa atcacctgtt gtataaattt cttgagcatt tctcataaaa tttactgaat
     6481 ataaagtact aagtaagata agaactacta aaagaaatgc ttttttactt attttcatag
     6541 atttgttgtt ctcctttttt aattctattt tcagtatact agaattgtct ttttttaata
     6601 agttttgcgt taaatttttc atagcactag tatgatatac tagaaaagaa tcaattttta
     6661 ttacttttaa ataggagaaa aaaatgaaaa aaaacaaaaa ccaaataata gatatcattt
     6721 ttatgttatt tctaagtttt gcttacgtca tccctctatt catgtctaag ggaatctatc
     6781 atagtgtcaa tcaggatact tactttcatt tatctcgaat cataggacta gataatgtat
     6841 ggagttctcc agtaaacttc aataacttcg atcatcatgg aacaatgatg aatatttttt
     6901 atccatggct tactctttat ccagcttttt tattttataa aatgatggga aatcttgttt
     6961 taagttataa tatctactat ttttttatca cttttttaac aatggtcgtt tcttattttt
     7021 ctatgaaaca aataaaaaac aatcgttata tttcgctgtt attttcgatt atttatacat
     7081 tttccgccta tcgtgccatt gacatattcc atcgtgcttc attgggagaa gctgtcgctt
     7141 taacattttt gcctttgata ctaatggggt gttacgaaat ttatattaga gattaccaaa
     7201 aatggtactg gttgtctatt ggcatgacgc tagtagtgta tactcattta ctttctgtcg
     7261 ctatggtttc tgtatttatt ggaggaacgt tgtttctttc tttttatttt tgggaccaaa
     7321 aaatcgctag actcttaagt cttttaaaag ctaccgtcct cacctttttt ttaagtgcgg
     7381 gattcttaat tccttttatt caacaatcta gagcacaaga attgaaagtc ccactgggaa
     7441 aagaattaag tggtatggct cctagtgata tgctgacaca tattttaaat aataactata
     7501 acaactatac tattggatta ttcttatttc tcggattgat tggtgctttt attt
//
LOCUS       DASOUD010000108         7347 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.109, whole genome shotgun sequence.
ACCESSION   DASOUD010000108 DASOUD010000000
VERSION     DASOUD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.109"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            81..1079
                     /locus_tag="U4A29_002278"
     CDS             81..1079
                     /locus_tag="U4A29_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681386.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            1092..3110
                     /locus_tag="U4A29_002279"
     CDS             1092..3110
                     /locus_tag="U4A29_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEO0681387.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(3170..3886)
                     /locus_tag="U4A29_002280"
                     /pseudo
     CDS             complement(3170..3886)
                     /locus_tag="U4A29_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
     gene            complement(4113..4655)
                     /locus_tag="U4A29_002281"
     CDS             complement(4113..4655)
                     /locus_tag="U4A29_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO0681388.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(4660..4887)
                     /locus_tag="U4A29_002282"
     CDS             complement(4660..4887)
                     /locus_tag="U4A29_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO0681389.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(4966..6846)
                     /locus_tag="U4A29_002283"
     CDS             complement(4966..6846)
                     /locus_tag="U4A29_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEO0681390.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(7035..>7347)
                     /locus_tag="U4A29_002284"
     CDS             complement(7035..>7347)
                     /locus_tag="U4A29_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0681391.1"
                     /translation="KGAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLL
                     TEFEKREKGKEIFLFTDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKII
                     Q"
BASE COUNT         2163 a         1442 c         1230 g         2512 t
ORIGIN      
        1 ttttctgtct acacgttgtt ttttgagata ttctaagtta ggatgttatt aatgggcaac
       61 ttatcaggaa acgttttctt atgtttttgt ccaatttttt tgtgaggtgt ttattcttga
      121 aaaaaataaa gaaggagagt tatggcttcc tgacaatctt tgtcgcttgt ataatgatgg
      181 ttttacagcg tatattgatt tcagcagtca ctattcctac atatgcaaca aaccctggtc
      241 gattctacat ctatacattt ttacaaactt ccgttgttat tggtgcaaat ttgatcccac
      301 tttacatggg ttaccaatat caaaaaatcg cccgtttaaa agttttacat tatttaagtc
      361 gttttgcctt tatattccta atagccacac tagtttgcaa tatctttttt tacgtacaag
      421 ctggcagctt gaatattcgt gattattggc ttatcctaac acctatcagt cagaattact
      481 ttacttatgc tgtttcttgt actttgcttt tttgtagtat tccttataca gttggatttt
      541 taaataaatt atcgaaaaca acaattaaat atctactatt atttttaaca attggattag
      601 ttggctgctc tactctattt aataaagacc cgtttgcatt acagaatgga cagagtattt
      661 tatggatttt ttatttattt ttaatgggtt atggtttgaa agaatggaac tggaaagaaa
      721 aggtccgcta taagcttcct caagtattta tcagcttgat tgttttattc ggtttgatta
      781 ttttgatgac acaaatttca ctcatcatca gaggaaatac agatacagcg ctgaggtttt
      841 ctaaaccata ttctctcttc gcaatgtatt atactttctc ttcgttttta tttttagaaa
      901 tgctatctga taaattaggt ataaaaggaa gaggaaattt tcttgctgta tttttgattg
      961 tcactcaagt tatatgtaat ttccctttag tttcttatcg cgtatctaca tttatgaaac
     1021 gtgaatttcc aaactcaggg aaggcatggc tgataaatat cgggcagttt tttatttgat
     1081 atttattagc agtgtccctc ctagttatct ttttatttta tatacaaaaa atccgtttca
     1141 ttcatcgttt catcgaatac ttcgcttttg attcgattga acaattaact cagcgttgga
     1201 aaaaaagaat tcattggctt tttgttcata aaagttattt tttagttgca gcattttttt
     1261 actgctttac cttcgtgcag attttcgttt tagaaccaaa agaaggttgg aaagaaacta
     1321 tccaggttgc cttaaaaata tttactcagc gacaagcacc catgatcctg acaatcatca
     1381 ttattatggg atttttcttc ttactattgt tgatttccaa tcgtttttgg tacgccttga
     1441 ctgctacctt gatcattaac ttactgctca ctatttcgac agtaatcaaa atggaaatgc
     1501 gggaagagcc tgtctttcct tcagatttaa aaatgctgac cggattgtca gaactattat
     1561 cgatggttag cccagtctta ttaatcgtgg gcggattgat ccttctccta ttattgatta
     1621 ctagtattat tgtacaacgt agactacaaa aacagtattc cttgaagatt cattggaaac
     1681 gtcgatttat cagtatcgct gtattattag gatgtttttc gggtgtcttt ttcattaatc
     1741 acaaaaattc gccttctttc ttgctgttta atttatttaa agtaaacaag actttcttta
     1801 accagcaagg cgccgtaaaa gataacggac cagtcattca attcctaaac aatatcgaca
     1861 ttaaaatcat ggaagaacct gctggctatt cgaaagaaaa aattaatgga ataatgaaaa
     1921 agtacaacac aaaggcagat gaaatcaacc aaacaagaag tgactggtta aacaatcaga
     1981 cgatcgttct caatttaagt gaaagcttca gtgatcctgc acgtgtccct aatctgacag
     2041 tacctacaga ccctattcct tacatcaaaa gcattataac agaaggaaca ggaggattga
     2101 tgttatccgt cggttatggt ggcggaacag ctaacatgga atgggaagga ctgacaggct
     2161 tgagtatcag taacctgtct gcttcgttag ttacacctta tacccagcta gtggagcgtc
     2221 agaaaataag tccaaatatt accgatctat tcgatgaaaa aatagccatc catcctttta
     2281 cagctgctct ttatcgcaga aaacaagtgt ttgataaatt tggttttgac aggttctatt
     2341 atttagacag tccggataaa ttaacttata cggataaaat agaacaaagc ccacgcatat
     2401 cggatgattc agcatacaaa gaaaccttga aagctttaca aagcaatacg gaaacgtcac
     2461 aattcatcca attatccact atgcaaaacc atatgcctta tgatgattac tatagtgaat
     2521 tgaactacac tgctgaagga actgctgtca ttcctaatag aagaaaagaa ttacaaactt
     2581 atatgcaagg ccttcactat accgatacag cagtaaaaaa attcatccaa gaaatcgaca
     2641 aaatcgataa accgattacc tttgtttttt atggtgacca tttacccgca atctatagtg
     2701 ggaatgattc gaaaaaatat ggattagaac agcaccagac agattatttc atttataaca
     2761 acaagtatag tcgtgaacaa gcagctacgt taaataaaga agttgttgct cctaataact
     2821 ttggtgctat ggctcttgag catgcgaata tcaaagtaac tccttactat gcgttgttga
     2881 cagaagtatc taatcatcta cctgcattaa caattgatcc tacagaaagc ttgtctaatc
     2941 gttttaatgg aaaacaggtc tttgttacag aggatagtca aattatccaa gaaaaaaatt
     3001 taactgagaa acaaaaagaa attctagaag attatcgtct gatacaatat gatcttgttg
     3061 caggtaacca gtacagtgca acatgggcga cacaaaaaat aaaagactaa caaataaact
     3121 gagcaagggg ttgtgataaa agacatgtca caaccccttg tttatctcat taatttgctt
     3181 gtccataaac gatttgatta aagccatttt tcaattcatt attcgctgtc tcgttgaagt
     3241 aaagataatt atcgggattc ccctgatttt gttgtgtcac caatactttt ggctgatttt
     3301 gctgaaaccc gaatagatag acgtgctttt tcagttatgg ttctgtctct gcatcggaat
     3361 agacggctgt gatttgatag tctgcttgac ctgtcccact ttctgaccat tctgcagaaa
     3421 caggcactcc accaatagcc atcttccact ccccgttcaa aatagcttga gggactttaa
     3481 gaccatataa gtccacactc atttgattcg tataactttt gtattgttgt cccattgttt
     3541 ggccccattt ggtcataaat gcctctaatt gtgcggattt gtctgcattc cacagtgttt
     3601 gttcaacttg ttcgctgctt tctgaagagg aagatgaagc ctctacagaa gaacttgaag
     3661 tagatgtttg agttgtttca gtcgttttgg aagactctga cgaagtagag gtatctgctg
     3721 tttctgtact tgctgcagtt gacttgctct ctgtgctagt tgttgtagta gttgcaactg
     3781 ttgctttcgc tgtttctttt gttgattctt ttttcgattc attcccgttg gtacaaccag
     3841 ctaacaaaaa agctagacca attgttacta aaatcctttt tttcaattaa aaaactcctt
     3901 tcgattactc ccacttcttt attctatcaa ataagcaatg tgattttatt gatttttcgt
     3961 attttctcat caatctttca aatcttctaa aaactgagaa gcacccgctg atgttgtctg
     4021 taatacatca gcgggtgctt cttttatttt aatcttcgtg attttccaat ttacctcacg
     4081 ctttatattt gtatttgaag aaacatagac ttttagtcat catcgtcttc ctcatccaaa
     4141 tcttgtcgga cagtttttcc ttggagtctt tgtacttggt agatgatttc tttattgaaa
     4201 cttaaaagtt caacaagcaa ttgttgcaag ccatattttt cttctttttc agctaatttg
     4261 attgcccgtg tgataaagac attttggaaa tcaaaatctt ttgcaaagtt ttccaacatt
     4321 gttgaggctg gtaaatattt gttttgacca tgttcgtaaa tttttccata acgcatgact
     4381 tcttctaaag tagacggaac gatttcattt tcaatcacta actcatgaga gattttattg
     4441 aaccatctcg cttcttgatc agcatattct gaaaaaaggg tacttaattc ttgcgaagat
     4501 gtacccaaaa catacaattg tgcttggatc aatttcattt cttctaattt aaaatttgct
     4561 aatacatgcc ccaccatggc gccagcagtt ggtttatgat ggtcgatatc tgcttgtttt
     4621 gtttcttgaa caaatttttc ttccactata ttcattgatt tacgcctctt ttacttttgc
     4681 tgattggact tcataaccta atttttccac aactgcagct aattgttcag ggtggacttc
     4741 atctggatca aagtttgctt tgattttgct cgcattaaac aaaactttcg tattgcttac
     4801 cccaggctgt tgagaaaccg catgttcgat tttttgcata cagcttggac aagctagtgt
     4861 atctaattgc ataataattt ttttcatcgt agttcttcct tcctttttct tgatagtctt
     4921 attctagatt acagcttcca taaaaaaatt gattcttatc aagttttacg atttttcttc
     4981 tttgctttcg tgtccaaaat acaataaacg catcgcgtta aagataacga tcaagatact
     5041 agcttcgtga acgaacatcc cactagccat gtaaataaac ccggcaaata agccgttgag
     5101 taaaaataag acgacaccaa tggcaatcac tacattttca cgggtattta aaacagtttt
     5161 cttagccaag cggtaggcat gaaccaacga ttcaaaatta gattgcatca agacaacgtc
     5221 actcgtttcg atagcaacat ccgttcctga tcccatagca atcccgatat cagccgcagc
     5281 caaagaggga gaatcattga ttccatctcc aataaaagca acgcgcaatc cttgttcttg
     5341 gtattttttc acaaattgaa ccttttcgtc tggtaataat tcagcatgga cttcatcaat
     5401 gcctaacata tcagctacat aatcagcagt catctgatta tcacctgtca gcatcactaa
     5461 ctgttttgct cctgcttgtt tcaacttagc taattgttca gcgacttctg gtcgaatcac
     5521 atctgacaca cctatgattt gagtgatttt tttgtcgatt gctacaataa tgaccgagct
     5581 gcctgtttgt tgtaattgat gtaaatcttg tacttgcttt ggtgtgagag aaagtttgtg
     5641 agcggcaatc aatttggtgt tgcccgcaca aactgtatga ccatcaatct cagctgtgat
     5701 tccttgtccc ttgattgtct gattatcttt gactgctaat tgttgaaaat caatgtcttt
     5761 ctcctgagca taatggatca cggcgcgccc cagaggatga tcggataacg tttctaatcg
     5821 agccactaaa gctaagctgt ccccttcatc ctctgtatag ttcttgatag tggatacagc
     5881 agtagttcct ttggtcaatg taccagtttt atcaaagaca aaagtatcga ttttggaaaa
     5941 agtatccatt gcttcaccgc ctttgactaa gatgccccgt ttcgctccat taccgattcc
     6001 tgctacattc gaaacaggcg caccaatgac taaagcgcca ggacaaccaa gaaccaaaac
     6061 agtgattgct aaacgaaaat cacgactaat cagaccaaca accaacgcaa taagtaaaac
     6121 ggttggagta taatactgag aaaatcgatc aatgaatttt tccgcatggg atttggtatc
     6181 ttgtgcttct tctactaatt cgataatttt ggcaaaggtc gtatcatctc cgaccttagt
     6241 tgcttcaatc gtcacgtagc catctgctaa aatcgttcca gagaagactt catctccact
     6301 gattttgacg acttgtcgtg attcaccagt cactgcggct tcatccgcat accctttccc
     6361 gtcaatgact cgtccgtcca caggaatagc tccaccagtt tttactaaga cgatatcgcc
     6421 ttcttccacg tcatcgattt ccacttcttc agtcgttcca tcttcttgga caacactagc
     6481 agtagatggg gccatctcgg tcaaagcctt gattgactcc cgcgttttgg tcaatgtttt
     6541 ttgttccaga tagctaccaa acaaaaacaa gaatgtaacg atagctgact ccttgtattc
     6601 tccgatcata aaggcaccaa gcacagcaat agtaacgagt aaatcaattg agataacttt
     6661 gactttcact gcctgatacg catggatcat aataggtatg accgcaataa tagaagctat
     6721 ggctagagca atgttataga acaagacaaa accagtaaat tttccgataa aaccaaaaat
     6781 aatcaacaca gctgataagg cggtgatatg atttcgatgc tcttggatcc acttttgtaa
     6841 tttcatcttt ctccctcttt tcctttcatt cttacgagac taagtataga tacttatcca
     6901 tcataaaaaa ttgaccacta tcaagtttag tcaaaaaggc ggttttcttt tggtagaata
     6961 cactacaaaa aatagttagc aagaagactc actaaaagtg atcttgccaa ctattgtcgt
     7021 ttctatcgaa attttcattg aatgattttc ctgtacagca tacatcggag atctatttct
     7081 ttatctaaga tttttatctt gctaattttt tcctttagtc tttcaaatcc acgtcgttca
     7141 tagaattgat acgtacagcc actatctgta aaaaggaata tttcttttcc tttctctctt
     7201 ttttcaaatt ctgttagcaa cttggtaccg atgccttttg ttttcaaatc aggatttgca
     7261 gctaaaaaaa tgatctctcc atctggcgaa tgcttcttta aatatgctgc aagcatttgt
     7321 tcatttgctg tctcgtaagc tccttta
//
LOCUS       DASOUD010000109         7283 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.110, whole genome shotgun sequence.
ACCESSION   DASOUD010000109 DASOUD010000000
VERSION     DASOUD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.110"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..3319)
                     /locus_tag="U4A29_002285"
     CDS             complement(170..3319)
                     /locus_tag="U4A29_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEO0681392.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="U4A29_002286"
     CDS             complement(3333..4550)
                     /locus_tag="U4A29_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO0681393.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="U4A29_002287"
     CDS             complement(4540..6135)
                     /locus_tag="U4A29_002287"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEO0681394.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="U4A29_002288"
     CDS             6260..7045
                     /locus_tag="U4A29_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEO0681395.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         2132 a         1477 c         1199 g         2475 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt acctcagatc
     7141 cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag tataaattga
     7201 ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta ggagacgatg
     7261 agtttgaaca attagtttaa aag
//
LOCUS       DASOUD010000110         7208 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.111, whole genome shotgun sequence.
ACCESSION   DASOUD010000110 DASOUD010000000
VERSION     DASOUD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.111"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..746
                     /locus_tag="U4A29_002289"
     CDS             99..746
                     /locus_tag="U4A29_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO0681396.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="U4A29_002290"
     CDS             1139..3784
                     /locus_tag="U4A29_002290"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO0681397.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="U4A29_002291"
     CDS             4094..5407
                     /locus_tag="U4A29_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO0681398.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="U4A29_002292"
     CDS             5397..6050
                     /locus_tag="U4A29_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO0681399.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="U4A29_002293"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="U4A29_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO0681400.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="U4A29_002294"
     CDS             6947..7147
                     /locus_tag="U4A29_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO0681401.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2381 a         1219 c         1585 g         2023 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaa
//
LOCUS       DASOUD010000111         7053 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.112, whole genome shotgun sequence.
ACCESSION   DASOUD010000111 DASOUD010000000
VERSION     DASOUD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.112"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..789
                     /locus_tag="U4A29_002295"
     CDS             <1..789
                     /locus_tag="U4A29_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEO0681402.1"
                     /translation="NIYQLVTKKEKKLFLNTTFENLTMINPNIMNFEDESIIEASGFR
                     IQPEYELFKRLFDIIVSLILLILASPFMLITAILVKTTSPGPIIYKQVRITKNQKEFS
                     IYKFRSMSATAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMSIVGP
                     RPERPFFVDQFNEENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSL
                     LLDLKILLQTIKILFDKMSSQGLDEDENISSLAEIDFSQEKIYR"
     gene            848..1651
                     /locus_tag="U4A29_002296"
     CDS             848..1651
                     /locus_tag="U4A29_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO0681403.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            1684..2538
                     /locus_tag="U4A29_002297"
     CDS             1684..2538
                     /locus_tag="U4A29_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO0681404.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            2535..3236
                     /locus_tag="U4A29_002298"
     CDS             2535..3236
                     /locus_tag="U4A29_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO0681405.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            3244..4311
                     /locus_tag="U4A29_002299"
     CDS             3244..4311
                     /locus_tag="U4A29_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO0681406.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            4304..5269
                     /locus_tag="U4A29_002300"
     CDS             4304..5269
                     /locus_tag="U4A29_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO0681407.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            5314..6288
                     /locus_tag="U4A29_002301"
     CDS             5314..6288
                     /locus_tag="U4A29_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO0681408.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            6359..>7053
                     /locus_tag="U4A29_002302"
     CDS             6359..>7053
                     /locus_tag="U4A29_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO0681409.1"
                     /translation="MFIYILRIVIQFVGRSFFIYFLGAKYLGLNGLFTNILSFLSLAE
                     LGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKN
                     STDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIA
                     LYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIG
                     NMSSKIGGVIVMGT"
BASE COUNT         2584 a          942 c         1312 g         2215 t
ORIGIN      
        1 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
       61 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      121 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
      181 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
      241 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
      301 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
      361 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
      421 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
      481 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
      541 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
      601 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta
      661 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga
      721 ctagatgaag atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt
      781 tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa
      841 tgtaagtatg ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt
      901 aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc
      961 tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt
     1021 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga
     1081 acatacgaaa aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg
     1141 attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa
     1201 tattgaggaa tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata
     1261 taacaatgca attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat
     1321 gtttgataaa aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga
     1381 ttattatctt tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac
     1441 attagtctat gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct
     1501 tggttctggt cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga
     1561 ttttataatt gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg
     1621 aaaattttat caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca
     1681 aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt
     1741 ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa
     1801 caagggttta ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa
     1861 aaattttatc aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct
     1921 tctaaaaatt ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt
     1981 attaaaactt atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg
     2041 ttagatgttg cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta
     2101 tatgctttat tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt
     2161 agtaagatac tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt
     2221 gctgaaaaac aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt
     2281 gtcggaccta aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg
     2341 gtgccattcg aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa
     2401 gtttttggtg actatatgca gcttccgcct gaaaaagatc aggtttctca tcatgaagca
     2461 gtttatattg atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa
     2521 ggagcagaaa aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg
     2581 gacaagatat cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt
     2641 tagaatcatt tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt
     2701 gggaagaaac gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca
     2761 ttacaggtgg atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact
     2821 attctgaaga tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac
     2881 tggtattgtc agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac
     2941 cttacaacga acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc
     3001 gtaacacatt gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag
     3061 catatgaccg ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca
     3121 tgatggtaga tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat
     3181 tgacaacaac agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga
     3241 aagatgatga cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat
     3301 catacaccaa aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga
     3361 atgatcggct cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa
     3421 atcaaagttt atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat
     3481 cttgaattag atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa
     3541 attcaagctg actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact
     3601 gatccaatcg gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca
     3661 gtagcaaccc aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat
     3721 cgaggcgata tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg
     3781 agagcaggct accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca
     3841 aaacacgacc tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt
     3901 ttatctgata cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt
     3961 gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct
     4021 gctattcttc atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa
     4081 aactttgatt tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa
     4141 gtaatttttg aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca
     4201 gtacttgaaa ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa
     4261 tcattggttc atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat
     4321 tattgttcct gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc
     4381 ccaaacattt actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg
     4441 gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc ataaagaaaa
     4501 cggcggatta agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg
     4561 ctttgttgac agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt
     4621 gaaaattcat gatgccgaca ttagctctgt tgaattgatt cctttttatg gtgatagata
     4681 taagaaagct aataaagaaa agaaagtaat tatattaaat aaaaaggaag caataaaatc
     4741 tgttttagaa ggaacgcaat tttatgcata cgcatggaat aaactttatc gtaaggaact
     4801 ttttaaagat aatcgttact tggatggaaa aacatttgag gatgcatata taataataga
     4861 tctattattt caaacagaaa aaattgttgt ttccaatgaa gagaaatatt tctatcttca
     4921 aagaaatgat agtattatgg gaaaaagttt ttctatgaat aatttcgatg ttatagaagc
     4981 atggcaatat aacaaagaga agattttaga tgcttttcca gatttgcatg attcctatta
     5041 tcgccgacta tgttgggcat atttttacgt gttggataaa atggttttat caagtagtta
     5101 tcaacaaatt ccacgtaaaa aaacagtaat tgaattttta aagaagaaca gaaattttat
     5161 attaacttat aagggattca ctaaatcgcg caaaatagct attattgctc tgagctttag
     5221 agtagattgg tataggaaat tggttgaaca aaaaaataaa aaaaattaga atgtttaaat
     5281 tagtaaataa aagaaaagga attgaaaact aaaatgtgca gaataagtat aatagttcca
     5341 gtttataatg ttgaaaaata tttaaaaaag tgtgtcgatt caattctagg ccaaacattt
     5401 accgattttg aattattatt aattgatgat ggttctacag ataattcagg aagtatatgc
     5461 gatgaacttg cgaaaacaga caaccgaata aaagtgatac ataaggaaaa tgggggtcta
     5521 agtgatgcca gaaatattgg tattgaggta gcaaagggag attttattgg gtttatagat
     5581 agtgatgatt acattgatga agatatgtat gcatttttat ataacaatat attgaaatat
     5641 gatgcagagt tatcaatgtg tggtatttat gacgtccata agaataaaga aattaaaaag
     5701 ttaactcctt tttctcaatt ggttactaaa tctgaagcaa ttgaattagt attagatggt
     5761 aagctagttg ttgctaatgc agtaagcaag ctatataaaa aagaattatt tgagaatgtg
     5821 agatatccaa aaggaaaaat tgctgaagac gcagcagtaa tactgaaaat tatcaatcag
     5881 tgtaataaaa tccatgttga tacttctcaa aaatattatt attatcatcg tggagatagt
     5941 attactagta aaaaatttac aaaaaaagat tttgatataa ttgatgtatg gaatgagaat
     6001 gaaaaatgga ttattaaaaa ttatcctcaa atgtacgata aagtacatac tagagtttgt
     6061 tgggcttact ttgttgtgtt agataaatta gttttaactg aagaaaaagg actgtatcaa
     6121 gaaaaaaaac aagtaagaga gtatttaatt gataatttct tttttattat aaaaaataaa
     6181 cgattaactt ttcaaagaaa aatatctatg ttattgctta cttttggaat tgctgcgtat
     6241 aaaataccgg ttaaatatca gtataagaaa gtaaaggctg ttaattaatg ctttagttag
     6301 gatgaattag atgaaaaaaa gcaggttaga gtattcttta ataaattctt ctatatcaat
     6361 gtttatatat attttaagga tagtaataca atttgtaggt agaagttttt ttatttattt
     6421 cttaggagcg aaatatttag gattaaatgg tttatttaca aatatactta gtttcttgtc
     6481 gttggctgaa ctaggcatag gctcttctat tatttattcc ctatacaaac cattagccca
     6541 aaaggatgaa agtaaagtaa aggctttaat gaatttgtat aagaagacat atgaatttat
     6601 tggtattttt atcggcatag taggagtatt gattattccg ctgttacctc tatttattaa
     6661 aaatagcaca gacataccac atatgtatcg atattatgta ttatttttat taaattcagt
     6721 tcttagttat tttttcacgt ataaaagatc acttgcaata gcagatcaaa aaaattatct
     6781 ggttgcgatt aatgattttg cctttttatt ctttatgaac attgtacaag taattgcttt
     6841 gtacttttat tctaatttta ctctatttct aatagtgcag attttgttta ctctattttc
     6901 taatataaat atttccagaa cagttgacaa gagatattca tatttaaaag agaaagatgt
     6961 agtttctcta gataagcaaa caaaagatga agtaaagaaa aatgtcatag gtaatatgtc
     7021 ctcaaaaata ggcggggtta ttgtaatggg gac
//
LOCUS       DASOUD010000112         6860 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.113, whole genome shotgun sequence.
ACCESSION   DASOUD010000112 DASOUD010000000
VERSION     DASOUD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.113"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            104..1396
                     /locus_tag="U4A29_002303"
     CDS             104..1396
                     /locus_tag="U4A29_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEO0681410.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            1487..1711
                     /locus_tag="U4A29_002304"
     CDS             1487..1711
                     /locus_tag="U4A29_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681411.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            1828..2325
                     /locus_tag="U4A29_002305"
     CDS             1828..2325
                     /locus_tag="U4A29_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO0681412.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            2412..2804
                     /locus_tag="U4A29_002306"
     CDS             2412..2804
                     /locus_tag="U4A29_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO0681413.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            2788..5235
                     /locus_tag="U4A29_002307"
     CDS             2788..5235
                     /locus_tag="U4A29_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO0681414.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            5238..>6860
                     /locus_tag="U4A29_002308"
     CDS             5238..>6860
                     /locus_tag="U4A29_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO0681415.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLK"
BASE COUNT         2085 a         1339 c         1423 g         2013 t
ORIGIN      
        1 tggatgacac cagcatcttt tttattttcc aaaacatata gaaaagacac cttgaaatcc
       61 tttagaatta atttggcgaa ccaacccaaa aggaggaatc aagatgtctt acactcacct
      121 tatcaaagaa acattagata ttctagactt atcagttact tttaacgaaa attgtctcac
      181 aaaagaaaaa tacaaagggc aaatctgtca tatttatcgt ggaaacttga tttatacagc
      241 ccaagaatgc atccactgta aacaccagat tgcttcagat attgtgcgtt ggggtacaac
      301 aaccgtccgg ttgttaatga acgatgtttc tgaatgccgt acctacctcg aattgaaaaa
      361 gcaacgcttc aaatgcaaag cttgtcagcg gacctttgtc gcagatactt ctgttgcgaa
      421 aaaacactgt ttcatcagtg agaaagtccg ctggtctgtc gtgactcgtc tgaaaaaaaa
      481 tacctcaatg acagagatcg ccgctcagaa aaatctatcg gtctcttctg tttaccgtat
      541 tatgaagcga ttttatcgtc cgttgaatcc ttttcggaca ccgttaccta aggtactttg
      601 ttttgatgaa ttcaaatcag ttcggggtgt ttcaggtgct atgagtttca tcatgatgga
      661 tggacaaaca cagcgcttgt tggacattgt cgagaaccga caactgcctt ttttaaaacg
      721 ttattttagt catttttcac gagaaattcg tgaagcggtc gaatggatcg tgattgatat
      781 gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca aaagcacagc tgatcattga
      841 ccggtttcac atcgtccaac atattggtcg tacctttcgc aatcatcgaa tcaaagaaac
      901 gaaccaacta ttaaaaagca aagaacaaaa acactatcaa ttaggtaagc aattaaaaag
      961 atactggaaa cttcttcaaa aagatgaacg caagcttgat tatactcgac gcctttggcg
     1021 acctggcttc aaagctcatt taacagagac cgatattgtg gatcgcttac tgaaagggag
     1081 ccctgctttg cgtgtaggct atcagttata tcaagacttt ctttacgctg taaaggaacg
     1141 agactacgta tcgtttgaag aactacttac caataatatc atgcttcccg aaggctatca
     1201 gacgaccttg agaacgttcc agaaatttct gccacaaatc aaaaatgctt tacaacaaag
     1261 ttattcaaat ggcccacttg aatgtctgaa caaccatatc aaagtgttaa aacgaaatgc
     1321 atatggcttt cgtagctttt ataatttcaa attgcgcatc atgattcgtc atggcaacgc
     1381 attgattttc aactaaaaaa gatcaagagg aactgatcca cttgatctcc aattattcta
     1441 agatttctct ttaccaacat catttgacaa agagccgata tttataatat caggaactta
     1501 cctatccata tataactggt ttctatcaaa cgctcaatca ctggtgcttt tagcaatcgt
     1561 tgtgattggc ttgtatcttg gcttcaagcg tgagtttagc aaactgattg gctttttaat
     1621 tattgcgatt attgcggttg gcttagtctt caacgctgct ggagtaaaag acattttact
     1681 agagctattc aatcgcatta ttggtgctta aataaaaccg ttcttttgtg gaatataagt
     1741 ggttttctta tgttccgcaa aggaatggta caccaaacga agtgcggtag ggatttttga
     1801 atctctacaa agaaaggacg tgaatatatg gacgatatgc aagtctatat tgcgaattta
     1861 ggcaaataca atgagggcga attggtcggt gcgtggttta cctttcccat tgactttgag
     1921 gaagtcaaag agaaaatcgg cttgaatgat gaatatgagg aatacgccat tcatgattac
     1981 gagttaccct ttacggttga cgaatacact tctattagcg aactcaatcg cctatgggaa
     2041 atggtatcgg agttgccaga agaactacaa tcggagctat ctgctctgct cactcatttt
     2101 tcaagtattg aagaactaag cgaacatcaa gaagatatta tcattcattc cgattgtgat
     2161 gatatgtctg acgtggcacg ctactacatt gaagaaacgg gtgctttagg cgaagtgcca
     2221 gccagtcttc aaaactatat tgattatgaa tcctatggtc gggatttaga cctttcggga
     2281 acgtttatct caaccaatca cgggattttt gaaatcgtct attaaatctg tcggtacatt
     2341 cttactggca gattttcttg tttacggggt agtcaaaaga ctatccccat ttttatgaaa
     2401 ggattgatta catgaagaaa atacgaagct ataccagtat ctggtctgtt gagaaagtgt
     2461 tgtactccat caatgacttt agacttccgt ttcccataac ctttacgcaa atgacatggt
     2521 ttgtcgtgtc actctttgca gttatgatac ttggcaactt gccccctctt tctatgatag
     2581 agggagcatt tctcaaatac tttgggattc ctgtggcttt cacatggttt atgtctacaa
     2641 aaacctttga tggtaaaaag ccttatggat ttttgaagtc tgtcattgct tatgcactgc
     2701 gacgaaagct gacctatgca ggaaaaaaag taacgcttgg cagaaaccag ccacaagaag
     2761 ccattacagc agttaggagt gaattttatg gcatatccaa ttaaatatat tgaaaacaat
     2821 ctggtctgga ataaagatgg ggaatgttac gcttactatg agcttgttcc ctacaattac
     2881 tcatttctaa gtccggaaca gaaaatacaa gtacatgatt ctttcagaca gcttatcgca
     2941 caaaatcgtg atggcaagat tcatgcttta caaatcagta cagaatccag catactttct
     3001 gcacaagaac gttccaaaaa tgaagtcact ggaaagctca aaacggttgc ctatgacaaa
     3061 atcgaccaac agacagacgc tttaatatcc atgattggcg aaaatcaagt ggactaccgt
     3121 ttctttatcg gttttaagtt gcttctcaac gatcaggagt tttctatgaa aagtcttacc
     3181 gttgaagcaa aaaatgcttt tactgatttt gtctatgatg tgaaccataa gctgatgggc
     3241 gattttgtta gtatgagtaa tgatgaaatc ctgcgttttc agaagatgga aaagctttta
     3301 gaaaataaaa tctcccgtcg tttcaaaatc cgaagattag ataaggatga cttcggctat
     3361 ctgattgaac acctttacgg acagacaggc actgcctatg aagagtatga gtatcctcta
     3421 tcaaagaaaa agctggaaca tgaaacactg attaaaacct atgacctcat taagcctact
     3481 cgctgtctgg tggaagaaaa acagcgatat ttgaaaatcc agcaggaaga cgaaaccgcc
     3541 tatgtagctt actttaccat caacagtatt gtcggcgaac tggacttccc gtcctctgaa
     3601 atcttctact accagcaaca gcaatttacc tttccgattg atacgtcaat gaatgtggaa
     3661 attgtagcga atcgtaaagc cctatctact gtccgcaata aaaagaaaga actgaaagac
     3721 ttggataacc acgcttggca aagtgataat gaaaccagct ctaatgtggc ggaagctctg
     3781 gaaagtgtga acgagctgga aaccaattta gaccaaagca aggaatctat gtataagctg
     3841 tcctatgttg taagggtatc agcaaatgac cttgacgaac ttaaacgtcg ttgtaatgaa
     3901 gtgaaagatt tctatgatga tttgagcgtg aaactggtac gaccttttgg ggatatgctg
     3961 ggcttacatg aagaattttt acctgccagc aaaagatata tgaatgacta tattcaatac
     4021 gtgacctctg atttcctcgc tggtttaggt tttggtgcta ctcaaatgct gggtgaaaat
     4081 gaggggattt atgttggcta cagcttagat actggacgca atgtctatct gaaacctgct
     4141 cttgccagtc aaggggttaa gggttcagta accaatgcgt tagcgtctgc ctttgtcggt
     4201 tcgctgggtg gtggtaaatc ctttgcgaat aaccttatcg tctattatgc agtgctttat
     4261 ggggcacaag cagtgattgt agacccaaaa gcagaacgtg gcagatggaa agaaaccttg
     4321 ccagagattt cccatgaaat caatatcgtc aatctgactt ctgatgagaa aaacaaaggc
     4381 ttacttgacc cttatgtaat tatgaaaaat accaaagatt ctgaatcact ggctattgat
     4441 attttgacat tccttacggg gatttcctct cgtgatgggg aacgcttccc aatccttaga
     4501 aaagccattc gtgcagtaac caatagtgaa gtgcgagggt tgatgaaagt gattgaagaa
     4561 ttacgggttg agaatacgcc actaagtacc agcatagccg accatatcga aagttttaca
     4621 gactatgact ttgcccatct gctttttagt gatggttatg tagagcagtc tatcagcctt
     4681 gaaaaacaac tgaacattat acaggttgcc gacttggtac ttcctgacaa ggaaacttcc
     4741 tttgaggaat ataccacaat ggagttacta tcggtagcaa tgctgattgt cattagtacc
     4801 tttgcgttgg actttatcca tacagaccga agtattttca agattgtaga cttagacgaa
     4861 gcatggagct tcttacaggt ggcacaaggg aaaacactat ctatgaagct ggttcgtgct
     4921 ggtcgtgcta tgaacgctgg ggtatatttc gtgacccaaa atacagatga cctcttagat
     4981 gaaaaactga aaaataacct cggcttaaaa tttgcctttc gttccactga ccttaacgag
     5041 attaaaaaaa cccttgcctt ttttggtgta gacccagagg acgaaaacaa tcagaagcga
     5101 ttgcgtgatt tggaaaacgg gcaatgcctt atcagtgatt tatatggtcg tgtcggtgtg
     5161 atacagttcc accctgtatt tgaagaactg ctccatgcct ttgataccag accacctgtg
     5221 cgaaaagagg tgtaaatgtg aaaccatcaa tattaaacag aataaaatca aactggacgc
     5281 tgaaacgtct aggtaaagtg gcaatgacag tggctttcac acttgtgatt gccatttttc
     5341 ttttagccat gctgggaacg gtggttcaag ctgcgggctt ggtagatgat acggtcaatg
     5401 tggcaaatga atacagccga tacccacttg aaaactatca actggatttt tatgtggata
     5461 atagctgggg ctggctaccg tggaactggt cggacggaat tggaaaacaa gtcatgtatg
     5521 gactatatgc cattaccaat tttatttgga caatcagtct ttatgtttcc aatgcgacgg
     5581 gttacttagt acaggaagcc tattccttag acttcatttc cgctacagca gattccattg
     5641 gcaagaatat gcagaccctt gcaggggtta cgcctagtgg actatcaaca gagggtttct
     5701 atgttggatt ccttttactc ttgattttgg ttcttggggt ttatgttgcc tatacaggac
     5761 tgataaagag agaaaccaca aaggcaattc atgccctcat gaattttgtg ctggttttta
     5821 tcctatcggc ttcctttatt gcctacgctc ccgactacat taaaaaaatc aatgactttt
     5881 catcagacat cagcaacgct agtttgtcac tgggtacaaa aattgtcatg ccccattcag
     5941 atagtcaagg caaggacagt gtggacttga tacgtgacag cctattttcc atacaggttc
     6001 agcaaccgtg gctactgctt caatataaca gttcagacat tgaaagtatt ggtattgacc
     6061 gtgtggaaag cctgctctca accagtccag attccaacaa tggggaagac agagaaaaaa
     6121 ttgttgcgga agaaattgaa gatagaagca ataccaatat gacgattaca aagacgatta
     6181 accgtttagg tacagtcttc ttcctatttg tcttcaatat tgggatttcc atatttgtat
     6241 tcctattaac aggaatcatg attttctcgc aggtactttt tatcatctat gctatgtttc
     6301 tgcctgtgag ctttatttta agcatgattc catcatttga tggtatgtca aaacgagcca
     6361 taacaaagct ctttaatacc attttgacac gagctggaat cacattgatt attacgacag
     6421 catttagtat ttcaaccatg ctctatacct tatcggctgg ttatccgttc tttttgattg
     6481 cttttctaca gattgtgacc tttgcaggaa tctactttaa gctgggcgat ttaatgagta
     6541 tgttttctct acagagtaac gattctcaaa gtgtgggaag tcgtatgatg agaaaacctc
     6601 gtatgcttat gcacgctcac atgcaccgtc tacagcggaa acttggacgc tccgtgactg
     6661 ctttaggggc tgggtctgcc attgccagtg ctacgggtaa aaaaggacag tcgggttcgg
     6721 gaagttctgc gagtacacaa gcagatcact ctcgaccaaa cgggcaggaa aaatcaacac
     6781 taggaaagcg tatcggtcaa accatcggta cagtagctga taccaaagac agaatggtag
     6841 atactgctgg taatttgaaa
//
LOCUS       DASOUD010000113         6826 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.114, whole genome shotgun sequence.
ACCESSION   DASOUD010000113 DASOUD010000000
VERSION     DASOUD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.114"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..447)
                     /locus_tag="U4A29_002309"
     CDS             complement(<1..447)
                     /locus_tag="U4A29_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0681416.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(754..1851)
                     /locus_tag="U4A29_002310"
     CDS             complement(754..1851)
                     /locus_tag="U4A29_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEO0681417.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1959..3986)
                     /locus_tag="U4A29_002311"
     CDS             complement(1959..3986)
                     /locus_tag="U4A29_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEO0681418.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4131..4862)
                     /locus_tag="U4A29_002312"
     CDS             complement(4131..4862)
                     /locus_tag="U4A29_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEO0681419.1"
                     /translation="MMKKEIVLTAAIMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            complement(5378..6277)
                     /locus_tag="U4A29_002313"
     CDS             complement(5378..6277)
                     /locus_tag="U4A29_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEO0681420.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(6287..6643)
                     /locus_tag="U4A29_002314"
     CDS             complement(6287..6643)
                     /locus_tag="U4A29_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEO0681421.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
BASE COUNT         1942 a         1385 c         1102 g         2397 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat attgttataa aaagagccca accatcctac
      721 aataataaaa atttactatt tgttatctta accttacttt tctttctctt ctttttttct
      781 caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag ctactaagat
      841 ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt ccttggctac
      901 ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt tgatcgtagt
      961 atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa cagtcattgt
     1021 aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa aagaaaaatt
     1081 tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt cagaatgtcc
     1141 tttttttgta atttttgttt caataactac ttgattgata taggtttttt gatcattttg
     1201 aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt catgaagagt
     1261 aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa tataggtgta
     1321 ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt ccgtctggat
     1381 ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg gcatcctgac
     1441 atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg gatagctcac
     1501 ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt ttccgtattc
     1561 aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt tcacttcttt
     1621 ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat caagttgaag
     1681 atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg tcactgaaaa
     1741 attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa taactaggag
     1801 tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca tatgtaagag
     1861 aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc tgtgcccttc
     1921 ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg gacatctgca
     1981 agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac aggaatatct
     2041 attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct ttcacccttt
     2101 tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt ttcagaggta
     2161 acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt ctcttgtttc
     2221 aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc acctgaaaac
     2281 tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt gacatattga
     2341 ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc ctctgtattt
     2401 tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc ttcaatatca
     2461 ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat ttcagcaata
     2521 tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt cagctttgcg
     2581 atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag gtattcaaaa
     2641 tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa ttcgattatt
     2701 aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt tccggttatc
     2761 aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg aatagttaat
     2821 tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc cccctcgacc
     2881 ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat ggccccataa
     2941 tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca
     3001 gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca atttacttct
     3061 gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagcgacc gacaccttcc
     3121 aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg
     3181 ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga ataattatta
     3241 ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc agcagttatc
     3301 tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc aatcgagagc
     3361 gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc catacctggc
     3421 gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa tgtaaaggtc
     3481 atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt aaatgtaaaa
     3541 tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga cctttctgct
     3601 gggttcaact gcagtaactt cccattttcc aatatggacg tgctagtacc gataggtgtt
     3661 tgaaaaattt tatcgataga aataatattg ggatcgtcag gcagctcaac cgcagaaatt
     3721 ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt ctacgttgtc agtggtctct
     3781 ccactactcg aaatacttaa atcctcactg ctttcttgtg tctggttgga ttctattaaa
     3841 ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc tatttccatg ctccaactga
     3901 ttcaattcca aagagtaccc tatgatcgga aagatattag tcactaagag actgcttaaa
     3961 atgagcacga tcctcctctt tttcaatata tcttttcctc ctgatcgtta aattcctaga
     4021 tgaactattc gtatccaaac aaatttagcc tagcctttga caatttttgc tcgattacct
     4081 cgcaagacaa tatgactacg tatttatttt taacagttct tttcagtaaa ctagttgtta
     4141 ggtgtatctt ctaatgtcca aactaattta gtggaatatt gttgcgctaa cttgacagat
     4201 ttaccgggta ccgaaagctg taccgcatct tgattttcat tttcatttgc accaaatcga
     4261 tagacccaag tccccacacc tgttcctgat ttagcattaa ttgctaacgt attgtttcct
     4321 cccggtgtca acgttaattc agaattcacc gtttgcggcg tatatgttgg atcaaccaaa
     4381 gaaacagcct gccctttcgt aaatttcagc tgtgctccaa ctagttcatc cccactggca
     4441 gttttaaact gttctggttg agagacactt aatttccacc cagataaggt acttctccta
     4501 tctgtcactt ggacatatgt cggttttcta gacccatcgt tcatcacatc agcagctgca
     4561 taatacgttt tatccgcagt agaaattttt tgagtcccaa atttgaattt tgatccataa
     4621 tcaatcgata atgcccctcc tgttccaggg cttggaggat ctgatggatc gacaggttta
     4681 ttgggatcag gattctctgg atcaacagga ggagttacac cctcatctcc tgcttcaaat
     4741 gtaatcgttc cttctgtgtt atattccttt gggtatactt cagttgcaga aacaaaagtc
     4801 gtacttgcta ccatgctgaa taacattatt gctgctgtta atacgatttc ttttttcatc
     4861 attgacattc ctctttcttt atttactaaa catatcagtc gattggatta atccatagca
     4921 aaattctgtt ttacgatact tatgaaataa aagaacatag acctatattt ggtcctatgt
     4981 atcgattagt tataaatgat tatcagcccg atttgatttc tagtttttat tgcaacttga
     5041 tgcaaacaca atctagaaat acagctgtca tagcctttaa aataattcgt aagtccccct
     5101 cctatctatc aaaatgtgac ttgactgcct agacttcaat caatattaat tataattaaa
     5161 taaaaaatgt atattatatt ttttttgtga ttatcgttca aaaaaagaat acacgcttgc
     5221 atctagtact ctgcgagtta caaatcatcc gatcaaataa acattcgctg attattttcc
     5281 gcaaaaaaag cagcatatcc acgataaatg gatctactgc tcttacttat ccttatgttt
     5341 tacaaggtat tttcttttac acattactaa tgctgtctca atacggaacg attgtcatcg
     5401 cttgttcaaa ttcctcttgc aaatgttgtg tttgataggc ctgaaaagct gggtgatggg
     5461 cacgcaagag gacacgtaat ttcataggat catagctgtc tagcaactgt tctttcaaga
     5521 caaaatctcg ggtcagctgc cattcataat aataaccaaa aggtctgcag ctgtaaatcg
     5581 tgtgaccgat ggatttatct tcaaagcgac gatgcgtatg gccaaaaact acttgtttga
     5641 tatttgaata acggtcaagt aattctccga atgattctga tcctaaaaat gcatttaatt
     5701 tgttccaacg ctcataagga gcagattgat acatgataaa tgcccgttta ggaacgaaat
     5761 gagtggataa aatcacctca agtttttttg cttccagctg gtctagcaaa ttttttgcag
     5821 ctgcaagcat ttctgcattg acctcgagat ctgttttccc acgctggatg aaccgatcat
     5881 accaatacaa acgcttcatt cgtacgattg cctcgtaatc tttcaattcg gaaaactgat
     5941 agtcgtacca gccattcatc cctaaaagaa cggtctgctc atttaaagca atataccgtt
     6001 catttaaaaa atgtgggtct ggaaaatggt ccatcatctg ttcattgggc acatctgcca
     6061 actcgtgatt gccgaaatga aatgtcgtag ataatccttt attgacgaaa aattcgacaa
     6121 cagctaggca acggtctact ttattagccg tatctccggc tagatgaagc cgagtgatct
     6181 tgtgcttttg caaaacttgc caaagctgca tcagctctga ttcgccgaat ttattgatgt
     6241 ccacatgtag gtcgctgatg atcgccaatt ttcccatttt atccctctag tcttttcgaa
     6301 caacttgcaa gttgcctttt ctcaagccgc ttggtccttt taattgtaaa ctattataag
     6361 ttggttctaa agcaagtgtg gatggatcat ccatgtaaag tattgcactg tctttgattt
     6421 tgattggtcc aactggtgta tcagccacta cagaataaag acttagatct gtttgtgtat
     6481 cttttttgac aatgatcaat cgaaatgccg tatccaattg gcaactgact tggctttctg
     6541 caaatggacc gtcaccgttt tcgatatcta ataaaagttg atcgtctgct tctaatttat
     6601 tcttcaatac ttcttgtgct tcttttgtca cttctaattt catctgtatc acctcatgtc
     6661 atagaataat cactttattc atcagatgca actaatttgt ctggttctga ctgttctcaa
     6721 tttattggaa agtaagtcat tcgtttatta tgatttctct tatcacaaaa gagacaactt
     6781 tcccttttct tcgttcagtt tcttgaacgg aaaaagaccc aaaaag
//
LOCUS       DASOUD010000114         6763 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.115, whole genome shotgun sequence.
ACCESSION   DASOUD010000114 DASOUD010000000
VERSION     DASOUD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.115"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(41..1420)
                     /locus_tag="U4A29_002315"
     CDS             complement(41..1420)
                     /locus_tag="U4A29_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEO0681422.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1441..2988)
                     /locus_tag="U4A29_002316"
     CDS             complement(1441..2988)
                     /locus_tag="U4A29_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO0681423.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3237..4016)
                     /locus_tag="U4A29_002317"
     CDS             complement(3237..4016)
                     /locus_tag="U4A29_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO0681424.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4091..4549)
                     /locus_tag="U4A29_002318"
     CDS             complement(4091..4549)
                     /locus_tag="U4A29_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO0681425.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4561..5835)
                     /locus_tag="U4A29_002319"
     CDS             complement(4561..5835)
                     /locus_tag="U4A29_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEO0681426.1"
                     /translation="MEDLIMKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREAR
                     NYLINQMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVV
                     TALETMRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVK
                     NRHGQTKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIP
                     GIGRFKARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIK
                     PNSNQFVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARIN
                     YSNPTNPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKG
                     GITHHPDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5936..6250)
                     /locus_tag="U4A29_002320"
     CDS             complement(5936..6250)
                     /locus_tag="U4A29_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO0681427.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6396..>6763)
                     /locus_tag="U4A29_002321"
     CDS             complement(6396..>6763)
                     /locus_tag="U4A29_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681428.1"
                     /translation="MQKNPRTLEACIVILGKLFEFIAVEEINLYLDKILRKFVNTPNT
                     DIVEIWLQRLSLIHSREKSYSAKLCKKVSNPKGFTLWNSDWLKKGFDESGIVNETFIS
                     NLSLTTPAKDLELFISQYE"
BASE COUNT         2027 a         1469 c         1079 g         2188 t
ORIGIN      
        1 atcatttctt ccgttttaaa gaagaaatga ttttcataga tcaaccttta actaaagcta
       61 gattcttttc taaaaagaat ggtgtgtagt agcccacacc gctggacaac ggtaaaactt
      121 catccggcaa ctcgatgccc agttcgttac ggatgtactc ttggcgagaa atgattcgcg
      181 accaaacatt tggatattct ttggctagtt gatcacgaag cgactcatca gctagcgcaa
      241 tcccactctc acagcttgcg ccggcatagc cagcaactga tggaataata tcaatttgta
      301 gtaacattcc ggacttaaga gtaatttctg agtctttttt cattggcgaa gacatccact
      361 cttcatcagc cgtaagatgc cccggattta agtgccattt gtattccgat ttaggcaaga
      421 ctttacctat tactgaatat agctctttac cttgcattcc tattttgata ttttccaacc
      481 atgcgaccac ggcgtcgtag tatggagctg caactttttc aatgtaatcc ttttgccctt
      541 ctggtaggtc atctgtcgtt gcaactgcat agccacttcg acttgctaat ccgcctttaa
      601 agcccgtagt aattgacatt ttatcgccta gtttaacttg tttatcagtt ggatatatat
      661 aggcgttctc aaaccgttcc ccagttgcag caattggaat gacattattt ggttggccgt
      721 acttaactaa gtggttacca atttccattt ctgtttttcc tacctctact tcatcaatag
      781 cgttcatcac acaatctgaa gccaaggatg agccaaattc ataatatgcc acttcattag
      841 cagtatttaa tgagcgtact ccgtaatcac tgtgaatgaa caggtcggtg cgattgctaa
      901 cggcatcttc ccctactaat tctacaatcg aacttacaat gaatgccggc aaatcttgga
      961 tttggtagtt atcctcttct gaagtaaaca tcttccaacc aacaagccca actttcttac
     1021 ctttactgat tttagcattg gtcaatactt cagttagtgg tttctcgtta tccatcggtt
     1081 gatccggtaa tgagaagagt ggcgtgtgga ttaattctgc ctcaattctt gaatagtcca
     1141 ccattttagt attttcattt cctagaacta agaaagcttc acccgtctga tgtaatacca
     1201 acaagctttc ttcaaagcga gtaacaaaac ctgttaaata agagaagttc ccgccatgtt
     1261 ccaaatcaca atagattacg agggtatcta tctctgactt ttccatcgct gcaagtacct
     1321 tttctttacg gaacttcatt gtttcatcag tcaattgata tattttagct tcattatctt
     1381 ttttaggtgg gttaactttt tttagagtta ccttagtcat attgtcacta ctttctgttt
     1441 ttatttacgc ttcatttcat ttttcagaga tcgagttgcg ttttccactt gtcctccata
     1501 taccacttgt aaattatttt cgttaatttt aacgattccc attgagccgg tctttttcaa
     1561 gatatgttca tcaattgttg ctgtatcttt aagaactagt ctcagccgag agatacagtt
     1621 atcgatttct tgaatattac cagcaccacc aagtccttgc aagattagtt tgccaagttc
     1681 gttttctgtc atttcttcgt cctgatcttc caacacatca tcgttggatc gaccaggagt
     1741 tttcacatca aatcgctcaa tgacatactt aaatacaaag taatatgcaa ctgcataaac
     1801 aaggcccaat gctacaacta agtaccaacg agtataagta ccttgtaaaa ttccgaagat
     1861 agccaaatca ataaatccac caccggcatt cccaactgcg actccgaata gattcattaa
     1921 cataaaggat aaaccagcca tgaatgcatg gaagacccat agaatcggtg agataaagat
     1981 aaaggcgaat tcaattggct cagtaatccc ggttacaaaa caagtagccg ctgctgcaat
     2041 caacatccct ttaatctttg gacgttgcgc caaatatgaa gttttgtaca ttgcatagca
     2101 tgctgccggc aatccgaaga ccataaatgg atgagtacct tgtgtcaaga agcgagtagc
     2161 acttctcaaa atatccatgt cttttactgg caatgctaat tcagtattga agatggttaa
     2221 tgctccagaa actgtttcac cattgatagt tgccgtaccg ccgattggtg taaatcgaat
     2281 caactggttt agtacatggt gaagacctgt tggaatcagc aggcgctctg agaagccata
     2341 taaaaaagtt ccaattacgc ctgacttacc aatcaaaccg cctaaagcat tgatagctgt
     2401 attgacaaaa ggccagatga aaaatgaagc gattgagatg attggaatag ttaggatgat
     2461 cataattgga acaaaacgat tgccactaaa gaaactgaaa gctgctggta attgttttgt
     2521 gtgatagcga ttgtggatca tggctgtcca taatcctgca atgataccgc cgaaaacacc
     2581 catacgatat gtgaatgtgc caagtaaagt ttcatacata ttggaagttt ctactgcttt
     2641 gatttgactc attccgtcag acatcaatgt ttcaacagaa gtgctttccg ctgtgatgcc
     2701 tctaagctga agcagataat tgataactac atggaacaga ataaatgcaa taaccactga
     2761 aaatgctgca atttccttat cttctctaga catcccgatt gctactgaaa tagcaaatag
     2821 aataggaagt agtccaaaca tcgtaccaac aatagcctgt actccaccaa caaatccttg
     2881 gatcacggtt gcattagcga agctttctcc caccaaagta ggattttgta aaattgaaac
     2941 gattcctagt agtagtcccg atgcgacaat aatagacaac ggacccatta atgatttccc
     3001 taacttctgg atataagttc tcaatataaa caccttcttc tttttagttg atataaacgt
     3061 ttacatccct tgtacaacga aagtatagca ccctgaaaaa gcgctaacaa tacgataggt
     3121 ctgataaaaa tcaacattca gcacttggtt gttcattcaa aacgtgaatc ttcaatcaca
     3181 gaaaaatacg cgctatcaag cacgtatttt aggtgtaaat atatgcatca actttattaa
     3241 tcaacgctac tttgtttgta atagttaata tacccttcat acagtaagct aagagcaaga
     3301 ttcaaggtgc agaatgatga gttgttcaac tcattcacat cattaatgtt ggtcacttga
     3361 tagtataggc tatagtctga cagactggct aagttatttt gaccaaatcg tgtaactgag
     3421 accaactgtg tccctttcat atggattctt cttaaagtat catcaatccg atcaacacgt
     3481 ccggacaatg atatgacaaa tagtaaatca ttcgaggtca aagcttttga aatcaggtct
     3541 aattcattct tttccggtac aacgataatg tagattccca agttccacat gcagcgtgca
     3601 aactcattga gcatcattct ttgtccatat cctgttgcgt aggcaaatat cttatctgcc
     3661 tttctaatcg tttcatatat atcctcaaca cttttatcat cttcaaacaa tttggttgtc
     3721 gcttgaacat ctccgagtaa tacttccggt aaaaacaaac tacttttatc gactctggtt
     3781 ttttgcatgc gtgcaaattc ctctttaagg taatatcgaa attcgccata tcctttgaat
     3841 ccgagtttct tagttagtct aataatcgtt gagtcagata catttgcttg ttttgctaat
     3901 tccgatacag tttgactgat gctgatttcc atattttgag tgataaaact taatacataa
     3961 tcatcattct cattcaattt actgtgatgt tcatttacta attgctccaa tgtcataatc
     4021 agacctccaa aaataataaa aaagattgct ctctcatttt aatacgaaag cacaatcttt
     4081 ttaagtacta ttaaattttg aaaagaagtt ctccttcaga aacctttttt tcagagactt
     4141 ctagtttaat acccttatct tcgggaaata gaaccataac aacatctgac agtttttccg
     4201 ccttgatttg gtcacaatca aaaatcacca atgactcgcc ggatgatact cgttgacctt
     4261 cttcaacgag cacttgaaac ccgactccct ttaactgaac agtatcaatt ccaatatgaa
     4321 tcaataaatc tctaccttga ttactagtca atgtgagtgc atgctttgtt ggaaatatcg
     4381 atttaatgac accgtcaatc ggtgctacca ccttagcgtt tgttggttca acagcgaatc
     4441 cttcgcccat catcccttgc gaaaatacag gatcgcttac ttggctaagg ggaatcagat
     4501 ttcctgatac tggagcaact aatttttctt ttcgactaaa aaatcccatt gacttactcc
     4561 ttaatctttt gaaatgtcca tcacaaggtt taaaagaaca tccgcccctt ttttgacatc
     4621 ctcaaaatca gtccactcat caggatgatg tgtaatgcca cctttggatg gaatataaat
     4681 catatttgtt gggacaaagt cggtcatcat catcgcatca tgtccagtac cattgttaat
     4741 gacttgatag ctatagccca actgatcgca aatatgtttc atcttctcaa cattaccttg
     4801 atccataaca cttggatttg taggattact gtagttaatt cgagccattt cctccagctc
     4861 agttctaact ccagtttccc cttgaatcac tgataactca ttcaacaact tttctttaat
     4921 ctcttgagga ttagttgtct ttgtatcaaa ggttctgatt tcgattaaag cttcgacaaa
     4981 gtcagggaca aattgatttg agttcggttt gattgtaagt ttaccgacgg ttccggcaat
     5041 cccttcacca agattcttca tccactgttc aacaaaaaga acaaatttag aagcagcaac
     5101 taaagcatct ttccgtttat tcattggagc agtcgagtcc tcagtttctc cataaaaacg
     5161 tgctttaaaa cggccgatgc caggaataaa ttcaatcaat ccgatatctt tgccttcatc
     5221 ttccaggact ggtccttgtt cgatatgaag ttcaataaaa tttttgattg tttttggatc
     5281 tcgcttagcg gcctctaaat ctggttccac tccataagca cgaagagctt ccaattttgt
     5341 ttgtccatgg cgatttttta ctgtttccaa ctcactcatg gttaatgttc ccatcatagc
     5401 tcgtgaattt gagacacccg tacttggccc aaatgtctcg ccctcctctg cattcatcag
     5461 aattaggtca attgggtaat cattttcaaa attattttct tccagaatcc gcattgtttc
     5521 cagtgcagta acaacgccag ccactccgtc aaatctgcct ccattaacaa ctgaatcata
     5581 atgagagcca atcatcactg atggtgcatc agccttttta ccggctctac gaccaaataa
     5641 tgtagagaag ccgtcttccc aaatctctaa acccagttgt tccatttgat tgatcaaata
     5701 atttcttgct tcacgatctt ctttagagta agcagagcgg gtcactcctt cacctggagt
     5761 tgcattgtac tcggctaaat tttcgatatc cttttttata cgctcaaaat ttgtcttcat
     5821 aattaaatcc tccatttcct acaagtgaat gttagcaccc atagaaagcg gctacaatat
     5881 aatcaatgaa gattgaatcg atattcgaaa actgaatatt gcttcggtta atgacttaga
     5941 aatcattatc gtagattcca ccataagaac tttttctcgt ctcataacac aactcgagag
     6001 tggattggtt taaaaaactc tcgataaatg cgtccacttt attgacacag atccatggat
     6061 cgatcgcacc ttgaaattta aacccagaaa tagtcccatc atcttctata ccgattacca
     6121 gcttacctcc atcggcattt gcaaacgcta ctatgtgttt tataatatct ctaggcttaa
     6181 tccgtgcact ttttctatca aaatactgat cttctttttc acttataaac ttttgaatct
     6241 cataatccat ttaaaatttc ccttcttaaa tttttttatt atagagccgt atattcaatt
     6301 tccacaaaaa aataatctaa aacaaaacag acaccttcat tttgatatgt tctcccacat
     6361 atgaatcggt cgctatacag cattacatat agtaattatt catactgcga aataaatagc
     6421 tccaaatctt tagctggcgt agttaagctt aaattcgaaa tgaaagtttc attgactatt
     6481 ccactttcat caaatccctt cttcaaccaa tcagagttcc acaacgtaaa tcctttagga
     6541 ttagaaactt tcttacataa ttttgcacta taggatttct ctcttgaatg aattaaagaa
     6601 agtctttgta accaaatttc aactatatcc gtattaggag tattaacaaa ttttcttaag
     6661 attttatcca agtataagtt gatttcttct acagcaataa actcaaaaag cttacctaaa
     6721 atcacaatac aagcctccaa agttctagga ttcttttgca tta
//
LOCUS       DASOUD010000115         6679 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.116, whole genome shotgun sequence.
ACCESSION   DASOUD010000115 DASOUD010000000
VERSION     DASOUD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.116"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            201..680
                     /locus_tag="U4A29_002322"
     CDS             201..680
                     /locus_tag="U4A29_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681429.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="U4A29_002323"
     CDS             713..2941
                     /locus_tag="U4A29_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO0681430.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="U4A29_002324"
     CDS             3070..4713
                     /locus_tag="U4A29_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO0681431.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="U4A29_002325"
     CDS             complement(4786..5331)
                     /locus_tag="U4A29_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO0681432.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="U4A29_002326"
     CDS             complement(5354..5839)
                     /locus_tag="U4A29_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO0681433.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="U4A29_002327"
     CDS             complement(5829..6641)
                     /locus_tag="U4A29_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEO0681434.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
BASE COUNT         2290 a         1260 c         1342 g         1787 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcagcgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaatttt
//
LOCUS       DASOUD010000116         6589 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.117, whole genome shotgun sequence.
ACCESSION   DASOUD010000116 DASOUD010000000
VERSION     DASOUD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.117"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="U4A29_002328"
     CDS             complement(99..1229)
                     /locus_tag="U4A29_002328"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO0681435.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKE
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="U4A29_002329"
     CDS             complement(1878..3080)
                     /locus_tag="U4A29_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO0681436.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="U4A29_002330"
     CDS             complement(3140..3343)
                     /locus_tag="U4A29_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEO0681437.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="U4A29_002331"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="U4A29_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="U4A29_002332"
     CDS             4880..5692
                     /locus_tag="U4A29_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0681438.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1998 a         1329 c         1029 g         2233 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaatttctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggtgcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcataga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct actttttta
//
LOCUS       DASOUD010000117         6429 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.118, whole genome shotgun sequence.
ACCESSION   DASOUD010000117 DASOUD010000000
VERSION     DASOUD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.118"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            235..1560
                     /locus_tag="U4A29_002333"
     CDS             235..1560
                     /locus_tag="U4A29_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEO0681439.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1553..1903
                     /locus_tag="U4A29_002334"
     CDS             1553..1903
                     /locus_tag="U4A29_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681440.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1885..2070
                     /locus_tag="U4A29_002335"
     CDS             1885..2070
                     /locus_tag="U4A29_002335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681441.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(2974..3249)
                     /locus_tag="U4A29_002336"
     CDS             complement(2974..3249)
                     /locus_tag="U4A29_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEO0681442.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3221..4174)
                     /locus_tag="U4A29_002337"
     CDS             complement(3221..4174)
                     /locus_tag="U4A29_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEO0681443.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4786..6279
                     /locus_tag="U4A29_002338"
     CDS             4786..6279
                     /locus_tag="U4A29_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEO0681444.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
BASE COUNT         2224 a         1048 c         1167 g         1990 t
ORIGIN      
        1 atttttttct aatataacta agcaaaactg gataatacga cacttatttt caaattttca
       61 caagactaat agtaaatatc catttaataa agtgatacaa tgtgttgatt ttacaaatca
      121 acagaagaag tagggaaata acttacctat gcttcttttt ttgtacattt aatcgattct
      181 tctttacaaa aacgaacgta tgtttgtata atgtggaaga atggagtgtt atttatgatg
      241 gattattcaa aagagccagt aaatgattac tttttgatcg atatgaaaag cttttacgca
      301 agtgtcgaat gtatagaaag gaatttagat ccattaacga ctgaattagt cgtcatgagt
      361 cgagcggata atacaggatc agggttgatt ttagcttctt caccagctgc aaagaagaag
      421 tacggtattt ctaatgtcag tcgaccacga gatctaccgc aaccttttcc taaaacgtta
      481 catgtcgttc caccacgcat gaatctctat attaagcgaa atatgcaggt aaacaatatt
      541 ttcagaaggt atgtagcaga cgaagattta ttgatttact cgatcgatga atcgatctta
      601 aaagtgacca gatcaatgaa tctttttacg actgaaggaa caagaagtca acggagaaag
      661 aagctggctc aaatgatcca ggagaatatt aaagaagagc taggattgct tgctacagta
      721 ggtgtcggag ataatccctt attagctaaa cttgctttag acaatgaagc aaaacataat
      781 gatggattcc ttgctgaatg gacgtatcaa aatgttcctg ataaagtatg gaatattcct
      841 gaaatgacag acctttgggg tattggatct cgaatgaaaa aaagactcaa ccaaattggt
      901 atcatgagta ttaaagactt agctaattgg aatccataca ctattaagag ccatttaggt
      961 gtgattggct tacagctgta ttttcatgcg aacgggatcg atcgaacaga tatagcgatc
     1021 ccaccagaac cgacaaagga aaaatcatat ggtaattcac aagtgttacc acgagattat
     1081 accaggagaa atgaaatcga actggtggta aaagaaatgg cggaacaagt agctattcgt
     1141 atcaggcaac ataactgtaa aacaggttgt gtccatttat acattggcac gtcgatctta
     1201 gaaacgagac caggtttttc acaccagatg aagatcccta taacagataa tacaaaggaa
     1261 ttgcagaatt actgtctttt cttgtttgat aagtattatg aaggacaaga agtaaggcac
     1321 gttggaataa cctatagcaa gcttgtgtac actgattcgc ttcagctgga tttatttagt
     1381 gatccacaaa agcaaattga cgaagaaaat ttagataaaa ttattgacaa gattcggcaa
     1441 aaatatggtt ttacgtcgat tgtacatgct tcttctatgt tagaaggcgc tcgtagtatt
     1501 actagaagta cattggttgg tggccatgca ggcggcgcag gagggcttaa aaatgattga
     1561 ttataatgat cgtggacgtt taaagtgggc aggtttttat ttatctgatc atactgaaaa
     1621 aattgatgcc gacaatgaac aacgagccaa tcaaaatttg gcaaaagaac aaatgacgac
     1681 tgaagaggtt gcggaagtat tgaacgctgc agtcataaaa aatagatcag tatgtattca
     1741 aaaagaagaa cgaaacgctg aaggatacta tccagaagat attgttagca agatcttagg
     1801 tagtgatgag ctggggatct acgttggaaa aaacaaagtt cactacgacg aaatacgtca
     1861 tatcgaattt taccaggata ataaatgggg tcgattagaa tgaacattat ccagcaatat
     1921 gagctaaagt atataacctt tgatcagcta tcagaagaga tttggggtta tggccagcgt
     1981 ttgataaatg aggttggtgt tgaacgcttc tctttctatg tagaagctgc agcaggatac
     2041 cataaatacg aatactatat tggctattaa agtgcaataa aattatattt cttttatgtt
     2101 ttacattttg tatcaattct gataaaatta cctataggta attaaaaaat ataaaaaaac
     2161 tagttacaac cttaggacgt tgtaactagc ttgaagcata ctagagctaa ctaaaatagc
     2221 actagaaagc aatgttaaac ccgtccaaca aacttgattt aacattgaga ccagcttatt
     2281 cagctaaata tagttgaata agcttctttt gttttaaaaa aacaaaaggt gtactacgaa
     2341 taacgcaagt aaaagaaata ctattacaac cagtaggcta caagtaagta ttactaaaca
     2401 ttgcgtagtt agaacctttg ctctattgtc taaagccaca aaattagaga tcagctttat
     2461 gatgctaaga gcgagctgca tccatgcgga cataggttct tttgacacta ttaattctcc
     2521 tttttaggat aagggaaatt gctagtagtt ttttcattag caccatcccc cttcatttga
     2581 ttgagtctag tgctgctgtg atagtttgct gtttaatatg cttacattct tattataaca
     2641 taaatgaaac aaacacatgg ttagaggtcg tatcaatcaa aatagtgtct cacaaacgat
     2701 ttattactta ctcctgctga ctaacggact gttcttcagt aactgtaact atatgtttgt
     2761 taaaaccacc attacctgta gataaaaacc aatatattta ctatactcac tatattaaat
     2821 atatttagta tagtaaaaac tagaaacaat aaaatttgtg aatgttgtat acccccaaac
     2881 aatacacata ttttgaagga ttaggattaa taaagctaaa aatatagtaa gtatactaaa
     2941 tatatttact atatctagta tacttactat attttaatct tttgatcgtc tattatatat
     3001 aatccgttcc tcgtcgttta aagtatcaac ataggcgtcc attgcaagtt tgactatttc
     3061 atacatagga atatcttttg cataactaag atcccgaatg gtttttagta cgggaggatc
     3121 tacttgaact ggttttcttt ctttcgcagt gtactttcgt tcccctaact cgacattaat
     3181 atcatcttta attgaaatat cttcagcacc aattttcttc ttatctttgt tcgttggtaa
     3241 taatgccatt tagtttaatc tcctttccaa gaggtaggat atgattggcc attgagtcag
     3301 catatttagg tgtataatca aatccttcaa tatcaccatg ttgttcatag tattcaattc
     3361 tttcttctaa ttctgtaaag atgtctgcaa aaatagccca ccatcttcga tctacataat
     3421 catttaattg gataccattc tcaccataca tttctaaccg atccgagcct ttaacaatgc
     3481 ttttaaaaat gtttgattca ccatacttat caattgtttt ttgatagttt gcgtgttgag
     3541 aggaacgtcg cactgagaaa agaacaggca aaattccaat gatttgaaga tgagactctt
     3601 caaaattcgt gacaatcgga actaaaactt tattaataaa atcttctgtt ccttccattg
     3661 cgtaccgttt taattcttgc attgcaataa tataatctgc agcagttaag aatgcacgaa
     3721 ccacattatc tgttgatggt gggcagtcga taaagatata atcgaaattt tttctcaatt
     3781 cttctaaagg tttaatgaag gccaatgatc tcttacgacg atcaggatat ttttcggaca
     3841 agtattcatt aagttcttca gatccaggag agccagccat aatatataaa tgtggactta
     3901 cttgagtaat acctgaaacc cagtcccctt ttttgattgc tgcagacatg gatcgatcaa
     3961 agctagtaat ttgatacgtt gatccaatgt tggaagtagt atttctttgt tggtcataat
     4021 caattagcaa cacgtctttt tcaaaaagtt gactagaaac aatcgctaat aaatcattgt
     4081 ctgtagtctt accaatgccg ccttttctac ctgtaagcat tacaactttt cctagtttat
     4141 ctgatgtttt aaatgcagtc cgtaagtttt ccatagttcc ctccaatttc ctatactaaa
     4201 tatatttagt atacttcata tacttaatat attcgatata tctaatatac tccaaattga
     4261 taagaattca gactttataa ggaatatagt ggatatacta aatatatttg ctatattcag
     4321 tatatcagat atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga
     4381 gtgaaaagag gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag
     4441 caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt
     4501 gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc
     4561 tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc
     4621 gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaacgt ttatttagtg
     4681 tacttaaatt aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa
     4741 gctaaactgt gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt
     4801 tattcagcta ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa
     4861 cctgcaggtc atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa
     4921 ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa
     4981 aagaaactaa cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat
     5041 cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt
     5101 gatatcgatt caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg
     5161 ggctacatgc caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta
     5221 aagacaccag gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa
     5281 atttcaaaaa caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt
     5341 aaccacttcg gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac
     5401 tatcagtatt cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa
     5461 acaaaagaag atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa
     5521 gtagatgaac cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga
     5581 cgtcttggtc gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat
     5641 gcattggata cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta
     5701 tctgaagcag aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca
     5761 agcagagaat tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat
     5821 gatctgttca ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa
     5881 tatagtcata aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt
     5941 gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc
     6001 aagcgaacac ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag
     6061 gtaacacgag gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg
     6121 gttattcaac taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt
     6181 ggactcaacg agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct
     6241 cttaaagaag tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc
     6301 cctatacaat atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta
     6361 ggtggtatac ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt
     6421 taaaaaatt
//
LOCUS       DASOUD010000118         6307 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.119, whole genome shotgun sequence.
ACCESSION   DASOUD010000118 DASOUD010000000
VERSION     DASOUD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.119"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            290..1135
                     /locus_tag="U4A29_002339"
     CDS             290..1135
                     /locus_tag="U4A29_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO0681445.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="U4A29_002340"
     CDS             1416..2636
                     /locus_tag="U4A29_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEO0681446.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="U4A29_002341"
     CDS             2633..5353
                     /locus_tag="U4A29_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO0681447.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="U4A29_002342"
     CDS             5337..6281
                     /locus_tag="U4A29_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO0681448.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2351 a          976 c         1218 g         1762 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaa
//
LOCUS       DASOUD010000119         6202 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.120, whole genome shotgun sequence.
ACCESSION   DASOUD010000119 DASOUD010000000
VERSION     DASOUD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.120"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="U4A29_002343"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="U4A29_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEO0681449.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="U4A29_002344"
     CDS             complement(2203..4389)
                     /locus_tag="U4A29_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO0681450.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="U4A29_002345"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="U4A29_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEO0681451.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="U4A29_002346"
     CDS             complement(4611..5051)
                     /locus_tag="U4A29_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO0681452.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="U4A29_002347"
     CDS             complement(5125..5664)
                     /locus_tag="U4A29_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEO0681453.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1611 a         1393 c         1093 g         2105 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga ag
//
LOCUS       DASOUD010000120         6185 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.121, whole genome shotgun sequence.
ACCESSION   DASOUD010000120 DASOUD010000000
VERSION     DASOUD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.121"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(151..1830)
                     /locus_tag="U4A29_002348"
     CDS             complement(151..1830)
                     /locus_tag="U4A29_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO0681454.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(1832..2044)
                     /locus_tag="U4A29_002349"
     CDS             complement(1832..2044)
                     /locus_tag="U4A29_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO0681455.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            2437..4167
                     /gene="efrA"
                     /locus_tag="U4A29_002350"
     CDS             2437..4167
                     /gene="efrA"
                     /locus_tag="U4A29_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEO0681456.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            4164..5924
                     /gene="efrB"
                     /locus_tag="U4A29_002351"
     CDS             4164..5924
                     /gene="efrB"
                     /locus_tag="U4A29_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEO0681457.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            6019..>6185
                     /locus_tag="U4A29_002352"
     CDS             6019..>6185
                     /locus_tag="U4A29_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681458.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILK"
BASE COUNT         1858 a         1107 c         1299 g         1921 t
ORIGIN      
        1 atttttttgt atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca
       61 aggaataaga acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca
      121 ggacaaggtg ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg
      181 caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat
      241 cgcttctttc aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg
      301 gaataagatt cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc
      361 acgagagaga atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac
      421 agctaataca agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat
      481 atcaccgatc ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg
      541 cgcagaatcg ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc
      601 aggaacacct acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg
      661 gacaggaata aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc
      721 accgtgaccg gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc
      781 agacagcaaa ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga
      841 aaagatcact gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg
      901 gctcaacgcg gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc
      961 aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc
     1021 taaacgttga ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg
     1081 accagttgcc acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct
     1141 ggcaaaaatg atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt
     1201 tttttctgat ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa
     1261 gactccttct tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt
     1321 gaaatcgaat ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat
     1381 ccctaatgca tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa
     1441 acggatgatc gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc
     1501 atctaatttg tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc
     1561 ttgtttcagc aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa
     1621 ggcttttact ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat
     1681 tccaagtaaa tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg
     1741 gaattgtacc ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc
     1801 tgtctcgtta tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat
     1861 tttctttttc gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg
     1921 gggtattttc ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag
     1981 attttgtttc ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga
     2041 tcattgtttt gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga
     2101 caacacaaaa cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga
     2161 ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta
     2221 ccttctgtat aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag
     2281 tatagaaatc aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa
     2341 tgactttttt atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa
     2401 tgaaaatgta acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc
     2461 atgcgttata agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt
     2521 gaattaggac ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat
     2581 tttgattatg tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt
     2641 ttgaatatct tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt
     2701 cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc
     2761 gtatcttcat tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa
     2821 aatattttac gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc
     2881 atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg
     2941 gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta
     3001 gataaaatca acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc
     3061 gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt
     3121 tcaaaaagct tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat
     3181 atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt
     3241 gtaggagatt tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta
     3301 tttgctagtg tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa
     3361 gccctagatg ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact
     3421 aaagggtata tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca
     3481 gtgatacgaa atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt
     3541 accggtagcg gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa
     3601 ggaaagatct tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat
     3661 aagattgggt ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg
     3721 cgatacggga aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa
     3781 gcttcagaat tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt
     3841 tccaattttt caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt
     3901 ccggaaatct atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag
     3961 ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt
     4021 gtgggaacga tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt
     4081 atggggacgc atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct
     4141 caattatcaa aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc
     4201 gatatatcag accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag
     4261 tggctttcaa cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta
     4321 atattgccaa tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca
     4381 cagttgttgg aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg
     4441 tcgttcaaag tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc
     4501 cggtttcata tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg
     4561 tagatgcagt cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg
     4621 gaattacgtt ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta
     4681 tgatcatgat tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat
     4741 attttcaaag catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga
     4801 caggttttag tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa
     4861 aagttaatca cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga
     4921 tgcctctagt tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct
     4981 tttatgttat tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt
     5041 ggcaaatcag tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat
     5101 cagcggcaac gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag
     5161 tagatgcttc actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg
     5221 catatgatcc gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa
     5281 ccgtagctgt ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc
     5341 gtttttatga cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga
     5401 gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag
     5461 gaacgattgc agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg
     5521 atgctgcgaa aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata
     5581 tggccatcaa ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg
     5641 cccgagcagt ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag
     5701 acacccgttt agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa
     5761 gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg
     5821 atcaaggtca gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt
     5881 atgaaaaact gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa
     5941 acagtttaaa acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag
     6001 aaaagtgagg acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga
     6061 gaagttgatc gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt
     6121 gaaaatgagt ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta
     6181 aaaaa
//
LOCUS       DASOUD010000121         6173 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.122, whole genome shotgun sequence.
ACCESSION   DASOUD010000121 DASOUD010000000
VERSION     DASOUD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.122"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            725..811
                     /locus_tag="U4A29_002353"
     CDS             725..811
                     /locus_tag="U4A29_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEO0681459.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="U4A29_002354"
     CDS             complement(932..1621)
                     /locus_tag="U4A29_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681460.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="U4A29_002355"
     CDS             complement(1628..1906)
                     /locus_tag="U4A29_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681461.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="U4A29_002356"
     CDS             complement(1866..2780)
                     /locus_tag="U4A29_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEO0681462.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="U4A29_002357"
     CDS             complement(2762..3142)
                     /locus_tag="U4A29_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEO0681463.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="U4A29_002358"
     CDS             complement(3592..3879)
                     /locus_tag="U4A29_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO0681464.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="U4A29_002359"
     CDS             complement(3899..4123)
                     /locus_tag="U4A29_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEO0681465.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="U4A29_002360"
     CDS             complement(4578..4748)
                     /locus_tag="U4A29_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681466.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="U4A29_002361"
     CDS             complement(4763..5296)
                     /locus_tag="U4A29_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO0681467.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="U4A29_002362"
     CDS             complement(5317..6159)
                     /locus_tag="U4A29_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEO0681468.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtcgatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DASOUD010000122         5959 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.123, whole genome shotgun sequence.
ACCESSION   DASOUD010000122 DASOUD010000000
VERSION     DASOUD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.123"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            43..113
                     /locus_tag="U4A29_002363"
     tRNA            43..113
                     /locus_tag="U4A29_002363"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Cys,seq:gca)
     gene            134..218
                     /locus_tag="U4A29_002364"
     tRNA            134..218
                     /locus_tag="U4A29_002364"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:168..170,aa:Leu,seq:caa)
     gene            280..352
                     /locus_tag="U4A29_002365"
     tRNA            280..352
                     /locus_tag="U4A29_002365"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:313..315,aa:His,seq:gtg)
     gene            362..433
                     /locus_tag="U4A29_002366"
     tRNA            362..433
                     /locus_tag="U4A29_002366"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:394..396,aa:Gln,seq:ttg)
     gene            451..535
                     /locus_tag="U4A29_002367"
     tRNA            451..535
                     /locus_tag="U4A29_002367"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:caa)
     gene            790..1665
                     /locus_tag="U4A29_002368"
     CDS             790..1665
                     /locus_tag="U4A29_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO0681469.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1769..2533
                     /locus_tag="U4A29_002369"
     CDS             1769..2533
                     /locus_tag="U4A29_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEO0681470.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2520..3962
                     /locus_tag="U4A29_002370"
     CDS             2520..3962
                     /locus_tag="U4A29_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO0681471.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3973..5304
                     /locus_tag="U4A29_002371"
     CDS             3973..5304
                     /locus_tag="U4A29_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEO0681472.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            5317..5775
                     /locus_tag="U4A29_002372"
     CDS             5317..5775
                     /locus_tag="U4A29_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO0681473.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1962 a          903 c         1262 g         1832 t
ORIGIN      
        1 ctataattta ttctttattt tatagattga taatagtata atggcggtat agccaagtgg
       61 taaggcagag gtctgcaaaa ccttcatcac cggttcaaat ccggttaccg ccttagtact
      121 aataaatcaa cttgccggcg tggcggaatt ggcagacgcg ctggactcaa aatccagtgc
      181 ccgttgaggg cgtgccggtt cgaccccggc cgccggtatt gactaagatg tcttcatctt
      241 agttttttat tgagaaatta aataacatct tttatgatgg cgaatgtggc gaagtggtta
      301 acgcaccgga ttgtggctcc ggcattcgtg ggttcgattc ccatcattcg ccctttattc
      361 ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt tggttcgaat
      421 ccagctaccc cagttattat ttttaaattt gccggcgtgg cggaattggc agacgcgctg
      481 gactcaaaat ccagtgtccg ttgaggacgt gccggttcga ccccggccgc cggtataatt
      541 atcaatatag caactttctt atagagagtt gctatatttt ttttgttttt atgtaattac
      601 ctccaaatca attcgataca agtattatca taaaacgagt aaaaaagata ttgataacgc
      661 ttttattatg tgttaaacta tttttgtaaa ttatgtaggg tgctactatt caattaggca
      721 cgacctaaga aagggttctt tcttaggtcg ttttttgtct ttaaaaattg tttggagaca
      781 aaaatatgta tgaaggagtc tgcaagttgt tttgttttat catatagagg aggtgaatta
      841 acggtgtatg tgatgaaaaa gctgaatcat aatgctgtat tgatcgatga cgatggaata
      901 gaaaaaattg cgatggggaa agggattggc ttttctgcta aagtgagtaa gccatttgat
      961 gttgaggatg ctgacaaatt atttgtatta gattctaaag aaaagatcaa acgattcagt
     1021 gagatagtct cgcagattcc aatcgaattt atggattttg cggaagaagt gattgaactg
     1081 ataacagcta ggatctcctc tccattagat tcaaatatct atattacatt gacagatcat
     1141 atttattttg ctattcaacg aaaaagagaa gatagagatg tcacagcaat tatgttgcca
     1201 gaaatgagat tcctttatcc aaatgaatat gatatagcag tagaagtggt cgagcgaatc
     1261 aatcatcgat atcaaaccga gttagggtca aatgaagtag gattcatcac tatgcatatc
     1321 attaatgctg agctaggaga gcctaatagt ttaaagagct taaaaatagt agaaatgacg
     1381 accacgattt tgtcccatct agaaaaatcc tattcaaacg tttatgataa agaatcactg
     1441 acttatcatc gcttaatggt tcatatcaaa ttcctggtta aacgcttgat ttatcaggaa
     1501 gaaacgccag tagaaaatat tggttttttt actgaaacat ttaaacatag caaagcgtat
     1561 tgtacagcac taaagattaa agatatcatc gaacagcaat ttcaacttgc cattcaagaa
     1621 aatgaaatca tctatttggc aattcatcta gcaagaatct attaaaataa gggcgtaact
     1681 atctataggc gcaaccaaat gacacatagt aaatggacta tgattatttg gttgcgccta
     1741 ttttagtaga aaaaagaagg agaatgaaat gggaaaagtg atttttaatt cggatgattt
     1801 tggctatagc catggtgtga attatgggat tatggatgct tatcagcgag gaattttgac
     1861 atcaactaca ttgatggcaa atatgccggg tttcgagcat gctgtgaaac taagaaagga
     1921 aatgcctagg ctaggtgtag gagtccattt aacattgacg tgtggcaaac cactcctcaa
     1981 aactgtcgat actcttatcg aaagcggaga gttcagatcc ttatcttatt atcagcatcc
     2041 atttaacatt gacaaagatc aactgtatca ggaatggaat gctcagattc aaaaaattta
     2101 tcatgcgggg atcataccaa ctcatttaga cagtcatcat catactcata ctttggatga
     2161 aaatcaggag gtagttgtta cattagcaaa aaaatatgat ttgccggttc ggagcaactt
     2221 tgaacgaaaa gatgaagttc gtcatgtcga ttattttgag ccttgtttcg atgtcgtagg
     2281 agaaactgat gaaaaaaaac gacaaatcaa tacgagtgtg gaagaatatt tggcaagttt
     2341 actcatgata ttgagagaag ataaaacgat cgaagtcatg tgtcacgcag cgtatttgga
     2401 tcaggaattg ttaaatggtt caagttttgt gtatcctcgt acccaccaag tcgaattttt
     2461 aattcattca gcatttgctc aaaaagtgaa aaatgatcct ttgattgagt tagtaacata
     2521 tgaggatatt taatagagga ggaataaaaa tgaatgttaa atcaaatgtg cagaagcttg
     2581 gaagagcgat gctacttcca gttgcagcga tgccattagc gggattgatc atgcgtttat
     2641 cagctgacga tatgctgaac atcccagtaa ttggtgcagc tggaaatgcc gtttttggaa
     2701 acttggatat cttatttgct ataggagttg caatcggttt tgtcaaaaat aaggacaaag
     2761 gaatacctgc acttactggt tttttagcga ttgctacctt gaaaaaaggc ttagaaatta
     2821 tggacccaga agtgaatatg gggatttttg gtggaattat ttccggttta gtagcggcat
     2881 ggacgtataa tcgctttaaa gaacaaaaat taccagtagt tttttcttat tttgcgggcg
     2941 aaaaattccc attgactatg gtgatgattc tacaaacaat cgcagctgtt tttttgggct
     3001 ttatctggcc gctcatccaa attgggattg ataattttgc aaaactgctc gttgatatgg
     3061 gagcattagg tgtggggata tttatgtttt tgaatcgttt gctgatcccc tttggactgc
     3121 accatgtgtt gaatacgtat atttattacg atttaggaag ctatacatct cctagcaagg
     3181 aagttttcag aggagagatg acacgcttta tcaacggaga tcctcaggct ggactgtttt
     3241 tatcaggttt ttttgtagta atgatgtttg gtataccagc tatatgtcta gctatctata
     3301 aagcagctta taaagaaaat aaagagatgg tcaaaggaat catgggaagc ggtgcaggaa
     3361 cttcatttat tgcaaatatc actgagcctg ttgaatttag tttcatgttt ctttctccta
     3421 tgttgtacgt gatccatgca ctttttgccg gtttagcagg gattgtctgt tacttattca
     3481 atatacgaat tggttttaca ttcggagcat gtattgttga ttatctcatc aattttcgaa
     3541 tcgctaccaa tgctattttg attcttccaa tcggaatttt cttttttgcc ttgtattacg
     3601 ttacttttta ttatttgatc aataagagaa acattcagac attaggacgt gaagctaagg
     3661 cagagtttgg caatgaagta accttagaag aaacagagtt agggttagca agtaaaaatt
     3721 atgaatatat ggcaacaaaa atgctacaag cgtttggagg aaaagccaat atcctggatg
     3781 tctatagttg taatacacgc ctacgggtag aggtagtgga tcccaccatg gtcgaggaac
     3841 agcgaatcaa gcaattaggg ataagcggga tcattaaacc aactgaaaaa aattatcaaa
     3901 ttatcatcgg cttagaagtg acttatgtta tggcagaatt caataaatta ttagaagaat
     3961 agaaaggaaa agatgatgat gaaaaaacag attatcacta tagctggagg cgggagtacg
     4021 tatacaccag gaatcgttca agccattttg aacaatgaaa aacggttgcc attatctgaa
     4081 atacgtttat atgatattga tgaagaaaga aatatggata tgtacttgat tgttaaattt
     4141 atgctgaaaa gaaaaggttt ttcaaatatt cgcattcgtg caacggatga tccaaagctt
     4201 gcttttacag gatgcgattt tgttttctca caaattcgtg ttggaggatt ggagatgcga
     4261 gaaaaggacg aaaagattcc acttaaacat ggattagtag gacaagaaac gtgtggactc
     4321 ggaggatttg cttatgggat gcgctcaatc aaaggtttat tggaaattgt cgatcatata
     4381 caagactatg cccccaaagc atggattttg aattatacaa atccagagtc gatcgtttct
     4441 gaagctgttc gtcgtaaata tccgaatgta aaaatgatta atgcctgtga tatgactatt
     4501 tcaattgaag aaacgattgc agtgaattac ggttatgatc gtaagaattg gatcgttacg
     4561 tattatggtt taaaccactt tggctggtat acatcgattt atgataaaga attgcaacga
     4621 gaaattatgc cagagattat tgaaaaacta ataacaaaag aaatgcaagt cgccgatttt
     4681 aatataggag ataaaacttg gcaaaaaacc tttcaaatga tgtcagtcat cacgaaaaat
     4741 tttccaagta atatcccgaa taattactta gagtattact tatatcctga tatggtcgtt
     4801 gagcatactg acaaagagta tacacgtgct aatatggtca tggatggacg agagaaaaat
     4861 accaaagaaa tggctgataa aatacgccgc ggtattgtgg aagaagtatt gaactttaat
     4921 tttggggagc atggacaata tatcgtcgat atagctattt ccttgttgaa tgatgatcgt
     4981 cgtcgcttta tgctaatcgt tcctaatcaa ggggcgattc cgaatctacg gtcagatgca
     5041 gttgttgaaa taccggcata tgttggtgca actggtgtgg agccaattac actaagaaaa
     5101 ccaatcagtg attttcataa aggattgatg gaggcacaag ttgcagtaga gaagttacta
     5161 gtagatgctt actttgaagg ttcttatcaa aaagctttac aagcatttac attgaatcaa
     5221 accgtaccaa atgccagagt cgcaaaaaaa atactagatg aaatgatcaa agtaaacaaa
     5281 aaatattggc cagcattaaa gtagaggtga taaaaagtgc atttctttaa aacaagaaac
     5341 attctaaaag ctgtagctag tggtcaaatt attccattaa cagcagtaaa agacgaggtc
     5401 ttttcttcaa aaatgatagg agttggatac gctataacga atcatgatgg atatatccat
     5461 gcgccagtta aaggaataat cgataatata tttcctaccc gacatgccat aacgatcgaa
     5521 agtgaatctg gcagaaaaat actgatccat atgggtttag atacagttga actcgaagga
     5581 agtccgtttc agatacgggt tgaaagtaac caggaggtaa aagaagggac actgttagct
     5641 attatagatt tgaagaagtt ggagcaagca caaaaagata cggtgatcat tgtagtttct
     5701 ccaggcattt ttgaaggaaa tttattaaaa caaaaccaag aagtaaagat taaagatagt
     5761 tgttttgaat tttagtgtac gatgaaaaaa gttaaatagg gtgatattta tgggtgaatt
     5821 tacaaaattt ttttgaaatg atatgtttca atcactagat ttaatcattt tgatttttgt
     5881 taattatcga atttttttgt tgctcctatg atttagaaaa cttattttca ggattcttaa
     5941 aaataaattt gattattta
//
LOCUS       DASOUD010000123         5903 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.124, whole genome shotgun sequence.
ACCESSION   DASOUD010000123 DASOUD010000000
VERSION     DASOUD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.124"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..1148)
                     /locus_tag="U4A29_002373"
     CDS             complement(54..1148)
                     /locus_tag="U4A29_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEO0681474.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1337..2098
                     /locus_tag="U4A29_002374"
     CDS             1337..2098
                     /locus_tag="U4A29_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO0681475.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEYAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2213..2653)
                     /gene="fabZ"
                     /locus_tag="U4A29_002375"
     CDS             complement(2213..2653)
                     /gene="fabZ"
                     /locus_tag="U4A29_002375"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO0681476.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2873..3628
                     /gene="fabI"
                     /locus_tag="U4A29_002376"
     CDS             2873..3628
                     /gene="fabI"
                     /locus_tag="U4A29_002376"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEO0681477.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3738..4751)
                     /locus_tag="U4A29_002377"
     CDS             complement(3738..4751)
                     /locus_tag="U4A29_002377"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO0681478.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4916..5836
                     /locus_tag="U4A29_002378"
     CDS             4916..5836
                     /locus_tag="U4A29_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO0681479.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1790 a         1178 c         1063 g         1872 t
ORIGIN      
        1 aagcagaaat aactggctat ttctagcgga ttatttctgc aataactaat tatctatttt
       61 ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa agtcaaggag gaataattcg
      121 tcatcggaga ttcttcctac aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg
      181 atttgtgttt ctcctttgta ttgaccgtac ccttcattgt caatcagtac atccccatga
      241 tgcatatctc gcgtattatg aggcgggaaa tctttatctt tgtagtatac acgagtcatc
      301 gttgaacgaa ggatatattc tgagcgatcc cctcgataac tatgaaggtt atcaaacaag
      361 cagattcgtt cgttttctgt aataccttct tccgtatcta ctttcaatgt tggataatcc
      421 gcgttaaatg cttctgccat ttctttcaat tcttcttcac ttgcgtacgc atttccaata
      481 gaaatatcat caatcaatcc tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga
      541 tctcggtggt cttccagcgt gcacaagcca tcttgcgtag gccaagggcc aaactcagca
      601 gattgcgaat tgacgaatgc catcgtattc aaattatatt ttctgaattt ttctgaacaa
      661 aaaacaaagt gttcgtatcc taaaccagag tatcgatgag gataaaaatt atgagaacct
      721 aataaattat ccgtgtttgg cgaataactc atgatgttgt caacgtaatt cgtgcccgaa
      781 ctcatattga tctcgatttt gataccaaaa ggattgcgcg tcatttttgc ttcttctgct
      841 cctgtgaatc cgatatctaa gcggaccccg taggcaccca tcttatggaa aaaagataag
      901 tcatcgtaag aaatctccaa ctgttcaaat aacgctggat tgatatcaac cattacttcc
      961 atgcctaggc tattcgcgta atcaactact tctttaaatt ctgaaagaac tttttcttta
     1021 tcatcattga tctgcagcaa actggtaaaa acccgtttat acccatactt atgagctaaa
     1081 tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg gataaatcga gattcctaat
     1141 tttcccatat taccatttcc tcctgcatct tttcgttttt gtatccactt acattatagc
     1201 aaacacaaag taaatggtct acaccttttc tttttctttt aaaataaatg ataaattcca
     1261 ataatttaag taggtatttt ccctctattt tgttatcatt aaagataaca aaatagtaaa
     1321 ttggaaagga acagttatga agaaaaataa agttatttta agtattagtt tagcagcagc
     1381 attgctgttt gctgctggtt gttctgcaaa caacacgact tcttctagtt cgtctacaga
     1441 aagttcaaaa agtacatcta ctgcggaaag cacgactgcc agtagcgtcg atttgaacgc
     1501 attggaatta ccacaattga cgaaagatgt cgccgaaaat gaagatctgg tcgaaatggt
     1561 gacgaataaa ggcacaatca aaatcaaatt atttcccgaa tacgcaccaa aagcggttga
     1621 aaactttatg acacatgcaa aagacggtta ctacaacgga ctgactttcc atcgcgtcat
     1681 caaagatttc atgatccaag gtggcgatcc taaaggcgat ggcactggag gggaaagcat
     1741 ttggggagaa ggatttgaaa cagagatcaa caaccacctt tataatataa gaggtgcgtt
     1801 atctatggct cgctcacaag atcctaacag taacggcagc cagttcttta tcgttcaaaa
     1861 tacagatgat atgcatgatg gtcttttaaa agacgactat cctcaagcaa tcatcgacgc
     1921 ttataaaaac ggcggttatc cttcactaga tggccattat actgtcttcg gccaagtgat
     1981 tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa acagatagtt cagacaaacc
     2041 aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa gaagcaaaac aaaaataaaa
     2101 aaataacgtc tgaatcctgc atagatgaca acgtatgcta ggattcaggc gtcttctttt
     2161 tacataaaaa gcaagtaagc ttccttttgt tttgctatat caggttttat agtcaagcat
     2221 tttggttgac gatgaacgtg aactcgcact cacagacttt tttatcatct acatatgctg
     2281 cagataacgc tgtaccgact gatcccttca ttttggtgat ttcaaaatga catttcagca
     2341 catctcctgg aacaactttt tgacggaatt tggccttatt gatcccaccg atataagctc
     2401 tttttcctag aaactcttct gattttagga taagtatcga acctgcttgt gccagtgttt
     2461 ctagaatcaa cgcacccggc atcactggat tgtcaggaaa gtgaccttgg aaaaaatctt
     2521 cattgattgt tacatttttt gtcgcaacga tcttttttcc tggttctaaa gtatctacat
     2581 aatcaatata acaaatagga taacgattag gaatcaattc cattacttca gtagcagata
     2641 atacgtaaga cataattaaa ctccttcttt caggcaattg ccattttcat tactttgact
     2701 atcaaagtat catattccta aatatttagc aatctttttt taatttaaga aaaaatagtt
     2761 tgacaatcaa aacattttga ctaataatta agaaatcaag aagttatttt agctattatt
     2821 ctctattttt ttacgactat caaattttta tcagaaacaa aggagagtca ttatgtcatt
     2881 tctagaagga aaaaaaatcg tcattatggg cgtcgctaac aaaaaaagca ttgcttgggg
     2941 ttgtgctgaa gcaatgttga aacaaggggc agaaattatc tatacttacc aaaatgaacg
     3001 aatgaaaaaa tcattagtca aattggtcgg cgaagaagca ttcactgtag aatgtgatgt
     3061 ttctagcgac gaaagcatcg aaaaagcaat gaatgccatc ggtgaatatg ctggggagat
     3121 ccacggcctt gtccatgcag ttgcttacgc caacaaagaa gaattgtccg gaaatgtgtc
     3181 tgatatttcc agagacggct atcaattagc tcaagatatc agcagctact ctttaattgc
     3241 tgtatcaaaa tacgcacaac cgtttcttgc accaaacagc gggatcgtta gcatgactta
     3301 tctaggttca gaacgcgcaa tcccgaatta taacatgatg ggtattgcga aagcttctct
     3361 tgaagctgct atccgttatt tggcagctga gttctcgcct aaaggaattc gggtcaatgg
     3421 aatctcagct ggggcaatca aaacattagc tgtcacaggt gttaaagact atcaaaaact
     3481 gatccagtta tctgaatctc gtacgcctga tcagcaaggt gtgacgatcg aagaagtcgg
     3541 taatgcttgt gctttcttaa tcagcccact ttccagcgga atcatcggtg atattatcta
     3601 cgttgacaaa ggtgtccatc tttcataaac ttttgttgca ttctttaaag taaaacatat
     3661 aaaaatcagg aaaaaacttt ttaaagtctc ttcctgattt tttcttttta cgtttttttc
     3721 aactcattac tttgaaatta ttccacgacc tcaatccagc cttctggtgc ttctatgtct
     3781 ccaaattgaa tacctgtcaa ttcatcatac aaccgttttg ttactggtcc tacttccgtt
     3841 tcactataaa atacatgaag attgttattg tgataaatcc caccgattgg agaaataaca
     3901 gccgctgtac cacatgcacc agcttctgca aattgatcta gatcatcaat gaaaatatct
     3961 ccttcaattg cttgcagtcc tagtcgttct tcagctaact gcaacaagga atatttcgtg
     4021 atacttggta agatagaggg cgattgcgga gtgatgaatt ttccgtcttt tgtaattcca
     4081 aagaaatttg ctgcacctac ttcttcgatt ttcgtatgtg tggctggatc aagataaaca
     4141 caatctgagt aatccagttc ttttgcttct gttcctggaa gtaaactcgc tgcgtaattt
     4201 ccgccgactt ttgctgcacc agtcccctta ggagcagcac gatcgtaatc agatacaata
     4261 aaattcgttg gttttagtcc acctttgaaa tacgctccta ctggcgtaca gaagacgaca
     4321 aataaatatt ctggagctgg atggacacca atgttttcac cgaccccaat cagtaaaggg
     4381 cgcaagtata atgtagcgcc tgttccatag ggcggcacaa aatcttcatt tgcttttacc
     4441 acttgcttga cagcatcaac gaacatcttt tcagggacca ttggcatcag caatcgtcta
     4501 gcactttgat tcatccgtcg actattttgt tcaggtcgaa agagattgac cttgccgtct
     4561 tttcgacgat aagcttttag accttcaaaa cattgctgac cataatgaag cgctggagag
     4621 ccttcatgaa tcgtcaggta attatcttct gtcaattctc cttcttccca ttgtccgtct
     4681 ttccatttgg caataaaacg ccaaggagtt ttgatataag aaaaacctag attgttccaa
     4741 tcgatttgca tatacttacc accttcgtta ttttctccac attttatcat tatccatatt
     4801 atttgtcatt ttattttcag cttttttcct tcattatagt tatttttgct atttcctttt
     4861 ttgaaaactt cttggtacaa tacagaaaga atcaaatttt agaaaagagg attttatgtt
     4921 ttcattatta gcaactgccg gtacgacaga ctcttctggc gagacattga cagatcaagc
     4981 tgtcagcaaa gtaaatgcgt ttcagcggtt ttgggacggg attgattggg acgccatttt
     5041 ggctgccttt atccaaaaaa gtttatatct gatttttttg atcgtccttt tttggattct
     5101 tcaccgtgtg ggaaaatatc tgattgataa gtcttttaat cagtattcta aaaaagccat
     5161 tctaaacgag agccgcttaa agactttaca tagtctttta aatactattt ttcattatac
     5221 ccttggtttc ttttttatct atgcaatttt atctgctatc ggtgtgccga tcggatcact
     5281 tttggctggt gccggaattg caggtgtggc aatcggtctt ggtgcccaag ggttcatgag
     5341 tgatgttatt accggatttt ttattatcat ggaacaacag atggatgttg gggactacat
     5401 caagctagca aacttaacga ttgaaggaac cgtcgtatct gttggtatcc gtacccttca
     5461 gctgaaagca acggatggta ctgtccattt catcccgaat cgaaacatca ccacgatcag
     5521 caatctctct cgtgcaaata tgcaagtact tttagatatt cggattgttc ctgaagaagg
     5581 atatgacaaa atctatgaga tcattgatcg agtaaatcgt acattagctg aaaaatacca
     5641 agaagagctc caaactgaac cttctatctt tggattagtg gatcttggaa acagcaattt
     5701 tgcgattcgt acggtctgct acgcattgaa cggaaaacaa tatgccttaa aagaagagtt
     5761 cttgagctcc tatgtcaaag aattgacagc ttctggtttc acgattccga acagtccaat
     5821 tgctgtcgga aaataatttg gacaaacgat gcaacgaatc tcactaaaaa gagcgtggga
     5881 caaaagtaaa gaacactttt gtc
//
LOCUS       DASOUD010000124         5877 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.125, whole genome shotgun sequence.
ACCESSION   DASOUD010000124 DASOUD010000000
VERSION     DASOUD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.125"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            641..1375
                     /locus_tag="U4A29_002379"
     CDS             641..1375
                     /locus_tag="U4A29_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO0681480.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="U4A29_002380"
     CDS             1391..2560
                     /locus_tag="U4A29_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEO0681481.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="U4A29_002381"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="U4A29_002381"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO0681482.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="U4A29_002382"
     CDS             3650..4576
                     /locus_tag="U4A29_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEO0681483.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="U4A29_002383"
     CDS             4595..5845
                     /locus_tag="U4A29_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0681484.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1204 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DASOUD010000125         5608 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.127, whole genome shotgun sequence.
ACCESSION   DASOUD010000125 DASOUD010000000
VERSION     DASOUD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.127"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..2106
                     /gene="acm"
                     /locus_tag="U4A29_002384"
     CDS             <1..2106
                     /gene="acm"
                     /locus_tag="U4A29_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEO0681485.1"
                     /translation="LIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKK
                     QNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNL
                     DSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGD
                     MLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRG
                     EEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENH
                     SEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFE
                     LRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKF
                     SINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAE
                     TKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNEN
                     KEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKY
                     RDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTERE
                     KIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVII
                     GLLIVTICAGIFLQKYKKV"
     gene            complement(2378..2863)
                     /locus_tag="U4A29_002385"
     CDS             complement(2378..2863)
                     /locus_tag="U4A29_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEO0681486.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    2953..3298
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3343..3984
                     /locus_tag="U4A29_002386"
     CDS             3343..3984
                     /locus_tag="U4A29_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO0681487.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4003..4638
                     /locus_tag="U4A29_002387"
     CDS             4003..4638
                     /locus_tag="U4A29_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO0681488.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4656..5471
                     /locus_tag="U4A29_002388"
     CDS             4656..5471
                     /locus_tag="U4A29_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO0681489.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1971 a          916 c         1157 g         1564 t
ORIGIN      
        1 ctgattcctg ttcatgtcta tgcggatgca ggcagagata tcagcagtaa tgtcacttcg
       61 ttgaccgtgg acccaacgaa tattacagat ggaggaaaca taaaagtaaa attctcgttt
      121 gacgaaaaaa aacaaaatat ccaaccaggt gattatcttt ggatcaattg gccaagtgaa
      181 ggaaatattc gcggtgaagg attccaaaaa gaaatcccat taatgattga aaataaaaat
      241 gttggaactt taacggtcag aaaagattct gcgcaggttg tgtttaatga aaatattaaa
      301 aacctagact cagtagaagg ttgggggcaa tttgagatac aggccagaaa cgtaaccgat
      361 accggtgaag aaaatacggg accattcacc gtgacgagcg gtgataaaac agctactgta
      421 aatgtgacaa agccagcttc tggttcttct tctagcgttt tttattataa aacaggagac
      481 atgctacctg aagatactaa gcacattcga tggtttttga atattaacaa taacggcact
      541 tatgttgaac aaccagttaa aatcagtgat gaaatccaaa gtggacaaag attagatcca
      601 agtacgtttg aaattaatca aatacactta ggtgaacaga aggtttatag aggagaagag
      661 ggaatccaac aatttttaca agatttcccc agcgctacat ttaatttttc agtaactgat
      721 aattacattg aaataactat accaaaaaac tttgtcaatc ttaggaaaat aatggtgagt
      781 tataagacga tcatagaaaa cccagaacaa ataaattttg agaatcattc ggaagcttgg
      841 tttaaagaat ttaataaacc tgctgttgat ggagaaagtt tcaatcatac agttaaaaat
      901 atttcagctt ctggtggagt caacggtaca gttagaggcg aattgaagat tttcaaatat
      961 atcaatgaca cagagattgg tatcccaaat gtgactttcg aattaagacg agcagatgaa
     1021 cagcctattc aaggacagtc tagtatcctg ttgacttcga atgaacaggg agaagctagt
     1081 atcaaaggcc tgcaagtagg agactatgtt gtaaaagaaa aagaagcacc caattggatt
     1141 gattttgatc cactaagttc aaatgaactc aaattctcca taaacgaaaa tgatacagag
     1201 ggagtcagtt tacctattta taacaagaaa aaggttacga acatcacagc aactaaaatt
     1261 tggaacggtg gaaccactcc aagaccgagt atttatttta aattattcag agcaagtaca
     1321 aacaattggg aaccagttcc tgatgcagaa acaaaacgat tagacaatgg aataacttca
     1381 gtgacttggg aagatatcca acagtatgat gatagtggaa acgaatatac gtttaaagtg
     1441 caagaagtag atcaaaacgg aaatgattat gtcccttcag gttatcaaaa aattgaaaac
     1501 gggctagtcg ttacaaatga gaataaagaa gtcatttcag tctcaggaca aaaaacatgg
     1561 gaagacaatg agaaccaaga cggcaaacga ccgacgacga tcacagtaaa tctattagcc
     1621 gacggtcaac cgatccaaca caaagaagta agtgaaaaag atgattggcg ctatcgtttc
     1681 acaaatctgc caaaatacag agacgggcaa gaaatcatct atactgtaac ggaagacaac
     1741 gtgcccgaat acagtacgac gattgacggg tataacatca aaaacagtta tacaccggaa
     1801 aaaacaaata tatctatacc aggtagccat ataacaccgt ggaaaaattt tccagagaag
     1861 acagagagag agaaaattag aaatctattc tctcaaaaat tgcaaatatt tgctcataca
     1921 ggagaatcca gcgaacaaag aagcaattat attagtaaga gagctgtacc taaacagata
     1981 acaaataaat caaaaagcat tcttcctaaa acaagtagta aaaaatctag ttttgctgta
     2041 atcattggtt tattgatagt aactatctgt gctgggatat tcttacaaaa atataaaaaa
     2101 gtttagttag aatataatat tttttgttaa atatgactga aatcaaaaga ataaaattcc
     2161 gatttataat cgaaaaaaga agcattttta tatatgcttc ttttttcgta gaatcaataa
     2221 atatggattt ggctaactat ggaagaatac tcgtattagt tttcagcgtt catactagtt
     2281 gttacattgt ttgattggcc aaaagaattt ctgatatatt ttgtgctaaa taaagaaaaa
     2341 gacgcttgaa ttgggaaata agaaaactac tctatcatta caaattttca taaactggca
     2401 gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa atccataatc aacgtagtgg
     2461 tattgatatt tggatgaaag cctacggtat cttgacgatc aatagcagca tcgatcacga
     2521 cttggatttt tttctctttg tcgtgcatca atgctaatgg agtcactgta cctgctggaa
     2581 cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga taagcgcttt tcttttaatt
     2641 gttcagctag ttgttttaaa tcggcgtgct tttcatcagg caagatgaca agatagattt
     2701 gttttccttt tcttgatttc aataatagat tttttacttg cggaccaggt agggaaaaag
     2761 ggacattttt gactgacgta atcgcaggat gatcgacttt ttgaaaagag atacccagtt
     2821 tttttagtag ttcataagct tcttgttcgg ttgcacgact catcgcgcat caccctcctt
     2881 tttttattag tgtatcataa aaaagagttg acaaatgaaa aataatcatg taaattacac
     2941 gtaaagttaa aacaaatgac atcgaaaaga agagtagccg ttaggtgttt ttagagagtc
     3001 tctggttggt ggaaagaggc agcagttaac cgtgaaacac atcttggagt cgattttctg
     3061 aagcgtatag taggaaaatc cggggagccc gttacagctg cggtcgttgc tagatgacca
     3121 gataaggctg gcttttccgt gaggagtcgg taaaaaaagg tggaaccacg tgattaacgt
     3181 cctttgatct tcggatcaaa ggactttttg ttttcatctg aacggcaacg actgtgtgag
     3241 gttttttctg caaggaaaaa ataaacagag gtggaaccac gtataatacg tcctcttggc
     3301 atttgccaag aggatttttt tattttaaag gaggaaaaga agatggagta tttgattgaa
     3361 gttatgccgc aattattagt aggagcactc acaacattga aattattttt atttaccttg
     3421 attggctcat taccattagg gattgtttta gcattaggat tgtctttgca ttttaagccg
     3481 ctacgctact tgcttcaaac atatgtatgg gttatgcggg gaacaccgct gttgttgcaa
     3541 ctgattttta ttttttatgg gttgccgacg attgggatcg tttttgatcg tttcgaagca
     3601 gcactcattg cctttatctt aaactatgca gcttattttg cagagatctt tcgtggaggg
     3661 atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag ttttgcaatt aagtcccttt
     3721 caaacggtga cgaaaatcgt catcccacaa gtgacaaaga ttgttttacc ttctgtaggg
     3781 aatgaagtta tcaacttggt caaagattct tccttgatct atatattagg tttaggtgac
     3841 gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga ctttactgcc tttgctaggc
     3901 gtggcaatca tctatcttac attgactggt attctaacaa tcgtaatgaa acaagttgaa
     3961 ggaaaattaa attattaccg ctaactgata ggaggaagaa atatgattga attgaagaaa
     4021 gtgaacaaag catttggtga aaaaaaaata atcaaagatt tgaatctaat cattccagat
     4081 gggaaaacat tagctatcgt aggtccttcc ggtggcggta aaaccacgct tttacgtatc
     4141 ttggctggct tagaaagttc tgacagcgga gaattttatt tagacggtgt accgttcaac
     4201 ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg tattccaaga cttccagcta
     4261 tttccacatc tgaatatttt tgaaaacatt accttagcac caaaaatggt attgaaacag
     4321 acaaaagaag aatacaccaa aaaagcagaa gaattagcgg cttcattagg gattagtgat
     4381 ttattgaata tctatcccta tcagctttct ggcggacaaa aacagcgggt agcgattgcc
     4441 agagcattgg ctatgaatcc acgggtattg gcttatgatg aaccaacaag tgctttagat
     4501 cctgaattac gccagcaagt cgagcgtttg atcctatcat tgaaagaaga tggggtaacc
     4561 caaattattg tcacccatga tttagtattt gcagaaaatg tcggtgatca attactaaaa
     4621 gtttccccaa ttcattaaga aaggagaaga tagagatgaa aaaaaccagt tttctattcg
     4681 ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa caaaaaaact tcagcagata
     4741 attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg tttagacgat acatttgttc
     4801 ctatgggatt tagagataaa gacgggaagc tcacaggatt tgatattgat ttagccaaag
     4861 cagtctttga acaatatggg attacggtag actttcagcc cattgactgg tcaatgaagg
     4921 aatttgaact gaacaatgga acgatcgatt tgatatggaa cggctattcc aaaacgccgg
     4981 caagagaaaa gaaagtgcaa tttacaaaac cttatatgga aaacgatcaa gtgctgatca
     5041 cacctaaaga atcaggaatc acaaactttg gacaaatgaa taataagaga ttaggtgcgc
     5101 aaaatgggtc atctggttat gatgtattta cgaaacaacc agaagtatta aaagagatag
     5161 tcaaaaacca agatgctgtt ttgtatgata gttttaatga agcattgatc gatctgaaaa
     5221 gcagacgggt cgatggtctg ctgatggata aagtatacgc agactattat ttgaaacaaa
     5281 gaaacgaact agaagacttc aacataacaa aaggttctta tgagagtgag gattttgcag
     5341 ttggtgcacg caagtcagat agtacattag tgaaaaaaat caatgaagcc ctagatcaac
     5401 tagagaaaac aggtgaattc caaaaaatat ccgataaatg gtttggcgaa gatgttacgc
     5461 cgagaaatta acaaataggg gactgtgaca aaagacgtgt cacagtccct ttttccgaat
     5521 aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg aatggcaggc tcacaacctc
     5581 ttggtaaacg gtgttcaaaa gccagttt
//
LOCUS       DASOUD010000126         5592 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.128, whole genome shotgun sequence.
ACCESSION   DASOUD010000126 DASOUD010000000
VERSION     DASOUD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.128"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            3..860
                     /locus_tag="U4A29_002389"
     CDS             3..860
                     /locus_tag="U4A29_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEO0681490.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQRRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            882..1391
                     /locus_tag="U4A29_002390"
     CDS             882..1391
                     /locus_tag="U4A29_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0681491.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1428..1829
                     /locus_tag="U4A29_002391"
     CDS             1428..1829
                     /locus_tag="U4A29_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681492.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1941..2294
                     /locus_tag="U4A29_002392"
     CDS             1941..2294
                     /locus_tag="U4A29_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681493.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2328..2771
                     /locus_tag="U4A29_002393"
     CDS             2328..2771
                     /locus_tag="U4A29_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEO0681494.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2788..3873
                     /gene="uxuA"
                     /locus_tag="U4A29_002394"
     CDS             2788..3873
                     /gene="uxuA"
                     /locus_tag="U4A29_002394"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO0681495.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3866..4579
                     /locus_tag="U4A29_002395"
     CDS             3866..4579
                     /locus_tag="U4A29_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO0681496.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4786..4935)
                     /locus_tag="U4A29_002396"
     CDS             complement(4786..4935)
                     /locus_tag="U4A29_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681497.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            5331..5531
                     /locus_tag="U4A29_002397"
     CDS             5331..5531
                     /locus_tag="U4A29_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO0681498.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1940 a          856 c         1214 g         1582 t
ORIGIN      
        1 aaatggctga aataatggat aatcaaacag gtgctcctga agaaatcacg aaaaaagatg
       61 taacaaaagc ctatctgaga tggcattttg caaacgaaat tcctcactct tttgaacgtt
      121 acttggcgcc atcactacta tacggaatga tgccaatctt gaaaaaactt tataaagatg
      181 aagactcttt aaaagcagca tataaacggc agttactgtt cttcaacact caattaagct
      241 ggggtggcgg tgtgattact ggcctaatgt cttctatgga acagcgaaga gcagaagaag
      301 agtatgaaga aaaagagatc ttgatgcagg atgacttgat gtataacacg aaagcgggat
      361 tgatgggggc actagcagga atcggagatg caatcgattc aggaaccgtc caatatattt
      421 ttatcgcaat cgcggtacct tgggcacaac agggaagtcc tttaggcgca ctttttcctt
      481 ttatctgctt tgggttgtat caggttttac taggggtatt tttcgcaaga caagctttca
      541 ctatgggccg taatgctaca ggcctaatgc aaaacactgg tgttcagaca gcgattgaaa
      601 tgctatcagt tttagggttg tttatgatgg gggtgcttgc gggaaactat gtaaaagtag
      661 aatcttcatt gaaatttgcg atatccggac gtgagtttgt tatccaggaa attcttgatc
      721 aaatcgtgcc agggatatta cctttagctg tcgtaatggg tgtgtacttg ttctatacga
      781 aaaaaggatt gaaagtcaca caagcattat tatggctgac tggaatatta attgtgctag
      841 caggaatcgg tattttataa aaagaaggga acgtgaataa gatgggtgta gtcaatttag
      901 cgcgtgtgga tgagcgtttg atccatgggc aagtaatggt aacgttatct caaaaaagtg
      961 gagtgaattc catttttgtt gtagacgaag ttgtagcaaa agataaattt atgagagatc
     1021 tttacaaaaa tgcaggaagc cgtacaggac aaaaaacaat tgtcattaca ccagagaaag
     1081 ctaaattcta ttgggatgaa tatcaattta aagaatacaa ttgtattttg atcgcaaaaa
     1141 cagtccaaac gatttacgac ttagtaaaac acggaatccc catggaagaa ttgaatattg
     1201 gagggattgc tcaaaaaaat cctgatacag atcttttagt gacgaaatct gtttatttaa
     1261 ataaatcaga tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta aagaatattt
     1321 attttcaagc cactccatct gcacctaaaa caggattaaa agatgtattg aacaagtttg
     1381 atttgtcata aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg
     1441 agctgtttcg tgattgggaa tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa
     1501 agagtaaaca gcgttttctt tcagcattag tatcagatat ttacagtatg agaacagatg
     1561 tgacagtcat tgcttacgat acgctagcct accgatcaaa aaatatttat gttggagata
     1621 tcgaaaaagc agagaaagtt atctgtactt attacgatac acccgttcat gcattaggaa
     1681 gttatttcat gtttgattgg aaagaccaaa ggaaaaaaac aatctactct atattattgt
     1741 ccttcatttt gttgttcagt ttaggttggt ggggaatgat gatatataac aaaaatcctc
     1801 atcacgtatt cgatctgcta tccgtataaa caggtatcac tgtactagca tttgggagct
     1861 actttttctt attgggaaaa gcagctcgcg gatggcctag cagacaaact tttattcgaa
     1921 atacttcatc catattaacg atgctagaaa tgatccgtac gattgatgat ccgagtgtag
     1981 cgtatgcgtt tgttgatgaa ggctgttatg gagaaaaggg cttagacagc gtgagatcgg
     2041 gtatgaaaaa agaggctatt cttttttatc tagactctgt tggggcagat acaccgctac
     2101 agttttcggg aaattatttt tcaaataaag aacagtggct gaaacaagtt gataagttga
     2161 aagaaaaaaa cgtcaattat atttttagtg caagaaaaaa gcaagcgcag ttcttctatt
     2221 tgaccaaaac agatttacga gggaaaacat ttaattggca aaatgccaat cagattattg
     2281 ctctttttcg ataagtaaaa agtgcaaaac aaggagtgag aagactaatg ttggaaatca
     2341 tgatcgttac acatggtaag ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg
     2401 ggacatcgag ccatattgaa acaatgtgct tagagtctgg ggaggacatc caaacattcg
     2461 gagagaaggt caaagaaaaa ttaactatga tcaacacagc agaacccatg ctagtatttg
     2521 ttgatctaat tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat gaactgactg
     2581 aagaaaagaa agcagccact tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag
     2641 caatcaatca tcagatgctt cacacaccga tagaagaagc ggtggaacaa attgtcaaac
     2701 aaggaaaaga taatttaact gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg
     2761 acgattttta gaaggaaggc tgaaaagatg aagtggggat ttagatggta tggtgaaaaa
     2821 ggggattcga tcccattaaa atatgttcgc caaataccgg gaataaatgg gataattgga
     2881 actttgctga ctaaaatgcc tggagatgtt tgggagattt cagaaattga ggagttgaaa
     2941 gcatctgttg agagccaagg actggaactg cttggaattg aaagtgtagc gatccacgat
     3001 gcaatcaagg cagggacaga tgaacgagat tactatattg ataactatat acgaacaatc
     3061 aaaaacttgt ctcgatgtgg ggttcgattg atttgttata gcttcaaacc aatttttgga
     3121 tgggcgaaaa ctgatttgtt taatgaaaac aacgatggaa gctattctct tttatacgat
     3181 cagaaaatcg tcgatcagat ggagccagga gaaatgtatc aattgataca cagccagtca
     3241 aagggattca aattgccggg ttgggaagaa gaacggttga aaaaattcgc tcagctggaa
     3301 aaactgtatg aaggaatgac cgaagaagaa ttatttgaaa atctgactta ttttttagaa
     3361 aaagtgattc cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc agatgatccg
     3421 ccatgggaac tttttggttt tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt
     3481 ctaaatgcag tagattctcc atataatgga ctaacgctgt gcacaggatc tttaggggca
     3541 aatccggaaa acgatttagt tgaaatcatc catgaggtag gttcgcgcat ccattttgtc
     3601 cacttcagaa acgtggagtt tttaggagaa agaaagttca aagaaacggc acatccctct
     3661 acagaaggct cccttgatat gtatcatatt atgaaagcgt tggtgcaaga agggttcgac
     3721 ggtcctatac gccctgatca tggtagaacc gtgtggggtg aagtggctat gcctggatat
     3781 ggcctgtatg atcgtgcaat gggactcact tacatgcaag gtctatatga ggcgataagt
     3841 aaggaaaaac cactgggaga gcattatgaa taaaatcaag ctcttcatct ttgatatgga
     3901 tggcctttta ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa
     3961 gtatgattat gaaatgcgta aggaagtcta ttatttacta actggccaaa cagaaatggc
     4021 gataagagaa caaatgggca ttttgtacgg agaagatgtt ccatatatca aatggcgaga
     4081 agcaatcaat caatataaag aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa
     4141 aggagcagaa gaaattttat cttttgcaaa agaaagagga atccatacaa tcgtggcttc
     4201 gtctaataca cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat atgccttgtt
     4261 tgatcaaatc atttcagggg atgatgtaaa aaaaggaaag ccggaaccag agatcttttt
     4321 gaaagcttgt tcaaaaatga atattcctcc aagtgaagca cttgtatttg aagactcgat
     4381 tgcagggatc gaagcagcaa ggcgagcggg cattttgtct tttctcatca aagatcattt
     4441 gatagggttg cccgatcata atgggaagca taagttgaaa gtagatgtaa gtttatatga
     4501 atccaataaa gcagactatg aattttatga cctgcatcaa gcaaaacgat ttttagaaag
     4561 taaagggttg tatctctaat aaagaggact attttctagt cctctttatt tattgtcctg
     4621 cacgactgag ggaacagatg aaaaagaagt aattttttat tttgaagtat ctaccttatt
     4681 tgtagtgaac aatttagaac aaacacaaaa cgcatctact gttatctacc agtagatgcg
     4741 tttaaagaga agaacgagaa tcgtccatat gagggtttcc actccttatg cagatttttt
     4801 taattcttgc actgcttgtt cgatcgtaac acctgtcgct acatgattgg tattttcttt
     4861 gtcaaaaacg acaaatagat tcaaatctgt atccaaggtt actcgatatt gtatatcttt
     4921 gttatagaat gtcatagcca ttctcctttc tttaaatact tgctgaaagg caacgaacgc
     4981 gttgataacg aaattccatt tataagtata ccaccaattg aaaggttttc atagttaaga
     5041 aattttaaag tcaaaagttg cagataaaag caaataaaca agaaaacaaa aagtgaatca
     5101 atcgcggtta ttcaactttt tcttcgattc gaagacaata gaagacaatg aattttatat
     5161 ttcattgcat tatccttgtg ttattagaaa aagcatgata tattaaatga gtaatctatt
     5221 ttgaaacgta atttgagcga tatatctaca tagataaaac tccttttgcc tatcaaatat
     5281 ttattgcaac gaaaaaacgt acattgatac gtatcaggag gaatttattt atgaataacg
     5341 gtacagtaaa atggttcaac gcagacaaag gttttggctt tatcactggt gaagatggaa
     5401 atgatgtatt tgcacatttt tcagctatcc aaggtgacgg cttcaaaact ttagaagaag
     5461 gccaagcagt gtcttacgat gttgaagatg gtcaacgtgg ccctcaagca acaaacattg
     5521 ttaaagctta ataaaaattg acttttaacc gtcttttcga aagaaaagac ggtttttttg
     5581 tacgaaaaaa tt
//
LOCUS       DASOUD010000127         5569 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.129, whole genome shotgun sequence.
ACCESSION   DASOUD010000127 DASOUD010000000
VERSION     DASOUD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.129"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(205..660)
                     /locus_tag="U4A29_002398"
     CDS             complement(205..660)
                     /locus_tag="U4A29_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681499.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1280..1696)
                     /locus_tag="U4A29_002399"
     CDS             complement(1280..1696)
                     /locus_tag="U4A29_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681500.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1698..1979)
                     /locus_tag="U4A29_002400"
     CDS             complement(1698..1979)
                     /locus_tag="U4A29_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681501.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2355..2879)
                     /locus_tag="U4A29_002401"
     CDS             complement(2355..2879)
                     /locus_tag="U4A29_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO0681502.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            complement(2896..3864)
                     /locus_tag="U4A29_002402"
     CDS             complement(2896..3864)
                     /locus_tag="U4A29_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEO0681503.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(5031..5186)
                     /locus_tag="U4A29_002403"
     CDS             complement(5031..5186)
                     /locus_tag="U4A29_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681504.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(5205..>5569)
                     /locus_tag="U4A29_002404"
     CDS             complement(5205..>5569)
                     /locus_tag="U4A29_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO0681505.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1675 a          907 c          942 g         2045 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgattttta attggtctag ctagttacta gcttctaatg cttgatttta cgcgatttct
      901 cttttttagt tctttgtgca tttttatctg tcatttttta aagacttgtt atttttgaga
      961 ggatttgata tgcttttttt gtctttctct agttcttcac tttgtaatat ttttatgttg
     1021 atgaatagga taatctcatt ttgggctgct cgtgagagca gtcttttttt gtttcagggt
     1081 acgtcctatt ctttggattt ccgcgctatt aaataaccga tacttgggga ttggggtagc
     1141 gccccaaaat acaaaaagaa gaagttgcat aagcggattt ttcttttttt tcgcaagttg
     1201 gtaccaactt gcaaatcttg ctggaagact gatccctaaa aagaaatcga ggaatttttt
     1261 tagggatcgg tttctttttt tatcttttta gaaagtttgt tgggtcaaaa tttccagaag
     1321 taaaggtttg attggtttct ttttgacgat ttttgggttg atttcgtcgg gaaggattga
     1381 gttgattttc aattttttct ttcacttctt gatacacttt agaatttgta ccgataagaa
     1441 caccagtatc ttttagatat aaatcagcaa attctgccat gatttctgtc atgatagaaa
     1501 tttcttttcc catggcattg agttttgctt ggttactggt tgtggttgta ttggtatcag
     1561 gttcataact catacataaa aaacgaatgg cttctgaata attgttgacg tctggatttt
     1621 tttcgatgat ggaatctaaa acgtcaacgt gactgggata aagggttacg tgtttttgaa
     1681 cagaagtttt tctcatgtta tattcctccg ataagtgttt caatgagttg gttgtttgtg
     1741 tgaacatagt cattcagtat gttcgtattt tctgtttgaa tggcaataaa tttttcgagt
     1801 aataaatcga attgatcttt tttgtaattc ataagttgac ggtcaaaatc actttcgatt
     1861 agaactttta gatatttatt tcgactccat tttttgcccg ttttggttgt gagttcctga
     1921 caacgtttat ctattcttgc aataattttg ggattgatat ttcgaactaa aatgtccatg
     1981 gaaattttcc tttcgtatca gcgtataatt tatgtatacc atacaaatat agtatttgta
     2041 tagctatttt tcgccaaaac actatacata gcctagccac aatggcaaaa aagtgatagc
     2101 aaattttttt ctgagaaacg tgataggcac agcctatcta gcacctttcg taagaaagtg
     2161 ataccacgtt tccttatgct ctttcaaaat tcagaaaaat cagctgtaaa acgagtgcag
     2221 tccgagtaag attcaacgaa aaaacgggga ttggggcgcc accaaaatat cgcaacatgg
     2281 gtccatgttg ctctgctcgc aaaagcaaaa agaagaataa aaccagcaat ttttattaat
     2341 ttaaattgct ggttttattg ccagaattgc caccattttt tctccgcctt tgattgtttt
     2401 tctatcgatt ctgtttcttc agaaagagtc gttgatttat tttccgaaga ttcttccgta
     2461 taagtcaact gtagttgttc ctgcagtttc tcgatttgtt ggttggcttg cagggtgagt
     2521 tgttgttggt ggtcgagtaa tttttgcatt tgttttattt gctcgtcttt ttcttgtagt
     2581 tgcatatctt tttgatttaa ctgttgttct aggagagaaa ggacttctaa ttcgggaact
     2641 tggttggcgt ttttgtggtt ttcggttttc gttttttcgc caactagttg ttgatttgag
     2701 ttttcgtttt cgttaaaaaa cattgatttt atagcctttt gtccggtttc attaatcaca
     2761 aacttgtttc cgattttcga aaaccaaagt tttcgatttt cgtcagaaac ttttttgtta
     2821 attgctgttt tactaactcc taattcttca gcaagttctt tgattgtttt ttctgtcata
     2881 aaatctaaaa acccctcaat ctttgaataa gttttgaaga ttgtctaata agtctttttt
     2941 tagttctgaa ggaatatcgg aatttatctc tgatttttgt gcattatttt ctttattttg
     3001 ggtattttgt tctctttcta acttttctaa ctgttgtaaa agttccaatt ttcgcttttg
     3061 ttctttgatt ttttcttgta atgaagtagg tgctttttca gattgaactt gttggttagc
     3121 tagttctaat gctttgtttt gttcttcttt tgttggggca tgtttgttaa agtcgtctga
     3181 tttttttatc tcaggtttaa atgtaaattg atagccaatg acaggctttc cccgtccttt
     3241 tccgtatttc tttttgattg ttaaaccttt aaagattgcg gttagttctt gtcgtattgg
     3301 attgaacaca cgttgatcaa tcgtaccttg gttgtaactt ttaggaatat caagtaattc
     3361 atgaaagtct tcttttgaaa aataagcgaa acctttggtt ctgtattgtt ttagtaatcg
     3421 aaacattgtt tttgaataac tgctttctaa ttcattgaat tgttggagag aatagcgcac
     3481 ccattcttca agattattaa gtaagggtaa ggctttttca tgaacttgta tttcagcaaa
     3541 aggaatatca gattttccgt cgattttaaa ttgattaaat aaaacaaaac gttcacgttg
     3601 caaaccgtca gcacttcttc taccaaaacg taaatccatt aatttgtcat acgttgtttc
     3661 taaatcatct atgaatcgaa catttgcagt tgctttataa ttgcttaaat cttttaattg
     3721 ctcgaatgta aattgtactt ttttatcacc tacatcacgc attcttgata cgatagaaaa
     3781 gaacaaattc atttcgactg gtgtaaattt tcgcaacgga attgtattta attcatgatg
     3841 atactttacg atttcattag acatatttaa aatagctcct ttcttactcg cacaagagat
     3901 acacgttatg aaatacaaaa tgcctgttta tctacatctt atattaacat cgtcgatgta
     3961 ctaattcaat gtacaaacag acaaaacgat gtagataaac agacaaaacg atgtagataa
     4021 acagacaaaa cgatgtagat aaacagacaa aacgatgtag ataaaagcgt cattcccttg
     4081 ggagagtaag gatatagccg acggtaaaga ggtttaaaaa gaggtttaaa aagagattat
     4141 aaaagagggg gacaaaattt cccgcctaaa aaaccacttc ttttttctcc aaacagattc
     4201 gcgcattttt ctcaactggg aatcttactc cctacagtcg cttaaaaagg tgtgatcgct
     4261 ctgctctcac attatactgc gcagtcgctt ccggctcctt tgctaaaaat gttcataagg
     4321 aactctttga gaagctatga gccttcgttt gagacgtttt aagacagtct tagtataaat
     4381 cccttatctt gttcttaaag cgccttacag gcggtcaggg caagccctaa caaccctaaa
     4441 gttgtctgaa tgccctctaa aattcgattt aagcactctt aaacctactc ttatatattt
     4501 atatacttag acgtttcaaa cgtcttaaaa cgcaaattag agcttgataa agatatttgc
     4561 tcgtgtgccg acttttttaa aagtctaggc acacgccatg acaaagctga aataaactgc
     4621 ttcagctttg tcatggcgga ttcgctcact tgaaaaagtg ttcatttcgc tcgattatta
     4681 gcatatattt tttaactttt tttgctagcc aaattttgca ttagttcgct cataaatccc
     4741 accctttcag tacgtcctac ggacgtatcg ttcgtaatgt gaaagtacct ttaaaggtac
     4801 tttctacaaa actcactaaa gtgtttggca agctctatat ttgctagcca aatacctacg
     4861 tatttggtgc aaataaccgc ttgtcaaagg ggcgcaagct cccccttgaa tcccccctca
     4921 aaacccacgc tccacaatac aaaaaaggct agcaaatgct agccactttt tgtaaagttt
     4981 cgcttgtttc accttcggtg aaattgttgc tttgcaacga tcaggtagag ctacattttt
     5041 tttcgttgtt caaatattct tacgccattt actattaaaa tcgtaggtat tccaaagtaa
     5101 agaaaaaatt gattagcagg taatacaaaa tataaaataa taccagcaat aattaccaaa
     5161 cttatagtaa gttttgttaa tttcatgaag tatgccacct tatcttatat agatatcaaa
     5221 tagattaata tgccgattgt attataaaat ccatgagcaa ctatggaata ccatatattt
     5281 tttgtttgta tataaacaaa acttaaaata ataccaataa agaaaatact taggcctccc
     5341 ccatgaacaa tagaaaacag aaaagaagaa attagtattg aaatatattg ttgttttttg
     5401 tttgcttgag gatcaatcat acaaaatcga aaaattcctt cttctaaaaa agctccataa
     5461 gtagcagcat ttaaaaagaa taaaaggaga gaaccattta cttcattagg atttatacct
     5521 tctgaaaaag atgtatttag aatattgatt aggaataaaa cagagaatg
//
LOCUS       DASOUD010000128         5412 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.130, whole genome shotgun sequence.
ACCESSION   DASOUD010000128 DASOUD010000000
VERSION     DASOUD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.130"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(72..542)
                     /locus_tag="U4A29_002405"
     CDS             complement(72..542)
                     /locus_tag="U4A29_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEO0681506.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="U4A29_002406"
     CDS             872..2512
                     /locus_tag="U4A29_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEO0681507.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3982
                     /locus_tag="U4A29_002407"
     CDS             2666..3982
                     /locus_tag="U4A29_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO0681508.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAVPLSTIYANVLGTDASSIISRLIPATAMT
                     NVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSLTF
                     FILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAVLA
                     GIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLCNN
                     SMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4050..4484)
                     /locus_tag="U4A29_002408"
     CDS             complement(4050..4484)
                     /locus_tag="U4A29_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0681509.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4549..5385)
                     /locus_tag="U4A29_002409"
     CDS             complement(4549..5385)
                     /locus_tag="U4A29_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO0681510.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1614 a         1083 c          989 g         1726 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccgttccttt
     3241 atcaacgatt tatgcaaatg ttttaggtac ggatgcttct tctatcattt cccgcctgat
     3301 tcctgcgaca gcaatgacaa atgtcttagc tatcatcggt gcagcattag ttgctcgtgc
     3361 tggacaatca atgccaaaat tcaacggcaa tggtaaatta atggaaaaag gcgatcttgg
     3421 cgatggaaaa cctcgaactg tgaaaccttc cattccacag ttaggtgttg gtttgatggt
     3481 atcattgact ttcttcatct taggacaaat tggtaactat tttgttccta aagtccatgc
     3541 ttacgcattt atgattatta tcgttgtcat ctgtaaaatc gccaacctat taccagagta
     3601 ttatgaagat gcagctatca tgttcaataa cttgattgtc aaaaacttga cagcagctgt
     3661 tcttgccggt atcggtattg cgttgctgaa tctgaatgta ttagcttctg ctttgacatg
     3721 gcaatttgtt gtcttatgtc tgacaagtgt tatcgcgatt tcagttgttt caggatttgt
     3781 cggtcgtttg tttggtttat atccaattga atcaactatt actgctggct tatgcaacaa
     3841 ctcgatgggc ggaacaggaa atgtagccgt cttatctgca gctaatcgga tggaacttat
     3901 tgcttttgct caaatgggca atcgacttgg tggtgccatc gttttgatca tctctggttt
     3961 cttgatgcaa ttactttctt agtcaaaaaa acaaaaatac ctgatcatca agtatcggct
     4021 tggtgatcgg gtattttttt gataaagatt tacaggattt tcaaaaatct tttttgcatt
     4081 ttatcggaat gataaccgat gtgttcataa aattggtgtg cttctactct gtattctgca
     4141 gaattaagcc ttattgtcgt tattcctcgt tttttcgctt cttcttctac gccagtcatc
     4201 aaagcctttc cgatgcctct tttttcagca tatttagaca cagcaagtgc cataatattg
     4261 aacattggat cgctaaaaat cgattcatag gactccgctt gtacatagcc agctgctttt
     4321 ttagaagtag gatcttcata aaccaagaaa aaatttttct ctggctcctt cagcagtttc
     4381 ttcatttgtc tcaccgtaac ggttaatggt atatcatacc ctaattgatc cgcattgatt
     4441 tcacgtagtg cttctgcatc ttctaaacgc atttcccgta acatccaaat caccagccaa
     4501 tttctgattt tttaaaatga atttttttat catagtatac tctttttttc atttagaaca
     4561 atatgtttca gtgattttga cttgcgttag tctagattct cctaatgttt gtaagatgac
     4621 aaaggcattg taacaattga ccaaaagaac cacgaggaca attacttgaa aaataaaggt
     4681 cacttttttt ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc caataacagc
     4741 agcggggata acataaaaca acatagataa atcaaagcgc ataaagctac ttgttaaagc
     4801 aatcgttacc aattttgcca gttgagaaaa gaaaatcgta gatagagaat atagtgttgc
     4861 ttcttttatt ggcaatgcaa acatcagcat caacaaagaa acattgactg gtccaccacc
     4921 aatacctaaa aaactagcta aaaagcccag aaccccccca catataaggt accaagtcca
     4981 tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt
     5041 caataccgtc aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa ataactgtaa
     5101 aaaaacttcg aatacaatgt tgccgactac tcctccaaca acagctccac tagataccca
     5161 accgacaatc tgccaattca gtgatttccc gcttgatact tgtcgcacag tcgaaacaat
     5221 ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc
     5281 gataagatca agaatcggtt tgatcaacac gcctccaccc atcccggaaa tagcgccaac
     5341 tgtgttcgcc agaacgatca ccacaaaata aatcagacca atcatcttgt atttcctttc
     5401 tcttgtttat at
//
LOCUS       DASOUD010000129         5286 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.132, whole genome shotgun sequence.
ACCESSION   DASOUD010000129 DASOUD010000000
VERSION     DASOUD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.132"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(266..466)
                     /locus_tag="U4A29_002410"
     CDS             complement(266..466)
                     /locus_tag="U4A29_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO0681511.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="U4A29_002411"
     CDS             complement(771..2003)
                     /locus_tag="U4A29_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO0681512.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2257..2826
                     /locus_tag="U4A29_002412"
     CDS             2257..2826
                     /locus_tag="U4A29_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO0681513.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3004..3444)
                     /locus_tag="U4A29_002413"
     CDS             complement(3004..3444)
                     /locus_tag="U4A29_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO0681514.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3602..4366
                     /gene="map"
                     /locus_tag="U4A29_002414"
     CDS             3602..4366
                     /gene="map"
                     /locus_tag="U4A29_002414"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEO0681515.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4398..>5286
                     /locus_tag="U4A29_002415"
     CDS             4398..>5286
                     /locus_tag="U4A29_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO0681516.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFN"
BASE COUNT         1627 a          969 c         1031 g         1659 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccgtttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt
     2101 tattttttta aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag
     2161 aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt
     2221 ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact
     2281 agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt
     2341 actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt
     2401 aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat
     2461 gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc
     2521 cattttgcaa gaagagagaa gaagttgtat cgttcactgt acttagaaga atatggtgga
     2581 ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca
     2641 gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata
     2701 gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt
     2761 gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc
     2821 gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag
     2881 gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa
     2941 tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt
     3001 atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc
     3061 ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa
     3121 agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc
     3181 gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac
     3241 gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc
     3301 ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag
     3361 attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc
     3421 atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat
     3481 gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca
     3541 tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa
     3601 tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt
     3661 agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat
     3721 tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt
     3781 tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc
     3841 tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa
     3901 aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga
     3961 ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg
     4021 gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc
     4081 gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt
     4141 tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat
     4201 cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc
     4261 gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga
     4321 tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat
     4381 aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc
     4441 gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg
     4501 tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt
     4561 taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg
     4621 agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa agttcaggag
     4681 gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt
     4741 tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc
     4801 gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg
     4861 ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga
     4921 actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca
     4981 tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc
     5041 cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg
     5101 ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca
     5161 tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg
     5221 ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg agatccaagc cgaaaatggt
     5281 tcaata
//
LOCUS       DASOUD010000130         5234 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.133, whole genome shotgun sequence.
ACCESSION   DASOUD010000130 DASOUD010000000
VERSION     DASOUD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.133"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1142)
                     /gene="nagA"
                     /locus_tag="U4A29_002416"
     CDS             complement(<1..1142)
                     /gene="nagA"
                     /locus_tag="U4A29_002416"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO0681517.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERF"
     gene            complement(1218..2054)
                     /locus_tag="U4A29_002417"
     CDS             complement(1218..2054)
                     /locus_tag="U4A29_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0681518.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2238..3455)
                     /locus_tag="U4A29_002418"
     CDS             complement(2238..3455)
                     /locus_tag="U4A29_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO0681519.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3604..3783)
                     /locus_tag="U4A29_002419"
     CDS             complement(3604..3783)
                     /locus_tag="U4A29_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681520.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3838..3921
                     /locus_tag="U4A29_002420"
     tRNA            3838..3921
                     /locus_tag="U4A29_002420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3872..3874,aa:Leu,seq:gag)
     gene            complement(4161..5159)
                     /locus_tag="U4A29_002421"
     CDS             complement(4161..5159)
                     /locus_tag="U4A29_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681521.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1620 a         1072 c          830 g         1712 t
ORIGIN      
        1 tggaaacgtt ccacgccatc tagataagtt gcttccaatg tcatatcagg gttcaatacg
       61 ataaagtctg catcgcgtcc ttgagcgatt tttccacatg tatcatcgat tttacagctg
      121 attgctggaa tcagactagc catcatgact gcttgttctg gtgttgctaa tccccagtct
      181 acaacatttt taattgcttc tttcaatttc aatatacttc cagctaagtt gccagattct
      241 agacgtgctg ttccttcttt tactactact ggaaattcgc ctaaattgta atttccatca
      301 ggcatcccac cggccatcat acagtctgta attaatgcga catgatcatg tcctgctttt
      361 tccattagga ttcccgcagc atttggatgt acatgatggc catcacaaat caattcagaa
      421 aagacatgtt ctaaagataa taacgcaccc accatacctg gttcgcgatg attcaatcca
      481 cgcataccat tgaaagcatg gacaaataca cttgcgcctg cttcaacagc tttttgcgct
      541 tcttctagtg tagcattact gtgtcctaga gaaacaacta ctccttcttc agtcacttgt
      601 ttgacaaact cttctacacc ttgacgttcc ggtgctaaag caattttttt gattaaacca
      661 cctgaagctt cttgccattc atggaaagta tccaaatcag gatccccgaa ataacttggg
      721 ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt caaaatagat tcctttgatt
      781 ttagctcctg gtgcttcctg atacattttg ccgattgtct cagctacatc ttttaaacgt
      841 tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca ctccacaaga taacagacct
      901 tcagacatca ctttgatgcc ttctgcatca ttatccatta catcatgatt catatagccg
      961 tgaatatgtg tatctaccaa accaggagca atccatttgc cgctttggtc aatgatgttg
     1021 acttctccct caggtagttc tttggtgtat tcaccaaaaa gtccatcggt aatttcaaga
     1081 tagcctgcat gtttgacacc tgacgctaaa aagaatttgt cagcatagat aaaagttctc
     1141 attctattct ctccctttgt ttttctacct ctaaagtact cttatttaga gaagaagtca
     1201 agaatggtct acaccattta caaatttttt gaaatttgtt tcacagcttg tgctaagcct
     1261 tcatagaatg ctgctctttc tggttcttct ttacgcttgt atattaagct tttaagcaca
     1321 aataaatcat tgaggtaata gtagctattc ttctcttgag cccaatagat tccttgattc
     1381 acctgttcta acgcttcgtc gatttcattt tgatggaata agaaatttcc ataattataa
     1441 aaaatttgcg aaaattcact ttttgatcgt tcatcagctg attgatgcag cagttggatg
     1501 cttcgactca aatagtagcg tgcttcagcc gtttttcctg ccacaccata tatttttccg
     1561 atacaactaa tcagttgtat ttctgtgtca gaaacatgga ttctttcttt ttggctagag
     1621 tagcttaatg cttctttcaa ataataaagg gactgagaga tattttgacg caacgtgaat
     1681 tcgcaaacac cgagataata ataatagcgt tgaaagtcag ttgcttgata cagattttca
     1741 gaaatatcag aagattttag tacttgttct aacttccgat aatcacgttt ttggaaacaa
     1801 ctatccaaca gttcaaataa tttatcgttt gtccgagtag tttttcgaga gagattcatc
     1861 acatgatcaa tcgttacccc tagccgttga caaatctgca tcatcaccaa tacattcggc
     1921 agttcctcat tattttcgat ccgacttaat acactttgtg aacaaatatc atgcgaaagc
     1981 attttttgcg tcattttttg ttcttttcga atctctttaa tgatcgttcc aaaggactcg
     2041 attaacgttt ccataagtat cctccccaaa atatgcgtat tcgcattcta ttcgtatttt
     2101 actttaggaa gtcgcaaact tcaagaaaag acttgcaact aaataatata ctttagttat
     2161 ttaagaaaac tccactagaa agagagcctc agtgtgtttg tctgtggccc tcctactgaa
     2221 aaatttggtt tttaaaatca tttttggcga taggttgcta ggaattctct cattttctta
     2281 gctgtttgtt tcaaatcatc cattttagga agataaacga ttcgaaaatg atctggctgg
     2341 ttccaattaa atccaccacc atgtacaagt agaatatgat gttcatgtaa aaagtccaaa
     2401 acgaattttt catcatccaa aatattgaat cgctttgtat caatttttgg gaatatataa
     2461 aaagcagctt ttggttttac ggctgaaaga ccaggaatat cattgattgc attatagata
     2521 tattctcttt gttcatagat ccttccgcct ggcaggagca gctcatcgac actttgataa
     2581 ccgcctagcg cggtttggat aatttgctga gacaatacat tggaacacaa acgcatggag
     2641 gacaacatat tcagcccttc tatatagcct ttgacgtgtg acttatctcc acttaggacc
     2701 atccagccta ctcgaaatcc tgctacacga tgagatttgg atagaccgtt cagcgtaaca
     2761 acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt ctagaccatc catcactaag
     2821 cgatcataga tttcgtcgga ataaataatc aggttatttt gtcgagctac ttcaacgatt
     2881 tcttctaata attcttttgg ataaagtgcc ccagtaggat tattaggatt aatgataaca
     2941 atagcttttg tccgactcgt tatttttgat ttgatatcat caatatcagg ataccattct
     3001 gcctcttcat cgcaaatata atgaacaggt gtgcctccag ctaatgcaat agaagccgtc
     3061 cacaacggat agtccggcat tggaaccagt acttcatctc cattgtccag caacccctgc
     3121 atacacatcg tgatcaattc gctcactccg ttgccggtgt agatatcatt gatcgtcaca
     3181 ttcggaaaat gtttcagttg gcaatattgc tcaattgctt ttctagcaga aaagatcccc
     3241 ttggaatctg agtagccttc tgagtcacgg gcattcatga tcaaatcacg aatgacttcg
     3301 tttggtgctt caaatccaaa aggtgcggga tttcccgtgt tcaattttaa aatactaatg
     3361 ccctcttctt gcatccgctc cgcttcttct agaacgggac cgcgaacatc gtaactgaca
     3421 ccttctaatt tgttggattt ttcaaattct ctcatcactt agccaatccc tcatttcaaa
     3481 aagttagaac tttccaaatt ttttgttatt tactatttca atatcatact ccatcctaga
     3541 aaattttgcc acttatttag gaaaaacgta agtattaaaa aaacacaaac aagcgttgag
     3601 aacttaattg tcatgatagt cattccatag attcgctgcc caatccattg caattccaca
     3661 aatgattcct agcataaaac caagcaaaaa acgtccgaat gcaagtccaa tcaatacacc
     3721 taagatcatt cccagtcctg aaaattctcc gtcatatttc tccatcttaa taagtcctcc
     3781 catttttgag cttgacaact atgtaaaacc tcgctatcat tagtgtgtta ttattacgca
     3841 gatgtgttgg aattggcaga caagcatgat tgagggtcat gtgagcgtta gctcgtgtgg
     3901 gttcgagtcc catcatctgc actacaactc gtgaagttaa ttttgcttca cgagtttttt
     3961 tgtttgaacg tttctttatg ccattatata catatcactg tatcctttac aaaaagaacg
     4021 tacaatcatg taaacagcct gaggtgagta tatgaaggtc ggaaacaagc tactcgtaga
     4081 aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa aatccctcac ctaatactaa
     4141 agcgagggat tttttgtata ttatctgttt atcaccgtta ttataaaggt acaaatacca
     4201 agcacggtta atccaaattg cataaattca tttttttgta cactcatgct tttcattagc
     4261 tctttaactg ccattagtac taagatggcg ataacaacat aaagtatata gtacgtccgc
     4321 attccggaag cccatactgt gggagaaaat cccataatta accttgaaca aaatcccatt
     4381 atcatcaaaa taactaaaaa aatagaagtt ttcttattgt caaaacttaa ataaattcca
     4441 acaattaaac atagtaataa tgccaagact actaaagtag ctatccaagt acctggataa
     4501 aaaagtgaca gtttcgttcc taactctgta aagagattat tcaggttact actactccaa
     4561 atcattgccc ttttatttcc attgacgtaa gtaaagcttt gacccattgt attaccaaaa
     4621 taaataatca ggtttaagaa aaacggtaat gcagtcaata tacgcacata atagttttga
     4681 catttcctat atgttagaag gaaaattaag aaaaatagca ataaaaataa gatattcatg
     4741 tctaaaaaaa acggctttcc aaatgatgaa atacctaaat ctaacttatt aagaaagttg
     4801 aaatttttat attcaggaaa ccatttatta atttcatgtg cagcacgtaa tgcattccct
     4861 ggagttgtca aagagaaaat taattctgct aagcttatta ttgaaaatac tgataactta
     4921 taattatatc gtccattgac aatcaaatat aaactaacta tacttgttag aacaaaaaaa
     4981 caggcgttga cttgttcttg attagttaaa aatatcaata aaggaatact tacccaatat
     5041 atttttctat ttatttcttc tcctcttaag agttgaaaaa acggataaaa agttagtaaa
     5101 ctaaatgcta ctggccactg atagttcaag gtggttgctc tccaaccggt ttcccccatt
     5161 actgtcaatg gtattaaaca gtatatagag caaaccaata ataaattttt aacatttatt
     5221 ttctcaaaaa cata
//
LOCUS       DASOUD010000131         5222 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.134, whole genome shotgun sequence.
ACCESSION   DASOUD010000131 DASOUD010000000
VERSION     DASOUD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.134"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(182..1144)
                     /locus_tag="U4A29_002422"
     CDS             complement(182..1144)
                     /locus_tag="U4A29_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO0681522.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="U4A29_002423"
     CDS             complement(1146..2585)
                     /locus_tag="U4A29_002423"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO0681523.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="U4A29_002424"
     CDS             2770..4716
                     /locus_tag="U4A29_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO0681524.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4980..>5222
                     /locus_tag="U4A29_002425"
     CDS             4980..>5222
                     /locus_tag="U4A29_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO0681525.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1687 a          881 c          865 g         1789 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattgat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga attttaaaaa atgataaaaa
     4861 ttcaaaaaga aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta
     4921 aatatgatat atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta
     4981 tgctatacgg tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     5041 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt
     5101 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     5161 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     5221 aa
//
LOCUS       DASOUD010000132         5190 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.135, whole genome shotgun sequence.
ACCESSION   DASOUD010000132 DASOUD010000000
VERSION     DASOUD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.135"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(297..1337)
                     /locus_tag="U4A29_002426"
     CDS             complement(297..1337)
                     /locus_tag="U4A29_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO0681526.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            2503..2589
                     /locus_tag="U4A29_002427"
                     /pseudo
     CDS             2503..2589
                     /locus_tag="U4A29_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            3149..3367
                     /locus_tag="U4A29_002428"
                     /pseudo
     CDS             3149..3367
                     /locus_tag="U4A29_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3627..3881
                     /locus_tag="U4A29_002429"
     CDS             3627..3881
                     /locus_tag="U4A29_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEO0681527.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            3882..4199
                     /locus_tag="U4A29_002430"
     CDS             3882..4199
                     /locus_tag="U4A29_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO0681528.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            4758..>5190
                     /locus_tag="U4A29_002431"
     CDS             4758..>5190
                     /locus_tag="U4A29_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681529.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT         1818 a          736 c          794 g         1842 t
ORIGIN      
        1 atatcaaggt ttttagcttt tttaaaaaat gaaattacct ctcttcgcgt attattataa
       61 ctaaaaaaca gcaacatgca aacgtttgtt ctcttttttg tgttggaaat taattattaa
      121 ctattaacac ttatgacgta aaaagctacg tttgtgattt ttatcataat attttaactc
      181 ataaattcta ttaaaatagc tatttctttg taacatttgt aatataatta gaataataaa
      241 aaatttttaa ttttaatgct actccatgaa gcaacaatca atttgtcagt gaaatttcac
      301 tctaaccagt ctttcgtaat agtttttggg aagctttcag aattcaaacg tttttcactt
      361 gatactttag cagctattgg tttttcgata aaccaatttt ttacatagac aaaaatcagg
      421 ttctggatat tttcaacttt ttcggttttt aatttttgat aggcttttaa cagtgtcgtg
      481 tataattctt gttctgcatt gacataataa ctatcgagtt cttcaaaaat tattgaaata
      541 ccactttctg tttcggcttt tttctttgca ttatgaatag tctggacagt tttttgagct
      601 tcactgtaac ttgaagaaaa tgtttttatt aaatttaata ctcgttcagg gataaacgta
      661 gcaatgctgt cgtttgtcat aattgtctca aaattgtcta acaacaattg atcttgtagg
      721 cggtcttttt cagtgtctat attgtgtcta ttagtgtcta atatattatt attaagttct
      781 ggtatgattt tcataccctc tgatttatca acgttttggc acggtatgat tttcataccc
      841 tcttgagccc caagggattc agcgtctgat ttttcgtcga gggtgggtat gattttcata
      901 ccctctgatt tatcaacgtt ttccctattc tctctatcaa atttttcgag taagtagata
      961 tcattttctg agacttttaa ttctgctaga tatagtctat taggattatt tttccctaac
     1021 tgtttgttaa atcccatctg cttttgtaat aataaaccaa aggattccaa ctcttttttg
     1081 attgctatcg ctttttgttt ttggcagttt aatacgctac atagttcatt tgtcgtataa
     1141 gtaaaataaa cattaccttc ttcatcgatt tgccgtttgc tgattgcgat ctctaatctt
     1201 gcttttaaaa gcatataggc aatcttagtt gaatctttca tttttttgta ttcgtcagaa
     1261 acaataaaga cttttggaaa ttgataaaat aattcgttgt aaactctatc cgccttaaaa
     1321 aaattaaaat cagtcattgt agatcctcta ctttcacgtt gttttattta tgtaaaatta
     1381 cctactcttt tcgaaaatag caaagataaa taaaaaatgc aaacgcatta aaactgtttt
     1441 ttaattaaat actacctaaa atcagaaata aaatgaatta tgtactacct ttaaagtcat
     1501 taagaactac tttaaataaa tattatttat aattaaatac taccagaaga acgtatgaat
     1561 aaaatttagt tttaaaatta aagataatta agtactacca gtgtcaaaac aaaaaataaa
     1621 ttaagaacta ccttatgcat ataaaacata ctaccattac tagtaatata tactacctta
     1681 tttcagtagt atatattata ttcttcttat aagacaaggc tttaggctag aaaacaatag
     1741 attatatact actaaaaaat acaataagta ctaccaatgt cagatatata taataattat
     1801 gtactaccat taaaattaac ttatttaata agatactact gtgttaatct gaaaaaaatt
     1861 actttaaagt agttacatac tacctgttaa attagatact accattaata cttaaattag
     1921 atactaccta tatttatatt aagtactacc gttctaggaa taagataata aaaaaaaact
     1981 accaaattgc aattaaatac tacttaaaat gtaaaaaata agaactaccc aaattaggta
     2041 gttcttaata agagaatatc agataaattt gtacaagtca taaaaaaatt aatcttaaat
     2101 ttttaaatat atatacctgt taattataaa ctactttatt taggtagtac ataattaaca
     2161 gataaaacta catgcttgta gtttgtctag ctaatgagtt gcatatgatt tatataaagg
     2221 tgtgcagcat aacttagttg aagttaagag aatagaataa atattttttg ttttgctaat
     2281 aattaaacac tacctaaata tggtagtgtt taattatata atattcattt tgttacaaat
     2341 aatcgtatta taaaagcata acaattggga taactatttt ttagattatt taagtactac
     2401 ttaaattagg tagtacttaa attttggttt aaggtagtag gtaattaaga aataatatat
     2461 atcttctttt ataatagaag gggagaggcc tagttaaaaa cagcgacaat taataacaat
     2521 gtatttaatg cttcacaaaa tccatttggt ggacaatgca ttgttattag cgatgatcta
     2581 ccgttctaag tagttttttc tattggttgc attcgtgact aattatggta taattatgag
     2641 taacaaagag gtatttcatt atatcatcaa accccatcag cgtgtaggct gatggggttt
     2701 tttttacgtt tctatattat agaattaact ggtaggctta ctgtcttttt tggcaatttt
     2761 gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct tgattgcaaa
     2821 aacaaaatca aaaaggtatt tcattatatc tcctcctccc cttgccagat taggccgggg
     2881 tagcagacag caaaactatt ataacataac tgaaatacct aaatgacata tacagatatg
     2941 ataatttatg tattgatttt tctaacaaaa gcacatattt taatatatac tacctatttt
     3001 aggtagtata tatttttaga tacaatatcg atcagttttg aggaaatgaa attctagata
     3061 ttttttgcta catagagtta ctttcaatat tttttaacgt aattaggcag aagtcactca
     3121 tcctctatag gtttgtgatg aaattgctat tgatccattt aagtaagagc aaaaaactgg
     3181 aggtttatca ggtaattggt ctaatggatt taagtatgcg gtcaccgtgt atagagttgc
     3241 ttatgagatt caagaaaata aagttattcc agtattgcta tatggaacac ataacaattt
     3301 ttatgaatta gccgagaaca ctcgcaactt tattcgtgta gatgaatcgg cttcggttga
     3361 gttataactt tagctatatc aatcaaccga ccgacatatg ttaaatctat atataataaa
     3421 aatacctact taaatggatg ttaacggaag tgtatggtga gtagttcact cgtattttga
     3481 tatcgtttgt atatgttttc agcgttctaa gtggcttatt ttgtccaata ataatatgga
     3541 gtggaatccc ttaagaaaca ccgttggcaa ttgtcgttat aacatgttat aatttattta
     3601 tcaaaaacta aaagaagtgt gataacatgg gaaaagaact aaaggttaga aagatcggca
     3661 atagcgtggg agtgatcctt ccaagctctc ttggattaaa aagcggagac actatccaag
     3721 ccaaacaaga aggcaactta ttcatattag atactactca aatagcaaaa gaacacgatc
     3781 gcaaattgat tgaagaaagt ttccaagact ttgagaaagg tttaacagtt tcagaaattg
     3841 aaatggtaaa agctttcgga aaatacggct ggagtgaata gatgaaagag tacacgctag
     3901 tttattctaa aagtttccag accagccttg aaacgataat agaagaatgg gaaaatgagc
     3961 ttgctctttc tccagccagc ataaaaagat ttgtaggtgc gatccagaag gctttagaac
     4021 taaccaaaaa ttttccaact atgcatgagg aagtttccga aatctatgga atggatatcc
     4081 caacatatcg gatcgtgatc ggcaaaagct atgccttatt ttatcggatc gaccatgaaa
     4141 aaaaagagat acttgtagga cgtattttcc aatctaaaca aatgaaacta ggcttttaac
     4201 aataattagg caaaagtcgc tcatcctcta caggtgggtg attaatgccg ctcgatatgg
     4261 ttgttaaccg tcggttactc gaagattgat atataagact tagtaccttt ttattttgct
     4321 ccatgttatg gccgaaggtc taaagataca atcattagtc tagagaaaaa ttaagaaccg
     4381 taggaactac ggggatcgct tggtgaataa gagacactgc tattagtgaa aaaataagat
     4441 atcaagtacg ctctattccc aaaaatttcg tgactttact catgagaggt tcaagtagta
     4501 tatttttaat caaaattgac gtaactttta ataaatacta cctaaataag gtagtattta
     4561 tttttgtgtt ttaaaagagg aagtccaata tatctatggg aaacttctta aatattatgt
     4621 tggtacatga aaatatacat ccctgctaga ctatttacaa cattttactt tgtacgtatt
     4681 cttgttttaa aaggaatggg ataaaatata ggtacaataa ctatttgatg ctaaatataa
     4741 ttaatggagg agagatagtg aacatactta atgaaaaaag atatgtatgg tttgctggta
     4801 aaaaatatat tactactttc aaaagattat ccgagttaat gcagcagact gttaaaagtg
     4861 ctatgcctga aggatacaag tttaattttg ttcaatcttc agtatctact gaaagtatct
     4921 atttttcaat tctttctgag agtaggcttt ttcattttag aataagttct catgtaaaga
     4981 aaactgatgt tgatatatat acgttccgta caaataatta taaaaattta aatgatttga
     5041 gtagaagtat cagtgaatat ctcaagggga atagctatct caagattcga ttttcacata
     5101 tattaatttt actaccatat ttgaatgaga atttcaccct aattcaagta gataagtctt
     5161 tcattgcctc aggtaatcaa ggaaatatga
//
LOCUS       DASOUD010000133         5067 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.136, whole genome shotgun sequence.
ACCESSION   DASOUD010000133 DASOUD010000000
VERSION     DASOUD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.136"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..832)
                     /locus_tag="U4A29_002432"
     CDS             complement(<1..832)
                     /locus_tag="U4A29_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0681530.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1643)
                     /locus_tag="U4A29_002433"
     CDS             complement(846..1643)
                     /locus_tag="U4A29_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO0681531.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1676..1960)
                     /locus_tag="U4A29_002434"
     CDS             complement(1676..1960)
                     /locus_tag="U4A29_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEO0681532.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1957..2958)
                     /locus_tag="U4A29_002435"
     CDS             complement(1957..2958)
                     /locus_tag="U4A29_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEO0681533.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2960..4027)
                     /gene="gnd"
                     /locus_tag="U4A29_002436"
     CDS             complement(2960..4027)
                     /gene="gnd"
                     /locus_tag="U4A29_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEO0681534.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            4026..4874
                     /locus_tag="U4A29_002437"
     CDS             4026..4874
                     /locus_tag="U4A29_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO0681535.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         1522 a         1096 c          835 g         1614 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgccg catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat ctgttataaa aaaagcagct
     1501 tgtctgtaaa agtgttctgt tttcccttct gcttgtccat tctccttata catgacagca
     1561 agaggtgtcc atggagcaca attactagct aaagtaggga tgactccgaa atcgattctt
     1621 gcaagattgg cactataacc cactagatcg gtcagttttc ctccgccaac gccaactacg
     1681 aaatcgattt catttttttg atgattgtag ctattcgtct ggcttcagca taactacatt
     1741 ctccagaata acgatgataa aaaaattgaa tcgtttcgtc attcaaaaat actaatttag
     1801 gtagcgcctt ttcccatgat accgttccgt gggtgaccag tactcttttc gcgccgtatg
     1861 atttcaagag atcagaaact tttgaaatac caccttcttc atataaataa aattgagggc
     1921 ctgttttcac ttttaaaccg ttcaccattt tcttcatcat ccttttatat agttgctctt
     1981 tgtctggaca gaacgttcag gaagaaggtg ctgactgatc tgttcgacat ctctgacaca
     2041 tttttcaccc attgcttcta aacattccct tgtgtaggcc gatatatgtg gcgtaatcac
     2101 tacttgttca aacgctaaat acggatgatc ttgacgtccc ggttcttctt ctaaaacatc
     2161 tgtggcatat cctgcgatct ttccgctttt cagaccatta atcatcgctt cttcatcaac
     2221 tagagcgcct cttgcactat tagatagata aacattttct ttcatctgat cgacttcttt
     2281 atcagaaatc atgtgatagt tctcttctgt caaattcgca cataaacaaa tgatatctga
     2341 ttgttccagt aattcttgaa gttctacttt ttttgcacca aagctttcga tgtataaatc
     2401 tgatttgtaa ggatcataag ctagaacatc acagcgaaaa ccgttacgca aaatttcgat
     2461 tactccgctt ccggtatttc ctgcaccaat cacaccaact gttttgttaa acagcgtcct
     2521 accgacgaac tccgcccgat cttcccatcg atcgtccttg actcttgctt gtgcttgtgt
     2581 cgtgcgtcga agcatggaca acaaattagt tatattattt tctgctacag catttcgttc
     2641 aactaaagca ggtatgatag atacgattgt ctggtgttct tttgctgctt ttaaatcaat
     2701 attgttgtaa ccaataccat gtcgggaaat caatagcagt tcatctttgt tttcaaaaaa
     2761 atctcgtcga aaaaatggcg taacacttgc aataatgata ttgaatcctt taagtaactc
     2821 cgctagttct tttcctggaa tctcttgatc aacagtaaag aaatgaacgt ctccaactga
     2881 ctttaatctt tccaagtggt gtggaaagac tcttccgaaa ctgctggaat taacgattgc
     2941 tatatgataa tctggcattt tactcaccct tttcaactat ttcatgtccg ccaaacccat
     3001 ttcttagtgc tgaaactact tttgctgaaa aactatcttc ttcctgtgaa agattcctca
     3061 tgaacaaact agttgcaatt actggaactg gaacagccat ttctagcgct gtttcaaccg
     3121 tccattttgc ttcacctgat gcagaaacga ttccccgata attacttaaa ttgggatgtt
     3181 cgtttaactg atcttccatg atttccatca gccagccgcg aatcaccgaa ccatgattcc
     3241 aaacacgtgc gacttgttcc aagtcaaaag catactcact tttttgtaca agttggaatc
     3301 cttcaccgat tgcttgcatc attccatatt cgattccatt atggatcatt ttcaagaagt
     3361 gtccgcttcc ggatgcacca gcatacagat atcctctttc aattgctaca tctttgaata
     3421 attgttccac ggatagaaaa gtttctttat ctcctccaat cattaagcag gcttgatgtc
     3481 gtgctccttc gattcctcca gaagtcccac aatccaagta accgatttca tttttctgaa
     3541 actcttctgc tcgacggatt gaatctttat aataagagtt tccgccttca ataataatgt
     3601 ctcctggtcc gagttttgga aaaagttttc ttaacatgtt ttcggttatc tctccagcag
     3661 gaagcattaa ccagatgatt tttttctgct caaaagtagc tagaaattca gagatctctt
     3721 gataagcagg aatgtcattt tctcttgcct gactaacagc tgtacttgaa atgtcgagtc
     3781 cttgtacttt gtgatggtgc tcttttaagt ttaaagctaa attcaatccc atttttccca
     3841 aaccaattac accaatttcc atgatagcgc ctccatttct tataccctca ttatacaaaa
     3901 aaattttttt gtaaatagat tttacaaaaa tattttttgt ttgatttttt cctactctcc
     3961 ttgtatcttt aacattaatg agaacggaaa aaatgataat cttattatga aaatgaggaa
     4021 ttctcatgag cgtctccata agactacaaa tcaaaacaat gtacaacgac ttcagcccaa
     4081 aagaacaggc tatcgccgat tatattttag aaaatccaag caaagtaagt catagtccta
     4141 ttagcaattt agcaagtgaa ctgggcattg ctgactctac tttttttcaa tttacaaaaa
     4201 aacttggtta taatggattt aaagatttta aaatggccat gttgatccaa gaaaatgatc
     4261 tatctgctat ttctatccac gaaaatattc agaaaacaga tactgagctc actatggcgc
     4321 aaaaggtgtt cgagtctagt atttcaactt taaatgacac aaaaaagttg ttacaacaaa
     4381 aggatctaaa aaaagcggct actattatca atgaatctaa acgtgtctat tttttcggtg
     4441 ttggcggttc agagattgta gcaactgacg cttatcataa atttttgcgt tcccctatcg
     4501 caacttctca cagcacggat taccatattc aattgatgga agcttcttta ttgacggaaa
     4561 aagactgtgc tgttttgatt tctcacacag ggcaatcaaa agaaacaatc catattgctg
     4621 aaaccgtaag aaaaactgga gcaaaaacaa tcgttattac tagccaagct aattcttccc
     4681 ttgctaaact tggcgatgtc gtttttattt ctatttctga agaaacagaa ttccgatctg
     4741 aagctttagc ttcccgtatt tctcaactaa gcattttgga ctctctatat gtgattttga
     4801 tgttttacaa cgaaaaagaa tcacgtcgtt ctatcgctaa agttcgacat actatttctg
     4861 aaatcaaaga atagcaccat aacagaaaaa gcgaaaaatc cataaaacaa aaaaagccac
     4921 taccaaatat ttggtagtgg cttttttggt tctgcaaggg tggtacagaa catcaatagt
     4981 cggtgggtta gaacgtatgt catcgcatct ttggagaagg ttgtattggt attattcgag
     5041 ttacttgtta gatgcgataa caaagtt
//
LOCUS       DASOUD010000134         5034 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.137, whole genome shotgun sequence.
ACCESSION   DASOUD010000134 DASOUD010000000
VERSION     DASOUD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.137"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="U4A29_002438"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="U4A29_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO0681536.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="U4A29_002439"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="U4A29_002439"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO0681537.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="U4A29_002440"
     CDS             complement(2169..2906)
                     /locus_tag="U4A29_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO0681538.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="U4A29_002441"
     CDS             complement(2997..3524)
                     /locus_tag="U4A29_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681539.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="U4A29_002442"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="U4A29_002442"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEO0681540.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="U4A29_002443"
     CDS             complement(4401..4886)
                     /locus_tag="U4A29_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEO0681541.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1337 a         1125 c          862 g         1710 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acct
//
LOCUS       DASOUD010000135         4963 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.138, whole genome shotgun sequence.
ACCESSION   DASOUD010000135 DASOUD010000000
VERSION     DASOUD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.138"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            115..1002
                     /locus_tag="U4A29_002444"
     CDS             115..1002
                     /locus_tag="U4A29_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO0681542.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1142..2068
                     /gene="cysK"
                     /locus_tag="U4A29_002445"
     CDS             1142..2068
                     /gene="cysK"
                     /locus_tag="U4A29_002445"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO0681543.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELRKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2304..2750
                     /locus_tag="U4A29_002446"
     CDS             2304..2750
                     /locus_tag="U4A29_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEO0681544.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            3023..4375
                     /locus_tag="U4A29_002447"
     CDS             3023..4375
                     /locus_tag="U4A29_002447"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEO0681545.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4546..4833
                     /locus_tag="U4A29_002448"
     CDS             4546..4833
                     /locus_tag="U4A29_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681546.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKSTINIDELADQVLSQLNETQEE
                     YYTMPAKDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1703 a          820 c         1034 g         1406 t
ORIGIN      
        1 aattcttcgg attagtttgt gtttcttgag aattaaaagt ataatttaat taattaagtt
       61 tgttttaaga agagaacttt ttattaagtt tttttatttt gaggaggaat ttgaatgcaa
      121 gagctgaaga cccgcagtgc ccgacaccat agacctaaaa gaaaaatggt gatcaataaa
      181 aaagcgattg ctcctttaat tggaacgagt cttttagttg ggccgatgat ggttacagcg
      241 gtaccgcaac atgttcatgc agatgaacaa ggatctcaaa cagtcgctga tcctcaagcg
      301 tttatcgatc aaatcggctg gtctgcgtca gaagttgctg caagtaatga cttgtatgct
      361 tcagtcatga tcgctcaagc gttgttggaa agtgcatatg gcacttccgg attagctagt
      421 gcgccaaatt ataatctatt cggtgtgaaa ggaagctata acggacaggt cgtttatatg
      481 gcaaccaaag aatatgtaaa cggacaatgg gtaacgatga atgaaccatt ccgaaaatat
      541 tcttcttatt gggaatcctt ccaagaccat gcgaatgttc ttcgttcaac cagtttttcc
      601 acgggcgatt atcattatgc tggcgtgtgg aaaagcaata caacaagttt ttatgatgca
      661 acagcgtact tgacaggtcg ttatgctacc gatccaaatt atggggccaa attgaactca
      721 ttgattaata cttataattt aacccgtttt gatacgccat caacaggttc ggctacaaca
      781 atcacaagta caacaacaac gactactacg acgacttcaa cgtcatcatc ttcaacaaca
      841 gatgtaaata caaataatgg tcagacatat acagtcgtgt caggtgatac gctatgggat
      901 attgcacaaa aatttggcat gtctgttgat gatctaatgg cgaaaaatgg catgtctatg
      961 tccagttatt ctttattagt tggtcaagaa ctaaacgttt gatttagata aaaaggcaaa
     1021 gaaactacca tatatggtat atctttgtct tttttttgct agtcaaagac gaatcactcg
     1081 ccataaatga acagattgta ttatgataag taagaagagc aaagacaagg aggaattgaa
     1141 gatgacagct atctataact cagtcaccga attgattggc aaaacgccaa tcgtcaaatt
     1201 aaataaaatc gtaccagaag attcagcaga tgtttttgta aagcttgagt tttttaatcc
     1261 aggaggaagt gtaaaagatc gtattgcttt aagtatgata gaaaaagcag aacacgatgg
     1321 tctactgaaa ccaggtgata ctatcattga acctacttct ggaaatacag gaatcggatt
     1381 gtcgatggta ggagttgcaa aaggatataa agtaatcatc gtgatgcctg agacgatgag
     1441 tatcgaaaga cgtttattga tgaaagggta cggcgcagag ttgattttga ctcctggtgc
     1501 ggatggtatt tctggatcaa tcagagaagc agaacgtttg gcaaaagaaa acggctactt
     1561 tttaccatta caatttgaaa atgaagcaaa tcctcttgtg catgagaaga cgactggtcc
     1621 tgagatacat caagcatttg gagtaaatgg cttggacgcg tttgttgcag gtatcggtac
     1681 aggaggaacg atcactggcg ctggccgaga attaaaacgt gtgtatccga aaatcgaatt
     1741 aatcggggta gaaccagcag aatctgctat tttagaaggg aaagaagcgg gacctcacaa
     1801 aatccaagga atcggtacag gttttgttcc taaaacactg gatacttctg tgtatgataa
     1861 agtcctttct attagtggag acgaagcgat ggaaacagct agagaagttg ggcgaaaaga
     1921 agggatttta gtaggcatct catctggcgc tgcaattgct gccgcgctaa aagtcgcaaa
     1981 agaattaaga aaaggcaaga aggtattagc tgtggttccc gacaatggtg aacgttattt
     2041 atctacagcc ctttaccaag agatataagg cacttggttt ttctccagat aggtttatgt
     2101 aaaaaacttt taaagatgaa aagaacacac aaaaatttat gatgaaaaaa attatttgaa
     2161 tagagacgta gaatagtgaa cgcgactgtt ttacgtcttt tttcattaaa atacaataaa
     2221 attgaatttt cttttcatgt taagtggatt ttaaaatgta aaaatagtat gataagacga
     2281 gaataacaac gtggggtgtt tacatggaaa ataagttagt cgaagaggtt atcgaaaagt
     2341 taaaaaaaga gaacattcga atcacacctc aacggtacgc ggttttagaa tatttgattg
     2401 aacatcattc ccatccaacg gctgatgaga tttataaagc acttgaacat cggtttccaa
     2461 atatgagtgt tgcgactgtt tacaataacc ttcgcttatt tacttcgatt ggatttgttc
     2521 aagaaatgaa gtacggcgat gcttctagcc gatttgattt cagttcaaag cgtcattatc
     2581 atgtaatttg tgaaaactgc ggtaagattg ttgattttca ttacccaggg cttgaagata
     2641 tagaaatggc tgcgagcaga ctgactgatt ttgagattaa tgaacatcgg ttggaattgt
     2701 atggactctg tccggattgt aaagagtcag caaaaaaggc gcaaatgtaa aaacagtttc
     2761 tacaagtgtt atacctaaaa aaagctagct gtgtaaaaaa aacgctagct tttttatttt
     2821 tttgtctttt attcacaatc ttagatatca aagtgaatat tatcacaata agaatcttgt
     2881 agatattggg ttcttatgct tgaaaaggaa tgaaactaca aacaaacctt tgtgaattca
     2941 caagcattaa actgttgtat ccgttttatt tctgttatag tagttttgtc gaaggacaaa
     3001 atgttaatcg gagggaccat aaatgaaagt agtagtaatt ggctgtacac atgcaggtac
     3061 tgcagctgta aaaagtattt taaaaaatca tccagaagca gaagtgacag tatatgaaag
     3121 aaatgacaat atttctttct tatcttgcgg tatcgcactt tatgtcggcg gagtagtaaa
     3181 ggacccagca ggcttgttct actcaaatcc tgaagaatta tcttcattag gagcaacagt
     3241 taaaatggaa cacgatgtaa cagacgtcga tacagaaaat aaaacagtaa cggcaaaaga
     3301 tctgaaaaca ggagaaacag aaacggtttc ttatgataaa ttagtgatga caactggttc
     3361 atggcctatt atcccaccga ttagtggaat cgatgcgaaa aatgtactcc tatgcaaaaa
     3421 ctacaatcaa gcaaatgaaa tcattgcaca agcgaaagaa gcaaaacgtg tagtaattgt
     3481 tggtggtgga tatatcggta ttgaattagt agaagctttt gtcgaatctg gaaaacaagt
     3541 gacattgatt gatggattag atcgcatctt gaataaatat ctagataaac cattcacaga
     3601 tatcttagaa aaagaattga cagaccgtgg tgttactctt gcgctaggcg aaaatgttac
     3661 tgaatttatc acagatgaag aaggaaaagt gaaacaagta gctacaccaa cagatacatt
     3721 tgatacagat atggtcattt tatgtgtggg cttccgtcca aatacaaaac tgatcgaagg
     3781 aaaagtagat acactgccaa acggggcaat caaagtgaat gaatacatgc agacaagtaa
     3841 tccagacatt tttgcagctg gagactctgc agtagtcaac tacaatccaa gtggcacgca
     3901 aaactatatt cctttggcaa caaatgccgt tcgtcaaggt ttgcttgtgg gaaataactt
     3961 gacagatcat aaaatggcgt atcgtggtac tcaaggaact tcaggacttt acctatttgg
     4021 ctggacgatc ggttcgacag gtgtgaccaa agaaagcgca actttgaacg atctagaagt
     4081 ccaagcgact gtatttgaag ataattatcg tccagagttc atgccaacta ctgaaaaagt
     4141 aatgatggag ctagtctatg aaaaaggaac gaatcgtatc gtaggtgctc agttcatgtc
     4201 caaatacgac attacacaat cagcaaatac catgtcttta gctgttcaaa ataaaatgac
     4261 tgtagaagat ctcgcacttt cagatttctt cttccaacca cattttgacc gcccatggaa
     4321 ttacttgaac ctacttgcac aagcagcgct aggcgaaatc gaaactgcac aataaagaaa
     4381 aagggttgtg acacaatttt gtcacgaccc tctgtctttt tttgtgaaaa gtacaattag
     4441 ttgtctttct tcgaattttt tgtcacaata agcagcaaag ataaagctgt tacataaaag
     4501 aacaagcagc tttatttgat tttgaaaaga aaggtgagaa agaagatgaa taaagaatat
     4561 tttgtccaac aagatgcatt tttagaagat tataaaagtg cttcgaagaa atcaacgatc
     4621 aatattgatg agttggcaga tcaagtactc tctcagttga atgaaacgca agaggaatat
     4681 tacacgatgc cagcaaaaga cgccaaagat aatgtttctc atcgttttcc tttcagtaga
     4741 cgcatctact cagatgaaaa tgacggcaca ttgaatacag agtacacata tgaaggtcag
     4801 ccatatgtgc tagaaactga tgatgatctt taaagacagt ttttgaaaag gccgctcatc
     4861 cttaagtgat gacaaaaaat catcacaatt accgatgact gtctctttac tatcttttaa
     4921 tcagaaaaag gggttatgac aagctttgtc aacccctttt ttt
//
LOCUS       DASOUD010000136         4858 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.139, whole genome shotgun sequence.
ACCESSION   DASOUD010000136 DASOUD010000000
VERSION     DASOUD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.139"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..518
                     /locus_tag="U4A29_002449"
     CDS             <1..518
                     /locus_tag="U4A29_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEO0681547.1"
                     /translation="ETCLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSG
                     INYGEIYSTAACWFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQ
                     RVEDFTKKFSNKLMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSL
                     DVRRYMWQMKY"
     gene            500..961
                     /locus_tag="U4A29_002450"
     CDS             500..961
                     /locus_tag="U4A29_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEO0681548.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1011..1475
                     /locus_tag="U4A29_002451"
     CDS             1011..1475
                     /locus_tag="U4A29_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO0681549.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1489..2238
                     /locus_tag="U4A29_002452"
     CDS             1489..2238
                     /locus_tag="U4A29_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO0681550.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2238..3068
                     /locus_tag="U4A29_002453"
     CDS             2238..3068
                     /locus_tag="U4A29_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEO0681551.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3068..3715
                     /locus_tag="U4A29_002454"
     CDS             3068..3715
                     /locus_tag="U4A29_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO0681552.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(3813..4589)
                     /locus_tag="U4A29_002455"
     CDS             complement(3813..4589)
                     /locus_tag="U4A29_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO0681553.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            4671..4814
                     /locus_tag="U4A29_002456"
                     /pseudo
     CDS             4671..4814
                     /locus_tag="U4A29_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1653 a          664 c          868 g         1673 t
ORIGIN      
        1 gggaaacttg cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca
       61 aatttgaaat aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca
      121 caattggtag cggtattaat tacggagaaa tttactccac agcagcttgc tggttcatgg
      181 aaatgcaatg gattaattct aatgctattc attctgggga atttttccat ggtccttttg
      241 aaatcactga ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt
      301 tagatcaacg ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc
      361 aagctgattt tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac
      421 ttagtggcgt tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct
      481 tagatgttag acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat
      541 ggcaaatcaa ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt
      601 aaaggcaaca aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa
      661 agaaaatact agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca
      721 tttgcaacct ctaattattt taacggattt aattggagga acacctaata atgtttctat
      781 taaacagttg aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact
      841 ggagttatta atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt
      901 actgaataac tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta
      961 aggtggcaat ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat
     1021 tagtgcgtgt agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt
     1081 atgatattaa ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga
     1141 tggctttgac aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg
     1201 ttaaagggat tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta
     1261 aggaagcttt tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg
     1321 tgtctaagaa aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat
     1381 tagaagccac aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat
     1441 cttcaagtat tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa
     1501 gccattttat taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga
     1561 caaaatattg gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg
     1621 catacaggga ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt
     1681 ggggcggctg gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt
     1741 ttaactaata gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt
     1801 gaatttatga gtattatcat gcgcatgata attgctcaat ttggacatgc tgcagataaa
     1861 gccattgaaa ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt
     1921 tttaatgctt ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt
     1981 gtggcagatt tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga
     2041 aatatgcttt ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta
     2101 tatccttact ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc
     2161 gtgacaattt ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga
     2221 gaagaggcgc atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag
     2281 atcttcataa gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac
     2341 aaatgcatat gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg
     2401 atgaagagaa aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc
     2461 aaatgactcc atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag
     2521 aagaaggaaa ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt
     2581 ttgctggaat tggtgattca tttttccaag gtactttaag aattattaca tttgggattg
     2641 gattgtcttt tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg
     2701 ctataccttc tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata
     2761 aataccttac taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta
     2821 tcattggttt ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt
     2881 taaaattcac tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc
     2941 ctggattatt accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta
     3001 gtacaaatat gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta
     3061 tcttataatg attaaggctc taatttttga tatggacgga gttttaattg atagcgagat
     3121 tatgtatcaa aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta
     3181 ttcttatcaa aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa
     3241 tgagggatgt aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa
     3301 gattaattac ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa
     3361 gaaaaaaata aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt
     3421 taccacttgt aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga
     3481 aagtaaacct aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga
     3541 agaatgtctg gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat
     3601 gaaggttctt gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt
     3661 tatttacgat attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag
     3721 ttttttgatg tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa
     3781 attgattgct ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca
     3841 tttttatgca ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta
     3901 tcacaacgaa ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc
     3961 ttttctaaaa taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt
     4021 gttgcagata aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct
     4081 tctagaagtt tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt
     4141 tcttctgatg cgaaaaaatt atcaattccc ataggggcac cgtcttccca cattaaacgt
     4201 ttaatattgt aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca
     4261 gcctttataa ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga
     4321 gaatcgctaa atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag
     4381 gtccctttcc cccttaatcg ttcaactgtt ccactttctg ttaaaagttt aaaagctttt
     4441 cgaatggtaa tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc
     4501 tctcctggaa gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaga
     4561 tataatggtc tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta
     4621 tatcacaatt tattaaaata aaagattaag atataaatat aaatgaacta gtctttgact
     4681 aaccaatgcc cgcttttgaa agaaaacaaa ttcattctgt tttaagtaaa aatccttatg
     4741 ttaccactca ttctgaaggg gatgagcctt atcgctactt ggttgtggaa ccaagtgata
     4801 agacaatttt ataaattcaa tattaattaa aataaaaaca aacaaagtag attatttt
//
LOCUS       DASOUD010000137         4807 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.140, whole genome shotgun sequence.
ACCESSION   DASOUD010000137 DASOUD010000000
VERSION     DASOUD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.140"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="U4A29_002457"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="U4A29_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO0681554.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="U4A29_002458"
     CDS             complement(3250..3768)
                     /locus_tag="U4A29_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO0681555.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          729 g         1713 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DASOUD010000138         4801 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.142, whole genome shotgun sequence.
ACCESSION   DASOUD010000138 DASOUD010000000
VERSION     DASOUD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.142"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..2378
                     /locus_tag="U4A29_002459"
     CDS             9..2378
                     /locus_tag="U4A29_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEO0681556.1"
                     /translation="MVDLKLCNRTVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYG
                     LIEGLQVELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPG
                     GFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRK
                     MQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTY
                     PVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIAD
                     VVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDER
                     NKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTT
                     VIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFA
                     FVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSND
                     TNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPV
                     TEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVS
                     DFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAK
                     RNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVG
                     IISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2458..2853
                     /locus_tag="U4A29_002460"
     CDS             2458..2853
                     /locus_tag="U4A29_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681557.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3178..3795
                     /locus_tag="U4A29_002461"
     CDS             3178..3795
                     /locus_tag="U4A29_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0681558.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3836..4135
                     /locus_tag="U4A29_002462"
     CDS             3836..4135
                     /locus_tag="U4A29_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEO0681559.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4169..4336
                     /locus_tag="U4A29_002463"
     CDS             4169..4336
                     /locus_tag="U4A29_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO0681560.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4381..>4801)
                     /locus_tag="U4A29_002464"
     CDS             complement(4381..>4801)
                     /locus_tag="U4A29_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0681561.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1642 a          667 c          955 g         1537 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgt tttaagtact cagaaatggc
       61 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
      121 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
      181 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
      241 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
      301 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
      361 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
      421 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
      481 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
      541 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
      601 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
      661 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
      721 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
      781 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
      841 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
      901 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
      961 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     1021 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     1081 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     1141 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     1201 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     1261 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     1321 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     1381 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     1441 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     1501 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     1561 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     1621 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     1681 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     1741 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     1801 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     1861 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     1921 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     1981 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     2041 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     2101 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     2161 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     2221 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     2281 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     2341 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     2401 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     2461 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     2521 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     2581 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     2641 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     2701 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     2761 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     2821 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     2881 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     2941 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     3001 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     3061 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     3121 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     3181 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     3241 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     3301 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     3361 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     3421 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     3481 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     3541 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     3601 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     3661 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     3721 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     3781 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     3841 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     3901 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     3961 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     4021 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     4081 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     4141 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     4201 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     4261 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     4321 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     4381 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     4441 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     4501 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     4561 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     4621 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     4681 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     4741 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     4801 t
//
LOCUS       DASOUD010000139         4757 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.143, whole genome shotgun sequence.
ACCESSION   DASOUD010000139 DASOUD010000000
VERSION     DASOUD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.143"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="U4A29_002465"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="U4A29_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEO0681562.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="U4A29_002466"
     CDS             724..1878
                     /locus_tag="U4A29_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEO0681563.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="U4A29_002467"
     CDS             2093..3061
                     /locus_tag="U4A29_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEO0681564.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="U4A29_002468"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="U4A29_002468"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEO0681565.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="U4A29_002469"
     CDS             4091..4699
                     /locus_tag="U4A29_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEO0681566.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DASOUD010000140         4734 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.144, whole genome shotgun sequence.
ACCESSION   DASOUD010000140 DASOUD010000000
VERSION     DASOUD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.144"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..695
                     /locus_tag="U4A29_002470"
     CDS             24..695
                     /locus_tag="U4A29_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO0681567.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="U4A29_002471"
     CDS             754..1194
                     /locus_tag="U4A29_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681568.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="U4A29_002472"
     CDS             1206..1874
                     /locus_tag="U4A29_002472"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEO0681569.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="U4A29_002473"
     CDS             1938..2561
                     /locus_tag="U4A29_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO0681570.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="U4A29_002474"
     CDS             2617..3003
                     /locus_tag="U4A29_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO0681571.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="U4A29_002475"
     CDS             3216..4607
                     /locus_tag="U4A29_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEO0681572.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1576 a          782 c         1007 g         1369 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaat
//
LOCUS       DASOUD010000141         4719 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.145, whole genome shotgun sequence.
ACCESSION   DASOUD010000141 DASOUD010000000
VERSION     DASOUD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.145"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(41..1024)
                     /locus_tag="U4A29_002476"
     CDS             complement(41..1024)
                     /locus_tag="U4A29_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEO0681573.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1029..1676)
                     /locus_tag="U4A29_002477"
     CDS             complement(1029..1676)
                     /locus_tag="U4A29_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO0681574.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1761..2393)
                     /locus_tag="U4A29_002478"
     CDS             complement(1761..2393)
                     /locus_tag="U4A29_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO0681575.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2409..2960)
                     /gene="coaC"
                     /locus_tag="U4A29_002479"
     CDS             complement(2409..2960)
                     /gene="coaC"
                     /locus_tag="U4A29_002479"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO0681576.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2957..3694)
                     /locus_tag="U4A29_002480"
     CDS             complement(2957..3694)
                     /locus_tag="U4A29_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO0681577.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3691..4680)
                     /locus_tag="U4A29_002481"
     CDS             complement(3691..4680)
                     /locus_tag="U4A29_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEO0681578.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         1331 a          983 c          928 g         1477 t
ORIGIN      
        1 aaacgcttct gtcccagcct cttttttgat cagcatgatt ttattcagtt atcttcaaaa
       61 tgatttttcc gatgtttttg tttgcttcca tatattcatg cgcttgtttg actgcgctga
      121 attggaatgt acggtcaatg attggccgaa tcttgccatt ttctagaaaa ggcccagctt
      181 tttgcataaa agtttgtgcg agctctgctt tatactgatc acttctaggt gtcaataatg
      241 taccaagcag ttggacacgt ttcccaatca atacaccaag gtctatctca ggcacgatgg
      301 tcccacctag cataccaatc aagacccaac gtccatcaac ggctatagaa gctaaattct
      361 tttcccagta agaagcgccg atgaaatcca aaataacatc gactccccga ccttcagtga
      421 tttttcgtac ctcttcttga aagtcttgtt ttttatagtt gatcaattca tctgcaccaa
      481 gttctttaca aaaggcgagt ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag
      541 aaagctgtct agctaattgg atcgctgctg ttcccacacc acttgctcct gcatggatca
      601 agacagtttc gccttcttgt aattttccaa gccagtaaag tgtttgataa gcagtcaaaa
      661 aaacttctga gattccagca gcagaaacat agtctagcgt atcagggaga aggattgcac
      721 gatcggcagg catcacagca tattcggcat aaccacctag attgactaat ccgtatactt
      781 ttgtcccagg agtaaaagtg gagatatcac ttttattctc aactacctct ccagctactt
      841 cgactcccaa aatagggtag ggaggtttca aagagactga ttctcttttc ataatatctg
      901 ttcgattgac agctgctgta tggactttga tcagtaattc gccttgtttt ggtttaggaa
      961 caggcacttc aatttgttct aatacatcta cagaaccagg atgcttgatt gaaatggcac
     1021 gcatggatct attcctcctt tgcccattct tggaatgctg tattcaactc tttgctacct
     1081 tcagaaagta ctagagtcgt acgagaaata tccgtttctt ccactagagc ggcaagtaca
     1141 gcagcaactg tagagcgagt gatcgttttt gatgccttct cattaggctt agttaattga
     1201 atggaagtaa cttcttcatc gttagtcaat gtgactggtt gaagaatgac aaaatctaag
     1261 ttagttcgtt tcagccattc atctgcataa tgtttagtaa tataataatc tgtcatggac
     1321 tcaggccatt ttgctggttc atctgcataa acagcactga ccatcagata gcgagagaca
     1381 tgcgcatttt cagctgcgat cactgttttg attgcgccat ccaagtctac ttgcagaaga
     1441 ttctttcctt gagagccggc agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc
     1501 attttttcca cggaccaagt taaatcaaaa gagacatacg aaacattctc tttttttact
     1561 aatgtttggg aagcaacatc tctcacccct gcaaaaattt catgggaact tggagctaaa
     1621 tcttttatca ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat tttcatttgg
     1681 atcatccttt cgacgtcaaa tctttccatt caatagtaac accaaaagag aaaaaaatca
     1741 gagatcggct acgatgacta tcagttagta agagaagggt gcttgaagta tcttaacaag
     1801 acattgcata ctgctgcagt cgcaagacct gcgacgattc cttcaggtat accacttgta
     1861 aaaataaccg ctagaatagc tgaataaaca gcatttaaat ttgttccgat gactttggca
     1921 taatcttgct ggaataaaaa gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa
     1981 ccggaaagtc cagctaagaa taaagaaacg gaatctgctt tttgtttcat tagtttgcgc
     2041 agacctttat aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc
     2101 agcaaggctt tccagcttcc atgttctgtt ccgatgactg ggataaacgg agaaaagaca
     2161 aaagacaaag gtgtttggat aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg
     2221 ccaagaaaag cacctagtct tggtcctaaa acgatcgaag caatgattac aggaatatgc
     2281 atggttgttg cattgattgg accgataggg ataaaaccta aaggcgtaac agctaataaa
     2341 atcaaaatag ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt cataagtact
     2401 ccttttttct aagcatttct ttcattaaga gtcagcatga cctgttcgat gatcgtctga
     2461 atatcagcta aggcaccttt tccaaaatct ccacaagcta aaagagattc tcttggttca
     2521 atttcctgat agccgacaga tctcaatgtt tccagattac gctgagtgat cgggttttga
     2581 tacatattcg tgttcatggc aggggcaatc aattttggtg ttcccactgg aagcgccatt
     2641 gcgactgttg aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct
     2701 gtagcaggcg cgatgagaaa caagtcagct tgttttgcta gttcgatatg attgattacc
     2761 tgcggatttg attcttgcat gacgtctgta tggactgcgc gcttcgacag agattgcaag
     2821 gttaatggcg tgataaaatg tgtgctgctt ttagacataa tcacatctac ctgggcacct
     2881 aatttgacga attgacttgt aagatcagcg cttttgtaag cagaaatact tccagtaacg
     2941 cctagtaaga tctttttcat tttgattcct ccagtctaat tttctcagta atcaatgcag
     3001 caatctcaga tttactttga gcttcttcca ctgttccatc tttagataag agatagccgt
     3061 ggtgtgtctc gccatggatt tccgtcaaat catttgcgaa aataaaatca gttttgttat
     3121 ttatcagact attttttgcg actgccagaa gagattcctt tggtacatct actaatagtt
     3181 tgaaaccaat gatttttgct tttggctgcc attcacgtag ataagaaatg atttttggtg
     3241 ttttcttcaa aacgagcaca agatgctcgg tgttagaaga gatctttgaa acagtggtat
     3301 ctgtctgttc attttccgca aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta
     3361 ggttttttgc cagctgttct tctgaaaaac taaaagcagg agtaaaatca ctgactgcca
     3421 tactgtggat aatcgcatcg tagtgttctt ttttcgttag ttcttctaat tgtaaaaata
     3481 agtccagtgc agattcgatc gtataaatcg ttatattgga gtgtcttttc ggtttcagtg
     3541 cttgtttcgt agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa taatcagcta
     3601 atgcttgccc taatcgtcct gtcgaatgat ttgtaatagc acgtacttga tcgatttttt
     3661 cagtcgttcc tccagctgtg attagaatct tcatttatgg tcacctacat ttactagtgt
     3721 tcttccttgg tgtgttccag caagtagttt agatgctgtt tcaggcaatt gatccagagt
     3781 aatctcttgg acgaccagtt catctgcaat ctgcaaatcc gccatctgtt gccataaaga
     3841 aagacgttga tctattggta cattgacaga atcaattcca atcaactgga tgtttcgcag
     3901 aataaaaggc aagacagtcg cgtttagttt gattccccca gcattgccac ataaagctac
     3961 tgcaccgtta taagagatca atggaagaag actgcttagc tgctcgccac ctactgtgtc
     4021 gatgacatag tcgatttgct gttttcctaa agcttttgtt gtctctggca gaaattcatc
     4081 aggtcgtacg acttgcaaag cacctagact ttttagccat gtgacagact cttcttttct
     4141 ggaaaaggca atgattgact ggaatcctag tttatgtaac agggctatcg ctgtactgcc
     4201 tacaccgcca gaagcaccag taacgacaac ttttttatcg gaggtcatac cttgacggat
     4261 taaagcgttc acacacaaca tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg
     4321 cagcgtcata ttttttggta gaggaactaa ccagtctcct ggtacttttt gatattggct
     4381 ataaccgcct ggatgggtga cgccaagccc aaaaccagta gcaagtacaa gatcttcttt
     4441 tgaaaaacgt ggattgttac tttggacgac agttccggat aaatcaatcc ctggagtcat
     4501 cggatatgtt cgtatcacct gaccagactc gctcattgcc aatgcatctt tataattgac
     4561 atcagaatag gcaattttta ccaagacttc attttcagcc aactcagtaa atggcagatc
     4621 actgattgct gtatggaaaa caggttcctt tattacctga aaagatcgaa aagcgttcat
     4681 tcgtgagaca ctccttttaa aattttcttt tatcatacc
//
LOCUS       DASOUD010000142         4711 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.146, whole genome shotgun sequence.
ACCESSION   DASOUD010000142 DASOUD010000000
VERSION     DASOUD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.146"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            162..392
                     /locus_tag="U4A29_002482"
     CDS             162..392
                     /locus_tag="U4A29_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO0681579.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="U4A29_002483"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="U4A29_002483"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO0681580.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="U4A29_002484"
     CDS             1803..1979
                     /locus_tag="U4A29_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO0681581.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="U4A29_002485"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="U4A29_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO0681582.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2594..3061
                     /locus_tag="U4A29_002486"
     CDS             2594..3061
                     /locus_tag="U4A29_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0681583.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="U4A29_002487"
     CDS             3146..4030
                     /locus_tag="U4A29_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO0681584.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4144..4380
                     /locus_tag="U4A29_002488"
     CDS             4144..4380
                     /locus_tag="U4A29_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO0681585.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4468..4548
                     /locus_tag="U4A29_002489"
     tRNA            4468..4548
                     /locus_tag="U4A29_002489"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4502..4504,aa:Tyr,seq:gta)
     gene            4555..4626
                     /locus_tag="U4A29_002490"
     tRNA            4555..4626
                     /locus_tag="U4A29_002490"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Gln,seq:ttg)
BASE COUNT         1577 a          737 c         1028 g         1369 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaacaatt tggaaaatag cttcttatat tttgcaaaat tgtgagttat
     2461 tgccgaagtg ttggattttg aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac
     2521 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4501 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4561 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4621 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4681 accttgttcc ttgaagattc tgctgattta a
//
LOCUS       DASOUD010000143         4461 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.147, whole genome shotgun sequence.
ACCESSION   DASOUD010000143 DASOUD010000000
VERSION     DASOUD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.147"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            574..1008
                     /locus_tag="U4A29_002491"
     CDS             574..1008
                     /locus_tag="U4A29_002491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681586.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="U4A29_002492"
     CDS             2298..3221
                     /locus_tag="U4A29_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEO0681587.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="U4A29_002493"
     CDS             3237..3776
                     /locus_tag="U4A29_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO0681588.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1663 a          665 c          760 g         1373 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc gtctctatcc attggtagag
      421 gggctacttg gcatagtgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DASOUD010000144         4457 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.148, whole genome shotgun sequence.
ACCESSION   DASOUD010000144 DASOUD010000000
VERSION     DASOUD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.148"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(543..2048)
                     /locus_tag="U4A29_002494"
     CDS             complement(543..2048)
                     /locus_tag="U4A29_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO0681589.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="U4A29_002495"
     CDS             complement(2045..2731)
                     /locus_tag="U4A29_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO0681590.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U4A29_002496"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U4A29_002496"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEO0681591.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1250 a          922 c          731 g         1554 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatagca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaa
//
LOCUS       DASOUD010000145         4447 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.149, whole genome shotgun sequence.
ACCESSION   DASOUD010000145 DASOUD010000000
VERSION     DASOUD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.149"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            250..1098
                     /locus_tag="U4A29_002497"
     CDS             250..1098
                     /locus_tag="U4A29_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEO0681592.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1243..2184
                     /locus_tag="U4A29_002498"
     CDS             1243..2184
                     /locus_tag="U4A29_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEO0681593.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2174..4345
                     /locus_tag="U4A29_002499"
                     /pseudo
     CDS             2174..4345
                     /locus_tag="U4A29_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         1390 a          872 c          818 g         1367 t
ORIGIN      
        1 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
       61 taaaaaaaca taataagatc actttgcttg taggatgtat tcccttccat tacaatgaaa
      121 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
      181 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcggcaa acgttcaact
      241 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
      301 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
      361 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
      421 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
      481 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
      541 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
      601 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
      661 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
      721 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
      781 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
      841 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
      901 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
      961 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     1021 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     1081 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     1141 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     1201 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     1261 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     1321 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     1381 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     1441 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     1501 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     1561 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     1621 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     1681 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     1741 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     1801 cttttttgct tttggggttc gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     1861 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     1921 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     1981 acttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     2041 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     2101 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     2161 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     2221 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     2281 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     2341 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     2401 gacattttcc atattatgga tcttacagtt tttttcgtca gttacagagg aattcttcca
     2461 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     2521 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     2581 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     2641 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     2701 ccggttgttg tcgagaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     2761 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     2821 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     2881 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     2941 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     3001 gaagatcgaa aataaaaatc attacaccgg ttctggatga atagaagatc aggacgcacc
     3061 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     3121 caaagaaaac attacattga ccctaaaaaa acaagctttc ttaccttatc cttatggaaa
     3181 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     3241 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     3301 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     3361 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     3421 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     3481 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     3541 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     3601 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     3661 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     3721 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     3781 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     3841 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     3901 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     3961 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     4021 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     4081 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     4141 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     4201 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     4261 aaaaacctat ttagaaataa cgcatataat aagaaaaacg gtatacaaaa aaattctgac
     4321 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctcgaatgcc
     4381 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     4441 caacctt
//
LOCUS       DASOUD010000146         4425 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.150, whole genome shotgun sequence.
ACCESSION   DASOUD010000146 DASOUD010000000
VERSION     DASOUD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.150"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            690..1658
                     /locus_tag="U4A29_002500"
     CDS             690..1658
                     /locus_tag="U4A29_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEO0681594.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="U4A29_002501"
     CDS             1669..2484
                     /locus_tag="U4A29_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEO0681595.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="U4A29_002502"
     CDS             2552..3262
                     /locus_tag="U4A29_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO0681596.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="U4A29_002503"
     CDS             3298..>4425
                     /locus_tag="U4A29_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEO0681597.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DASOUD010000147         4219 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.151, whole genome shotgun sequence.
ACCESSION   DASOUD010000147 DASOUD010000000
VERSION     DASOUD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.151"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1257)
                     /locus_tag="U4A29_002504"
     CDS             complement(37..1257)
                     /locus_tag="U4A29_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO0681598.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="U4A29_002505"
     CDS             complement(1277..1888)
                     /locus_tag="U4A29_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO0681599.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="U4A29_002506"
     CDS             complement(1931..2863)
                     /locus_tag="U4A29_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO0681600.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="U4A29_002507"
     CDS             complement(3284..4144)
                     /locus_tag="U4A29_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0681601.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1198 a          853 c          690 g         1478 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgctt
//
LOCUS       DASOUD010000148         4179 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.152, whole genome shotgun sequence.
ACCESSION   DASOUD010000148 DASOUD010000000
VERSION     DASOUD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.152"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="U4A29_002508"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="U4A29_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO0681602.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="U4A29_002509"
     CDS             complement(2066..3064)
                     /locus_tag="U4A29_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO0681603.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="U4A29_002510"
     CDS             complement(3290..3943)
                     /locus_tag="U4A29_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO0681604.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         1218 a          889 c          652 g         1420 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacct
//
LOCUS       DASOUD010000149         4147 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.153, whole genome shotgun sequence.
ACCESSION   DASOUD010000149 DASOUD010000000
VERSION     DASOUD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.153"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..388
                     /locus_tag="U4A29_002511"
     CDS             50..388
                     /locus_tag="U4A29_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681605.1"
                     /translation="MVAILEMRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            392..754
                     /locus_tag="U4A29_002512"
     CDS             392..754
                     /locus_tag="U4A29_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681606.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            975..2381
                     /locus_tag="U4A29_002513"
     CDS             975..2381
                     /locus_tag="U4A29_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEO0681607.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            2828..3802
                     /locus_tag="U4A29_002514"
     CDS             2828..3802
                     /locus_tag="U4A29_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO0681608.1"
                     /translation="MSKRGTIVNEWEKLNRISNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     HEGKGRIDFNPNKINQFLSGSMKNFIHDLFLEPHFSRADIACDIVDIPDDFVSQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRESFAESADDLKRELDTWLQGLDVTEVGAE"
     gene            3799..>4147
                     /locus_tag="U4A29_002515"
     CDS             3799..>4147
                     /locus_tag="U4A29_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681609.1"
                     /translation="MSNPVEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQ"
BASE COUNT         1445 a          602 c          928 g         1172 t
ORIGIN      
        1 aaaaaaacga tcagttgtta ctagaaaatc aagaattaaa aaatcgaaaa tggtggcaat
       61 tctggaaatg aggtgtgaca tggataaaat tgatttgaga gaagaaacag tcgatagaat
      121 tgaattgatg tatgaacggt tagctgatta tttgaatttg ccggatgaag ataattatag
      181 tgaaatgctt gaacgaatta tggatgttct caactcggtt atcaatgctg atttagcaaa
      241 atcttcagtt ttgactaatg aatttgttca taagcattta gcgcaaacaa ctgatgaatc
      301 agaaaaagaa ttttggttga aattaatgag tcatgttatg cagttaaacc aaatttccgt
      361 tattaattcc aaagaaaagg agatgtagtt tatgggaatg actttagcag atattaaagc
      421 gcaagaagaa aaattaaaaa aagaacaagc gaaacttcgc caacagaaaa aacgcttgat
      481 tgagaaagaa aatgctgcaa tgggcaaggc tcttcgcaag cgttttgggt ttgaaacttt
      541 tgctgatttt gagcggtggc tatcgtcgat ttctgacgat cagcaacacg aaaaatcagt
      601 ggataatgag tggcttgctg atgaaaaatc agcgccaagc gaacgagatt atcccgatga
      661 atctgacggc ttattcggag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
      721 tacgtcaata tttaatgacg tgaatttacg ataagcgatt attttgcgtg aagcaaaata
      781 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
      841 gacagcgaat accctcggag agcatttgga attgtgacag ccatgtcaca attaggaaaa
      901 tgttactctt tgacaaagag taacagaaag gtttacactc tttttattag cgacagccga
      961 aggagggttt taacttgggg ttcagtattt cgttcaaaaa gggaacaaag aatacaagca
     1021 tttctcataa taatcgtgag ttaactgata gccaaaaaat caatgactgg cataagcata
     1081 ttgatttcac aaaatcggat gaaaatatct atttagaaca aacaaatatt cgtgaaaaat
     1141 atgaagaatt attcggtgaa gccgtagaaa aatataatgc aaagcagaaa agagccgata
     1201 gaaaaatcga ggactattta gcaaaggttc gcaaggataa aaaattggaa ccacagcgag
     1261 aatttatcgt gcaaattggt actcttgatg attttcgtac tactcgtgat gatggttctt
     1321 ctactggcat atcggaacaa caagcagaac aaaatcgagt gattgccaac aaaattttag
     1381 ttgagtattt caaagaattt aaagaacgga atcctaattt atccgtctac aatgctgtta
     1441 ttcataatga cgaagtaagt ccgcacctgc atttgaatat tgttcctgtt gctgaagggt
     1501 ataaacgggg tgttcaaaag caaccaagtt ttaacaaagc cttgcaacag cagggtttac
     1561 gagcagataa agagaatggt cgagtgattt ggaataattt taggaatcag gaagttgagt
     1621 caattgaacg tttgatggca gattttggtt gggagcgtga gttagtcggt actaacaaaa
     1681 tcaaagatgt tcacgaatat aaagaaatca tgagcgagat ttcagaattg cgtcaaacgg
     1741 ccgtaaaaga gcgtgagagc gtttctgatg agttgaggta tatttctagg caaagagaag
     1801 aaaatgagcg agaaaaggct ctggtggctt ctgagagagt gaagattgaa gaagagaaga
     1861 aaaatatgct gaatgtaaaa aaagagatgg cggtcatttt ccaaaaagat attgttcctt
     1921 tagaaagttt caagaggctt cctgatggtt cgtatcattt atctgggaat gattttgttg
     1981 atttgtatca acgagctacg agagcaacaa atgccaagag aaatgctgat aacatgagtg
     2041 agcaatattt acagatgctt gagcaaaata cagaattaaa tgagtatgta gctacattgg
     2101 aagaagagcg tcatttgtct ggtagaaaac aattagcaga tttacaagtg gaaaataaaa
     2161 agctgaaaga agaaaatcgc tctttacgcc agcgtttaga gcgattaaaa aacgcatttc
     2221 aaaaatcgat cactcgtttg tctatccgtt ttggtcttgc agaaaaggat atggggcttt
     2281 ctgaggaatt agaagtgtta gagcaggaga aaaccttatt ttatcgtcaa aacgtctcac
     2341 agcgtaaatt agagcgttct gaagatgaga tcgagtggta gttacgatgt gcagtcaacg
     2401 gtaccggagg cggtttgtgc cgaggataag gagttgacgg ctaaaccact agcctattaa
     2461 gctgacattt gctaaaaaat ggccagtcca ttttttggcc aacctagcgt tagcgaagtt
     2521 tgggggcgcc cttttcaaag ggtgcggttt tattttttgg atatttgcat agctaaagag
     2581 aagtgctatt attgagttag caaacaaatg aactgaaaaa agagtaaaaa aatagtcgag
     2641 caacgctacc aacgttctcg acctttacgg aaactaatcg cactagctcc gtaacttctt
     2701 aatcattata cgaatatttt tagatttctt caagactaga gattttttgg taatgaattt
     2761 ttaagagtta cgtgaacttt tgaaaattct ttccagaagt ctctttttct ttgaggagga
     2821 acaaaatttg agtaaacgtg gcacgatcgt gaatgagtgg gaaaaactta accgcatttc
     2881 taacaggcgg ttaaagtatc aaagtttgac ccttccctta aaaatgcagt ggtcaattga
     2941 ccgaattacc atagtcggaa aactgaaaga aaatatttat tatcacactc caaatgatgt
     3001 gttgatttta aattttgaac agcttatgcg tttgaacgaa ggcaatggtt atttaaaagc
     3061 tgttgggaat aatgggtggc aattattaga ccaacacgag gaaaatatag cttatattga
     3121 gattcttaaa tttcatgaag gaaaagggcg gattgacttt aatcctaata aaatcaatca
     3181 atttttatca ggttccatga aaaatttcat tcatgattta tttctagaac ctcatttttc
     3241 acgtgcggat attgcttgcg atattgtaga tattcccgat gactttgtta gtcagtatcg
     3301 tgtagttgat cctgtgtctt ttaaaccaat ttatgggcgt tctggtaagt tagaaacggc
     3361 atattggggt agtcgtgcca gtgaacgaca aattcgaatg tacaataaaa aattggaaca
     3421 agaaagaaaa cgtaaaattg ttccgccgga aataacgaca tggtggcgac tagaattgca
     3481 gttacgaagg ggaaaagcaa ctgattggca tgagatggtt catgaaagtt tggatagctt
     3541 tgctagtcca cattattttc cgccaaatac taaagttact gaacggatta tggtgaaagg
     3601 tttgctttat gatcattctg agtggggaaa tctagctaag aatacaaaga aaaaatacag
     3661 ggatttgttg aagcaagaaa gtcaaaatga tgaattgacg aatcatttgc gagagtcatt
     3721 tgcggaatcg gcagacgatt tgaaacgaga attagataca tggctccaag gacttgatgt
     3781 aacggaagta ggtgctgaat gagtaatcca gtagaaaaaa tgaccataaa aaatcttgct
     3841 gacgaattgg gtgtatcgaa aaatacagtg cgcaatcaac tggtcaattc agggtttgac
     3901 cttgctcagt ataaagaaaa tggcattatc atgctagata aagagttgat tggtgttgtt
     3961 cggtcaattt atcggtcaaa aagtgatcaa ttgactggtc aattaccggt caataacaca
     4021 ggcagtgatt ttcctttaga aaaagagaat gagtttttgc gtgatcaatt agctgagaag
     4081 gacaaacata tttctgaatt aacagaaatg attcgtgatc aacaaaaatt gaccctgctt
     4141 cagcaac
//
LOCUS       DASOUD010000150         4041 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.154, whole genome shotgun sequence.
ACCESSION   DASOUD010000150 DASOUD010000000
VERSION     DASOUD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.154"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            179..775
                     /locus_tag="U4A29_002516"
     CDS             179..775
                     /locus_tag="U4A29_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO0681610.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="U4A29_002517"
     CDS             899..2698
                     /locus_tag="U4A29_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO0681611.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESMKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="U4A29_002518"
     CDS             complement(2976..3188)
                     /locus_tag="U4A29_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681612.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="U4A29_002519"
     CDS             3650..3799
                     /locus_tag="U4A29_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681613.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1189 a          689 c          711 g         1452 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagta tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DASOUD010000151         3925 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.155, whole genome shotgun sequence.
ACCESSION   DASOUD010000151 DASOUD010000000
VERSION     DASOUD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.155"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..764
                     /locus_tag="U4A29_002520"
     CDS             <1..764
                     /locus_tag="U4A29_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681614.1"
                     /translation="KKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRL
                     NGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLI
                     APEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDN
                     SNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLA
                     IASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(909..1649)
                     /gene="truA"
                     /locus_tag="U4A29_002521"
     CDS             complement(909..1649)
                     /gene="truA"
                     /locus_tag="U4A29_002521"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO0681615.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1840..2475)
                     /locus_tag="U4A29_002522"
     CDS             complement(1840..2475)
                     /locus_tag="U4A29_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEO0681616.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2622..3863)
                     /gene="sstT"
                     /locus_tag="U4A29_002523"
     CDS             complement(2622..3863)
                     /gene="sstT"
                     /locus_tag="U4A29_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO0681617.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
BASE COUNT         1339 a          801 c          589 g         1196 t
ORIGIN      
        1 taaaaaaatt aactgctgat gatttattta ttttctttct tggtgtattt gttctttttt
       61 gttcaaccaa tttatttcct tggcaacttt ttaaccatac accagttaag agtctacaat
      121 ttgtctggcg gttaaatggt ttttcttctt tatttatagc ctatacgatg agtatagtga
      181 tctattatat tttttctact aaaaataatt catggaaaat aatggtcttt acgtttcttg
      241 cactaatatt gcatctctca ggagtaagca attctatcca tgctaacaaa gattcattaa
      301 cacttatagc acctgaagac gctgttgcta tagctgaaaa ttacaatcat actgattatg
      361 caaacaaaga gtcaatctat catcctgata tgattaataa tgatcaatat ttactaggta
      421 accaggaaat aaatccaaca actcatttta tgagtaacaa acttacaatt gaattagata
      481 attcgaataa taaaaatact gtcttgacga cacctattta tcgttataaa gggcaagtgg
      541 cttctattaa tggcaaacta gtacaaacaa aattatctaa gtttggcaca actgaattaa
      601 ctattcctcc aggtataaat aaagtcgtta ttacttatca gtacacaaaa ctagcaattg
      661 cttcaagata tttatctatc gttactttga ttttattttt actttatcga tttacttttt
      721 ctaaacaaaa acaaccgaga agagaagtac aacattctca ctagattttt atttaagaaa
      781 gactttttag ctgtacacat aaacaggcta aaaagtcttt ttttatattt ctttaatttg
      841 cttcatacat tatttaacat cgatatttat actgtgaaac atgcgtttca tccattatca
      901 aagtattttt atcgataata aacttcatcc aaaaacagac cttgtgcagg gacggtttct
      961 cctgcatgtt gccgcacttt gctttcaaat atttcatcga ttgtttctat cggcatggca
     1021 cctgtgccga tttctagtaa tgtccccatg ataatccgca ccattttata taagaatccg
     1081 tctccgacaa aagtaaaatg aagcatttct ccctttcgtt cgatagataa ttcatcaatc
     1141 gtacgcactg tggatttttt tgattttttc aatgcagaaa aaccaatgaa atcatgtgta
     1201 cccactaatt tttcgcaagc tgcattcagc ttgtccatat ccaactcttg agggaaataa
     1261 aagctgtggt ttcgctcaaa tgctgaagga atcacgttgt tccaaacata atagctgtat
     1321 tttttgccca ccgcattata tcttgaatgg aagcgctcag gcatttcttc gattcttttg
     1381 atgacaatat cccgcggaag gtaacgattc ataaagtcga tcatttctgc cagtccaatt
     1441 tctgtgtttg ttttaaaatt tgctacttgt cctcgcgcat gggtgccagc atctgttctt
     1501 cctgatccaa taatttctat ctcttcattt gtcatctgat gcaatacttt ttcgattttt
     1561 ccttgaatgg ttttatcgtc atctccaagt ctttgccatc ctgaatatct ttttccgtca
     1621 tattcgattg tcaacttgat gtttctcata ttttagtggc tcctctgtct tccatttggt
     1681 cttctctcta cctttttaag attgtagcac aaaatatagc tccttgcttc tctaagacag
     1741 aagctataca agaaaactgt ggaaatatat atttcagaaa aacaaaaaac agccaccgga
     1801 tgaagtatcc gctgactgtt caatttgacg gctcaagttt tactgtttga gatgcaagat
     1861 actcaaccag gcaatgggca cataattaaa gaaaaaactg attctttggc aaaaacctat
     1921 tttattcagt aatggccaag aatttataga aggaattctt gccagtccat aaatcgccgc
     1981 aaaaagcaaa ctgatcacag acagtccaag gtataagtgg cttacgtcca tttgcccatt
     2041 ttttcgatag atcaagaaaa gaagaactgg aaacaataaa aaaccactgt aacctaaacc
     2101 tgatccgata ttatggaccc atgtcgagac attccacgaa gattcatggg tattgatact
     2161 gaacaaacct gtaaagatac aatcacctat accatagaac gcgatggctg ctgtaactag
     2221 ggtagctaac acacgagatg tactaacaaa tgcagcatac aatgcaggca atgacaggat
     2281 gaaaaagatc cctgaaacca cagaccaaat aagaaacgct tttcttacag gactggaaac
     2341 ctctccaaat acactgatca cgctcttcat ttgatctaat tcaggataat acaatcctaa
     2401 tacgtacgga gtcaaaaaat cactcagcac gcctaataat aaaaagaaaa aaccaaattt
     2461 acgtaaaaaa tccatgatac gcctccaata acaattacct atgcttttaa ttgttattat
     2521 aaacgacttt atcttgattt aacagaaacc tctatttaca cgaacatcta tataactcat
     2581 taaaaactcg aatcatttta aaaatactaa atatctaacg ttcaacgttc acttaatcca
     2641 tctaaacaat ctttcaattt gattttttcc ccacttcttt tacgcgcgcc tagctctcct
     2701 attgcagtaa acaacagatc acttgacgaa tttaaggcag tttccattga atcctggata
     2761 acaccgataa taaatccaat accgacgatt tgcatcgcga catcattaga gataccaaac
     2821 aaactacatg ctaatggaat caataacagt gaaccgcccg caacacctga agctccacaa
     2881 gcagctaaag cagacaaaat acataaaagg actttcaaaa caaaaggtgc ttcaatccct
     2941 aaagaatgta cagcagtcaa cgtcatcaag gaaatggtaa tcgcggcacc gcccatattg
     3001 atcgtagcac ctaacgggat cgatatggaa taagactctt ttgtcaattt caatctttca
     3061 gccaacatca tgtttatcgg gatatttgcg gcagaacttc gggtaaagaa agcgggaagt
     3121 gcgctttctt tcaaacaaaa gaaaatcaat ggatagggat tttctttaat aaacaaaaac
     3181 gtgaggaatg ggtacacaac taatgcgaca aacagcatcg taccgatcaa aacaagtaaa
     3241 agctgcagat actctgccaa tccagcgatt ccagtcgtag caattgattg ataaaccaat
     3301 cctaaaatcc ctatcggagc aaatgcaata atcacttgta cgacttgtga caacgctata
     3361 gatatctgat ccaccaactc ttttgtcgat tctgaacgtg ctcgaaaagc tagtcccaac
     3421 aatacagccc agaataagac gccaaggtaa ttcgcatcaa taagagattg aacaggattt
     3481 tgtgtgacat tcgtcaacat agtagataaa acactttcca aactttgcgg tgctttttca
     3541 gatactgttt cttgtaaagt aataggaaca gtaaaaattg aacttacaat aactgctaca
     3601 agtgcagaaa gcaatgttcc cactaaatat aagatcaaaa taggcttcac gaatgtctta
     3661 ttcccaattt tatgcttggc aattgacgcc atcgtcaaga aaaaaaccag aataggtgcg
     3721 attgccttca agcaactgac aaacaacgtt cccaatattg aaataaatgt ccatgtgggg
     3781 aaaaagatac caaacaatat tccgactaaa ataccaacaa caatacgttg aactaacgac
     3841 atatccaaaa acttctctaa caaatgacca ccctttcaca attttaacaa tcattgtatc
     3901 attttatgca aagaaactaa aggta
//
LOCUS       DASOUD010000152         3921 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.156, whole genome shotgun sequence.
ACCESSION   DASOUD010000152 DASOUD010000000
VERSION     DASOUD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.156"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="U4A29_002524"
     CDS             7..624
                     /locus_tag="U4A29_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681618.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="U4A29_002525"
     CDS             1644..2813
                     /locus_tag="U4A29_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEO0681619.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="U4A29_002526"
     CDS             3286..3840
                     /locus_tag="U4A29_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO0681620.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT         1471 a          499 c          643 g         1308 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaacc
     3181 ccttgatttt cttcactttt aacagagtat atatataagt tcatgattat ggtaaaatat
     3241 atatataatc atgaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccagaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagacata cgaggtaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga gattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccaa gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatgtag cagaaaaatt aggaagagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggggca tcgttacgtg gttttaaatt gggaggctaa
     3841 ttgagatatt aggatatatt ttttgtgtat aatgaaaact tagaaggtga gattaatgaa
     3901 gataactgaa ttttcggaag t
//
LOCUS       DASOUD010000153         3896 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.157, whole genome shotgun sequence.
ACCESSION   DASOUD010000153 DASOUD010000000
VERSION     DASOUD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.157"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            289..1104
                     /locus_tag="U4A29_002527"
     CDS             289..1104
                     /locus_tag="U4A29_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681621.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1230..1487
                     /locus_tag="U4A29_002528"
     CDS             1230..1487
                     /locus_tag="U4A29_002528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681622.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1739..2311)
                     /locus_tag="U4A29_002529"
     CDS             complement(1739..2311)
                     /locus_tag="U4A29_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0681623.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2327..2932)
                     /locus_tag="U4A29_002530"
     CDS             complement(2327..2932)
                     /locus_tag="U4A29_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEO0681624.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3333..>3896)
                     /locus_tag="U4A29_002531"
     CDS             complement(3333..>3896)
                     /locus_tag="U4A29_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEO0681625.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT         1294 a          715 c          582 g         1305 t
ORIGIN      
        1 aaaaataaga aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca
       61 gtttcttaat agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa
      121 tttattaatc gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata
      181 attactatct agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag
      241 taaaaaatac tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata
      301 gcaggacttt ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac
      361 tggagacagt tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa
      421 aaatatttac cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag
      481 aatgaaggcg gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca
      541 actaaaacta taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata
      601 gacgataacc atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta
      661 gcccttgata gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac
      721 ttggttgcag tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac
      781 gttccttata acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat
      841 aaatttatct tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt
      901 aagatttatg acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca
      961 aataatctaa acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt
     1021 tctgaaaagg actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag
     1081 aaagaaaaca ttactgtact ataactagca tcggtccatc attaagtttg gactattaga
     1141 acacttgcta ttacagaaaa aggtatgcta gccacgctta ctatttattg gtatcaaaca
     1201 agtatcttaa tttgaaagga agatgattta tggctaattt atatatagag aacaaaggag
     1261 acttgaatgc cattattact ttaatcgtag ttatggtaaa atcaataggt aatatcacaa
     1321 taggaatgct gtttattata ttagcagcag tgctgatttt tatgatagtt atttggttct
     1381 taaatgaaag atctaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
     1441 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
     1501 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
     1561 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
     1621 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
     1681 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
     1741 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
     1801 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1861 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1921 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1981 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     2041 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     2101 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     2161 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     2221 tcttctctac gccgttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     2281 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     2341 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     2401 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     2461 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     2521 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     2581 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     2641 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     2701 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     2761 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     2821 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2881 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2941 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     3001 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     3061 ttgcacagtc tagttgacct ttgatacacc tctatattta tagagaatct aaaacatcaa
     3121 catggcatac tcaaaattct aaatattagg gataaagaat tctcctatac aatcaaaaat
     3181 ttcctttatt tcgaactccg gtcacctaac tctattgatc tcttacaagc caaagaatat
     3241 ctctatacta caaagaaaca atagggtgcc tggttggtct ctttgtgttt tattttagaa
     3301 tctagaacgt aaatattctt gtcattctga ttttatctaa gaatatcaat taaatctttt
     3361 tttgcagcct ctgaagcggg cattacagaa aagaccagtc caataaccga agatacccca
     3421 accgctagta aaatagtaaa tagatcaagt gacacgttta cctttatcaa agaaccaaca
     3481 ctataagcaa gagccattcc taataaatag ccaattaacc ctcctgaaag agttaaaatc
     3541 aaaccttcta aaaggaactg cagcataatc gattttcgag tagctcctag ggctcttctt
     3601 ataccgatct cttttgttcg ttcagataca gatatataca tcatgttcat tacacctacc
     3661 cctgcgataa ataaagatat acctgctaca gctgttataa aataagtgat cgtactgagg
     3721 atttgaccaa ttcctttcgt taacatagcc gtatctaaca cttcatattc ccccaaatgt
     3781 ttcaaactac ctttttcttt caattggttt atcactttag aggtcacctt gtcaggttca
     3841 actccttcct ctaaagttaa cgttagagac gatgtatcct tttcagactt aaaata
//
LOCUS       DASOUD010000154         3865 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.158, whole genome shotgun sequence.
ACCESSION   DASOUD010000154 DASOUD010000000
VERSION     DASOUD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.158"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(247..468)
                     /locus_tag="U4A29_002532"
     CDS             complement(247..468)
                     /locus_tag="U4A29_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681626.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(566..811)
                     /locus_tag="U4A29_002533"
     CDS             complement(566..811)
                     /locus_tag="U4A29_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681627.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1051..1665
                     /locus_tag="U4A29_002534"
                     /pseudo
     CDS             1051..1665
                     /locus_tag="U4A29_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2035..3036)
                     /locus_tag="U4A29_002535"
     CDS             complement(2035..3036)
                     /locus_tag="U4A29_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEO0681628.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3195..3461)
                     /locus_tag="U4A29_002536"
     CDS             complement(3195..3461)
                     /locus_tag="U4A29_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0681629.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3451..3807)
                     /locus_tag="U4A29_002537"
     CDS             complement(3451..3807)
                     /locus_tag="U4A29_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO0681630.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1433 t
ORIGIN      
        1 ttttactata tacttcttcc cagaacttgt ccgtctcctt caaattatct ctacctctcg
       61 agttcattgg gtggtttgcc catttattaa acatggaatc acctttctca tactcatata
      121 tttatgtcct atttcaatta tatttatata tgcttaaata aatttgtatc ctgaaattta
      181 ttgtaatatg aagctgtatt ttacatatag taaccaaaga gttaataaaa taaaaagagc
      241 gaaccattat agatcagctc ttttttcatt gctgttttta atcttatcta gaatcacttc
      301 aaatgtttca aaggtattta catctaactg acataaccaa agcaagtact tcttttcttt
      361 gtaagtccaa tcaatattac ggtaagagga cattagatta tggtaccttg ctctttgttc
      421 aactgaagcc aattcccagc gtttttcaaa tattttttct tcttccatta ttgatccctc
      481 gcaagacgct acctcaaact taaataatgg gcagcgaaag tctaattcct tagatctaag
      541 tatagcaaac aaatatgaaa aaaacttaga aggtagtaaa agttcttccg tcttttgctt
      601 tgcttctatt cttaatgtgt ttcgttatcg gtgccctaca aagattttca ttaattacac
      661 actcatttat tgttgtgtat tttctgacat ttctctcttg atcaattatt ttaattccat
      721 gggcatcctg atagttcata ggagaggagc ggctatcaga aatagttgca cctatctctt
      781 gaacttcttt tacaacttct ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata
      841 atctataata tttatctttc cttttcaatc taagaaatcg tcaccccatt ctttttcttc
      901 tattataaac tatttaactt ttagaattca aaaaccgttt gtctatttag agtttaccct
      961 aaatagacaa aaatataaca ttgaaaaatt tagagttcta acgtatgatt agggtgtatt
     1021 ataaacgtta tagaaaagta gggaataaat ttgaaaattg cttatattag agtttcatcc
     1081 atcgatcaaa atgaacaacg tcaaattgaa gaaatgaaaa agtttggcgc tgaaaaaatt
     1141 tttatcgaaa aacaatctgg agcgacgatt agtcatcggc cgattttcca ggaagcttta
     1201 gattttgtca gagagcaaga tatttttatt gtagaagcca ttgatcgact tggacgcaat
     1261 tatgacgaga ttatcgattc agtgaattat ctaaagaaga agaatgtgcg acttattatc
     1321 accagtttac cgatcatggc tgaagcaatt ggaaatcctc tcctagataa attcatcaaa
     1381 gacttaatca tttaaatctc agccatgatt gctgaacaag aacgtactga atcgaaacga
     1441 cgccaggcac aaggaattaa aatcgctaaa gcaaacggcg tctataaagg tcgtcctaag
     1501 ttatacagtg cagacaccaa agatcctcaa cgtcgccttg tctataggag tatcgttcag
     1561 gatcttgaga atggggttgc tatctcaaag attgcaacag actacaatgt gactaggcaa
     1621 acaatttacc gaataaaaaa agaaatcgac caactaattg tatgatttct tttttgaatc
     1681 acgtaattat taattagctt tgtcatcaat acagacaatc attttagatt catctagcta
     1741 agttgattac atttatgtta aaaagaattt agttaacata aatataattg atgcataaat
     1801 cgtccttatt taaaggcatt taacattgac aacttagtat aatgtatctt atgtaaacta
     1861 ataagatttg aattttattt tatacacact cgataataga tcaattttat ggtcccccgc
     1921 atttacgatt gcacaagaga aacataaagt cgacctatta gcatttcttt tcaattcaaa
     1981 cattaaaaaa gtttatacct ctgctactaa aaaatgaaat tatctattta aaatttaaaa
     2041 cggtaaatct gattcatcca aattggaaaa tggatcagcc tgtattggag ctttgtattt
     2101 ctccataata acagatatct ttttttcatt ttttaaaaaa taccttttaa atttcttata
     2161 gtcctctgaa atcaaaatat agcgtgatgg atactcatca aaagaagaat cgtattcttc
     2221 ctttaaaatt cccgatttac ttaatttttc tagtacatca aatactctat cactcaagta
     2281 actccttaaa ttgttatcac tttcccattg aactactttt ctataagttg aattatcagt
     2341 tgaaaagata gaattttcat ccataaacgc atatattagc agcaatcttt catcattgtc
     2401 aaattctatt ggatcaaaaa tatctaagtt gtcatcaaaa tctttatcag ttccggtaac
     2461 ttttttgtta attactgaag gtgtcttgta agaaatagaa tctaaatcaa agcgttttga
     2521 ttctaaatta ttttgtgctt tctcaattaa ataattcact ttttttgtga gaaaccttct
     2581 gatattgtct ttgctatatg ttccataaaa acccttatct tctaaagagt ccctgtactt
     2641 cttaacttct atatactctt cagccaaaac ttgtggaact ggttcatcta agaaaaaaac
     2701 ccctacctcg cctttgctat ttttataaaa gtaatcaatt tctgctatag ttcccgatct
     2761 ataattatca actggagaac ccaacttatg gccaaaaatc gctaataata tatcactatt
     2821 ttctacaatt tgtttattaa taacactttg cccagagcta ttatttatat aagtaggaat
     2881 cgcattcgtt tcccatcgaa ttggcatcaa aattactttt gtattatctg tattttctat
     2941 attccatcta tatatggttt cttctacttc attcctcttt gctgctatat cacctggtga
     3001 tgctataaat acttttatta gatttatggt tttcatttat tgtacctttc tctttatgtc
     3061 tctttaaagt aatttttgta taaaatttat tgacttttac actataggac aacttttatc
     3121 tatttagtac aaaaaggccc actaaactaa ttgattagtt aggagcctat tacaggtata
     3181 ttttcaactc gcttttaagc aaattctact accagctttt tgcctaatgc cctagcgata
     3241 tcatcaagtt tatcgaaaga tacgttatcc cctctctcaa ttcgagcaat agtagattga
     3301 gttgtatgtg ctttttgtgc aagctccgct tgagttaggc ctgcttcctc ccgtgcatga
     3361 taaagagcaa ccgcagtttc gagttttctt ccttcacggt catatgcttc tgcaaaatct
     3421 ttgtcttttt gcttttcttc aatcaatttt ttaatcatca tttgtgaacc actcctctct
     3481 aagttctttt gcatgtttaa gttcacttat tggtgttttc tccgtttttt tagtaaatcc
     3541 atgggtaatg acataccttc cattgaccac atggaaatag atagctcttt ggatgtttga
     3601 tcctacctta cttcttaatt cgtacaaatt agtgtctaat tttttcaccc atttcaaccg
     3661 ttgagctact agcatcccat tcttttctgt ttcttctatt gtacgtaaaa gcttcgcact
     3721 atcttttttt ggtaatgttt ccaaccattc taaaaattca tcatgaccat taggtctttt
     3781 aaatgtttca aaagatattt tttccatacc caattatatc atatattgat tttatattat
     3841 agtatataac ataaaaattc gaatt
//
LOCUS       DASOUD010000155         3849 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.159, whole genome shotgun sequence.
ACCESSION   DASOUD010000155 DASOUD010000000
VERSION     DASOUD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.159"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..345
                     /locus_tag="U4A29_002538"
     CDS             <1..345
                     /locus_tag="U4A29_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681631.1"
                     /translation="IIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKP
                     PKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPN
                     KNNNFYFYRNKL"
     gene            complement(471..1148)
                     /gene="nth"
                     /locus_tag="U4A29_002539"
     CDS             complement(471..1148)
                     /gene="nth"
                     /locus_tag="U4A29_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO0681632.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1155..1853)
                     /locus_tag="U4A29_002540"
     CDS             complement(1155..1853)
                     /locus_tag="U4A29_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO0681633.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2121..3305
                     /locus_tag="U4A29_002541"
     CDS             2121..3305
                     /locus_tag="U4A29_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO0681634.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFYQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3311..3754)
                     /locus_tag="U4A29_002542"
     CDS             complement(3311..3754)
                     /locus_tag="U4A29_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO0681635.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          999 a          759 c          636 g         1455 t
ORIGIN      
        1 attatttttt ctgtactttt aagcactact cttaaaggaa aatttatgga aagaagctct
       61 catgcaatga ttctcacttt tttatggttg ttgttcacca gaatctatat ttttctgatg
      121 agcctgaaac cgcccaaatc cattagttta tgtttagtta taaatggtat ttttttatta
      181 tcacttatca tttttattac acctttagat agatacgtca cttatttata taatcctttg
      241 gaatactgga cttattttat aggtatattg gaaggcatta ttttctacat cgggtatttt
      301 cccaataaaa ataacaactt ttatttttat cgcaataaat tgtaataagt taccatccta
      361 gctactgagt atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc
      421 cataataaca agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt
      481 ggtttctttt ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt
      541 gatagcaaag gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa
      601 aaaatcagtg tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt
      661 tcttctactt ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt
      721 tccacgtggg tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt
      781 gctgtctttc tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc
      841 ccatcgaaac gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga
      901 tacaaaccaa tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca
      961 tcgggtgtcg gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc
     1021 gcctgggcac ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct
     1081 ccatgtgctt cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg
     1141 cttaacatat tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct
     1201 tgttgagctg actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct
     1261 tcttccactt gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca
     1321 acgtaattaa aactataagc ctgatttaat acggcttccc gcagtgctag cttcaaaatt
     1381 tccggctgat aatggtcttc ttctagccat tgtccgatcc gctggaactc gatcgaagaa
     1441 agcgggcgtc caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt
     1501 gttttttctt cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg
     1561 gggtaaagat tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata
     1621 aatccatttg ttacgagacg atccagtatt tgaaaaatct gatcttgttt gattcccata
     1681 tccatagcaa tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac
     1741 tgtagaacaa ataaaaattc ttcagattgt agaccgatcc tatgataatt tttaagtaaa
     1801 agattagata tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct
     1861 cctccgctca atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc
     1921 tatttacttc actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat
     1981 gtttttgatg cgtatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct
     2041 tagaatgttg acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta
     2101 ttgcataaag gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat
     2161 acgtctctgt cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt
     2221 taacgctgcc ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt
     2281 ctgtttatgc tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt
     2341 ttgggcgtca tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt
     2401 ttggtttagg aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg
     2461 caggagaaat ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa
     2521 ccaaagtttt tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta
     2581 tagggggtct attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta
     2641 ctttcttaaa tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac
     2701 gttcagtaga tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca
     2761 agatttcatc cgttattcct ttattgacag caggttttgc tgtttggttg gcagctggct
     2821 ctcttcaatc tatcttttat caattttcga ttgacgcctt tcagtggaag gctgggttgg
     2881 ttggtttaag cttctctttg attggaatct tggatactgt ctcgcaattg ttcattatgc
     2941 ctcgattgct caaaaaattt tcagaacagc agatcacaag aatagggatg ttcagtgaaa
     3001 tcctcgccta tctattcatc acattatctg gaattcttct actgccgatt ttgtttttat
     3061 ttgggattat ctgttatggt ttcggtgatt ctatttttac tcctgtattc aatggccaac
     3121 tatccaattc tgtctcagat aaccagcaag gtcttgtaat gggcggtacc caaagtatac
     3181 aatctctatc gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct gcagcaccat
     3241 ttcttccgac ggtattcgga tttttgctta tgatttttgc ctattatcac tacaataaaa
     3301 aatagtagct tcatttgcta cttcgtataa attctctgta tcgaccgata aaacgaaata
     3361 ctgtgtcaaa ttcttctttt gacatatttt caaatacagt agcatctcgc tgtacccatt
     3421 tttgatggag ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc aattgaaaat
     3481 agatttcttt tttattctct tcgctttgat aagtgtttat gatccctcta gctatcagtt
     3541 tttttgttag cttactgatg gctcctctag tcatgtaact agcattcgct aattttgtca
     3601 cgttaggatc agggattttc ccgatatgtt caatcacttc tagttcattt agagtaaatc
     3661 ctgcaaattc tttttccatt tctggtttat tacgatgaat aatctcatta gctaaatcca
     3721 gatattcttc tattagcagt tgtcttttat ccatttctac accttctttt tctttcatta
     3781 tattgttttt ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa aacccaatct
     3841 gaaaccaag
//
LOCUS       DASOUD010000156         3771 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.160, whole genome shotgun sequence.
ACCESSION   DASOUD010000156 DASOUD010000000
VERSION     DASOUD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.160"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            404..610
                     /locus_tag="U4A29_002543"
     CDS             404..610
                     /locus_tag="U4A29_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEO0681636.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(812..1813)
                     /locus_tag="U4A29_002544"
     CDS             complement(812..1813)
                     /locus_tag="U4A29_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO0681637.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1818..3730)
                     /locus_tag="U4A29_002545"
                     /pseudo
     CDS             complement(1818..3730)
                     /locus_tag="U4A29_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
BASE COUNT          999 a          766 c          587 g         1419 t
ORIGIN      
        1 ttcttatttt ctaaaaaatt aaaaaaaatt ttggtgaatt aagtatttcc ttgataacac
       61 tggcttttgg cctcatttta ggttatcatt tgtaatttta tttcacagtt gatatgattg
      121 ttatgtaaaa taaaaaagtt taggatacat cgaatttttg gggaaattct tttgggggta
      181 tcctaaatct tttctattat attcccatat tttctattat tggtctagac gtatgatggt
      241 aaactttgta cctatctgat tgctgaatca ttaattgctt atcattagcc atctttgttt
      301 caagttcgct agatgatcat tttattcaca tcctaacaaa cttgtcacat ttatagattc
      361 ttaatctatt tatcatacaa ttataaaaaa ggaggtgctg cttatgaaag ctatccatat
      421 ttctttgctt tttttcgcta gtgcattgat tttatttgct attcatcttt gttcccagcc
      481 ttatatcaat aacaacggaa tattagtgga accagcattc ttttgcttac cactcggctt
      541 tttgtccctt cttgcctccg caggcttcgg atttgttgct ttttttaaac gatcaaaaca
      601 acaaatctga cttataaaat ttacgtaaaa gagatcgtga caataatcgt gtgatattcc
      661 caaatttcga ataaaaggtg ccccttgcca tctctaactc ctcagtcaca agttacaata
      721 ttcataaacc atgaaaagct tgtttattga aattgctcct tgtgacttgg agctaagtga
      781 cagattacaa cctcttttgc atcataagac tttactcctt gaaaaacatt actaactttt
      841 gatatgtttc ttgcgcttgt ttattgctga aatcaaactg atattcatgg ttcaaaacag
      901 catttttctc cgcataatct acttttgtga ctttcacccc cgctttctca aatacagaag
      961 ttacttcttc catttgtttc gtaaacgtat ttgtatttcc atccgttaag aatgtgtttt
     1021 gcgtccaacg atccgcatgt ctgaccaagg ctaaactttt gaccttctct gattgttcaa
     1081 agtccttatt atcaaagtaa gcccatccac atttttcata aagaaagcta gagattttat
     1141 cacttacttg agtaagtgca gaaggatcca acagaccaga caaagaaata aacttcgtaa
     1201 tctgcttgtt ctccaaagtc ggatcaagat gatcagcttt tttaatttct tcattttgaa
     1261 gtgctaacaa atattctcca ctaatttgtg cacctgccga atttccacca atcgaaatat
     1321 tcgaccaatc tgcttgatct tttatctctt ttttagctgc ttgaatggct tcgttcaatt
     1381 ggataactgg tgtaggatgt tttgcttccg gtgatcgttc ataatctata tttacgatga
     1441 tttgctgcgt gtctgaacat agatattcca aataatcacg acaatcattt ttatcccctc
     1501 ctacatatgc gcctccatgc atccagaaaa gaatcggtaa ttttttcttt tccactttcg
     1561 gtgtgtagat atccatatta ttatttttgt actttgaagg ataggatatt tcttttttta
     1621 tatcgatttg ttcgattttt tgctggaaat catctggtcg ggcatatact tgttcttttt
     1681 cacttgtgtc aaattgtttt ctaacaagca agctaatcgg ctttggtgtg atttgattaa
     1741 tcaaaaatac agtgactaaa ataactacga tgataactgg tactgcaatc actattcgct
     1801 tgaaccattt cattcattta ccctctcttt cttcgccaaa tccatgtgat aatcgtgcta
     1861 acagataaaa atccacctga ataaatgaga agcgaatgtt tcttttctcc agttttcggc
     1921 aacattcttt ttttgcatct gtcttttttt gtactttcac tgtcatctga atcggttgcg
     1981 tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct gcattctctg
     2041 tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa ataatagttg
     2101 gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat tcgtatcctt
     2161 cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc tggatttcgt
     2221 cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt atctgatcat
     2281 ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg tcaacttcct
     2341 gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct cctgataact
     2401 catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca tggataatca
     2461 atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta tccaaatgca
     2521 tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca tgctcttgat
     2581 ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct actgtctggc
     2641 tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca ataattcctt
     2701 tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct cccatcgaac
     2761 caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt atccagtaaa
     2821 ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta ttaccagtcg
     2881 ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg tattcttctg
     2941 taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact gttttatctg
     3001 aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt ttgtttagta
     3061 cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta ggcgttgtcc
     3121 catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa gcgatatcgt
     3181 acaatagata agtagcaatc agatgtttga taatctcttt atctacacca taatcattaa
     3241 cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct gttggtccat
     3301 taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca ttgaaatttt
     3361 cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact ttagcagaag
     3421 aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg ttatctgtgg
     3481 tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt tcagtatttg
     3541 tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt tctgtggttt
     3601 ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc cattgcggga
     3661 cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt tttgttctca
     3721 agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa c
//
LOCUS       DASOUD010000157         3716 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.161, whole genome shotgun sequence.
ACCESSION   DASOUD010000157 DASOUD010000000
VERSION     DASOUD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.161"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="U4A29_002546"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="U4A29_002546"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO0681638.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="U4A29_002547"
     CDS             complement(1172..1867)
                     /locus_tag="U4A29_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEO0681639.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="U4A29_002548"
     CDS             complement(1880..2596)
                     /locus_tag="U4A29_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEO0681640.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="U4A29_002549"
     CDS             2926..3534
                     /locus_tag="U4A29_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEO0681641.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
BASE COUNT         1062 a          764 c          662 g         1228 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttata
//
LOCUS       DASOUD010000158         3637 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.162, whole genome shotgun sequence.
ACCESSION   DASOUD010000158 DASOUD010000000
VERSION     DASOUD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.162"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..199)
                     /locus_tag="U4A29_002550"
     CDS             complement(<1..199)
                     /locus_tag="U4A29_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681642.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="U4A29_002551"
     CDS             complement(362..1996)
                     /locus_tag="U4A29_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0681643.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="U4A29_002552"
     CDS             complement(2000..3373)
                     /locus_tag="U4A29_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0681644.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
BASE COUNT          964 a          794 c          621 g         1258 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ctagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaatttt
//
LOCUS       DASOUD010000159         3492 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.163, whole genome shotgun sequence.
ACCESSION   DASOUD010000159 DASOUD010000000
VERSION     DASOUD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.163"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="U4A29_002553"
     CDS             complement(<1..2232)
                     /locus_tag="U4A29_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681645.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="U4A29_002554"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="U4A29_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          985 a          772 c          549 g         1186 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aa
//
LOCUS       DASOUD010000160         3468 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.164, whole genome shotgun sequence.
ACCESSION   DASOUD010000160 DASOUD010000000
VERSION     DASOUD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.164"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(688..1302)
                     /locus_tag="U4A29_002555"
     CDS             complement(688..1302)
                     /locus_tag="U4A29_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEO0681646.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1908..2285)
                     /locus_tag="U4A29_002556"
     CDS             complement(1908..2285)
                     /locus_tag="U4A29_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681647.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2585..3466)
                     /gene="galU"
                     /locus_tag="U4A29_002557"
     CDS             complement(2585..3466)
                     /gene="galU"
                     /locus_tag="U4A29_002557"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO0681648.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1053 a          693 c          513 g         1209 t
ORIGIN      
        1 cacatgaata atttattaga aaatcacttt tttctatttt gttttttttc tcctatgtat
       61 gattattttt ttctctttcc atttatatta agtattttat ttctctgttt ttccttaaaa
      121 ttcttttttt atttttagat tagagaaaat ctcgtcctgt ttttttcatt ttacaatcat
      181 tgaaaaattg aatttcattg caaaaaaaat aataaacact tataaatatc cattatctat
      241 ccttcaattt ttgtgtattt tatttctatt agaaaaaggt atcctgttgg acaattttgt
      301 tatatcaggt aaacttcatt gcaataatga taacaaatgt actataatta ctttgtaaag
      361 agttttgggg aagactcttt catcaacgtg tattaagaga caagaaagaa tccatatgga
      421 ttctttcttg tctctttttc cactactttt tatttttctg acactacata ttcttataac
      481 tcatttctca taactgtgaa acaacttata aaaataacaa actttaaaca aaacaagctt
      541 aaaatcaaag atgatccatc ttaaaataac taaaacaaaa aaagtagtcg ttgttaatcc
      601 tcattaataa tcgttttctt tttttcaaac gctccatttt tatgtggaac attatttttt
      661 taaggaactt tgtcaacatg tggataacta ttttccactt gtgtcctgga ctagctgttt
      721 tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg ctgttccgca
      781 gatcgctgga tctagatttg gcaaagtcaa gactgcatca tgtaaattgg cgtggttgtc
      841 caaaaccaaa tcagcaaatt caaataatcg caaaccactg ctatgttgcg atttgatact
      901 tcgtgacaaa tcaaatccag taacaatgat taatggatga ccattttctt tgatccactg
      961 agctagttcg atgattgccg ggtttcttcc ttcattcgaa agaagaagaa aaatgtcttc
     1021 tgatttgaca gaaagctgat caataattgc ctgtcctgca ccttctagcg tttcatagac
     1081 acctccggct ggattttcaa tcaatatact tggtacaaga tccggggttt gatcccaaaa
     1141 ttcaaatgct acaccactta gtgtgcggct agcatatatc tgcaatctac caccttgatt
     1201 aatttgtttc gcgatccaag tgccagcttg aagcatcagc ggctcttcta tccttaattt
     1261 ctcttccaat tggtgataat agtcaaaata acataaactc atttatctct cccgcctagc
     1321 tatacttgac atgaatagaa acagctgcct aatctaagga agtacataaa aaaacagtag
     1381 ttagacagta acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat tgaacgaata
     1441 cgcccaatac cttgacatag caaataaaac aacgtgcaga ccatggaaaa tatctacata
     1501 ggggtagaat tttcacccta gattaggtac aataaattag gtcgatgcac tgcattgact
     1561 atggcaaaat gatcggtcag ggtgtatgtg tgtgactggt cattccctga ttcttagcgc
     1621 caactaaggg tcagggagcc tttttttatt cagctgtttc tatctgaatt tattgtaagt
     1681 aatctcttat ccaagtgcaa gcaagaaaat acaaaaaaac attggtatga tttatatata
     1741 tataaataac ataaaaaaag gaatggctta tcccgataca acaagcattc ctttaaaaaa
     1801 gattctttta tataagtgaa aaaagctttt ttccaccaat taaataaatc tctcatctca
     1861 atagtttatc cattatttta tctttttttg cgtataccta ctaaatatta ccgtcttctt
     1921 ctcgttcttc tactttgttc gagagaatct gtactagaaa aagtagttgt atgtctacct
     1981 tttctgccta tccgagactt tcttttcata tatgggatat aaatgattag atacagtaca
     2041 gcaaaaaata aagcaaatac gcaagcttcc caactaccta acgccaattc aaccatcaag
     2101 gtagcaaccc caacggatgc tgcataccag ataaaaatca gaaacgtttc tttttttctg
     2161 accatgcgaa acatgataaa tagactaata agcaatagtg gcaacacaaa tatccaattg
     2221 cttccttcca tcagccactt gagtatatca agcagcgact gtgcaacagc gactaatttt
     2281 cccatagtga acacacacct ttttttataa ttaaaaaatt cgccttttta ttctactatg
     2341 aatcttctgg cttggctttc atttctctta tgtaatcatt ttcttttata caacttaaaa
     2401 aaaaattaaa ccattagcat aatattttct gaatctattc acgaaagtct ttttctatct
     2461 ttcgttttcc acgactcaac tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat
     2521 gacgcacaag ctgccgcttt aataacaaat agcctttcat tctcagcagg ccacttattt
     2581 ccagttaact ttctgttctt agctttttac tcaactggat aatatatgca cttaatgaag
     2641 tcccaatttc cgaatgttga agtccatatt caatatttgt cttcatatat cctaacttat
     2701 cgcctacgtc gtatcttttt cctttaaact ctctcgcata gaccgcctct gtctggttaa
     2761 gagtctgaag tgcatccgtt agctgtatct cgtttcctgc tcctggttct tgagtttcta
     2821 atatctcaaa tattttagga gtcagtagat aacgtccgat aatggctaaa ttacttggtg
     2881 cttttcccat ttcaggtttt tctacaaacc cttttactct gcaacgaccc ttttcgctat
     2941 ctgttttagg atcaataatt ccgtatttag atgtttcatt ttctggtaca cgcatgacgg
     3001 caacagcgga agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc aatgggatat
     3061 catctttcat caaatcatcc cccagcatga cgacaaacgg ttcatttcct ataaatgctt
     3121 ttgcttgaag cacggcatcc cccagtcctc ttggatgaga ttgacggatg aagtgaagat
     3181 tgacttgagt cgtttcttca actaaagcga gtaattctgt tttttccttt tcccgcaaat
     3241 tgttttccaa ttcgatattt gcatcaaagt gatcttcaat gggtcttttt tcttttcccg
     3301 ttacgatcaa gatgtcttcg attcccgaat ccaatgcttc ttccacaata aactggatcg
     3361 ttggtttatc cacgattgga agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac
     3421 gtgttcctag cccagcggca ggaatgactg cttttttgac cttcactt
//
LOCUS       DASOUD010000161         3398 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.165, whole genome shotgun sequence.
ACCESSION   DASOUD010000161 DASOUD010000000
VERSION     DASOUD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.165"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..276
                     /locus_tag="U4A29_002558"
     CDS             <1..276
                     /locus_tag="U4A29_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEO0681649.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="U4A29_002559"
     CDS             419..640
                     /locus_tag="U4A29_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0681650.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="U4A29_002560"
     CDS             656..1498
                     /locus_tag="U4A29_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681651.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="U4A29_002561"
     CDS             1561..3363
                     /locus_tag="U4A29_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEO0681652.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1229 a          469 c          702 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaata
//
LOCUS       DASOUD010000162         3396 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.166, whole genome shotgun sequence.
ACCESSION   DASOUD010000162 DASOUD010000000
VERSION     DASOUD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.166"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..871
                     /locus_tag="U4A29_002562"
     CDS             98..871
                     /locus_tag="U4A29_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO0681653.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMSNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1042..1408
                     /gene="ssrA"
                     /locus_tag="U4A29_002563"
     tmRNA           1042..1408
                     /gene="ssrA"
                     /locus_tag="U4A29_002563"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1628..1930
                     /locus_tag="U4A29_002564"
     CDS             1628..1930
                     /locus_tag="U4A29_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681654.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(2446..2519)
                     /locus_tag="U4A29_002565"
     tRNA            complement(2446..2519)
                     /locus_tag="U4A29_002565"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2483..2485),aa:Ile,seq:cat)
     gene            complement(3074..3388)
                     /locus_tag="U4A29_002566"
     CDS             complement(3074..3388)
                     /locus_tag="U4A29_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681655.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         1162 a          562 c          582 g         1090 t
ORIGIN      
        1 actgaaccaa tatgttcagg aattcttcac gaaaggtttg tcttttgttc attctttgtt
       61 aagataaact tattccaata aaaaaggagt tttatttgtg tcaaattatc gtgcaatcac
      121 tttctttgat cttgatggaa cattactaga tgcgaaatca aaaatcacac ccgaagtggc
      181 aaaagctatg aacgcattaa aagaaaacaa tgttctcccc gtcatcgcta caggacgtac
      241 agaagcagaa atccactcta tcatggaaga tgcgggcatt acttctgcgg tcaccatgaa
      301 tggttctttc attcgagtag acggcgaaga aatctattct caaacatttt cttcagaaga
      361 atgtcaaaaa atgtatgatc atgtacagtt gaacggtcat gaactttctt actataacga
      421 tcagaaaatc tggtgtaccg gtcatagcca aacaatgatc aatgcttacg acttcattca
      481 ttcagatgtt ccggaaattg atcctttagg ctttaaagaa aacacagtaa atatgttgct
      541 gatcttatct caatcaggtg acgaatacta tcacgaacat tttcctgagc taacttttta
      601 tcggaatggt ccttactcga tcgatacagt gaagaaagga atctcaaaag gggcaggtgt
      661 caaacgtctg caaaaagaac ttcagctgga aaaagttcct acttttggat ttggcgatgg
      721 tccgaatgat tttgccttgt tggaagcttg cgacaacaaa atcgctatga gcaacgccta
      781 tgacggatta aaagaacttt ctacctttat cacaaagaaa aatacagaag gcggaattgt
      841 ccacgcattg aagtattttg atctgattta agatcttttt ttgaagctac ctcttttctt
      901 aagaaaatcg cttatttttt cttaagaaag gggcagttat tatataaata atgggatatg
      961 attcgctttt ttattgaatt tatcccaata tatgctatac taattttgta tgaaaaatac
     1021 acgtaaccct tgttagctaa cggggacgtt acggattcga caggcacagt cgagcttgaa
     1081 ttgcgtttcg taggttacgt ctacgtaaaa acgttacagt taaatataac tgctaaaaac
     1141 gaaaacaact cttacgcttt agctgcctaa aaacagttag cgtagatcct ctcggcatcg
     1201 cccatgtgct cgagtaaggg tcctaacttt agtgggatac gtttcaactt tccgtctgta
     1261 agttgaaaaa gagaacatca gactagcgat acagaatgcc tgtcactcgg caagctgtaa
     1321 agtgaatcct taaatgagtt gactatgaac gtagattttt aagtggcgat gtgtttggac
     1381 gcgggttcga ctcccgccgt ctccattgtg agatttcagc agatttcgct agatgcttaa
     1441 atccttattt tataagggtt tgagcatttt ttattttcgc agattttcgt taaatgaatg
     1501 aaaatggcat tcaaaatagc attcaaaaag aacacttttt aatatatatg ttccatccat
     1561 attatcaatg atattcctac tttacaaaaa gaacattagt tcgtatactc tttttgaggt
     1621 gaacattatg ttgatggaag gaaaaacaca attatggttt aaatttgatc cttcgaatag
     1681 atttgtcaaa gatttttata aggtgtggga ttcagaagtt ttctttttag caatcgaaga
     1741 tagcttatta atcaatctct attactctaa taagaactac tttaaaattc cggcaacaaa
     1801 aaccagaatg aagaaagatg tgtatttttt gtttgatatc gtgactgatg tgccagacgc
     1861 acggagcgat catcggcgtt atgactattt gaagtatact ttcgttgatc cagaaaggta
     1921 taaagattaa agtaggctac ctaaaaaagg tagcccggaa cggattttat caccatactt
     1981 ataaaaggga tattttttaa gttagtatta agattgtgta atatgatgat atctatattt
     2041 tatagtatca gtgctataaa atcaaaaata agtcactaat taactaccac tccaattata
     2101 agtctttttc ccattatttt ttacagttat atggtatgct ttttaatggc ttcaaatata
     2161 aaagagttta aagcgtaaca cacttatggg gaagtggttt ttggggaacg ctttaaactc
     2221 ttctttatta ttatctcaca atataaccca aatgtctttc catttaaaaa tcaaagtaaa
     2281 acttttcaaa tatacagaag tataactatg tgaaacatcc tttcattaat ccataaaagg
     2341 atacataaaa aagccactca tttgagtggc aatgaagaaa agctttagct tgtataatac
     2401 tcttcaaaaa attctaacac agaacgattc aattggctac attaatgtac cctgtaggac
     2461 tcgaacctac gaccggacgg ttatgagccg tctgctctga ccaactgagc taagggtagt
     2521 ggttgttgcc acataaagcc ataaacaatc aaccagtaga atgcgtggca acaaacctgt
     2581 tatcgcatat cttggagtgt gactatttat gagtgatagt gaagatatgc gtctccatat
     2641 atgtatggtt gagacagata aacatgtatg aaaaacgttg ttaaatcaac gtttttttat
     2701 tttggtcaag gagagaaaag tagagaaata caaaattttt tgcattgatt ttgcattcaa
     2761 aaaaacattc aaacatttag aaatccccaa tacgtattca ggaaaatcta ttttaaggtt
     2821 tacaaaaaga acttttgttc gtacactctt tttgaggtga acttttatgc tgatggaagg
     2881 aaaaacacaa ttatggttta agcatgttcc ttcaaataag ttttttaatt ataaactcta
     2941 gagaaattaa ttcatcctaa cataaaataa ttgttcactc aaaagttttt ctagaacaat
     3001 taaaacagca ggcttcacat tcgctataca tttgtaagaa gtaataattc ctgtactaaa
     3061 aggcataatt gctttaatta ttttttgact tctcttcaaa cacaagaagt agttgtgtaa
     3121 taattgcaga aataaggatt ccaataatag atataaaatt tgacatggaa acgtctataa
     3181 cattaaaaga ataaagtgtt gaagaaagta aactgattga aataacccct ccgtaaaaaa
     3241 tagtctgtga atatacccac tttttttcag aagacgtact tcgtttggtt ctaaaaccta
     3301 tccctgcatt tctactctta tcaaaaaacc aaatatttag aagtaataat attgctatta
     3361 ttaagataaa attactgtaa aaatccaaaa gctatc
//
LOCUS       DASOUD010000163         3392 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.167, whole genome shotgun sequence.
ACCESSION   DASOUD010000163 DASOUD010000000
VERSION     DASOUD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.167"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..790
                     /locus_tag="U4A29_002567"
     CDS             <1..790
                     /locus_tag="U4A29_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO0681656.1"
                     /translation="YPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNTKVGDATIIRF
                     CQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLN
                     EAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVND
                     LVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGG
                     SLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            complement(1473..1571)
                     /locus_tag="U4A29_002568"
                     /pseudo
     CDS             complement(1473..1571)
                     /locus_tag="U4A29_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1836..2291)
                     /gene="tnpA"
                     /locus_tag="U4A29_002569"
     CDS             complement(1836..2291)
                     /gene="tnpA"
                     /locus_tag="U4A29_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEO0681657.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2534..3392
                     /locus_tag="U4A29_002570"
                     /pseudo
     CDS             2534..3392
                     /locus_tag="U4A29_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1121 a          585 c          590 g         1096 t
ORIGIN      
        1 ttatccattt ttaacgaagt ctgaaaaaaa agtcgctgat tttattttaa attctgggaa
       61 aactattatt tatagtacga tgagtgatat taagaaaaac acaaaagttg gcgatgctac
      121 tattattcgc ttttgtcaaa aaattggttt ttctggtttt tctgatttaa aaattgaaat
      181 tgcaaaggaa gactttagtc aaaaaaaaga acatccgact agtgtaaagt actatgatga
      241 aattgctaat agtttaacag aagctctcta ttccactatt aggcttttag acgaagaaaa
      301 attgaatgaa gccattaaaa tgatcactca atcaaaaaac atatatatat ttggagtggg
      361 atctagtgga aatactagtc ttgatttaga aaatatgttt cttcgcgtag gagttcaagc
      421 aaaagctgta ctagaccctc attttcaagc ccaagtcgct tctttattaa cagttaatga
      481 tttagtaatc attttttctt tatctgggaa aacgaaagat acttatgatt cattgaaaat
      541 agctaaaaaa aatggcgcaa aaattattgc tataactaac tatattcatt cacctatagg
      601 aaagtctgca gacttagttt tacaaacagc cattgaagaa tttcttaatg gtggatcttt
      661 agctggaaaa atatcacaac tttatatttg tgatctatta gttcatggtt atgaacaaaa
      721 caataatatt gattcagttg aattaagaga aaaagttttg cgctctatta ttgataaaag
      781 tattgagtga actaccccca cttagctaaa cctaacggtt tttaacgatt gaagtaggca
      841 attcttggga tgagagcata cttgatagct tattttttac taatagcggt ggctcttatt
      901 gaccaaatat ccctgtagtt cctacggttc ttgattttat ctaaagatat ttttatcgtg
      961 gaagctattg atcaacttga aagaaaaacg tgtagctcat tatcactagt ttatcgagta
     1021 tggatgaagc aatggatcat cctctcttaa ataaatttat taaggatctg atccttcaaa
     1081 ttttttccat gatcgatgag caggaaccta ctgaatctaa acgacatcgg gcacaaggaa
     1141 ttattaagat tgctaagcga atgttttcta taaagggtgt tctaagctat acagtgttaa
     1201 ttcaaaagat tcccaatcat cttgtttata agaacaacgt tgaagattaa aaaagtgttt
     1261 caatgctaaa attgcaaaat acttcaatgt tatgagacaa actgtctata agaatcaatt
     1321 tccacccatt tcatcacttg ttaaaagttt atgatccttt tgatttttat attaatttat
     1381 ctgtatttaa tacagatttt atttattagt tcatctgcct attgtatttt attttgttgc
     1441 cttaccatct gaagtaaata tactcttaat tgtcaaatta agttagacag atcatcaata
     1501 cttacgtaac tcaaaaatac ttccaaaggt gttcgataat ccagtgattt tctaggaaga
     1561 ttatttctgc gccagcattt tctaatgttt gaatttgtgt tttgtaatct tggttgcttg
     1621 tgctaattct tgaatagccg taaattgcca aaatttcatc accctttctg gacataagtt
     1681 tctgacacca gttaaaatca agtctatcag taacccatac acccgtgagg acgggtgtat
     1741 ttttaatccc cctagaaggg gtcgacactc gctgcccctt aaaaggggat ttttaaaaga
     1801 ccgccaactg ctcatcttca ctcacttact tctctttatt ttttaaatgg ttctacgtac
     1861 tctcttttac ttataatata tcgaatgcga tcatcagctt cttgttcacg gatgtatttt
     1921 tcatttgtct tttgattcaa tccgactgta ctcacatagt atctcttcga ccaaaaggat
     1981 ttgtttccgt atttatattt gagatttgcg tgctgctcgt ggattagtac cgcactacga
     2041 cctttcagat accccataaa actggatata catatttttg gtggtactcg aacaagcatg
     2101 tgaatatgat ctagcattgc atgtgcttct atgatttcca catctttcat ttcgcacaac
     2161 ttcctaagta tttttccaag atcttttcgt aattgcccat agattacttt tcgttgatat
     2221 tttggtgtaa aaataatatg atacttacaa ttccaacgag tatatgctaa acttttatcg
     2281 tcatttgaca tgaaaaaact cctttcgatc tgatgagcgg ttggtagcca ctcactagta
     2341 tatcgaaagg agtttttatc gctaaagata cttcttccac gcgtagaaac gcgcggtttt
     2401 cttgtttcgc acgctttacg cacaaaactg actgaagtca ttaagaaaat aaaagggtct
     2461 aaaactttta agttttgtac cgacccccaa aagttagacc aaaaaatcta acttttggag
     2521 gtcggtattt tttatggcta agtatacttt tgaacttaaa ttaaagatcg ttcatgatta
     2581 cttggatggt aaaggcggaa gtgattatct ggcaaagaaa tattccatta aggctccatc
     2641 acaagtgaag cgctggatca atgcctatca agaatttggt gaagagggtc tagtaagaaa
     2701 gcgacaaaag aaaaaatatt ctgttcaatt taagctggat gcgatagagt tatatctaac
     2761 aagtgagctg tcctatcgtg aagttgccaa cacacttaac atgaacaacc ctaatttgat
     2821 cgctagttgg atgcgtcaat tccgtgaggg tgggatcgat ggactctcaa aaacgaaagg
     2881 atgtccacct atcttgtcta agaaaaatga acccaaaaat aagaaaaaat ctacaactaa
     2941 agcaacgtct aaagaacgtg aacgcatcga ggagttggag aaacgagtcc gttcccttca
     3001 aattgaaaat gcctttttaa aagaattgag gaagctgcga aaacaggaag cccaacaacg
     3061 acgaatgaag caatcgcacg aatcatcgca agcctccgag gaccgtttaa attagttgag
     3121 cttctagaaa ctttaaagtt ccctaaggca atctttatgt actggcaaaa acgattggac
     3181 cgtaaaaatc cagatcaaga gattgaagcg gaaatgctta aagttcggga gaagcacaag
     3241 gattatggct gtttacggat gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa
     3301 aagaaagttc agcgcctgat caagaagcta ggaataaaag taacgacata cacacgtaaa
     3361 tcacggcgct acaattctta tcgaggacaa gt
//
LOCUS       DASOUD010000164         3221 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.168, whole genome shotgun sequence.
ACCESSION   DASOUD010000164 DASOUD010000000
VERSION     DASOUD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.168"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="U4A29_002571"
     CDS             301..585
                     /locus_tag="U4A29_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681658.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="U4A29_002572"
     CDS             588..1004
                     /locus_tag="U4A29_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681659.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="U4A29_002573"
     CDS             complement(1141..1860)
                     /locus_tag="U4A29_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEO0681660.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="U4A29_002574"
     CDS             2535..2630
                     /locus_tag="U4A29_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEO0681661.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="U4A29_002575"
     CDS             complement(2932..3177)
                     /locus_tag="U4A29_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681662.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DASOUD010000165         3218 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.169, whole genome shotgun sequence.
ACCESSION   DASOUD010000165 DASOUD010000000
VERSION     DASOUD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.169"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            158..847
                     /locus_tag="U4A29_002576"
     CDS             158..847
                     /locus_tag="U4A29_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO0681663.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            861..2363
                     /locus_tag="U4A29_002577"
     CDS             861..2363
                     /locus_tag="U4A29_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO0681664.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2376..2852
                     /locus_tag="U4A29_002578"
     CDS             2376..2852
                     /locus_tag="U4A29_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO0681665.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2949..3059)
                     /locus_tag="U4A29_002579"
                     /pseudo
     CDS             complement(2949..3059)
                     /locus_tag="U4A29_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1063 a          438 c          617 g         1100 t
ORIGIN      
        1 aactcctcta caatttcaac ttttctccat tatactccaa aataataaaa aaaactccta
       61 aatactccat ttttatcttt acttttatga aagcggatgc tatattgttt ttggagttgt
      121 tatccaaaaa taataaaaaa ttggagtgtt taggataatg gataaaaaga ggtttttaga
      181 tcttatttca aaaggtttga ttgtttcctg tcaagcatta cctggtgaac cattttacaa
      241 agaggaagaa agtttaatgc cttattttgc tttggctgct caaagggctg gagcagttgg
      301 tatacgtgca aattctgttc gagatattat tgaaattaga aagtgtgtat cattaccaat
      361 gattggaatt attaaaagag attatccacc agaatctcca ttcattactg ccactatgag
      421 agaagtagat gaattagtag aaaccggtgt tgaggtaatc gctcttgatt gcactcttag
      481 aaatcgacat gatggactat ctataaattc ttttattact aaaataaaag aaaaatatcc
      541 tcatcagtta tttatggcag atatttctac atttgaagaa ggcaaaaatg catataaagc
      601 aggaatagat tttgtaggaa ctactttaag tggatatact gaggaaagta aaaaacaaga
      661 tgggccagat atagatttga ttaaaaaatt ggtagaagaa gggatttgtg taattgctga
      721 gggaaggatt catactccag aacaagcaaa agagataaaa aaaataggag ttgcaggtat
      781 tgttgtgggt ggtgctatta ctcgaccaca ggaaattgca tcaagatttg ttaaagcatt
      841 aagttagggg gagaaataaa atgtttaaaa aattttcaca attaggacgc gctttcatgt
      901 taccaattgc tatattacca gtagccggat tactattggg actgggagga gcgttaacaa
      961 atgagagtgc tataaatgct tatccatttt tagatcaacc gtggttgcat acaattttaa
     1021 gtattatgag ttatgctgga aatgcagttt ttgcaaacct agcactaatt tttgcaattg
     1081 gtattgctgt aggacttgca aatggagata aaggaacagc tggtttagct ggtggagtag
     1141 cttatctagt atatactgca actattagtg ggtttcttga acttttttca gcaaaagatg
     1201 caacactaga tacaggtgta gtgggatcaa ttgttgttgg tggtactgtt gcatttttac
     1261 acaatcgtta tagaaaaatt gaattaccac aattcttagg tttttttgga ggatcaagat
     1321 ttattccgat tgtttctagt ttagctgcaa tcattattgg ttctttcttt tatttaattt
     1381 ggccaccaat tcaaaatggt ttagtagttg ctggtgaaca aattgcccaa atgggtagtt
     1441 taggaacttt tttatatggt tttttattgc gtttaacagg tgctgttgga ctacaccaca
     1501 ccatttaccc tttgttctgg tatacatctt taggtggtac cgaaactgta gctggatcaa
     1561 caattgctgg agcccaaaat attttctttg cccaactagc tgatccaaac cacactggtc
     1621 tgtttactta tggtactaga ttttttgcag gaagatttgc aacaatgatg tttggattac
     1681 cagcagcatg ttatgcaatg tatcgtgcaa ttcctaagaa aaatcgtaaa aagaatggtg
     1741 ggctttattt tagcggggcc cttacatcat ttttaactgg aattactgaa ccagttgaat
     1801 atatgttttt atttgttgct ccttggcttt atgtgattca tgcattctta gatggattat
     1861 ctttttattt tgctgatatc ttaaatattc gtgtagggaa ttccttttca ggtggtttga
     1921 tcgacttttt attattcggt gtacttcaag ggaatgacaa aactaattgg ttaaaattaa
     1981 ttccttttgg tattgcatgg gcatttatat atttctttgt ttttacattc tttatcaaaa
     2041 aatttaaggt agctattcct ggaatggaag aagaatcaga agttcattct gaaaaagtaa
     2101 taaacaaaaa tacatcatta catgaagagt ctcttatcat tattgaagct cttggtggaa
     2161 aagaaaatat tgagtctgtt gaagcttgtg caactcgttt gcgtgttgct gttacgaatg
     2221 gggagattat tcaaaaacaa acaataaaag acactggtgc aactgctgtc ttcgaagtga
     2281 agggtggcat ccaagctgtt tttggaggga aagctgactt attaagtcaa gaaatcaatc
     2341 aaatattagg aaaagataat taggaggttt ttaatatgtt tgaaattttc aaaaagaaaa
     2401 aacaagaaac tttttatgca ccttgttccg gaaaaatcat ttccatcaca gaagtaaatg
     2461 atcctgtctt ttcacaaaaa ttactaggtg atggttttgc cgtcatacct gatggttcaa
     2521 aaatttatgc tccattggct ggtcgagtaa caaatatatt tcctactaaa catgctatta
     2581 atataaaaag caactcaggc attgattatt tgattcatat tggaattgat acagttgaat
     2641 tacaaggaga gcctttttcg atttgtgttc aagaaaatga tgaggttact cctgaaacct
     2701 cgttggttga tgtggatttt gacaaaataa taaaatctgg taaagatact tctgttatag
     2761 ttgtttttcc ggagaaaaat cagttcaatg atttaataat agataatggt cgaattgctc
     2821 atgggaattc atgtggaaaa atcacttttt aaaaaagtgt atataaaaag ctataaaata
     2881 agctaaatag gatatttgtc aaatgaagtt ggtgaacaga aagaactgtt ttctttctgt
     2941 tcattttttt attttattag taatgattcc ccatgccaaa tcatgatccg taatttaaag
     3001 ttataaaagc ttcgaaatcc attggcattt cgttggaata ccttgatatg attattcaaa
     3061 tataaaatat agagagaaga gagagaggga agatatttat aaagggcaaa gttatgttta
     3121 ctcaacttag gaaatagaaa tagatcaatt tatattctct tgtcaatcga atatgaacct
     3181 tagcactata taccaaatat agtaaaatgc tgaaagat
//
LOCUS       DASOUD010000166         3168 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.170, whole genome shotgun sequence.
ACCESSION   DASOUD010000166 DASOUD010000000
VERSION     DASOUD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.170"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            135..410
                     /locus_tag="U4A29_002580"
     CDS             135..410
                     /locus_tag="U4A29_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO0681666.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            416..1183
                     /locus_tag="U4A29_002581"
     CDS             416..1183
                     /locus_tag="U4A29_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO0681667.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1351..>3168
                     /gene="typA"
                     /locus_tag="U4A29_002582"
     CDS             1351..>3168
                     /gene="typA"
                     /locus_tag="U4A29_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO0681668.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASK"
BASE COUNT         1098 a          538 c          674 g          858 t
ORIGIN      
        1 gaaaaatcag ttgcgttttg ttttttagaa ttatcaataa tattattatg taaacttaaa
       61 aatatgacaa cttcttggtt tattaccact tcctatgcta cactgaagag gaatatgaaa
      121 taggagagtt gaagatgcaa gaatatcagt atccacttga tttagattgg acaacagacg
      181 aaatggtcat tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa aaagggataa
      241 acaatgaagc ttttttgaag acttatcagc agtttaaaac tgttgtcaaa tccattggtg
      301 aggaaaaacg attgggacgg gaatttgaaa aagcttccgg ttattcttta tatcgtacag
      361 tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga gataaatgga
      421 acagatgatt gatcatatca agtcctggct tgaagaagct ggtgagaaaa tcaaatcttc
      481 tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa caaatgtcga
      541 tcgaaacatc cagaattttc taatcgaaaa aatccattcc tttgatccag aggcgaaaat
      601 attggcagaa gaagaagggt gtaatcaatt atcggatctc gatggaagag tctttatcat
      661 cgatcctatt gatggcacct tgaattttgt gattgaaggg gaaaattttt gtattatgct
      721 cgctgtttat gaatccggcg ttggaaagct tggtttcatt tatgacgtga tgagaaatga
      781 acttttttgg ggtggaaaag gaataggcgt gtataaaaat gatgagccat tgcctagacc
      841 agcaaataaa tccttatcgg aaggcttact tggtgtgaat agttatcttt tcggtcataa
      901 taaatatgga atccgagaaa ttgcggaaag cagcatgggg acacgtatgt acggatgcgc
      961 tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt gcttatattt caaaccttag
     1021 cccttgggat tatgcagcgg gaaatgtctt attggaagag tttggtatgg taacaagcgg
     1081 tctttttggt actcctttga catttgaaaa aagggaatac tatttagcag ggacttcttg
     1141 tgcatatgca gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag gtagaaatca
     1201 ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg aaaacatttt
     1261 actatatttc atgaaaaaag tctgctataa ttagcagtcg agtaaaattt gccctgtcat
     1321 aaagaaccaa taaaaaagga gttagttaca ttgaattata gaaacgatat ccgtaacgtc
     1381 gcaattattg cccacgttga ccacggaaaa acaacattag ttgatgaact attaaaacaa
     1441 tcagatacat tagacgcaca tacgcaattg caagaacgtg cgatggactc aaatgcgcta
     1501 gaaaaagaac gtggtatcac gattttagcc aaaaacacag ctgtagacta taaaggtatt
     1561 cgcgtaaaca tcatggatac tccaggacac gcggacttcg gtggcgaagt agaacggatc
     1621 atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac aatgcctcaa
     1681 acacgttttg tattgaaaaa agcactggaa caacatatca caccaatcgt tgtcgtaaac
     1741 aagattgaca aaccttctgc tcgtcctgag catgtcgtag atgaagttct tgaactattc
     1801 atcgaattag gagcagatga tgatcagtta gactttcctg tgatttatgc atctgcacta
     1861 aatggtacgt caagcttgtc agatgatcca gcagatcaag aaccaaccat ggcacctatt
     1921 tttgatacaa ttattgaaaa aatccctgct cctgtcgata acagcgacga accattacaa
     1981 ttccaagttt cattacttga ctacaacgat tatgtaggtc gtatcggaat cggacgtgta
     2041 tttagaggaa caatcaaagt aggagaccaa gtagcattaa ttaaattaga cggaacagtt
     2101 aagaaattcc gtgtaacgaa attatttggt ttctttggac taaaacgtct agagattcaa
     2161 gaagcaaaag ctggagactt gattgctgtc tcaggtatgg aagatatctt tgtcggtgaa
     2221 acagttactc ctgtggatca tcaagatgcg ttacctatcc ttcacattga cgaaccaaca
     2281 ttgcaaatga ctttcttagt taataattca ccatttgccg gtcgagaagg taaattcgta
     2341 accgctcgta agattgaaga acgtttgatg gcagaacttc aaacagatgt atccttacga
     2401 gttgaaccaa caaactctcc agatgcatgg acagtttctg gtcgtggaga attgcatttg
     2461 tctatcttga ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc tcgtccagaa
     2521 gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt gcaaatcgat
     2581 acaccagaag aatatatggg tagtgttatc gagtccttga gcttacgtaa aggtgaaatg
     2641 caagatatgg tccatacagg aaacggacaa atccgtttga cattcttaac acctgcccgc
     2701 ggtttgattg gttattcaac tgaattcctt tcaatgacac gtggttacgg aatcatgaac
     2761 catacttttg accaatatct gccaatgctt cctggtcaaa ttggcggacg tcaccaaggg
     2821 gcgcttgttt cgatcgatac aggaaaagca acgacttatt ctatcatgag tatcgaagaa
     2881 cgcggtacag tcttcgtaga accaggtaca gaagtatatg aaggaatgat tatcggggaa
     2941 aacagccgtg ataatgattt gactgtcaac atcacgaaag caaaacaaat gacaaacgta
     3001 cggtctgcta ctaaagacca aacatctgtc atcaaaaaac caaaacaatt gacgttggaa
     3061 gaatctttgg aattcttgaa tgatgatgaa tattgcgaag taacaccaga aagcattcgc
     3121 ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag catctaaa
//
LOCUS       DASOUD010000167         3150 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.171, whole genome shotgun sequence.
ACCESSION   DASOUD010000167 DASOUD010000000
VERSION     DASOUD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.171"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            36..827
                     /locus_tag="U4A29_002583"
     CDS             36..827
                     /locus_tag="U4A29_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO0681669.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            793..1746
                     /locus_tag="U4A29_002584"
     CDS             793..1746
                     /locus_tag="U4A29_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEO0681670.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2144..3118)
                     /locus_tag="U4A29_002585"
     CDS             complement(2144..3118)
                     /locus_tag="U4A29_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO0681671.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
BASE COUNT         1029 a          594 c          555 g          972 t
ORIGIN      
        1 caaaaattga cttttatgtt aaaggagtaa ttcatatgct gaaaaaagaa agacaagaac
       61 aaatcattac attattagag aaagattttt atttaactaa ttcagaaatc gctaacaaat
      121 taggcgtatc agaaatgacc atacgccgag atgtcactga attagcaaaa aaaaataaag
      181 taaaaaagct atatggtggc gttgaacgaa aggaacctta taatcgagaa ctatcgactc
      241 aagaaaagat tgatacgaat gtagccgaaa aaaaatatat tggtcaagtc atgaacagta
      301 tgattcccga taattctact atttatgtcg gtgctggaac aacgatccta tatgcacttc
      361 ctttcattca aaagaaaaat ctatttgtaa ttacaaacag tttgattgct ttcaactatt
      421 taaagaatca tacaagttat cggattttac ttactggtgg cgaattttct cctattacag
      481 aagagttcat tggggaagtt gcagaaagat cttttgaaag tttgaatgtc gatatctctt
      541 tcgctgcaac aaacggcata tttgataaca atgtgacaac cgctcaattt attgagggaa
      601 atattcagaa cgcagcgctc aatgcagcaa aaatcaaatg cattgttgca gattattcaa
      661 aattaaataa atcagacgtc tatactttta gaaaattaac cgattttgat tatctaataa
      721 ccgataatca tttatcagaa caggatttca atcattattc aaaatactta acaatattaa
      781 aggagcccaa aaatgatact gacaatcacg atgaacccat ccattgattc catttatttt
      841 acaaaacact ttgaattagg tacgatgaca cgctttgatt ctccggtacg ctatgtaggc
      901 ggcaagggaa tcaattgcgg acgagcttct gctctactag gttcatcagt tattttaaca
      961 gggtttttgg gcggttattt tggcagtatg attcaagata tgctgaaaaa tgaaaatttg
     1021 tttgatttgc attttttacc tattgcagaa gaatcaagag gtgccgtcac gatcatgcat
     1081 gatgatgaaa cgcagacaga gctagtggaa gctggtcctg aaatagcaga tactactatc
     1141 aaaaaattaa tgaatgatat caaactaatc agtaaaaata attacctttc agtcattaca
     1201 ataaatggtt ctgttaacaa tccagatccc tttttgtatg ctaaattatt acaatatatc
     1261 aaagaacaaa tagatccttc cacctatata ttaatggata tatctggaca acatctccgt
     1321 gaagtcttag caagtaagta taaaccgatg ttcatcaaac ctaatatcca tgagttttca
     1381 gatatcatcc atcaaaaaat tactcaaaaa agcgacataa aacaccatct aaaaacaaat
     1441 ccactattta aagatatcga tgttatcatg atttcttgcg gctcgtatgg tgcagtagtc
     1501 aaagtaaaag aagaattttg gaatgttgag atccctaaga ttcctatcat taatacaaca
     1561 ggctctgggg atgccactgt tggaggtttt gctcattcat tagaacagaa ttatccttta
     1621 aaagaatgtg taaggtacgc aatggcttgc ggcatgtcga atgcacagca atcagaagtc
     1681 gggaaagtta ttccagctga tgtaaaaaat ttagcaaaaa gcattaaaat cacagaaatg
     1741 aaataaatag tacaaagtaa ataattgttt atatgaaatg agacacaaag agactgtgac
     1801 aaaaaacgtg tgtaccgacc cccaaaagtt agattttttg gtctaacttt tgggagtcga
     1861 tacattgtca cagtcctttt ttcgaataaa cggtgttctc ctgccaactc ctcggtcaca
     1921 agtcacaata ttcaacaaac atgagaagct attttttgaa attgctcctt gtgactcaga
     1981 gttaaacggc aggctcacga cctcttggta taaacggtgt tcaaaagctt gcatctacgc
     2041 taatgagtcc aaccaagtca aaaatgagga gcttttttgg ctgtccttat tacttgatgc
     2101 agggcagttt tttcataatc tcttttagat taaatccttt tggttacaca aggttctctt
     2161 caaagaatgt tgccattttt tcaaatggaa tgatgtctgt ctggtcgtat agatctgtat
     2221 gggatgctcc tggaatgatc atgagttcct tgttgtcacc ttttagttct ttgaaagcgt
     2281 cctccccaaa gtaacgtgaa tgagcttttt ccccgtgaat gactaagact gcatgacgga
     2341 tttctggaat ataatgcaat agttttgcat tcataagtga caaccctgat gtcctattcc
     2401 aaccatcatt ggagtttaaa gaacggacat gatatccgcg gtctgtttta taatacgcat
     2461 gatagtcttt gacaaattga ggagcatctt caggcaacgg atcaaccaca cctccagcac
     2521 gtgcgtagtc cccttgtttg aaatcaatga tgcgttgtgc atttaattct tcacgcattt
     2581 catatcgggc attttcatcc ataacatcga agtagccatt tgctgttact cgagtcatgt
     2641 catacattgt ggaaacaact gttgctttga cccgcgtatc gatagctgct gcattcaatg
     2701 ccatacctcc ccatccacaa ataccaatga ttccgattct ctcagggttt acattttttt
     2761 gtacagataa aaagtctact gccgcttgga aatcttccgt attgatatca ggcgaagcaa
     2821 catatcgagg tactcctccg ctttctcctg taaacgatgg atcaaacgca atcgttaaaa
     2881 agcctctttc agccatcgtc tgcgcatata gaccagacga ttgttccttc actgcaccaa
     2941 atggcccgct cacagcgatt gccggcaatt tcttttcact tacttctttt ggttcatata
     3001 aatcagcgac taatgtgata ccaaaacgat tcgggaagct cacctttcga tgattgactt
     3061 gattgctttt tggaaatact ttgtcccatt cttgtgtgat gtttaattct ttattcatat
     3121 tctagactct cctttgatca aaattgacat
//
LOCUS       DASOUD010000168         3143 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.172, whole genome shotgun sequence.
ACCESSION   DASOUD010000168 DASOUD010000000
VERSION     DASOUD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.172"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(14..706)
                     /locus_tag="U4A29_002586"
     CDS             complement(14..706)
                     /locus_tag="U4A29_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO0681672.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="U4A29_002587"
     CDS             complement(1143..1712)
                     /locus_tag="U4A29_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEO0681673.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="U4A29_002588"
     CDS             complement(1786..2109)
                     /locus_tag="U4A29_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681674.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="U4A29_002589"
     CDS             complement(2263..3075)
                     /locus_tag="U4A29_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681675.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          870 a          632 c          552 g         1089 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaa
//
LOCUS       DASOUD010000169         3093 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.173, whole genome shotgun sequence.
ACCESSION   DASOUD010000169 DASOUD010000000
VERSION     DASOUD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.173"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(41..703)
                     /locus_tag="U4A29_002590"
     CDS             complement(41..703)
                     /locus_tag="U4A29_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO0681676.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            928..1212
                     /gene="groES"
                     /locus_tag="U4A29_002591"
     CDS             928..1212
                     /gene="groES"
                     /locus_tag="U4A29_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO0681677.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            1263..2888
                     /gene="groL"
                     /locus_tag="U4A29_002592"
     CDS             1263..2888
                     /gene="groL"
                     /locus_tag="U4A29_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO0681678.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         1070 a          530 c          687 g          806 t
ORIGIN      
        1 aatcaccaat ctaggatgac aggagtattt atagtcatgt ttatactagc cctttttgga
       61 cagctaattg aacaagaaca actaatgtgt tcattcctac gtgtgtgatc atcgatgtcc
      121 agatccgacc agttgctttg tactctaggc tgaagaagaa acctaggaaa aagtagatta
      181 gcagatgacc atcattatga gcaaacgcaa acagcaaaga gctgatcact gctccgaccc
      241 aaaaatttga atatcgactg atgatcccca gtatactgcg gcggaatacg aactcttcca
      301 tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg atcatctgga
      361 tgatattttg tgtattctcg gaaggggttg ctttgccaaa caatacagat tctaacatga
      421 cagcgatgct ttgcagaatc aatgcgacaa agattccaac caatccataa ataacaatct
      481 ttggagcaga agcttttttt accgctgatt cgatcgctaa gggttcacgt tgcttaaaat
      541 aaaaaatagc taggacaacg gcgcctagta tatacatgac tgcagttgct gtgatcaatt
      601 cagacgtaga ttttacaagt cctatcgaag ctaggatcaa gggtgagata aaaattagac
      661 catagcaaaa aatgctgagg aagctatatt tttttaaaga catgtaattc ctccttttaa
      721 gggataacaa gcatgcaagg caagcttata tcgattgatt tcaattatat cataatttca
      781 ataagcagct gtcagaataa aatttctttt ctttgattta aaagcttgca aaaaagaggg
      841 agaatgagta ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc taacagatta
      901 aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat taggtgatcg cgtcattatc
      961 gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc tgctgctaaa
     1021 gagaaacccc aaactgggac agttgttgca gtcggtgaag gccgtttgtt agaaaacggt
     1081 gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa atacgccggt
     1141 acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga tatcatggcg
     1201 attgttgagt aaatttggaa gataaaaaaa taatcaaaaa taatcaatga ggtgaatcaa
     1261 tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc agctatgtta cgcggggtag
     1321 ataaattagc agatacagtg aaagtaacat taggacctaa aggccgaaat gtcgtactag
     1381 aaaaatctta cggctctcca ttgatcacaa atgatggtgt gacgatcgca aaagaaatcg
     1441 aattagaaga tcattttgaa aacatgggtg caaaacttgt atcagaagta gcatcaaaaa
     1501 caaatgatat tgcaggagac ggtacaacga cagcaactgt tctgacacaa gcaatcgttc
     1561 gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct aggcattcgt cgcgggatcg
     1621 aattggcaac aaaagcagca gtcgaagaat tgcataatat ctcaactgtt gttgattcaa
     1681 aagaagccat tgcacaagta gcagctgttt cttcgggttc tgataaagtt ggacatttga
     1741 ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa gaatcaaaag
     1801 ggatcgaaac agaactagat gttgtagaag gtatgcagtt tgatcgtggt tacttatcac
     1861 aatatatggt aacagataac gacaaaatgg aagctgttct agaaaaccca tatatcttga
     1921 tcacagacaa aaaaatctct aacatccaag atattctgcc attgttagaa caaatcttgc
     1981 aacaatcacg tccattgttg attattgctg acgatgtaga tggtgaagca ttgccaactc
     2041 ttgtattgaa caaaatccgt ggaacattca acgtagtagc tgtaaaagct cctggtttcg
     2101 gcgatcgtcg taaagcaatg cttgaagata tcgctatttt gacaggtggt acagtaatta
     2161 ctgacgatct aggtttagaa ttgaaagatg taacaatcga aaacttagga aatgcttcta
     2221 aagttgttgt agataaagac aatacaacga tcgtagaagg ttctggagaa aaagaagcaa
     2281 tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga aacaacttct gattttgacc
     2341 gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt gttaaagttg
     2401 gtgcagctac tgaaacagaa ttgaaagaat taaaattacg cattgaagat gcattgaatg
     2461 ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct ctagtgaacg
     2521 ttatcagcaa agtttctgct gtagaagcag aaggagacgt ggcaacaggt atcaagatcg
     2581 ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga aaacgctggt tatgaaggat
     2641 cagtgattgt tgataaattg aagaacgtgg aactaggcac tggtttcaat gctgctactg
     2701 gagaatgggt aaacatggtt gaagcaggta tcgtagaccc aactaaagta acacgttctg
     2761 ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac gacagaagca gtcgttgcag
     2821 ataaaccaga accagcagcg ccagcagctc ctgcaatgga tccatcaatg atgggcggta
     2881 tgatgtaaga aaaagcaact tgcttatcta caactgatca ttagtgaaag acagataaaa
     2941 agaggctgga acagaagcgt ttaactccaa gaaataagaa gaaattcacg gaaattgctt
     3001 ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt ccgctgttta
     3061 tacgtgttta gagcgtgaga caaaagtgtt ctt
//
LOCUS       DASOUD010000170         3031 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.174, whole genome shotgun sequence.
ACCESSION   DASOUD010000170 DASOUD010000000
VERSION     DASOUD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.174"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            581..1540
                     /locus_tag="U4A29_002593"
     CDS             581..1540
                     /locus_tag="U4A29_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEO0681679.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1527..2132)
                     /locus_tag="U4A29_002594"
     CDS             complement(1527..2132)
                     /locus_tag="U4A29_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681680.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2372..>3031)
                     /locus_tag="U4A29_002595"
     CDS             complement(2372..>3031)
                     /locus_tag="U4A29_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO0681681.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          988 a          504 c          471 g         1068 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttaccttcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      541 aaaatagttc cgtcgaaaga attataagga gtgagtacaa atgacctata cccatcttac
      601 accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacactctg tttcaaaagt
      661 agcccatctg gtacaacgtt ctcgacaaac catttataat gtctatcgtt ttcttaaatc
      721 tggtggaagc gcaataagct actatgaaca atataagaaa aacaaacgta actgcggaag
      781 acgtccgatt gtgttaccag atgggcaaca agaatacatt caaaaaaagg tggctcaagg
      841 gtggactcca gatgtcctta ttggtcgagc agagtttcct attgcttgtt cggtacgtac
      901 actatatcgg atgtttaaag ataaaacctt tgaccctcac gatttaccaa tgaaagggaa
      961 aagaaaacca aacggacata aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca
     1021 ggatagaaca actgattata tacagttcaa tcatgagttt ggtcacctag aaggtgacac
     1081 cattgtcggt ggaaaacata aaagtgcggt gatcacgtta gttgagcgat tatctaaagt
     1141 gatcatcacc ttgaaacccg ctggtaggca agctatagat attgagaata gactgaatca
     1201 atggtttcaa tctgtaccga aacacttgtt taaatccatc acatttgatt gtggaaaaga
     1261 gttctcgaat tggaaaaaga ttagtaatac caatgatatt tcgatttatt tcgctgatcc
     1321 aggaacacct tctcaacgag gactaaatga acattcgaat ggcttattac gtaaagatgg
     1381 cttatataaa gctatggatt ttaataaggt atccgaaacc tttatccaat cggtggcatc
     1441 aaaacgaaat catattccta gaaaatcatt acactatcgc acaccattgg aagtattttt
     1501 gagttacgta ggtaaagatg atttgtctag cttaatttga caaacgaaac tttgtaaaaa
     1561 tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat gtcaatattt
     1621 ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa ttccctgtaa
     1681 ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc acccattcca
     1741 ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc agtttgttta
     1801 tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc aaaaattttt
     1861 tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac tgataattga
     1921 tactctatat tacatgactt aattagtttt ttagatcccc cctccagata ggcatatgtt
     1981 tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc ataaattaaa
     2041 aatgactgtc tatttagccc ataatctaat acaacttctt catactcata tttctttaaa
     2101 tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt gttatttatt
     2161 gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt agtgaactac
     2221 tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc ttgggaagag
     2281 agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac caagctatcc
     2341 ccgtagttcc tacggttctt gtttatttga tctaagccaa tcccatagag tgtagcctta
     2401 gaccttcggt caggatattg atactagcgt tgatatctcg gtcatgatga gtatgacaaa
     2461 tagggcaagt ccattttcga atttcgagag atttcttgcc gtctttatgt ccacaattcg
     2521 aacagatttg gctagatgga aaccacttat ctactttgat gattttccga ccgtaccagt
     2581 cagccttgta ttgtaattta gacacaaaac tagaccaaga aacatccgaa atactttttg
     2641 ctagtttatg attacgcaac atgccttttg tgtttaagtc ttcaatacag atgatatcgt
     2701 gatttttgat aatttctgta ctcaacttat tcagaaaatc agtacgttga ttcattactt
     2761 tttcatgcaa tcgtgctact ttacgttttt gtgtttgata atttctagct tcaaacagat
     2821 tgataccttt ttgtttagct aataaggcac gtcttgacaa cttacgttgt tcacgtttga
     2881 gtttcttttc cattttggac gtaaatttat tattatcaaa tttttgaccg gtagaaagaa
     2941 ttgcaaagtc tataatacct aggtcaatac caacagcaga attagattta ggtagttcgc
     3001 gaacttcttc tttgcataac aaagaaatat a
//
LOCUS       DASOUD010000171         2944 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.176, whole genome shotgun sequence.
ACCESSION   DASOUD010000171 DASOUD010000000
VERSION     DASOUD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.176"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            93..422
                     /locus_tag="U4A29_002596"
     CDS             93..422
                     /locus_tag="U4A29_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEO0681682.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..699
                     /locus_tag="U4A29_002597"
     CDS             412..699
                     /locus_tag="U4A29_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO0681683.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            983..1840
                     /locus_tag="U4A29_002598"
     CDS             983..1840
                     /locus_tag="U4A29_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEO0681684.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1841..2389
                     /locus_tag="U4A29_002599"
     CDS             1841..2389
                     /locus_tag="U4A29_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681685.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2649..2944
                     /locus_tag="U4A29_002600"
                     /pseudo
     CDS             2649..2944
                     /locus_tag="U4A29_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1114 a          377 c          592 g          861 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      661 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
      721 aaggacggct tctttttatc gaaaatctga ttcgtctaca tgactaaagt cgcgagtatt
      781 ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata aatcaatata
      841 tttcagcttt tccttattta tgtactacct tttacttatt aattataagg tagtacatat
      901 atatcttttt ttggtagtaa ctaaaaataa atatgttata atagtatcaa atgaatattg
      961 ctaaaagaaa agaggtagga acgtggtagc gaagaaatat gtaatcggta attttaaagg
     1021 tggcgtaggt aaaagtacgt gcgcgcaaat gttagggttt gaatcagcag ttaatgaagg
     1081 aaaaagaacc ttgataattg acttggatat gcaaggaaat acttctgatg tgatgaactt
     1141 aacgcatatg aacttcacta acgaagaagg aggcggagaa ggtgaaccga ttgtttacga
     1201 aaatactgtg actgacgtgt tgataaacaa tcttccacct aaagaggctg tctataaagt
     1261 aatcaataac ctctatatac ttccagcaga catgtccttt gaaatgtatg atgattggat
     1321 caaagaccaa tataatgatt cattatccca attcaaatat atgaaagaaa agctagaacc
     1381 attatttgat gagtttgacg tcatctattt agatgtccca ccttctattt ctgtatatag
     1441 taaatccgcg atgtacattg cggactgggc aatcgtcgtc ttacagaccc aagtaaaatc
     1501 gatgagaaat gcgatgcaat atcttgagta tatggacttt tttgtgaaag aatttgatac
     1561 aaatttatat gttgctggtg ttattccttt catgttggaa agcggtgacg cagtagatca
     1621 ggaaatgtat aaacaagccc aagagattta tggggaacat ttgattaaaa atgttgtact
     1681 caaaaatgcg cgcttaaaac gatacgacgg atcaggtatt acgatggaga agacaaagaa
     1741 aggtctctta aaacaatggg atcgcaaaag ccacgaatta tttatcagta ttttgaaaga
     1801 attggaagaa cacgagaatt ggtatgcgga ggtggagtaa ttggctgaca tcaaaggact
     1861 tgatattttt gggaacaaca aaggactcgc caaaataaaa aaagaagcag aagcaaagaa
     1921 aaagcagcaa gcaattacac catctgaaaa taagcaatta gaagtgcacc aagttcaaga
     1981 aataaaaact tcctccaatg ttgaaagaca gagtagagga agaacaaaaa aagttggcgc
     2041 accaatcaga aagtttgata aggtttattc caccaagcat cctttaaaac tgtcgacttt
     2101 gttaaatgcg acttccagag ttttagtaga aaagtatgag actaatatga caagagatga
     2161 attgcttaga aaagctttag atgagtatat taaacagaat cttacgcaag aagataagca
     2221 agatttgttt aatgacgtgg tgaaagaact agacttgttc agagaaaaat atccaattgt
     2281 tccagaatta gatgatcgtg gagaaataat aagaactacc agtcagataa aacaagatac
     2341 cgaagactca ctgcgcaaag ggtggggaat gaataataaa gttagataat aatcaaagaa
     2401 gaactaagta actaccttta aacggtagtt acttagttct tctttgattt gcatgaaata
     2461 gaggggattt atttttctgg ttacgtgatt aaaaaatcca aaaaatgagc ataggaacgg
     2521 ttatgatttt agggtgtgtt ttaatgagat attgtgattt cattaacaag tgtatgataa
     2581 tctgttgttg agttagtaaa taagtaatag agctaactct aaaataaaac tattaggagg
     2641 agatgtaggt gaaaaagttt aaaaagaaaa tatatttagg attggttgtt ggggtgagta
     2701 tgtttgggtt catggctcat actaaacaag taactgcaga tactaaccaa aaagaaagga
     2761 ttatgagcca attagatgac tctcaaataa gtatgtaccc aatgagctaa ttgtcagttt
     2821 aaaggctaca caaagaattt caacagagaa aaataaaccg caaaatttat tgggtgtaaa
     2881 agttatggtg gaaaaagatt tgactcttag gacaaatgaa agcgattgta ataagcgaga
     2941 tcaa
//
LOCUS       DASOUD010000172         2600 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.177, whole genome shotgun sequence.
ACCESSION   DASOUD010000172 DASOUD010000000
VERSION     DASOUD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.177"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..295
                     /locus_tag="U4A29_002601"
     CDS             83..295
                     /locus_tag="U4A29_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681686.1"
                     /translation="MIFQGVITLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIIL
                     SFLVNAIVLFLYVLNYLDINPLIFSR"
     gene            459..713
                     /locus_tag="U4A29_002602"
     CDS             459..713
                     /locus_tag="U4A29_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681687.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            783..1028
                     /locus_tag="U4A29_002603"
     CDS             783..1028
                     /locus_tag="U4A29_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0681688.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1104..1469
                     /locus_tag="U4A29_002604"
     CDS             1104..1469
                     /locus_tag="U4A29_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681689.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1530..2093)
                     /locus_tag="U4A29_002605"
     CDS             complement(1530..2093)
                     /locus_tag="U4A29_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681690.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          765 a          468 c          413 g          954 t
ORIGIN      
        1 tgcgatttta ttcgatttag ttgcggcagc agcttatttt ctccaattaa attatcaaac
       61 ctcaggaatt ctgtttattg gaatgatttt tcaaggtgtg attacccttt tgcttcttgt
      121 catgatgctc agctataaag gaaaaagata tgcccgtatt caaacaaaag tatttgttaa
      181 atatgtttcc attcgctatg ggatcatcat tctttccttt ttagtgaatg cgatcgtcct
      241 ctttttatat gtattgaatt acttagatat caatccattg attttttctc gatagataag
      301 acacattcag cggaacagtt cctgcttatc agcagaaaac tgttctgctt tttcattttt
      361 cattgatcta ttcttctttt attttcctag catttcattt gtgatgtatg caataacatg
      421 ttatctttta ttatatgaat cgttgaggag tccgttacat gattacgttg gtgagagttt
      481 tgttttgggt gcctgcggtc gcactggtcg caagtattgt ttatcttatg aattggaaca
      541 aagaacggtt ttatttagct attttgactc ttccggctat ctactttatg tggaaagttt
      601 ttaattacaa ctactttgaa cccgatagtg tttttataga agaattatca ggactggttt
      661 tgtctttatt gatcgtcatt ctttatttga tacgtttaaa taaaaaacat tgagtactat
      721 tgattgcatg tgtagctttc tatagtcatt ataactattc tactataatg aaaggtgatt
      781 aaatgagtat cgaagaagct attttactgg atactttacg ttgtcgtctg aatttcttaa
      841 gaactggccg atctttatct caagtagctg tttctagcgc gttaagtatt cctttattca
      901 aatatcgtaa gtacgaagaa ggagaagaaa ttccaagcat cattgaatta atctatatcg
      961 ctgactttta cgatatttcc gttgattacc taattggtcg ctcagaaaca gcaagcaaac
     1021 tgaagtgatt ttttccagcc ttggtgctgg ttttttcatt taacgtctaa ggttaacaat
     1081 ctatatataa aggagacatt tctatgcgat tatggcatga aaaattgatt tcaaagcttc
     1141 ctcgtcaaca gcttttagga caacatcggg aatgctgtgc attaagagga aatggctggg
     1201 gaaagaaaca tgctactgtg gattatgttt ttcactattc tccttataga ttattccaat
     1261 atcatcagct tgtcatggat gaaatggaaa aacgtggtta cagcccagca ccagagtgga
     1321 aaaatcctat gtatcgtggc aaatcatgcg aagcattttt tgatttacct actgtagagc
     1381 gcaacgtgcc gatttatccg gaacacgatg aagtttatct agcagaatgt ttagcaaatt
     1441 tggagcaaaa aggaatttat cttacctaat ataactagaa aagccttcta tctcctctta
     1501 cttcattaaa aagctttaac catcttagat caagctaaaa tgtcgtaagt tttagggtta
     1561 tcttcatcct cacgtgcaaa actaaaagcg tactctagtt ttggttcatc gccaacttcc
     1621 ctagacataa atgaacatct cctgacgtgg tttaaaagta ccgcatcttc gacgtctgta
     1681 tacgatgcac agcacagatt gcttcttttg atctttcttt tgatttttct gttgcttctt
     1741 ctacaccttc ttcgattact acatcgatgc ttacaattgc ttctgcctaa tattcccatg
     1801 catcgtcgat ttaagcctct gtagtcactg tgatcgtttc cacattttaa ctcctctgtc
     1861 gtggcttcag ttgattttgt gatcagttca gttgctaagc cagagtcaga acaacctgcc
     1921 attgctagcg ttaatattga tagtaaaaat agttttttta tcataaggtt ccccccctga
     1981 aaatagacta aaataataac gctttctttt tttgttttca gacgaaaatt aataatttct
     2041 aaatattctt taaaaaagct tataactatt atgccttttt taatatataa cacagttatt
     2101 tttaaaaata agaatgctaa atcactctac aataacggcg aaagcacaaa aaataaaaaa
     2161 attattacgt tattaaaaaa acagatgaaa gatcaggttt tattagatct cattctttca
     2221 tcacttgaag ggatttaatg aactactttt tcctctttgt cgtttttata tattcgtatt
     2281 ccttcaaaca gtaggatagc ctctttcaat gcttgatatt tgaactgtcc gttacttaaa
     2341 aatttagatg tgccgccctc ttcctagaac atattctttt ttactttttc attaattgac
     2401 agaccaatcg aacgaaactg taaatttcga taggataatg attggtcaaa ctcattcatt
     2461 ttcaagacat ctcgattcat attcaagata ggaatctttt tatcaacatc actttattcg
     2521 tattttcctc taccataaaa gcttcacgct ccacaaaatt gttttataca ctactattca
     2581 gcccattcaa cgaccaatga
//
LOCUS       DASOUD010000173         2594 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.178, whole genome shotgun sequence.
ACCESSION   DASOUD010000173 DASOUD010000000
VERSION     DASOUD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.178"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1152
                     /locus_tag="U4A29_002606"
     CDS             <1..1152
                     /locus_tag="U4A29_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEO0681691.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="U4A29_002607"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="U4A29_002607"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO0681692.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT          859 a          491 c          621 g          623 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actg
//
LOCUS       DASOUD010000174         2411 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.180, whole genome shotgun sequence.
ACCESSION   DASOUD010000174 DASOUD010000000
VERSION     DASOUD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.180"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..2276
                     /locus_tag="U4A29_002608"
     CDS             123..2276
                     /locus_tag="U4A29_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO0681693.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
BASE COUNT          822 a          425 c          533 g          631 t
ORIGIN      
        1 caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct
       61 tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg
      121 aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa
      181 aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa
      241 attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt
      301 tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg
      361 tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga
      421 ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc
      481 ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag
      541 atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa
      601 gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata
      661 aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta
      721 aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg
      781 atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca
      841 cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga
      901 cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt
      961 ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag
     1021 gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag
     1081 aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat
     1141 caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac
     1201 agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa
     1261 tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga
     1321 ttactagcgc ctttgctcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg
     1381 caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg
     1441 cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag
     1501 cattggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag
     1561 actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat
     1621 tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg
     1681 aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca
     1741 aagaatggta tactccaggt aactttaccg atttggcatt tggacagttt gacacatata
     1801 caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc
     1861 cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga
     1921 aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc
     1981 tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacagca gccaaaccat
     2041 tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag
     2101 gtcatccaga tattacgaca gtcaatagta atatcgtggc atacggacca acagacaatc
     2161 cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac cacatgaact
     2221 tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa
     2281 gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc
     2341 tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact
     2401 cggcaaaaaa g
//
LOCUS       DASOUD010000175         2398 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.181, whole genome shotgun sequence.
ACCESSION   DASOUD010000175 DASOUD010000000
VERSION     DASOUD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.181"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="U4A29_002609"
     CDS             207..2069
                     /locus_tag="U4A29_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO0681694.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="U4A29_002610"
     CDS             2069..>2398
                     /locus_tag="U4A29_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO0681695.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          465 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attaaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DASOUD010000176         2388 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.182, whole genome shotgun sequence.
ACCESSION   DASOUD010000176 DASOUD010000000
VERSION     DASOUD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.182"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(366..1658)
                     /locus_tag="U4A29_002611"
     CDS             complement(366..1658)
                     /locus_tag="U4A29_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEO0681696.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1932..2192)
                     /locus_tag="U4A29_002612"
     CDS             complement(1932..2192)
                     /locus_tag="U4A29_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681697.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          805 a          445 c          364 g          774 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggattaatgg ttacttacac
     1741 ggtttatatt acactctcat cattaaaaaa gtatctttcg atattactaa acaatgaaat
     1801 tatctatttg aaatttaaat ctgataatat attgagtgtt ccaatgaggg ctttgatcct
     1861 attatccata tctttttcag ttttaatatt gatacacatt ataagaaaaa gaaacttttg
     1921 gttaatttat ttcatcccag tgcaaaatat aaaaaagtaa aatagaccat aggcaataga
     1981 ttaacaatta atataataca agcaaatatt ctattcattg tatttctgct ttcaaaaagt
     2041 tctaatattg aaagtataat tccacaaatg cctataataa gtatgggaat aagattagaa
     2101 aataacaccc cgagccaatc tgtgaaaatt actatattta ctaatatccc tattaacata
     2161 cctatcatat ttattaaatc aatttttttc attagatatt tctcctttat taaatctgaa
     2221 gtacttatat taaaataatt ctaaactatt tacctcaaaa agtgcaataa attatttctg
     2281 aacaataaaa tgatagtcaa aacaaaaaaa tattgttaat cgttcagagc catccgagag
     2341 tgtaaaatat tttgtgtaaa tgaaaaaatc catacaaaaa aggaaggc
//
LOCUS       DASOUD010000177         2295 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.185, whole genome shotgun sequence.
ACCESSION   DASOUD010000177 DASOUD010000000
VERSION     DASOUD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.185"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1015..1305)
                     /locus_tag="U4A29_002613"
     CDS             complement(1015..1305)
                     /locus_tag="U4A29_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681698.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2205)
                     /locus_tag="U4A29_002614"
     CDS             complement(1513..2205)
                     /locus_tag="U4A29_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO0681699.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          656 a          434 c          335 g          870 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgagttt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg taatt
//
LOCUS       DASOUD010000178         2259 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.186, whole genome shotgun sequence.
ACCESSION   DASOUD010000178 DASOUD010000000
VERSION     DASOUD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.186"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..421
                     /locus_tag="U4A29_002615"
     CDS             <1..421
                     /locus_tag="U4A29_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0681700.1"
                     /translation="NNISLVFQNYNLIDYLSPIENIRLVNKSADESILFELGLDKKQI
                     KRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNK
                     CVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
     gene            421..1797
                     /locus_tag="U4A29_002616"
     CDS             421..1797
                     /locus_tag="U4A29_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0681701.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1927..>2259
                     /locus_tag="U4A29_002617"
     CDS             1927..>2259
                     /locus_tag="U4A29_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEO0681702.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          884 a          276 c          406 g          693 t
ORIGIN      
        1 aaataatata tctttagtat ttcaaaacta taatttaata gattatttat caccaattga
       61 aaatattaga ttagtaaata aatcagcaga tgaaagtatc ttgtttgaac taggtttaga
      121 taaaaaacaa ataaaaagaa atgttatgaa attatctggt ggacagcaac aaagagtagc
      181 tattgctaga gcattggtat cagatgctcc aataatacta gctgatgagc ctactggtaa
      241 tctagacagt gttactgctg gagaaataat taatatatta aagacattag ctaaagatag
      301 aaataaatgt gtaatagttg taacacatag taaggaagta gcagattctg cggatatcat
      361 tttagaacta agtggtaaaa agttgaaaaa agtaaataaa atgaatttgg aggttgaata
      421 atgataaaaa atgcatttgc ttatgtaact agaaaaagtt taaaatcatt aattatcata
      481 ttagttattt tatctatgtc aactttaagt ctcattagtt tatctattaa agatgctacg
      541 gacagagctt caaaagaaac atttgctaat ataacaaata gtttttctat ggagataaat
      601 agacaagtaa atccaggaac acctagaggt gggggaaatg taaaaggtga agatattaaa
      661 aagatatctc aaacagatag tatagactcc tatgtaaaaa gaataaacag tgttgcagat
      721 ttagttgatt atgatattat tgaaactcaa gagactcttg cgaatcaatc acctgaaaga
      781 gcaaagaatt ttaaaagaac agttatgtta actggggtta acgactcaac aaaagaaaca
      841 aaatttgtat cagaagcata taaattagta gaaggaaagc atttagaaaa tgaagataaa
      901 aataaaatct taatgcataa agatttagct gaaaaaaata accttaaagt tggagataag
      961 ataaaaataa aatctaattt atttgatgct gataatgaga aaggtgcaga tgaaacagta
     1021 gaagtagaaa ttaaaggtct attcgatggg cataatagcg gtggagtaag tgcagcacaa
     1081 gaactatacg aaaatacatt aatcactgat attcatacag ctgctaaagt ttatggtaat
     1141 accgaagata cagctgtata ccaagatgca acattctttg taaaaggtga taagaatctt
     1201 gatagtgtaa taaaagatct tgggaaatta gatataaatt ggagagaata taatttgatt
     1261 aaaagttcat caaattaccc tgcattacaa caatctatat caggtattta ctcaatttca
     1321 aataaattat tttttggctc attaatattt gcaggagttg tagtttcatt attattattc
     1381 ttatggatga atgctagaaa gaaagaaata gcagtattac tatcattagg tatatcaaaa
     1441 ttagaaattt ttggtcaatt cctaattgag atggtattta tatcaatccc cgcattcgtt
     1501 ggttcctatt tcttagctca atatacagct gacaagcttg ggaataatat attgaataag
     1561 gttactggtg atatagctaa acaaatagct aaacaatctg catctagcca attaggtggt
     1621 ggggcagaag ctgaaggatt taacaagaca ttatcaagtt tagatatcaa tgtattacct
     1681 aaattcatga tttatgtagt tatatttatg agtttagtac ttcttgtatc attgattatt
     1741 tcttcattca atatattaag aaggaatcca aaagaattat taattgacaa taattaaaat
     1801 tttcggtgct ttttagcacc aaaaatgaga gtgtaatata aaccgtgtaa gtaaccatca
     1861 atcctaggcg ggtggttgct tcacggtttt tatttttcta aaataagatc aagaggtgat
     1921 tacacgatgg ctagaaagaa aagaaatcct gatgctgaga agttagctga atccattttg
     1981 aatacttatc aacccgaatc tgtagaggat atgcaagatg ttttaaaaga tgtgtttggt
     2041 cccttatttg aaaaaatgct tcaaggagaa ttgaataacc atttagggta tgacgcccat
     2101 tctaaagagc caaagaaaca tgacaatcga cgaaacggtt atggcaataa gacgcttaaa
     2161 acgagctttg gtaaagtaaa cattgatgtt cccagagacc gggaggcttc ttttgaacct
     2221 gagttgattc ctaatagaaa acgagatgtc tccgacatc
//
LOCUS       DASOUD010000179         2255 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.187, whole genome shotgun sequence.
ACCESSION   DASOUD010000179 DASOUD010000000
VERSION     DASOUD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.187"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(284..1624)
                     /gene="glnA"
                     /locus_tag="U4A29_002618"
     CDS             complement(284..1624)
                     /gene="glnA"
                     /locus_tag="U4A29_002618"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO0681703.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1652..2044)
                     /locus_tag="U4A29_002619"
     CDS             complement(1652..2044)
                     /locus_tag="U4A29_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO0681704.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          642 a          499 c          381 g          733 t
ORIGIN      
        1 cgtctttttc aaaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagt
       61 tcagccaatt caaaaacaag aaaagctgtt tttgcttggc tattcttatt acttgttgca
      121 gggcagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta
      181 gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt agtttctcta atactcaggt
      241 caaaacgctg atttcacagc ctctaattca cggtattcct gttttaataa agttctagat
      301 attgttctct ttcccactcg gaaacagttt gtctgaatgc cgcccattcg agacgttttg
      361 cttcaacaaa atttgagaag atatgttgtc ctaatgcgtc aatcattaca ttatccttac
      421 gcaattcttt gatagcatta tggatagttg aaggtaagtc ctggatatgt gcagcttctc
      481 tttcttcctc atccattaca taaatgttac ggtcaacagc tgctggcggt gtgatctgat
      541 tacggatacc gtctagtcct gcttgcaaca atactgccat tgccaagtat gggtttgcag
      601 atggatcaac tgaccgcaat tccaaacgtg tggataaacc gcgagattct ggtacacgta
      661 ctaatggcga acgattacgg ccactccatg cgacataaac aggcgcttca taacctggaa
      721 cgagacgttt ataagaattg actgttggat tgcaaacggc tgtgtaggca cgagcatgtt
      781 tcagcaaacc acctaagaaa tgataagctg tttcacttag ttccaattca ccgtctttat
      841 catagaaaac atttccttca tcattgaaca aagacatatt gcaatgcata ccagaaccat
      901 tgatcccaaa taacggtttc ggcatgaaag tcgcgtgaag tccatgcttg cgtgcaatcg
      961 ttttgacaac gagcttgaag gtttggatat tatcacaagc atctaccaca tctgcatatt
     1021 taaagtcaat ttcatgctgt cctggcgcta cttcatgatg tgaagcttcc acttcaaagc
     1081 ctagactttc taattccaac acgatgtcac ggcggcaatt ttcacctaaa tctgttggtg
     1141 caaaatcaaa ataaccacct ttatcattca aggtcgttgt gatcccaccg tcttcgtcta
     1201 atttaaataa gaagaattct ggttcaggtc ctaaattgaa agaggtaaaa ccaaggtcgc
     1261 gcatatcttt caatgctcgt ttcaaattcc cgcgaggatc gccggcaaat ggtgtcccat
     1321 ccggattata gatatcacaa attaaacgag caacttttcc atgagcactt tcccacggga
     1381 agatcatcca agtggagaga tctggataca aatacatatc gctttcttca attcgaacaa
     1441 atccttcaat cgaagaaccg tcaaacatca ttttattttc taatactttg tcgatctggc
     1501 tcactggaac ttctacattt ttgatcgtac ctaagatgtc tgtgaacatc aatcgtaaaa
     1561 atcgtacgtt ttcttcctca atgattcgtt tgatttcttc acctgttatc tgtttcttta
     1621 ccatggtatc cctcttcctt ttttaattta ttcagtcaaa gaattgtctt tgcaatcctt
     1681 gcgactgata atgacctgtt ttgcttaacc caccttgcgc caaaatctca tcacgaaaaa
     1741 tttgtcgaac gtcatgatct gtcaacggct tcttgttttt tgcttcttgt tttttcgcct
     1801 gtgccatttc gtatacacgt ttgatcccag ccatattcaa tccctctgaa agatagtctt
     1861 taatctcaag caacatatca atatcattca gcgaatacat acgtcggttt ccttcgcttc
     1921 gttccggatg aactaaatct tgttcttcat aatagcgaat ttgccgagca gacaaatcag
     1981 tcagcttcat aactgtacca attggaaata cggacataga tcggcgcaat tccttttctc
     2041 ccatcagacg tcccctttcg ttttataagt tggctaaagt gtaccaatta aaaaaaatat
     2101 tgtcaactgc atatgttagg ttttctaaca tgagatctct attttttata aaataacaaa
     2161 aaaataagaa cacaaaaaag gtatctagtt gattttcgta ccagatacct tttatttttt
     2221 attatactgt actatccttt attagtgttt ttatt
//
LOCUS       DASOUD010000180         2228 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.188, whole genome shotgun sequence.
ACCESSION   DASOUD010000180 DASOUD010000000
VERSION     DASOUD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.188"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..280)
                     /locus_tag="U4A29_002620"
     CDS             complement(<1..280)
                     /locus_tag="U4A29_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEO0681705.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="U4A29_002621"
                     /pseudo
     CDS             742..2124
                     /locus_tag="U4A29_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          655 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 tgtaaaaaac tattgcacaa tacaagtagc ctacggctat cgaaatttcc ataactttcg
     2221 agcaagaa
//
LOCUS       DASOUD010000181         2189 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.189, whole genome shotgun sequence.
ACCESSION   DASOUD010000181 DASOUD010000000
VERSION     DASOUD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.189"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(325..528)
                     /locus_tag="U4A29_002622"
     CDS             complement(325..528)
                     /locus_tag="U4A29_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681706.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="U4A29_002623"
     CDS             complement(987..1952)
                     /locus_tag="U4A29_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO0681707.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DASOUD010000182         2139 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.190, whole genome shotgun sequence.
ACCESSION   DASOUD010000182 DASOUD010000000
VERSION     DASOUD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.190"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            136..309
                     /locus_tag="U4A29_002624"
     CDS             136..309
                     /locus_tag="U4A29_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681708.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="U4A29_002625"
     CDS             949..1422
                     /locus_tag="U4A29_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEO0681709.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="U4A29_002626"
     CDS             1431..1658
                     /locus_tag="U4A29_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0681710.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          694 a          342 c          317 g          786 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgat
//
LOCUS       DASOUD010000183         2103 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.191, whole genome shotgun sequence.
ACCESSION   DASOUD010000183 DASOUD010000000
VERSION     DASOUD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.191"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(118..708)
                     /locus_tag="U4A29_002627"
     CDS             complement(118..708)
                     /locus_tag="U4A29_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEO0681711.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="U4A29_002628"
     CDS             1231..1392
                     /locus_tag="U4A29_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEO0681712.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="U4A29_002629"
     CDS             1411..1905
                     /locus_tag="U4A29_002629"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEO0681713.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1898..2059
                     /locus_tag="U4A29_002630"
     CDS             1898..2059
                     /locus_tag="U4A29_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681714.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          753 a          330 c          360 g          660 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taa
//
LOCUS       DASOUD010000184         2003 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.192, whole genome shotgun sequence.
ACCESSION   DASOUD010000184 DASOUD010000000
VERSION     DASOUD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.192"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="U4A29_002631"
     CDS             <1..378
                     /locus_tag="U4A29_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEO0681715.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="U4A29_002632"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="U4A29_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEO0681716.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="U4A29_002633"
     CDS             1859..>2003
                     /locus_tag="U4A29_002633"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681717.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DASOUD010000185         1932 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.193, whole genome shotgun sequence.
ACCESSION   DASOUD010000185 DASOUD010000000
VERSION     DASOUD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.193"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            202..1167
                     /locus_tag="U4A29_002634"
     CDS             202..1167
                     /locus_tag="U4A29_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO0681718.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQ
                     LRRGKATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYK
                     YRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
     gene            1367..1570
                     /locus_tag="U4A29_002635"
     CDS             1367..1570
                     /locus_tag="U4A29_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681719.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINSLSGRK"
BASE COUNT          640 a          288 c          436 g          568 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta atcacactag
       61 ctccgtaact tcttaatcat tatacgaata tttttagatt tcttcaagac tagagatttt
      121 ttggtaatga atttttaaga gttacgtgaa cttttgaaaa ttctttccag aagtctcttt
      181 ttctttgagg aggaacaaaa tttgagtaaa aatggcataa tcgtaaatga gtgggaaaat
      241 ttaacagccc tatctaacag gcggttaaat gttcaaaatt tgaacacccc cctaaaatta
      301 cagtggtcaa ttgaccgaat tactatcgtt ggaaaactga aggaaaatat ttattatcac
      361 actcaaaatg atgtgttgat tttagatttt gagcaattaa tgcgggtgaa tgaaggcaat
      421 ggctatttaa aagcggttgg gaataacggc tggcaattat tagaccaata cgaagaaaat
      481 attgcttata ttgagatttt gaaatggcaa gatggcaaag ggcggattga ttttaacccg
      541 aataagattg gtcatttttt agctagttca atgaaaaatt tcattcatga tttatttcta
      601 gaaccacatt tctcacgtgc ggatattgct tgcgatatta tcgatgtgcc tgatgagttt
      661 attacacaat atcgagtggt tgatcctgtt tctttcaagc caatttatgg gcggtctggg
      721 aagctggaaa cagcttattg gggcagtcgt gccagcgaac gacaaattcg gctatacaat
      781 aaaaaattgg aacaagaaac caaacgaaaa gttgtaccaa aggaagttga gacttggtgg
      841 cggttagaaa tgcaactacg gcgtggcaag gcaaccgatt ggcataatat ggttcaagaa
      901 agtttgaata gttttgcgag cctgcatttt ttgccgattg atacaacacc tacagataga
      961 ataatgctcg ctggtttaat gtctgatcaa aatttctggt ctcttttgtc gaggaattca
     1021 aaatataaat atcgggaatt gctgaaagaa gaaagtcaaa atgatgaatt gacgaatcat
     1081 ttgcgagaaa cgtttgcaga atcggcagac aatttgaaac aagaattaga cacgtggctt
     1141 ttgggtttgg acgtgacaga gaagtgatac cgaataagaa aagtcagatg agtgagagta
     1201 aatttacgtc aatgtttaat gacgtgagtt tacgataagc gattattttg cgtgaagcaa
     1261 aataaagcgt aaaaggagtt gagagcgtga cacgctttcg gctccttttt tcgttgtcac
     1321 ttgtgacagc gaataccctc ggagagcatt tggaattgtg acagccatgt cacaattagg
     1381 aaaatgttac tctttgtcaa agagtaacag aaaggtttac actcttttta ttagcgacag
     1441 cgcgaaggag gggtttaact tggggtttag tatttcgttc aaaaagggaa caaaaaatac
     1501 aagcatttct cataataatc gtgagttaac tgatagccaa aaaattaata gtttgtctgg
     1561 tagaaaataa ttagcagatt tacaagtggg aaataaaaag ctgaaagaag aaaattgcat
     1621 ttcaaactac attttctaca ataactctaa aatggcatat tttgcgttct gagcggtttt
     1681 agcgtttatt tcgtttagtt atcggcacaa tcggtgaaac acgttagagg agcgaaaatg
     1741 gagcctgtaa ggcgactggt agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa
     1801 cgaaataaat gctggcagcg ccattctatt tcggcaggag gaggctcaag gggttcgggg
     1861 aatgaaattc cctgaggtct ttttattttt tctagaggag tgctataatc aaagaaaatg
     1921 agaaaaaaga gt
//
LOCUS       DASOUD010000186         1861 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.194, whole genome shotgun sequence.
ACCESSION   DASOUD010000186 DASOUD010000000
VERSION     DASOUD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.194"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..85)
                     /locus_tag="U4A29_002636"
     CDS             complement(<1..85)
                     /locus_tag="U4A29_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO0681720.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            155..316
                     /locus_tag="U4A29_002637"
     CDS             155..316
                     /locus_tag="U4A29_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEO0681721.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="U4A29_002638"
     CDS             335..1612
                     /locus_tag="U4A29_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO0681722.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..>1861
                     /locus_tag="U4A29_002639"
     CDS             1622..>1861
                     /locus_tag="U4A29_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO0681723.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKN"
BASE COUNT          748 a          179 c          321 g          613 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 t
//
LOCUS       DASOUD010000187         1765 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.195, whole genome shotgun sequence.
ACCESSION   DASOUD010000187 DASOUD010000000
VERSION     DASOUD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.195"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..236)
                     /locus_tag="U4A29_002640"
     CDS             complement(<1..236)
                     /locus_tag="U4A29_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0681724.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHR"
     gene            378..938
                     /locus_tag="U4A29_002641"
     CDS             378..938
                     /locus_tag="U4A29_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO0681725.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(1258..1557)
                     /locus_tag="U4A29_002642"
                     /pseudo
     CDS             complement(1258..1557)
                     /locus_tag="U4A29_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          577 a          322 c          245 g          621 t
ORIGIN      
        1 cgatgtttct ttttttctcc ttttatctcc gccagcaaaa cacctgccaa ctcgtcctct
       61 gcatacaaag caagaatttg agttgctcga ttcaactcca tatacaaaaa atatcttccg
      121 taaagattca gcagaagttc attttccata tatctctcaa agttcattcc ttcaacagca
      181 aaatagatag cttttttgta atctttcttt tctaagggtt taaaagttag tttcatcatt
      241 tcgcctcctc aacttcaatg attgtgttat acttacatta taatagacac agtgtctact
      301 atctataatc aatcatctat aagtttgtcg attaagcgac agctttgctt tgatatgtcg
      361 gataaggaga taggtaaatg aaaaagacag atagacgcgt caaaaaaaca gaaaaagctt
      421 tggctgaaac actatccaca ttattagtaa ataaaaagat tcaagcaatc actatacgcg
      481 aactaacaga aacggcagat gtacatcgtt cgacttttta tacacattat aaggatatct
      541 atgatctata tgatcaatta gaaagcaact tttttcggga tctaaatgaa attctctctt
      601 atgatccagc ccatagctat gaggaactat acactcgctt gattgactat gtttacgcaa
      661 atactttttt atatcgctta tttatcctca accatggtga tatttcgttt cagaagaaag
      721 ttaaacaagt tattgaagaa aactatctga acatatggct atatgaagac aagaaaacga
      781 ttatcacaga agaaatgaga ttctttacta cgtaccacgt ccaaggttgt ctatccgttc
      841 tcaatcggtg ggtagaaagc aattacaagt actccaaaaa agaattatta gaactcttac
      901 agaaactgaa tgatcacata gagaaaatca tgccttgaat aaaaacatac taagcttagt
      961 taagaagaac cgtaaccttc tcgtataaca actgaatctt atttagccaa attgtttact
     1021 gaagattttt tgagtaaaat ttctataatt cctacttatt gaaaagtagt ttgtagtgct
     1081 gcttctccct ctctaagtga tatatcttgt tgtaccaatc aaattcacct catcattaat
     1141 aaattacttt taggctaatt tattacaact tataggttga agctgtaacc aatttatagt
     1201 catattatat ttttatcatc cagtttattg aattcttctc gtttacctca attcatttta
     1261 aaggggaatc agctcatcat ctaacatttt acgccatgct cgcttcattt cttgtgggta
     1321 tttaaatggt ttcgcttctg gatgtttggc tttatatcct tcgtaatcct tactgttgat
     1381 aattgctttc atattaggaa ttggatcttc acctggcata tattgcagat tagaagcatg
     1441 taattttttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat catcacttac
     1501 ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca ttgctatcta gtgtaccttc
     1561 atttctttgt gcttaaattc aaagataatt ttctcaataa agatttttga ttccagtaag
     1621 attcctttac tccttttaca acatagcgtt gtcctttgtt ttgaaacatc ttcttcccct
     1681 ctttgccatt aaaaatagcc caattctcta taaagaagat taagcttcat tattataata
     1741 taaagtttaa atttggttta ttact
//
LOCUS       DASOUD010000188         1695 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.196, whole genome shotgun sequence.
ACCESSION   DASOUD010000188 DASOUD010000000
VERSION     DASOUD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.196"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(18..1484)
                     /locus_tag="U4A29_002643"
     CDS             complement(18..1484)
                     /locus_tag="U4A29_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO0681726.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          539 a          332 c          280 g          544 t
ORIGIN      
        1 taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat ttctttgttt
       61 ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac aattggtgtg
      121 atgatattcg tgaataattg gaaccaccat gctttaggat cagcagtata atctactttt
      181 ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc accaaagata
      241 aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt ataagcttta
      301 ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac aactgagttc
      361 aagttcgtca accattttac taattcattc atatctttga taccaaagat cggcaacaaa
      421 ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt acctttacga
      481 ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa caagattttt
      541 agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt tgtcaatgcg
      601 taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg aaaagcactg
      661 tatgccccat ttgccatcaa gtctttagga atattatcgc tagcaaacag catacccatt
      721 gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat catcccttta
      781 ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt ttccgctcct
      841 ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa tgttggaata
      901 tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt gatgaacggt
      961 gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt tccggccagt
     1021 ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa ggcaagaaat
     1081 ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat atttccattt
     1141 ccttggactg cccaaccgcc agcaatcagg attgcttgag gcttttgtgc caaataagga
     1201 atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt agatgtcgta
     1261 ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgaccc taattgtcca
     1321 acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga ggtgacaact
     1381 gatactcctt gctgagcata gttattaact acattcccca tgccccaaac catgttaaat
     1441 gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc tctcctttat
     1501 gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt acaagacagt
     1561 gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat tttttccatc
     1621 aaaaaaataa gttattttct tttatttcat ttaccttatt gaatttcaag ctattttgaa
     1681 ttttctgcaa aatta
//
LOCUS       DASOUD010000189         1670 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.197, whole genome shotgun sequence.
ACCESSION   DASOUD010000189 DASOUD010000000
VERSION     DASOUD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.197"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            502..834
                     /locus_tag="U4A29_002644"
     CDS             502..834
                     /locus_tag="U4A29_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEO0681727.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1453..>1670
                     /locus_tag="U4A29_002645"
     CDS             1453..>1670
                     /locus_tag="U4A29_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEO0681728.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
BASE COUNT          572 a          240 c          354 g          504 t
ORIGIN      
        1 aaatttagtt gttttaaatt taattgtctg ctacgcttgt gatgaattaa ttttcgttct
       61 gcacttttgg acgataaatt tggacaattg aaaatacaaa aaaaagaaag gccctgtgag
      121 actcggaagg ctggtaacct tcgtcgacgt ccacaggtgg cattaagaca acctttaatc
      181 ccttaatgcg tttacctttc agtgttacgg tattacccgc aacggattta gtataacaga
      241 tattcaaaag tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg
      301 atgaatctat gcctaagcat aatgttataa aaatgtccgt gtggctcttt gccgtatgac
      361 gaacgctgaa attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg
      421 aacgaaaatc aattctctat ttttgaaaaa taacaagaaa aacttattaa aaatatttaa
      481 agtaagtggg tggattattt tatgtcatat acaggaattt tgagtctcga agatatttgt
      541 cactacggta aacggtgtac cgctacagag aaaatcacga agaaactatc aactggccaa
      601 agtaaaaccg ttgtgcagtg taagaaatat ataatccaga aagacaaagt ctcagaggaa
      661 atgatttatt acgctggtaa acaaaagcaa ataattttaa aagatcctat ccctttaaaa
      721 gaactatatc caacaatcaa acatgtttat gaccaaaacg gtgtactgat tggtcgaaga
      781 aaaaatggag tcttgcgttg taccgcaaaa gggatggggc gtttaatcag ctgaattgat
      841 cattctcata acttgtattt tatcgttttt cgcttgcttg aaaatattgt cgagacttct
      901 aaaagaactt agaattacta gaaaaataag gagtagactt gatctatcaa caattccttt
      961 cggggggtga cggggtaggt ttttacgatt ggtaacgtaa aaaattggtc aatcgagtaa
     1021 atgcccttgt ttggatagtg gtgaaatgaa tttgtgtaaa taatatgcac agagtaaaca
     1081 agagggaatt tagaagttag acaagttaga atagcgccta gattaatcat gagcagctac
     1141 taagggatct aggctttgac gcaaactcat ggaataagga gtaggtcttc tcgtaatggg
     1201 gaggacgcac aagaaattgt gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt
     1261 ggtcattgga tcaatcgagc atttaccgat ttctactagg gtttctcgtt tcggacgcca
     1321 taaacgagtc gatatttttt atgtattttt aagtaaacga aaaacgataa aatatgaacg
     1381 agtcctgaat aaagtgaaaa aattgatata aaacatagag aaaatgaaat ttacgtaatg
     1441 gaggaatgac aaatgaaaaa ttatgatccg aatatacgat tgggcacaca tactattaag
     1501 gtttctttcc aacgttggga ttacaaagga tttgtgacgt ttagaagagg cggaaattgt
     1561 aaaggattag atgttttagc attagacgag gatgatttat actatcaaaa attgactgat
     1621 aatccaattg gattcggctt gctacctgaa gatgatgaag gtaatgaatg
//
LOCUS       DASOUD010000190         1575 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.198, whole genome shotgun sequence.
ACCESSION   DASOUD010000190 DASOUD010000000
VERSION     DASOUD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.198"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..1292
                     /locus_tag="U4A29_002646"
     CDS             123..1292
                     /locus_tag="U4A29_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEO0681729.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          559 a          255 c          258 g          503 t
ORIGIN      
        1 ttttacactc tctattatga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacatataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1381 ataattgagt tttattaaac tttttaagtt tctattctat caaatattta aacaatctct
     1441 gcatccgaaa agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa
     1501 tcctgatttc ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt
     1561 ccatggataa taata
//
LOCUS       DASOUD010000191         1561 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.199, whole genome shotgun sequence.
ACCESSION   DASOUD010000191 DASOUD010000000
VERSION     DASOUD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.199"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            333..740
                     /locus_tag="U4A29_002647"
     CDS             333..740
                     /locus_tag="U4A29_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681730.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            754..1155
                     /locus_tag="U4A29_002648"
     CDS             754..1155
                     /locus_tag="U4A29_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681731.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1157..1528
                     /locus_tag="U4A29_002649"
     CDS             1157..1528
                     /locus_tag="U4A29_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681732.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          585 a          192 c          271 g          513 t
ORIGIN      
        1 cttttaatta gatttctagt ctaacttttg gggtgcacat catttttgag gggaagtaca
       61 tatagttgtt gatatagttg ctaatattgt tagctaattt gttagtaaaa ctgtatacga
      121 atttagatag taaaaaaaca gtttgttcct tatatagagg cacttttact actgattaaa
      181 tgtcagatat gcttacaaat atacttataa atatgcttac aaaattagct aatatttccg
      241 ttgacaaagc tttgttacta ttgttaaact aaattgacta gcaaaagtga aacataaatt
      301 tattaaataa atgtaaaaat taggagaaat caatgtctta tagagtacaa ttaataattt
      361 cagaagatgt agaaagtcag caatttggca ctaatgttat taataaagta attaatccag
      421 ctctttctat taatgcgcct ttaattccta ctgctttatc attttctgta acagctgtgg
      481 tgtctgagat agaggataca gaaaagataa aaatagtaga aattgaagtt ttaaacaaga
      541 atgaaaaaca aattttttct acgggtgaag tatcggtaag cttgcctcct caagttaacg
      601 atataaactt taatataaac gccagaaatg tcttggttga agaagcagga gagcattatg
      661 ctgttgttaa atttaatgga actgagattg gaaggcaaat ctttgatatc aaggtcaaca
      721 aaccagtgga aaaaaattaa ggagacgata cggatgcttg atatagtacc atcaaataca
      781 catcggagtg gtagcttaat aaattttgct gcgatttttg cttgcgcatc ggtaattcta
      841 gcatctccag ttgttgtgga tgcaagttct actccgaaaa ctaaaaatga taatcaactt
      901 gtaattacta caaaaaatga gattaacaca atttcaagta atgatggcaa aatttttgat
      961 gtgataaata cggttttaaa aagaaaagct gaaatcaata taaaatatga tgaagattta
     1021 aacttgtatt tctttgttat caaaacaaca tctgaattgt tttcctcaga ttacgatgtt
     1081 ttggatactc tcgataatgt attatctgac tataaatata tggggaaatc tgtagtagca
     1141 actttggggg aataaaatgt ttgactttaa tgaatattta tctatttcca aacaaattga
     1201 atcagatgat aaatataatt ctaaagaatc ttatagaaga actgctgttt caagagcata
     1261 ctacagtgct tttaaaaaat ctgatgagta tttgaaagaa aattatgata ttatttataa
     1321 tggaagtagc ggaaaaggaa gccaccaaac cgtttggaat ttgttttcaa cagttaagga
     1381 attgaatacc ttaggaatac ataatagtgg gtatagaatg ttagagaaaa gaaaatgtgc
     1441 tgactatatt cctaatgaaa ccattacgaa aacagatatg gcgctgatga atcgagaagc
     1501 agaaaaaata ataaacaaac tcacctaatg aatttcaagc gagtttgttt gttatttttt
     1561 t
//
LOCUS       DASOUD010000192         1490 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.200, whole genome shotgun sequence.
ACCESSION   DASOUD010000192 DASOUD010000000
VERSION     DASOUD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.200"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..475
                     /locus_tag="U4A29_002650"
     CDS             137..475
                     /locus_tag="U4A29_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681733.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="U4A29_002651"
     CDS             complement(680..1255)
                     /locus_tag="U4A29_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEO0681734.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          461 a          310 c          251 g          468 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
//
LOCUS       DASOUD010000193         1456 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.201, whole genome shotgun sequence.
ACCESSION   DASOUD010000193 DASOUD010000000
VERSION     DASOUD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.201"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(100..1119)
                     /locus_tag="U4A29_002652"
     CDS             complement(100..1119)
                     /locus_tag="U4A29_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEO0681735.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1130..1336)
                     /locus_tag="U4A29_002653"
     CDS             complement(1130..1336)
                     /locus_tag="U4A29_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO0681736.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          403 a          303 c          246 g          504 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg
     1141 atgaaatttt cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa
     1201 aacctgtaat aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa
     1261 gaccgacaaa agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa
     1321 aggttctatt gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat
     1381 cgcttgtttt cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc
     1441 agttgttcaa ttttat
//
LOCUS       DASOUD010000194         1455 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.202, whole genome shotgun sequence.
ACCESSION   DASOUD010000194 DASOUD010000000
VERSION     DASOUD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.202"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..1040
                     /locus_tag="U4A29_002654"
     CDS             108..1040
                     /locus_tag="U4A29_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEO0681737.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1455
                     /locus_tag="U4A29_002655"
     CDS             1112..>1455
                     /locus_tag="U4A29_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681738.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          227 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caaga
//
LOCUS       DASOUD010000195         1418 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.203, whole genome shotgun sequence.
ACCESSION   DASOUD010000195 DASOUD010000000
VERSION     DASOUD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.203"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="U4A29_002656"
     CDS             complement(150..1337)
                     /locus_tag="U4A29_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEO0681739.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttca
//
LOCUS       DASOUD010000196         1409 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.204, whole genome shotgun sequence.
ACCESSION   DASOUD010000196 DASOUD010000000
VERSION     DASOUD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.204"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1359
                     /locus_tag="U4A29_002657"
     CDS             <1..1359
                     /locus_tag="U4A29_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEO0681740.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DASOUD010000197         1382 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.205, whole genome shotgun sequence.
ACCESSION   DASOUD010000197 DASOUD010000000
VERSION     DASOUD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.205"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(175..1316)
                     /locus_tag="U4A29_002658"
     CDS             complement(join(175..1088,1088..1316))
                     /locus_tag="U4A29_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO0681741.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          344 a          278 c          288 g          472 t
ORIGIN      
        1 gatttgagtc aatacctcgg acacgatttg tgagaatttt ttcgagattc cagcgggtga
       61 gtatctcatc gtttcgacca gcggtcaaac caaaaccgtt tgaccgctga tcgaagcgat
      121 gaagttgaaa ccctgtggaa atctcgccaa aaaaatgtaa gtggtagcta ggaactaagc
      181 ttgcaatgcc aactcctcca tctgatttgg tgttaaatag ttgatactac tatggattcg
      241 cttgcgattg taaaatcctt caatgtattg aaataacgct aaataagcag cttcaaagtt
      301 ttcatacgct tttgatggat agacttcttc ttttttgagt gaggcgtgaa acgattcgat
      361 accggcatta tcgtagggac agcccttacg actgaaggag tgccgaatgt tcaattcatt
      421 cagctttgcc tcataggctt cgctagtgta ctgagaaccc aaatctgagt ggatgatcaa
      481 gttctcggag ggtgtttggt ttaagagcgc attgtcaagg gtttcgataa ccaattcttt
      541 cgtcatctgc ttgccaaact tccaaccgat gactttcttt gtgtggaggt cttgaatagt
      601 ggagaggtag gtccagccat cttttagggt atgaatatag gtaatatctg tcacccattt
      661 ttgattgatc gacgtagtgg taaaatcttg gtttaacagg tttttacgtc cttcactttt
      721 aactttcgct tgtttgccag gcttgtactt cttgagggtg actgaacgca agcctaattt
      781 tctcatcagt atttgtactt tctttaagga caaggtctcg ccacggccaa caagaatctt
      841 atgaatttta ggcgcaccat agataccctt gttttcaaag tagattcctg aaatcaagcg
      901 gtttaagcgt ttgttttctg attggcgctt ggaaggtttc tttttcttcc agtcatagta
      961 gctggatttc ggtgcttcca aaacggtaca catttgtttt acgtcatgat catgaacatt
     1021 tgattgaata aattcgaaga catcactcat ttttactttt gagcgaatat ggttaaagcc
     1081 ttttttagga tagtgttttc ctcctgcatg cgggccattt cctttttcat cgctaggatt
     1141 tctgcctcag tcatgccggt tgctacattc tttgtatgaa gcttcttcca acgataaatc
     1201 gtttgtgtag cgataccata ttgcgctgcc agttcttcga ccgattggcc agactggttt
     1261 aaatctaaga tcatcttctt aaagtcttca tcaaattttt tcttggcttt tgccatcgtg
     1321 gacacgtcct tttcttagta gttagattct accaaatcgt gtccatgaaa tcatcctaac
     1381 at
//
LOCUS       DASOUD010000198         1377 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.206, whole genome shotgun sequence.
ACCESSION   DASOUD010000198 DASOUD010000000
VERSION     DASOUD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.206"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..392
                     /locus_tag="U4A29_002659"
     CDS             <1..392
                     /locus_tag="U4A29_002659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO0681742.1"
                     /translation="RYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIED
                     LPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVA
                     YLVNNEYIFKTKFSTNKKKGDKVRIIV"
     gene            470..>1377
                     /locus_tag="U4A29_002660"
     CDS             470..>1377
                     /locus_tag="U4A29_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HEO0681743.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYI"
BASE COUNT          530 a          213 c          247 g          387 t
ORIGIN      
        1 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
       61 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      121 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
      181 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
      241 agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata
      301 gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga
      361 aaaaaggtga taaagtccgt ataattgtgt aaaagtaaaa aggccatata acagtccttt
      421 tacggtacaa tgtttttaac gacaaaaaca tacccaggag gacttttaca tgacccaagt
      481 acattttaca ctgaaaagcg aagagattca aagcattatt gaatattctg taaaggatga
      541 cgtttctaaa aatattttaa caacggtatt taatcaacta atggaaaatc aacgaacaga
      601 atatattcaa gcaaaagaat atgaacgaac agaaaaccga caaagtcaac gaaatggcta
      661 ttatgagcgc agctttacga cacgtgtagg cacgctagaa ttaaaagtac ccagaacacg
      721 tgatggccat ttttcaccca cagtgtttga acgttatcaa cgaaacgaaa aagccctcat
      781 ggcttcaatg ttggaaatgt atgtatcagg cgtttcaact cgtaaagtat caaaaattgt
      841 ggaagaactt tgtggtaaat ccgtctctaa gtccttcgtt tctagcttaa cagaacagct
      901 agaacctatg gttaacgagt ggcagaatcg tttattatca gaaaaaaatt atccttactt
      961 aatgaccgat gtactctata taaaagtacg agaagaaaat cgagtactct caaaaagctg
     1021 tcatatagcg attggaataa ccaaagatgg cgaccgtgaa attatcggct tcatgattca
     1081 aagtggcgaa agcgaagaga cctggacaac attttttgaa tacctaaaag aacgcggttt
     1141 acaaggtacg gaactcgtta tttctgatgc gcacaaagga ttagtctctg ccattagaaa
     1201 atccttcacc aacgtaagtt ggcaaagatg ccaagttcac ttcctaagaa atatctttac
     1261 caccattcct aaaaaaaatt caaaatcttt cagagaagct gttaaaggaa tttttaagtt
     1321 cacagatatt aacttagcgc gtgaggctaa aaatcgattg attcatgatt atatcga
//
LOCUS       DASOUD010000199         1375 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.207, whole genome shotgun sequence.
ACCESSION   DASOUD010000199 DASOUD010000000
VERSION     DASOUD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.207"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1342
                     /locus_tag="U4A29_002661"
     rRNA            <1..1342
                     /locus_tag="U4A29_002661"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          379 a          298 c          412 g          286 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aactt
//
LOCUS       DASOUD010000200         1338 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.208, whole genome shotgun sequence.
ACCESSION   DASOUD010000200 DASOUD010000000
VERSION     DASOUD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.208"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..457
                     /gene="spx"
                     /locus_tag="U4A29_002662"
     CDS             47..457
                     /gene="spx"
                     /locus_tag="U4A29_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO0681744.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            471..827
                     /locus_tag="U4A29_002663"
     CDS             471..827
                     /locus_tag="U4A29_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEO0681745.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          490 a          195 c          250 g          403 t
ORIGIN      
        1 aagaaattac tgtagaattg gattataatt aaaaatgagt gagaaaatga taaaagtata
       61 tacttcgcct agctgtactt cctgtagaaa agcaagagca tggttgcaag cgaatcaact
      121 tgaatttgaa gagaagaata tttttgccga accgttaaca gaaagtgaga tcaaaaagat
      181 cttggcagcg actgaaggag gagttcaaga tattatttcc actcggtcga aaatttacga
      241 aaagctgggc atagatttta acgaattaac tttaaagcaa atgatcgtct tattcaaaga
      301 atatccttca ttattgagac gacctatttt gattgatgaa aaaagaatac taatcggatt
      361 taatgaagat gagatccggt catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct
      421 gatctattta caaaacaata acatcttaaa ttcataggga gggagccttc gtggaagtga
      481 aagtctatgg atcagcgcac tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc
      541 atcaattgcc ttatcaattt gttgacttag aagaacgcga tctaagtaca gaagaagcag
      601 aagaattatg cagttttgaa gaagtgcaag cagaacagct atttgcgact tggtctgagg
      661 cttttcacaa cattgcgatt gacctttcct gtgtaggaaa aaaacaactg atttttttat
      721 gcacacaaca taccaatctc ctcagacgtc ctttgattat tatcgatgaa gttctattta
      781 ttggatacaa tgagcagttg atggaacagc ttatcttaaa taaatagact ttaactgttt
      841 taatcaagtc actgagcatt tcatacagtt gtaacagtcg aacacttagc atcaagcgtg
      901 ttatgtgttc tttttttgac tttatttttc tactatagag acatgctcaa tttatgtggt
      961 caaaattcaa atctaggtgg ggatatagag ttaaaaatat attgacatgt gtaaatttac
     1021 atgatatatt catttctgtt gttttaagta gcaaagcaat aaaatagtat tttaaaaaac
     1081 atataaaata gttgttgaca tgttagaaat gacatgataa gataactgag ttgttaaaaa
     1141 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
     1201 acaaacgata agttatgata aatgagttgc tgaaagattt cgaaaaacga aatcgaaaaa
     1261 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
     1321 gcagccaaaa acgaatcg
//
LOCUS       DASOUD010000201         1330 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.209, whole genome shotgun sequence.
ACCESSION   DASOUD010000201 DASOUD010000000
VERSION     DASOUD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.209"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(215..472)
                     /locus_tag="U4A29_002664"
     CDS             complement(215..472)
                     /locus_tag="U4A29_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEO0681746.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(597..929)
                     /locus_tag="U4A29_002665"
     CDS             complement(597..929)
                     /locus_tag="U4A29_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681747.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT          419 a          226 c          176 g          509 t
ORIGIN      
        1 tcttatttaa agttgctata taccctctta ttttagcata ctcagtttta gaaaaagcaa
       61 aaaaataaag aaacttattc ttgataacaa gctacgtgac gtatatccct aacacaaaaa
      121 tagccatatc ctttggtttg gatacggcta tttgatatta ctcattcaat tatcaaatca
      181 cagtaggtgt gaatctcatc gggatcggca atatctactt ctgattagga tcaaaggtga
      241 ttttctcaat aaaggtgtga tacaaagctt taacttgctt cttctcactc tggataagaa
      301 agcaatccaa actagtcaat atctgttgga catctttgac ttgaatcggt tccccttgtt
      361 gttgaaagat ccctaagatt tcattctctc gaatttgtaa ctgtcttcgt ttttcagtca
      421 attcatttaa acgatctttt aacatcggaa acaattcggg actatcttcc aaagcagctt
      481 cctgtttttg ctctaatagt tagattttgc gtaccatttt cttcttttat tttccttcga
      541 ttgctgattt taatatagtc taaaggaaat ttatatttat tgtattcttt ataacttcaa
      601 gagaacttcg tttgagtatt actgtatttt tttaattcca taagaaaatt agttattacc
      661 tgccacctaa tcagcaaatc tgaacctgag tcaatataat taatattatt ttccatctcc
      721 aacattgtaa gtactgttgg tgttaatata gggactttgt acaattgtat ttttttgatt
      781 ttatcatctg cacaaaggag attttttaaa tctaatcctt cttttatttc aaaagttgaa
      841 ttttttaaat ctataagagt aaaaccaatt ccttcatact gaatttctga aaatcgaatt
      901 tttctcccag ttgatagatc aaggggcaga aaacctttta ttacattgtg agtgtcacca
      961 tcatttacaa tgtcattagg aattttgatt ttagaattct gtggagcacc tattggaaga
     1021 taataattca ttataatttt gtagtcttca tgaggaaata aagtactttt aggattgcta
     1081 agatattctc gtaccgaatc taagaatggc ttatccattg aatcttcatc cccatttaaa
     1141 ctaaaaaaca ttcctacaat tgcttttgca actctaagtg aattcatttt ctttattgaa
     1201 tgtgttaaat cagacttacc attctttaaa cttgtattgt tataaaggaa atttttattg
     1261 tcttctgcaa atctaatata ttcctcaaca tactcactac caaaaaaact attacattta
     1321 ctacataacg
//
LOCUS       DASOUD010000202         1276 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.210, whole genome shotgun sequence.
ACCESSION   DASOUD010000202 DASOUD010000000
VERSION     DASOUD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.210"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1203)
                     /locus_tag="U4A29_002666"
     CDS             complement(31..1203)
                     /locus_tag="U4A29_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEO0681748.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          421 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc ttttat
//
LOCUS       DASOUD010000203         1272 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.211, whole genome shotgun sequence.
ACCESSION   DASOUD010000203 DASOUD010000000
VERSION     DASOUD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.211"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..>1272
                     /locus_tag="U4A29_002667"
     CDS             34..>1272
                     /locus_tag="U4A29_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEO0681749.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKS"
BASE COUNT          430 a          251 c          246 g          345 t
ORIGIN      
        1 acaaaataaa caactgagag gacgatacgc tatatgaatg attctatcaa aaaaatgctg
       61 agaataacag agaaagattt gatgattaca gaggtctctt acgagaccct tcagaagaaa
      121 aagacgttgg tcgtcgatgc tgttctctcg cctactcctc gtgcttgtag aagttgtggt
      181 tctactgtgg tagatggaaa cgggaaagca attatagtga aaaatggaaa aaaggaaacg
      241 attgtccgtt ttgaacaata caatcatatg cctttggtta tgcgcctaaa aaagcaacgc
      301 tatacctgta aaaactgtcg cacccattgg acggctcaaa gttattttgt ccaacccaga
      361 cattcaatcg caaatcatgt tagatataaa attgcttctt tactgactga aaaagtatct
      421 ttatctttta ttgcgaaaag ctgtcaggta tctttgacca ccgttattcg tacattgaaa
      481 gagtttaaaa gctatttacc aaagcaatct aagaagatcc tccccagagt attgatggtt
      541 gacgaatttc gttcgcatgc ttccatagaa gataagatga gctttatttg cgcggatggc
      601 gaaacaggaa aattaataga tgttttgcct acgcgtaaat tacctcgatt aacaagctat
      661 ttcttaggtt gtaccaatcc agaagaagta gaattcttgg tgacagacat gaacgccgcc
      721 tacttccagc tcaccaaacg tgttctgcca aatgcgaaag tcgtgattga tcggtttcat
      781 attgtcaaac acatgaatca agcgttcaat gagttgcgta tccgtgaaat gaatgaactt
      841 cgtaaagccg gacagaaaag ccaggcagaa aaactgaaaa agaactggcg ctttttgcta
      901 aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt ccgagcacca
      961 aaatacccat ttcttactga agcaatgatg attgatcgat tgcttgagtt ttctccgccc
     1021 ctaaaggagg cgtatccctt ttttcatgaa ttagttgaag cctttcgaga caaagaccct
     1081 gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacag ctttcgggaa
     1141 aagcttcaaa accttctgac ctatgaagaa ggcatcacca acgcaatgat ctatccttat
     1201 tccaatggaa aaatagaagc gaagaatacc cacataaaga caatgaaacg agtatcctac
     1261 ggatttaaat ca
//
LOCUS       DASOUD010000204         1247 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.212, whole genome shotgun sequence.
ACCESSION   DASOUD010000204 DASOUD010000000
VERSION     DASOUD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.212"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="U4A29_002668"
     CDS             200..742
                     /locus_tag="U4A29_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681750.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1247
                     /locus_tag="U4A29_002669"
     CDS             898..>1247
                     /locus_tag="U4A29_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEO0681751.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          515 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccaga
//
LOCUS       DASOUD010000205         1118 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.213, whole genome shotgun sequence.
ACCESSION   DASOUD010000205 DASOUD010000000
VERSION     DASOUD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.213"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..243
                     /locus_tag="U4A29_002670"
     CDS             <1..243
                     /locus_tag="U4A29_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO0681752.1"
                     /translation="CASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKII
                     RIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            296..1033
                     /gene="aph(2'')-I"
                     /locus_tag="U4A29_002671"
                     /pseudo
     CDS             296..1033
                     /gene="aph(2'')-I"
                     /locus_tag="U4A29_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
BASE COUNT          470 a          110 c          187 g          351 t
ORIGIN      
        1 tgcgcatcat tggatgatgg attcgaagac gcctttcaat ataccgtaca aggaaattcc
       61 cacaatcgac taaagagtac caatctaatt gaacgactga atcaagaagt acgcagaaga
      121 gaaaagatta ttcgcatctt ccccaatcaa acatcagcca atcgcttaat tggagccgtt
      181 cttatggacc tacatgatga atggatttat tcttcaagaa aatacatcaa ttttgataag
      241 tagaaatggt aaaaacattg tatagcattt tacacaggag tctggacttg actaaaaagg
      301 ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa ctaatgtaaa
      361 aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag gttataaaga
      421 aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag aagaacaaaa
      481 tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag attatacaga
      541 tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata tattgttgcg
      601 tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa gttttatgga
      661 aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata atgattttag
      721 ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg attttggaga
      781 ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata gtgaagaaga
      841 aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag atattgagaa
      901 agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta ttgtttatgg
      961 aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt ataaaaggac
     1021 ttataaagat tgattatata atatatgaaa agctattata aaagacatta gtattaaata
     1081 gtttaaaaaa atgaaaaata ataaaggaag tgagtcaa
//
LOCUS       DASOUD010000206         1090 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.214, whole genome shotgun sequence.
ACCESSION   DASOUD010000206 DASOUD010000000
VERSION     DASOUD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.214"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="U4A29_002672"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="U4A29_002672"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEO0681753.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          383 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta
//
LOCUS       DASOUD010000207         1063 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.215, whole genome shotgun sequence.
ACCESSION   DASOUD010000207 DASOUD010000000
VERSION     DASOUD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.215"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..993)
                     /locus_tag="U4A29_002673"
                     /pseudo
     CDS             complement(37..993)
                     /locus_tag="U4A29_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          347 a          178 c          159 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcattcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aac
//
LOCUS       DASOUD010000208         1062 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.216, whole genome shotgun sequence.
ACCESSION   DASOUD010000208 DASOUD010000000
VERSION     DASOUD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.216"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            261..794
                     /locus_tag="U4A29_002674"
     CDS             261..794
                     /locus_tag="U4A29_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEO0681754.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          343 a          197 c          194 g          328 t
ORIGIN      
        1 gtttagtgga gaagttgtga aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa
       61 aatggttcct cacaactttg cataaatttg acttaatacc gagaagtcat gatccccatc
      121 taactccaaa tcacaaggaa tgagttcaaa aactagcttt tctcaacaaa tattacataa
      181 aaaattctat gttttttgtt aaatttattt gcttttttca aaagtatgtt acagtaattt
      241 cccaaggagg ttttacagaa atgaattttg ttgccatgga ttttgaaact gccaatcatc
      301 agccttatag tgcttgttct cttgcgctag tcatggtaaa aaatagccag atcgttgatg
      361 aattctatac attgatacag cctgagactc cttttttctg gcgaaatgtc caaattcacg
      421 gaatccatca agaagacgta cgcaatgcgc cgaaattccc agaagtctgg caaactatcc
      481 aaccatattt tcaaaaaaat cgtttagtcg ttgcacataa tgctgctttt gatacaaagg
      541 tcttagcggg atgcttagat tattatcatc ttgaacagcc taattatctt tctttatgta
      601 cagtgaaaag tagccgtcgc ctgtttcctg aatttccaaa tcatcgattg aacactgtgt
      661 gcgaaaattt gaacatccaa ttagatcatc accatgatgc attggaagat agtcgtgcat
      721 gtgcggaaat cctgctttat caagaaaaaa aattcgggac agatccattg aaaaaacttg
      781 tgactataaa gtaaaagaat acttatgaaa cacacaaaga ggtcgtgagc ctgccgtttc
      841 actccgagtc acaaggagca atttcaaaaa atagcttctc atgtttatta aatattgtga
      901 ctggtgaccg aggagctggc aggagaacac cgtttattgg agaggttgtg aaaaagctgt
      961 tctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcatttggtt
     1021 ggacttatta ccgcagatgc aagcttttgg acaccgttta cc
//
LOCUS       DASOUD010000209         1035 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.217, whole genome shotgun sequence.
ACCESSION   DASOUD010000209 DASOUD010000000
VERSION     DASOUD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.217"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="U4A29_002675"
     rRNA            complement(<1..>1035)
                     /locus_tag="U4A29_002675"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DASOUD010000210          984 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.218, whole genome shotgun sequence.
ACCESSION   DASOUD010000210 DASOUD010000000
VERSION     DASOUD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.218"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..602)
                     /locus_tag="U4A29_002676"
     CDS             complement(<1..602)
                     /locus_tag="U4A29_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEO0681755.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          323 a          174 c          188 g          299 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaattt gatcaaagtg gtagctgtta ttgaattcaa gaattgtttc tcgtatgaat
      481 gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa ttgattgtcg
      541 tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac aaataatttc
      601 atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac tgtgggattt
      661 cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat aaaagagcta
      721 gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca gctaatgagt
      781 tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac gctacaggag
      841 gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga gaaatcacac
      901 aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa tctagaaaca
      961 ttttgaataa aaactattag acaa
//
LOCUS       DASOUD010000211          974 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.219, whole genome shotgun sequence.
ACCESSION   DASOUD010000211 DASOUD010000000
VERSION     DASOUD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.219"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..974
                     /locus_tag="U4A29_002677"
                     /pseudo
     CDS             1..974
                     /locus_tag="U4A29_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          359 a          166 c          187 g          262 t
ORIGIN      
        1 gaactaacgg cttcagcatt tgacaaatgg gtaaagcaat ctaaaacgag tggttcattc
       61 aaagaaaaag ataatcttac gcctgaacaa aaagaattgt tagaactacg taaaagaaac
      121 cagcaattag aaatggaaaa tgatatttta aagcaagcag cgctgatatt cggacgaaga
      181 gacaagtaat cgatgcgaat aagcatcttt accctatatc agcgatgtgc agaatattag
      241 gtctatcacg tcagtcctat tattatcaat caaaaccaaa gaaagacgaa tcagaacttg
      301 aagaagtagt cgctgaagaa tttatccgca gccgaaaggc ctacggctca agaaaaataa
      361 aaaaagcctt atcaaaacga ggcattcaga tcagccgacg aaaaattagt agaatcatga
      421 aaaatagagg attaaaatcg agctatactg ttgcttattt taaagtacat cattctactt
      481 gcaatgaagc caaaacgaca aacgtattga atcgtaaatt cttaagagac aacccattag
      541 aagcgatcgt aacagacttg acttatgtac gagtcgggaa aaaatggaat tatgtctgtt
      601 tcattttgga tctgttcaat cgagaaattc tcggctattc ttgtggagaa cataaagatg
      661 ccgttctagt aaaaaaagca tttagccgta tcaaacaacc tctgacagag gttgagattt
      721 ttcatactga tcgtggaaaa gagtttgata accaagctat tgatgaatta ttaacaactt
      781 ttgacatcaa tcgatcattg agtcataaag gctgtccttt tgataatgcc gtagctgaat
      841 caacttataa gtcgttgaaa gtagaatttg tctatcaata cacatttgaa accttacaac
      901 aattggattt ggagttattt gactatgtca attggtggaa ccaccttcgg ttgcacggta
      961 cacttggcta cgag
//
LOCUS       DASOUD010000212          943 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.220, whole genome shotgun sequence.
ACCESSION   DASOUD010000212 DASOUD010000000
VERSION     DASOUD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.220"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>943
                     /locus_tag="U4A29_002678"
     rRNA            <1..>943
                     /locus_tag="U4A29_002678"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DASOUD010000213          876 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.222, whole genome shotgun sequence.
ACCESSION   DASOUD010000213 DASOUD010000000
VERSION     DASOUD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.222"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..434)
                     /locus_tag="U4A29_002679"
     CDS             complement(<1..434)
                     /locus_tag="U4A29_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO0681756.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="U4A29_002680"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="U4A29_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEO0681757.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          263 a          143 c          137 g          333 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttg
//
LOCUS       DASOUD010000214          785 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.223, whole genome shotgun sequence.
ACCESSION   DASOUD010000214 DASOUD010000000
VERSION     DASOUD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.223"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(55..127)
                     /locus_tag="U4A29_002681"
     tRNA            complement(55..127)
                     /locus_tag="U4A29_002681"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:His,seq:gtg)
     gene            complement(130..203)
                     /locus_tag="U4A29_002682"
     tRNA            complement(130..203)
                     /locus_tag="U4A29_002682"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(169..171),aa:Trp,seq:cca)
     gene            complement(210..290)
                     /locus_tag="U4A29_002683"
     tRNA            complement(210..290)
                     /locus_tag="U4A29_002683"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(254..256),aa:Tyr,seq:gta)
     gene            complement(292..367)
                     /locus_tag="U4A29_002684"
     tRNA            complement(292..367)
                     /locus_tag="U4A29_002684"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(332..334),aa:Phe,seq:gaa)
     gene            complement(403..475)
                     /locus_tag="U4A29_002685"
     tRNA            complement(403..475)
                     /locus_tag="U4A29_002685"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Asp,seq:gtc)
     gene            complement(485..557)
                     /locus_tag="U4A29_002686"
     tRNA            complement(485..557)
                     /locus_tag="U4A29_002686"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Val,seq:tac)
     gene            complement(573..644)
                     /locus_tag="U4A29_002687"
     tRNA            complement(573..644)
                     /locus_tag="U4A29_002687"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Glu,seq:ttc)
     gene            complement(659..748)
                     /locus_tag="U4A29_002688"
     tRNA            complement(659..748)
                     /locus_tag="U4A29_002688"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Ser,seq:gga)
BASE COUNT          215 a          215 c          183 g          172 t
ORIGIN      
        1 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa aaagggcgaa
       61 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
      121 cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg gagaccgctg
      181 ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac cactgaaccc
      241 taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc atggtggcgc
      301 gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc aactgagcta
      361 ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg acgggaatcg
      421 aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaaccagttc
      481 attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
      541 agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg aacccgtgtt
      601 accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg
      661 gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat ttccaatcat
      721 gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact ctttctcaaa
      781 atgag
//
LOCUS       DASOUD010000215          775 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.224, whole genome shotgun sequence.
ACCESSION   DASOUD010000215 DASOUD010000000
VERSION     DASOUD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.224"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..660
                     /locus_tag="U4A29_002689"
     CDS             <1..660
                     /locus_tag="U4A29_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO0681758.1"
                     /translation="YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFT
                     SKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNK
                     LSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKV
                     DKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          285 a          120 c          143 g          227 t
ORIGIN      
        1 tatatttctt tgttgtgtaa agaagaaatt atcgaattac ctaaaactaa ttccacaatt
       61 ggtattgact taggtattat ggactttgcg attctttctg atggacaaaa aattgataat
      121 aataaattca cgtccaaaat ggaaaagaaa ctaaagcgtg aacaacgcaa attgtctagg
      181 cgtgctttat tggctaaaaa taaaggtacc aatctatttg aagctaaaaa ttatcaaaaa
      241 caaaaacgca aagtagctag attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat gatcaaaaat cacgatatta tctgtattga agacttaaac
      361 acaaaaggta tgttgcgaaa tcataaatta gctaaaagta tttctgatgt ttcttggtca
      421 aagtttgtga ctaaattaca atacaaggct gactggtatg gacgaaaaat catcaaagta
      481 gataaatggt ttccgtctag tcagatttgt tcagagtgcg gacataaaga tggcaagaaa
      541 tctcttgaaa ttcgagaatg gacttgtcct gtttgtcata ctcatcatga tcgtgatatc
      601 aatgctagta tcaatattct gaccgaaggt ctaagactac attctatggg attggcttag
      661 atccaatcat caagaaccgt aggaactacg gggatagctt ggtaaataag agacaccgct
      721 gttagtaaag aaatacgcta tcaagtatgc tctattccca agaagctccc acttc
//
LOCUS       DASOUD010000216          768 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.225, whole genome shotgun sequence.
ACCESSION   DASOUD010000216 DASOUD010000000
VERSION     DASOUD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.225"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..146)
                     /locus_tag="U4A29_002690"
     CDS             complement(<1..146)
                     /locus_tag="U4A29_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681759.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGR"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="U4A29_002691"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="U4A29_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEO0681760.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..>768)
                     /locus_tag="U4A29_002692"
     CDS             complement(591..>768)
                     /locus_tag="U4A29_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681761.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          222 a          156 c          135 g          255 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatc
//
LOCUS       DASOUD010000217          725 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.227, whole genome shotgun sequence.
ACCESSION   DASOUD010000217 DASOUD010000000
VERSION     DASOUD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.227"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..606
                     /locus_tag="U4A29_002693"
     CDS             <1..606
                     /locus_tag="U4A29_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEO0681762.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          269 a          139 c          127 g          190 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag
      601 aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga
      661 tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca
      721 tcttg
//
LOCUS       DASOUD010000218          725 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.228, whole genome shotgun sequence.
ACCESSION   DASOUD010000218 DASOUD010000000
VERSION     DASOUD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.228"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..716
                     /locus_tag="U4A29_002694"
     CDS             <1..716
                     /locus_tag="U4A29_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO0681763.1"
                     /translation="FTGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLS
                     KEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDY
                     ELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQA
                     YNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYT
                     HTTKNMQNQLVEKLGKIEI"
BASE COUNT          309 a          102 c           97 g          217 t
ORIGIN      
        1 cttttacagg aatgattttt aaattcgcca accgtcgata tggattaaaa tataatcccg
       61 taaatggggt ttctttagca agaaaaccta agacaaacga agaaataaaa aaagaaaaag
      121 atggttattt atcaaaagaa gaagttcaaa tgattgctag taaacaaaaa agttcacatc
      181 aacagtcacg ttattcttta attactaaat ttttgttttt aactggacta cgttatggtg
      241 aattgatatc tttgactgaa tctgattatg atggtaataa aattacggtt tctggtactt
      301 atgattacga gttaaaaatt aaaacaacaa ctaaaaatac aggttcttat agaaccattg
      361 aattatcaag taatgctaaa gaaatcatag atcaattaat tgaagaaaac aaattaatta
      421 agaataataa agataaatac attttcatct ctaaaaatgg taacccgata tctattcaag
      481 catataatca atctttaaga gcagtatcca aagaattaaa catagataaa aaagtttctt
      541 ctcacatgtt aaggcattcc cacatttctc tacttacaga attaggaatc ccattaaaag
      601 ccattatgga tcgtgtagga catgaagatt caaaaacaac attaaagatt tatactcata
      661 caactaaaaa tatgcaaaat caactagtag aaaaactagg aaaaatagaa atttaaattg
      721 cccct
//
LOCUS       DASOUD010000219          697 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.229, whole genome shotgun sequence.
ACCESSION   DASOUD010000219 DASOUD010000000
VERSION     DASOUD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.229"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..629)
                     /locus_tag="U4A29_002695"
     CDS             complement(54..629)
                     /locus_tag="U4A29_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO0681764.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          125 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DASOUD010000220          663 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.230, whole genome shotgun sequence.
ACCESSION   DASOUD010000220 DASOUD010000000
VERSION     DASOUD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.230"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            45..>663
                     /locus_tag="U4A29_002696"
     CDS             45..>663
                     /locus_tag="U4A29_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEO0681765.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISN"
BASE COUNT          230 a          116 c          126 g          191 t
ORIGIN      
        1 cttatttcaa aatgaatcaa tctgttgcta aaactgccca ttgcttgaat cgttcaagac
       61 aaacgatcca taaagtatat ctgttcttca agcaaggaaa atcagcctta gaatattatc
      121 aacagtataa gaaaaacaaa tcaaactgtg gtagacgtcc gcttgtttta cccgaggaac
      181 aatcagaata tattcaaaga aaggttgttc aaggatggac acccgatgtg attgttggtc
      241 gtgcagcgtt tcctattagt tgttctgctc gtaccattta tcgtatgttc aaaaaggggc
      301 tatttgattc ttctgactta ccgatgaaag gcaagcgtaa accgaatgga catcaagaaa
      361 gacgtggaaa acaaactttc cgccgctcta ttcatgaacg tgaaaaggat tatagccaat
      421 tctcaaatga gtttggtcac cttgaaggtg acactatcgt aggtctgaaa cataaaagtg
      481 ctgtaattac cttagttgaa cgattatcaa aagttatcat cacattgaaa ccgtgtggta
      541 gacaagcgat tgatattgaa aaaaaattaa atcaatggtt tgaatctgta ccgaaaaacc
      601 tattcaaatc catcactttt gattgtggaa aggaattttc aaattggaaa cagatcagta
      661 atg
//
LOCUS       DASOUD010000221          612 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.231, whole genome shotgun sequence.
ACCESSION   DASOUD010000221 DASOUD010000000
VERSION     DASOUD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.231"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..430)
                     /locus_tag="U4A29_002697"
     CDS             complement(<1..430)
                     /locus_tag="U4A29_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO0681766.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          189 a          113 c           82 g          228 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcaacaagag ggctatttaa ataccacatt gtttcctctt taaaataagt gtctcgagtt
      541 tcattgggaa cagagattta caagaaaaac taacttaata aaattcgata atcatgtgaa
      601 tttataatat aa
//
LOCUS       DASOUD010000222          595 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.232, whole genome shotgun sequence.
ACCESSION   DASOUD010000222 DASOUD010000000
VERSION     DASOUD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.232"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            91..>595
                     /locus_tag="U4A29_002698"
     CDS             91..>595
                     /locus_tag="U4A29_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681767.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQ"
BASE COUNT          213 a          105 c          127 g          150 t
ORIGIN      
        1 aaaatacata ttttaaatat gaaagtacaa ttggaaatag attaaaacga taaaataaaa
       61 ttgtcaaaac gtcagagagg atggtggaga atgaatcaat gtgtaaaaaa gctgattgcg
      121 tcgattttgt ttgtcttccc tctcttgatg gtgacatccg tttttgccga tgtagagaaa
      181 acaaacgaat caacgcaatc agagaatcaa agttttgaca agactacttt ttcagaagaa
      241 tcgattactt ctgaaactac agaatctcaa agtgaggctt ctactaataa aaggcaagaa
      301 gaaaataaga caagtgagac ctcagaagta gctgaacaaa cgtctgatag cagtgaagcg
      361 gcactggcaa gagctgtttc agcggatggg tacagcgaag ctgccacaac agaagaactg
      421 gcgcagcttc tcgcagacga atcagtaact aagatccgac tgattcagcc tttgacttta
      481 gacagggaat tagagatcaa acgagatatc gttattgatt ttggtggatt cgctcacaat
      541 ttcggcacac accatattta tatcaatgag atacctcagt cgatcgagtt ccaga
//
LOCUS       DASOUD010000223          574 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.233, whole genome shotgun sequence.
ACCESSION   DASOUD010000223 DASOUD010000000
VERSION     DASOUD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.233"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..572
                     /locus_tag="U4A29_002699"
     CDS             <1..572
                     /locus_tag="U4A29_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEO0681768.1"
                     /translation="IRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIK
                     GRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQY
                     QMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLN
                     EYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          248 a           71 c           94 g          161 t
ORIGIN      
        1 aaatccgaat aaagcgatat aaatcttata aaggaacagt aggaaaaatt gccaagaatg
       61 tgttaaaacg aaatttttca gtagatacac ccaataaaaa atgggtgaca gacgtcactg
      121 aattcaagat aaaaggaaga aaaatctatc tatctcctat acttgattta tttaacggag
      181 aaataattag ttatagtata tcaactagtc caacatataa actgattgaa gaaatgcttc
      241 aacaagcaat taaaaaaaag ggaactgagg gttcactgat tctacattca gatcaaggat
      301 ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga gaataacatt attcaaagca
      361 tgtctagaaa aggaaattgc ttagataatt ctgtaataga aaattttttt ggtgtgctta
      421 aatcagagtt tttttatcga gaaaaattcc gatcaattga gatatttcaa agtaaattaa
      481 atgagtatat taggtggtat aataacaaaa ggataaaact aaagttaaat ggactatctc
      541 cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DASOUD010000224          570 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.234, whole genome shotgun sequence.
ACCESSION   DASOUD010000224 DASOUD010000000
VERSION     DASOUD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.234"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(9..440)
                     /locus_tag="U4A29_002700"
     CDS             complement(9..440)
                     /locus_tag="U4A29_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEO0681769.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          201 a           73 c           65 g          231 t
ORIGIN      
        1 ataaattttt agttattaac ttgattaaaa tcagagataa tatcatttaa ttcagtttga
       61 agggttgtaa cgattaaatg gaaattttct tcatctgttt tattatatgg ggatgttttc
      121 gttatatata acaacttttc tttttcaatt ttcattaata actctgtcga agtctgtact
      181 ttatcttgat aattaaatgc agacatacca ctttgtataa ttgtaattat tgctgaagca
      241 attgaaacaa tcaatagtaa agacctgtgt tcggaagcct gatcaattaa tattgggata
      301 ctagctgata agactatttt tataatattt ccgataccaa ttactctatt ataaaatcga
      361 atttcttttt ttaatctatc gattgtagaa tcaatagatt cgataaacgc tttttgatcc
      421 atcattttct ctccaatcat tttattaaaa tcattaacaa aaatattata ataggattta
      481 agtaagttct aactaatcac atataaaaat aaaaaaacat ttgtatttta tcaaagtttt
      541 attatacttg tcaatttgtg tacatcctaa
//
LOCUS       DASOUD010000225          556 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.235, whole genome shotgun sequence.
ACCESSION   DASOUD010000225 DASOUD010000000
VERSION     DASOUD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.235"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..450)
                     /locus_tag="U4A29_002701"
     CDS             complement(<1..450)
                     /locus_tag="U4A29_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO0681770.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          155 a          102 c           70 g          229 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctt
//
LOCUS       DASOUD010000226          542 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.236, whole genome shotgun sequence.
ACCESSION   DASOUD010000226 DASOUD010000000
VERSION     DASOUD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.236"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            141..398
                     /locus_tag="U4A29_002702"
     CDS             141..398
                     /locus_tag="U4A29_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681771.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          231 a           67 c           76 g          168 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tt
//
LOCUS       DASOUD010000227          537 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.237, whole genome shotgun sequence.
ACCESSION   DASOUD010000227 DASOUD010000000
VERSION     DASOUD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.237"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>537
                     /locus_tag="U4A29_002703"
     CDS             <1..>537
                     /locus_tag="U4A29_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEO0681772.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDH
                     RPVKRRNKFYRSLRTASTT"
BASE COUNT          185 a           96 c          104 g          152 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt
      421 ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca
      481 tcgtccagta aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccac
//
LOCUS       DASOUD010000228          521 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.238, whole genome shotgun sequence.
ACCESSION   DASOUD010000228 DASOUD010000000
VERSION     DASOUD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.238"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..405)
                     /locus_tag="U4A29_002704"
     CDS             complement(<1..405)
                     /locus_tag="U4A29_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEO0681773.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASD"
BASE COUNT          162 a           94 c           69 g          196 t
ORIGIN      
        1 atcactagca tcaacatctt taccccaatc atagtgaaca ggatattcag ctgcttccca
       61 tagaggagat ccttcttcat tagataaaca aatagtcatt gctccaaatt ctcttgcttt
      121 ttttgttgct tctacagttt ctggtgttgt gccagaatgg gaacaagtga taactaaaga
      181 attttcagat aatccttttg gtatagcata gttaaattcg ttagcagtat aaacatgaca
      241 aggtacagtt aattctttat caaatagata ttgaccagcc attaaaacgg cttgtgaacc
      301 cccacatgct gtaaaatata catgatcaat tgtcttttcc tttaacgctg ccattgctgt
      361 ttcaatctgt tctttgtgta gttgtctcat ttctttatcc ctcactctaa taggtattat
      421 aaagttatat aactttacga tacctattat ctatgaaatt gctttatttt gcaatactat
      481 ttttcaaaaa aattttttat aacaatttta tataataaaa t
//
LOCUS       DASOUD010000229          511 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.239, whole genome shotgun sequence.
ACCESSION   DASOUD010000229 DASOUD010000000
VERSION     DASOUD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.239"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(118..189)
                     /locus_tag="U4A29_002705"
     tRNA            complement(118..189)
                     /locus_tag="U4A29_002705"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Glu,seq:ttc)
     gene            complement(201..274)
                     /locus_tag="U4A29_002706"
     tRNA            complement(201..274)
                     /locus_tag="U4A29_002706"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(238..240),aa:Ile,seq:gat)
     gene            complement(287..357)
                     /locus_tag="U4A29_002707"
     tRNA            complement(287..357)
                     /locus_tag="U4A29_002707"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Gly,seq:tcc)
     gene            complement(362..434)
                     /locus_tag="U4A29_002708"
     tRNA            complement(362..434)
                     /locus_tag="U4A29_002708"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:Phe,seq:gaa)
     gene            complement(438..510)
                     /locus_tag="U4A29_002709"
     tRNA            complement(438..510)
                     /locus_tag="U4A29_002709"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(475..477),aa:Asp,seq:gtc)
BASE COUNT          163 a          137 c          107 g          104 t
ORIGIN      
        1 ttacattaga actgtccaaa aaagcttagc acaatcatcc ataaagataa ctctgctaag
       61 ctttttaaaa tcattctttc agataaaaag aaaaagatac tcaaaaagta tcttccatga
      121 cccgtacggg attcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac
      181 caacgggcca ataaaataaa tgggcctaaa tggactcgaa ccatcgacct cacgcttatc
      241 aggcgtgcgc tctaaccagc tgagctatag gcccataaaa actaaaagcg ggtgacgaga
      301 atcgaactcg cgacaacagc ttggaaggct gtggttttac cactaaacta cacccgcata
      361 atggcgcggg acagaatcga actgccgaca catggagctt caatccattg ctctaccaac
      421 tgagctaccg agccaaacgg ttccgacggg aatcgaaccc gcgatctcct gcgtgacagg
      481 caggcatgtt aacccctaca ccacggaacc a
//
LOCUS       DASOUD010000230          510 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.240, whole genome shotgun sequence.
ACCESSION   DASOUD010000230 DASOUD010000000
VERSION     DASOUD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.240"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          229 a           70 c           82 g          129 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaaact tcaaaaaagt tcttgacaaa
      361 taacaaacga taagttatga taaatgagtt gccgaaagat ttcgaaaaac gaaatcgaaa
      421 aaaactttta aaaaagagtt gacaatcgct tgtctctctg atatgatata aaagttgcta
      481 cggcagccaa aaatgaatcg ggaaacactc
//
LOCUS       DASOUD010000231          494 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.241, whole genome shotgun sequence.
ACCESSION   DASOUD010000231 DASOUD010000000
VERSION     DASOUD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.241"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..325
                     /locus_tag="U4A29_002710"
     CDS             <1..325
                     /locus_tag="U4A29_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0681774.1"
                     /translation="KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQR
                     EGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
BASE COUNT          197 a           66 c           90 g          141 t
ORIGIN      
        1 aaaggcaagt ttaaaatcac taaaagatac atggcttgat ttatcagaag ataatccata
       61 cagccaattc ttaattactg taatggctgg tgttaaccaa ttagagcgag atcttattcg
      121 gatgagacaa cgtgaaggga ttgaattggc taagaaagaa ggaaagttta aaggtcgatt
      181 aaagaagtat cataaaaatc acgcaggaat gaattatgcg gtaaagctat ataaagaagg
      241 aaatatgact gtaaatcaaa tttgtgaaat tactaatgta tctagggctt cattatacag
      301 gaaattatca gaagtgaata attagccatt ctgtattccg ctaatgggca atatttttaa
      361 agaagaaaag gaaactataa aatattaaca gcctcctagc gatgccgaaa agccctttga
      421 taaaaaaaga atcatcatct taagaaattc ttagtcattt attatgtaaa tgcttataaa
      481 ttcggcccta taat
//
LOCUS       DASOUD010000232          477 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.242, whole genome shotgun sequence.
ACCESSION   DASOUD010000232 DASOUD010000000
VERSION     DASOUD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.242"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>477
                     /locus_tag="U4A29_002711"
     CDS             <1..>477
                     /locus_tag="U4A29_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEO0681775.1"
                     /translation="IIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSS
                     NLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKK
                     SYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          157 a           86 c           85 g          149 t
ORIGIN      
        1 tgattattgc ttgtgttata tggggcataa ttaatggcta tactagccaa agagccacgt
       61 atagagcggt ttgttccgtc ttatttccta atggtgactt tcctagtagg agtcgattca
      121 ctcgcttaag ttcaaactta gcttacacta tcaagattat tcgatacttt ttcatcaaga
      181 agctcaccaa aggtgaacta gtcggaatta tagatagttt tcctagtcca ttatgtaaac
      241 cagttagaaa tagacaagca aaactattga atcaaatagc caaagttggc tataactcaa
      301 caaaaaaatc ttatttttat ggtcttaaaa ttcatatgat cgttactaag actggctttc
      361 cgattactta ctcaatcacg aatccaggtg tccatgacgt gaaagtgtta gagactttat
      421 cggaagaagc gaatcttccc aatatcctag gggacaaagg atatattagc cataaaa
//
LOCUS       DASOUD010000233          460 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.243, whole genome shotgun sequence.
ACCESSION   DASOUD010000233 DASOUD010000000
VERSION     DASOUD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.243"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..91)
                     /locus_tag="U4A29_002712"
     tRNA            complement(20..91)
                     /locus_tag="U4A29_002712"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="U4A29_002713"
     tRNA            complement(113..200)
                     /locus_tag="U4A29_002713"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="U4A29_002714"
     tRNA            complement(225..298)
                     /locus_tag="U4A29_002714"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="U4A29_002715"
     tRNA            complement(312..382)
                     /locus_tag="U4A29_002715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="U4A29_002716"
     tRNA            complement(384..459)
                     /locus_tag="U4A29_002716"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DASOUD010000234          438 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.244, whole genome shotgun sequence.
ACCESSION   DASOUD010000234 DASOUD010000000
VERSION     DASOUD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.244"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            85..381
                     /locus_tag="U4A29_002717"
     CDS             85..381
                     /locus_tag="U4A29_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEO0681776.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          171 a           64 c           75 g          128 t
ORIGIN      
        1 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
       61 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
      121 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
      181 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
      241 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
      301 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
      361 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
      421 ggtcagctga ttgaacat
//
LOCUS       DASOUD010000235          399 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.245, whole genome shotgun sequence.
ACCESSION   DASOUD010000235 DASOUD010000000
VERSION     DASOUD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.245"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DASOUD010000236          394 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.246, whole genome shotgun sequence.
ACCESSION   DASOUD010000236 DASOUD010000000
VERSION     DASOUD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.246"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..285
                     /locus_tag="U4A29_002718"
     CDS             <1..285
                     /locus_tag="U4A29_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO0681777.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DASOUD010000237          381 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.247, whole genome shotgun sequence.
ACCESSION   DASOUD010000237 DASOUD010000000
VERSION     DASOUD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.247"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..94)
                     /locus_tag="U4A29_002719"
     tRNA            complement(21..94)
                     /locus_tag="U4A29_002719"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="U4A29_002720"
     tRNA            complement(108..181)
                     /locus_tag="U4A29_002720"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="U4A29_002721"
     tRNA            complement(209..281)
                     /locus_tag="U4A29_002721"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="U4A29_002722"
     tRNA            complement(301..374)
                     /locus_tag="U4A29_002722"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DASOUD010000238          352 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.248, whole genome shotgun sequence.
ACCESSION   DASOUD010000238 DASOUD010000000
VERSION     DASOUD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.248"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>352
                     /locus_tag="U4A29_002723"
     CDS             <1..>352
                     /locus_tag="U4A29_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO0681778.1"
                     /translation="AINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGE
                     LTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYT
                     PQTMSNMTKVLTEEV"
BASE COUNT          131 a           65 c           59 g           97 t
ORIGIN      
        1 agcgataaac acactcctac ggactgaatt aacggctttt ttagattacg aaaaatatga
       61 tcgcactggt tttaattcag gtaattcgag aaacggttct tactttcgat caatcaaaac
      121 cgaatatggt gaattaacat tggaaatacc tagagatcgt aatggtgagt ttaaacaaca
      181 aactttacca gcctacaaaa gaacaaacga tacattggaa accactatta tccatttatt
      241 cgaaaaaggt gttacgatgt ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca
      301 ctatactcca caaaccatgt ccaacatgac taaagttctg actgaagaag ta
//
LOCUS       DASOUD010000239          349 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.249, whole genome shotgun sequence.
ACCESSION   DASOUD010000239 DASOUD010000000
VERSION     DASOUD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.249"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(11..83)
                     /locus_tag="U4A29_002724"
     tRNA            complement(11..83)
                     /locus_tag="U4A29_002724"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Thr,seq:tgt)
     gene            complement(101..182)
                     /locus_tag="U4A29_002725"
     tRNA            complement(101..182)
                     /locus_tag="U4A29_002725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Leu,seq:tag)
     gene            complement(190..262)
                     /locus_tag="U4A29_002726"
     tRNA            complement(190..262)
                     /locus_tag="U4A29_002726"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Lys,seq:ttt)
     gene            complement(266..338)
                     /locus_tag="U4A29_002727"
     tRNA            complement(266..338)
                     /locus_tag="U4A29_002727"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(303..305),aa:Val,seq:tac)
BASE COUNT           98 a           96 c           77 g           78 t
ORIGIN      
        1 tgtttaaaga tgccggctaa aggacttgaa ccctcgaccc tctgattaca aatcagatgc
       61 tctaccaact gagctaagcc ggcatgtaaa acttaaatta tgcgggtgaa gggacttgaa
      121 cccccacgcc gtaaggcgct agatcctaaa tctagtgcgt ctgccaattc cgccacaccc
      181 gcaaaaatat gagccgtaca gggctcgaac ctgtgaccct ctgattaaaa gtcagatgct
      241 ctaccaactg agctaacggc tcatatggag gttaacggga tcgaaccgct gaccctctgc
      301 ttgtaaggca gatgctctcc cagctgagct aaacctccat ggataactt
//
LOCUS       DASOUD010000240          343 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.250, whole genome shotgun sequence.
ACCESSION   DASOUD010000240 DASOUD010000000
VERSION     DASOUD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.250"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          112 a           65 c           56 g          110 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tgcgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaagagg tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca
      241 actcctcggt cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct
      301 ctttgtgact cagagtgaaa cggcaggctc acgacctctt tct
//
LOCUS       DASOUD010000241          341 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.251, whole genome shotgun sequence.
ACCESSION   DASOUD010000241 DASOUD010000000
VERSION     DASOUD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.251"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..269
                     /locus_tag="U4A29_002728"
                     /pseudo
     CDS             1..269
                     /locus_tag="U4A29_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          123 a           46 c           64 g          108 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc c
//
LOCUS       DASOUD010000242          299 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.252, whole genome shotgun sequence.
ACCESSION   DASOUD010000242 DASOUD010000000
VERSION     DASOUD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.252"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>299
                     /locus_tag="U4A29_002729"
     CDS             <1..>299
                     /locus_tag="U4A29_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEO0681779.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DASOUD010000243          299 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.253, whole genome shotgun sequence.
ACCESSION   DASOUD010000243 DASOUD010000000
VERSION     DASOUD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.253"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            166..239
                     /locus_tag="U4A29_002730"
     tRNA            166..239
                     /locus_tag="U4A29_002730"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Ile,seq:cat)
BASE COUNT          118 a           42 c           52 g           87 t
ORIGIN      
        1 cattttttct aaaataagca aataaaaatt aaagtaacaa aatagttatg acacaagtaa
       61 aagaaataag aataacaata ttattttact tatattttga aacttgtgag agtttgtcat
      121 tcgtgctata gttgaaaagt aacaaaaata aattttattt atgatgtacc tttagctcag
      181 ttggttagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta caaggtacat
      241 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagc
//
LOCUS       DASOUD010000244          288 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.254, whole genome shotgun sequence.
ACCESSION   DASOUD010000244 DASOUD010000000
VERSION     DASOUD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.254"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..>288
                     /locus_tag="U4A29_002731"
     CDS             34..>288
                     /locus_tag="U4A29_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEO0681780.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRL"
BASE COUNT           99 a           46 c           64 g           79 t
ORIGIN      
        1 acaaaataaa caactgagag gacgatatgc tatatgaatg attctatcaa aaaaatgctg
       61 cgattaatag agaaagattt gatgattaca gaggtctctt acgagacctt tcagaagaaa
      121 aagacgttga tcgtcgatgc tgttttctca cctgctcccc atacctgtag aaattgtggt
      181 tctactgtgg tagatggaaa cgggaaagta attgtagtga aaaatgggaa gaaggaaacg
      241 attgtccgct ttgaacaata caatcacatg cctttggtta tgcgctta
//
LOCUS       DASOUD010000245          274 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.255, whole genome shotgun sequence.
ACCESSION   DASOUD010000245 DASOUD010000000
VERSION     DASOUD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.255"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>274
                     /locus_tag="U4A29_002732"
     CDS             <1..>274
                     /locus_tag="U4A29_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO0681781.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMA"
BASE COUNT           66 a           56 c           78 g           74 t
ORIGIN      
        1 gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc aatgcggctg
       61 cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt tactatacga
      121 tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa gggtttagtc
      181 atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt gctggcagct
      241 gtccatttca tggcaactgt ttagaaggga tggc
//
LOCUS       DASOUD010000246          270 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.256, whole genome shotgun sequence.
ACCESSION   DASOUD010000246 DASOUD010000000
VERSION     DASOUD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.256"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>270
                     /locus_tag="U4A29_002733"
     CDS             <1..>270
                     /locus_tag="U4A29_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO0681782.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DASOUD010000247          260 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.257, whole genome shotgun sequence.
ACCESSION   DASOUD010000247 DASOUD010000000
VERSION     DASOUD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.257"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>260
                     /locus_tag="U4A29_002734"
     CDS             <1..>260
                     /locus_tag="U4A29_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEO0681783.1"
                     /translation="VTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIK
                     RRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT           86 a           54 c           51 g           69 t
ORIGIN      
        1 ttgtgaccga taaagcacct tctcttggct ccgcctttag aaagttacag agtgtgggtt
       61 tatatactaa gacagagcac cgaactgtga agtatcttaa caatttaata gaacaagacc
      121 atcgacctat taaacgacga aataaatttt atcaaagtct ccgtacagcc tcttccacga
      181 ttaagggcat ggagaccatt cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct
      241 tcggcttttc ggtgtctact
//
LOCUS       DASOUD010000248          241 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.258, whole genome shotgun sequence.
ACCESSION   DASOUD010000248 DASOUD010000000
VERSION     DASOUD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.258"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..220)
                     /locus_tag="U4A29_002735"
     CDS             complement(<1..220)
                     /locus_tag="U4A29_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEO0681784.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DASOUD010000249          227 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.259, whole genome shotgun sequence.
ACCESSION   DASOUD010000249 DASOUD010000000
VERSION     DASOUD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.259"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..227
                     /locus_tag="U4A29_002736"
                     /pseudo
     CDS             1..227
                     /locus_tag="U4A29_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DASOUD010000250          224 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.260, whole genome shotgun sequence.
ACCESSION   DASOUD010000250 DASOUD010000000
VERSION     DASOUD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.260"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>224
                     /locus_tag="U4A29_002737"
     CDS             <1..>224
                     /locus_tag="U4A29_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEO0681785.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DASOUD010000251          216 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.261, whole genome shotgun sequence.
ACCESSION   DASOUD010000251 DASOUD010000000
VERSION     DASOUD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.261"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           75 a           25 c           47 g           69 t
ORIGIN      
        1 ttcttgctcg aaagttatgg aaatttcgat agccgtaggc cacttgtatt gtgcaatagt
       61 tttttacaaa aaaaaggggg tgcggatatt tttttggacc tttttaacaa ataggttctg
      121 ttgcaaagtt ttaaatctac tatcagataa ggtagaataa tagaaaaaga tagcaggagg
      181 aatgacgatg agtcatttta aaggaaagca atttca
//
LOCUS       DASOUD010000252          206 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.denovo.262, whole genome shotgun sequence.
ACCESSION   DASOUD010000252 DASOUD010000000
VERSION     DASOUD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.denovo.262"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>206)
                     /locus_tag="U4A29_002738"
     CDS             complement(<1..>206)
                     /locus_tag="U4A29_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO0681786.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DASOUD010000253         9271 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.guided.088, whole genome shotgun sequence.
ACCESSION   DASOUD010000253 DASOUD010000000
VERSION     DASOUD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.guided.088"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            260..1171
                     /locus_tag="U4A29_002739"
     CDS             260..1171
                     /locus_tag="U4A29_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEO0681787.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ"
                     /locus_tag="U4A29_002740"
     CDS             1324..1809
                     /gene="vanZ"
                     /locus_tag="U4A29_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEO0681788.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="U4A29_002741"
     CDS             2328..2612
                     /locus_tag="U4A29_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0681789.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2978..3277
                     /locus_tag="U4A29_002742"
     CDS             2978..3277
                     /locus_tag="U4A29_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681790.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3320..4810
                     /locus_tag="U4A29_002743"
     CDS             3320..4810
                     /locus_tag="U4A29_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEO0681791.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            5158..5328
                     /locus_tag="U4A29_002744"
     CDS             5158..5328
                     /locus_tag="U4A29_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681792.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            5342..5959
                     /locus_tag="U4A29_002745"
     CDS             5342..5959
                     /locus_tag="U4A29_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0681793.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            5959..6636
                     /locus_tag="U4A29_002746"
                     /pseudo
     CDS             5959..6636
                     /locus_tag="U4A29_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            6947..7444
                     /locus_tag="U4A29_002747"
     CDS             6947..7444
                     /locus_tag="U4A29_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEO0681794.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            7445..7861
                     /locus_tag="U4A29_002748"
     CDS             7445..7861
                     /locus_tag="U4A29_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO0681795.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            7863..>9271
                     /locus_tag="U4A29_002749"
     CDS             7863..>9271
                     /locus_tag="U4A29_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO0681796.1"
                     /translation="MTEKNIMVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNS
                     SYEVQVSHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVIT
                     KSISRFARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQS
                     LSQNVKLGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQS
                     LVGIGRALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKN
                     EGHVPQYYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFC
                     GDCGDIYRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLA
                     GGNNMIKTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDE
                     LRDKRQILLVEDASLSGEN"
BASE COUNT         3392 a         1429 c         1834 g         2616 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa gttaatagaa aataacaaaa taatttattc
     3181 gattagtgga aaaaaattga cttataaagg aaaaaaatct ttttcaaaac atgcaatatt
     3241 gaaacagttg aatgaaaaag caaaccaagt taattaaaca acctatttta taggatttat
     3301 aggaaaggag aacagctgaa tgaatatccc ttttgttgta gaaactgtgc ttcatgacgg
     3361 cttgttaaag tacaaattta aaaatagtaa aattcgctca atcactacca agccaggtaa
     3421 aagcaaaggg gctatttttg cgtatcgctc aaaatcaagc atgattggcg gtcgtggtgt
     3481 tgttctgact tccgaggaag cgattcaaga aaatcaagat acatttacac attggacacc
     3541 caacgtttat cgttatggaa cgtatgcaga cgaaaaccgt tcatacacga aaggacattc
     3601 tgaaaacaat ttaagacaaa tcaatacctt ctttattgat tttgatattc acacggcaaa
     3661 agaaactatt tcagcaagcg atattttaac aaccgctatt gatttaggtt ttatgcctac
     3721 tatgattatc aaatctgata aaggttatca agcatatttt gttttagaaa cgccagtcta
     3781 tgtgacttca aaatcagaat ttaaatctgt caaagcagcc aaaataattt cgcaaaatat
     3841 ccgagaatat tttggaaagt ctttgccagt tgatctaacg tgtaatcatt ttggtattgc
     3901 tcgcatacca agaacggaca atgtagaatt ttttgatcct aattaccgtt attctttcaa
     3961 agaatggcaa gattggtctt tcaaacaaac agataataag ggctttactc gttcaagtct
     4021 aacggtttta agcggtacag aaggcaaaaa acaagtagat gaaccctggt ttaatctctt
     4081 attgcacgaa acgaaatttt caggagaaaa gggtttaata gggcgtaata acgtcatgtt
     4141 taccctctct ttagcctact ttagttcagg ctattcaatc gaaacgtgcg aatataatat
     4201 gtttgagttt aataatcgat tagatcaacc cttagaagaa aaagaagtaa tcaaaattgt
     4261 tagaagtgcc tattcagaaa actatcaagg ggctaatagg gaatacatta ccattctttg
     4321 caaagcttgg gtatcaagtg atttaaccag taaagattta tttgtccgtc aagggtggtt
     4381 taaattcaag aaaaaaagaa gcgaacgtca acgtgttcat ttgtcagaat ggaaagaaga
     4441 tttaatggct tatattagcg aaaaaagcga tgtatacaag ccttatttag tgacgaccaa
     4501 aaaagagatt agagaagtgc taggcattcc tgaacggaca ttagataaat tgctgaaggt
     4561 actgaaggcg aatcaggaaa ttttctttaa gattaaacca ggaagaaatg gtggcattca
     4621 acttgctagt gttaaatcat tgttgctatc gatcattaaa gtaaaaaaag aagaaaaaga
     4681 aagctatata aaggcgctga caaattcttt tgacttagag catacattca ttcaagagac
     4741 tttaaacaag ctagcagaac gccctaaaac ggacacacaa ctcgatttgt ttagctatga
     4801 tacaggctga aaataaaacc cgcactatgc cattacattt atatctatga tacgtgtttg
     4861 ttttttcttt gctgtttagc gaatgattag cagaaatata cagagtaaga ttttaattaa
     4921 ttattagggg gagaaggaga gagtagcccg aaaactttta gttggcttgg actgaacgaa
     4981 gtgagggaaa ggctactaaa acgtcgaggg gcagtgagag cgaagcgaac acttgatttt
     5041 ttaattttct atcttttata ggtcattaga gtatacctat ttgtcctaat atgattttag
     5101 cagtataatt gacttggtga ataggtcatt taagttgggc ataataggag gagtaaaatg
     5161 aaaaaattta tttatcgagt tttagaaaat gacgaagtgg tggctatttt taatgagcaa
     5221 caatatgcgc aagattttat cgcttacgaa aagacaattt ctgataagca atttgaaatt
     5281 gaaaaagtag atattgctga ttggttattg caaccgagag aattttagag gttggttgaa
     5341 aatggctaaa attggttatg cacgtgtcag tagcaaagaa cagaacttag atcggcaatt
     5401 acaagcgtta cagggcgttt ctaaggtctt ttcagacaaa ttaagcggtc aatcggtcga
     5461 acgcccacaa ttacaagcta tgcttaacta tattcgtgaa ggggatattg ttattgttac
     5521 tgaattagat cgattaggac gaaataataa agaattaaca gaattgatga atcaaattca
     5581 aattaagggg gcaaccctgg aagtcttaaa tttaccctca atgaatggta ttgaagatga
     5641 aaatttaagg cgtttgatta atagccttgt cattgaattg tacaagtatc aagcagaatc
     5701 agaacgaaaa aaaattaagg aacgtcaggc acaaggaatc gaaattgcta agaaaaaagg
     5761 caaattcaaa ggtcgtcagc ataaatttaa agaaaatgat ccacgtttaa agcatgcttt
     5821 cgatttgttt ttgaatggtt tatccgataa agaagttgaa gaacaaactg gaatcaatcg
     5881 ccgaacgttt agaaggtatc gagcaagata taacgtgaca gtcgatcaaa gaaaaaacaa
     5941 tgaaaagagg gatagttaat gagtacggtt attttagctg aaaaaccaag ccaggcatta
     6001 gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg gttattttga gatcaaagac
     6061 ccactattta cagatgaaac gtttatcacc tttggttttg ggcatttagt ggaattagca
     6121 gaaccaggtc attatgacga aaagtggcaa aattggaaac ttgaatcttt gccgattttt
     6181 cctgatcgat acgattttga agttgcaaaa gataagggaa agcagtttaa aattgttgct
     6241 gaacttctca aaaaggcaaa tacaattatt gtcgcaacag atagcgacag agaaggtgaa
     6301 aatatcgcct ggtcgattat ccataaagca aatgcctttt ctaaagataa aacgtataaa
     6361 agattatgga tcaatagctt agaaaaagat gtgatccgaa gcggttttca aaatttgcaa
     6421 ccaggaatga attactatcc cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg
     6481 ttgatcggca tgaacgcaag ccctttatat accttgaatt tacaacaaaa aggcgtacaa
     6541 ggtacatttt cattaggacg tgtacaaaca ccgactttat atctcattta tcaacgccag
     6601 gaagcaatag aaaactttaa aaaagaacct tttttcctga ataattcata gttttactaa
     6661 tagtctttcc taacattgat ttaatagtct ttaaagtatt ctgcttcatc acccattagg
     6721 tgatgaaaaa agaacccctt tctgatttag cggacaaata ctttgaccta atctcagaag
     6781 atataaaaga aaaaataata aaatatccga tttatacttt gtataaagaa ctaaagtagt
     6841 tagcaaatca tttacagcaa atcactatgt tccacatacc attttacata cttaccttat
     6901 ccacgacaca tgttgagata gtatcgaaga taaagaaagt tgctccatgt tgtttttgcg
     6961 agatggggaa atcaaagcca cattgacaac catgatgaat aagcttgctt tcagtcataa
     7021 gctaatccta gaaccacttt tcaagtcagt tagccaaatc gatgaagaaa gcgactgtga
     7081 aagaatggac gctattgata agctaatgga gcaactattg gaagaacgca atacccttat
     7141 tgcactgatg tccaaaggtt tcctagaacc tgctcttttt aatcaggaac gaaatgtttt
     7201 agatagtgag attaaaaatc ttactaccga aaaaacaaac ctggtaacga actctgcgag
     7261 tggggttttg cgagcaaacg agataaagga cctcattaat tatgtgtcag cagataattt
     7321 taatggtgac tacacggaag aactatttga agaatttgta gtgaacatca ttgtaaattc
     7381 cagggatgag ctgacattca atttgaaatg cggtctttcc ctgaaagaaa aggtggtgag
     7441 ataaatggca tatattccat atggatacaa aattcaagat ggagtggtta ctgtcgatga
     7501 aaaggcagca ggtcaagtaa aggtattctt tgagaaatac atatcaggac tatcccttac
     7561 agtggctggc gaacaggcag gtattgaaaa gacacattct gtgatggggc gcattttgaa
     7621 aaacgtcaac taccttggaa atgatacgta tccagcaatt attgataaag agatatttga
     7681 taaagctgaa gaagttagag ataaacgtgc aaaggattta ggacgagtgg tagagcttgc
     7741 cgctttcacc tctccccctc ccaaagaacg attcaaaatg aaaaaggcag ataataagat
     7801 gccagtggat ccttttgaac gagcagaata cttatacagt ctgatagaaa gcgaggaata
     7861 aagtgacaga gaaaaatata atggttattc ctgctcgtaa aagagtagga agtacagccg
     7921 caaaagaaaa gataaagaaa cttcgtgttg ctgcctattg ccgtgtttct acagaaacag
     7981 aagaacaaaa ttctagctat gaggttcagg tctcacacta tacagagttt ataaagaaaa
     8041 ataatgaatg ggagtttgct ggcatctttg cagatgatgg tatctctggt acgaacacta
     8101 aaaagcgtga cgaatttaat cgcatgattg cagagtgtat ggacggtaac atcgacatgg
     8161 ttattacaaa atccatcagc cgatttgcgc gtaacaccct agactgcctt caatacatta
     8221 gacagctcaa ggataagaac atatcagttt attttgagaa agagaacatc aacaccacgg
     8281 atgccaaagg tgaggttttg ctgactatta tggcatcttt ggcacagcag gaaagccaga
     8341 gcctttcaca aaacgttaag cttgggctac agtaccgata ccaacaagga aaggtgcagg
     8401 tcaaccataa gcgatttatg ggctacacga aagatgaaga tggaaattta actattgtcc
     8461 ccgaagaagc tgagattatc aaacgcatct accgagaata ccttgagggt cagagtctag
     8521 tgggcattgg tcgagccctt gaaaaggatg gtattttaac agcagcggga aaaccaagat
     8581 ggcgaccaga atcagttaag aagatacttc aaaacgaaaa atacatcgga gatgcccttc
     8641 tgcaaaagac tgtcacagta gattttctga ccaagaaacg agtgaaaaat gaagggcatg
     8701 ttccccaata ttatgttgaa aatagccatg aagcgattat tcctaaagag ttatttttgc
     8761 aggcgcaaga agaaattcat cggagaagca atatctacac aggagaaggc aagaacaaaa
     8821 gaatttacag tagcaagtac gctttaagtg ccatcacctt ctgtggagat tgtggcgata
     8881 tttatagacg ggtctattgg aatatccacg gaagaaaaga atttgtctgg cgatgcgtga
     8941 ctagaatcga gcaaggtcct gaagtttgta agaaccgaac cgtaaaagaa gatgaacttt
     9001 atggtgcagt aatgactgcg attaataagc tacttgcagg tgggaacaat atgataaaga
     9061 cactggaaga gaatattcat gctgtgattg gcgaaaccac agaataccaa atttcagaga
     9121 ttaacaactc actagaggaa aaacaaaaag aactaatcaa gctggcgaac aagggtcaag
     9181 actatgacca cttagcagat gagattgatg agctgagaga caagcgacag atccttttag
     9241 tagaagatgc ctccctcagt ggcgagaacg a
//
LOCUS       DASOUD010000254         5802 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.guided.126, whole genome shotgun sequence.
ACCESSION   DASOUD010000254 DASOUD010000000
VERSION     DASOUD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.guided.126"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..745)
                     /locus_tag="U4A29_002750"
                     /pseudo
     CDS             complement(1..745)
                     /locus_tag="U4A29_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1020..1517
                     /gene="dfrG"
                     /locus_tag="U4A29_002751"
     CDS             1020..1517
                     /gene="dfrG"
                     /locus_tag="U4A29_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEO0681797.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1589..3541)
                     /locus_tag="U4A29_002752"
     CDS             complement(1589..3541)
                     /locus_tag="U4A29_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEO0681798.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3548..3784)
                     /locus_tag="U4A29_002753"
     CDS             complement(3548..3784)
                     /locus_tag="U4A29_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681799.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(4383..5783)
                     /locus_tag="U4A29_002754"
     CDS             complement(4383..5783)
                     /locus_tag="U4A29_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681800.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
BASE COUNT         1897 a          994 c          913 g         1998 t
ORIGIN      
        1 atagttctct tgtcttattg tatcttctat ttttgaaaaa tatcatcgtt aatatcagag
       61 aagagaatat tatttgcaag cttaagatcc gccaaggaaa ttgtaacaat ttggctggcg
      121 tattttgcag aagataccat ggcaccaaac tagttgtagc tagccaagtc aaacacgtac
      181 cataataaat ctttttttct atgtttgtta attttttaaa taaaatcagg ggaatacata
      241 aagataacag accaactagt cccattgagt aagtagttaa gtcgttgttc atagcttcag
      301 tcagactatc gaacacatta gatgcatgag catataaatt tgtcacgcta ggtttgttga
      361 ttttttggta gagcatttgt tcaaacatcg gataccaaaa agcactcgtc agaaccaatg
      421 tcattcctgc cgcactaaat aattttttaa atatagcaat aaaatctgca ttcttgaaac
      481 ctttagaaac caaacgaata aaaataaaaa tgataattaa tgctgtactc attaatgttg
      541 atagtacatg gctgtacact agcaaactca tactcaatgc tagctttaca gacggatact
      601 tttcttcttt tccagagatg atttgataag cataaaataa tacagggata acaagtgtta
      661 ttgcgatagc ttcggcaatt gcggaacgat agtagatgtc tactgtccga taagcagaga
      721 aaatatagag acaactaaat aaaaataagt agttcagttt tggagtacaa aatgacaaaa
      781 tgtacttcaa aatggactac ttttttcatt ttaacttaca taccaaatta catacaaaat
      841 gatttataaa atgcaattga aaatgataat tgaatgaaaa tttgtgattt tgtggtatta
      901 tgataatata acttatagaa aaaattgctt attcaactaa cgggcaggat aatgagaaaa
      961 ggttttttga tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa
     1021 tgaaagtttc tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca
     1081 ttccttggag gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga
     1141 taatattagg taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata
     1201 ttattctgac gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag
     1261 aagatgtttt tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga
     1321 tttataattt gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat
     1381 tcgaaggaga tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc
     1441 aaaaagggat aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa
     1501 aaaacttatt gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag
     1561 agcttgggat tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa
     1621 attgtttatc tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt
     1681 taaaagggcg acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact
     1741 tcggattgaa gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc
     1801 tttcaagaga tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat
     1861 taacatttaa tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac
     1921 aatcaactga ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg
     1981 catatttgcg atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg
     2041 ttagtaattc agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc
     2101 tgatatctcc ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt
     2161 ttctgctttt cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt
     2221 ctttcgtgaa tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc
     2281 gccattcgtc ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag
     2341 gagcatcaac atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt
     2401 gaagataagc attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc
     2461 aatctaacct atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg
     2521 tatcttcatt taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca
     2581 catatcttga taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa
     2641 accccgtata tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca
     2701 tcttgtccgt attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat
     2761 atcgttctaa aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc
     2821 caactccaat tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct
     2881 tagcgaacat tggaagaatg gtgttctttt gttggtgata agtagtggtt tcttctcttt
     2941 ttggtagaac attagtgaac ttcttacacg ccttacattg atagcgttga gatttgcccc
     3001 tacttttccc ccttttgtaa aattgcttcg gctcattgaa tggggttgat tcttcttccg
     3061 agcagccttc tttatgaaat tgataatctg gctcaacatc ttggatgcta ttgatttcta
     3121 taaggcgttt aatttcttca acaacagacc aattagataa aggggttact gtacagtttt
     3181 gcgatacacc tctgccaata ggattgtcat tgcagtacat acctttatga cctttatctt
     3241 tggaactacc agtcatttta tatcgacttg gtttcccttt tacatctttg aatttgtgct
     3301 gttctttccc aaaattacaa caaaacggat tgatgcagta attatattga atgggatatt
     3361 cagaaccgtt ccatttaaaa gacgttggca agaacaacat tttgttatac cttgttgcaa
     3421 atcgttttgg agttaataaa tcatattgtt tagccctatc ttttaattct tcgtttgaaa
     3481 tcggaatttt aacatctaca atttcatctt ccttcgtggc taatctttta agcttggtca
     3541 ttgtctatca acccactcta tctttgatac ccattttttc aacaatccgc ttattacttc
     3601 gcttgttggc aatccattca ataaacttct tatcgtctaa aatgttcaat aagaattcat
     3661 ccactgcttc gctttcagta cgctctgcat attcagcgta ttgcttaact atcaccctta
     3721 cttgctctga tatttcccaa tccacagact tagcgttctt attttttggc tcaagaaatt
     3781 gcataggaaa atgcacccct ttttgcttag taaattgtaa ccattttaac acaccaattt
     3841 gcattcctac aataattatt tcacgttcgc atttttatat acattttaac ttacattttg
     3901 tggtgtaaat taacaacaaa cgttgattta acaataaaaa acgagtatca aattcagttg
     3961 caatttgata ctcatttagg tttacatttt cactttttgt actccaaaac tgaactactt
     4021 aaaataaaaa tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga
     4081 gtgttgtaca caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg
     4141 tcatccaata taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac
     4201 catatccagt ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc
     4261 ccttgattcg agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt
     4321 tgaaaaagat gaaagaatat atactactaa ctataattaa tccactcaat attactactt
     4381 ttttatttat tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc
     4441 aacttattag gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata
     4501 ctgatccata aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt
     4561 tctccagcat ttacatccac attcactaag ccatgttttt tataagagtc atttacgact
     4621 ttaccgttta cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc
     4681 tttttgttgc ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt
     4741 tcccacgatt ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct
     4801 tctgttgggg cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt
     4861 tcttcattga taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt
     4921 gctgctatag acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat
     4981 tttttattca tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt
     5041 tggataccta agatacgcca aggaaactgg agtaagctta tcggtgtttt ttgaaacaaa
     5101 aaccatggaa ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt
     5161 ttttcttttt tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct
     5221 aataacccca tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa
     5281 atagaaagtg cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc
     5341 atctgctgca acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc
     5401 tttactaaat tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa
     5461 cgaactataa tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta
     5521 tacacaagta gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc
     5581 aatccataag cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt
     5641 gcggatcgat aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat
     5701 aaaaaagcag catctatctt tttaaaaaac ttttctccat aataaaaact aatcagaatc
     5761 gtacaaatat ttaataacca aatatataga acataactta at
//
LOCUS       DASOUD010000255         5287 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.guided.131, whole genome shotgun sequence.
ACCESSION   DASOUD010000255 DASOUD010000000
VERSION     DASOUD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.guided.131"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..188
                     /locus_tag="U4A29_002755"
     CDS             <1..188
                     /locus_tag="U4A29_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO0681801.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="U4A29_002756"
     CDS             203..1072
                     /locus_tag="U4A29_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEO0681802.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="U4A29_002757"
     CDS             1053..1787
                     /locus_tag="U4A29_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO0681803.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="U4A29_002758"
     CDS             1820..2728
                     /locus_tag="U4A29_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEO0681804.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3267
                     /gene="sat4"
                     /locus_tag="U4A29_002759"
     CDS             2725..3267
                     /gene="sat4"
                     /locus_tag="U4A29_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEO0681805.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3360..4154
                     /locus_tag="U4A29_002760"
                     /note="aph(3')-IIIa"
     CDS             3360..4154
                     /locus_tag="U4A29_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEO0681806.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4430..5002
                     /locus_tag="U4A29_002761"
     CDS             4430..5002
                     /locus_tag="U4A29_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEO0681807.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         1821 a          795 c         1186 g         1485 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     3001 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     3061 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     3121 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     3181 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     3241 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     3301 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     3361 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     3421 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     3481 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     3541 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     3601 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     3661 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     3721 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     3781 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     3841 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     3901 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     3961 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     4021 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     4081 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     4141 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     4201 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     4261 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     4321 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     4381 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     4441 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     4501 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     4561 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     4621 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     4681 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     4741 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     4801 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     4861 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     4921 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     4981 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     5041 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     5101 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     5161 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
     5221 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
     5281 ttgtgat
//
LOCUS       DASOUD010000256         4801 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.guided.141, whole genome shotgun sequence.
ACCESSION   DASOUD010000256 DASOUD010000000
VERSION     DASOUD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.guided.141"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            92..787
                     /gene="vanR"
                     /locus_tag="U4A29_002762"
     CDS             92..787
                     /gene="vanR"
                     /locus_tag="U4A29_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEO0681808.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="U4A29_002763"
     CDS             765..1919
                     /locus_tag="U4A29_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEO0681809.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="U4A29_002764"
     CDS             2134..3102
                     /locus_tag="U4A29_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEO0681810.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="U4A29_002765"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="U4A29_002765"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEO0681811.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="U4A29_002766"
     CDS             4132..4740
                     /locus_tag="U4A29_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEO0681812.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          920 c         1119 g         1280 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 c
//
LOCUS       DASOUD010000257         3018 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.guided.175, whole genome shotgun sequence.
ACCESSION   DASOUD010000257 DASOUD010000000
VERSION     DASOUD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.guided.175"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..1162
                     /locus_tag="U4A29_002767"
     CDS             41..1162
                     /locus_tag="U4A29_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO0681813.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1420..2922
                     /gene="eat(A)"
                     /locus_tag="U4A29_002768"
     CDS             1420..2922
                     /gene="eat(A)"
                     /locus_tag="U4A29_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEO0681814.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2950..>3018
                     /locus_tag="U4A29_002769"
     CDS             2950..>3018
                     /locus_tag="U4A29_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO0681815.1"
                     /translation="MVKGNFANEGENHMLYVTRHGET"
BASE COUNT         1051 a          519 c          673 g          775 t
ORIGIN      
        1 aaaaaaagtg tcttacttaa gaagaaagtg agggatacag atggatattt tggcggcaat
       61 tgattcattt aaaggaagtg cttcttcatt tgaactaaat caggcagttc taaatggctg
      121 ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca gatggcggag aaaatactgc
      181 agaagcaatc tattacgcgc tcaggggaac atggcgtacc attgatggga tggatctctt
      241 attccgtcag cgaaaaatca gctatttatt gacaacttgg caaggaaaaa acaccgcagt
      301 catcgaatct gcacaagcat tggggttaga tctacttgat aaaccaacgg atcaaactat
      361 ccgacttgct tccagttatg ccttaggaga acttattaaa gatgcactgc ttcaagaagt
      421 agaacaaatt attgtcactt taggaggaag cgggtgtagc gatggtggtc taggattact
      481 ccaatccttg ggtgcttctt tagaagggta cacagaagga aatcctttat tatctactaa
      541 aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa gtaaaattga ttatcgctgc
      601 agatgttacc aatccatata caggtcaaca aggagctgct tggcaatttg gaagacaaaa
      661 aggaggaata gatacgacgc tagctttctt agataaacaa gcaggaaaga ttgctcagca
      721 aataaaagat ttctgggagg tggatttaga tgatattcca ggaagtggag cagcaggcgg
      781 tattggtgga gccctttttt tgttgggagc gaagatggtt tctggtttcg atttagtcag
      841 ccaaatcact ggattggaaa aaaagatcga acaagcagat gtcatcatta caggagaagg
      901 gcggatcgat cagcaaacga tccacggaaa agttccatac ggtgtagcag tgcttgcaaa
      961 aaaatatcga aaaccaataa tcgctatttg cggaagtaga gaccaagagc tagaaggact
     1021 ttctgatttt ctcccaattg tcttaagtat tcatttgggt ccaataggtt tagaagaagc
     1081 aatggaacac aggcaaacat tagaaaaaac aaaaatctta ggacagacac ttagcagatt
     1141 tttcaccatc ctcccgaagt agttgacaaa atcattttct ttatgctatc gtaatttcaa
     1201 atataggaca aagtgaggga tccgtgcatt gaaaaattaa gtcgattgat gaaagctgca
     1261 ttcagactgt accttgtgga gacaaggaag cagtgtgcag attttcggga ttggcttggt
     1321 tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gtatcctctt tagccagttc
     1381 tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa
     1441 aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc aaatctaaat
     1501 tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa gacaacttta
     1561 ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca gcaagaattt
     1621 gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt gttaaatgat
     1681 attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca aacagatcct
     1741 gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa agtcctactg
     1801 gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac gaatcatttg
     1861 gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca aggcttcatc
     1921 gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt agcaatcgaa
     1981 aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca gaaaaaactt
     2041 cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag
     2101 aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa aacaaagaaa
     2161 caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt gggtgctgat
     2221 gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac ccagatcagc
     2281 gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat gaatagccaa
     2341 gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta tgaaaatctg
     2401 ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat ttcaggtcct
     2461 aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa cggcaaggtt
     2521 ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca aaattatgaa
     2581 gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta ccaagcattt
     2641 ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa gatcgagcag
     2701 atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca gccagctgaa
     2761 ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga acaattagaa
     2821 caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca aaccttcctg
     2881 gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta atctcaagaa
     2941 caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat gctttatgta
     3001 acgagacacg gagaaaca
//
LOCUS       DASOUD010000258         2508 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.guided.179, whole genome shotgun sequence.
ACCESSION   DASOUD010000258 DASOUD010000000
VERSION     DASOUD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.guided.179"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            55..792
                     /gene="erm(B)"
                     /locus_tag="U4A29_002770"
     CDS             55..792
                     /gene="erm(B)"
                     /locus_tag="U4A29_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEO0681816.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1055..1918)
                     /locus_tag="U4A29_002771"
     CDS             complement(1055..1918)
                     /locus_tag="U4A29_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEO0681817.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(1920..2192)
                     /locus_tag="U4A29_002772"
     CDS             complement(1920..2192)
                     /locus_tag="U4A29_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEO0681818.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2209..2418)
                     /locus_tag="U4A29_002773"
     CDS             complement(2209..2418)
                     /locus_tag="U4A29_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEO0681819.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          743 a          455 c          403 g          907 t
ORIGIN      
        1 aatcgtttaa cttctgtatt tatttataga tgtaatcact tcaggagtga ttacatgaac
       61 aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa ccaaataata
      121 aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg taaagggcat
      181 ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt agacagtcat
      241 ctattcaact tatcgtcaga aaaattaaaa ctgaacactc gtgtcacttt aattcaccaa
      301 gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg gaatattcct
      361 taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccatgc gtctgacatc
      421 tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg aacactaggg
      481 ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc ggaatgcttt
      541 catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac cacagatgtt
      601 ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa tcgagaatat
      661 cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa agtaaacaat
      721 ttaagtaccg ttacttatga gcaagtattg tctattttta atagttatct attatttaac
      781 gggaggaaat aattctatga gtcgcttttg taaatttgga aagttacacg ttactaaagg
      841 gaatgtagat aaattattag gtatagggca cctctaataa ctaccaatta attcacctca
      901 aaatgaaatt agaaaaaaac agcgaaattt ttaaatctat ttcttatcga tacaaattcc
      961 tcgtaggcgc tcgggacccc aacatgagga aatgttttta taaaaaaaga gtagttaccg
     1021 ttttttggta actactcttt tcctttttta aggtttaaat acctggaagt ttaggtgttt
     1081 tgggtattgg tggtgtaggt agctgcaagc tttccatttt ttgttgaatt gctttaaatt
     1141 caggggtttc ttggtgttga ttttgaacca ttttttgctc tattctctcc aaagtcggtt
     1201 gaatttcttt ccctgatact ttacgattta attctctttc taaggtctct ttcggactaa
     1261 tggaaggcgt ttctaagctt gaatagagtt tcactccttc tctattgtaa agccttatat
     1321 cgctaaataa gcccgtttta tgaagggttt ctaaattggt cggtaagttt ttgacaacaa
     1381 tatcatgcgc ttgttttggt gttgccctag ctgtcattgg atcatctgca tacatggttt
     1441 catatcgttc aatcgttcct aagtatgatt caattttcgg tactgccatg acgtatattt
     1501 ttgtttcata acctttagat tgaagcattg tggctgtttg aatgggaacg tctgttgttc
     1561 gtcctgttcc ttcaatcact aaattatagc cttgatcgct taaacggcta ataagtgctt
     1621 ctgtcatgcg attcgaataa ggggtaacgt gttttactac gtctttttca tagagtttca
     1681 ctaattcgtc aaaatttggg tgctgttgtt taaaggtatc attatcaatg acaataacat
     1741 tcccttgtgt ttcttcaaaa attgctgatc gcaaactggt tttccctgac cctggttgcc
     1801 caccaagtaa aaaagcggtt ggcgattcaa ccgctagtct atttttagtt agtcgttcaa
     1861 cattcttttc taaacgatct tcaaattgct tttcggtgaa gtcagtaata ttcgccatgt
     1921 taagccactt tctctttatt caaaatttgt tttgaatatt gattcattga ccgccaatac
     1981 tcatgaatag cttgtaattc ttcaaatggt tgttgaaata aatcaacttc ttgtgccact
     2041 tctaattgat ttaaaagatt cacttctagt aaacgagtat ttttagtatc tagttcatga
     2101 ttcaaaacat aatttaatac ccgattataa acacttgccg ataactcatt aatgatttca
     2161 atttcaaatg ttttttcata cgtaactgcc atttcatttt cactcctttt aaagtttgtc
     2221 aggtaaatat ttccgtatat attcttctaa ggcttgatcc ataacttctt ttacgtttcc
     2281 gccattcttt gctgtttcga tttttatgat atggtgcaag tcggcacgaa cacgaaccgt
     2341 cttatccccc attatatctt tttttgcgct gattggcgta tcatttcgtt ttgctttctg
     2401 tgcgcctaaa tttcccacga tcgctcactt ctttctattt cttcttacta tttattttat
     2461 catcacctat cacaaatcac aagtgatttg tgattaatca cttgtgat
//
LOCUS       DASOUD010000259         2367 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.guided.183, whole genome shotgun sequence.
ACCESSION   DASOUD010000259 DASOUD010000000
VERSION     DASOUD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.guided.183"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(40..342)
                     /locus_tag="U4A29_002774"
                     /pseudo
     CDS             complement(40..342)
                     /locus_tag="U4A29_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1056..1346)
                     /locus_tag="U4A29_002775"
     CDS             complement(1056..1346)
                     /locus_tag="U4A29_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO0681820.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1554..2246)
                     /locus_tag="U4A29_002776"
     CDS             complement(1554..2246)
                     /locus_tag="U4A29_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO0681821.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          685 a          444 c          337 g          901 t
ORIGIN      
        1 tatggatttt ttcatttaca caaaatattt tatactctca acatacttgc aaagtaatac
       61 tacaattcct aacataatcg tagaatttaa taatttccat agcataaagt gtttagaaaa
      121 gaacattaag aatagttcta taataattct tgaactccat gtcttataat gttctatcga
      181 agcacccaaa tagccaagtt catctaatct acttgcataa actacgtcat ccgctctcat
      241 agtcggcata tacatattaa agagtaaaaa gataccccaa atccctatta gtaacaagat
      301 cagttctttg ttattcgtta tttttctatt atgtttgttc atttttatac tccttcttat
      361 atttcctaat agagtataac atagctttaa gcattactta aagataactt aaaagttatc
      421 ttttaaatac ttaattgtta aataccttat ttgatgcaaa agaaatctac gatagtcaac
      481 aaaaaaattt tcattatatc cagaaaaatt tttcttagtt aaggacctat ggattgcagt
      541 aatgcagaat tcgtcgaata aactgccaaa gacatacaat catattgctt ttaaaattga
      601 cgaacaagat atagattcgt ttgtctcaaa aatccaaatg ttaggtttaa ctgtcgaacc
      661 aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac ttttacgact atgataatca
      721 cctcttcgag ttacataccg gtactctcca ggaacgttta aaatcctaca attccactac
      781 ctgagactag taatgactag tctcttttta tggcctctga tccccccatt gagcgaatac
      841 taatagggct tcgaattgat ctgttcaatc tttaagcaat atccctcaaa tgagtttgga
      901 aagtttagtg attcatcaca aatacttaaa tacatgtagt tatatataga ttgaggttct
      961 tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg catccatctt tccaaaacaa
     1021 gcggctagga catttttgtc tcagccgctt gttttctagt ttattctttc atcttgtcgg
     1081 accttttttc ttgggtatag acttttctca tattctacca caattttggg tttccctaac
     1141 acatgatagg atctcacaac aaattgcttt ctattattat aataacctgt aacgactaac
     1201 ttcatccctt cgtctacttc agctaggaag ttcaaactat gggaagcaat caaacaattg
     1261 accccattta aactgaaacg gatcaacggt ctggcactaa cttgcaaagt acggatctta
     1321 tctactgttc catatttttt ctccataacc ttcacctcaa taccattata cgaacataca
     1381 ttccctttgt aaagaagaaa ctctgtttta tctttttgac tttttgtcta ttacctttct
     1441 taaaatcata ttggcacttc ctgcgaagat gaaggacatg tactaaaaaa aagaggcagc
     1501 agtatagatt actgttgctt ctttactaat ttaggtttac ttgttaagaa agattattta
     1561 cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg tatcttccac ttcatctttt
     1621 gctttttcta acttttgaat atattgtttc aagcttgagt tttcagcagt tgcttcgtct
     1681 aatttctttt gtgtatctgt taactgagtt tctacatttg ttttttcttg tgtcagtgtt
     1741 gcgatttgtt gattcaaacc atttacagta ttttgtaatt ctgaaagttt tgcttgtgct
     1801 tcttgtagtc ttgctgtcca atctgcattc agatgattga tttcatcatt tttttggttg
     1861 atctcatcta gtttgacatt gatttcattt tgtttgcttt gtagttgtgc atccttgctg
     1921 actaattgac tttctaagtt ttgtttttct gctgccgatt gatttttttg gttttctaat
     1981 tcatttactt ttgtttggta ttcttggaca tctgcctttg aatcttgcaa tgtctgttct
     2041 ttttgtgcta gtcgagcggt aagatctgta atcgcttgct ctttatctgt caatgtatga
     2101 tcaagctgtg tcagattctc attgattgta tcaatatctg tctctccacc ccacaaaata
     2161 atttcatttg cgtaccgcgt gcctgtgtag ccaattagac atagaaatgc aacgacaatc
     2221 ccaccagtaa taaatttctt tttcatttgc atagctcctt aatccaatac ttttacatgc
     2281 taaaagtaag tgatctcaaa cacaatgtca agcgttttta aattacatat gtaattttaa
     2341 tataaatact atcaaatact catttaa
//
LOCUS       DASOUD010000260         2296 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.guided.184, whole genome shotgun sequence.
ACCESSION   DASOUD010000260 DASOUD010000000
VERSION     DASOUD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.guided.184"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            230..1708
                     /gene="msr(C)"
                     /locus_tag="U4A29_002777"
     CDS             230..1708
                     /gene="msr(C)"
                     /locus_tag="U4A29_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEO0681822.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1755..2201
                     /locus_tag="U4A29_002778"
     CDS             1755..2201
                     /locus_tag="U4A29_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEO0681823.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT          773 a          373 c          491 g          659 t
ORIGIN      
        1 ctaaataaat tcaaaagaca tgtataaaca acaatttcat ggatggagta tgtccatttt
       61 ttcgaacgac agtagaatag gtgaaagcta tgacagattt ggtgatttag tcatagttat
      121 ttcgttcaga gtaggaaaag atgtgatgca gtttttttgc aaacagattt ttctgctctt
      181 ttttagtatc gataattgtt gttttacatg aaataggagt gtaaaacata tggaaaattt
      241 agcagtaaat ataacaaatc tacaagtcag ctttggcaac cagctagaac tatctattga
      301 ttctcttcgt gtctatcagc aagatcgtat agggatcata ggagaaaatg gtgtcggtaa
      361 atcgactttg ctcaaactaa tagccggtga actttttccc gatcatggga aaatccaaac
      421 agagatcacc ttcaactacc tgcctcaatt aacctatctc gctgaagcaa aggacctaaa
      481 tttggaatta gccagtcatt tccagttaag actggaagaa acttcagagc ggaaatggag
      541 cggaggggaa gaacgaaaga tcgagttgat acgtcttctt tcttcttatg aacaagggat
      601 gcttctagac gagccaacaa cccatctaga tagaaaaagt attgatcgac tgattgaaga
      661 gcttcgttat tattatggta cgctggtttt tgttagtcat gatcgctatt ttctagatga
      721 gctagcatcg aaaatctggg aagtaaaaga cggagaaatc cgagagtttt cggggaatta
      781 tagtgcctat ctcactcaaa aggaattgga gaaaaagact cagctacgag aagcagagtc
      841 gatcatgaaa gagaaaaaac gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga
      901 aaagttggaa aaagtgtcca gtaaaaagaa aaagcaacaa atcagaccgg atcggttgtc
      961 ttcctctaaa caaaaagaca gtgtacaaaa agccatccaa aagaatgcga aaacattaga
     1021 gagaagactc caaaaaatag gagaaacaac caagccgcag cagatgaaac aaatccgttt
     1081 tccagtacca aaatctcttg aactccacag ccgctatccc atcatgggac aaaatgtcca
     1141 attggaaaga agtgggagaa cattactggt aaatggtgat tttcagtttt ctttaggtaa
     1201 aaaaatcgcg attgtcggtg aaaatggttc aggtaagaca accttattag aacatatccg
     1261 caaacaagga gaaggaatcc ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa
     1321 gggttatcaa atgacatctg aagaatccat cattcgtttt gtcatgagac aaacagagtt
     1381 ttcagaatcg cttgtccgta gtttgctgaa tcacttaggg tttgctcagg aaactttgac
     1441 gaaaccgtta tgtacattaa gtgggggaga agcgacccgt ctgacgattg ctttgctttt
     1501 tactaagcca agtaatgtgt tgctgttaga tgaaccgact aattttattg atatggcaac
     1561 gatcgaagct ttagagaagc taatgcaaat atatccggga acgattttgt ttacttctca
     1621 tgattcttac tttgtcgagc gtacggctga tgaagtttat gaaataaaag ggcagaaaat
     1681 aaaaaaagta cttacgagaa atttttaaat gtgctacgat gattttcgtg acaaatttaa
     1741 aggagtggta cagattgcct acaaataaaa aagaaggaat aatttttaca acaatgatgt
     1801 gttttttgat ggtcttgggg atgagcgcgt ataatttatg gctgcatcaa gatcttcatt
     1861 tatcaagttt gttgattggt ctagtgcctg gattcgtggt ggctttcatt ttggatgtct
     1921 tcatcatcgg tgtgctggcg aaaaaaattg cctttaaact accagtaaat ccagaaagca
     1981 aggtccaaat gatcttaact atttcatgct tgatggtttt aggaatggtg acatgtatgt
     2041 ctttattcgg tgtgatcatg gaacaaggaa tacctgatca attattctct gcttatatga
     2101 atgcttggaa aatgaatgtg attgccgctt taccgttgca gttgatcatc gttggcccgc
     2161 tttcaagaag tgtgctaaaa gcaattcaaa gtaatgaatg atggaacact gttatttgaa
     2221 aaaaggcctt ggcatgcttt ttgtcatagt ctcttgaatg cagttctaga aaaatagtga
     2281 tccgaacgat aggttt
//
LOCUS       DASOUD010000261          913 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.guided.221, whole genome shotgun sequence.
ACCESSION   DASOUD010000261 DASOUD010000000
VERSION     DASOUD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.guided.221"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..787
                     /gene="aph(2'')-I"
                     /locus_tag="U4A29_002779"
                     /pseudo
     CDS             50..787
                     /gene="aph(2'')-I"
                     /locus_tag="U4A29_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
BASE COUNT          392 a           70 c          149 g          302 t
ORIGIN      
        1 tggtaaaaac attgtatagc attttacaca ggagtctgga cttgactaaa aaggttatgc
       61 aaaagaaaaa gcaatatata attttttaaa tacaaattta gaaactaatg taaaaattcc
      121 taatattgaa tattcgtata ttagtgatga attatctata ctaggttata aagaaattaa
      181 aggaactttt ttaacaccag aaatttattc tactatgtca gaagaagaac aaaatttgtt
      241 aaaacgagat attgccagtt ttttaagaca aatgcacggt ttagattata cagatattag
      301 tgaatgtact attgataata aacaaaatgt attagaagag tatatattgt tgcgtgaaac
      361 tatttataat gatttaactg atatagaaaa agattatata gaaagtttta tggaaagact
      421 aaatgcaaca acagtttttg agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa
      481 tcatctattg ttagatggca ataatagatt aactggaata attgattttg gagattctgg
      541 aattatagat gaatattgtg attttatata cttacttgaa gatagtgaag aagaaatagg
      601 aacaaatttt ggagaagata tattaagaat gtatggaaat atagatattg agaaagcaaa
      661 agaatatcaa gatatagttg aagaatatta tcctattgaa actattgttt atggaattaa
      721 aaatattaaa caggaattta tcgaaaatgg tagaaaagaa atttataaaa ggacttataa
      781 agattgatta tataatatat gaaaagctat tataaaagac attagtatta aatagtttaa
      841 aaaaatgaaa aataataaag gaagtgagtc aattccagac tcctgtgtaa aatgctatac
      901 aatgttttta cca
//
LOCUS       DASOUD010000262          765 bp    DNA     linear   BCT 21-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1678
            SAMN38756165-rid20572503.guided.226, whole genome shotgun sequence.
ACCESSION   DASOUD010000262 DASOUD010000000
VERSION     DASOUD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756165
            Sequence Read Archive: SRR27168076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028232.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:46:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,780
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 26 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 17 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756165-rid20572503.guided.226"
                     /isolate="VE1678"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-06-03"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..671
                     /gene="aac(6')"
                     /locus_tag="U4A29_002780"
     CDS             123..671
                     /gene="aac(6')"
                     /locus_tag="U4A29_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEO0681824.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          279 a          114 c          169 g          203 t
ORIGIN      
        1 ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa agatttttga
       61 gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac
      121 gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt
      181 tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa
      241 tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta
      301 ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa
      361 gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag
      421 cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt
      481 taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc
      541 gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa
      601 atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa
      661 attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa
      721 tttacgaaaa ttacttcttg aatttttgtg aaattccaac ttatt
//
